Publications by authors named "Yu-Ting Zhang"

63 Publications

Dorsal raphe nucleus to anterior cingulate cortex 5-HTergic neural circuit modulates consolation and sociability.

Elife 2021 Jun 3;10. Epub 2021 Jun 3.

Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi'an, China.

Consolation is a common response to the distress of others in humans and some social animals, but the neural mechanisms underlying this behavior are not well characterized. By using socially monogamous mandarin voles, we found that optogenetic or chemogenetic inhibition of 5-HTergic neurons in the dorsal raphe nucleus (DR) or optogenetic inhibition of serotonin (5-HT) terminals in the anterior cingulate cortex (ACC) significantly decreased allogrooming time in the consolation test and reduced sociability in the three-chamber test. The release of 5-HT within the ACC and the activity of DR neurons were significantly increased during allogrooming, sniffing, and social approaching. Finally, we found that the activation of 5-HT1A receptors in the ACC was sufficient to reverse consolation and sociability deficits induced by the chemogenetic inhibition of 5-HTergic neurons in the DR. Our study provided the first direct evidence that DR-ACC 5-HTergic neural circuit is implicated in consolation-like behaviors and sociability.
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http://dx.doi.org/10.7554/eLife.67638DOI Listing
June 2021

Social Media Exposure, Psychological Distress, Emotion Regulation, and Depression During the COVID-19 Outbreak in Community Samples in China.

Front Psychiatry 2021 12;12:644899. Epub 2021 May 12.

Key Laboratory of Adolescent Cyberpsychology and Behavior Central China Normal University (CCNU), Ministry of Education, Wuhan, China.

The outbreak of coronavirus disease 2019 (COVID-19) has been a global emergency, affecting millions of individuals both physically and psychologically. The present research investigated the associations between social media exposure and depression during the COVID-19 outbreak by examining the mediating role of psychological distress and the moderating role of emotion regulation among members of the general public in China. Participants ( = 485) completed a set of questionnaires online, including demographic information, self-rated physical health, and social media exposure to topics related to COVID-19. The Impact of Event Scale-Revised (IES-R), the Beck Depression Inventory-II (BDI-II), and the Emotion Regulation Questionnaire (ERQ) were utilized to measure psychological distress about COVID-19, depression, and emotion regulation strategies, respectively. Results found that older age and greater levels of social media exposure were associated with more psychological distress about the virus ( = 0.14, = 0.003; = 0.22, < 0.001). Results of the moderated mediation model suggest that psychological distress mediated the relationship between social media exposure and depression (β = 0.10; = 0.07, 0.15). Furthermore, expressive suppression moderated the relationship between psychological distress and depression (β = 0.10, = 0.017). The findings are discussed in terms of the need for mental health assistance for individuals at high risk of depression, including the elderly and individuals who reported greater psychological distress and those who showed preference usage of suppression, during the COVID-19 crisis.
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http://dx.doi.org/10.3389/fpsyt.2021.644899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149733PMC
May 2021

Successful treatment of infantile hepatitis B with lamivudine: A case report.

World J Clin Cases 2021 May;9(14):3442-3448

Department of Infectious Disease, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China.

Background: How to treat infantile hepatitis B virus (HBV) infection remains a controversial issue. The nucleoside analogue lamivudine (LAM) has been approved to treat children (2 to 17 years old) with chronic hepatitis B. Here, we aimed to investigate the benefit of LAM treatment in infantile hepatitis B.

Case Summary: A 4-mo-old infant born to a hepatitis B surface antigen (HBsAg)-positive woman was found to be infected by HBV during a health checkup. Liver chemistry and HBV seromarker tests showed alanine aminotransferase of 106 U/L, HBsAg of 685.2 cut-off index, hepatitis B "e" antigen of 1454.0 cut-off index, and HBV DNA of > 1.0 × 10 IU/mL. LAM treatment (20 mg/d) was initiated, and after 19 mo, serum HBsAg was entirely cleared and hepatitis B surface antibody was present. The patient received LAM treatment for 2 years in total and has been followed for 3 years. During this period, serum hepatitis B surface antibody has been persistently positive, and serum HBV DNA was undetectable.

Conclusion: Early treatment of infantile hepatitis B with LAM could be safe and effective.
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http://dx.doi.org/10.12998/wjcc.v9.i14.3442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107904PMC
May 2021

Microglial MT1 activation inhibits LPS-induced neuroinflammation via regulation of metabolic reprogramming.

Aging Cell 2021 Jun 8;20(6):e13375. Epub 2021 May 8.

Department of Neurology, Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Although its pathogenesis remains unclear, a number of studies indicate that microglia-mediated neuroinflammation makes a great contribution to the pathogenesis of PD. Melatonin receptor 1 (MT1) is widely expressed in glia cells and neurons in substantia nigra (SN). Neuronal MT1 is a neuroprotective factor, but it remains largely unknown whether dysfunction of microglial MT1 is involved in the PD pathogenesis. Here, we found that MT1 was reduced in microglia of SN in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model. Microglial MT1 activation dramatically inhibited lipopolysaccharide (LPS)-induced neuroinflammation, whereas loss of microglial MT1 aggravated it. Metabolic reprogramming of microglia was found to contribute to the anti-inflammatory effects of MT1 activation. LPS-induced excessive aerobic glycolysis and impaired oxidative phosphorylation (OXPHOS) could be reversed by microglial MT1 activation. MT1 positively regulated pyruvate dehydrogenase alpha 1 (PDHA1) expression to enhance OXPHOS and suppress aerobic glycolysis. Furthermore, in LPS-treated microglia, MT1 activation decreased the toxicity of conditioned media to the dopaminergic (DA) cell line MES23.5. Most importantly, the anti-inflammatory effects of MT1 activation were observed in LPS-stimulated mouse model. In general, our study demonstrates that MT1 activation inhibits LPS-induced microglial activation through regulating its metabolic reprogramming, which provides a mechanistic insight for microglial MT1 in anti-inflammation.
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http://dx.doi.org/10.1111/acel.13375DOI Listing
June 2021

The Emergent Pelvic Artery Embolization in the Management of Postpartum Hemorrhage: A Systematic Review and Meta-analysis.

