Publications by authors named "Yuan Deng"

160 Publications

A novel DNA-methylation-based model that effectively predicts prognosis in hepatocellular carcinoma.

Biosci Rep 2021 Feb 26. Epub 2021 Feb 26.

Gansu Provincial Hospital, Lanzhou, China.

Purpose: To build a novel predictive model for hepatocellular carcinoma (HCC) patients based on DNA methylation data.

Methods: Four independent DNA methylation datasets for HCC were used to screen for common differentially methylated genes (CDMGs). Gene Ontology (GO) enrichment, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to explore the biological roles of CDMGs in HCC. Univariate Cox analysis and LASSO Cox analysis were performed to identify survival-related CDMGs (SR-CDMGs) and to build a predictive model. The importance of this model was assessed using Cox regression analysis, propensity score-matched (PSM) analysis and stratification analysis. A validation group from the Cancer Genome Atlas was constructed to further validate the model.

Results: Four SR-CDMGs were identified and used to build the predictive model. The risk score of this model was calculated as follows: risk score = (0.01489826 × methylation level of WDR69) + (0.15868618 × methylation level of HOXB4) + (0.16674959 × methylation level of CDKL2) + (0.16689301 × methylation level of HOXA10). Kaplan-Meier analysis demonstrated that patients in the low-risk group had a significantly longer overall survival (log-rank P-value = 0.00071). The Cox model multivariate analysis and PSM analysis identified the risk score as an independent prognostic factor (P < 0.05). Stratified analysis results further confirmed this model performed well. By analyzing the validation group, the results of receiver operating characteristic curve analysis and survival analysis further validated this model.

Conclusion: Our DNA-methylation-based prognosis predictive model is effective and reliable in predicting prognosis for patients with HCC.
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http://dx.doi.org/10.1042/BSR20203945DOI Listing
February 2021

Successful treatment of two relapsed patients with t(11;19)(q23;p13) acute myeloid leukemia by CLAE chemotherapy sequential with allogeneic hematopoietic stem cell transplantation: Case reports.

Oncol Lett 2021 Mar 6;21(3):178. Epub 2021 Jan 6.

Department of Hematology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

The prognosis of patients with relapsed/refractory acute myeloid leukemia (R/R AML) is poor, with a 3-year overall survival rate of 10%. Patients with translocation (t)(11;19)(q23;p13) have a higher risk of relapse and there is no optimal regimen for these patients. The present study treated two young patients with t(11;19)(q23;p13) AML, who relapsed after one or two cycles of consolidation, with a salvage treatment consisting of sequential cladribine, cytarabine and etoposide (CLAE) and allogeneic hematopoietic stem cell transplantation (allo-HSCT). Both neutrophil and platelet engraftments were achieved within 15 days, and no severe transplant-related complications and graft-versus-host diseases were observed. Following allo-HSCT, both patients achieved complete hematologic and cytogenetic remission. Decitabine was used for the prophylaxis of relapse. The two patients remained alive and disease-free for 100 days following allo-HSCT. The results presented here suggest that CLAE regimen sequential with allo-HSCT may be effective in treating patients with R/R AML, with t(11;19)(q23;p13). However, further studies and a larger sample size are required to validate the effectiveness of this treatment regimen.
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http://dx.doi.org/10.3892/ol.2021.12439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816337PMC
March 2021

vNOTES Hysterectomy with Sentinel Lymph Node Mapping for Endometrial Cancer: Description of Technique and Perioperative Outcomes.

J Minim Invasive Gynecol 2021 Jan 29. Epub 2021 Jan 29.

Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University, Chongqing, China (all authors). Electronic address:

Study Objective: To explore the technique and clinical value of transvaginal natural orifice transluminal endoscopic surgery (vNOTES) in hysterectomy and sentinel lymph node (SLN) mapping for endometrial cancer by comparing its perioperative outcomes with those of laparoscopic staging.

Design: Retrospective cohort study.

Setting: Department of gynecology at a tertiary medical center.

Patients: All women diagnosed with endometrial cancer who underwent minimally invasive surgery at our center between August 2017 and May 2020.

Interventions: Both vNOTES and laparoscopic approaches were used for hysterectomy and SLN mapping. The success of SLN detection as well as perioperative outcomes were subsequently analyzed.

Measurements And Main Results: This study included 74 patients; 23 patients underwent vNOTES surgery, whereas 51 underwent standard laparoscopic surgery. The total successful SLN detection was 95.7% in the vNOTES group and 92.2% in the laparoscopy group (p >.05), whereas the bilateral success rates were 87.0% and 90.2%, respectively. No difference in SLN detection was observed between the 2 groups in terms of the side-specific mapping efficacy quotient (91.3% vs 91.2%, p = .47). The number of harvested SLNs, operative time, estimated blood loss, and intraoperative complications in the 2 groups were similar. One (4.3%) postoperative complication occurred in the vNOTES group vs 4 (7.9%) in the laparoscopy group (p = .029), and the median postoperative hospital stay was 3 days vs 4 days (p = .003).

Conclusion: This study suggests that the vNOTES procedure is feasible, with a potentially decreased postoperative hospital stay, faster recovery, and better cosmetic results. However, prospective research is needed to validate its broader clinical application.
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http://dx.doi.org/10.1016/j.jmig.2021.01.022DOI Listing
January 2021

Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

J Biol Chem 2020 Dec 3:100121. Epub 2020 Dec 3.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, China;. Electronic address:

Cancer stem-like cells (CSCs) contribute to the high rate of tumor heterogeneity, metastasis, therapeutic resistance, and recurrence. Histone lysine demethylase 4D (KDM4D or JMJD2D) is highly expressed in colon and liver tumors, where it promotes cancer progression; however, the role of JMJD2D in CSCs remains unclear. Here, we show that JMJD2D expression was increased in liver cancer stem-like cells (LCSCs); downregulation of JMJD2D inhibited the self-renewal of LCSCs in vitro and in vivo and inhibited the lung metastasis of LCSCs by reducing the survival and the early lung seeding of circulating LCSCs. Mechanistically, JMJD2D promoted LCSC self-renewal by enhancing the expression of CSC markers EpCAM and Sox9; JMJD2D reduced H3K9me3 levels on the promoters of EpCAM and Sox9 to enhance their transcription via interaction with β-catenin/TCF4 and Notch1 intracellular domain, respectively. Restoration of EpCAM and Sox9 expression in JMJD2D-knockdown liver cancer cells rescued the self-renewal of LCSCs. Pharmacological inhibition of JMJD2D using 5-c-8HQ reduced the self-renewal of LCSCs and liver cancer progression. Collectively, our findings suggest that JMJD2D promotes LCSC self-renewal by enhancing EpCAM and Sox9 expression via Wnt/β-catenin and Notch signaling pathways and is a potential therapeutic target for liver cancer.
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http://dx.doi.org/10.1074/jbc.RA120.015335DOI Listing
December 2020

Re: "Loss of Stimulated Tear Secretion After Orbital Reconstruction".

