Publications by authors named "Yuan Fang"

1,459 Publications

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Toward a quantitative theoretical method for infrared and Raman spectroscopic studies on single-crystal electrode/liquid interfaces.

Chem Sci 2019 Dec 10;11(5):1425-1430. Epub 2019 Dec 10.

State Key Laboratory of Physical Chemistry of Solid Surfaces (PCOSS), Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China

electrochemical infrared spectroscopy and Raman spectroscopy are powerful tools for probing potential-dependent adstructures at solid/liquid electrochemical interfaces. However, it is very difficult to quantitatively interpret the observed spectral features including potential-dependent vibrational frequency and spectral intensity, even from model systems such as single-crystal electrode/liquid interfaces. The clear understanding of electrochemical vibrational spectra has remained as a fundamental issue for four decades. Here, we have developed a method to combine computational vibrational spectroscopy tools with interfacial electrochemical models to accurately calculate the infrared and Raman spectra. We found that the solvation model and high precision level in the self-consistent-field convergence are critical elements to realize quantitative spectral predictions. This method's predictive power is verified by analysis of a classic spectroelectrochemical system, saturated CO molecules electro-adsorbed on a Pt(111) electrode. We expect that this method will pave the way to precisely reveal the physicochemical mechanism in some electrochemical processes such as electrocatalytic reactions.
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http://dx.doi.org/10.1039/c9sc05429dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148070PMC
December 2019

Anisotropic c-f Hybridization in the Ferromagnetic Quantum Critical Metal CeRh_{6}Ge_{4}.

Phys Rev Lett 2021 May;126(21):216406

Center for Correlated Matter and Department of Physics, Zhejiang University, Hangzhou 310058, China.

Heavy fermion compounds exhibiting a ferromagnetic quantum critical point have attracted considerable interest. Common to two known cases, i.e., CeRh_{6}Ge_{4} and YbNi_{4}P_{2}, is that the 4f moments reside along chains with a large interchain distance, exhibiting strong magnetic anisotropy that was proposed to be vital for the ferromagnetic quantum criticality. Here, we report an angle-resolved photoemission study on CeRh_{6}Ge_{4} in which we observe sharp momentum-dependent 4f bands and clear bending of the conduction bands near the Fermi level, indicating considerable hybridization between conduction and 4f electrons. The extracted hybridization strength is anisotropic in momentum space and is obviously stronger along the Ce chain direction.The hybridized 4f bands persist up to high temperatures, and the evolution of their intensity shows clear band dependence. Our results provide spectroscopic evidence for anisotropic hybridization between conduction and 4f electrons in CeRh_{6}Ge_{4}, which could be important for understanding the electronic origin of the ferromagnetic quantum criticality.
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http://dx.doi.org/10.1103/PhysRevLett.126.216406DOI Listing
May 2021

Acute kidney disease in hospitalized acute kidney injury patients.

PeerJ 2021 24;9:e11400. Epub 2021 May 24.

Department of Nephrology, The Second Xiangya Hospital of Central South University; Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan, China.

Background: Acute kidney injury (AKI) and chronic kidney disease (CKD) have become worldwide public health problems, but little information is known about the epidemiology of acute kidney disease (AKD)-a state in between AKI and CKD. We aimed to explore the incidence and outcomes of hospitalized patients with AKD after AKI, and investigate the prognostic value of AKD in predicting 30-day and one-year adverse outcomes.

Methods: A total of 2,556 hospitalized AKI patients were identified from three tertiary hospitals in China in 2015 and followed up for one year.AKD and AKD stage were defined according to the consensus report of the Acute Disease Quality Initiative 16 workgroup. Multivariable regression analyses adjusted for confounding variables were used to examine the association of AKD with adverse outcomes.

Results: AKD occurred in 45.4% (1161/2556) of all AKI patients, 14.5% (141/971) of AKI stage 1 patients, 44.6% (308/691) of AKI stage 2 patients and 79.6% (712/894) of AKI stage 3 patients. AKD stage 1 conferred a greater risk of Major Adverse Kidney Events within 30 days (MAKE30) (odds ratio [OR], 2.36; 95% confidence interval 95% CI [1.66-3.36]) than AKD stage 0 but the association only maintained in AKI stage 3 when patients were stratified by AKI stage. However, compared with AKD stage 0, AKD stage 2-3 was associated with higher risks of both MAKE30 and one-year chronic dialysis and mortality independent of the effects of AKI stage with OR being 31.35 (95% CI [23.42-41.98]) and 2.68 (95% CI [2.07-3.48]) respectively. The association between AKD stage and adverse outcomes in 30 days and one year was not significantly changed in critically ill and non-critically ill AKI patients. The results indicated that AKD is common among hospitalized AKI patients. AKD stage 2-3 provides additional information in predicting 30-day and one-year adverse outcomes over AKI stage. Enhanced follow-up of renal function of these patients may be warranted.
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http://dx.doi.org/10.7717/peerj.11400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158174PMC
May 2021

Inhibition of Nrf2-mediated glucose metabolism by brusatol synergistically sensitizes acute myeloid leukemia to Ara-C.

