Publications by authors named "Yu Yin"

247 Publications

N-Myc promotes angiogenesis and therapeutic resistance of prostate cancer by TEM8.

Med Oncol 2021 Sep 14;38(10):127. Epub 2021 Sep 14.

Department of Pathology, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, People's Republic of China.

Although patients with early localized prostate cancer can survive longer, castration-resistant prostate cancer (CRPC) has gradually emerged with the use of androgen deprivation therapy (ADT). N-Myc and TEM8 play a vital role in the progression of several cancer types. However, the underlying mechanism of how N-Myc and TEM8 promote the progression of prostate cancer remains unclear. In this study, the expression of N-Myc and TEM8 was detected in benign prostatic hyperplasia (BPH) and prostate cancer (PCa) tissues by immunohistochemistry (IHC). LNCaP cell lines were maintained in RPMI 1640 medium supplemented with 10% charcoal-stripped fetal bovine serum. Subsequently, R language software was used to verify our results. Tubule formation assay of human umbilical vein endothelial cell (HUVEC) was conducted to examine the effect of N-Myc and TEM8 overexpression on angiogenesis in prostate cancer cells. IHC results showed a positive correlation between the expression of N-Myc and TEM8 in prostate cancer tissues. Further analysis showed that N-Myc and TEM8 were associated with clinicopathological features and poor prognosis in prostate cancer patients. Moreover, the overexpression of N-Myc and TEM8 promoted proliferation of prostate cancer cells and angiogenesis. Additionally, N-Myc and TEM8 overexpression was associated with therapeutic resistance. We further found that N-Myc promoted angiogenesis and therapeutic resistance in prostate cancer via TEM8. Hence, targeting N-Myc/TEM8 pathway in prostate cancer would be a novel therapeutic strategy to enhance the treatment of prostate cancer patients.
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http://dx.doi.org/10.1007/s12032-021-01575-xDOI Listing
September 2021

Comparison of Bioabsorbable and Metallic Interference Screws for Graft Fixation During ACL Reconstruction: A Meta-analysis of Randomized Controlled Trials.

Orthop J Sports Med 2021 Aug 18;9(8):23259671211021577. Epub 2021 Aug 18.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu City, Sichuan, China.

Background: Bioabsorbable interference screws and metallic interference screws are both widely used for graft fixation, but it remains unclear which screw type is superior.

Purpose: To compare clinical outcomes and complications between bioabsorbable and metallic interference screws for anterior cruciate ligament reconstruction (ACLR).

Study Design: Systematic review; Level of evidence, 1.

Methods: The literature was searched for relevant randomized controlled trials published between 1966 and 2020. Two investigators independently assessed risk of bias in the included studies, and data were pooled to calculate mean differences (MDs) for continuous outcomes and risk ratios (RRs) for dichotomous outcomes, together with 95% CIs. Meta-analysis was performed using a random- or fixed-effects model, depending on the heterogeneity in the data.

Results: Included were 14 randomized controlled trials involving 1032 patients who underwent ACLR: 528 patients with bioabsorbable screws and 504 patients with metallic screws. The 2 groups did not differ significantly in International Knee Documentation Committee score (RR, 1.04; 95% CI, 0.97 to 1.11), Lysholm score (MD, 0.59; 95% CI, -0.46 to 1.63), range of motion deficit (RR, 0.95; 95% CI, 0.67 to 1.34), positive pivot-shift test (RR, 0.87; 95% CI, 0.61 to 1.24), positive Lachman test (RR, 0.82; 95% CI, 0.48 to 1.39), or KT-1000 arthrometer value (MD, 0.01; 95% CI, -0.16 to 0.18). However, bioabsorbable screws were associated with a significantly higher risk of complications (RR, 1.70; 95% CI, 1.16 to 2.50), such as graft rupture, joint effusion, and infection.