Ophthalmic Plast Reconstr Surg 2021 Jan-Feb 01;37(1):92-94

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http://dx.doi.org/10.1097/IOP.0000000000001878DOI Listing
January 2021

Screening Study on the Anti-Angiogenic Effects of Traditional Chinese Medicine - Part II: .

J Cancer 2021 1;12(1):124-133. Epub 2021 Jan 1.

Cancer Research Institute, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China.

Part 2 of our ongoing research with anti-angiogenic effects focuses on a Traditional Chinese Medicine (TCM). We screened six TCM and noticed that has a potent anti-angiogenic effect in zebrafish. This study aims to determine the genetic mechanisms underlying the anti-angiogenic effects of . was decocted, concentrated, sieved and desiccated to attain the water extract. 200μg/mL water extract (WCWE) was diluted in 0.1% dimethyl sulfoxide (DMSO) and given to zebrafish via fish water. 48h post-fertilization (hpf) transgenic zebrafish were used to assay angiogenesis. mRNA-seq, qRT-PCR assay and a parallel reaction monitor (PRM) were carried out to reveal the underlying mechanisms. WCWE showed a significant anti-angiogenic effect in zebrafish. The results of mRNA-seq showed that there were 1119 genes up-regulated and 1332 genes down-regulated by WCWE. The bioinformatic analysis based on mRNA-seq demonstrated that the proteasome signaling pathway was significantly down-regulated. The results of the qRT-PCR assay were consistent with those of the mRNA-seq assay. The results of the PRM assay showed that nine proteins involved in proteasome signaling and the protein expression level of ctnnb2 were significantly down-regulated. The results of the KEGG pathway analysis based on PRM assay demonstrated that WCWE may have an inhibitory action on the regulatory particle of the proteasome. has a significant anti-angiogenic effect in zebrafish and it may have an inhibitory action on the regulatory particle of the proteasome. The mechanisms underlying the anti-angiogenic effects of may be related to the down-regulation of proteasome/β-catenin signaling in zebrafish.
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http://dx.doi.org/10.7150/jca.52971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738828PMC
January 2021

[Significance of Targeted Sequencing Assay for Patients with Suspected Myeloid Malignancies].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Dec;28(6):1985-1990

Department of Hematology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Key Laboratory of Hematology of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China E-mail:

Objective: To investigate the clinical significance of the targeted next-generation sequencing assay for patients with suspected myeloid malignancies.

Methods: A total of 39 hematopenia patients with suspected myeloid malignamies in Department of Hematology of The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University from January 2018 to April 2019 were treated, 20 hot spot genes of myelodysplastic syndrome (MDS) were detected.

Results: Regarding the diagnostic type, there were 7 cases of idiopathic cytopenia of undetermined significance (ICUS), 8 cases of clonal cytopenias of undetermined significance (CCUS) and 24 cases of myeloid myeloid malignancies which included 18 cases of MDS, 4 cases of myelodysplastic/myeloproliferative neoplasms (MDS/MPN) and 2 cases of acute myeloid leukemia. Positive mutation was detected in 70.8% (17/24) of myeloid malignancy patients , and 72.7% (16/22) in MDS and MDS/MPN patients. The main mutation types were ASXL1, TET2 and RUNX1. Compared with gene negative group, there were no significant differences in sex, age (<60 years old or ≥60 years old), proportion of bone marrow blast cells (<5% or≥5%) and cytogenetics (good, medium and poor) (P>0.05). Furthermore, all 8 CCUS patients showed positive mutation, and the incidence of double or multiple mutation in CCUS group was significantly lower than that of the MDS and MDS/MPN group (37.5% vs 54.5%) (P=0.002). The mutation types between the two groups were similar, and there was no significant difference in variant allele frequency (P>0.05).

Conclusion: Our results suggest that there are high rates of double or multiple mutations in myeloid malignancies, especially in patients with MDS and MDS/MPN. Targeted sequencing assay can improve the diagnosis of myeloid malignancies, and guide clinical treatment.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.06.031DOI Listing
December 2020

Imaging Cellular Aerobic Glycolysis using Carbon Dots for Early Warning of Tumorigenesis.

Adv Mater 2021 Jan 27;33(1):e2005096. Epub 2020 Nov 27.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China.

Early warning of tumor formation is crucial for the classification, treatment, and prognosis of tumor patients. Here, a new strategy is reported, aimed at realizing this goal based on imaging aerobic glycolysis processes using nitrogen-doped carbon dots (N-CDs) as fluorescent probes. The intensity of the photoluminescence emitted by the N-CDs is specifically enhanced by nicotinamide adenine dinucleotide (NAD , oxidized) in the physiological environment. The N-CDs allow a few (five to ten) abnormal cells in spontaneous hepatocellular carcinoma models to be identified before the in situ development of tumor tissue. The N-CD probes can also distinguish tumor cells from normal cells and be used to evaluate their proliferation activity (with a specificity of up to 96.15% in 13 types of tumor cells and 90.90% in orthotopic xenograft models). The N-CDs are successfully used to monitor the invasion of tumor cells into neighboring tissues and body fluids in 49 clinical samples (with a sensitivity up to 79.31%). These included three vitreous body samples (from patients with retinoblastoma), 42 urine samples (22 patients clinically diagnosed with urothelium carcinoma and 20 healthy persons), and four hydrothorax samples (from patients with metastatic lesions).
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http://dx.doi.org/10.1002/adma.202005096DOI Listing
January 2021

A custom-made conformer wrapped in lower oral mucosa for the correction of severely contracted socket.

