Publications by authors named "Yu-jun Wang"

113 Publications

Novel selective κ agonists SLL-039 and SLL-1206 produce potent antinociception with fewer sedation and aversion.

Acta Pharmacol Sin 2021 Sep 7. Epub 2021 Sep 7.

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

SLL-039 (N-cyclopropylmethyl-7α-4'-(N'-benzoyl) amino-phenyl-6,14-endoethano-tetrahydronorthebaine) and SLL-1206 (N-cyclopropylmethyl-7α-3'-(p-methoxybenzyl) amino-phenyl-6,14-endoethano-tetrahydronorthebaine) are two 4,5-epoxymorphinan-based high selective κ receptor agonists that we recently discovered. In the present study we characterized their pharmacological properties in comparison with arylacetamide-based typical κ agonist U50,488H. We showed that both SLL-039 and SLL-1206 produced potent and long-lasting antinociceptive actions in three different rodent models of pain via activation of κ opioid receptor. In hot-plate assay, the antinociceptive potency of SLL-039 and SLL-1206 increased about 11-and 17.3-fold compared to U50,488H and morphine, respectively, with ED values of 0.4 mg/kg. Following repeated administration, SLL-1206, SLL-039, and U50,488H all developed analgesic tolerance tested in hot-plate assay. U50,488H and SLL-039 produced antipruritic effects in a dose-dependent manner, whereas SLL-1206 displayed some antipruritic effects only at very low doses. In addition, SLL-1206 was capable of decreasing morphine-induced physical dependence. More importantly, SLL-039 and SLL-1206 at effective analgesic doses did not cause sedation and conditioned place aversion (CPA), whereas U50,488H did. In comparison with SLL-039, SLL-1206 caused similar antinociceptive responses, but fewer sedation and CPA. In conclusion, our results suggest that SLL-039 and SLL-1206 have potential to be developed as novel analgesic agents, and 4,5-expoxymorphinan scaffold is an attractive structure for the development of selective κ agonists with fewer side effects.
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http://dx.doi.org/10.1038/s41401-021-00761-xDOI Listing
September 2021

Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development.

Zygote 2021 Jun 23:1-9. Epub 2021 Jun 23.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining810001, China.

Environmental hypoxia exposure causes fertility problems in human and animals. Compelling evidence suggests that chronic hypoxia impairs spermatogenesis and reduces sperm motility. However, it is unclear whether paternal hypoxic exposure affects fertilization and early embryo development. In the present study, we exposed male mice to high altitude (3200 m above sea level) for 7 or 60 days to evaluate the effects of hypoxia on sperm quality, zygotic DNA methylation and blastocyst formation. Compared with age-matched controls, hypoxia-treated males exhibited reduced fertility after mating with normoxic females as a result of defects in sperm motility and function. Results of in vitro fertilization (IVF) experiments revealed that 60 days' exposure significantly reduced cleavage and blastocyst rates by 30% and 70%, respectively. Immunohistochemical staining of pronuclear formation indicated that the pronuclear formation process was disturbed and expression of imprinted genes was reduced in early embryos after paternal hypoxia. Overall, the findings of this study suggested that exposing male mice to hypoxia impaired sperm function and affected key events during early embryo development in mammals.
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http://dx.doi.org/10.1017/S0967199421000216DOI Listing
June 2021

Amygdala dynorphin/κ opioid receptor system modulates depressive-like behavior in mice following chronic social defeat stress.

Acta Pharmacol Sin 2021 May 25. Epub 2021 May 25.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Major depression disorder is a severe and recurrent neuropsychological disorder characterized by lowered mood and social activity and cognitive impairment. Owing to unclear molecular mechanisms of depression, limited interventions are available in clinic. In this study we investigated the role of dynorphin/κ opioid receptor system in the development of depression. Mice were subjected to chronic social defeat stress for 14 days. Chronic social defeat stress induced significant social avoidance in mice characterized by decreased time duration in the interaction zone and increased time duration in the corner zone. Pre-administration of a κ opioid receptor antagonist norBNI (10 mg/kg, i.p.) could prevent the development of social avoidance induced by chronic social defeat stress. Social avoidance was not observed in κ opioid receptor knockout mice subjected to chronic social defeat stress. We further revealed that social defeat stress activated c-fos and ERK signaling in the amygdala without affecting the NAc, hippocampus and hypothalamus, and ERK activation was blocked by systemic injection of norBNI. Finally, the expression of dynorphin A, the endogenous ligand of κ opioid receptor, was significantly increased in the amygdala following social defeat stress; microinjection of norBNI into the amygdala prevented the development of depressive-like behaviors caused by social defeat stress. The present study demonstrates that upregulated dynorphin/κ opioid receptor system in the amygdala leads to the emergence of depression following chronic social defeat stress, and sheds light on κ opioid receptor antagonists as potential therapeutic agents for the prevention and treatment of depression following chronic stress.
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http://dx.doi.org/10.1038/s41401-021-00677-6DOI Listing
May 2021