Conclusion: The results of this review showed that there was no difference between metallic and bioabsorbable screws for ACLR in terms of subjective knee function or knee laxity, but metallic interference screws had fewer complications.
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http://dx.doi.org/10.1177/23259671211021577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377324PMC
August 2021

Annexin A8 regulated by lncRNA-TUG1/miR-140-3p axis promotes bladder cancer progression and metastasis.

Mol Ther Oncolytics 2021 Sep 20;22:36-51. Epub 2021 Apr 20.

Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, P.R. China.

Bladder cancer is the ninth most diagnosed cancer in the world. This study aims to investigate the role and mechanisms of the taurine-upregulated gene 1 (TUG1)/miR-140-3p/ () axis in bladder cancer. Western blotting and qRT-PCR determined the expression levels of , miR-140-3p, TUG1, and epithelial-mesenchymal transition (EMT) markers. RNA immunoprecipitation (RIP), luciferase assay, and RNA pull-down assay validated the association among ANXA8, miR-140-3p, and TUG1. The biological functions were determined by colony formation, Annexin V-fluorescein isothiocyanate (FITC)/propidium (PI) staining, and transwell assays. Xenograft tumorigenesis detected tumor growth and metastasis . Pathological analysis was examined by hematoxylin and eosin (H&E) and immunohistochemistry (IHC) analyses. ANXA8 was elevated in bladder tumors and cells. Knockdown of ANXA8 suppressed cell growth, migration, invasion, and EMT in UMUC-3 and T24 cells. was determined as a miR-140-3p target gene. Overexpression of miR-140-3p suppressed cell proliferation, migration, invasion, and EMT via targeting ANXA8. TUG1 promoted ANXA8 expression via sponging miR-140-3p. Silencing of miR-140-3p or ANXA8 overexpression abrogated the tumor-suppressive effects of TUG1 silencing on bladder cancer cell growth and metastasis. The TUG1/miR-140-3p/ANXA8 axis was also implicated in tumor growth and lung metastasis . TUG1 promotes bladder cancer progression and metastasis through activating ANXA8 by sponging miR-140-3p, which sheds light on the mechanisms of bladder cancer pathogenesis.
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http://dx.doi.org/10.1016/j.omto.2021.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8332373PMC
September 2021

Single-Centre Retrospective Training Cohort Using Artificial Intelligence for Prognostic Prediction of Encephalopathy, Mortality, and Liver Dysfunction after Early TIPS Creation.

Cardiovasc Intervent Radiol 2021 Jul 8. Epub 2021 Jul 8.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, No. 188, Shizi Street, Suzhou, 215006, China.

Objectives: Based on an artificial intelligence approach, this study attempted to establish prognostic models to predict 3-month overt hepatic encephalopathy (OHE) occurrence, 1-year mortality, and liver dysfunction for cirrhotic patients with acute variceal bleeding (AVB) treated with early transjugular intrahepatic portosystemic shunt (TIPS) creation.

Materials And Methods: This retrospective study included patients treated with early TIPS between January 2016 and November 2019. Independent risk factors associated with occurrence of OHE within 3 months, 1-year mortality, and liver dysfunction after early TIPS were identified using univariate and multivariate logistic analyses. Artificial neural network (ANN) models and prognostic nomograms based on the independent risk factors were established and validated internally.

Results: A total of 207 patients were included, with 33 (15.9%) experienced OHE within 3 months after TIPS creation. The albumin-bilirubin grade (P = 0.015), age (≤ 65, > 65 years) (P < 0.001), gender (P = 0.002), and alcoholic cirrhosis (P = 0.013) was identified as independent risk factors associated with 3-month OHE. Presence of portal vein thrombosis (P = 0.034) and model for end-stage liver disease score (P = 0.063) were identified as independent risk factors associated with 1-year mortality. The platelet-albumin-bilirubin grade (P = 0.041) and a history of hepatic encephalopathy (P = 0.018) were identified as independent risk factors associated with liver dysfunction after TIPS creation. Three ANN models and three nomograms were then established and validated with high accuracy.