Eur J Ophthalmol 2020 Nov 25:1120672120974940. Epub 2020 Nov 25.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: To investigate the clinical effects of a custom-made conformer wrapped in lower oral mucosa for the correction of severely contracted socket.

Methods: Clinical data and photographs of 32 patients with severely contracted socket were retrospectively analyzed. Among the 32 cases, 21 were males and 11 were females. Their ages ranged from 20 to 71 years (mean, 45 years). Each patient underwent conjunctival sac plasty using a custom-made conformer wrapped by oral mucosa, then compression bandage for 1 month. All patients received tarsorrhaphy 6 months postoperatively, and an artificial eye was worn 1 week later.

Results: All the grafts were fully vascularized. The conjunctival sacs had enough space to fit the desirable artificial eyes, and adequate aesthetic outcomes were reached postoperatively. The mean depth of the upper fornix was 4.28 ± 0.66 mm preoperatively and 18.84 ± 0.65 mm postoperatively ( < 0.01), and lower fornix depth was 2.69 ± 0.42 and 8.78 ± 0.82 mm, respectively ( < 0.01). Lower lip deformity occurred in one case.

Conclusion: A custom-made conformer wrapped in lower oral mucosa was a safe and effective grafting material. The grafts can be effectively used in the reconstruction of severely contracted socket and lead to good cosmetic outcomes.
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http://dx.doi.org/10.1177/1120672120974940DOI Listing
November 2020

Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

J Biol Chem 2020 Nov 24. Epub 2020 Nov 24.

School of Life Sciences, Xiamen University, China.

Cancer stem-like cells (CSCs) contribute to the high rate of tumor heterogeneity, metastasis, therapeutic resistance, and recurrence. Histone lysine demethylase 4D (KDM4D or JMJD2D) is highly expressed in colon and liver tumors, where it promotes cancer progression; however, the role of JMJD2D in CSCs remains unclear. Here, we show that JMJD2D expression was increased in liver cancer stem-like cells (LCSCs); downregulation of JMJD2D inhibited the self-renewal of LCSCs in vitro and in vivo and inhibited the lung metastasis of LCSCs by reducing the survival and the early lung seeding of circulating LCSCs. Mechanistically, JMJD2D promoted LCSC self-renewal by enhancing the expression of CSC markers EpCAM and Sox9; JMJD2D reduced H3K9me3 levels on the promoters of EpCAM and Sox9 to enhance their transcription via interaction with β-catenin/TCF4 and Notch1 intracellular domain, respectively. Restoration of EpCAM and Sox9 expression in JMJD2D-knockdown liver cancer cells rescued the self-renewal of LCSCs. Pharmacological inhibition of JMJD2D using 5-c-8HQ reduced the self-renewal of LCSCs and liver cancer progression. Collectively, our findings suggest that JMJD2D promotes LCSC self-renewal by enhancing EpCAM and Sox9 expression via Wnt/β-catenin and Notch signaling pathways and is a potential therapeutic target for liver cancer.
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http://dx.doi.org/10.1074/jbc.RA120.015335DOI Listing
November 2020

Application of da Vinci robot with the"3 + 2" mode in radical gastrectomy for gastric cancer.

Medicine (Baltimore) 2020 Nov;99(46):e22988

Department of General Surgery.

The aim of this study is to investigate the feasibility and advantages of the da Vinci robotic system with the "3 + 2" mode (3 robotic arms and 2 assistants) in radical gastrectomy for gastric cancer.The clinical data of 65 patients who underwent da Vinci robotic gastrectomy with the "3 + 2" mode from July 2016 to October 2019 were grouped into an observation group. An additional 65 patients who underwent robotic gastrectomy under the classic mode during the same period were grouped into a control group. The short-term surgical outcomes were compared between 2 different groups.Compared with the control group, the observation group had a significantly shorter operative time (176.18 ± 15.49 vs 203.85 ± 12.77 minutes, P < .001) and lower operation costs ($2761.19 ± $191.91 vs $3690.91 ± $162.82; P < .001). No statistical differences in other outcomes were observed (P > .05).We show that robotic gastrectomy with "3 + 2" mode is a safe and beneficial surgical procedure in new robotic surgery institutions.
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http://dx.doi.org/10.1097/MD.0000000000022988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668502PMC
November 2020

Dense sampling of bird diversity increases power of comparative genomics.

Authors:
Shaohong Feng Josefin Stiller Yuan Deng Joel Armstrong Qi Fang Andrew Hart Reeve Duo Xie Guangji Chen Chunxue Guo Brant C Faircloth Bent Petersen Zongji Wang Qi Zhou Mark Diekhans Wanjun Chen Sergio Andreu-Sánchez Ashot Margaryan Jason Travis Howard Carole Parent George Pacheco Mikkel-Holger S Sinding Lara Puetz Emily Cavill Ângela M Ribeiro Leopold Eckhart Jon Fjeldså Peter A Hosner Robb T Brumfield Les Christidis Mads F Bertelsen Thomas Sicheritz-Ponten Dieter Thomas Tietze Bruce C Robertson Gang Song Gerald Borgia Santiago Claramunt Irby J Lovette Saul J Cowen Peter Njoroge John Philip Dumbacher Oliver A Ryder Jérôme Fuchs Michael Bunce David W Burt Joel Cracraft Guanliang Meng Shannon J Hackett Peter G Ryan Knud Andreas Jønsson Ian G Jamieson Rute R da Fonseca Edward L Braun Peter Houde Siavash Mirarab Alexander Suh Bengt Hansson Suvi Ponnikas Hanna Sigeman Martin Stervander Paul B Frandsen Henriette van der Zwan Rencia van der Sluis Carina Visser Christopher N Balakrishnan Andrew G Clark John W Fitzpatrick Reed Bowman Nancy Chen Alison Cloutier Timothy B Sackton Scott V Edwards Dustin J Foote Subir B Shakya Frederick H Sheldon Alain Vignal André E R Soares Beth Shapiro Jacob González-Solís Joan Ferrer-Obiol Julio Rozas Marta Riutort Anna Tigano Vicki Friesen Love Dalén Araxi O Urrutia Tamás Székely Yang Liu Michael G Campana André Corvelo Robert C Fleischer Kim M Rutherford Neil J Gemmell Nicolas Dussex Henrik Mouritsen Nadine Thiele Kira Delmore Miriam Liedvogel Andre Franke Marc P Hoeppner Oliver Krone Adam M Fudickar Borja Milá Ellen D Ketterson Andrew Eric Fidler Guillermo Friis Ángela M Parody-Merino Phil F Battley Murray P Cox Nicholas Costa Barroso Lima Francisco Prosdocimi Thomas Lee Parchman Barney A Schlinger Bette A Loiselle John G Blake Haw Chuan Lim Lainy B Day Matthew J Fuxjager Maude W Baldwin Michael J Braun Morgan Wirthlin Rebecca B Dikow T Brandt Ryder Glauco Camenisch Lukas F Keller Jeffrey M DaCosta Mark E Hauber Matthew I M Louder Christopher C Witt Jimmy A McGuire Joann Mudge Libby C Megna Matthew D Carling Biao Wang Scott A Taylor Glaucia Del-Rio Alexandre Aleixo Ana Tereza Ribeiro Vasconcelos Claudio V Mello Jason T Weir David Haussler Qiye Li Huanming Yang Jian Wang Fumin Lei Carsten Rahbek M Thomas P Gilbert Gary R Graves Erich D Jarvis Benedict Paten Guojie Zhang

