Publications by authors named "Yu Zhang"

10,436 Publications

  • Page 1 of 1

Transcriptomic and metabolomic investigation of metabolic disruption in Vigna unguiculata L. triggered by acetamiprid and cyromazine.

Ecotoxicol Environ Saf 2022 May 23;239:113675. Epub 2022 May 23.

College of Food Science and Engineering, Hainan University, 570228 Haikou, China; Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, China; Laboratory of Quality and Safety Risk Assessment for Agro-products (Haikou), Ministry of Agriculture, China. Electronic address:

A variety of pesticides are often used in agricultural management to control target pests but may trigger disruptions in the metabolism of nontarget organisms, ultimately affecting crop quality. Acetamiprid (ACE) and cyromazine (CYR) are two frequently used insecticides on cowpea, so it is critical to understand whether these two insecticides cause metabolic disorders in cowpea quality changes and the mechanism by which they do so. Here, we used metabolomic and transcriptomic methods to explore the mechanisms of the effects of ACE, CYR, and their mixture (MIX) on cowpea. In this study, ACE, CYR and MIX had no significant effects on plant biomass or growth status but decreased the contents of starch, soluble protein, and total flavonoids. All treatments reduced the total flavonoid content, but MIX showed the largest reduction of 10.02%. Metabolomic and transcriptomic analyses revealed that ACE markedly affected amino acid metabolism, and CYR and MIX affected sugar metabolism and flavonoid synthesis pathways. ACE and CYR reduced the levels of alanine, glutamic acid, isoleucine and phenylalanine and the expression of amino acid-related genes in cowpea, while MIX significantly increased the levels of most amino acids. All pesticide treatments reduced saccharide levels and related genes, with the most pronounced reduction in the MIX treatment. Exposure to ACE decreased the content of naringenin chalcone and quercetin and increased the content of anthocyanins in cowpeas, while MIX caused a significant decrease in the contents of quercetin and anthocyanins. According to the current study, single and mixed pesticides had different effects on the active ingredients of cowpea, with MIX causing the most significant decrease in the metabolite content of cowpea. These results provide important insights from a molecular perspective on how neonicotinoids and triazine insecticides affect cowpea metabolism.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113675DOI Listing
May 2022

[Corrigendum] Sulforaphane suppresses the viability and metastasis, and promotes the apoptosis of bladder cancer cells by inhibiting the expression of FAT‑1.

Int J Mol Med 2022 Jul 26;50(1). Epub 2022 May 26.

Department of Urology, Baoan Central Hospital of Shenzhen, The Fifth Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518102, P.R. China.

Following the publication of the above paper, the authors drew the Editor's attention to the fact that their article contained two textual errors located in the same sentence: In the '' subsection of the Materials and methods section on p. 1086 (the first sentence), the text here should have read as follows (changes highlighted in bold): 'In the present study, 85 bladder cancer tissues and 27 adjacent normal tissues were collected from patients with BC who attended the for treatment.' (i.e. the samples were not exclusively collected from the Shenzhen Hospital of Southern Medical University as stated, and the collection dates should have been written as 2014 and 2018, not as 2010 and 2014). The authors are grateful to the Editor of for allowing them the opportunity to publish this Corrigendum, and they apologize for any inconvenience caused to the readership of the Journal. [International Journal of Molecular Medicine 46: 1085‑1095, 2020; DOI: 10.3892/ijmm.2020.4665].
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http://dx.doi.org/10.3892/ijmm.2022.5149DOI Listing
July 2022

Endomembrane-mediated storage protein trafficking in plants: Golgi-dependent or Golgi-independent?

FEBS Lett 2022 May 26. Epub 2022 May 26.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Seed storage proteins (SSPs) accumulated within plant seeds constitute the major protein nutrition sources for human and livestock. SSPs are synthesized on the endoplasmic reticulum (ER) and then deposited in plant-specific protein bodies (PBs), including ER-derived PBs and protein storage vacuoles (PSVs). Plant seeds have evolved a distinct endomembrane system to accomplish SSP transport. There are two distinct types of trafficking pathways contributing to SSP delivery to PSVs, one Golgi-dependent and the other Golgi-independent. In recent years, molecular, genetic and biochemical studies have shed light on the complex network controlling SSP trafficking, to which both evolutionarily conserved molecular machineries and plant-unique regulators contribute. In this review, we discuss current knowledge of PB biogenesis and endomembrane-mediated SSP transport, focusing on ER export and post-Golgi traffic. These knowledges support a dominant role for the Golgi-dependent pathways in SSP transport in Arabidopsis and rice. In addition, we describe cutting-edge strategies to dissect the endomembrane trafficking system in plant seeds to advance the field.
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http://dx.doi.org/10.1002/1873-3468.14374DOI Listing
May 2022

Study on the Economic Burden of Neurodevelopmental Diseases on Patients With Genetic Diagnosis.

Front Public Health 2022 9;10:887796. Epub 2022 May 9.

NHC Key Laboratory of Birth Defect for Research and Prevention (Maternal and Child Health Hospital of Hunan Province), Changsha, China.

Objective: To study the burden of neurodevelopmental diseases (NDDs) via cost-of-illness analysis of Chinese patients with genetic diagnosis.

Methods: We recruited NDD patients (0-18 years old) with genetic diagnosis (GD) from September 1, 2020 to January 30, 2021. We gathered basic information on the details of diagnosis, as well as the direct medical cost, direct non-healthcare cost and indirect cost before and after receiving GD. We corrected the cost for time biases by calculating the cost per day for each patient.