Obstet Gynecol Surv 2021 Apr;76(4):234-244

Assistant Professor, Department of Obstetrics and Gynecology, Shunde Hospital of Southern Medical University (The First People's Hospital of Shunde), Foshan, Guangdong, China.

Importance: Postpartum hemorrhage (PPH) is an emergent obstetric complication and the leading cause of maternal mortality. Pelvic arterial embolization (PAE) is an effective treatment for intractable PPH. However, a unique protocol has not been accepted in obstetrical practice.

Objective: To evaluate its efficiency, safety, complications, and outcomes, we conducted a systematic review and meta-analysis of PAE for PPH in the literature.

Evidence Acquisition: The Medline, the database of abstract of reviews, the index to allied health literature, and the Chinese database Sino-Med were searched on March 31, 2020, for studies on PAE for PPH. The data for PAE indication, agents, arteries, success rate, complications, and outcomes were extracted and syncretized for meta-analysis.

Results: From 1075 identified articles, 113 abstracts or full articles were retrieved and 43 studies were finally identified as meeting the including criteria. The results demonstrated that the indications for PAE were as follows: uterine atony, placental abnormality, delivery tract injury, disseminated intravascular coagulation, arteriovenous malformation, and vaginal hematoma. The embolization agents mostly in order were gelatin sponge particles, polyvinyl alcohol particles, Gelfoam, -butyl cyanoacrylate, microcoil, and glue; for arteries, they were mostly uterine artery and internal iliac artery. The clinical success rate was 90.5%, whereas the technical success rate was 99.3%. The most common complications of PAE were postembolization syndrome and menstrual abnormality.

Conclusions And Relevance: The emergent PAE is a safe and effective method with high success rate in life-threatening PPH management. Gelatin sponge granules measuring 500 to 1000 μm in diameter have safe results. Pelvic arterial embolization may affect the recovery of menses and increase PPH in the subsequent pregnancy, but there was no noted correlation with fetal growth restriction.
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http://dx.doi.org/10.1097/OGX.0000000000000887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081441PMC
April 2021

A Novel p.Tyr129His Variant in Leads to Dominant, Delayed-onset Hearing Loss with Possible Association with Congenital Anosmia.

Biomed Environ Sci 2021 Apr;34(4):314-318

Department of Otolaryngology-Head and Neck Surgery, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;Ear Institute, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai 200011, China.

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http://dx.doi.org/10.3967/bes2021.013DOI Listing
April 2021

[Effects of Water Management on Cadmium Accumulation by Rice ( L.) Growing in Typical Paddy Soil].

Huan Jing Ke Xue 2021 May;42(5):2512-2521

Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

In order to explore the effects of water management on the Cd accumulation of rice in paddy soils with different parent materials, a pot experiment with three paddy soils with different parent materials from Hunan Province (granite sandy soil, plate shale soil, and purple sandy shale soil) with different water management treatments [flooding and alternate wetting and drying (AWD)] was performed. The soil pH, DTPA-Cd, Fe plaque in the rice roots, and heavy metal concentration in the rice were determined. The results showed that the soil pH of the three paddy soils under the flooding treatment was increased by 0.17-1.33 units. During the filling and maturity periods, compared with that under AWD, the DTPA-Cd concentration in the three paddy soils was reduced by 14.39%-36.56% under the flooding treatment, but the DTPA-Fe concentration was increased by 35.35%-347.25%. In the three growth stages, the Cd and Mn concentrations in the Fe plaque (except for DCB-Fe) were in the order of tillering stage < filling stage < mature stage. Compared with that under AWD, the brown rice Cd concentration in the three soils was reduced by 57.84%-93.79% under flooding treatment. The Cd accumulation in rice was reduced under flooding treatment by reducing the DTPA-Cd via increasing the soil pH and DTPA-Fe and by decreasing the formation of Fe plaque. According to the results of the correlation and SEM analysis, the soil pH and DCB-Cd were the main factors affecting the Cd accumulation in rice grains, although the changes in the DTPA-Cd and DTPA-Fe also impacted the Cd in rice grains. In summary, our study demonstrated that water management had a significant impact on the Cd content in rice, and there were significant differences among the three paddy soils with different parent materials. In conclusion, the Cd content in rice grains was affected by the soil parent material, soil physicochemical properties, and Fe plaque on the surface of the rice roots. The granite sandy soil and plate shale soil with different water management treatments had significant impacts on the contents of heavy metals in rice. Continuous flooding is a valuable strategy for improving soil acidity and alkalinity and minimizing soil available Cd, but the soil parent materials must be considered.
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http://dx.doi.org/10.13227/j.hjkx.202008305DOI Listing
May 2021

Advances in intranasal application of stem cells in the treatment of central nervous system diseases.

Stem Cell Res Ther 2021 Mar 24;12(1):210. Epub 2021 Mar 24.

Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.

Stem cells are characterized by their self-renewal and multipotency and have great potential in the therapy of various disorders. However, the blood-brain barrier (BBB) limits the application of stem cells in the therapy of neurological disorders, especially in a noninvasive way. It has been shown that small molecular substances, macromolecular proteins, and even stem cells can bypass the BBB and reach the brain parenchyma following intranasal administration. Here, we review the possible brain-entry routes of transnasal treatment, the cell types, and diseases involved in intranasal stem cell therapy, and discuss its advantages and disadvantages in the treatment of central nervous system diseases, to provide a reference for the application of intranasal stem cell therapy.
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http://dx.doi.org/10.1186/s13287-021-02274-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992869PMC
March 2021

Depression Induced by Chronic Unpredictable Mild Stress Increases Susceptibility to Parkinson's Disease in Mice via Neuroinflammation Mediated by P2X7 Receptor.

