Publications by authors named "Yu-Ling Li"

117 Publications

An Abdominal Registration Technology for Integration of Nanomaterial Imaging-Aided Diagnosis and Treatment.

J Biomed Nanotechnol 2021 May;17(5):952-959

School of Automation, Central South University, Changsha 410083, China.

Image registration technology is a key technology used in the process of nanomaterial imaging-aided diagnosis and targeted therapy effect monitoring for abdominal diseases. Recently, the deep-learning based methods have been increasingly used for large-scale medical image registration, because their iteration is much less than those of traditional ones. In this paper, a coarse-to-fine unsupervised learning-based three-dimensional (3D) abdominal CT image registration method is presented. Firstly, an affine transformation was used as an initial step to deal with large deformation between two images. Secondly, an unsupervised total loss function containing similarity, smoothness, and topology preservation measures was proposed to achieve better registration performances during convolutional neural network (CNN) training and testing. The experimental results demonstrated that the proposed method severally obtains the average MSE, PSNR, and SSIM values of 0.0055, 22.7950, and 0.8241, which outperformed some existing traditional and unsupervised learning-based methods. Moreover, our method can register 3D abdominal CT images with shortest time and is expected to become a real-time method for clinical application.
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http://dx.doi.org/10.1166/jbn.2021.3076DOI Listing
May 2021

Mu-opioid receptors in septum mediate the development of behavioural sensitization to a single morphine exposure in male rats.

Addict Biol 2021 May 24:e13066. Epub 2021 May 24.

Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Behavioural sensitization (BS) is characterized by enhanced psychomotor responses to a dose of substance of abuse after prior repeated exposure. We previously reported that BS can be induced by a single injection of morphine in rats, whereas septal nuclei are specifically involved in the development phase of BS. Here, we demonstrated that intra-LS or intra-MS microinjections also incubated BS to a systemic morphine injection in a cross-sensitization fashion, whereas inactivation of either subdivision of septal nuclei (LS: lateral septum; MS: medial septum) can negate this ability of morphine. Then, non-selective (naloxone) and selective (μ-, δ- and κ-)opioid receptor antagonists were directly delivered into LS or MS, respectively, ahead of a morphine microinjection, whereas only μ-opioid receptors in both LS and MS play indispensable roles in mediating the BS development. Finally, there was a pronounced elevation in the levels of the monoamines (i.e. dopamine, homovanillic acid, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid) in the septum, 8 h after a morphine injection detected with a HPLC-ECD method, suggesting that dopaminergi and serotoninergic systems are implicated in the BS formation. Our studies demonstrated that septal nuclei critically participate in the BS development. Essentially, μ- instead of δ- or κ-opioid receptors in LS and MS mediate sensitization to opiates.
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http://dx.doi.org/10.1111/adb.13066DOI Listing
May 2021

Neck circumference and waist circumference associated with cardiovascular events in type 2 diabetes (Beijing Community Diabetes Study 23).

Sci Rep 2021 May 4;11(1):9491. Epub 2021 May 4.

Diabetes Trials Unit, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.

Obesity increases the risk of developing cardiovascular disease and other metabolic diseases. We intended to compare three different anthropometric indicators of obesity, in predicting the incidence of cardiovascular events in Chinese type 2 diabetes. Beijing Community Diabetes Study was a prospective multi-center study conducted in Beijing community health centers. Type 2 diabetes patients from fourteen community health centers were enrolled at baseline. The primary endpoint was cardiovascular events. The upper quartile of neck circumference (NC) was set as greater NC. A total of 3299 diabetes patients were enrolled. In which, 941 (28.52%) had cardiovascular disease at baseline. Logistic analysis showed that central obesity (waist circumference (WC) above 90 cm in men and 85 cm in women) and greater NC were all related to baseline cardiovascular disease (adjusted OR = 1.49, and 1.55). After 10-year follow-up, 340 (10.31%) had cardiovascular events. Compared with patients without cardiovascular events, those having cardiovascular events had higher BMI, larger WC and NC. Cox regression analysis showed that greater WC and NC were all associated with the occurrence of cardiovascular events (adjusted HR = 1.41, and 1.38). A higher NC and WC might increase the risk of cardiovascular events by about 40% in type 2 diabetes patients in Beijing communities.
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http://dx.doi.org/10.1038/s41598-021-88927-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097072PMC
May 2021

Leaf morphology, photosynthesis and pigments change with age and sunlight in savin juniper.

Plant Biol (Stuttg) 2021 Mar 23. Epub 2021 Mar 23.

Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, Canada.

• Savin juniper is an excellent species for desertification control in arid and semi-arid areas, where it typically establishes under the protection of nurse plants. Ultimately, established plants emerge into full sunlight as they grow, and this transition is accompanied by an increase in the preponderance of scale-like vs. needle-like leaf forms. • To test how age and variable sunlight environments affect shade tolerance in savin juniper, we established a pot study under field conditions with two age cohorts (1- and 4-year-old rooted scions) and three sunlight regimes (10%, 50% and 100% light transmittance). We measured growth, leaf parameters, photosynthesis, chlorophyll fluorescence and foliar pigments on a monthly basis (seven growing months per year, from 2015 to 2017). • Overall, there was little interaction for all variables, and both cohort and light regime had significant effects. Leaf form and spacing varied continuously, tending towards shorter, more closely spaced and more appressed scale leaves with higher dry leaf mass per area in older plants or under higher sunlight. There were no clear age-related patterns in carotenoids but both cohort and light had significant effects on gas exchange and chlorophyll fluorescence variables. • Savin juniper shows an intermediate tolerance to shade that changes with growth in that younger plants were less tolerant of full sun than older plants, consistent with its reliance on nurse plants for ultimate establishment in the open.
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http://dx.doi.org/10.1111/plb.13256DOI Listing
March 2021

Chiral Analysis of Lactate during Direct Contact Coculture by Single-Cell On-Probe Enzymatic Dehydrogenation Derivatization: Unraveling Metabolic Changes Caused by d-Lactate.