Results: For the 502 patients with NDDs, the mean age was 4.08 ± 3.47. The household income was 0.6 (0.4, 1.0) 10,000 CNY per-month on average. The direct medical cost, direct non-healthcare cost and indirect cost were 12.27 (7.36, 22.23) 10,000 CNY, 1.45 (0.73, 2.69)10,000 CNY and 14.14(4.80, 28.25) 10,000 CNY per patient, respectively. Every patient received 1.20 (0.34, 3.60) 10,000 CNY on average (15.91%) from insurance. The daily total cost after receiving GD were ~62.48% lower than those before GD (191.59 CNY vs. 71.45 CNY). The descend range of lab cost (95.77%, < 0.05) was the largest, followed by drugs (91.39%, < 0.05), hospitalization (90.85%, < 0.05), and consultation (57.41%, < 0.05). The cost of rehabilitation kept slightly increasing but there were no significant differences ( > 0.05). The daily direct medical cost of each patient fell by 75.26% ( < 0.05) from 311.79 CNY to 77.14 CNY when the diagnostic age was younger than 1, and declined by 49.30% ( < 0.05) and 8.97% ( > 0.05) when the diagnostic age was 1-3 and older than 3, respectively.

Conclusions: Early genetic diagnosis is crucial for to reducing the burden of disease because of the amount of money spent was lower when they are diagnosed at younger age. Patients with NDDs can incur a heavy economic burden, especially in rehabilitation cost and indirect cost, because the insurance coverage for patients is low, so it is urgent for governments to pay more attention to these issues.
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http://dx.doi.org/10.3389/fpubh.2022.887796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126321PMC
May 2022

Integrating Covalent Organic Framework with Transition Metal Phosphide for Noble-Metal-Free Visible-Light-Driven Photocatalytic H Evolution.

Small 2022 May 25:e2201340. Epub 2022 May 25.

School of Science, RMIT University, Melbourne, VIC, 3000, Australia.

2D covalent organic frameworks (COFs) are considered as one kind of the most promising crystalline porous materials for solar-driven hydrogen production. However, adding noble metal co-catalysts into the COFs-based photocatalytic system is always indispensable. Herein, through a simple solvothermal synthesis method, TpPa-1-COF, a typical 2D COF, which displays a wide light absorption region, is rationally combined with transition metal phosphides (TMPs) to fabricate three TMPs/TpPa-1-COF hybrid materials, named Ni P (Ni P or CoP)/TpPa-1-COF. The incorporated TMPs can be served as electron collectors for accelerating the transfer of charges on TpPa-1-COF, thus the composites are demonstrated to be efficient photocatalysts for promoting water splitting. Benefitting from the richer surface reactive sites and lower H* formation energy barrier, the Ni P can most effectively improve the photocatalytic performance of the TpPa-1-COF, and the H evolution rate can reach up to 31.6 µmol h , approximately 19 times greater than pristine TpPa-1-COF (1.65 µmol h ), and is comparable to the Pt/TpPa-1-COF (38.8 µmol h ). This work is the first example of combining COFs with TMPs to construct efficient photocatalysts, which may offer new insight for constructing noble-metal-free COF-based photocatalysts.
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http://dx.doi.org/10.1002/smll.202201340DOI Listing
May 2022

Anti-nuclear matrix protein 2+ juvenile dermatomyositis with severe skin ulcer and infection: A case report and literature review.

World J Clin Cases 2022 Apr;10(11):3579-3586

Department of Rheumatology and Immunology; Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation base of Child Development and Critical Disorders; Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Background: Juvenile dermatomyositis (JDM) is an idiopathic inflammatory myopathy that occurs in childhood. It is characterized by muscle weakness and a characteristic rash. Previous literature reports have rarely described JDM with severe skin ulcers and infections.

Case Summary: Herein, we describe a case of a 2-year-old female patient who suffered from JDM, whose myositis-specific autoantibodies were positive for anti-nuclear matrix protein 2 antibody, with progressively worsening skin ulcers and severe infections. The patient was treated with glucocorticoids and various immunosuppressants. Nevertheless, further progression of the disease and the combination of primary disease and severe infection in the later period were fatal.

Conclusion: In children, anti-nuclear matrix protein 2+ JDM combined with skin ulcers often indicates severe disease. In such cases, personalized treatment for the primary disease and infection prevention and control are essential.
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http://dx.doi.org/10.12998/wjcc.v10.i11.3579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048553PMC
April 2022

Targeting TROY-mediated P85a/AKT/TBX3 signaling attenuates tumor stemness and elevates treatment response in hepatocellular carcinoma.

J Exp Clin Cancer Res 2022 May 25;41(1):182. Epub 2022 May 25.

Department of Clinical Oncology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

Background: Previous in vitro hepatocyte differentiation model showed that TROY was specifically expressed in liver progenitor cells and a small proportion of hepatocellular carcinoma cells, suggesting that TROY may participate in hepatocellular carcinoma (HCC) stemness regulation. Here, we aim to investigate the role and mechanism of TROY in HCC pathogenesis.

Method: Bioinformatics analysis of the TCGA dataset has been used to identify the function and mechanism of TROY. Spheroid, apoptosis, and ALDH assay were performed to evaluate the stemness functions. Validation of the downstream pathway was based on Western blot, co-immunoprecipitation, and double immunofluorescence.

Results: HCC tissue microarray study found that a high frequency of TROY-positive cells was detected in 53/130 (40.8%) of HCC cases, which was significantly associated with poor prognosis and tumor metastasis. Functional studies revealed that TROY could promote self-renewal, drug resistance, tumorigenicity, and metastasis of HCC cells. Mechanism study found that TROY could interact with PI3K subunit p85α, inducing its polyubiquitylation and degradation. The degradation of p85α subsequently activate PI3K/AKT/TBX3 signaling and upregulated pluripotent genes expression including SOX2, NANOG, and OCT4, and promoted EMT in HCC cells. Interestingly, immune cell infiltration analysis found that upregulation of TROY in HCC tissues was induced by TGF-β1 secreted from CAFs. PI3K inhibitor wortmannin could effectively impair tumor stemness to sorafenib.

Conclusion: We demonstrated that TROY is an HCC CSC marker and plays an important role in HCC stemness regulation. Targeting TROY-positive CSCs with PI3K inhibitor wortmannin combined with chemo- or targeted drugs might be a novel therapeutic strategy for HCC patients.
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http://dx.doi.org/10.1186/s13046-022-02401-6DOI Listing
May 2022

BEXCIS: Bayesian methods for estimating the degree of the skewness of X chromosome inactivation.