Nature 2020 11 11;587(7833):252-257. Epub 2020 Nov 11.

China National GeneBank, BGI-Shenzhen, Shenzhen, China.

Whole-genome sequencing projects are increasingly populating the tree of life and characterizing biodiversity. Sparse taxon sampling has previously been proposed to confound phylogenetic inference, and captures only a fraction of the genomic diversity. Here we report a substantial step towards the dense representation of avian phylogenetic and molecular diversity, by analysing 363 genomes from 92.4% of bird families-including 267 newly sequenced genomes produced for phase II of the Bird 10,000 Genomes (B10K) Project. We use this comparative genome dataset in combination with a pipeline that leverages a reference-free whole-genome alignment to identify orthologous regions in greater numbers than has previously been possible and to recognize genomic novelties in particular bird lineages. The densely sampled alignment provides a single-base-pair map of selection, has more than doubled the fraction of bases that are confidently predicted to be under conservation and reveals extensive patterns of weak selection in predominantly non-coding DNA. Our results demonstrate that increasing the diversity of genomes used in comparative studies can reveal more shared and lineage-specific variation, and improve the investigation of genomic characteristics. We anticipate that this genomic resource will offer new perspectives on evolutionary processes in cross-species comparative analyses and assist in efforts to conserve species.
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http://dx.doi.org/10.1038/s41586-020-2873-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759463PMC
November 2020

Acute Myeloid Leukemia with Gene Fusion Similar to Acute Promyelocytic Leukemia: Case Report and Literature Review.

Onco Targets Ther 2020 15;13:10559-10566. Epub 2020 Oct 15.

Department of Hematology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, People's Republic of China.

Retinoic acid receptor gamma (RARG) belongs to the nuclear receptor superfamily and has 90% homology to RAR alpha (RARA) and RAR beta. The () fusion gene has been implicated in acute promyelocytic leukemia (APL). gene rearrangement has been identified in a rare subtype of acute myeloid leukemia (AML) that resembles APL. To date, only 10 cases of gene rearrangements involving ( [], or []) have been reported. These patients show characteristics similar to APL, including bone marrow morphology, coagulation abnormality, and immunophenotype; however, they are resistant to all-trans retinoic acid and arsenic trioxide treatment. Moreover, there is no optimal therapeutic regimen for this subtype of AML. In this study, we report the clinical presentation and experimental findings of a case of AML with gene fusion similar to APL and review other cases of gene rearrangement described in the literature.
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http://dx.doi.org/10.2147/OTT.S273172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574910PMC
October 2020

Maternal Risk Factors for Lactation Mastitis: A Meta-analysis.

West J Nurs Res 2020 Oct 22:193945920967674. Epub 2020 Oct 22.

Department of Child Health Center, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

This meta-analysis was conducted to identify maternal risk factors for lactational mastitis. Studies published in English or Chinese were retrieved from Medline (PubMed), Embase, Cochrane Library, Web of Science, CNKI, WANFANG, and VIP databases according to predefined inclusion and exclusion criteria. Study quality was assessed by the Newcastle-Ottawa Scale. A random-effects model was used for data pooling and I tests to assess study heterogeneity. Pooled data from 8 cohorts and 10 case-control studies identified previous mastitis during breastfeeding (P<0.00001), cesarean section (P=0.001), breast trauma (P<0.001), anemia (P=0.0001), latch problems ≤ 8 weeks post-delivery (P=0.003), milk overproduction (P=0.002), blocked duct (P<0.0001), cracked nipple (especially ≤ 4 weeks post-delivery) (P=0.0001), use of nipple shields (P<0.00001), nipple cream (P<0.0001), brassieres (P<0.0001), and breast pumps (P<0.00001), and breastfeed duration > 30 min (P=0.008) as significant risk factors. Washing nipples before breastfeeding decreased lactational mastitis risk. Identification of these risk factors may facilitate the development of nursing care protocols for reducing lactational mastitis.
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http://dx.doi.org/10.1177/0193945920967674DOI Listing
October 2020

Can T-tube drainage be replaced by primary suture technique in laparoscopic common bile duct exploration? A meta-analysis of randomized controlled trials.

Langenbecks Arch Surg 2020 Dec 1;405(8):1209-1217. Epub 2020 Oct 1.

Gansu Provincial Hospital, Lanzhou, China.

Background: Although laparoscopic common bile duct exploration (LCBDE) is considered the best treatment and has the advantages of being minimally invasive for common bile duct (CBD) stones, the choice of T-tube drainage (TTD) or primary duct closure (PDC) after LCBDE is still controversial. Therefore, the aim of the study was to compare the superiority of PDC versus TTD after LCBDE for choledocholithiasis.