ACS Chem Neurosci 2021 04 18;12(7):1262-1272. Epub 2021 Mar 18.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

The relationship between depression and Parkinson's disease (PD) is complicated and still not fully understood. We investigated whether depression increased the susceptibility to PD and whether this resulted from neuroinflammation mediated by purinergic ligand-gated ion channel 7 receptor (P2X7R) of microglia in mice. Depression was induced by a 14-day chronic unpredictable mild stress (CUMS), and PD was induced by 1-day acute injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Before MPTP administration, some mice were given brilliant blue G (BBG), a P2X7R inhibitor. Changes in depression and motor function were assessed by sucrose preference, tail suspension, open field, and rotating rod tests. Differences in P2X7R, caspase-1, NLRP3 inflammasome, interleukin (IL)-1β, tyrosine hydroxylase (TH), and microglial activation among experimental groups were detected by immunofluorescence, immunohistochemistry, western blotting, and ELISA. CUMS-induced depression-like behavior, and MPTP induced PD in mice. CUMS mice had no motor dysfunction, but the dyskinesia and loss of TH-positive neurons in the substantia nigra after MPTP treatment were more serious than with MPTP treatment alone. With behavioral changes, neuroinflammatory markers, such as caspase-1, NLRP3 and IL-1β increased, and microglia were activated as well as expression of P2X7R increased. Additionally, BBG partly reversed the above abnormalities. Summarily, we suggest that CUMS aggravates dyskinesia and death of dopaminergic neurons in an MPTP-PD model via promoting activation of microglia and neuroinflammation, which may be mediated by P2X7R. Inhibition of P2X7R could be a new control strategy for PD associated with depression.
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http://dx.doi.org/10.1021/acschemneuro.1c00095DOI Listing
April 2021

Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells.

Commun Biol 2021 Feb 15;4(1):204. Epub 2021 Feb 15.

Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands.

Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig cell-derived growth factor found in all vertebrates. Little information is available regarding Insl3 function in adult spermatogenesis. The Insl3 receptors Rxfp2a and 2b were expressed by type A spermatogonia and Sertoli and myoid cells, respectively, in zebrafish testis tissue. Loss of insl3 increased germ cell apoptosis in males starting at 9 months of age, but spermatogenesis appeared normal in fully fertile, younger adults. Insl3 changed the expression of 409 testicular genes. Among others, retinoic acid (RA) signaling was up- and peroxisome proliferator-activated receptor gamma (Pparg) signaling was down-regulated. Follow-up studies showed that RA and Pparg signaling mediated Insl3 effects, resulting in the increased production of differentiating spermatogonia. This suggests that Insl3 recruits two locally active nuclear receptor pathways to implement pituitary (Fsh) stimulation of spermatogenesis.
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http://dx.doi.org/10.1038/s42003-021-01708-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884674PMC
February 2021

The Effect of SPARC on the Proliferation and Migration of Limbal Epithelial Stem Cells During the Corneal Epithelial Wound Healing.

Stem Cells Dev 2021 Mar 1;30(6):301-308. Epub 2021 Mar 1.

Department of Ophthalmology, Cheeloo College of Medicine, Qilu Hospital, Shandong University, Jinan, P.R. China.

Secreted protein acidic and rich in cysteine (SPARC) shows a specific colocalization with limbal epithelial stem cells (LESCs) in vivo; however, the inherent relationship between SPARC and LESCs is still unclear. This study investigated the effects of SPARC on the maintenance of LESC stemness and corneal wound healing. To test the influence of different concentration of exogenous SPARC on the proliferation of LESCs, cell counting kit-8 assay and 5-ethynyl-2'-deoxyuridine staining were performed and the results indicated that 1 μg/mL SPARC was the optimum concentration for enhanced LESC proliferation. Compared with a control group, SPARC-treated group showed a higher expression of LESC-positive markers p63α, ABCG-2, and Bmi-1, and a lower level of differentiation marker cytokeratin-3 (CK3), thereby suggesting that SPARC could maintain LESC characteristic phenotype and suppress spontaneous epithelial differentiation in vitro. In vivo, exogenous SPARC accelerated the wound-healing process by both the enhancement of LESC proliferation and promoting the migration of the proliferating cells. However, the intact epithelium impaired this function of SPARC by contact inhibition.
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http://dx.doi.org/10.1089/scd.2020.0196DOI Listing
March 2021

Deficits in maintenance and interference control of working memory in major depression: evidence from the visuospatial change detection task.

Cogn Neuropsychiatry 2021 03 8;26(2):122-135. Epub 2021 Jan 8.

Neuropsychology and Applied Cognitive Neuroscience Laboratory, CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, People's Republic of China.

Introduction: Recent theories in cognitive psychology suggest that working memory (WM) processing involves a set of specific functions, in particular the WM functions of maintenance and interference control. Previous findings on WM impairments in patients with major depressive disorder (MDD) had been inconsistent, partly because earlier studies did not take into account these two different functions of WM.

Method: Forty-two participants with MDD and 39 controls completed the visuospatial change detection task. We estimated the WM function of maintenance, based on performance in trials using the targets only, and the WM function of interference control, based on performance in trials with distractor rectangles.

Results: Our results showed that participants with MDD had poorer WM function of maintenance and interference control than controls. However, the results of filtering efficiency did not show significant group difference, thus patients with MDD showed comparable impairments in WM function of maintanance as well as in WM function of interference control.