BMC Bioinformatics 2022 May 24;23(1):193. Epub 2022 May 24.

Department of Biostatistics, State Key Laboratory of Organ Failure Research, Ministry of Education, and Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.

Background: X chromosome inactivation (XCI) is an epigenetic phenomenon that one of two X chromosomes in females is transcriptionally silenced during early embryonic development. Skewed XCI has been reported to be associated with some X-linked diseases. There have been several methods measuring the degree of the skewness of XCI. However, these methods may still have several limitations.

Results: We propose a Bayesian method to obtain the point estimate and the credible interval of the degree of XCI skewing by incorporating its prior information of being between 0 and 2. We consider a normal prior and a uniform prior for it (respectively denoted by BN and BU). We also propose a penalized point estimate based on the penalized Fieller's method and derive the corresponding confidence interval. Simulation results demonstrate that the BN and BU methods can solve the problems of extreme point estimates, noninformative intervals, empty sets and discontinuous intervals. The BN method generally outperforms other methods with the lowest mean squared error in the point estimation, and well controls the coverage probability with the smallest median and the least variation of the interval width in the interval estimation. We apply all the methods to the Graves' disease data and the Minnesota Center for Twin and Family Research data, and find that SNP rs3827440 in the Graves' disease data may undergo skewed XCI towards the allele C.

Conclusions: We recommend the BN method for measuring the degree of the skewness of XCI in practice. The R package BEXCIS is publicly available at https://github.com/Wen-YiYu/BEXCIS .
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http://dx.doi.org/10.1186/s12859-022-04721-yDOI Listing
May 2022

Carbon fiber coated by quinoa cellulose nanosheet with outstanding scaled salt self-cleaning performance and purification of organic and antibiotic contaminated water.

Sci Rep 2022 May 24;12(1):8777. Epub 2022 May 24.

Department of Chemistry, Xinzhou Teachers University, 1 Dun Qi Street, Xinzhou, 034000, Shan Xi, China.

To date, various solar driven evaporation technologies have been developed for treatment of seawater and wastewater but with the threat from salt polluted and single treatment of seawater. Herein, we develop a multifunctional evaporator constructed by carbon fiber coated by quinoa cellulose nanosheet (CFQC) with outstanding self-cleaning performance and good purification property for treatment of organic and antibiotic polluted water. The resulting Zn-CFQC exhibits good light to thermal performance which can absorb about 86.95% lights in the range of UV-Vis-NIR (200-2500 nm); therefore, the wet and dry surface temperatures of Zn-CFQC are held at 62.1 and 124.3 °C respectively, and keep a speed of 3.2 kg m h for water evaporating under 1000 W m illumination. Such good light-to-thermal capabilities can be mainly imputed to the unique surface microstructures of the carbon fiber which decorated by two-dimension cellulose and activated by ZnCl. Additionally, Zn-CFQC shows good salt automatic-cleaning capability at night and corresponding mechanism has been simply elucidated according to the chemical potential theory. The method of treatment of carbon fiber opens a new way for commercial carbon fiber utilization of solar assisted water purification.
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http://dx.doi.org/10.1038/s41598-022-12889-9DOI Listing
May 2022

Micropore-Forming Gelatin Methacryloyl (GelMA) Bioink Toolbox 2.0: Designable Tunability and Adaptability for 3D Bioprinting Applications.

Small 2022 May 23:e2106357. Epub 2022 May 23.

Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.

It is well-known that tissue engineering scaffolds that feature highly interconnected and size-adjustable micropores are oftentimes desired to promote cellular viability, motility, and functions. Unfortunately, the ability of precise control over the microporous structures within bioinks in a cytocompatible manner for applications in 3D bioprinting is generally lacking, until a method of micropore-forming bioink based on gelatin methacryloyl (GelMA) was reported recently. This bioink took advantage of the unique aqueous two-phase emulsion (ATPE) system, where poly(ethylene oxide) (PEO) droplets are utilized as the porogen. Considering the limitations associated with this very initial demonstration, this article has furthered the understanding of the micropore-forming GelMA bioinks by conducting a systematic investigation into the additional GelMA types (porcine and fish, different methacryloyl-modification degrees) and porogen types (PEO, poly(vinyl alcohol), and dextran), as well as the effects of the porogen concentrations and molecular weights on the properties of the GelMA-based ATPE bioink system. This article exemplifies not only the significantly wider range of micropore sizes achievable and better emulsion stability, but also the improved suitability for both extrusion and digital light processing bioprinting with favorable cellular responses.
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http://dx.doi.org/10.1002/smll.202106357DOI Listing
May 2022

The reduction of vitamin D in females with major depressive disorder is associated with worse cognition mediated by abnormal brain functional connectivity.

Prog Neuropsychopharmacol Biol Psychiatry 2022 May 20:110577. Epub 2022 May 20.

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; Research Center of Clinical Medical Imaging, Anhui Province, Hefei 230032, China; Anhui Provincial Institute of Translational Medicine, Hefei 230032, China. Electronic address:

Low vitamin D is linked to major depressive disorder (MDD) through affecting the brain. Gender difference is apparent in MDD and vitamin D level. We aimed to examine the association between gender, vitamin D, clinical presentations, and brain functional connectivity in a large cohort of MDD patients and comparison subjects. Resting-state functional MRI data from 122 patients and 119 controls were collected to perform a combined analysis of functional connectivity density (FCD) and seed-based functional connectivity (FC). Peripheral venous blood samples were obtained to measure serum concentration of vitamin D (SCVD). Clinical presentations (symptoms profiles and cognition) were also assessed. We found an interaction of group and gender on SCVD in which MDD patients demonstrated lower SCVD than controls in females rather than males. Concurrently, lower SCVD was associated with worse cognitive performance (prospective memory and sustained attention). Compared with controls, female MDD patients showed reduced FCD and FC of the left middle frontal gyrus, which were related to lower SCVD. Importantly, these FCD and FC changes mediated the relationship between lower SCVD and cognitive dysfunction. Our findings suggest that functional connectivity abnormalities may serve as neural substrates underlying the associations between low vitamin D and cognitive impairments in female MDD patients, providing unique insight into treatment and prevention of MDD and its related cognitive dysfunction from the perspective of regulating circulating vitamin D.
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http://dx.doi.org/10.1016/j.pnpbp.2022.110577DOI Listing
May 2022

Realizing Spontaneously Regular Stacking of Pristine Graphene Oxide by a Chemical-Structure-Engineering Strategy for Mechanically Strong Macroscopic Films.