Methods: All potential studies which compare the surgical effects between PDC with TTD were electronically searched for in PubMed, Web of Science, and the Cochrane library databases up to November 2019. Data synthesis and statistical analysis were carried out using RevMan 5.3 software.

Results: In total, six randomized controlled trials with 604 patients (307 in the PDC group and 297 in the TTD group) were included in the current meta-analysis. As compared with the TTD group, the pooled data showed that PDC group had shorter operating time (WMD = -24.30; 95% CI = -27.02 to -21.59; p < 0.00001; I = 0%; p < 0.88), less medical expenditure (WMD = -2255.73; 95% CI = -3330.59 to -1180.86; p < 0.0001; I = 96%; p < 0.00001), shorter postoperative hospital stay (OR = -2.88; 95% CI = -3.22 to -2.54; p < 0.00001; I = 60%; p < 0.03), and lower postoperative complications (OR = 0.49; 95% CI = 0.31 to 0.78; p = 0.77; I = 0%; p = 0.003). There were no significant differences between the two groups concerning bile leakage (OR = 0.74; 95% CI = 0.36 to 1.53; p = 0.42; I = 0%; p = 0.90) and retained stones (OR = 0.96; 95% CI = 0.36 to 2.52; p < 0.93; I = 0%; p < 0.66).

Conclusions: LCBDE with PDC should be performed as a priority alternative compared with TTD for choledocholithiasis.
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http://dx.doi.org/10.1007/s00423-020-02000-zDOI Listing
December 2020

ASPM Predicts Poor Clinical Outcome and Promotes Tumorigenesis for Diffuse Large B-cell Lymphoma.

Curr Cancer Drug Targets 2021 ;21(1):80-89

Department of Oncology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, China.

Background: Abnormal spindle-like microcephaly-associated protein (ASPM) has been implicated in the aggressive behavior of several malignant tumors. However, its potential effects on diffuse large B-cell lymphoma (DLBCL) still remain unknown.

Methods: ASPM levels were determined by immunohistochemically in DLBCL tissues from 54 patients and 15 reactive lymphoid hyperplasia (RLH) tissues as control, and its association with clinical features and overall survival were evaluated. The effects of ASPM on cell growth, cell apoptosis and cell cycle of DLBCL cells were assessed. Bioinformatics, quantitative RT-PCR and western blotting were conducted for mechanic investigation.

Results: ASPM expression was upregulated in DLBCL tissues compared with RLH tissues. Its high expression was correlated with inferior clinicopathological characteristics and poor outcomes of DLBCL patients. Multivariate analysis revealed that high ASPM expression emerged as an independent factor for poor prognosis. In DLBCL cell lines, silencing of ASPM suppressed cell growth, induced cell apoptosis and arrested the cell cycle. Mechanically, effects of ASPM knockdown on DLBCL cells were partially dependent on its block of the Wnt/β-catenin pathway.

Conclusion: Collectively, our results suggested that ASPM potentially served as a predictive biomarker of DLCBL tumorigenesis and prognosis, representing a potential therapeutic target for DLCBL.
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http://dx.doi.org/10.2174/1568009620666200915090703DOI Listing
January 2021

Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid-related diseases.

FEBS J 2020 Aug 15. Epub 2020 Aug 15.

Hunan Institute of Animal & Veterinary Science, Changsha, China.

Disorder of lipid metabolism has become an urgent health problem that brings about a variety of metabolic syndromes, including hepatic steatosis, adipose tissue dysfunction, diabetes and obesity. Circular RNAs (circRNAs), a class of emerging RNA molecules with unique structure and extensive effects, have been verified to participate in various biological programs through distinct mechanisms, especially in lipid-related processes. In this review, the biogenesis, characteristics, and functional mechanisms of circRNAs are discussed. Furthermore, the methods for circRNA identification and expression profiles of circRNAs associated with adipogenesis and lipid metabolism are described. Additionally, we emphasize the regulatory roles of circRNAs in adipogenesis, lipid metabolism, and lipid-related diseases. Finally, the diagnostic and therapeutic potential of circRNAs is highlighted, showing potential for the clinical application of circRNAs in the treatment of lipid-related diseases in the near future.
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http://dx.doi.org/10.1111/febs.15525DOI Listing
August 2020

Decreased Relative Cerebral Blood Flow in Unmedicated Heroin-Dependent Individuals.

Front Psychiatry 2020 14;11:643. Epub 2020 Jul 14.

Department of Radiology, Second Xiangya Hospital of Central South University, Changsha, China.

Understanding the brain mechanisms of heroin dependence is invaluable for developing effective treatment. Measurement of regional cerebral blood flow (CBF) provides a method to visualize brain circuits that are functionally impaired by heroin dependence. This study examined regional CBF alterations and their clinical associations in unmedicated heroin-dependent individuals (HDIs) using a relatively large sample. Sixty-eight (42 males, 26 females; age: 40.9 ± 7.3 years) HDIs and forty-seven (34 males, 13 females; age: 39.3 ± 9.2 years) matched healthy controls (HCs) underwent high-resolution T1 and whole-brain arterial spin labeling (ASL) perfusion magnetic resonance imaging (MRI) scans. Additionally, clinical characteristics were collected for neurocognitive assessments. HDIs showed worse neuropsychological performance than HCs and had decreased relative CBF (rCBF) in the bilateral middle frontal gyrus (MFG), inferior temporal gyrus, precuneus, posterior cerebellar lobe, cerebellar vermis, and the midbrain adjacent to the ventral tegmental area; right posterior cingulate gyrus, thalamus, and calcarine. rCBF in the bilateral MFG was negatively correlated with Trail Making Test time in HDIs. HDIs had limbic, frontal, and parietal hypoperfusion areas. Low CBF in the MFG indicated cognitive impairment in HDIs. Together, these findings suggest the MFG as a critical region in HDIs and suggest ASL-derived CBF as a potential marker for use in heroin addiction studies.
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http://dx.doi.org/10.3389/fpsyt.2020.00643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372972PMC
July 2020

Demethylase-independent function of JMJD2D as a novel antagonist of p53 to promote Liver Cancer initiation and progression.

Theranostics 2020 11;10(19):8863-8879. Epub 2020 Jul 11.

Department of Hepatobiliary Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, China.