Anal Chem 2021 03 3;93(10):4576-4583. Epub 2021 Mar 3.

State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

noncontact cell-based coculture models are frequently employed to study cell-to-cell communication. However, these models cannot accurately represent the complexity of signaling. d-Lactate is an unusual metabolite produced and released by cancer cells. The characterization of d-lactate is challenging as it shares the same mass but has much lower amounts compared with l-lactate. Herein, d-α-hydroxy acids were specifically recognized and dehydrogenated by d-α-hydroxy acid dehydrogenase. The dehydrogenation products were rapidly quaternized for enhancement of mass signals. An on-probe enzymatic dehydrogenation-derivatization method was proposed for chiral analysis of α-hydroxy acids at the single-cell level. It is a promising amplification methodology and affords over 3 orders of magnitude signal enhancement. Furthermore, direct contact coculture models were used to precisely mimic the tumor microenvironment and explore the communication between cancer and normal cells. Single-cell mass spectrometry (SCMS) was further applied to easily sample cell extracts and study the differences of the aspects of small molecule metabolism in cocultured cells. On the basis of direct contact coculture SCMS, several differential small molecule metabolites and differences of oxidative stress between cocultured and monocultured normal cells were successfully detected. Additionally, d-lactate was discovered as a valuable differential metabolite with application of the two developed methods. It may account for the cancer-associated metabolic behavior of normal cells. These changes could be relieved after d-lactate metabolism-related drug treatment. This discovery may promote the investigation of d-lactate metabolism, which may provide a novel direction for cancer therapy.
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http://dx.doi.org/10.1021/acs.analchem.0c05015DOI Listing
March 2021

The effects of acarbose therapy on reductions of myocardial infarction and all-cause death in T2DM during 10-year multifactorial interventions (The Beijing Community Diabetes Study 24).

Sci Rep 2021 Mar 1;11(1):4839. Epub 2021 Mar 1.

Majiapu Community Health Service Center, Beijing, China.

To investigate the potential benefits of acarbose therapy on cardiovascular events (CVD) in Type 2 diabetes (T2DM) in an urban community over 10-year follow-up. The study population of Beijing Community Diabetes Study (BCDS) were type 2 diabetes (T2DM) living in 21 communities in Beijing. All patients received comprehensive intervention in accordance with the Chinese guidelines for the prevention and treatment of diabetes. Professors in endocrinology from top tier hospitals regularly visited the communities for consultations, which was a feature of this study. A total of 1797 T2DM in BCDS study had complete screening data, including blood glucose, blood pressure, lipid profiles and acarbose continuous therapy. After 10-year follow-up, the risks of CVD outcomes were assessed according to whether patients had received acarbose therapy or not. All patients were followed-up to assess the long-term effects of the multifactorial interventions. At baseline, compared with the acarbose therapy free in T2DM, there was no significant difference in achieving the joint target control in patients with acarbose therapy. From the beginning of 8th year follow-up, the joint target control rate in patients with acarbose therapy was significantly higher than that of acarbose therapy free. During the 10-year follow-up, a total of 446 endpoint events occurred, including all-cause death, cardiovascular events, cerebrovascular events. The incidences of myocardial infarction (from the 4th year of follow-up) and all-cause death (from the 2nd year of follow-up) in patients who received acarbose therapy were significantly lower than that of acarbose therapy free respectively. In Cox multivariate analyses, there were significant differences in incidences of myocardial infarction and all-cause death between afore two groups during the 10-year follow-up, and the adjusted HRs were 0.50 and 0.52, respectively. After multifactorial interventions, T2DM with acarbose therapy revealed significant reductions of myocardial infarction and all-cause death. The long-term effects of with acarbose therapy on improving joint target control might be one of the main reasons of myocardial infarction and all-cause death reduction.Trial Registration: ChiCTR-TRC-13003978, ChiCTR-OOC-15006090.
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http://dx.doi.org/10.1038/s41598-021-84015-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921127PMC
March 2021

Long-term exposure to ozone and sulfur dioxide increases the incidence of type 2 diabetes mellitus among aged 30 to 50 adult population.

Environ Res 2021 03 5;194:110624. Epub 2021 Jan 5.

School of Public Health, College of Public Health, Taipei Medical University, No. 250 Wuxing St., Xinyi District, Taipei, 11031, Taiwan; Institute of Population Health Sciences, National Health Research Institutes, No.35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan; Master Program in Applied Epidemiology, College of Public Health, Taipei Medical University, No. 250 Wuxing St., Xinyi District, Taipei, 11031, Taiwan. Electronic address:

Aims/hypothesis: Worldwide, the information regarding the associations between long-term exposure to ozone (O) and sulfur dioxide (SO) and the development of type 2 diabetes remains scarce, especially in Asia. This study aimed to investigate the long-term effects of exposure to ambient O and SO on the incidence of type 2 diabetes with consideration of other air pollutants in Taiwanese adults aged 30 to 50 years.