Biomed Pharmacother 2021 Jun 8:111652. Epub 2021 Jun 8.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, PR China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, 110 Xiangya Road, Changsha 410078, PR China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha 410078, PR China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Center South University, Changsha 410008, Hunan, PR China. Electronic address:

Chemotherapy resistance remains to be the primary barrier to acute myeloid leukemia (AML) treatment failure. Nuclear factor-erythroid 2-related factor 2 (Nrf2) has been well established as a truly pleiotropic transcription factor. Inhibition of Nrf2 function increases the sensitivity of various chemotherapeutics and overcomes chemoresistance effectively. Brusatol (Bru) has been reported to decrease Nrf2 protein expression specifically by ubiquitin degradation of Nrf2. However, it remains elusive whether combination of Brusatol and Cytarabine (Ara-C) elicits a synergistic antitumor effect in AML. Our results demonstrated that combination of Ara-C and Brusatol synergistically exerted remarkable pro-apoptosis effect in HL-60 and THP-1 cells. Mechanistically, synergistic anti-tumor effect of Ara-C/Brusatol in AML cells is mediated by attenuating Nrf2 expression. To our surprise, Nrf2 inhibition by Brusatol causes downregulation of the expression of glycolysis-related proteins and decreased glucose consumption and lactate production, whereas the level of ROS production was unaffected. The activation of Nrf2 by Sulforaphane (SFP) could reverse the chemotherapeutic effect and changes of glycolysis of concomitant of Ara-C with Brusatol in AML cell lines. Additionally, Ara-C/Brusatol co-treatment decreased Glucose-6-phosphate dehydrogenase (G6PD) protein expression and increased the sensitivity of Ara-C. Moreover, the mouse xenograft in vivo experiment confirmed that combining Ara-C with Brusatol exerted stronger antileukemia than Ara-C alone. The efficacy, together with the mechanistic observations, reveals the potential of simultaneously giving these two drugs and provides a rational basis for targeting glucose catabolism in future clinical therapeutic approach.
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http://dx.doi.org/10.1016/j.biopha.2021.111652DOI Listing
June 2021

The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression.

Nucleic Acids Res 2021 Jun 9. Epub 2021 Jun 9.

Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.

Epigenetics, especially histone marks, functions beyond the DNA sequences to regulate gene expression. Depletion of NSD1, which catalyzes H3K36me2, leads to both up- and down-regulation of gene expression, indicating NSD1 is associated with both active and repressed gene expression. It's known that NSD1 regulates the deposition and expansion of H3K27me3, a repressive mark for gene expression, to keep active gene transcription. However, how NSD1 functions to repress gene expression is largely unknown. Here, we find that, when NSD1 is knocked out in mouse embryonic stem cells (mESCs), H3K27ac increases correlatively with the decrease of H3K36me2 at active enhancers, which is associated with mesoderm differentiation genes, leading to elevated gene expression. Mechanistically, NSD1 recruits HDAC1, the deacetylase of H3K27ac, to chromatin. Moreover, HDAC1 knockout (KO) recapitulates the increase of H3K27ac at active enhancers as the NSD1 depletion. Together, we propose that NSD1 deposits H3K36me2 and recruits HDAC1 at active enhancers to serve as a 'safeguard', preventing further activation of active enhancer-associated genes.
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http://dx.doi.org/10.1093/nar/gkab473DOI Listing
June 2021

Identification of a recurrent missense mutation in the FGA gene likely causing a congenital fibrinogen disorder.

Blood Coagul Fibrinolysis 2021 Jun 7. Epub 2021 Jun 7.

Department of Laboratory Medicine, Jiaozuo Women's and Children's Hospital Department of Laboratory Medicine, Jiaozuo Hospital of Traditional Chinese Medicine, Jiaozuo Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1097/MBC.0000000000001055DOI Listing
June 2021

Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury.

Neural Regen Res 2022 Jan;17(1):130-136

Department of Neurosurgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Our previous study showed that cell cycle exit and neuronal differentiation 1 (CEND1) may participate in neural stem cell cycle exit and oriented differentiation. However, whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear. In this study, we performed quantitative proteomic analysis and found that after traumatic brain injury, CEND1 expression was downregulated in mouse brain tissue. Three days after traumatic brain injury, we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site. We found that at 5 weeks after traumatic brain injury, transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function. In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells, but significantly promoted their neuronal differentiation. Additionally, CEND1 overexpression reduced protein levels of Notch1 and cyclin D1, but increased levels of p21 in CEND1-transfected neural stem cells. Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection. These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury. This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University, China (approval No. 2016034) on November 25, 2016.
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http://dx.doi.org/10.4103/1673-5374.314316DOI Listing
January 2022

Platform study of genotyping-guided precision medicine for rare solid tumours: a study protocol for a phase II, non-randomised, 18-month, open-label, multiarm, single-centre clinical trial testing the safety and efficacy of multiple Chinese-approved targeted drugs and PD-1 inhibitors in the treatment of metastatic rare tumours.

BMJ Open 2021 06 3;11(6):e044543. Epub 2021 Jun 3.

Clinical Trial Center, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Cancer Hospital Chinese Academy of Medical Sciences, Chaoyang District, Beijing, China

Introduction: Limited clinical studies have been conducted on rare solid tumours, and there are few guidelines on the diagnosis and treatment, including experiences with targeted therapy and immunotherapy, of rare solid tumours in China, resulting in limited treatment options and poor outcomes. This study first proposes a definition of rare tumours and is designed to test the preliminary efficacy of targeted and immunotherapy drugs for the treatment of rare tumours.

Methods And Analysis: This is a phase II, open-label, non-randomised, multiarm, single-centre clinical trial in patients with advanced rare solid tumours who failed standard treatment; the study aims to evaluate the safety and efficacy of targeted drugs in patients with advanced rare solid tumours with corresponding actionable alterations, as well as the safety and efficacy of immune checkpoint (programmed death receptor inhibitor 1, PD-1) inhibitors in patients with advanced rare solid tumours without actionable alterations. Patients with advanced rare tumours who fail standardised treatment and carry actionable alterations (Epidermal growth factor receptor (EGFR) mutations, ALK gene fusions, ROS-1 gene fusions, C-MET gene amplifications/mutations, BRAF mutations, CDKN2A mutations, BRCA1/2 mutations, HER-2 mutations/overexpressions/amplifications or C-KIT mutations) will be enrolled in the targeted therapy arm and be given the corresponding targeted drugs. Patients without actionable alterations will be enrolled in the PD-1 inhibitor arm and be treated with sintilimab. After the patients treated with vemurafenib, niraparib and palbociclib acquire resistance, they will receive combination treatment with sintilimab or atezolizumab. With the use of Simon's two-stage Minimax design, and the sample size was estimated to be 770. The primary endpoint of this study is the objective response rate. The secondary endpoints are progression-free survival in the targeted treatment group and single-agent immunotherapy group; the duration of response in the targeted therapy and single-agent immunotherapy groups; durable clinical benefit in the single-agent immunotherapy group; and the incidence of adverse events.