ACS Nano 2022 May 23. Epub 2022 May 23.

State Key Laboratory of Organic-Inorganic Composites, College of Materials Science and Engineering, Beijing University of Chemical Technology, 100029 Beijing, China.

Mechanical-electrical properties of macroscopic graphene films derived from graphene oxide (GO) sheets are substantially restricted by their surface wrinkles and structural misalignment. Herein, we propose a chemical-structure-engineering strategy to realize the spontaneously regular stacking of modified GO (GO-) with trace carboxyl. The highly aligned GO- film delivers a fracture strength and modulus of nearly 3- and 5-fold higher than a wrinkled film with conventional Hummer's method derived GO (GO-). The favorable assembly pattern of GO- sheets is attributed to their decreased interfacial friction on the atomic scale, which weakens their local gelation capability for freer configuration adjustment during the assembly process. The chemical structure of GO- can be further engineered by an epoxide-to-hydroxyl reaction, achieving a record high tensile strength of up to 631 MPa for the pristine GO film. By exploring the relationship between the surface terminations of GO and its stacking mode, this work proves the feasibility to realize high-performance macroscopic materials with optimized microstructure through the chemical modulation of nanosheet assembly.
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http://dx.doi.org/10.1021/acsnano.1c10561DOI Listing
May 2022

The CXCL10/CXCR3 Axis Promotes Disease Pathogenesis in Mice upon CVA2 Infection.

Microbiol Spectr 2022 May 23:e0230721. Epub 2022 May 23.

Department of Epidemiology, College of Public Health, Zhengzhou Universitygrid.207374.5, Zhengzhou, China.

Coxsackievirus A2 (CVA2) is an emerging pathogen that results in hand-foot-and-mouth disease (HFMD) outbreaks. Systemic inflammatory response and central nervous system inflammation are the main pathological features of fatal HFMD. However, the immunopathogenesis of CVA2 infection is poorly understood. We first detected the transcriptional levels of 81 inflammation-related genes in neonatal mice with CVA2 infection. Remarkably, CVA2 induced higher expression of chemokine (C-X-C motif) ligand 10 (CXCL10) in multiple organs and tissues. CXCL10 acts through its cognate receptor chemokine (C-X-C motif) receptor 3 (CXCR3) and regulates immune responses. CXCL10/CXCR3 activation contributes to the pathogenesis of many inflammatory diseases. Next, we found CXCL10 and CXCR3 expression to be significantly elevated in the organs and tissues from CVA2-infected mice at 5 days postinfection (dpi) using immunohistochemistry (IHC). To further explore the role of CXCL10/CXCR3 in CVA2 pathogenesis, an anti-CXCR3 neutralizing antibody (αCXCR3) or IgG isotype control antibody was used to treat CVA2-infected mice on the same day as infection and every 24 h until 5 dpi. Our results showed that αCXCR3 therapy relieved the clinical manifestations and pathological damage and improved the survival rate of CVA2-infected mice. Additionally, αCXCR3 treatment reduced viral loads and reversed the proinflammatory cytokine (interleukin 6 [IL-6], tumor necrosis factor alpha [TNF-α], and IL-1β) expression, apoptosis, and inflammatory cell infiltration induced by CVA2. Collectively, our study presents evidence for the involvement of the CXCL10/CXCR3 axis in CVA2 pathogenesis. The activation of CXCL10/CXCR3 contributes to CVA2 pathogenesis by inducing apoptosis, proinflammatory cytokine expression, and inflammatory cell infiltration, which can be reversed by αCXCR3 therapy. This study provides new insight into the pathogenesis of HFMD, which has an important guiding significance for the treatment of HFMD. Systemic inflammatory response and central nervous system inflammation are the main pathological features of fatal HFMD cases. We detected the expression of 81 inflammation-related genes and found higher expression of CXCL10 in CVA2-infected mice. Next, we confirmed CXCL10/CXCR3 activation using immunohistochemistry and found that anti-CXCR3 neutralizing antibody (αCXCR3) therapy could relieve the clinical manifestations and pathological damage and improve the survival rate of CVA2-infected mice. Additionally, αCXCR3 treatment reduced viral loads and reversed the proinflammatory cytokine (IL-6, TNF-α, and IL-1β) expression, apoptosis, and inflammatory cell infiltration induced by CVA2. Collectively, our study presents the first evidence for the involvement of the CXCL10/CXCR3 axis in CVA2 pathogenesis. The activation of CXCL10/CXCR3 contributes to CVA2 pathogenesis via inducing apoptosis, proinflammatory cytokine expression, and inflammatory cell infiltration, which can be reversed by αCXCR3 therapy. This study provides new insight into the pathogenesis of HFMD, which has an important guiding significance for the treatment of HFMD.
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http://dx.doi.org/10.1128/spectrum.02307-21DOI Listing
May 2022

The effects of combined microwave ablation and open surgery for the treatment of lung cancer-derived thoracolumbar metastases.

Orthop Surg 2022 May 23. Epub 2022 May 23.

Department of Orthopaedic Oncology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objective: To investigate the clinical effects of microwave ablation (MWA) in addition to open surgery for the treatment of lung cancer-derived thoracolumbar metastases.

Methods: This was a single-institution, retrospective, cohort study. From January 2019 to December 2020, a total of 47 patients with lung cancer-derived thoracolumbar metastases underwent posterior spinal canal decompression and fixation surgery in our hospital. Two independent surgical teams treated these patients. One group underwent open surgery combined with MWA therapy, while the other had open surgery only (control). The pre- and post-operative visual analog scale (VAS) scores and the overall survival (OS) were compared between the MWA and control groups. The Frankel Grade classification was applied for the evaluation of the post-surgical spinal cord function. Improvement was defined as an increase of at least one rank from the pre-operative scores. Each patient was evaluated pre- and post-operatively at 48 h, 1 month, and 3-month intervals. Data on surgical-related complications were recorded.