Methods: A total of 6,426,802 non-diabetic participants aged between 30 and 50 years old were obtained from the National Health Insurance Research Database between 2005 and 2016. Incident type 2 diabetes was the main diagnosis at medical visits. Air quality data were provided by the Taiwan Environmental Protection Administration. The air pollutant concentrations for each participant were estimated using the ordinary kriging method to interpolate daily concentrations of O, SO, carbon monoxide (CO), nitrogen dioxide (NO), suspended fine particles (with an aerodynamic diameter less than 2.5 μm; PM), and suspended particles (with an aerodynamic diameter less than 10 μm; PM) in residential districts across Taiwan. Six-year average concentrations of pollutants were calculated from January 1, 2005 to December 31, 2010, and data were categorized into quartiles. We performed Cox regression models to analyze the long-term effects of exposure to O and SO on the incidence of type 2 diabetes.

Results: The hazard ratio (HR) for the incidence of diabetes per each interquartile range (IQR) increase in ozone exposure (3.30 ppb) was 1.058 (95% confidence interval (CI): 1.053, 1.064) and 1.011 (95% CI: 1.007, 1.015) for SO exposure (1.77 ppb) after adjusting for age, sex, socioeconomic status, urbanization level, temperature, humidity, and chronic comorbidities (Model 3). Furthermore, for every 3.30 ppb increase of O, the HR for incident type 2 diabetes was 1.093 (95% CI: 1.087, 1.100) after controlling factors shown in Model 3 plus SO and PM. On the other hand, for every 1.77 ppb increase of SO, the HR for incident type 2 diabetes was 1.073 (95% CI: 1.068, 1.079) after controlling factors shown in Model 3 plus NO and PM.

Conclusions: Long-term exposure to ambient O and SO was associated with a higher risk of developing type 2 diabetes for Taiwanese population. Exposure to O and SO may play a role in the adult early-onset type 2 diabetes.
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http://dx.doi.org/10.1016/j.envres.2020.110624DOI Listing
March 2021

, a new species of (Begoniaceae) from Guangdong, China.

PhytoKeys 2020 7;162:29-36. Epub 2020 Oct 7.

South China Limestone Plants Research Center, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China South China Agricultural University Guangzhou China.

A new species of Begonia section Coelocentrum, W.H. Tu, B.M. Wang & Y.L. Li from Guangdong Province, China, is described and illustrated here. Morphologically, the new species is most similar to T. C. Ku and Y. M. Shui & W. H. Chen, but differs from by its rugose leaves and glabrous capsules and from by its glabrous stipules without ciliate margin, densely hirsute-pilose leaves and obtuse apex of bracts. Additionally, it is also somewhat similar to Yan Liu, S. M. Ku & C.-I Peng, but there are significant distinctions in their stipules, leaves and bracts. The conservation status of is assessed as Critically Endangered (CR), according to the IUCN Red List Categories and Criteria.
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http://dx.doi.org/10.3897/phytokeys.162.51913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560510PMC
October 2020

Mass Spectrometry-Based Discovery of New Chemical Scaffold Rearrangement Ions: Aza-biphenylene as a Novel Potent Biradical Agent in Cancer Chemotherapy.

Anal Chem 2020 11 15;92(21):14517-14527. Epub 2020 Oct 15.

State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

Discovery of a new drug is time-consuming, laborious, and expensive. Herein, a novel integrative strategy for discovering potential new lead compounds has been developed, which was based on the characteristics of mass spectrometry (MS). MS was used to predict the potential forced degradation products (DPs) and metabolites of drugs by electrospray ionization and collision-induced dissociation (CID). Special rearrangement ions representing unique predicted DPs and metabolites were identified. The consistency between the predicted and the measured results was proven by metabolism and forced degradation of a commercial drug, respectively. From this, new chemical scaffold rearrangement ions named (aza)-biphenylenes, as potent anticancer agents, were discovered. As a representative aza-biphenylene analogue, 2-azabiphenylene was proven to induce apoptosis and inhibit the growth of various human cancer cells in a dose-dependent manner. Surprisingly, 2-azabiphenylene exhibited the best comparable bioactivity with the positive control sorafenib, but showed significantly lower cytotoxicity than sorafenib (at least a 5-fold decrease in cytotoxicity) because it could be targeted to the tumor microenvironment at low pH. A biradical mechanism accompanied by a mitochondrion-dependent oxidative stress mechanism was proposed to explore its anticancer mechanism. The highly reactive intermediate aza-biphenylenediyl worked as an active pharmaceutical ingredient and induced apoptosis of cancer cells. This provided the basis for the potential applications of CID-induced special rearrangement ions in developing new lead compounds.
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http://dx.doi.org/10.1021/acs.analchem.0c02669DOI Listing
November 2020

Taxonomic revision of the genus (Phyllanthaceae) in Taiwan, China.

PhytoKeys 2020 3;159:137-159. Epub 2020 Sep 3.

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 516650, China Zhongkai University of Agriculture and Engineering Guangzhou China.

A comprehensive taxonomic revision of the genus J.R. Forst. & G. Forst. from Taiwan in China was carried out based on the examination of herbarium specimens and filed investigations. Eight species and three varieties are recognized, including a new species endemic to Taiwan, Gang Yao & S.X. Luo, which is described and illustrated. Three names, viz. Hayata, Hayata, and F.Y. Lu & Y.S. Hsu, are reduced to the new synonyms of Blume, Hayata, and Wight, respectively. Two names, viz. Hayata and Hayata, are lectotypified here. A key to the species in Taiwan is provided.
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http://dx.doi.org/10.3897/phytokeys.159.54839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483396PMC
September 2020

Impact of meteorological factors on the occurrence of acute aortic dissection in Fujian Province, China: a single-center seven-year retrospective study.