Alteration of twinfilin1 expression underlies opioid withdrawal-induced remodeling of actin cytoskeleton at synapses and formation of aversive memory.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Exposure to drugs of abuse induces alterations of dendritic spine morphology and density that has been proposed to be a cellular basis of long-lasting addictive memory and heavily depend on remodeling of its underlying actin cytoskeleton by the actin cytoskeleton regulators. However, the actin cytoskeleton regulators involved and the specific mechanisms whereby drugs of abuse alter their expression or function are largely unknown. Twinfilin (Twf1) is a highly conserved actin-depolymerizing factor that regulates actin dynamics in organisms from yeast to mammals. Despite abundant expression of Twf1 in mammalian brain, little is known about its importance for brain functions such as experience-dependent synaptic and behavioral plasticity. Here we show that conditioned morphine withdrawal (CMW)-induced synaptic structure and behavior plasticity depends on downregulation of Twf1 in the amygdala of rats. Genetically manipulating Twf1 expression in the amygdala bidirectionally regulates CMW-induced changes in actin polymerization, spine density and behavior. We further demonstrate that downregulation of Twf1 is due to upregulation of miR101a expression via a previously unrecognized mechanism involving CMW-induced increases in miR101a nuclear processing via phosphorylation of MeCP at Ser421. Our findings establish the importance of Twf1 in regulating opioid-induced synaptic and behavioral plasticity and demonstrate its value as a potential therapeutic target for the treatment of opioid addiction.
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http://dx.doi.org/10.1038/s41380-021-01111-3DOI Listing
May 2021

Mitochondrial genome of (Trichoptera).

Mitochondrial DNA B Resour 2021 Mar 31;6(4):1315-1316. Epub 2021 Mar 31.

Ocean College, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, PR China.

Trichoptera are a group of the benthic organism, almost all of which live in water during their life cycle. Trichoptera usually develop through egg, larva, pupa, and moth stages. In its larval stage, Trichoptera usually live in water and are often called the caddisfly. In this study, the mitochondrial genome of was analyzed. The total length of the mitochondrial genome is 15,424 bp and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The genome has a typical mitochondrial gene sequence of Trichoptera. Phylogenetic analysis of the mitochondrial genomes of 23 species of Trichoptera and Lepidoptera showed that forms a monophyletic group with other species of Lepidoptera.
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http://dx.doi.org/10.1080/23802359.2021.1907805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018442PMC
March 2021

[Influence of Burning Fireworks on the Atmosphere During the Spring Festival in Guangzhou in 2020].

Huan Jing Ke Xue 2021 Feb;42(2):546-555

Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China.

Twenty-one air quality monitoring stations including four with single particle aerosol mass spectrometers (SPAMS) were used to observe air quality and aerosol particulates during the 2020 Spring Festival (from January 21 to 28) in Guangzhou. The effect of burning fireworks on the atmosphere of Guangzhou and its eleven administrative regions was examined, and the chemical composition of firework particles was detected and analyzed by single particle aerosol mass spectrometry. The results show that the burning of fireworks had a significant impact on air quality in the discharge area and the prohibited discharge area. The concentrations of PM, PM, and SO sharply increased in Guangzhou on New Year's Eve. Air quality in Zengcheng District, Baiyun District, Huangpu District, and some areas of Tianhe District was also affected by the concentrated burning of fireworks on January 25 between 01:00 and 06:00. A method of fireworks tracing based on SPAMS using Al as a tracer was established with a time resolution of 5 min. The main particle types emitted by the burning fireworks were levoglucan, potassium-rich, and mineral. These particles were well mixed with nitrate, but this was not conducive to the formation of ammonium.
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http://dx.doi.org/10.13227/j.hjkx.202006045DOI Listing
February 2021

Characterization of the complete mitochondrial genome of (Trichoptera, Stenopsychidae).

Mitochondrial DNA B Resour 2020 Aug 3;5(3):3114-3115. Epub 2020 Aug 3.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, PR China.

Caddisflies of (Trichoptera, Stenopsychidae) are widely distributed in various freshwater bodies and a few species inhabit marine environments. The mitochondrial genome was sequenced by Illumina high-throughput sequencing, and then the complete mitochondrial genome sequence was obtained through splicing and assembly. The mitochondrial genome sequence size was 15,371 bp, comprising 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Two of the protein-coding genes ( and ) had an incomplete termination codon T. In addition, the start codon of all protein-coding genes was ATN, except for the start codon of the gene which was GTG. The base composition of the mitochondrial genome was 41.64% A, 35.03% T, 7.81% G, and 15.52% C.
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http://dx.doi.org/10.1080/23802359.2020.1797564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782142PMC
August 2020

Genome-Wide Identification of the Expansin Gene Family and Its Potential Association with Drought Stress in Moso Bamboo.

Int J Mol Sci 2020 Dec 14;21(24). Epub 2020 Dec 14.

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.