Results: Thirty men and 17 women were included, with an average age of 57.9 ± 11.4 years (range, 26-81 years). Twenty-eight patients underwent MWA and were in the MWA group, and 19 patients were included in the control group. Post-operatively all patients were followed up regularly; the median follow-up time was 12 months (range, 3-24 months), and their median OS was 14 months. Patients in the MWA group had a lower VAS score than those in the control group at the 48-h (1.75 ± 1.01 vs 2.47 ± 0.96, P = 0.01) and 1-month (1.79 ± 0.92 vs 2.53 ± 1.35, P = 0.048) check-ups. At the 3-month evaluation, the VAS score differences between the two groups were not significant (P = 0.133). After surgery, spinal cord function improvement was not significantly different between the MWA and control groups (P = 0.515). MWA therapy combined with open surgery was not associated with increased OS compared with the control group (P = 0.492).

Conclusion: MWA can be an effective and safe pain-relief method but may not extend the OS of patients with lung cancer.
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http://dx.doi.org/10.1111/os.13236DOI Listing
May 2022

The potential, analysis and prospect of ctDNA sequencing in hepatocellular carcinoma.

PeerJ 2022 17;10:e13473. Epub 2022 May 17.

The Affiliated Nanhua Hospital of University of South China, Hengyang, Hunan, China.

Background: The genome map of hepatocellular carcinoma (HCC) is complex. In order to explore whether circulating tumor cell DNA (ctDNA) can be used as the basis for sequencing and use ctDNA to find tumor related biomarkers, we analyzed the mutant genes of ctDNA in patients with liver cancer by sequencing.

Methods: We used next-generation targeted sequencing technology to identify mutations in patients with liver cancer. The ctDNA from 10 patients with hepatocellular carcinoma (including eight cases of primary hepatocellular carcinoma and two cases of secondary hepatocellular carcinoma) was sequenced. We used SAMtools to detect and screen single nucleotide polymorphisms (SNPs) and insertion deletion mutations (INDELs) and ANNOVAR to annotate the structure and function of the detected mutations. Screening of pathogenic and possible pathogenic genes was performed using American College of Medical Genetics and Genomics (ACMG) guidelines. GO analysis and KEGG analysis of pathogenic and possible pathogenic genes were performed using the DAVID database, and protein-protein interaction network analysis of pathogenic and possible pathogenic genes was performed using the STRING database. Then, the Kaplan-Meier plotter database, GEPIA database and HPA database were used to analyse the relationship between pathogenic and possible pathogenic genes and patients with liver cancer.

Results: Targeted capture and deep sequencing of 560 cancer-related genes in 10 liver cancer ctDNA samples revealed 8,950 single nucleotide variation (SNV) mutations and 70 INDELS. The most commonly mutated gene was PDE4DIP, followed by SYNE1, KMT2C, PKHD1 and FN1. We compared these results to the COSMIC database and determined that ctDNA could be used for sequencing. According to the ACMG guidelines, we identified 54 pathogenic and possible pathogenic mutations in 39 genes in exons and splice regions of 10 HCC patients and performed GO analysis, KEGG analysis, and PPI network analysis. Through further analysis, four genes significantly related to the prognosis of liver cancer were identified.

Conclusion: In this study, our findings indicate that ctDNA can be used for sequencing. Our results provide some molecular data for the mapping of genetic variation in Chinese patients with liver cancer, which enriches the understanding of HCC pathogenesis and provides new ideas for the diagnosis and prognosis of HCC patients.
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http://dx.doi.org/10.7717/peerj.13473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121877PMC
May 2022

Network Pharmacology and Absolute Bacterial Quantification-Combined Approach to Explore the Mechanism of Tianqi Pingchan Granule Against 6-OHDA-Induced Parkinson's Disease in Rats.

Front Nutr 2022 6;9:836500. Epub 2022 May 6.

Department of Neurology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Parkinson's disease (PD) is the second most common neurodegenerative disease. Tianqi Pingchan Granule (TPG) is a clinically effective formula of traditional Chinese medicine to treat PD. However, the therapeutic effect and underlying mechanisms of TPG in PD remain unclear. Based on network pharmacology, the corresponding targets of TPG were identified using the Traditional Chinese Medicine Database and Analysis Platform Database. Differentially expressed genes in PD were obtained from the Therapeutic Target Database, Online Mendelian Inheritance in Man, GeneCards, and DrugBank databases. The protein-protein interaction (PPI) networks of intersected targets were constructed using the STRING database and visualized using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, and the pathways directly related to the pathogenesis of PD were integrated manually. Furthermore, studies were carried out based on network pharmacology. The gut microbiota, peripheral inflammatory cytokines, and glia-mediated neuroinflammation in substantia nigra were evaluated. A total of 99 target genes were intersected between targets of TPG and deferentially expressed genes in PD. The PPI network analysis indicated the proinflammatory cytokine as essential targets. GO and KEGG analyses indicated that inflammatory response and its related signaling pathways were closely associated with TPG-mediated PD treatment. studies revealed that class Negativicutes and order Selenomonadales decreased, whereas class Mollicutes, order Enterobacteriales, and Mycoplasmatales increased in fecal samples of PD rats 16S rRNA sequence analysis. Furthermore, the function prediction methods purposely revealed that TPG therapy may be involved in flavonoid biosynthesis, which have anti-inflammatory properties. In addition, studies revealed that TPG exposure was found to not only attenuate the production of peripheral inflammatory cytokines but also inhibit the activation of microglia and astrocytes in substantia nigra of PD rats. Through network pharmacology and experiment-combined approach, the mechanisms of TPG in the treatment of PD were revealed, and the role of TPG in the regulation of gut microbiota and inflammatory response was confirmed.
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http://dx.doi.org/10.3389/fnut.2022.836500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121100PMC
May 2022

Early Unplanned Reoperation After Glioma Craniotomy: Incidence, Predictor and Process Improvement.