J Cardiothorac Surg 2020 Jul 20;15(1):178. Epub 2020 Jul 20.

Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University, Fuzhou, China.

Background: The aim of this study was to investigate the correlation between meteorological factors and the occurrence of acute aortic dissection (AAD) in Fujian Province, China.

Methods: The clinical data of 2004 patients diagnosed with AAD in our hospital and the relevant local meteorological data from January 2013 to November 2019 were retrospectively analyzed.

Results: The incidence of AAD had a clear tendency toward concentration, and the corresponding peak in terms of the occurrence date was from January 13 to 14. The average minimum temperature, the average maximum temperature, and the average daily temperature differences on the "day with AAD" were significantly lower than those on the "day without AAD". From 5 days to 3 days before AAD onset, the average daily temperature difference showed a downward trend, but statistical analysis showed that the average minimum, average maximum and average daily temperature differences were not significantly different from the values 5 days to 0 days before AAD onset.

Conclusions: The incidence of AAD is related to the season and month. The lowest average temperature may increase the incidence of AAD in patients with complicated cardiovascular diseases.
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http://dx.doi.org/10.1186/s13019-020-01227-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372770PMC
July 2020

Comparative Study between Surgical Repair of Atrial Septal Defect via Median Sternotomy, Right Submammary Thoracotomy, and Right Vertical Infra-Axillary Thoracotomy.

Braz J Cardiovasc Surg 2020 06 1;35(3):285-290. Epub 2020 Jun 1.

Fujian Medical University Union Hospital Department of Cardiovascular Surgery Fujian People's Republic of China Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.

Objective: To compare the results of surgical repair via median sternotomy, right submammary thoracotomy, and right vertical infra-axillary thoracotomy for atrial septal defect (ASD).

Methods: This is a retrospective analysis of the relative perioperative and postoperative data of 136 patients who underwent surgical repair for ASD with the abovementioned three different treatments in our hospital from June 2014 to December 2017.

Results: The results of the surgeries were all satisfactory in the three groups. No statistically significant difference was found in operative time, duration of cardiopulmonary bypass, blood transfusion amount, postoperative mechanical ventilation time, duration of intensive care unit, length of hospital stay, and hospital costs. However, the median sternotomy group had the longest incision. Meanwhile, there was no significant difference in postoperative complications.

Conclusion: All three types of surgical incisions can be safely and effectively used to repair ASD. The treatments via right submammary thoracotomy and right vertical infra-axillary thoracotomy have advantages over the treatment via median sternotomy in cosmetic results and should be the recommended options.
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http://dx.doi.org/10.21470/1678-9741-2019-0096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299584PMC
June 2020

Liver segmentation from abdominal CT volumes based on level set and sparse shape composition.

Comput Methods Programs Biomed 2020 Oct 22;195:105533. Epub 2020 May 22.

School of Xiangya Hospital, Central South University, Changsha 410075, China. Electronic address:

Background And Objective: Liver segmentation from abdominal CT volumes is a primary step for computer-aided surgery and liver disease diagnosis. However, accurate liver segmentation remains a challenging task for intensity inhomogeneity and serious pathologies occurring in liver CT volume. This paper presents a novel framework for accurate liver segmentation from CT images.

Methods: Firstly, a novel level set integrated with intensity bias and position constraint is applied, and for normal liver, the generated liver regions are regarded as the final results. Then, for pathological liver, a sparse shape composition (SSC)-based method is presented to refine liver shapes, followed by an improved graph cut to further optimize segmentation results. The level set-based method is capable of overcoming intensity inhomogeneity in object regions, and the SSC- and graph cut-based strategy has outstanding power to address under-segmentation appearing in pathological livers.

Results: The experiments conducted on public databases SLIVER07 and 3Dircadb show that the proposed method can segment both healthy and pathological liver effectively. The segmentation performance in terms of mean ASD, RMSD, MSD, VOE and RVD on SLIVER07 are 0.9mm, 1.8mm, 19.4mm, 5.1% and 0.1%, respectively, and on 3Dircadb are 1.6mm, 3.1mm, 27.2mm, 9.2% and 0.5%, respectively, which outperforms many existing methods.

Conclusions: The proposed method does not require complex training procedure on numerous liver samples, and has satisfying and robust segmentation performance on both normal and pathological liver in various shapes.
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http://dx.doi.org/10.1016/j.cmpb.2020.105533DOI Listing
October 2020

Brucine N-Oxide Reduces Ethanol Intake and Preference in Alcohol-Preferring Male Fawn-Hooded Rats.

Alcohol Clin Exp Res 2020 06 17;44(6):1321-1328. Epub 2020 May 17.

Department of Molecular and Cellular Pharmacology, (J-hL), School of Pharmaceutical Sciences, Peking University, Beijing, China.

Background: Alcohol use disorder places a heavy burden on global public health systems and thus is in urgent need of improved pharmacotherapies. Previously, our group has demonstrated that 30 mg/kg of the indole alkaloid brucine significantly attenuates alcohol-drinking behavior; however, the high toxicity, poor water solubility, short half-life, and limited therapeutic window of brucine restrain its clinical application as an antialcoholism medication. We subsequently hypothesized that the oxide of brucine (brucine N-oxide) would produce a similar behavioral effect without the risk profile associated with brucine.