Ethics And Dissemination: Ethics approval was obtained from the Chinese Academy of Medical Sciences (ID: 20/132-2328). The results from this study will be actively disseminated through manuscript publications and conference presentations.

Trial Registration Numbers: NCT04423185; ChiCTR2000039310.
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http://dx.doi.org/10.1136/bmjopen-2020-044543DOI Listing
June 2021

Enhanced nitrogen removal in an electrochemically coupled biochar-amended constructed wetland microcosms: The interactive effects of biochar and electrochemistry.

Sci Total Environ 2021 May 15;789:147761. Epub 2021 May 15.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

The interactive effects of both biochar (BC) and electrochemistry (EC) can affect nitrogen (N) removal process. However, little is known about how this function in constructed wetland (CW) systems. In this study, an electrochemically (EC) coupled BC-amended saturated subsurface vertical flow constructed wetland (BECW) systems were established to enhance nitrogen (N) removal. Other three CW systems: without BC and EC (CW); with EC only (ECW); and with BC only (BCW) were performed as controls. Results indicated that the total nitrogen (59.88%-93.03%) and nitrate‑nitrogen (83.14%-100%) of the BECW system were significantly enhanced (p < 0.05) compared with the control systems. Treated WWTP tail-water could meet Class-IV of the Surface Water Quality Standard (GB3838-2002) in China by the BECW system. The enhanced N removal in the BECW system could be attributed to (1) the autotrophic denitrification process in which H and Fe provided by the cathode and anode acted as electron donors; and (2) BC addition acting as substrate could improve the activity, diversity and richness of microorganisms. Microbial community analysis further indicated that high N removal in the BECW system was significantly dependent on the synergy between the heterotrophic and autotrophic denitrifiers, facilitated by BC and EC interaction. Results illustrate that the BECW system is a feasible and eco-sustainable technology for treating low C/N tail-water from WWTPs. This work provides a novel and fundamental understanding of the electrochemically coupled biochar-amended CW system. These results could serve as a theoretical basis for the engineered applications in the deep purification of WWTPs' tail-water.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147761DOI Listing
May 2021

Corrigendum: E3 Ubiquitin Ligase UBR5 Promotes the Metastasis of Pancreatic Cancer Destabilizing F-Actin Capping Protein CAPZA1.

Front Oncol 2021 11;11:693855. Epub 2021 May 11.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

[This corrects the article DOI: 10.3389/fonc.2021.634167.].
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http://dx.doi.org/10.3389/fonc.2021.693855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147866PMC
May 2021

Recurrent radiation-associated leiomyosarcoma after postoperative radiotherapy for breast invasive ductal carcinoma: a case report and literature review.

Ann Palliat Med 2021 May 19. Epub 2021 May 19.

Department of Thyroid & Mammary Gland Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Radiation-associated leiomyosarcoma has a poor prognosis. Fewer than 20 cases of radiationassociated leiomyosarcoma of the breast have been reported in the literature to date. We report the first case of recurrent radiation-associated leiomyosarcoma after invasive ductal carcinoma postoperative radiotherapy. A 45-year-old female patient with no familial history of cancer underwent breast-conserving surgery (BCS) and dissection of axillary lymph nodes for invasive ductal carcinoma in the right upper outer region in 2014. After the operation, she received adjuvant chemotherapy followed by radiotherapy and endocrine therapy. She came to our institution for a lump in the right breast radiation region in 2019. Ultrasonography revealed an oval mass, with ill-defined borders, irregular margins, and speculation at 6 o'clock direction of the right breast, approximately 2.5 cm from the nipple. Ultimately, excisional biopsy established the pathological diagnosis of leiomyosarcoma. The female then underwent simple mastectomy of the right breast. The patient received no radiation or chemotherapy after the simple mastectomy. After 13 months of follow-up, a lump was found in the right chest wall and axillary levels of the radiation region. Excisional biopsy confirmed the pathological diagnosis of leiomyosarcoma. This rare case indicated that simple mastectomy seemed to be inadequate treatment for radiation-associated leiomyosarcoma.
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http://dx.doi.org/10.21037/apm-20-2561DOI Listing
May 2021

Milky Layered Cerebrospinal Fluid in Intracranial Hemorrhage.

Eur Neurol 2021 May 27:1-2. Epub 2021 May 27.

Department of Neurocritical Care, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

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http://dx.doi.org/10.1159/000516259DOI Listing
May 2021

Glypican-3 and hepatocyte paraffin-1 combined with alpha-fetoprotein as a novel risk scoring model for predicting early recurrence of hepatocellular carcinoma after curative resection.

Eur J Gastroenterol Hepatol 2021 May 21. Epub 2021 May 21.

Organ Transplantation Center, The First Affiliated Hospital, Kunming Medical University, Kunming Department of Transplantation, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: At present, the predictive model of postsurgical recurrence for hepatocellular carcinoma (HCC) is not well-established. The aim of this study was to develop a novel model for prediction of postsurgical recurrence and survival for HCC.

Patients And Methods: Data from 112 patients who underwent curative liver resection from June 2014 to June 2017 in the First Affiliated Hospital of Kunming Medical University were collected retrospectively. Through the statistical analysis, we combined the results of glypican-3 (GPC3) and hepatocyte paraffin-1 (Heppar1) chemical staining in tumor tissues and preoperative alpha-fetoprotein (AFP) levels, and assigned risk scores to them, respectively, to establish an improved prognostic model for predicting recurrence in these patients.