Conclusions: The ANN and nomogram models have potential to accurately predict early occurrence of OHE, mortality, and liver dysfunction after early TIPS creation for cirrhotic patients with AVB.
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http://dx.doi.org/10.1007/s00270-021-02907-0DOI Listing
July 2021

Cloperastine inhibits esophageal squamous cell carcinoma proliferation in vivo and in vitro by suppressing mitochondrial oxidative phosphorylation.

Cell Death Discov 2021 Jun 21;7(1):166. Epub 2021 Jun 21.

The Pathophysiology Department, School of Basic Medical Sciences, College of Medicine, Zhengzhou University, Zhengzhou, 450000, China.

Esophageal squamous cell carcinoma (ESCC) is a major type of esophageal cancer. The prognosis of patients with ESCC remains poor because of the high morbidity and mortality of the disease. One strategy for drug discovery for ESCC treatment or prevention is screening FDA-approved drugs. In the present study, we found that the antitussive agent cloperastine can inhibit the proliferation of ESCC cells. However, the underlying mechanism was unclear. To determine the mechanism of this inhibitory effect, we performed proteomic analysis using KYSE150 cells treated with cloperastine and DMSO. The results identified several down-regulated signaling pathways included those of three key proteins (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex 1, NADH ubiquinone oxidoreductase subunit S5, and cytochrome C oxidase subunit 6B1) involved in oxidative phosphorylation. Meanwhile, we observed that oxidative phosphorylation in mitochondria was inhibited by the drug. Importantly, cloperastine suppressed ESCC growth in a xenograft mouse model in vivo. Our findings revealed that cloperastine inhibits the proliferation of ESCC in vivo and in vitro by suppressing mitochondrial oxidative phosphorylation.
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http://dx.doi.org/10.1038/s41420-021-00509-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257628PMC
June 2021

Automatic lumen segmentation using uniqueness of vascular connected region for intravascular optical coherence tomography.

J Biophotonics 2021 Jun 29:e202100124. Epub 2021 Jun 29.

School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, China.

We present an automatic lumen segmentation method using uniqueness of connected region for intravascular optical coherence tomography (IVOCT), which can effectively remove the effect on lumen segmentation caused by blood artifacts. Utilizing the uniqueness of vascular wall on A-lines, we detect the A-lines shared by multiple connected regions, identify connected regions generated by blood artifacts using traversal comparison of connected regions' location, shared ratio and area ratio and then remove all artifacts. We compare these three methods by 216 challenging images with severe blood artifacts selected from clinical 1076 IVOCT images. The metrics of the proposed method are evaluated including Dice index, Jaccard index and accuracy of 94.57%, 90.12%, 98.02%. Compared with automatic lumen segmentation based on the previous morphological feature method and widely used dynamic programming method, the metrics of the proposed method are significantly enhanced, especially in challenging images with severe blood artifacts.
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http://dx.doi.org/10.1002/jbio.202100124DOI Listing
June 2021

Angiomyolipoma of the left adrenal gland: a case report and literature review.

Gland Surg 2021 May;10(5):1826-1831

Department of Radiology, Medical Imaging Center of Guizhou Province, The Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Angiomyolipoma (AML) is apparent a part of mesenchymal tumor arising from perivascular epithelioid cell origin. AML is formed of blood vessels, spindle and epithelioid cells, and mature adipocytes. Adrenal AML is very rare. Indeed, only 21 cases of adrenal AML have been reported in the English-language literature. In these cases, patients were usually asymptomatic, and AML was often examined passingly during a health checkup or a common abdominal ultrasound (US) or a computed tomography (CT) examination for a other unrelated disease. This is a case report of adrenal AML in a 40-year-old male who presented with no clinical manifestations. Some 3 months ago, a first abdominal routine CT scan revealed a left adrenal mass. Three months later, a routine US showed a hypoechoic area of 21 mm × 15 mm in the left adrenal gland. A retroperitoneoscopic resection of the left adrenal gland tumor was then performed. A postoperative histopathology report confirmed a benign AML composed of blood vessels, smooth muscle, and mature adipose tissue. The patient was discharged without event. Due to the paucity of the literature on this subject, more clinical and imaging information about this disease is necessary for diagnosis, differential diagnosis, and treatment. In this paper, we present a complete case report, including the clinical presentation, imaging, treatment, and conduct a review of the relevant literature.
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http://dx.doi.org/10.21037/gs-21-244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184383PMC
May 2021

Integrated I-125 Seed Implantation Combined with Transarterial Chemoembolization for Treatment of Hepatocellular Carcinoma with Main Portal Vein Tumor Thrombus.