Methods: Male Fawn-Hooded rats with high innate alcohol preference underwent 2-bottle choice procedures (Experiments 1 to 3). Experiment 1 examined the effects of 7 daily BNO injections of 0, 30, 50, or 70 mg/kg (s.c.) on voluntary alcohol consumption (n = 9/group). Experiment 2 evaluated the impact of a single dose of 0 or 70 mg/kg BNO on the increased alcohol intake induced by a 4-day alcohol deprivation (n = 8/group). Experiment 3 tested the effect of 7 daily BNO injections of 0 or 70 mg/kg (s.c.) on sucrose preference (n = 6/group). Experiment 4 measured the median lethal dose (LD50) values of BNO and brucine to compare their acute toxicity in rats. Experiment 5 tested whether BNO (0, 30, 50, and 70 mg/kg, s.c.) affected locomotor activity using an open-field paradigm (n = 8/group). Finally, Experiment 6 evaluated the possible conditioned rewarding effects of 0, 30, 50, and 70 mg/kg BNO using the conditioned place preference paradigm (n = 6/group).

Results: BNO administration dose-dependently attenuated alcohol consumption without affecting food intake, total fluid consumption, or the natural preference for a sucrose solution, with 70 mg/kg BNO reducing consumption by 22.8%. A single dose of 70 mg/kg BNO significantly inhibited the alcohol deprivation effect. The LD50 values of BNO and brucine in rats were determined to be 1,103.5 ± 177.0 mg/kg and 264.6 ± 17.7 mg/kg, respectively. Finally, BNO administration did not affect spontaneous locomotor activity or induce a place preference.

Conclusions: BNO may help to control excessive alcohol use and should be considered a treatment strategy for future study and development.
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http://dx.doi.org/10.1111/acer.14344DOI Listing
June 2020

MicroRNA-153-3p enhances the sensitivity of chronic myeloid leukemia cells to imatinib by inhibiting B-cell lymphoma-2-mediated autophagy.

Hum Cell 2020 Jul 27;33(3):610-618. Epub 2020 Apr 27.

Department of Hematology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.

Chronic myeloid leukemia (CML) is a hematopoietic stem cell disease caused by abnormal DNA replication of bone marrow stem cells and chemotherapy resistance is a major obstacle to the effective treatment of patients with CML. Imatinib (IM), a tyrosine kinase inhibitor (TKI), is a first-line drug clinically used for CML. Mounting evidence has indicated that the dysregulation of microRNAs (miRNAs) is associated with the chemoresistance of CML. In this study, miR-153-3p, which had been implicated with numerous types of tumors, was identified to be downregulated in IM-resistant CML cells. Upregulation of miR-153-3p significantly increased IM sensitivity and decreased the survival rate of IM-resistant CML cells, whereas downregulation of miR-153-3p attenuated these effects in IM-resistant CML cells. Upregulated miR-153-3p could decrease the autophagy caused by IM in IM-resistant CML cells. Dual-luciferase reporter assays confirmed that Bcl-2 is a direct target of miR-153-3p. Bcl-2 restoration reversed the increased sensitivity to IM induced by miR-153-3p-mimic transfection in IM-resistant CML cells. The results of the present study showed that dysregulated miR-153-3p may target Bcl-2 to promote the development of IM resistance and attenuate IM-induced apoptosis in CML. Therefore, miR-153-3p upregulation combined with IM treatment may serve as a promising therapeutic strategy for patients with low sensitivity.
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http://dx.doi.org/10.1007/s13577-020-00367-1DOI Listing
July 2020

In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/yttria-stabilized zirconia as a novel bioactive material for bone screws: Biocompatibility and bioactivity.

J Biomater Appl 2020 07 4;35(1):108-122. Epub 2020 Apr 4.

Department of Orthopaedics, Affiliated Hospital of North Sichuan Medical College, Nanchong City, China.

Zirconia and its derivatives have been receiving increased levels of attention with regard to their potential application in bone tissue engineering. These materials are of particular interest because of their excellent characteristics, such as superior biological and mechanical properties. In this study, yttria-stabilized tetragonal zirconia (YTZ)-reinforced nanohydroxyapatite/polyamide 66 (nHA/PA66) bone screws were prepared. The biocompatibility and bioactivity of nHA/PA66/YTZ were evaluated in vitro using MC3T3-E1 cells. Biocompatibility and bioactivity experiments (cell counting kit-8 tests, cell immunofluorescence analysis, and polymerase chain reaction) showed that nHA/PA66/YTZ could facilitate the biological functions of MC3T3-E1 cells. The attachment, proliferation, spreading, and expression of genes associated with osteogenesis (collagen 1, osteopontin, and osteocalcin) in cells cultured with the nHA/PA66/YTZ composite were all superior compared with the control groups ( < 0.05). In addition, nHA/PA66/YTZ bone screws were implanted into the femoral condyles of rabbits, and titanium screws were employed as a control group; postoperative histology and blood analysis revealed no obvious damage to the liver, kidneys, or any other major organs in either of the experimental groups. Moreover, nHA/PA66/YTZ screws resulted in significantly better bone-implant contact interfaces and enhanced formation of trabecular bone ( < 0.05); these characteristics were markedly better than those in the group that received titanium screws. These observations indicate that YTZ-reinforced nHA/PA66 composites have significant potential for applications in bone tissue engineering.
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http://dx.doi.org/10.1177/0885328220916618DOI Listing
July 2020

Improved Framingham Risk Scores of Patients with Type 2 Diabetes Mellitus in the Beijing Community: A 10-Year Prospective Study of the Effects of Multifactorial Interventions on Cardiovascular Risk Factors (The Beijing Communities Diabetes Study 22).