Results: By univariate and multivariate analysis, AFP level [cut-off value: 382 ng/ml, area under the curve (AUC) = 0.652, 95% confidence interval (CI) = 0.539-0.765, P < 0.05] and GPC3/Heppar1 expression pattern from 10 putative prognostic factors were entered in risk factor scoring model to conjecture the tumor recurrence. At 36 months after liver resection, the recurrence rate of high-risk group in the novel risk scoring model reached 45.6%, which was significantly higher than that of low-risk group (9.1%). In this experiment, the AUC value of the model was 0.741 (95% CI = 0.644-0.839, P < 0.001), which was the highest among all the elements.

Conclusion: The novel risk scoring model of combing AFP cut-off value and GPC3/Heppar1 were shown to be effective at predicting early recurrence of HCC after curative resection.
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http://dx.doi.org/10.1097/MEG.0000000000002175DOI Listing
May 2021

Phase I clinical trial of EGFR-specific CAR-T cells generated by the piggyBac transposon system in advanced relapsed/refractory non-small cell lung cancer patients.

J Cancer Res Clin Oncol 2021 May 25. Epub 2021 May 25.

Department of Biotherapy, The Eastern Hepatobiliary Surgery Hospital, Shanghai, 201805, China.

Purpose: This phase I clinical trial is designed to assess the safety and feasibility of the epidermal growth factor receptor (EGFR) chimeric antigen receptor (CAR) T-cell generated by the piggyBac transposon system in advanced relapsed/refractory non-small cell lung cancer (NSCLC) patients. Compared to viral systems, the piggyBac transposon system is a simpler, more economical, and alternative way to introduce chimeric antigen receptor (CAR) transgenes into T cells.

Methods: This study recruited nine patients with advanced relapsed/refractory EGFR-positive NSCLC for two cycles of the piggyBac-generated EGFR-CAR T cells at dose of 1 × 10 cells/kg or 3 × 10 cells/kg of body weight. The patients were monitored for adverse events, clinical response, and persistence of plasma GFR-CAR T cells.

Results: Infusions of piggyBac-generated EGFR-CAR T cells were well tolerated in all nine patients. The most common adverse events were grade 1 to 3 fever and there were no patients who experienced grade 4 adverse events or serious cytokine release syndrome. After treatment, eight of nine patients showed detectable EGFR-CAR T cells in their peripheral blood. One patient showed a partial response and lasted for more than 13 months, while six had stable disease, and two had progressed disease. The progression-free survival of these nine patients was 7.13 months (95% CI 2.71-17.10 months), while the median overall survival was 15.63 months (95% CI 8.82-22.03 months).

Conclusion: This Phase I clinical trial revealed that the non-viral piggyBac transposon system-engineered EGFR-CAR T-cell therapy is feasible and safe in treatment of EGFR-positive advanced relapsed/refractory NSCLC patients. Future study will assess it in more patients or even possibly with a higher dose. Trial registration number NCT03182816.
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http://dx.doi.org/10.1007/s00432-021-03613-7DOI Listing
May 2021

The seed oil of ameliorates Aβ25-35-induced Alzheimer's disease in rats.

Food Sci Nutr 2021 May 30;9(5):2402-2413. Epub 2021 Mar 30.

TAAHC-SWU Medicinal Plant Joint R&D Centre Tibetan Collaborative Innovation Centre of Agricultural and Animal Husbandry Resources Food Science College Tibet Agricultural and Animal Husbandry University Nyingchi China.

, a plant of the Paeoniaceae family, has abundant genetic diversity in different populations, and the seed oil can be used in a diverse number of activities. However, its neuroprotective effect is not clear. We investigated the memory-improving effects and associated mechanisms of seed oil (PLSO) on amyloid beta (Aβ)25-35-induced Alzheimer's disease (AD) in rats. The Morris water maze test was undertaken, and subsequently, the content of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and acetylcholinesterase (ACHE) in the hippocampus was detected by biochemical analyses. To further study PLSO, we examined the pathologic structure and apoptosis of hippocampal tissue by staining. Immunohistochemical analysis was used to detect expression of IBA-1 and GFAP in the hippocampus. Detection of proinflammatory factors was achieved by reverse transcription-quantitative polymerase chain reaction and Western blotting. High-dose PLSO inhibited expression of GFAP and IBA-1. We demonstrated that high-dose PLSO can regulate activation of glial cells and mediate apoptosis of hippocampal cells, and significantly improve learning and memory deficits in AD rats. PLSO could be developed as a nutritional supplement and sold as a drug for AD prevention and/or treatment.
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http://dx.doi.org/10.1002/fsn3.2102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116862PMC
May 2021

Novel Truncating and Missense Variants in in Patients With Early-Onset Epilepsy.

Front Cell Dev Biol 2021 4;9:633819. Epub 2021 May 4.

Laboratory of Molecular Diagnosis, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Progressive myoclonic epilepsy (PME) is a rare neurodegenerative disease, characterized by myoclonic seizures and tonic clonic seizures, with genetical and phenotypical heterogeneity. The semaphorin 6B () gene has been recently reported a causal gene of PME. Independent studies are warranted to further support these findings. Here we report that one nonsense variant in NM_032108.3 exon17 c.2056C > T (p.Gln686) and one missense variant in exon14 c.1483G > T (p.Gly495Trp) of , both occurring , underlie early-onset epilepsy with variable severity and different response to treatment in two patients. analyses have demonstrated that the nonsense variant, p.Gln686, results in a truncated protein with remarkably increased expression compared to that of the wild type. The truncated protein presented more homogeneous and failed to locate in the plasma membrane. The missense variant p.Gly495Trp affects evolutionarily conserved amino acid and is located in the sema domain, a key functional domain of SEMA6B. It was predicted to perturb the SEMA6B function by altering the tertiary structure of mutant protein, although neither change of protein length and expression nor difference of cellular distribution was observed. Co-immunoprecipitation studies have demonstrated that both variants influence protein binding of SEMA6B and PlxnA2 with varying degrees. Our results provide further evidence to support the initial findings of being causal to epilepsy and indicate that mediating Semaphorin/Plexin signaling is the potential mechanism of the SEMA6B-related disease.
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http://dx.doi.org/10.3389/fcell.2021.633819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129541PMC
May 2021

Applications of the isolated-check visual evoked potential in primary open angle glaucoma with or without high myopia.