Front Oncol 2022 6;12:898873. Epub 2022 May 6.

Department of Neurosurgery, Tangdu Hospital, Air Force Military Medical University, Xi'an, China.

Objective: To evaluate the rate of, reasons for, and predictors of unplanned reoperation after craniotomy for glioma in a single-institution consecutive series.

Methods: Patients who underwent glioma resection at our hospital from 2015 to 2021 were included (n=1563). Multivariate logistic regression was used to examine the predictors of early unplanned cranial reoperation. The predictors that were screened included patient age, sex, tumor properties, blood loss, blood pressure and antiplatelets drugs usage.

Results: A total of 3.6% (56/1563) of the patients underwent an early unplanned reoperation after craniotomy for glioma. The reasons for early unplanned reoperation were brain edema (48.2%), cerebral infarction (33.9%) and hemorrhage (17.9%). The predictors of early unplanned reoperation were WHO grade III-IV, peritumoral edema ≥1 cm, subtotal resection, arterial/venous involvement and elevation in blood pressure ≥50 mmHg.

Conclusions: Glioma properties and blood pressure management are decisive predictors of early unplanned reoperation for glioma resection. The authors provide a nuanced discussion regarding early unplanned reoperations and perioperative process improvement as a quality indicator for glioma patient populations.
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http://dx.doi.org/10.3389/fonc.2022.898873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121807PMC
May 2022

Biomimetic Hydrogel Scaffolds with Copper Peptide-Functionalized RADA16 Nanofiber Improve Wound Healing in Diabetes.

Macromol Biosci 2022 May 21:e2200019. Epub 2022 May 21.

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.

Wound healing in diabetes is retarded by the dysfunctional local microenvironment. Although there are many studies using hydrogels as substitutes for natural extracellular matrices (ECMs), hydrogels that can mimic both the structure and functions of natural ECM remain a challenge. Self-assembling peptide RADA16 nanofiber has distinct advantageous to provide a biomimetic extracellular matrix nanofiber structure. However, it is still lack of biological cues to promote angiogenesis that is of vital significance for diabetic wounds healing. With a customized copper peptide GHK functionalized RADA16, an integrated approach using functionalized RADA16 nanofiber to chelate copper ion, has been innovatively proposed in this present study. The acquired composite hydrogel held the biomimetic nanofiber architecture, and exhibited promoting angiogenesis by both enhancing adhesion and proliferation of endothelial cells (EC) in vitro and neovascularization in vivo. It showed that the functionalized nanofiber scaffolds significantly accelerated wound closure, collagen deposition, and tissue remodeling both in healthy and diabetic mice. Furthermore, immunohistochemical analysis gave evidence that an upregulated expression of eNOS and CD31 in the copper peptide-functionalized RADA16 treated group. It can be envisioned that this scaffold can serve as a promising dressing for diabetic wound healing. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mabi.202200019DOI Listing
May 2022

A novel solar-powered electrochemical mineralization system for persistent remediation of uranium-contaminated groundwater.

J Environ Radioact 2022 May 18;250:106909. Epub 2022 May 18.

School of Resource & Environment and Safety Engineering, University of South China, Hengyang, 421001, China. Electronic address:

Reduction of the migratory ability of uranium via reduction, co-precipitation or immobilization is a widely used technology for remediation of uranium contaminated groundwater (UCG). However, the re-released uranium due to environmental alterations such as oxidation, acid dissolution, or microbial decomposition limits the long-term effect of UCG remediation. Here, we developed a novel solar-powered electrochemical mineralization (SPEM) system for persistent remediation of UCG under laboratory conditions. The SPEM system incorporates uranium into magnetite crystal to achieve long-term stability of uranium. The effects of photoelectric conversion, subsurface void fraction, groundwater seepage velocity, and electrode configuration on uranium removal were systematically analyzed. The results showed that the remediation system had excellent adaptability to complex water quality and geological conditions, and could remediate large-area contamination. After 12 h of persistent treatment, the system with newly hexagonal two-dimensional electrode configuration (1A6C) reduced uranium concentration by more than 85% in simulated subsurface environment. The mineralized uranium was not re-released within continuous rinsing of treated regions using an acid solution (pH = 3.0), for 370 h. The developed method solely requires metallic iron as a raw material, which has high and long-term efficiency, is eco-friendly, simple, and widely applicable, thus reliable for the remediation of deep UCG.
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http://dx.doi.org/10.1016/j.jenvrad.2022.106909DOI Listing
May 2022

Knowledge, attitudes, and practices toward cancer pain management amongst healthcare workers (physicians, pharmacists, and nurses): a cross-sectional study from first-tier cities in China.

Support Care Cancer 2022 May 20. Epub 2022 May 20.

Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Purpose: China is currently one of the countries with the largest increased number of new cancer cases in the world, but cancer pain management (CPM) is still inadequate. This study uses a questionnaire to demonstrate the status and differences in knowledge, attitude and practice (KAP) of CPM among healthcare workers (HCWs) in developed regions of China, to find deficiencies and priorities for improvement, from which areas and advantages of the role of pharmacists and mobile devices can be explored.

Methods: This study used data from a questionnaire on CPM from March to June 2019. The study population consisted of a total of 515 HCWs in four first-tier developed cities in China. The questionnaire has four major components, analysis of differences in KAP of different occupations through one-way analysis of variance (ANOVA).

Results: Among the respondents, the physicians had the highest knowledge scores toward CPM, pharmacists had the lowest practice scores. Around half of the respondents indicated that their hospital or department have a pharmacist participating in CPM. Physicians and nurses were more likely to expect pharmacists to provide drug counseling. The HCWs interviewed most expect that the mobile-based pain management system can automatically screen and mark patients with pain.