Conclusion: Our findings suggested that patients with MDD appear to show generalised impairments on visuospatial WM function of maintenance and interference control. Future studies should use refined paradigms to assess the different functions of WM and their distinctive contributions to symptomatology of depression.
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http://dx.doi.org/10.1080/13546805.2021.1871890DOI Listing
March 2021

Metabolomic differences of seminal plasma between boars with high and low average conception rates after artificial insemination.

Reprod Domest Anim 2021 Jan 23;56(1):161-171. Epub 2020 Nov 23.

Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, China.

Seminal plasma is a complex biological fluid containing many metabolites including amino acids, fructose, carbohydrates and lipids Metabolites play important roles in multiple biological processes, but details and significance of the seminal plasma metabolome related to boar fertility are unknown. The aim of the present study was to compare the comprehensive metabolome of seminal plasma from boars with different conception rate after artificial insemination and to identify the potential biomarkers. Semen samples were collected from boars which divided into two groups according to the conception rates in the offspring. Seminal plasma metabolites were isolated, purified, and then subjected to Ultra-high Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-qTOF-MS) procession. A total of 576 (Positive ion mode) and 377 (Negative ion mode) metabolites were identified in seminal plasma. Metabolites were identified and categorized according to their major chemical classes, including carboxylic acids and derivatives, organooxygen compounds, amino acids, peptides, and alogues, fatty amides, fatty acyls, benzene and substituted derivatives, purine nucleotides, pyrimidine nucleotides, glycosyl compounds, fatty acids and conjugates. The results showed that 4-Aminobenzoate, Pro-Asn, Ile-Tyr, Homoveratric acid and D-Biotin were higher in semen of boar with higher conception rate (HG) versus lower conception rate (LG) (p < .05), whereas L-Serine, Butoxyacetic acid, S-Methyl-5'-thioadenosine, Capsaicin and 1-O-(cis-9-Octadecenyl)-2-O-acetyl-sn-glycero-3-phosphocholine (PAF) were lower in HG than in LG (p < .05). These metabolites may be considered as candidate biomarkers for different fertility in boars.
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http://dx.doi.org/10.1111/rda.13861DOI Listing
January 2021

Microplastics decrease the toxicity of triphenyl phosphate (TPhP) in the marine medaka (Oryzias melastigma) larvae.

Sci Total Environ 2021 Apr 16;763:143040. Epub 2020 Oct 16.

Fujian Key Laboratory of Functional Marine Sensing Materials, Institute of Oceanography, Minjiang University, Fuzhou 350108, China. Electronic address:

Plastics have been recognized as a serious threat to the environment. Besides their own toxicity, microplastics can interact with other environmental pollutants, acting as carriers and potentially modulating their toxicity. In this study, the toxicity of polystyrene (PS) microplastic fragments (plain PS; carboxylated PS, PS-COOH and aminated PS, PS-NH) and triphenyl phosphate (TPhP) (an emerging organophosphate flame retardant) at the environmentally relevant concentrations to the marine medaka (Oryzias melastigma) larvae was investigated. Larvae were exposed to 20 μg/L of microplastic fragments or 20 and 100 μg/L of TPhP or a combination of both for 7 days. The results showed that the three microplastics did not affect the larval locomotor activity. For TPhP, the larval moving duration and distance moved were significantly decreased by the TPhP exposure, with a maximum decrease of 43.5% and 59.4% respectively. Exposure to 100 μg/L TPhP respectively down-regulated the expression levels of sine oculis homeobox homologue 3 (six3) and short wavelength-sensitive type 2 (sws2) by 19.1% and 41.7%, suggesting that TPhP might disturb eye development and photoreception and consequently the low locomotor activity in the larvae. Interestingly, during the binary mixture exposure, the presence of PS, PS-COOH or PS-NH reversed the low locomotor activity induced by 100 μg/L TPhP to the normal level. Relative to the larvae from the 100 μg/L TPhP group, the movement duration and distance moved were increased by approximately 60% and 100%, respectively, in the larvae from the TPhP + PS, TPhP + PS-COOH and TPhP + PS-NH groups. However, the gene expression profiles were distinct among the fish from the TPhP + PS, TPhP + PS-COOH and TPhP + PS-NH groups, implying different mechanisms underlying the reversal of the locomotor activity. The findings in this study challenge the general view that microplastics aggravate the toxicity of the adsorbed pollutants, and help better understand the environmental risk of microplastic pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143040DOI Listing
April 2021

Comparison of Clinical and Radiologic Outcome Between Mini-Open Wiltse Approach and Fluoroscopic-Guided Percutaneous Pedicle Screw Placement: A Randomized Controlled Trial.

World Neurosurg 2020 12 27;144:e368-e375. Epub 2020 Aug 27.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China. Electronic address:

Objective: To compare clinical efficacy, radiographic outcome, and radiation exposure between mini-open pedicle screw (MPS) fixation with the Wiltse approach and percutaneous pedicle screw (PPS) fixation in treatment of young and middle-aged patients with thoracolumbar burst fractures.

Methods: Of 60 patients with thoracolumbar vertebrae fractures treated in our hospital from January 2017 to January 2018, 30 were randomly assigned to the MPS group and 30 were randomly assigned to the PPS group. Clinical efficacy, radiographic outcome, and radiation exposure were compared between the 2 groups.

Results: The average age of patients was 42.2 ± 6.7 years in the MPS group and 43.0 ± 6.9 years in the PPS group (P = 0.668). There was no significant difference between the 2 groups in blood loss, hospital stay, postoperative visual analog scale score for back pain, and Oswestry Disability Index score. The vertebral body height and vertebral body angle of the MPS group were significantly better than those of the PPS group at the last follow-up. There was no significant difference in the accuracy rate of pedicle screw placement between the MPS group and the PPS group; the facet joint violation was significantly higher in the PPS group. The average radiation exposure dosage was lower in the MPS group.