Expansins, a group of cell wall-loosening proteins, are involved in cell-wall loosening and cell enlargement in a pH-dependent manner. According to previous study, they were involved in plant growth and abiotic stress responses. However, information on the biological function of the expansin gene in moso bamboo is still limited. In this study, we identified a total of 82 expansin genes in moso bamboo, clustered into four subfamilies (α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA) and expansin-like B (EXPB)). Subsequently, the molecular structure, chromosomal location and phylogenetic relationship of the expansin genes of ( were further characterized. A total of 14 pairs of tandem duplication genes and 31 pairs of segmented duplication genes were also identified, which may promote the expansion of the expansin gene family. Promoter analysis found many cis-acting elements related to growth and development and stress response, especially abscisic acid response element (ABRE). Expression pattern revealed that most have tissue expression specificity. Meanwhile, the expression of some selected was significantly upregulated mostly under abscisic acid (ABA) and polyethylene glycol (PEG) treatment, which implied that these genes actively respond to expression under abiotic stress. This study provided new insights into the structure, evolution and function prediction of the expansin gene family in moso bamboo.
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http://dx.doi.org/10.3390/ijms21249491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764852PMC
December 2020

High-Flow Nasal Cannula for Chronic Obstructive Pulmonary Disease with Acute Compensated Hypercapnic Respiratory Failure: A Randomized, Controlled Trial.

Int J Chron Obstruct Pulmon Dis 2020 24;15:3051-3061. Epub 2020 Nov 24.

Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Introduction: Currently, there is a lack of evidence on the utilization of high-flow nasal cannula (HFNC) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) accompanied by hypercapnic respiratory failure. We aimed to explore the efficacy and safety of HFNC compared with conventional oxygen therapy (COT) in such patients.

Methods: This was a prospective, randomized, controlled trial. Patients with AECOPD with a baseline arterial blood gas pH ≥7.35, PaO <60 mmHg, and PaCO >45 mmHg were enrolled. The primary endpoint was treatment failure, which needs mechanical ventilation.

Results: A total of 320 patients were randomized to either the HFNC group (n = 160) or the COT group (n = 160). Sixteen (10.0%) patients in the HFNC group had treatment failure during hospitalization, which was significantly lower than the COT group figure of 31 (19.4%) patients ( = 0.026). Twenty-four hours after recruitment, the PaCO of the HFNC group was lower than that of the COT group (54.1 ± 9.79 mmHg vs 56.9 ± 10.1 mmHg, = 0.030). PaCO higher than 59 mmHg after HFNC for 24 h was identified as an independent risk factor for treatment failure [OR 1.078, 95% CI 1.006-1.154, = 0.032].

Conclusion: In AECOPD patients with acute compensated hypercapnic respiratory failure, HFNC improved the prognosis compared with COT. Therefore, HFNC might be considered for first-line oxygen therapy in select patients.

Trial Registration Number: ClinicalTrials.Gov: NCT02439333.
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http://dx.doi.org/10.2147/COPD.S283020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699989PMC
June 2021

Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies.

PLoS One 2020 25;15(8):e0237744. Epub 2020 Aug 25.

Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

Both the Mediterranean (MED) species of the Bemisia tabaci whitefly complex and the greenhouse whitefly (Trialeurodes vaporariorum, TV) are important agricultural pests. The two species of whiteflies differ in many aspects such as morphology, geographical distribution, host plant range, plant virus transmission, and resistance to insecticides. However, the molecular basis underlying their differences remains largely unknown. In this study, we analyzed the genetic divergences between the transcriptomes of MED and TV. In total, 2,944 pairs of orthologous genes were identified. The average identity of amino acid sequences between the two species is 93.6%. The average nonsynonymous (Ka) and synonymous (Ks) substitution rates and the ratio of Ka/Ks of the orthologous genes are 0.0389, 2.23 and 0.0204, respectively. The low average Ka/Ks ratio indicates that orthologous genes tend to be under strong purified selection. The most divergent gene classes are related to the metabolisms of xenobiotics, cofactors, vitamins and amino acids, and this divergence may underlie the different biological characteristics between the two species of whiteflies. Genes of differential expression between the two species are enriched in carbohydrate metabolism and regulation of autophagy. These findings provide molecular clues to uncover the biological and molecular differences between the two species of whiteflies.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0237744PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447059PMC
October 2020

Evolutionary Patterns of Sex-Biased Genes in Three Species of Haplodiploid Insects.

Insects 2020 May 26;11(6). Epub 2020 May 26.

Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

Females and males often differ obviously in morphology and behavior, and the differences between sexes are the result of natural selection and/or sexual selection. To a great extent, the differences between the two sexes are the result of differential gene expression. In haplodiploid insects, this phenomenon is obvious, since males develop from unfertilized zygotes and females develop from fertilized zygotes. Whiteflies of the species complex are typical haplodiploid insects, and some species of this complex are important pests of many crops worldwide. Here, we report the transcriptome profiles of males and females in three species of this whitefly complex. Between-species comparisons revealed that non-sex-biased genes display higher variation than male-biased or female-biased genes. Sex-biased genes evolve at a slow rate in protein coding sequences and gene expression and have a pattern of evolution that differs from those of social haplodiploid insects and diploid animals. Genes with high evolutionary rates are more related to non-sex-biased traits-such as nutrition, immune system, and detoxification-than to sex-biased traits, indicating that the evolution of protein coding sequences and gene expression has been mainly driven by non-sex-biased traits.
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http://dx.doi.org/10.3390/insects11060326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349267PMC
May 2020

Improved protein purification system based on C-terminal cleavage of Npu DnaE split intein.

Bioprocess Biosyst Eng 2020 Nov 23;43(11):1931-1941. Epub 2020 May 23.

Department of Chemical and Biomolecular Engineering, Ohio State University, Columbus, OH, 43210, USA.