Diabetes Ther 2020 Apr 21;11(4):885-903. Epub 2020 Feb 21.

Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Introduction: To date, research is lacking on the development of a cardiovascular disease (CVD) risk assessment tool for people with diabetes mellitus, in general, and for Chinese patients with diabetes in particular. We have explored CVD risk assessment tools for Chinese patients with diabetes. Here, we report our investigation of cardiovascular risk assessment using the improved Framingham Risk Score (I-FRS) in patients with type 2 diabetes mellitus (T2DM) in Beijing communities.

Methods: A total of 3232 patients with T2DM attending Beijing community health centers were enrolled in this study. FRS were used to predict CVD risk in all patients at baseline using the following risk scores for glycated hemoglobin (HbA1c) categories: 0 = HbA1c ≤ 7.0%; 1 = 7.0% < HbA1c  ≤ 7.9%;      2 = 8.0% < HbA1c ≤ 8.9%; and 3 = HbA1c > 9.0%. The I-FRS was use to stratify all patients into low (I-FRS < 10%), medium (I-FRS 10-20%), and high (I-FRS > 20%) FRS strata. All treatments administered in the Beijing Communities Diabetes Study were in accordance with national guidelines for T2DM in China, and patients regularly attended clinical consultations with professors in endocrinology, who were experts in their respective speciality, from top tier hospitals. After 10 years, patients were followed-up to assess the long-term effects of the multifactorial interventions. Statistical analysis was performed using SAS® software (SAS Institute, Inc., Cary, NC, USA).

Results: The receiver operating characteristic curve of the I-FRS showed significant prediction accuracy for the actual incidence of CVD events. At baseline, subjects in the high FRS stratum for diabetes were more prone to be elderly and to have a longer duration of T2DM, higher systolic blood pressure, and higher lipid profiles. Subjects in the medium and high FRS strata had a higher incidence of CVD events than those in the no-complications group (DM group with no blood pressure issues) (P < 0.001). The 10-year hazard ratios for CVD events in diabetic patients with I-FRS score > 20% was 12.5-fold higher than that of patients with I-FRS score < 10%. Multifactorial intervention significantly reduced the I-FRS of the three FRS strata in patients with T2DM. The post-intervention I-FRS for the hypertension and CVD groups of patients were significantly lower than the respective baseline I-FRS. Cox multivariate analyses revealed that patients in the medium and high FRS strata had higher incidences of endpoint events than those in the low FRS stratum.

Conclusions: The I-FRS plays an important role in predicting CVD in patients with T2DM. Multifactorial interventions for CVD risk factors over 10-year follow-up lowered the estimated 10-year risk for CVD events in persons with diabetes. We suggest the use of the I-FRS score to stratify a patient's risk of CVD when analyzing the efficacy of diabetes management. Aggressive risk reduction should be focused on those individuals with a high I-FRS score.

Trial Registration: ChiCTR-TRC-13003978 and ChiCTR-OOC-15006090.
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http://dx.doi.org/10.1007/s13300-020-00782-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136369PMC
April 2020

Further discussion with Li et al. (2013, 2019) regarding the "ITS pseudogene hypothesis" for Ophiocordyceps sinensis.

Mol Phylogenet Evol 2020 05 2;146:106728. Epub 2020 Jan 2.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, Qinghai, China; Shenzhen International Graduate School, Tsinghua University, Guangdong, China. Electronic address:

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http://dx.doi.org/10.1016/j.ympev.2019.106728DOI Listing
May 2020

Association between Neck Circumference and the Occurrence of Cardiovascular Events in Type 2 Diabetes: Beijing Community Diabetes Study 20 (BCDS-20).

Biomed Res Int 2019 11;2019:4242304. Epub 2019 Nov 11.

Bayer Healthcare Company Limited, Beijing, China.

Background: Neck circumference (NC) was found to be related to the risk factors of cardiovascular disease. However, the effects of NC on cardiovascular disease are still controversial. A prospective study of Chinese patients with type 2 diabetes was performed to evaluate the relationship between NC and cardiovascular disease.

Methods: A multicenter prospective study with eight-year follow-up was conducted in Beijing communities. Cardiovascular events were defined as myocardial infarction, unstable angina pectoris, hospitalization for heart failure, coronary revascularization, cardiac death, stroke, transient ischemic attack, and cerebral hemorrhage.

Results: A total of 3,009 diabetic patients were recruited. Following an eight-year follow-up, 211 patients with cardiovascular events (105 in men and 106 in women) were identified. All patients were categorized into two groups according to the upper quartile of NC (43 cm in men and 39 cm in women). The prevalence of cardiovascular events in men with an NC >43 cm (16.48%) was higher than that in the group with an NC <43 cm (8.16%, =0.007). The prevalence of cardiovascular events in women with the NC >39 cm (10.67%) was higher compared to the group with NC <39 cm (5.31%, =0.004). The longitudinal prevalence of cardiovascular events in groups with different NC increased with the increasing duration of follow-up ( < 0.001). Cox regression analysis showed that higher NC was associated with the occurrence of cardiovascular events after adjusting for confounding variables (adjusted HR = 2.305 (1.535-3.460)).

Conclusions: NC was associated with the occurrence of cardiovascular events in type 2 diabetes in Chinese communities, and greater NC may increase the risk of cardiovascular events by about 2.3-fold.
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http://dx.doi.org/10.1155/2019/4242304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925677PMC
May 2020

[Influence of genetic and environment factors on aggressive behavior in twin children].