Conclusions: Both MPS fixation with the Wiltse approach and PPS fixation are safe and effective in the treatment of single-segment thoracolumbar vertebral fractures. Nevertheless, considering the surgical duration, radiation exposure, facet joint violation, vertebral body height, and vertebral body angle at the last follow-up, MPS fixation with the Wiltse approach is a better choice than PPS.
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http://dx.doi.org/10.1016/j.wneu.2020.08.145DOI Listing
December 2020

Spontaneous resolution of a traumatic cataract in a patient with an open-globe ocular injury: a case report.

BMC Ophthalmol 2020 Jul 13;20(1):285. Epub 2020 Jul 13.

Department of Ophthalmology, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.

Background: We report a case of spontaneous resolution of a traumatic cataract in a patient with an open-globe ocular injury. This case highlights the importance of conservative management in these types of cases, as excellent visual outcome is possible without invasive surgical intervention.

Case Presentation: A 13-year-old boy presented with a corneal laceration in the left eye caused by a neuter pen. He underwent emergency repair of the corneal laceration under general anesthesia, and at 3 days post-op, a dense posterior cortical cataract was observed. Based on the patient's age and normal visual development, in addition to preserving accommodative potential, the patient received conservative management and follow-up. Interestingly, the cataract spontaneously resolved over the following 9 months and the corrected distance visual acuity in the injured eye was restored from finger counting at 50 cm, to 20/25 + 3.

Conclusions: To optimize treatment in pediatric traumatic cataract, several critical factors such as age, visual development and the preservation of accommodative potential, need to be comprehensively considered. Conservative management with lens preservation is important to consider in young, traumatic cataract patients where invasive surgical intervention may not be required.
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http://dx.doi.org/10.1186/s12886-020-01555-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359477PMC
July 2020

Long-Term Effects of Polyvinyl Chloride Microplastics on Anaerobic Granular Sludge for Recovering Methane from Wastewater.

Environ Sci Technol 2020 08 24;54(15):9662-9671. Epub 2020 Jul 24.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.

Polyvinyl chloride microplastics (PVC-MPs) are emerging contaminants affecting biological wastewater treatment processes. However, most of the previous studies mainly focused on their short-term impacts on floc sludge, with little work being conducted to explore their potential effects on more complex anaerobic granular sludge (AGS), which has been widely used for high-strength organic wastewater treatment. In this paper, the long-term effects of PVC-MPs on AGS were investigated continuous feeding tests that are representative of real wastewater treatment processes. The results of a continuous 264 days test showed that the prolonged exposure of PVC-MPs at 15-150 MPs·L significantly ( = 7.86 × 10, 3.44 × 10, and 5.29 × 10) inhibited the COD removal efficiency of AGS by 13.2%-35.5%, accompanied by a 11.0%-32.3% decreased production of methane and 40.3%-272.7% increased accumulation of short-chain fatty acids (SCFAs). In addition, the PVC-MPs exposure suppressed the secretion of extracellular polymeric substances (EPS), causing AGS and the inside microorganisms to lose the protection of EPS, thereby resulting in granule breakage and decreased cells viability. Aligning with the deteriorated performance, the long-term exposure of PVC-MPs reduced the total microbial populations and the relative abundances of key methanogens and acidogens. A toxicity mechanism assessment revealed that the negative impacts induced by PVC-MPs are mainly attributed to the toxic leachate and excess oxidative stress.
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http://dx.doi.org/10.1021/acs.est.0c02433DOI Listing
August 2020

Insights into the microbial response of anaerobic granular sludge during long-term exposure to polyethylene terephthalate microplastics.

Water Res 2020 Jul 3;179:115898. Epub 2020 May 3.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China. Electronic address:

The negative effects of ubiquitous microplastics on wastewater treatment have attracted increasing attention. However, the potential impacts of microplastics on anaerobic granular sludge (AGS) remain unknown. To fill this knowledge gap, this paper investigated the response of AGS to the exposure of model microplastics (polyethylene terephthalate (PET-MPs)) and provided insights into the mechanisms involved. The 84 days' long-term exposure experiments demonstrated that PET-MPs, at relatively low level (15 MP L) did not affect AGS performance during anaerobic wastewater treatment, while 75-300 MP L of PET-MPs caused the decreases of COD removal efficiency and methane yields by 17.4-30.4% and 17.2-28.4%, accompanied with the 119.4-227.8% increase in short-chain fatty acid (SCFA) accumulation and particle breakage. Extracellular polymeric substances (EPS) analysis showed that dosage-dependent tolerance of AGS to PET-MPs was attributed to the induced EPS producing protection role, but PET-MPs at higher concentrations (75-300 MP L) suppressed EPS generation. Correspondingly, microbial community analysis revealed that the populations of key acidogens (e.g., Levilinea sp.) and methanogens (e.g., Methanosaeta sp.) decreased after long-term exposure to PET-MPs. Assessment of the toxicity of PET-MPs revealed that the leached di-n-butyl phthalate (DBP) and the induced reactive oxygen species (ROS) by PET-MPs were causing toxicity towards AGS, confirmed by the increases in cell mortality and lactate dehydrogenase (LDH) release. These results provide novel insights into the ecological risk assessment of microplastics in anaerobic wastewater treatment system.
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http://dx.doi.org/10.1016/j.watres.2020.115898DOI Listing
July 2020

Preparation and antitumor effects of 4-amino-1,2,4-triazole Schiff base derivative.