A purification system was constructed with the N-segment of the Npu DnaE split intein as an affinity ligand immobilized onto an epoxy-activated medium and the C-segment used as the cleavable tag fusing target protein. The affinity properties of C-tagged proteins adsorbed on I affinity chromatography medium were studied with GFP as a model target protein. The saturated adsorption capacity and dynamic adsorption capacity reached 51.9-21.0 mg mL, respectively. With this system, two model proteins, GFP and alcohol dehydrogenase (ADH), has been successfully taglessly purified with regulation of Zn and DTT. The yield, purification factor and purity of purified tagless GFP reached 39, 11.7 and 97%, respectively; while these values for purified tagless ADH were 38.2, 6.8 and 91%, respectively. These results showed that the system for Npu DnaE split intein-mediated affinity adsorption and in situ cleavage is a potential platform for recombinant protein production.
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http://dx.doi.org/10.1007/s00449-020-02382-wDOI Listing
November 2020

Influence of Soil Properties and Aging on Antimony Toxicity for Barley Root Elongation.

Bull Environ Contam Toxicol 2020 May 8;104(5):714-720. Epub 2020 Apr 8.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

The study explored the Sb toxicity by investigating the impacts of 10% and 20% effective concentrations (EC10 and EC20, respectively) of Sb on the inhibition of barley root elongation in 21 Chinese soils with a wide range of physicochemical properties after aging for 3 months. The results demonstrated that various soil properties profoundly influenced the Sb toxicity which was ranged from 201-2506 mg Sb kg to 323-2973 mg Sb kg under EC10 and EC20, respectively. Soil sand fraction was a significant soil factor responsible for elevating Sb bioavailability. The bioavailable Sb concentration accounted for 2.08%-11.94% of total Sb content in all 21 soil samples and the decreased Sb bioavailability in this study was attributed to soil properties including soil clay fraction, amorphous and crystalloid iron, and oxides of manganese and aluminum. The findings would contribute in developing Sb toxicity threshold for establishing standard for Sb regulation in crop production.
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http://dx.doi.org/10.1007/s00128-020-02826-9DOI Listing
May 2020

Anteromedial thalamic nucleus to anterior cingulate cortex inputs modulate histaminergic itch sensation.

Neuropharmacology 2020 05 6;168:108028. Epub 2020 Mar 6.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica and Collaborative Innovation Center for Brain Science, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China; Zhejiang Chinese Medical University, Hangzhou, 310053, China.

Itch is an unpleasant feeling that triggers scratching behavior. Much progress has been made in identifying the mechanism of itch at the peripheral and spinal levels, however, itch circuits in the brain remain largely unexplored. We previously found that anterior cingulate cortex (ACC) to dorsal medial striatum (DMS) inputs modulated histamine-induced itch sensation, but how itch information was transmitted to ACC remained unclear. Here, we demonstrated that the anteromedial thalamic nucleus (AM) was activated during histaminergic itch, and there existed reciprocal neuronal projections between AM and ACC. Disconnection between AM and ACC resulted in a significant reduction of histaminergic, but not nonhistaminergic, itch-related scratching behavior. Optogenetic activation of AM-ACC, but not ACC-AM, projections evoked histaminergic itch sensation. Thus, our studies firstly reveal that AM is critical for histaminergic itch sensation and AM-ACC projections modulate histaminergic itch-induced scratching behavior.
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http://dx.doi.org/10.1016/j.neuropharm.2020.108028DOI Listing
May 2020

Time-dependent evolution of Zn(II) fractions in soils remediated by wheat straw biochar.

Sci Total Environ 2020 May 31;717:137021. Epub 2020 Jan 31.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China,. Electronic address:

Biochar is a cost-effective and multifunctional carbon material, which can be used to immobilize heavy metal (HM) in soil. To date, the immobilization of different HM by various biochars are well-studied, however, little is known about the release condition of the immobilized HM. As the released HM may bring a threat to the soil environment, it is critical to understand the release pattern of biochar-sorbed HM in soil. Herein, six wheat straw-derived biochars (WBs) pyrolyzed under different temperature and duration time were loaded with zinc(Zn (II)), and the evolution of Zn(II) fractions in soils remediated by WBs over time was investigated by Community Bureau of Reference (BCR) three-step sequential extraction method. The main Zn(II) species sorbed on WBs were the Zn(II) sorbed on the acidic functional groups of WB and that sorbed on WB surface via electrostatic interaction. Generally, Zn(II) sorbed on high-temperature WB was more mobile than that sorbed on low-temperature WB. In the red soil, the soluble and exchangeable Zn(II) (i.e., Zn(II) in Fraction 1) in WB was inclined to transform to organic matter associated-Zn(II) (i.e., Zn(II) in Fraction 3) and residual Zn(II) (i.e., Zn(II) in Fraction 4). In the yellow-brown soil, the soluble and exchangeable Zn(II) in WB was prone to convert into amorphous Fe/Mn oxide associated-Zn(II) (i.e., Zn(II) in Fraction 2) and residual Zn(II). These results imply that Zn(II) sorbed by WB has the risk to be released into the soil environment, and WB produced at low temperature are more suitable to remediate soils with low/neutral pH.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137021DOI Listing
May 2020

Diagnostic value of contrast-enhanced ultrasonography for intrahepatic cholangiocarcinoma with tumor diameter larger than 5 cm.