Conclusion: From this study, it can be suggested that pharmacists and nurses in the CPM team should actively promote relevant knowledge. Besides, pharmacists should focus on improving practical ability such as increasing the frequency of pain assessment. Multidisciplinary collaboration and the introduction of mobile devices can improve and refine the CPM.
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http://dx.doi.org/10.1007/s00520-022-07139-7DOI Listing
May 2022

Au-FeO heterodimer multifunctional nanoparticles-based platform for ultrasensitive naked-eye detection of Salmonella typhimurium.

J Hazard Mater 2022 May 13;436:129140. Epub 2022 May 13.

College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, PR China. Electronic address:

In this work, we developed an ultrasensitive colorimetry for Salmonella typhimurium detection with multifunctional Au-FeO dumbbell-like nanoparticles (DBNPs) which possessed easy bio-modifiability, excellent LSPR characteristics, superparamagnetic properties and super peroxidase-like activity. In the detection, the anti-S. typhimurium antibody modified DBNPs (IDBNPs) bound with S. typhimurium and aggregated on their surfaces in a large number, which showed much quicker magnetic response than free IDBNPs. By controlling appropriate separation conditions, [email protected] typhimurium composites were captured, while free IDBNPs were remained in the supernatant. Therefore, by detecting the absorbance of the supernatant, quantitative detection was achieved from 10 to 1000 CFU/mL. Moreover, utilizing the peroxidase-like activity of IDBNPs, we further realized semi-quantitative naked-eye detection. By adding ABTS into the above supernatant, which was oxidized to green chelate (OxABTS), colorimetric signal was amplified significantly, and meanwhile, the green chelates and the wine-red IDBNPs engendered mixed color, enhancing the range of color gradation and greatly improving the visual resolution. Finally, a detection limit (10 CFU/mL) comparable with that of above spectrum measurement was achieved. Besides, our method exhibited efficient capture capability (nearly 100% even for rare S. typhimurium), and had good stability and specificity, and acceptable anti-interference ability in fetal bovine serum and milk samples.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129140DOI Listing
May 2022

Triclosan is the Predominant Antibacterial Compound in Ontario Sewage Sludge.

Environ Sci Technol 2022 May 20. Epub 2022 May 20.

Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.

Sewage treatment plants (STPs) accumulate both antibiotic and nonantibiotic antimicrobial compounds that can select for antibiotic resistant bacteria. Herein, we aimed to identify the predominant antibacterial compounds impacting from Ontario sewage sludge consisting of thousands of unknown compounds. Among the 10 extracted sludge samples, 6 extracts exerted significant growth inhibition effects in . A total of 103 compounds were tentatively detected across the 10 sludge samples by suspect screening, among which the bacterial enoyl-ACP reductase (FabI) inhibitor triclocarban was detected at the highest abundance. A hypomorphic FabI knockdown strain was highly susceptible to the sludge extracts, confirming FabI inhibitors as the primary antibacterial compounds in the sludge. Protein affinity pulldown identified triclosan as the major ligand binding to a His-tagged FabI protein from the sludge, despite the higher abundance of triclocarban in the same samples. Effect-directed analysis was used to determine the contributions of triclosan to the observed antibacterial potencies. Antibacterial effects were only detected in F and F across 20 fractions, which was consistent with the elution of triclosan and triclocarban in the same two fractions. Further, potency mass balance analysis confirmed that triclosan explained the majority (58-113%) of inhibition effects from sludge extracts. This study highlighted triclosan as the predominant antibacterial compound in sewage sludge impacting despite the co-occurrence of numerous other antibiotics and nonantibiotics.
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http://dx.doi.org/10.1021/acs.est.2c00406DOI Listing
May 2022

Privacy preserving mutli-source domain adaptaion for medical data.

IEEE J Biomed Health Inform 2022 May 20;PP. Epub 2022 May 20.

Great progress has been made in diagnosing medical diseases based on deep learning. Large-scale medical data are expected to improve deep learning performance further. It is almost impossible for a single institution to collect so much data due to the time-consuming and costly collection and labeling of medical data. Many studies have turned attention to data sharing among multiple medical institutions. However, due to different data acquiring and processing procedures, multiple institutions' medical data is characterized by distribution heterogeneity. Besides, the protection of patient privacy in medical data sharing has also been a common concern. To simultaneously address the problems of heterogeneous data distribution and privacy protection, we propose a novel multi-source source free domain adaptation. When aligning distributed heterogeneous data, our method only requires the pre-trained source models rather than direct access to the source domain data, thus protecting patients' privacy. In addition, it has the advantages of being efficient and less costly in network resources. The proposed method is evaluated on the multi-site fMRI database Autism Brain Imaging Data Exchange (ABIDE) and yields an average accuracy of 69.54%. We also analyzed its effectiveness on network resource-saving and conducted additional experiments on Camelyon17 to validate the generalization.
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http://dx.doi.org/10.1109/JBHI.2022.3175071DOI Listing
May 2022

CPT1C promotes the potential of gastric cancer ovarian metastasis through up-regulating fatty acid oxidation.

Acta Biochim Biophys Sin (Shanghai) 2022 Feb 25. Epub 2022 Feb 25.

Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

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http://dx.doi.org/10.3724/abbs.2022027DOI Listing
February 2022

Subsuming the Metal Seed to Transform Binary Metal Chalcogenide Nanocrystals into Multinary Compositions.

ACS Nano 2022 May 20. Epub 2022 May 20.

Department of Chemical Sciences and Bernal Institute, University of Limerick, V94T9PX Limerick, Ireland.