Int J Ophthalmol 2021 18;14(5):704-713. Epub 2021 May 18.

Department of Ophthalmology, Peking University First Hospital, Beijing 100034, China.

Aim: To explore the diagnostic performance of isolated-check visual evoked potential (icVEP) for primary open angle glaucoma (POAG) in both highly myopic and non-highly myopic populations and compare it with those of optical coherence tomography (OCT) and Heidelberg retinal tomography (HRT) parameters.

Methods: A total of 126 participants were recruited, including 31 highly myopic participants with POAG (HM-POAG), 36 non-highly myopic participants with POAG (NHM-POAG), 25 highly myopic participants without POAG (HM) and 34 controls without high myopia (Normal). All the participants underwent a complete ophthalmic examination. The signal-to-noise ratio (SNR) was used to assess the icVEP. Both qualitative and quantitative diagnostic performances of OCT, HRT and the icVEP were analyzed and compared.

Results: Based on the criterion of SNR≤1, the diagnostic performance of the icVEP in highly myopic subjects [area under the receiver operating characteristic curve (AUC)=0.862] was better than that in non-highly myopic subjects (AUC=0.789), and the SNR had fairly good specificity. In distinguishing the HM-POAG and HM groups, the AUC of the SNR was not different from those of the OCT and HRT parameters (>0.05) in either the qualitative or quantitative comparison. In the qualitative analysis, the icVEP showed good consistency with damage to the central 10° of the visual field (kappa=0.695-0.747, <0.001).

Conclusion: The icVEP has the potential to discriminate individuals with and without POAG, especially in populations with high myopia.
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http://dx.doi.org/10.18240/ijo.2021.05.10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077015PMC
May 2021

[Effect of fire needling on mild to moderate knee osteoarthritis and related serum inflammatory cytokines].

Zhongguo Zhen Jiu 2021 May;41(5):493-7

Department of Acupuncture and Moxibustion, Beijing Hospital of TCM, Capital Medical University, Beijing 100010, China;Beijing Key Laboratory of Acupuncture Neuromodulation, Beijing Hospital of TCM, Capital Medical University, Beijing 100010, China.

Objective: To compare the clinical effect of fire needling and filiform needling for mild to moderate knee osteoarthritis (KOA) and observe the influence on related serum inflammatory sytokines.

Methods: A total of 60 patients with mild to moderate KOA were randomly divided into an observation group (30 cases, 4 cases dropped off) and a control group (30 cases, 4 cases dropped off). Both groups were given basic health management, and the acupoints of the two groups were Liangqiu (ST 34), Xuehai (SP 10), Neixiyan (EX-LE 4), Dubi (ST 35), Yanglingquan (GB 34) and point. The observation group was treated with fire needling, while the control group was treated with filiform needling. Both groups were treated once every other day, 3 times a week for 2 weeks. The Western Ontario and McMaster University osteoarthritis index (WOAMC) scores of the two groups were compared before treatment, in 1, 2 weeks of treatment and 2, 6 weeks of follow-up. ELISA method was used to detect the levels of serum interleukin 1α (IL-1α), tumor necrosis factor α (TNF-α) and matrix metalloproteinase 3 (MMP-3) before treatment and in 2 weeks of treatment. The clinical effect of the two groups was evaluated in 1, 2 weeks of treatment and 2, 6 weeks of follow-up.

Results: At each time point of treatment and follow-up, the pain, stiffness, difficulty of daily activities scores and WOMAC total scores of the two groups were lower than those before treatment (<0.05); the stiffness score of the observation group was lower than that of the control group in 1 week of treatment (<0.05); the various scores and total scores of the WOMAC scale of the observation group were lower than those of the control group in 1, 2 weeks of treatment and 2, 6 weeks of follow-up (<0.05). In 2 weeks of treatment, the levels of serum MMP-3 and IL-1α in the observation group and IL-1α in the control group were higher than those before treatment (<0.05). In 1 week of treatment and 2, 6 weeks of follow-up, the total effective rates of the observation group were higher than those in the control group (<0.05).

Conclusion: Fire needling can improve the pain, stiffness and joint dysfunction of patients with mild to moderate KOA, and increase serum MMP-3 and IL-1α levels. Its short and long term clinical effects are better than filiform needling.
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http://dx.doi.org/10.13703/j.0255-2930.20200708-k0003DOI Listing
May 2021

The clinical significance and function of miR-146 in the promotion of epidural fibrosis.

Genet Mol Biol 2021 14;44(2):e20200447. Epub 2021 May 14.

The Affiliated Hospital of Qingdao University, Department of Spinal Surgery, Qingdao, Shandong, China.