As a histone demethylase, JMJD2D can enhance gene expression by specifically demethylating H3K9me2/3 and plays an important role in promoting colorectal cancer progression. However, its role in liver cancer remains unclear. The expression of JMJD2D was examined in human liver cancer specimens and non-tumorous liver tissues by immunohistochemical or immunoblot analysis. JMJD2D expression was knocked down in liver cancer cells using small hairpin RNAs, and cells were analyzed with Western blot, real-time PCR, cell viability, colony formation, and flow cytometry assays. Cells were also grown as tumor xenografts in nude mice, and the tumor cell proliferation and apoptosis were measured by immunohistochemical analysis. The relationship between JMJD2D and p53 was studied by co-immunoprecipitation, chromatin immunoprecipitation, and electric mobility shift assay. Wild-type and JMJD2D-knockout mice were intraperitoneally injected with diethylnitrosamine (DEN) to induce liver tumors and the liver cancer initiation and progression were investigated. JMJD2D was frequently upregulated in human liver cancer specimens compared with non-tumorous liver tissues. The overall survival of liver cancer patients with high JMJD2D expression was significantly decreased compared to that with low JMJD2D expression. JMJD2D knockdown reduced liver cancer cell proliferation and xenograft tumor growth, sensitized cells to chemotherapeutic drug-induced apoptosis, and increased the expression of cell cycle inhibitor p21 and pro-apoptosis gene PUMA. Genetically, JMJD2D deficiency protected mice against DEN-induced liver cancer initiation and progression. Knockout of tumor suppressor p53 significantly reduced the effects of JMJD2D knockdown on cell proliferation, apoptosis, and the expression of p21 and PUMA, suggesting that JMJD2D regulates liver cancer cell functions in part through inhibiting p53 signaling pathway. Mechanistically, JMJD2D directly interacted with p53 and inhibited p53 recruitment to the p21 and PUMA promoters in a demethylation activity-independent manner, implicating a demethylase-independent function of JMJD2D as a novel p53 antagonist. In addition, JMJD2D could activate Wnt/β-catenin signaling to promote liver cancer cell proliferation. Our study demonstrates that JMJD2D can antagonize the tumor suppressor p53 and activate an oncogenic signaling pathway (such as Wnt/β-catenin signaling pathway) simultaneously to promote liver cancer initiation and progression, suggesting that JMJD2D may serve as a novel target for liver cancer treatment.
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http://dx.doi.org/10.7150/thno.45581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392006PMC
July 2020

Increased Amplitude of Low-Frequency Fluctuation in Right Angular Gyrus and Left Superior Occipital Gyrus Negatively Correlated With Heroin Use.

Front Psychiatry 2020 3;11:492. Epub 2020 Jul 3.

Department of Radiology, The Second Xiangya Hospital, Central South University, Changsha, China.

Abnormal amplitude of low-frequency fluctuation has been implicated in heroin addiction. However, previous studies lacked consistency and didn't consider the impact of confounding factors such as methadone and alcohol. Fifty-one heroin-dependent (HD) individuals and 40 healthy controls underwent resting-state functional magnetic resonance imaging. The 'amplitude of low-frequency fluctuation' (ALFF) value was calculated and support vector machine (SVM) classification analysis was applied to analyze the data. Compared with healthy controls, heroin addicts exhibited increased ALFF in the right angular gyrus (AG) and left superior occipital gyrus (SOG). A negative correlation was observed between increased ALFF in the right angular gyrus and left superior occipital gyrus and the duration of heroin use (=0.004, =-0.426; =0.009, =-0.361). Moreover, the ALFF in the right AG and left SOG could discriminate the HD subjects from the controls with acceptable accuracy (Acc=64.85%, =0.004; Acc=63.80%, =0.005). HD patients showed abnormal ALFF in the brain areas involved in semantic memory and visual networks. The longer HD individuals abused heroin, the less the ALFF of associated brain regions increased. These observed patterns suggested that the accumulative effect of heroin's neurotoxicity overpowered self-recovery of the brain and may be applied as a potential biomarker to identify HD individuals from the controls.
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http://dx.doi.org/10.3389/fpsyt.2020.00492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350776PMC
July 2020

Distinct antibacterial activity of a vertically aligned graphene coating against Gram-positive and Gram-negative bacteria.

J Mater Chem B 2020 07;8(28):6069-6079

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 200011, P. R. China.

Graphene-based nanomaterials (GBNs) are known to exhibit biocidal activities, however, the combined effect of GBNs based on physical disruption and oxidative stress on different types of bacteria remain unclear. Here, we use both Gram-negative (Escherichia coli and Salmonella typhimurium) and Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) bacteria to investigate the antimicrobial properties of vertically and horizontally aligned graphenes grown on semiconductor silicon (Si) and insulator silicon dioxide (SiO2). It is found that the bacteria show different sensitivity to isomeric-structured GBNs. Gram-negative bacteria are more vulnerable to graphene-coated Si substrates than to SiO2, because the less negatively charged membrane enhances the electron transfer effect that extracts the electrons from the microbial membranes, and Gram-positive bacteria seem to show more susceptibility to physical puncturing of vertically aligned graphene than to horizontally aligned graphene due to the nature of the compound and the shape of the membrane structure. Subsequently, the vertically aligned graphene Si substrate (G@V-Si) exerts the superior antimicrobial ability on all the bacteria. Finally, all the above GBNs show low cytotoxicity and high biocompatibility, and the robust in vivo antibacterial effect indicates that G@V-Si could serve as an ideal platform for antimicrobial treatment.
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http://dx.doi.org/10.1039/d0tb00417kDOI Listing
July 2020

DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1.

Onco Targets Ther 2020 15;13:4201-4211. Epub 2020 May 15.

Department of Otorhinolaryngology, Head and Neck Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, People's Republic of China.

Introduction: Head and neck squamous cell carcinoma (HNSCC), which rank the 7th malignant tumors worldwide, is closely related to methylation and HPV infection. Ionizing radiation therapy is the main strategy for HNSCC patients in advanced stage. Previously, HPV-positive HNSCC predict better prognosis than HPV-negative HNSCCs under radiotherapy, however its molecular mechanism is unresolved. SMG1 serves as a potential tumor suppressor in various cancers, including HNSCC.