Zhongguo Dang Dai Er Ke Za Zhi 2019 Dec;21(12):1212-1217

Institute of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China.

Objective: To investigate the influence of genetic and environmental factors on aggressive behavior in twin children, and to provide clues for further exploring the causal relationship between such factors and aggressive behavior.

Methods: A questionnaire was used to investigate aggressive behavior, temperament type and parenting style among 261 twin children aged 4-12.8 years. The Holzinger method and the maximum likelihood method were used to construct a structural equation model for the estimation of heritability. Binary logistic regression analysis, multivariate linear regression analysis and general linear model analysis were used to analyze the association between aggressive behavior and environmental factors in twin children.

Results: The heritability of aggressive behavior was 44.4% in twin children and the score of aggressive behavior showed moderate heritability (60.9% in boys and 65.6% in girls). The multivariate analysis showed that the score of aggressive behavior in twin children was affected by the mother's emotional warmth/understanding, the mother's punishment/severity and the father's overprotection (P<0.05). There was an interaction between the mother's emotional warmth/understanding and the father's overprotection (P<0.05).

Conclusions: Both genetic and environmental factors have influence on children's aggressive behavior, and parenting style is the main environmental factor affecting the aggressive behavior of twin children.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7389012PMC
December 2019

Cervical epidural hematoma after spinal manipulation therapy: a case report.

BMC Musculoskelet Disord 2019 Oct 22;20(1):461. Epub 2019 Oct 22.

Sichuan Provincial Orthopedic Hospital, Chengdu, 610041, Sichuan, China.

Background: Cervical spinal manipulation therapy is a common non-invasive treatment for neck pain and stiffness, and has been widely used in the population. However, most people do not pay attention to the potential risks of neck manipulation, such as ligament damage, fractures, and spinal cord injuries. Epidural hematoma is a disease in which blood accumulates in the epidural space of the vertebral body. This disease is usually caused by trauma or iatrogenic surgery, and may be associated with blood coagulopathies, neoplasms, or degenerative spinal disease. Reports of epidural hematoma caused by cervical spinal manipulation are rare.

Case Presentation: We present the case of a patient with tetraplegia and spinal shock after neck manipulation. A physical examination of the patient on admission found tenderness in the neck and increased muscle tension in both upper limbs. The superficial sensation of the upper limb disappeared, but the deep sensation still remained. The lower extremity had 0/5 power on both sides. The sensation below the T2 level completely disappeared. A cervical magnetic resonance imaging scan showed an acute posterior epidural hematoma from the C3-T3 vertebrae. Ultimately, the patient underwent emergency hematoma removal and showed partial improvement in symptoms of paralysis during follow-up.

Conclusions: Although spinal manipulation is simple and neck pain is common and recurrent in the general population, the basic condition and disease history of patients should be determined before manipulation. For high-risk patients, caution should be applied for cervical spinal manipulation or it should be prohibited. For a suspected hematoma, MRI should be used at an early stage to diagnose and locate the hematoma.
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http://dx.doi.org/10.1186/s12891-019-2871-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805498PMC
October 2019

MicroRNA-34a suppresses the invasion and migration of colorectal cancer cells by enhancing EGR1 and inhibiting vimentin.

Exp Ther Med 2019 Oct 30;18(4):2459-2466. Epub 2019 Jul 30.

Department of Pathology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.

MicroRNAs (miRNAs/miRs) are small non-coding RNAs that serve a post-transcriptional regulatory role in eukaryotes. Previous studies have demonstrated that the expression of miR-34a in colorectal cancer (CRC) tissues is decreased compared with that in normal colorectal tissues. However, the role of miR-34a in the invasion and metastasis of CRC remains unclear. In the present study, the levels of miR-34a expression were measured in various CRC cell lines. The cells were transfected with miR-34a mimics or inhibitors in order to assess the proliferation rate, and the colony forming, invasive and migratory abilities. Furthermore, the protein expression levels of vimentin and early growth response protein 1 (EGR1) were examined by western blot analysis. The results revealed that the expression of miR-34a was low in SW620, RKO, LoVo and Caco-2 cell lines and high in the SW480 and SW1116 cell lines. The migration, invasion and proliferation levels of SW480 cells were facilitated by decreasing the expression of miR-34a. Transient transfection with miR-34a mimics in SW620 cells caused a notable decrease in cell migration, invasion and proliferation levels compared with the control group, and a downregulation of vimentin and upregulation of EGR1 protein expression. The present study demonstrated that miR-34a was deregulated in a highly invasive CRC cell lines, and that it may attenuate the migratory, invasive and proliferative capabilities of CRC cells by enhancing the expression of EGR1 and inhibiting that of vimentin. The results of the present study represent important progress towards understanding the mechanisms of CRC recurrence and metastasis.
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http://dx.doi.org/10.3892/etm.2019.7826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755272PMC
October 2019

Minimally Invasive Video-assisted Mitral Valve Replacement with a Right Chest Small Incision in Patients Aged Over 65 Years.

Braz J Cardiovasc Surg 2019 08 27;34(4):428-435. Epub 2019 Aug 27.

Fujian Medical University Union Hospital Department of Cardiovascular Surgery Fuzhou Fujian People's Republic of China Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.

Objective: To analyze and summarize the clinical safety and feasibility of minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision in patients aged over 65 years.