Epidural fibrosis is the main cause of failed back surgery syndrome. To investigate the role of miR-146 in the diagnosis and development of epidural fibrosis. Lumbar disc tissues were collected from 72 lumbar disc herniation patients (45 developed epidural fibrosis and 27 did not). The expression of miR-146 in collected tissues and isolated epidural fibroblasts was detected by RT-qPCR. The relative levels of pro-inflammatory cytokines were analyzed by ELISA. The effect of miR-146 on the proliferation of fibroblasts was evaluated by MTT assay. miR-146 was significantly upregulated in epidural fibrosis patients compared with control patients. The expression of miR-146 was closely associated with the location, lower limb symptom and duration of disease of epidural fibrosis patients, and was positively correlated with the relative levels of pro-inflammatory cytokines. Moreover, miR-146 could discriminate epidural fibrosis patients from control patients. In isolated epidural fibroblasts, the overexpression of miR-146 dramatically enhanced its proliferation and the inflammatory response. miR-146 serves as a diagnostic biomarker for the early detection of epidural fibrosis. The upregulation of miR-146 enhanced the fibroblasts proliferation and inflammatory response in epidural fibrosis. This study provides a novel potential therapeutic target for epidural fibrosis.
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http://dx.doi.org/10.1590/1678-4685-GMB-2020-0447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127721PMC
May 2021

Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell.

Reprod Domest Anim 2021 May 15. Epub 2021 May 15.

College of Life Science, Northeast Agricultural University, Harbin, China.

In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse-porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig-origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells' potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization.
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http://dx.doi.org/10.1111/rda.13952DOI Listing
May 2021

Yu-Ping-Feng Formula Exerts Antilung Cancer Effects by Remodeling the Tumor Microenvironment through Regulating Myeloid-Derived Suppressor Cells.

Evid Based Complement Alternat Med 2021 20;2021:6624461. Epub 2021 Apr 20.

Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.

Yu-Ping-Feng (YPF) formula is a classical prescription used for enhancing the body's immunity function in traditional Chinese medicine (TCM). In clinical practice, the YPF formula has been reported to exhibit antilung cancer and immunomodulatory effect. However, the relationship between them remains unclear. The present study aimed to investigate the antilung cancer effect of the YPF formula and its immune-related mechanisms. The C57BL/6 tumor-bearing mice model was established and randomly divided into the YPF group and the control group. Tumor volume, spleen weight, and survival in both groups were measured and evaluated during 28 days of consecutive intervention. Flow cytometry was used to detect the proportion of immune cell subsets. Myeloid-derived suppressor cells (MDSCs) were induced in vitro from bone marrow cells. After intervention by the YPF formula, CCK-8 and flow cytometry analyses were performed to detect proliferation and apoptosis of MDSCs. A coculture system containing T cells and MDSCs was established to further study the role of MDSCs in the regulation of T-cell subsets proportion by the YPF formula. The expressions of MDSCs-related genes and proteins were detected by RT-PCR and Western blotting. The results showed the YPF formula inhibited tumor growth, reduced spleen weight, and prolonged the survival of mice. Besides, the proportions of MDSCs subsets and Regulatory (Treg) in the YPF group decreased, whereas those of CDT and CDT increased both in vitro and in vivo. CCK-8 and flow cytometry demonstrated that the YPF formula could inhibit proliferation and promote apoptosis of MDSCs. The coculture experiments further confirmed that MDSCs served a critical role in regulating the tumor microenvironment by the YPF formula. RT-PCR and Western blotting indicated that the levels of MDSCs' activation and proliferation-related proteins and genes were downregulated in the YPF group. Therefore, our results demonstrated that the YPF formula could promote apoptosis and inhibit the proliferation of MDSCs. As a result, the negative regulatory effect on the positive immune cells induced by MDSCs was weakened, thus achieving the antilung cancer effect by remodeling the tumor microenvironment.
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http://dx.doi.org/10.1155/2021/6624461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079197PMC
April 2021

Prediction of Synthetic Lethal Interactions in Human Cancers using Multi-view Graph Auto-Encoder.

IEEE J Biomed Health Inform 2021 May 11;PP. Epub 2021 May 11.

Synthetic lethality (SL) is a very important concept for the development of targeted anticancer drugs. However, experimental methods for SL detection often suffer from various issues like high cost and low consistency across cell lines. Hence, computational methods for predicting novel SLs have recently emerged as complements for wet-lab experiments. In addition, SL data can be represented as a graph where nodes are genes and edges are the SL interactions. It is thus motivated to design advanced graph-based machine learning algorithms for SL prediction. In this paper, we propose a novel SL prediction method using Multi-view Graph Auto-Encoder (SLMGAE). We consider the SL graph as main view and the graphs from other data sources (e.g., PPI, GO, etc.) as support views. Multiple Graph Auto-Encoders (GAEs) are implemented to reconstruct the graphs for different views. We further design an attention mechanism, which assigns the weights for support views, to combine all the reconstructed graphs for SL prediction. The overall SLMGAE model is then trained by minimizing both the reconstruction error and prediction error. Experimental results on the SynLethDB dataset show that SLMGAE outperforms state-of-the-arts. The case studies on novel predicted SLs also illustrate the effectiveness of our SLMGAE method. In addition, the source codes, data and supplementary materials for our SLMGAE are available via https://github.com/DiNg1011/SLMGAE.
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http://dx.doi.org/10.1109/JBHI.2021.3079302DOI Listing
May 2021

Development of a -based counterselectable system for targeted gene deletion in .

J Zhejiang Univ Sci B 2021 May;22(5):383-396

School of Life Sciences, North China University of Science and Technology, Tangshan 063210, China.

produces many valuable and important biomolecules with clinical and pharmaceutical applications. The development of simple and highly efficient gene editing tools for genetic modification of is highly desirable. In this study, we developed a screening system for targeted gene knockout using a uracil auxotrophic host (Δ) resistant to the highly toxic uracil analog of 5-fluoroorotic acid (5-FOA) converted by PyrF, and a non-replicative vector pKC1132-pyrF carrying the complemented gene coding for orotidine-5'-phosphate decarboxylase. The gene acts as a positive selection and counterselection marker for recombinants during genetic modifications. Single-crossover homologous integration mutants were selected on minimal medium without uracil by reintroducing along with pKC1132-pyrF into the genome of the mutant Δ at the targeted locus. Double-crossover recombinants were generated, from which the gene, plasmid backbone, and targeted gene were excised through homologous recombination exchange. These recombinants were rapidly screened by the counterselection agent, 5-FOA. We demonstrated the feasibility and advantage of using this based screening system through deleting the gene, which encodes the cluster-situated regulator that directly activates oxytetracycline biosynthesis in M4018. This system provides a new genetic tool for investigating the genetic characteristics of species.
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http://dx.doi.org/10.1631/jzus.B2000606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110465PMC
May 2021

Exploiting a Multiphase Pure Formamidinium Lead Perovskite for Efficient Green-Light-Emitting Diodes.