J Int Med Res 2020 Feb;48(2):300060520903874

Clinical Research Center, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China.

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http://dx.doi.org/10.1177/0300060520903874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110913PMC
February 2020

Impact of Maternal Separation on Dopamine System and its Association with Parkinson's Disease.

Neuromolecular Med 2020 09 13;22(3):335-340. Epub 2020 Jan 13.

Institute of Neuroscience, Soochow University, Suzhou, 215000, Jiangsu, China.

As a type of stress, maternal separation (MS) has been one of the most widely used models in neuropsychiatric research. An increasing number of studies has found that MS not only affects the function of the hypothalamic-pituitary-adrenal axis and hippocampal 5-hydroxytryptamine system, but also causes dysfunction of the central dopamine (DA) system and increases the susceptibility of dopaminergic neurons to pathogenic factors of Parkinson's disease (PD), for instance, 6-hydroxydopamine, thus impairing motor function. We reviewed the impact of MS on the DA system and its correlation with PD and found the following: (1) discrepant effects of MS on the DA system have been reported; (2) MS is a good model to study the impact of stress on the occurrence and development of PD, however, unified modeling criteria of MS are required; (3) correlation between MS and PD may involve the impact of MS on the DA system, which however is not the only connection; (4) intervening measures can block pathways between MS and PD, which provides reference for the prevention of PD in specific populations such as left-behind children.
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http://dx.doi.org/10.1007/s12017-019-08587-xDOI Listing
September 2020

Expression of interleukin-32 in bone marrow of patients with myeloma and its prognostic significance.

World J Clin Cases 2019 Dec;7(24):4234-4244

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.

Background: The guiding effect of prognostic stratification in multiple myeloma (MM) for treatment has been increasingly emphasized in recent years. The stratification of risk factors based on the International Staging System (ISS), Durie-Salmon (DS) staging and related indicators is affected by the renal function of patients, resulting in poor performance. This study assesses the relationship between interleukin-32 (IL-32) and related risk factors in 67 patients with MM and their clinical outcomes.

Aim: To investigate the feasibility of IL-32 in evaluating prognosis in patients with MM and the factors influencing prognosis.

Methods: This was a pragmatic, prospective observational study of patients with MM at a single center. According to IL-32 level, patients were divided into two groups. The variables under consideration included age, blood β-microglobulin, albumin, C-reactive protein, serum calcium, serum creatinine, lactate dehydrogenase, M protein type, ISS stage, DS stage, and IL-32 levels and minimal residual disease (MRD) after induction treatment. The main outcomes were progression-free survival (PFS) and overall survival (OS).

Results: IL-32 was an important factor affecting PFS and OS in patients with MM. Compared with patients with IL-32 levels ≥ 856.4 pg/mL, patients with IL-32 levels < 856.4 pg/mL had longer PFS ( = 0.0387) and OS ( = 0.0379); Univariate analysis showed that IL-32 level and MRD were significantly associated with OS and PFS ( < 0.05). Multivariate analysis showed that IL-32 levels ≥ 856.4 pg/mL and MRD positive were still independent risk factors for OS and PFS ( < 0.05).

Conclusion: IL-32 is valuable for assessing the prognosis of MM patients. IL-32 level combined with MRD may be a useful routine evaluation index for MM patients after treatment.
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http://dx.doi.org/10.12998/wjcc.v7.i24.4234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940335PMC
December 2019

Improvement in the in vitro development of cloned pig embryos after kdm4a overexpression and an H3K9me3 methyltransferase inhibitor treatment.

Theriogenology 2020 Apr 25;146:162-170. Epub 2019 Nov 25.

Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, PR China. Electronic address:

Aberrant epigenetic reprogramming is a major cause of the developmental failure of embryos after somatic cell nuclear transfer (SCNT). Histone H3 lysine 9 trimethylation (H3K9me3), a histone marker of transcriptional repression, is considered a key barrier to the development of cloned embryos. In the present study, H3K9me3 levels were much higher in SCNT embryos than IVF embryos at the 4-cell and 2-cell stages. The microinjection of the kdm4a mRNA encoding an H3K9me3 demethylase significantly increased the developmental efficiency of cloned porcine embryos. Moreover, we evaluated the effect of chaetocin, an inhibitor of histone methyltransferases suv39h1/2, on SCNT embryo development. Chaetocin did not suppress the H3K9me3 modification in porcine embryonic fibroblast (PEF) but downregulated the expression of suv39h1, suv39h2, and kdm4d. However, 10 nM chaetocin treatment efficiently decreased the H3K9me3 level in cloned embryos. Importantly, a chaetocin treatment at the 4-cell stage for 6 h significantly increased the blastocyst rate and total cell numbers. Furthermore, the inhibitor treatment upregulated the expression of related developmental genes. In summary, both overexpression of kdm4a and treatment with a suv39h1/2 inhibitor improve the epigenetic reprogramming of cloned embryos and further improve the developmental competence in vitro.
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http://dx.doi.org/10.1016/j.theriogenology.2019.11.027DOI Listing
April 2020

3D shape measurement based on structured light field imaging.

Math Biosci Eng 2019 10;17(1):654-668

School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.

In this paper, a three-dimensional (3D) shape measurement method based on structured light field imaging is proposed, which contributes to the biomedical imaging. Generally, light field imaging is challenging to accomplish the 3D shape measurement accurately, as the slope estimation method based on radiance consistency is inaccurate. Taking into consideration the special modulation of structured light field, we utilize the phase information to substitute the phase consistency for the radiance consistency in epi-polar image (EPI) at first. Therefore, the 3D coordinates are derived after light field calibration, but the results are coarse due to slope estimation error and need to be corrected. Furthermore, the 3D coordinates refinement is performed based on relationship between the structured light field image and DMD image of the projector, which allows to improve the performance of the 3D shape measurement. The necessary light field camera calibration is described to generalize its application. Subsequently, the effectiveness of the proposed method is demonstrated with a sculpture and compared to the results of a conventional PMP system.
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http://dx.doi.org/10.3934/mbe.2020034DOI Listing
October 2019

SPARC promotes self-renewal of limbal epithelial stem cells and ocular surface restoration through JNK and p38-MAPK signaling pathways.