Hepatobiliary Pancreat Dis Int 2020 Jun 15;19(3):284-287. Epub 2020 Jan 15.

Department of Ultrasound Medicine, the First Affliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

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http://dx.doi.org/10.1016/j.hbpd.2020.01.001DOI Listing
June 2020

Genome-Wide Identification and Transcriptomic Analysis of MicroRNAs Across Various Amphioxus Organs Using Deep Sequencing.

Front Genet 2019 26;10:877. Epub 2019 Sep 26.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, China.

Amphioxus is the closest living invertebrate proxy of the vertebrate ancestor. Systematic gene identification and expression profile analysis of amphioxus organs are thus important for clarifying the molecular mechanisms of organ function formation and further understanding the evolutionary origin of organs and genes in vertebrates. The precise regulation of microRNAs (miRNAs) is crucial for the functional specification and differentiation of organs. In particular, those miRNAs that are expressed specifically in organs (OSMs) play key roles in organ identity, differentiation, and function. In this study, the genome-wide miRNA transcriptome was analyzed in eight organs of adult amphioxus using deep sequencing. A total of 167 known miRNAs and 23 novel miRNAs (named novel_mir), including 139 conserved miRNAs, were discovered, and 79 of these were identified as OSMs. Additionally, analyses of the expression patterns of eight randomly selected known miRNAs demonstrated the accuracy of the miRNA deep sequencing that was used in this study. Furthermore, potentially OSM-regulated genes were predicted for each organ type. Functional enrichment of these predicted targets, as well as further functional analyses of known OSMs, was conducted. We found that the OSMs were potentially to be involved in organ-specific functions, such as epidermis development, gonad development, muscle cell development, proteolysis, lipid metabolism, and generation of neurons. Moreover, OSMs with non-organ-specific functions were detected and primarily include those related to innate immunity and response to stimuli. These findings provide insights into the regulatory roles of OSMs in various amphioxus organs.
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http://dx.doi.org/10.3389/fgene.2019.00877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775235PMC
September 2019

Ube2b-dependent degradation of DNMT3a relieves a transcriptional brake on opiate-induced synaptic and behavioral plasticity.

Mol Psychiatry 2021 04 1;26(4):1162-1177. Epub 2019 Oct 1.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Compelling evidence suggests that synaptic structural plasticity, driven by remodeling of the actin cytoskeleton, underlies addictive drugs-induced long-lasting behavioral plasticity. However, the signaling mechanisms leading to actin cytoskeleton remodeling remain poorly defined. DNA methylation is a critical mechanism used to control activity-dependent gene expression essential for long-lasting synaptic plasticity. Here, we provide evidence that DNA methyltransferase DNMT3a is degraded by the E2 ubiquitin-conjugating enzyme Ube2b-mediated ubiquitination in dorsal hippocampus (DH) of rats that repeatedly self-administrated heroin. DNMT3a degradation leads to demethylation in CaMKK1 gene promotor, thereby facilitating CaMKK1 expression and consequent activation of its downstream target CaMKIα, an essential regulator of spinogenesis. CaMKK1/CaMKIα signaling regulates actin cytoskeleton remodeling in the DH and behavioral plasticity by activation of Rac1 via acting Rac guanine-nucleotide-exchange factor βPIX. These data suggest that Ube2b-dependent degradation of DNMT3a relieves a transcriptional brake on CaMKK1 gene and thus activates CaMKK1/CaMKIα/βPIX/Rac1 cascade, leading to drug use-induced actin polymerization and behavior plasticity.
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http://dx.doi.org/10.1038/s41380-019-0533-yDOI Listing
April 2021

Speciation and location of arsenic and antimony in rice samples around antimony mining area.

Environ Pollut 2019 Sep 22;252(Pt B):1439-1447. Epub 2019 Jun 22.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Arsenic (As) and antimony (Sb) are considered as priority environmental pollutants and their accumulation in crop plants particularly in rice has posed a great health risk. This study endeavored to investigate As and Sb contents in paired soil-rice samples obtained from Xikuangshan, the world largest active Sb mining region, situated in China, and to investigate As speciation and location in rice grains. The soil and rice samples were analyzed by coupling the wet chemistry, laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS), synchrotron-based micro X-ray fluorescence mapping (μ-XRF) and micro X-ray absorption near-edge structure (μ-XANES) spectroscopy. The results of field survey indicated that the paddy soil in the region was co-polluted by Sb (5.91-322.35 mg kg) and As (0.01-57.21 mg kg). Despite the higher Sb concentration in the soil, rice accumulated more As than Sb indicating the higher phytoavailability of As. Dimethylarsinic acid (DMA) was the predominant species (>60% on average) in the rice grains while the percentage of inorganic As species was 19%-63%. The μ-XRF mapping of the grain section revealed that the most of As was distributed and concentrated in rice husk, bran and embryo. Sb was distributed similarly to As but was not in the endosperm of rice grain based on LA-ICP-MS. The present results deepened our understanding of the As/Sb co-pollution and their association with the agricultural-product safety in the vicinity of Sb mining area.
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http://dx.doi.org/10.1016/j.envpol.2019.06.083DOI Listing
September 2019

The Adsorption Behavior of Atrazine in Common Soils in Northeast China.