ACS Appl Mater Interfaces 2021 May 10;13(19):23067-23073. Epub 2021 May 10.

Key Laboratory for Physical Electronics and Devices of the Ministry of Education & Shaanxi Key Lab of Information Photonic Technique, School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Formamidinium (FA)-based perovskites have demonstrated excellent advances in optoelectronics recently, but in terms of perovskite compounds, the optimal condition of their composition optimization remains controversial. Herein, we boosted the performance of perovskite light-emitting diodes (PeLEDs) in both efficiency and stability through composition and phase engineering. The low-dimensional FAPbBr particles emerged and mixed with the FAPbBr composites when the FABr content is excessive. By regulating the phase composition in multiphase FAPbBr perovskites, the maximum external quantum efficiency (EQE) of optimal PeLEDs (5.14%) is 2.7-fold higher than that of pure FAPbBr-based PeLEDs (1.90%), and device stability is improved. Further optimizing the film quality and emission efficiency with Rb ions, the EQE of PeLEDs can be increased to 6.01%, with a half-lifetime of about 185 s under the high current density. This strategy of phase composition optimization in FA-based perovskites provides an effective way to process high-efficiency and stable PeLEDs.
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http://dx.doi.org/10.1021/acsami.1c00116DOI Listing
May 2021

Salt glands play a pivotal role in the salt resistance of four recretohalophyte Limonium Mill. species.

Plant Biol (Stuttg) 2021 May 10. Epub 2021 May 10.

Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Ji'nan Shandong, 250014, P.R. China.

Limonium Mill. plants are typical recretohalophytes, as they withstand salt stress by secreting excess salt onto the leaf surface through salt glands. However, it's known little on the salinity thresholds of these plants and function of their salt glands in salt tolerance. Here, we investigated the salinity thresholds of salt tolerance of Limonium species L. aureum (Linn.) Hill, L. gmelinii (Willd.) Kuntze, L. otolepis (Schrenk) Kuntze, and L. sinuatum (L.) Mill under various concentrations of NaCl. The salinity thresholds of L. otolepis, L. aureum, L. sinuatum, and L. gmelinii were 300, 350, 400, and 420 mM NaCl, respectively. Correlation analysis indicated that total dry weight, chlorophyll content, and intercellular CO concentration were highly positively correlated with the total fresh weights of all four Limonium species and could therefore be used as indicators of plant salt tolerance. Furthermore, as the salt gland density on the leaf surface increased, the rate of salt secretion per salt gland increased, allowing more Na to be secreted from the plant. Redundancy discriminant analysis indicated that salt gland density, Na content, and Na secretion rate per salt gland were positively correlated with salt concentration. These observations support the notion that salt glands play important roles in the adaptation of Limonium species to high-salinity conditions.
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http://dx.doi.org/10.1111/plb.13284DOI Listing
May 2021

Effect of short-term intake of four sweeteners on feed intake, solution consumption and neurotransmitters release on mice.

J Food Sci Technol 2021 Jun 19;58(6):2227-2236. Epub 2020 Aug 19.

Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070 China.

This study focused on the effect of short-term intake of sweeteners on feed intake, solution consumption and neurotransmitters release on mice. The results showed that the free drinking of 10 mM sucralose solution, 100 mM maltose solution, 3 mM saccharin solution and 3 g/L stevioside solution for 32 days will not affect the normal development of the body weight and feed intake of the mice. The consumption of maltose solution was significantly higher than that of the other sweeteners. The leptin and insulin levels increased significantly after the short-term intake of these four sweeteners. The dopamine (DA) content in the whole brain of the mice increased significantly only in the maltose group. These results indicate that the short-term intake of the preferred concentrations of maltose, stevioside, sucralose and saccharin will not affect the body weight and feed intake of the mice. Mice prefer maltose solution to other sweeteners solutions. The 100 mM maltose solution and 3 mM saccharin solution could result in the oxidative stress on mice after 32 days' short-term intake. Compared with other sweeteners, only sugars that could be broken down into small molecules of glucose might have a positive effect on dopamine levels.
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http://dx.doi.org/10.1007/s13197-020-04733-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076381PMC
June 2021

The Effects of M1/M2 Macrophages on the mRNA Expression Profile of Diabetic Glomerular Endothelial Cells.

Nephron 2021 May 6:1-11. Epub 2021 May 6.

Department of Nephrology, the Second Xiangya Hospital of Central South University, Changsha, China.

Objectives: The aim of this study was to explore the effects of M1/M2 macrophages on the mRNA expression profile of glomerular endothelial cells (GECs) treated with advanced glycosylation end products (AGEs).

Methods: We cocultured M1/M2 macrophages and GECs treated with AGEs. The mRNA expression profile was detected by transcriptome sequencing. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was performed using the online tool Database for Annotation, Visualization, and Integrated Discovery (DAVID). The expression of differential genes was confirmed using droplet digital PCR and Western blot.