Cardiovasc Intervent Radiol 2021 Jun 11. Epub 2021 Jun 11.

Department of Interventional Radiology, First Affiliated Hospital, Soochow University, No. 188 Shizi Road, Suzhou, 215006, China.

Purpose: To compare the safety and efficacy of integrated iodine-125 (I-125) seed implantation (sequential implantation of helical I-125 seed implant into the main portal vein and of I-125 seeds into the branch tumor thrombus directly forming main portal vein tumor thrombus (MPVTT)) combined with transarterial chemoembolization (TACE) versus TACE alone for hepatocellular carcinoma (HCC) with MPVTT.

Materials And Methods: From December 2016 to January 2020, 46 HCC patients with MPVTT were analyzed. In the combination group, 21 patients received helical I-125 seed implantation in the main portal vein through a patent small portal vein branch and TACE in a single session. After 7-10 days, I-125 seeds were implanted percutaneously into the branch tumor thrombus directly forming MPVTT. In the TACE group, 25 patients received TACE alone. Thereafter, TACE was repeated as needed in both groups. Adverse events, tumor response, and overall survival (OS) of the two groups were compared.

Results: No adverse events grade ≥ 3 were observed in either group. The optimal objective response rate and disease control rate for MPVTT in the combination group and TACE group were 52.4% versus 4.0% (P < 0.001) and 85.7% versus 32.0% (P < 0.001), respectively. Median OS in the combination group (9.8 months) was longer than in the TACE group (5.2 months) (P = 0.024). Multivariate analysis revealed that, compared with the TACE group, the mortality risk in the combination group significantly decreased (hazard ratio: 0.444; P = 0.020).

Conclusion: Integrated I-125 seed implantation combined with TACE is a safe and effective treatment for HCC with MPVTT.

Level Of Evidence: Level 3, Non-randomized controlled cohort/follow-up study.
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http://dx.doi.org/10.1007/s00270-021-02887-1DOI Listing
June 2021

Single and joint inhibitory effect of nitrophenols on activated sludge.

J Environ Manage 2021 Sep 8;294:112945. Epub 2021 Jun 8.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing, 100012, China; College of Water Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address:

In this study, single and joint inhibitory effects of nitrophenols on activated sludge and variations on the content of extracellular polymeric substances (EPS) were investigated. Results indicate that the nitrophenols adversely affected the organic and NH-N removal of activated sludge and the adverse effect of nitrophenols on autotrophic bacteria was higher than that on heterotrophic bacteria. Further, 2,4-dinitrophenol (2,4-DNP) demonstrated the highest inhibitory effect, followed by 4-nitrophenol (4-NP) and 2-nitrophenol (2-NP), and the combined effects of binary and ternary nitrophenols induced additive toxicity. At various concentrations and toxicant ratios, 2,4-DNP, as the dominant toxic nitrophenol, was the major contributor to the joint inhibition effects of the mixed nitrophenols. At lower concentrations of 2-NP (below 100 mg/L), 4-NP (below 50 mg/L), and 2,4-DNP (below 10 mg/L), large amounts of both tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were secreted for the normal physiological activities of the microbiological cells. After further stimulation with higher concentrations of 2-NP (above 100 mg/L), 4-NP (above 50 mg/L), and 2,4-DNP (above 10 mg/L), the inhibitory effect of nitrophenols on bacterial metabolism evidently increased. However, the EPS production sharply reduced, particularly with respect to protein production. Parallel factor analysis for TB-EPS and LB-EPS further confirmed that the major proteins were tyrosine, tryptophan, and aromatic proteins. Thus, this study provides new insights into the inhibitory effects of mixed nitrophenols, which are frequently found in pharmaceutical and petrochemical effluents.
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http://dx.doi.org/10.1016/j.jenvman.2021.112945DOI Listing
September 2021

Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives.