Methods: The mRNAs and proteins expression of HPV E6/E7, p16, p53, DNMT1, SMG1 were detected after different treatments by qPCR and Western blot. The clone formation ability was measured in radiation dose after different treatments.

Results: In our study, the expression of HPV16 E6, DNA Methyltransferase 1(DNMT1) and SMG1 in head and neck carcinomas cell lines was detected by RT-qPCR and Western blot. Forced E6 level in HPV-negative cells by overexpression plasmid promoted the expression of DNMT1, which resulted in decreased SMG1 expression. Silenced SMG1 in HPV-negative HNSCC cells elicited increased radiation sensitivity, suggesting that SMG1 may be an effective switch to regulate the effect of radiotherapy in HNSCC.

Conclusion: Our study indicated that DNMT1 enhances the radiosensitivity of HPV-positive head and neck squamous cell carcinomas via downregulating SMG1.
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http://dx.doi.org/10.2147/OTT.S227395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237113PMC
May 2020

Endoscopic Ossiculoplasty for the Management of Isolated Congenital Ossicular Chain Malformation: Surgical Results in 16 Ears.

Ear Nose Throat J 2020 Jun 4:145561320931218. Epub 2020 Jun 4.

Department of Otorhinolaryngology, Head and Neck Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Objective: The aim of this study is to describe the clinical characteristics and intraoperative findings and further evaluate the efficacy of endoscopic ossiculoplasty for the management of isolated congenital ossicular chain malformation.

Methods: A retrospective study was performed on 16 ears (15 patients) with the isolated congenital ossicular chain malformation who underwent endoscopic ossiculoplasty in our department from May 2017 to January 2019. Endoscopic exploratory tympanotomy was conducted to check the ossicular chain; at the same time, endoscopic ossiculoplasty was performed depending on intraoperative findings. Air-conduction thresholds, bone-conduction thresholds, and air-bone gaps (ABGs) were measured before and after surgery, and the hearing outcome was assessed at 6 months postoperatively.

Results: The most common malformations of ossicular chain were the missing of the incus long process and stapes suprastructure. A serial assessment of the hearing status was conducted before and 6 months after surgery. It showed the mean postoperative pure-tone average (PTA) was significantly reduced, and the mean postoperative ABG was obviously closed, respectively ( < .001). The mean PTA gain was 36.3 ± 8.6 dB, and the ABG closure was 35.1 ± 8.3 dB; ABG closure to 20 dB or less and ABG closure to 10 dB or less were achieved in 14 cases (87.5%) and 5 cases (31.3%), respectively. No differences were observed in postoperative hearing outcome between type Ⅲ cases and type Ⅳ cases; however, cases with partial ossicular replacement prosthesis implantation showed a larger hearing gain ( = .049) and a higher proportion of postoperative ABG less than 10 dB ( = .021). No facial palsy and significant sensorineural hearing loss occurred; all patients completed the surgery without the need of canalplasty, and the chorda tympani nerve was preserved in all patients.

Conclusions: This research showed endoscopic surgery was effective in the diagnosis and management of isolated congenital ossicular chain malformation; the endoscopic ossiculoplasty provides an alternative method to manage congenital ossicular chain malformation, with comfortable hearing outcome and the advantage of excellent vision and less invasion.
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http://dx.doi.org/10.1177/0145561320931218DOI Listing
June 2020

Orbital malformation with dysplastic infraorbital artery in neurofibromatosis type 1.

Eur J Ophthalmol 2020 May 6:1120672120922447. Epub 2020 May 6.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: To report a case with neurofibromatosis type 1 presenting as prominent enophthalmos and abnormal infraorbital artery.

Case Description: A 19-year-old man with a family history of neurofibromatosis presented with prominent right enophthalmos. Computed tomography showed orbital dysplasia and enlarged inferior orbital fissure but no plexiform neurofibroma. Prominent intraoperative hemorrhage originated from several abnormal arteries in the infraorbital region during orbital reconstruction. A tortuous and dysplastic infraorbital artery was verified postoperatively by computed tomography angiography. The bleeding vessels were supposed to be the orbital branches of the dysplastic infraorbital artery.

Conclusions: The orbital malformation and enlargement of inferior orbital fissure probably resulted in an abnormal infraorbital artery. Selective artery embolization may be chosen as a preceding treatment before orbital reconstruction surgery.
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http://dx.doi.org/10.1177/1120672120922447DOI Listing
May 2020

Delayed Orbital Apex Syndrome in the Post-Reconstructed Orbit.

J Craniofac Surg 2020 Jul-Aug;31(5):e481-e483

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine.

Delayed orbital apex syndrome (OAS) is rare during orbital blowout fracture reconstruction. A 30-year-old woman fractured the right orbital floor in a fall and undergone repairing operation 2 weeks later. After severe sneezing on postoperative day 10, she gradually arose vision loss, ophthalmoplegia, ptosis with a dilated and fixed pupil within few hours, then consulted our department and was diagnosed as OAS. Computed tomography scan showed displaced implant and retrobulbar emphysema resulting in a constellation of compression to orbital apex. Therefore, an exploratory operation was engaged to reposition the implant and reduce the emphysema concurrent with mega-dose steroids. The patient regained vision immediately and resolved all symptoms at the 6 months follow-up.
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http://dx.doi.org/10.1097/SCS.0000000000006490DOI Listing
November 2020

Semiconductor glass with superior flexibility and high room temperature thermoelectric performance.

Sci Adv 2020 Apr 10;6(15):eaaz8423. Epub 2020 Apr 10.

Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Most crystalline inorganic materials, except for metals and some layer materials, exhibit bad flexibility because of strong ionic or covalent bonds, while amorphous materials usually display poor electrical properties due to structural disorders. Here, we report the simultaneous realization of extraordinary room temperature flexibility and thermoelectric performance in AgTe S -based materials through amorphization. The coexistence of amorphous main phase and crystallites results in exceptional flexibility and ultralow lattice thermal conductivity. Furthermore, the flexible AgTeS glass exhibits a degenerate semiconductor behavior with a room temperature Hall mobility of ~750 cm V s at a carrier concentration of 8.6 × 10 cm, which is at least an order of magnitude higher than other amorphous materials, leading to a thermoelectric power factor also an order of magnitude higher than the best amorphous thermoelectric materials known. The in-plane prototype uni-leg thermoelectric generator made from this material demonstrates its potential for flexible thermoelectric device.
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http://dx.doi.org/10.1126/sciadv.aaz8423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148084PMC
April 2020

Approaching high-performance of ordered structure SbTe film via unique angular intraplanar grain boundaries.