Results: The results showed that 1,337 genes were significantly changed in M2 cocultured with AGE-treated GECs compared to those of the M1 cocultured with AGE-treated GECs. The KEGG analysis results show that the differentially expressed genes are primarily involved in specific pathways, including the rat sarcoma signaling pathway and the mitogen-activated protein kinase (MAPK) signaling pathway. The expression of Braf, Fgfr2, Map3k1, and Mras, which are involved in the MAPK signaling pathway, was downregulated in cocultured M2 with AGE-treated GECs and was consistent with sequencing. Therefore, the cocultured M1/M2 macrophages with AGE-treated GECs can affect the expression of mRNA in GECs, and M2 macrophages via the MAPK signaling pathway may protect GECs from damage by AGEs.

Conclusion: Coculturing M1/M2 macrophages and GECs treated with AGEs can affect the mRNA expression in GECs. M2 macrophages can inhibit the Braf, Fgfr2, Map3k1, Mras, and p-ERK expression in the MAPK signaling pathway, which may be related to M2 macrophages protecting GECs from damage by AGEs.
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http://dx.doi.org/10.1159/000513268DOI Listing
May 2021

Global, regional and national burden of anxiety disorders from 1990 to 2019: results from the Global Burden of Disease Study 2019.

Epidemiol Psychiatr Sci 2021 May 6;30:e36. Epub 2021 May 6.

Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, China.

Aims: Anxiety disorders are widespread across the world. A systematic understanding of the disease burden, temporal trend and risk factors of anxiety disorders provides the essential foundation for targeted public policies on mental health at the national, regional, and global levels.

Methods: The estimation of anxiety disorders in the Global Burden of Disease Study 2019 using systematic review was conducted to describe incidence, prevalence and disability-adjusted life years (DALYs) in 204 countries and regions from 1990 to 2019. We calculated the estimated annual percentage change (EAPC) to quantify the temporal trends in anxiety disorders burden by sex, region and age over the past 30 years and analysed the impact of epidemiological and demographic changes on anxiety disorders.

Results: Globally, 45.82 [95% uncertainty interval (UI): 37.14, 55.62] million incident cases of anxiety disorders, 301.39 million (95% UI: 252.63, 356.00) prevalent cases and 28.68 (95% UI: 19.86, 39.32) million DALYs were estimated in 2019. Although the overall age-standardised burden rate of anxiety disorders remained stable over the past three decades, the latest absolute number of anxiety disorders increased by 50% from 1990. We observed huge disparities in both age-standardised burden rate and changing trend of anxiety disorders in sex, country and age. In 2019, 7.07% of the global DALYs due to anxiety disorders were attributable to bullying victimisation, mainly among the population aged 5-39 years, and the proportion increased in almost all countries and territories compared with 1990.

Conclusion: Anxiety disorder is still the most common mental illness in the world and has a striking impact on the global burden of disease. Controlling potential risk factors, such as bullying, establishing effective mental health knowledge dissemination and diversifying intervention strategies adapted to specific characteristics will reduce the burden of anxiety disorders.
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http://dx.doi.org/10.1017/S2045796021000275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157816PMC
May 2021

Bandwidth-control orbital-selective delocalization of 4f electrons in epitaxial Ce films.

Nat Commun 2021 May 4;12(1):2520. Epub 2021 May 4.

Center for Correlated Matter and Department of Physics, Zhejiang University, Hangzhou, China.

The 4f-electron delocalization plays a key role in the low-temperature properties of rare-earth metals and intermetallics, and it is normally realized by the Kondo coupling between 4f and conduction electrons. Due to the large Coulomb repulsion of 4f electrons, the bandwidth-control Mott-type delocalization, commonly observed in d-electron systems, is difficult in 4f-electron systems and remains elusive in spectroscopic experiments. Here we demonstrate that the bandwidth-control orbital-selective delocalization of 4f electrons can be realized in epitaxial Ce films by thermal annealing, which results in a metastable surface phase with reduced layer spacing. The quasiparticle bands exhibit large dispersion with exclusive 4f character near [Formula: see text] and extend reasonably far below the Fermi energy, which can be explained from the Mott physics. The experimental quasiparticle dispersion agrees well with density-functional theory calculation and also exhibits unusual temperature dependence, which could arise from the delicate interplay between the bandwidth-control Mott physics and the coexisting Kondo hybridization. Our work opens up the opportunity to study the interaction between two well-known localization-delocalization mechanisms in correlation physics, i.e., Kondo vs Mott, which can be important for a fundamental understanding of 4f-electron systems.
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http://dx.doi.org/10.1038/s41467-021-22710-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096960PMC
May 2021

Facilitators and Barriers to Take Up Clinician-Collected and Self-Collected HPV Tests among Chinese Men Who Have Sex with Men.

Viruses 2021 04 19;13(4). Epub 2021 Apr 19.

Faculty of Medicine, JC School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, China.

Regular tests for human papillomavirus (HPV) and early treatment could represent an important strategy for preventing anal cancers among men who have sex with men (MSM). This study investigated facilitators and barriers to take up clinician-collected and self-collected HPV tests among Chinese MSM. This study was based on the baseline sample of a longitudinal study promoting HPV vaccination among 350 Chinese MSM who had never received an HPV vaccination. The baseline survey was conducted from August 2019 to April 2020. The prevalence of any HPV tests uptake in lifetime was 19.1%; 4.9% had HPV infection in anus, genital, oral cavity, and other places. Among the participants, 20% and 76.8% intended to take up self-financed and free clinician-collected HPV tests, and 76.8% intended to use free self-collected HPV tests. After adjusting for significant background characteristics, perceived risk of HPV infection, and perceived benefits, barriers, cue to action, and self-efficacy related to HPV tests in general and/or specific to self-collected HPV tests were associated with behavioral intention to take up free clinician-collected and/or self-collected HPV tests. Less than 20% of Chinese MSM reported HPV tests uptake. Modifying perceptions related to HPV tests may be useful to increase HPV tests coverage in this group.
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http://dx.doi.org/10.3390/v13040705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073020PMC
April 2021