Stem Cells 2020 01 19;38(1):134-145. Epub 2019 Nov 19.

Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

The purpose of this study was to investigate the effects of secreted protein acidic and rich in cysteine (SPARC) on the maintenance of limbal epithelial stem cell (LESC) stemness and restoration of ocular surface. To determine the suitable concentration of SPARC for LESC culture, the marker expression, mitogenic effect, and holoclone-forming capacity of LESCs treated with different concentrations of SPARC were analyzed. To investigate the mechanism of SPARC's action on the preservation of LESCs stemness, the phosphorylation of related signaling pathways was evaluated by Western blotting. A corneal wound model was established to verify the function of SPARC in ocular surface repair. Consecutive subculturing, colony-forming efficiency, immunofluorescence, and 5-ethynyl-2-deoxyuridine incorporation assays indicated that 1 μg/mL SPARC was a suitable concentration to stimulate LESC proliferation and preserve their proliferative potential. Compared with a control group, 1 μg/mL SPARC effectively increased the expression of ABCG-2, Bmi-1, and Ki67, while decreasing that of CK3/12. The mitogenic effect of SPARC on LESCs was found to be mediated by the phosphorylation of c-Jun N-terminal kinase (JNK) and p38-MAPK signaling pathways, whereas the inhibitors of JNK and p38 MAPK reduced the marker expression and mitogenic capacity of LESCs. In a corneal injury model, SPARC facilitated corneal epithelial wound healing and promoted the proliferation of p63α-positive cells both in the limbus and in the epithelial healing front. SPARC promotes proliferation while suppressing spontaneous differentiation of LESCs through JNK and p38-MAPK signaling pathways, suggesting that SPARC is a promising factor for the improvement of LESCs culture in vitro and in vivo.
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http://dx.doi.org/10.1002/stem.3100DOI Listing
January 2020

Diminished Anticipatory and Consummatory Pleasure in Dysphoria: Evidence From an Experience Sampling Study.

Front Psychol 2019 19;10:2124. Epub 2019 Sep 19.

School of Psychology, Central China Normal University, Wuhan, China.

Anhedonia, the experience of diminished pleasure, is a core feature of major depressive disorder and is often present long before the diagnosis of depression. Most previous studies have investigated anhedonia with self-report measures of trait anhedonia or with behavioral paradigms using laboratory stimuli, and the real-time characteristics of hedonic processing in subclinical depression remain under-investigated. We used the experience sampling method to evaluate momentary experience of hedonic feelings in the context of daily life. Dysphoric ( = 49) and non-dysphoric ( = 51) college students completed assessments of their current positive affect (PA), as well as state anticipatory and consummatory pleasure, 3 or 4 times a day every day for 2 weeks. The results showed that dysphoric individuals reported less state anticipatory and consummatory pleasure compared with non-dysphoric individuals. Moreover, significant time-lagged associations between anticipatory pleasure and follow-up consummatory pleasure were found in the whole sample, after adjustment for current PA. The current findings thus hold considerable promise in advancing our understanding of anhedonia as well as the important role of state anticipatory pleasure in relation to depression.
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http://dx.doi.org/10.3389/fpsyg.2019.02124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761272PMC
September 2019

PGE2 inhibits spermatogonia differentiation in zebrafish: interaction with Fsh and an androgen.

J Endocrinol 2020 01;244(1):163-175

Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands.

Changes in zebrafish testicular gene expression induced by follicle-stimulating hormone (Fsh) or anti-Mullerian hormone (Amh) suggested that Amh inhibition and Fsh stimulation of spermatogenesis involved up and downregulation, respectively, of prostaglandin (PG) signaling. We found that Sertoli cells contacting type A undifferentiated (Aund) and differentiating (Adiff) spermatogonia expressed a key enzyme of PG production (Ptgs2); previous work showed that Sertoli cells contacting Adiff and B spermatogonia and spermatocytes showed ptges3b expression, an enzyme catalyzing PGE2 production. In primary testis tissue cultures, PGE2, but not PGD2 or PGF2α, reduced the mitotic activity of Adiff and their development into B spermatogonia. Vice versa, inhibiting PG production increased the mitotic activity of Adiff and B spermatogonia. Studies with pharmacological PG receptor antagonists suggest that an Ep4 receptor mediates the inhibitory effects on the development of spermatogonia, and cell-sorting experiments indicated this receptor is expressed mainly by testicular somatic cells. Combined inhibition of PG and steroid production moreover reduced the mitotic activity of Aund spermatogonia and led to their partial depletion, suggesting that androgens (and/or other testicular steroids), supported by PGE2, otherwise prevent depletion of Aund. Androgens also decreased testicular PGE2 production, increased the transcript levels of the enzyme-catabolizing PGs and decreased PGE2 receptor ptger4b transcript levels. Also Fsh potentially reduced, independent of androgens, PGE2 production by decreasing ptges3b transcript levels. Taken together, our results indicate that PGE2, via Ep4 receptors, favors self-renewal in conjunction with androgens and, independent of Fsh and androgens, inhibits differentiating divisions of spermatogonia.
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http://dx.doi.org/10.1530/JOE-19-0309DOI Listing
January 2020

[Congenital ectrodactyly caused by chromosome 10q24.31 duplication and its pathogenetic analysis].