Bull Environ Contam Toxicol 2019 Aug 1;103(2):316-322. Epub 2019 Jul 1.

College of Resources and Environment, Jilin Agricultural University, Changchun, 130118, China.

The objective of this study was to evaluate the adsorption capacity of atrazine and the effects of different environmental conditions such as temperature, pH, Ca and biochar on the adsorption characteristics of atrazine in different types of soil using the intermittent adsorption method. The kinetic experiment showed that the adsorption of atrazine in albic, black and saline-alkaline soils reached equilibrium within 24 h. In the thermodynamics experiment, the Freundlich model effectively described the adsorption characteristics of atrazine in all three types of soil, indicating that the adsorption process forms multi-molecular layers. Lower soil pH conditions were more favorable for the absorption of atrazine. The addition of appropriate concentrations of Ca or biochar could promote the adsorption of atrazine by the soil. Biochar could promote the fixation of atrazine in soils.
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http://dx.doi.org/10.1007/s00128-019-02671-5DOI Listing
August 2019

Cd(II) retention and remobilization on δ-MnO and Mn(III)-rich δ-MnO affected by Mn(II).

Environ Int 2019 09 22;130:104932. Epub 2019 Jun 22.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address:

Birnessite owing to its negative surface charge and defective structure exhibits high sorption affinities for Cd(II). However, Mn(II) can not only compete for the sorption sites with Cd(II), but also react with structural Mn(IV) in birnessite to form Mn(III), and thus, affect Cd(II) immobilization by birnessite. Herein, we investigate effects of Mn(II) on Cd(II) retention and remobilization on two birnessite δ-MnO and Mn(III)-rich δ-MnO (denoted as HE-MnO). At pH 5.5, Cd(II) sorption to birnessite was inhibited by Mn(II) addition. Mn(II) addition to δ-MnO led to Cd(II) migration from vacant sites to edge sites, forming double-corner sharing (DCS) complexes. Mn(II) introduction to δ-MnO led to less stable Cd(II) species formed on birnessite, indicating that Cd(II) was more firmly bound to vacant sites than edge sites of birnessite. Cd(II) formed double-edge sharing (DES) and DCS complexes on HE-MnO. Mn(II) addition to HE-MnO increased the CdMn distance in DES complexes. The stability of adsorbed Cd(II) on HE-MnO was slightly elevated due to Mn(II) addition. At pH 7.5, Mn(II) had no effect on Cd(II) sorption and desorption amounts on birnessite. However, low concentration of Mn(II) added to δ-MnO induced partial migration of Cd(II) from vacant sites to edge sites while high concentration of Mn(II) added to birnessite led to the formation of amorphous Cd(II)-Mn(III) coprecipitate. These findings imply that aqueous Mn(II) is an important factor in influencing Cd(II) immobilization by birnessite in the environment.
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http://dx.doi.org/10.1016/j.envint.2019.104932DOI Listing
September 2019

Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal.

Behav Brain Res 2019 10 4;372:112008. Epub 2019 Jun 4.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica and Collaborative Innovation Center for Brain Science, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China. Electronic address:

Addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations, encoded by changes of gene expression. We previously demonstrated that the changes in synaptic plasticity were required for the formation of aversive memories associated with morphine withdrawal. However, the proteins involved in synaptic plasticity and aversive memory formation have not been well explored. In the present study, we employed a two-dimensional gel electrophoresis (2-DE)-based proteomic technique to detect the changes of protein expression in the nucleus accumbens, amygdala and dorsal hippocampus of the rats that had developed conditioned morphine withdrawal. We found that twenty-three proteins were significantly altered in the amygdala and dorsal hippocampus after conditioned morphine withdrawal. These proteins can be classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and protein metabolism according to their biological functions. Eight proteins related to synaptic plasticity were further confirmed by western blot analysis. It is very likely that these identified proteins may contribute to conditioned morphine withdrawal-induced neural plasticity and aversive memory formation. Thus, our work will help understand the potential mechanism associated with generation of drug withdrawal memories.
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http://dx.doi.org/10.1016/j.bbr.2019.112008DOI Listing
October 2019

Antimony oxidation and sorption behavior on birnessites with different properties (δ-MnO and triclinic birnessite).

Environ Pollut 2019 Mar 4;246:990-998. Epub 2019 Jan 4.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, The Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address:

Birnessites are abundant naturally occurring minerals with high sorption and oxidation capacity that could therefore play an important role in antimony (Sb) migration and transformation. There are various types of birnessites in the environment. However, little is known about the similarities and differences in Sb oxidation and sorption on birnessites with different properties. In this study, the behavior of Sb oxidation and sorption on two contrasting birnessites (δ-MnO and triclinic birnessite (TrBir)) were investigated via batch and kinetic experiments and various spectroscopic techniques. Our results showed that the reaction mechanisms between Sb and the two birnessites were similar. The edge sites of birnessites were responsible for Sb(III) oxidation. Mn(IV) was reduced to Mn(III) and Mn(II), bound with birnessites and released to the solution, respectively. Because of the rapid rate of electron transfer of adsorbed Sb(III) to birnessites, the only Sb species on δ-MnO after the oxidation reaction was Sb(V). Sb(V) was adsorbed at the edge sites of birnessites by replacing the OH group of birnessites, forming corner-sharing complexes with birnessites. However, the Sb sorption and oxidation capacities of the two birnessites were significantly different. Poorly-crystallized δ-MnO exhibited a much higher oxidation and sorption capacity than well-crystallized TrBir because the former had many more edge sites than the latter. This study reveals the general mechanism of the reaction between Sb and birnessite and indicates that birnessite with a high number of edge sites would exhibit a huge capacity in Sb oxidation and sorption.
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http://dx.doi.org/10.1016/j.envpol.2018.12.027DOI Listing
March 2019

Author Correction: Draft genomic and transcriptome resources for marine chelicerate Tachypleus tridentatus.