Methods: The clinical data of 45 patients over 65 years old who had mitral valve disease were analyzed retrospectively from January 2014 to January 2017 at Union Hospital, Fujian Medical University. The patients were divided into two groups; 20 patients in group A, who underwent minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision, and 25 patients in group B, who underwent conventional mitral valve replacement. We collected and analyzed their relevant clinical data.

Results: The operation was completed successfully in both groups. Compared with group B, group A was clearly superior for postoperative analgesia time, postoperative hospital length of stay, thoracic drainage liquid, blood transfusion, and length of incision. There were no differences between the two groups in postoperative severe complications and mortality. More patients in group B had pulmonary infections and poor incision healing, while more patients in group A had postoperative pneumothorax and subcutaneous emphysema.

Conclusion: In patients aged over 65 years, minimally invasive video-assisted mitral valve replacement with a small incision in the right chest had the same clinical safety and efficacy as the conventional method.
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http://dx.doi.org/10.21470/1678-9741-2018-0409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713364PMC
August 2019

The Association between Metabolic Syndrome and Morbid Events in Type 2 Diabetes after a 7-Year Community Management: Beijing Community Diabetes Study 17.

J Diabetes Res 2019 12;2019:5237371. Epub 2019 Jun 12.

Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Background: To examine the association between morbid events and metabolic syndrome (MS) in patients with type 2 diabetes mellitus (T2DM).

Methods: A prospective, longitudinal, multicenter study was conducted at 13 community health centers associated with Beijing Tongren Hospital. From 2008 to 2015, there have been 3,525 T2DM patients being managed based on the Chinese guideline for T2DM. The morbid events included macrovascular events, diabetic kidney disease, ophthalmologic events, cancer, and all-cause death.

Results: At baseline, there were 2,708 people with MS and 817 without MS. After a seven-year management, there were 351 (12.96%) events in MS people and 74 (9.06%) events in people without MS ( = 0.003). The prevalence of macrovascular events (6.06%) was much higher in MS people than in people without MS (3.79%, = 0.013). Cox regression analysis showed an association between MS and morbid events even after adjusting for confounding variables (adjusted hazard ratio = 1.44). MS was also associated with macrovascular events (adjusted hazard ratio = 1.96). The occurrence of morbid events and macrovascular events was increased when the numbers of metabolic abnormalities were 1, 2, 3, and 4 ( < 0.001). There was no continuously statistically significant difference in the cumulative prevalence of morbid events between patients with MS and patients without MS during the first five years. However, after six or seven years, the cumulative prevalence of morbid events in patients with MS was continuously significantly higher than that in patients without MS (11.00% vs. 8.20%, 12.96% vs. 9.06%, < 0.05).

Conclusions: T2DM with MS had higher incidence of morbid events, especially cardiovascular events, even after integrated management. The occurrence of morbid and macrovascular events increased as the number of metabolic abnormalities increased. MS was associated with increased risk of morbid events by 44% and macrovascular events by 96%. It would take at least six years to observe the association between MS and morbid events in T2DM.
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http://dx.doi.org/10.1155/2019/5237371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594276PMC
February 2020

Twenty-four-hour ambulatory blood pressure changes in older patients with essential hypertension receiving monotherapy or dual combination antihypertensive drug therapy.

J Geriatr Cardiol 2019 Apr;16(4):354-361

Department of Traditional Chinese Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: To evaluate the differences in 24-hour ambulatory blood pressure (BP) in older patients with hypertension treated with the five major classes of antihypertensive drugs, as monotherapy or dual combination therapy, to improve daytime and nighttime BP control.

Methods: We enrolled 1920 Chinese community-dwelling outpatients aged ≥ 60 years and compared ambulatory BP values and ambulatory BP control (24-hour BP < 130/80 mmHg; daytime mean BP < 135/85 mmHg; and nighttime mean BP < 120/70 mmHg), as well as nighttime BP dip patterns for monotherapy and dual combination therapy groups.

Results: Patients' mean age was 71 years, and 59.5% of patients were women. Calcium channel blockers (CCBs) constituted the most common (60.3% of patients) monotherapy, and renin-angiotensin system (RAS) blockers combined with CCBs was the most common (56.5% of patients) dual combination therapy. Monotherapy with beta-blockers (BB) provided the best daytime BP control. The probabilities of having a nighttime dip pattern and nighttime BP control were higher in patients receiving diuretics compared with CCBs (OR = 0.52, = 0.05 and OR = 0.41, = 0.007, respectively). Patients receiving RAS/diuretic combination therapy had a higher probability of having controlled nighttime BP compared with those receiving RAS/CCB (OR = 0.45, = 0.004). Compared with RAS/diuretic therapy, BB/CCB therapy had a higher probability of achieving daytime BP control (OR = 1.27, = 0.45).

Conclusions: Antihypertensive monotherapy and dual combination drug therapy provided different ambulatory BP control and nighttime BP dip patterns. BB-based regimens provided lower daytime BP, whereas diuretic-based therapies provided lower nighttime BP, compared with other antihypertensive regimens.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2019.04.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503475PMC
April 2019

Author`s Reply.

Anatol J Cardiol 2019 03;21(3):178

Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University; Fuzhou-China.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457408PMC
March 2019

Author`s Reply.

Anatol J Cardiol 2019 03;21(3):177

Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University; Fuzhou-China.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457401PMC
March 2019

[A case report of primary aneurysmal bone cyst of patella].

Zhongguo Gu Shang 2019 Jan;32(1):82-84

Department of Orthopaedics, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan, China;

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http://dx.doi.org/10.3969/j.issn.1003-0034.2019.01.018DOI Listing
January 2019
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