Direct colloidal synthesis of multinary metal chalcogenide nanocrystals typically develops dynamically from the binary metal chalcogenide nanocrystals with the subsequent incorporation of additional metal cations from solution during the growth process. Metal seeding of binary and multinary chalcogenides is also established, although the seed is solely a catalyst for nanocrystal nucleation and the metal from the seed has never been exploited as active alloying nuclei. Here we form colloidal Cu-Bi-Zn-S nanorods (NRs) from Bi-seeded CuS heterostructures. The evolution of these homogeneously alloyed NRs is driven by the dissolution of the Bi-rich seed and recrystallization of the Cu-rich stem into a transitional segment, followed by the incorporation of Zn to form the quaternary Cu-Bi-Zn-S composition. The present study also reveals that the variation of Zn concentration in the NRs modulates the aspect ratio and affects the nature of the majority charge carriers. The NRs exhibit promising thermoelectric properties with very low thermal conductivity values of 0.45 and 0.65 W/mK at 775 and 605 K, respectively, for Zn-poor and Zn-rich NRs. This study highlights the potential of metal seed alloying as a direct growth route to achieving homogeneously alloyed NRs compositions that are not possible by conventional direct methods or by postsynthetic transformations.
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http://dx.doi.org/10.1021/acsnano.1c11144DOI Listing
May 2022

Pigmented superficial basal cell carcinoma below the nipple.

Indian J Dermatol Venereol Leprol 2022 Feb 17. Epub 2022 Feb 17.

Department of Dermatology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China.

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http://dx.doi.org/10.25259/IJDVL_830_2021DOI Listing
February 2022

Brucella melitensis outer membrane protein 25 interacts with ferritin heavy polypeptide 1 in human trophoblast cells.

Mol Med Rep 2022 Jul 20;26(1). Epub 2022 May 20.

College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, P.R. China.

Outer membrane protein 25 (OMP25) is involved in virulence and serves an important role in pathogenesis during infection. However, the protein interacting with OMP25 in host cells remains to be elucidated. The present study constructed a cDNA library from 16M‑infected human placenta trophoblastic cells (HPT‑8) and identified and confirmed the interaction between OMP25 and ferritin heavy polypeptide 1 (FTH1) of HPT‑8 using yeast two‑hybrid and co‑immunoprecipitation technologies. The expression of Toll‑like receptor (TLR)4, myeloid differentiation primary response protein MyD88 (MyD88) mRNA and inflammatory factors was detected by RNA interference. The results showed that OMP25 interacted with FTH1. Subsequently, TLR4 and MyD88 mRNA expression levels and inflammatory factors, such as nitric oxide, lactate dehydrogenase and TNF‑α, significantly increased after inserting OMP25 into the HPT‑8 cells. Notably, a low dose of OMP25 resulted in immunological protection, whereas high dose of OMP25 resulted in a cytotoxic effect on the HPT‑8 cells. It is suggested that OMP25 and FTH1 serve important roles in intracellular parasitism of and inhibition of expression.
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http://dx.doi.org/10.3892/mmr.2022.12740DOI Listing
July 2022

Spin state engineering of spinel oxides by integration of Cr doping and a p-n junction for water oxidation.

Chem Commun (Camb) 2022 May 20. Epub 2022 May 20.

Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong 999077, China.

Spinel CoO has emerged as a promising electrocatalyst towards alkaline water oxidation, but its activity is restricted by the undesirable electronic configuration of the octahedral Co site. Herein, we simultaneously manipulate Cr doping and p-n junction engineering on CoO nanorods, which contributes to the formation of Co and Ni octahedral sites with optimal e fillings, thus achieving a superior OER performance.
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http://dx.doi.org/10.1039/d2cc02175gDOI Listing
May 2022

Mg(OH) nanosheets on Ti with immunomodulatory function for orthopedic applications.

Regen Biomater 2022 29;9:rbac027. Epub 2022 Apr 29.

Medical Research Center, Department of Orthopedics, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.

Macrophages play a vital role for guiding the fate of osteogenesis- related cells. It is well known that nano-topography and bioactive ions can directly enhance osteogenic behavior. However, the effects of nano-structure combined with bioactive ions release on macrophage polarization and the following osteogenesis and angiogenesis are rarely reported. Herein, Mg(OH) films with nano-sheet structures were constructed on the surface of Ti using hydrothermal treatment. The film presented nano-sheet topography and sustained release of Mg ions. The results of culture of bone marrow-derived macrophages (BMDMs), including PCR, western blot and flow cytometry suggested that the nano-Mg(OH) films were more favorable for macrophages polarizing to tissue healing M2 phenotype. Moreover, air-pouch model confirmed that the nano-Mg(OH) film coated Ti would induce milder inflammation and thinner fibrous layer , compared with untreated Ti. Furthermore, macrophages-conditioned culture mediums were collected from nano-Mg(OH) coated Ti group was superior for the osteogenic behaviors of mice bone marrow stem cells and the angiogenic behaviors of human umbilical vein endothelial cells. With harmonious early inflammatory response and subsequently improved osteogenesis and angiogenesis, the nano-Mg(OH) coated Ti is promising for orthopedic applications.
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http://dx.doi.org/10.1093/rb/rbac027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113411PMC
April 2022

Target receptor identification and subsequent treatment of resected brain tumors with encapsulated and engineered allogeneic stem cells.

Nat Commun 2022 May 19;13(1):2810. Epub 2022 May 19.

Department of Medicinal Chemistry, College of Pharmacy University of Utah, Salt Lake City, UT, 84112, USA.

Cellular therapies offer a promising therapeutic strategy for the highly malignant brain tumor, glioblastoma (GBM). However, their clinical translation is limited by the lack of effective target identification and stringent testing in pre-clinical models that replicate standard treatment in GBM patients. In this study, we show the detection of cell surface death receptor (DR) target on CD146-enriched circulating tumor cells (CTC) captured from the blood of mice bearing GBM and patients diagnosed with GBM. Next, we developed allogeneic "off-the-shelf" clinical-grade bifunctional mesenchymal stem cells (MSC) expressing DR-targeted ligand and a safety kill switch. We show that biodegradable hydrogel encapsulated MSC (EnMSC) has a profound therapeutic efficacy in mice bearing patient-derived invasive, primary and recurrent GBM tumors following surgical resection. Activation of the kill switch enhances the efficacy of MSC and results in their elimination post-tumor treatment which can be tracked by positron emission tomography (PET) imaging. This study establishes a foundation towards a clinical trial of EnMSC in primary and recurrent GBM patients.
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http://dx.doi.org/10.1038/s41467-022-30558-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120173PMC
May 2022
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