Sci Data 2019 03 26;6(1):11. Epub 2019 Mar 26.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou, 53501, Guangxi, China.

In the original version of this Data Descriptor the word "Gulf" was incorrectly spelled in the affiliation "Ocean College, Beibu Gulf University, Qinzhou, 535011, Guangxi, China". This has now been corrected in both the HTML and PDF versions.
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http://dx.doi.org/10.1038/s41597-019-0025-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437645PMC
March 2019

Draft genomic and transcriptome resources for marine chelicerate Tachypleus tridentatus.

Sci Data 2019 02 26;6:190029. Epub 2019 Feb 26.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou, 53501 Guangxi, China.

Chinese horseshoe crabs (Tachypleus tridentatus), ancient marine arthropods dating back to the mid-Palaeozoic Era, have provided valuable resources for the detection of bacterial or fungal contamination. However, excessive exploitation for the amoebocyte lysate of Tachypleus has dramatically decreased the population of the Chinese horseshoe crabs. Thus, we present sequencing, assembly and annotation of T. tridentatus, with the hope of understanding the genomic feature of the living fossil and assisting scientists with the protection of this endangered species. The final genome contained a total size of 1.943 Gb, covering 90.23% of the estimated genome size. The transcriptome of three larval stages was constructed to investigate the candidate gene involved in the larval development and validate annotation. The completeness of the genome and gene models was estimated by BUSCO, reaching 96.2% and 95.4%, respectively. The synonymous substitution distribution of paralogues revealed that T. tridentatus had undergone two rounds of whole-genome duplication. All genomic and transcriptome data have been deposited in public databases, ready to be used by researchers working on horseshoe crabs.
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http://dx.doi.org/10.1038/sdata.2019.29DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390705PMC
February 2019

Genome-wide gene expression analysis reveals novel insights into the response to nitrite stress in gills of Branchiostoma belcheri.

Chemosphere 2019 Mar 26;218:609-615. Epub 2018 Nov 26.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500 China. Electronic address:

Amphioxus has been widely used as a model for the comparative immunology of vertebrates. Studies have reported that gene expression changes in the amphioxus gill in response to biotic stress, such as microbial and their mimic challenge, but little is known about how gene expression is affected by abiotic stress in the marine environment, such as nitrite. A lack of information regarding gene expression response to abiotic stress hinders a comprehensive understanding of gill defense response in amphioxus. Here, RNA sequencing was used to carry out gene expression profiling analyses of Branchiostoma belcheri gills under nitrite stress. Six libraries were created for the control and treatment groups, including three biological replicates. In total, 2416 differently expressed genes (DEGs) were detected in response to nitrite stress, of which 1522 DEGs were up-regulated in the treatment group in comparison to the control, while the remaining 894 DEGs were down-regulated genes. Functional enrichment revealed that these DEGs are primarily involved in disease, innate immunity, xenobiotic biodegradation and metabolism, and biomolecular processes and apoptosis. We screened 11 key nitrite-responsive DEGs to detect their expression responses to nitrite stress at different time points, and validate the sequencing data using real time quantitative PCR. The results indicated that the expression of gene encoding CYP3A, POD, CASPR1, GST, MAO, DDH, and XDH/XO were induced, while those encoding MRC, GT, DNASE1L, and RIPK5 were reduced, to participate in the anti-nitrite response. This study provides a useful resource for research of molecular toxicology in amphioxus under environmental stress.
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http://dx.doi.org/10.1016/j.chemosphere.2018.11.156DOI Listing
March 2019

Testosterone-retinoic acid signaling directs spermatogonial differentiation and seasonal spermatogenesis in the Plateau pika (Ochotona curzoniae).

Theriogenology 2019 Jan 29;123:74-82. Epub 2018 Sep 29.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810001, China; Qinghai Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810001, China. Electronic address:

During evolution, animals optimize their reproductive strategies to increase offspring survival. Seasonal breeders reproduce only during certain times of the year. In mammals, reproduction is tightly controlled by hypothalamus-pituitary-gonad axis. Although pathways regulating gametogenesis in non-seasonal model species have been well established, molecular insights into seasonal reproduction are severely limited. Using the Plateau pika (Ochotona curzoniae), a small rodent animal species native to the Qinghai-Tibetan plateau, as a model, here we report that seasonal spermatogenesis is governed at the level of spermatogonial differentiation. In testis of the reproductively dormant animals, undifferentiated spermatogonia failed to differentiate and accumulated in the seminiferous tubules. RNA-seq analyses of the active and dormant testes revealed that genes modulating retinoic acid biogenesis and steriodogenesis were differentially regulated. A single injection of all-trans retinoic acid (ATRA) reinitiated spermatogenesis and inhibition the function of RA-degrading enzyme CYP26B1 for 10 days induced spermatogonial differentiation. Strikingly, testosterone injection reinitiated spermatogenesis in short day adapted animals. Testosterone provides a permissive environment of RA biogenesis and actions in testis, therefore, indirectly controls spermatogonial differentiation. Collectively, these findings provide a key mechanistic insight regarding the molecular regulation of seasonal reproduction in mammals.
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http://dx.doi.org/10.1016/j.theriogenology.2018.09.033DOI Listing
January 2019

Screening of wheat straw biochars for the remediation of soils polluted with Zn (II) and Cd (II).