Yi Chuan 2019 Aug;41(8):716-724

Department of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.

In order to investigate the genetic variations and the clinical manifestations of a range of congenital ectrodactyly family and to summarize the split hand/foot malformation (SHFM) types and their related pathogenic genes, we conducted phenotypic analyses of patient's limbs by physical and X-ray examination. The haplotypes were analyzed by using the extracted genes from peripheral blood on D10S1709, D10S192, D10S597, D10S1693 and D10S587 loci, and the mutation duplication loci were confirmed by Array-CGH detection. The pathogenic factors and inheritance pattern of SHFM were analyzed based on family investigation and gene analysis. Results demonstrate the proband's phenotype is typically of a congenital SHFM which is manifested by missing bilateral index and middle fingers, short bilateral thumbs, deformed left ring finger with webbing of the skin missing at the middle finger; bilateral big toe with the second and the third toe missing, fourth and fifth toe fusion leading to a deformed toe separated from the first toe by the middle of the foot. The haplotype analyses show that there is a repeat of at least 610 kb in chromosome 10q24.31-10q24.32 region. Array-CGH analysis shows 10q24.31 (102 832 650-103 511 083) ×3. Our results demonstrate that the pathogenic gene variation of ectrodactyly in this family is due to duplication of 10q24.31 (102 832 650~103 511 083). The haplotype 165-251-289-219-102 can be used as a disease marker for detecting 10q24.31~10q24.32 allele for SHFM.
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http://dx.doi.org/10.16288/j.yczz.19-125DOI Listing
August 2019

Polyethylene terephthalate microplastics affect hydrogen production from alkaline anaerobic fermentation of waste activated sludge through altering viability and activity of anaerobic microorganisms.

Water Res 2019 Oct 17;163:114881. Epub 2019 Jul 17.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China. Electronic address:

Alkaline (especially pH 10) anaerobic fermentation of waste activated sludge (WAS) has been reported to be an effective approach for hydrogen production through inhibiting the homoacetogenesis and methanogenesis. However, the potential effect of the widespread microplastics in sludge on the performance of hydrogen production has never been reported. To fill this knowledge gap, the dominant polyethylene terephthalate (PET) microplastics in WAS were selected as the model microplastics to evaluate their influences on hydrogen production during alkaline anaerobic fermentation of WAS as well as the key mechanisms involved. Experimental results demonstrated that hydrogen production from WAS decreased in the presence of PET microplastics (i.e., 10, 30 and 60 particles/g-TS) compared to the control, with the hydrogen yield at 60 particles/g-TS being only 70.7 ± 0.9% of the control. Although the hydrogen consumption (i.e., homoacetogenesis and methanogenesis) was restrained under alkaline (pH 10) condition, PET microplastics inhibited hydrolysis, acidogenesis and acetogenesis in alkaline WAS anaerobic fermentation, leading to the inhibitory effect on hydrogen production. This was further confirmed by the microbial analysis, which clearly showed PET microplastics caused the shift of the microbial community toward the direction against hydrolysis-acidification. Mechanism studies revealed that PET microplastics carried on their negative influence mainly through leaching the toxic di-n-butyl phthalate (DBP). The reactive oxygen species (ROS) and live/dead staining tests indicated that the increased ROS was induced by PET microplastics, causing more cells dead, which further resulted in the decreased production of hydrogen.
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http://dx.doi.org/10.1016/j.watres.2019.114881DOI Listing
October 2019

Dispersion of Poly(urea-formaldehyde)-Based Microcapsules for Self-Healing and Anticorrosion Applications.

Langmuir 2019 Jun 24;35(24):7871-7878. Epub 2019 May 24.

Institute of Materials Science and Engineering, and Department of Materials & Mineral Resources Engineering , National Taipei University of Technology , Box 2761, No. 1, Sec. 3, Zhongxiao E. Rd. , Taipei 10608 , Taiwan.

An effective dispersant, oleyl phosphate (OP), for the dispersion of poly(urea-formaldehyde)-based microcapsules in a typical epoxy coating material is proposed. Based on electron microscopy observations and rheological and mechanical characterizations, it is observed that the addition of merely 0.5 wt % of OP is sufficient to obtain good dispersion of the microcapsules in the epoxy. In the self-healing and anticorrosion experiments, a microcapsule content of at least 15 wt % is required to efficiently restore the epoxy matrix and provide corrosion protection to underlying low-carbon steel when the particles are not dispersed; however, the amount of microcapsules required to obtain good self-healing and anticorrosion efficiencies can be greatly reduced to only 5 wt % when the microcapsules are dispersed by OP.
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http://dx.doi.org/10.1021/acs.langmuir.9b00526DOI Listing
June 2019

Application of the CRISPR/Cas system for genome editing in microalgae.

Appl Microbiol Biotechnol 2019 Apr 15;103(8):3239-3248. Epub 2019 Mar 15.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.

Microalgae are arguably the most abundant single-celled eukaryotes and are widely distributed in oceans and freshwater lakes. Moreover, microalgae are widely used in biotechnology to produce bioenergy and high-value products such as polyunsaturated fatty acids (PUFAs), bioactive peptides, proteins, antioxidants and so on. In general, genetic editing techniques were adapted to increase the production of microalgal metabolites. The main genome editing tools available today include zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas nuclease system. Due to its high genome editing efficiency, the CRISPR/Cas system is emerging as the most important genome editing method. In this review, we summarized the available literature on the application of CRISPR/Cas in microalgal genetic engineering, including transformation methods, strategies for the expression of Cas9 and sgRNA, the CRISPR/Cas9-mediated gene knock-in/knock-out strategies, and CRISPR interference expression modification strategies.
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http://dx.doi.org/10.1007/s00253-019-09726-xDOI Listing
April 2019