Sci Rep 2020 Apr 6;10(1):5978. Epub 2020 Apr 6.

Beijing Key Laboratory of Special Functional Materials and Films, School of Materials Science and Engineering, Beihang University, Beijing, 100191, China.

In this paper, we present an innovative electric-field-assisted magnetron-sputtering deposition method for films preparation. By grain boundary-engineering, we successeful obtained the ordered SbTe film with greatly high figure of merit via controlling external electric field. It has been found that the electric field can induce the change in the angle of intraplanar grain boundaries between (0 1 5) and (0 1 5) planes, which leads to the enhanced holes mobility and maintained low thermal conductivity. The energy filtering takes place at the angular intraplanar grain boundaries. At room temperature, a high ZT value of 1.75 can be achieved in the deposited SbTe film under 30 V external electric field. This is a very promising approach that the electric field induced deposition can develop high-performance SbTe-based thermoelectric films.
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http://dx.doi.org/10.1038/s41598-020-63062-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136274PMC
April 2020

Enhanced Electrical Transport Properties via Defect Control for Screen-Printed BiTe Films over a Wide Temperature Range.

ACS Appl Mater Interfaces 2020 Apr 27;12(14):16630-16638. Epub 2020 Mar 27.

School of Materials Science and Engineering, Beihang University, Beijing 100083, China.

The application of screen-printed thin-film thermoelectric (TE) devices is still in its infancy, mainly due to low TE performance of screen-printed films and especially the poor electrical transport properties. Herein, we design and prepare a high-performance screen-printed BiTe film through introducing excessive Te-based nanosolder (Te-NS) to simultaneously realize the conduction channel construction and defect control. On one hand, the promoted carrier migration makes the electrical conductivity dramatically rise about 7 times, with a maximum power factor of 4.65 μW cm K . Meanwhile, the defect formation mechanism in the screen-printed BiTe film after the introduction of Te-NS is also in-depth studied, and the bipolar conduction is reduced by increased generation of and/or more suppression of , resulting in a postponed temperature of the maximum Seebeck coefficient. Hence, the large engineering power factor is achieved with excellent temperature linearity, indicating a possibility of screen-printed film application in a large temperature region. A TE device with a single leg has been fabricated to further demonstrate the generation validity. An open-circuit voltage of 11.34 mV and a maximum output power of 27.1 μW at a temperature gradient of 105 K have been achieved over a wide temperature range from 303 to 478 K. This study provides a theoretical and practical basis for the performance improvement of screen-printed TE films and devices.
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http://dx.doi.org/10.1021/acsami.0c01049DOI Listing
April 2020

Haplotype diversity and phylogenetic characteristics for Guanzhong Han population from Northwest China via 38 Y-STRs using Yfiler™ Platinum Amplification System.

Mol Genet Genomic Med 2020 05 12;8(5):e1187. Epub 2020 Mar 12.

Center of Forensic Expertise, Affiliated hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Background: For better application in human forensic cases and population genetics research, it is imperative to investigate the genetic characteristics of Guanzhong Han population using enhanced Y-chromosomal short tandem repeats (Y-STR) detecting system with higher discriminating power than previous ones.

Methods: In this study, 38 Y-STRs were profiled in 430 unrelated Chinese Han male individuals from Guanzhong region of Shaanxi Province, Northwest China, using the Yfiler™ Platinum PCR Amplification Kit. Haplotype frequencies and forensic parameters were calculated. Comprehensive population comparisons with geographically/ethnically different populations in China and other worldwide countries were performed.

Results: A total of 422 different haplotypes were observed with the overall haplotype diversity (HD), discriminatory power (DC) and haplotype match probability (HMP) were 0.9999, 0.9814, and 0.0024, respectively. Guanzhong Han showed genetically affinity with Han ethnicity from Shanxi and Henan provinces, while far distant from Tibetan populations.

Conclusion: This study offered a unique insight into Guanzhong Han population, the 38 Y-STRs included in the the Yfiler™ Platinum system are highly polymorphic and informative and can be used for forensic practice and human genetic research.
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http://dx.doi.org/10.1002/mgg3.1187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216798PMC
May 2020

Activation of GPR43 suppresses TNF-α-induced inflammatory response in human fibroblast-like synoviocytes.

Arch Biochem Biophys 2020 05 5;684:108297. Epub 2020 Feb 5.

Department of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China. Electronic address:

Although rheumatoid arthritis (RA) has long posed a major threat to global health, the mechanisms driving the development and progression of RA remain incompletely understood. In the present study, we investigated the effects of G protein-coupled receptor 43 (GPR43/FFAR2) in various aspects of the pathogenesis of RA. To our knowledge, this is the first study to demonstrate that GPR43 is expressed on human fibroblast-like synoviocytes (FLS). Furthermore, we show that GPR43 is upregulated in FLS exposed to tumor necrosis factor-α (TNF-α). Importantly, our findings demonstrate that activation of GPR43 using its specific agonist significantly suppressed expression of the following key factors of RA: cytokines, such as interleukin-6 (IL-6), IL-8, high mobility group protein 1 (HMG-1); chemokines, such as monocyte chemoattractant protein 1 (MCP-1), intercellular adhesion molecule 1 (ICAM-1), and vascular cellular adhesion molecule 1 (VCAM-1); markers of oxidative stress, such as production of reactive oxygen species (ROS) and 4-hydroxynoneal (4-HNE); degradative enzymes, such as matrix metalloproteinase-3 (MMP-3) and MMP-13; and activation of the nuclear factor-κB (NF-κB) inflammatory signaling pathway. These results suggest a promising potential role for GPR43 as a specific target in the treatment and prevention of RA.
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http://dx.doi.org/10.1016/j.abb.2020.108297DOI Listing
May 2020