J Hazard Mater 2019 01 13;362:311-317. Epub 2018 Sep 13.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address:

The immobilization behaviors of Zn(II) and Cd(II) by wheat straw (WS) biochars could vary with the soil conditions. In the acidic environment, WS biochars produced at low temperature were more competent than those produced at high temperature on Zn(II) and Cd(II) immobilization; while WS biochars produced at high temperature were more effective than those produced at low temperature in the alkaline environment. The ions in the porewater could compromise the sorption capacities of Zn(II) and Cd(II) by WS biochars in acidic soils, while could enhance them in alkaline soils. For biochars produced at the same temperature, residence time had little effect on their behaviors of Zn(II) and Cd(II) immobilization. Only a small portion of immobilized Zn(II)/Cd(II) could be released from WS biochar in the simulated acid rain. Compared with Zn(II)/Cd(II) adsorbed on the acidic functional groups, Zn(II)/Cd(II) precipitates were more stable in 0.01 M CaCl solution. Most of the Zn(II) and Cd(II) species on biochar could be released in 1 mM citric acid solution. The immobilized Zn(II) and Cd(II) on WS biochar are likely to be released into the soil environment in the long run.
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http://dx.doi.org/10.1016/j.jhazmat.2018.09.034DOI Listing
January 2019

Pharmacological Characterization of Dezocine, a Potent Analgesic Acting as a κ Partial Agonist and μ Partial Agonist.

Sci Rep 2018 09 20;8(1):14087. Epub 2018 Sep 20.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica and Collaborative Innovation Center for Brain Science, Chinese Academy of Science, Shanghai, 201203, China.

Dezocine is becoming dominated in China market for relieving moderate to severe pain. It is believed that Dezocine's clinical efficacy and little chance to provoke adverse events during the therapeutic process are mainly attributed to its partial agonist activity at the μ opioid receptor. In the present work, we comprehensively studied the pharmacological characterization of Dezocine and identified that the analgesic effect of Dezocine was a result of action at both the κ and μ opioid receptors. We firstly found that Dezocine displayed preferential binding to μ opioid receptor over κ and δ opioid receptors. Dezocine, on its own, weakly stimulated G protein activation in cells expressing κ and μ receptors, but in the presence of full κ agonist U50,488 H and μ agonist DAMGO, Dezocine inhibited U50,488H- and DAMGO-mediated G protein activation, indicating that Dezocine was a κ partial agonist and μ partial agonist. Then the in intro results were verified by in vivo studies in mice. We observed that Dezocine-produced antinociception was significantly inhibited by κ antagonist nor-BNI and μ antagonist β-FNA pretreatment, indicating that Dezocine-mediated antinociception was via both the κ and μ opioid receptors. When co-administrating of Dezocine with U50,488 H or morphine, Dezocine was capable of inhibiting U50,488H- or morphine-induced antinociception. Finally, κ receptor activation-associated side effect sedation was investigated. We found that Dezocine displayed limited sedative effect with a ceiling effecting at a moderate dose. Thus, our work led to a better understanding of the analgesic mechanism of action of Dezocine in vivo.
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http://dx.doi.org/10.1038/s41598-018-32568-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148296PMC
September 2018

7β-Methyl substituent is a structural locus associated with activity cliff for nepenthone analogues.

Bioorg Med Chem 2018 08 24;26(14):4254-4263. Epub 2018 Jul 24.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China. Electronic address:

With the purpose of identifying novel selective κ opioid receptor (KOR) antagonists as potential antidepressants from nepenthone analogues, starting from N-nor-N-cyclopropylmethyl-nepenthone (SLL-020ACP), a highly selective and potent KOR agonist, a series of 7β-methyl-nepenthone analogues was conceived, synthesized and assayed on opioid receptors based on the concept of hybridization. According to the pharmacological results, the functional reversal observed in orvinol analogues by introduction of 7β-methyl substituent could not be reproduced in nepenthone analogues. Alternatively, introduction of 7β-methyl substituent was associated with substantial loss of both subtype selectivity and potency but not efficacy for nepenthone analogues, which was not found in 7β-methyl orvinol analogues. Surprisingly, SLL-603, a 7β-methyl analogue of SLL-020ACP, was identified to be a KOR full agonist. The possible molecular mechanism for the heterogeneity in activity cliff was also investigated. In conclusion, 7β-methyl substituent was a structural locus associated with activity cliff and demonstrated as a pharmacological heterogeneity between nepenthone and orvinol analogues that warrants further investigations.
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http://dx.doi.org/10.1016/j.bmc.2018.07.020DOI Listing
August 2018
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