Microb Cell Fact 2021 Jun 9;20(1):115. Epub 2021 Jun 9.

Department of Oncology of the First Affiliated Hospital, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.

Background: Interleukin-15 (IL-15) is a critical cytokine for the development, proliferation, and function of natural killer (NK) cells, NKT cells, and CD8 memory T cells and has become one of the most promising protein molecules for the treatment of cancer and viral diseases. However, there are several limitations in applying IL-15 in therapy, such as its low yield in vitro, limited potency, and short half-life in vivo. To date, there are several recombinant IL-15 agonists based on configurational modifications that are being pursued in the treatment of cancer, such as ALT-803, which are mainly produced from mammalian cells.

Results: In this study, we designed two different forms of the IL-15 complex, which were formed by the noncovalent assembly of IL-15 with dimeric or monomeric sushi domain of IL-15 receptor α (SuIL-15Rα)-IgG4 Fc fusion protein and designated IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc, respectively. The two IL-15 complexes were expressed in Pichia pastoris (P. pastoris), and their activities and half-lives were evaluated and compared. Pharmacokinetic analysis showed that IL-15/SuIL-15Rα-dFc had a half-life of 14.26 h while IL-15/SuIL-15Rα-mFc had a half-life of 9.16 h in mice, which were much longer than the 0.7-h half-life of commercial recombinant human IL-15 (rhIL-15). Treatment of mice with intravenous injection of the two IL-15 complexes resulted in significant increases in NK cells, NKT cells, and memory CD8 T cells, which were not observed after rhIL-15 treatment. Treatment of human peripheral blood mononuclear cells (PBMCs) from healthy donors with the two IL-15 complexes yielded enhanced NK and CD8 T cell activation and proliferation, which was comparable to the effect of rhIL-15.

Conclusions: These findings indicate that the IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc produced in P. pastoris exhibit potent activities and prolonged half-lives and may serve as superagonists for immunotherapy in further research and applications.
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http://dx.doi.org/10.1186/s12934-021-01605-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190845PMC
June 2021

Circ_0021087 acts as a miR-184 sponge and represses gastric cancer progression by adsorbing miR-184 and elevating FOSB expression.

Eur J Clin Invest 2021 Jun 2:e13605. Epub 2021 Jun 2.

Department of Obstetrics and Gynecology, Zhumadian Central Hospital Affiliated to Huanghuai University, Zhumadian City, China.

Background: Gastric cancer (GC) ranks third among the causes of cancer-related deaths in the world. Circular RNA hsa_circ_0021087 (circ_0021087) plays a repressive role in GC. Nevertheless, the mechanism by which circ_0021087 constrains GC advancement is unclear.

Materials And Methods: Expression patterns of circ_0021087, microRNA (miR)-184 and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) mRNA were assessed by quantitative real-time polymerase chain reaction (RT-qPCR). Gain-of-function experiments were conducted to verify the biological function of circ_0021087 in vitro and in vivo, including cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, transwell and xenograft assays. Protein levels were analysed by Western blotting and immunohistochemistry (IHC). The regulatory mechanism of circ_0021087 was analysed by bioinformatics analysis, dual-luciferase reporter and RNA immunoprecipitation (RIP) assays.

Results And Conclusion: Circ_0021087 and FOSB were lowly expressed in GC, whereas miR-184 had an opposite result. Circ_0021087 overexpression repressed GC cell proliferation and epithelial-mesenchymal transition (EMT) in xenograft models in vivo and induced GC cell apoptosis, repressed GC cell proliferation, EMT, migration and invasion in vitro. Circ_0021087 could elevate FOSB expression by adsorbing miR-184. MiR-184 mimic reversed the inhibitory influence of circ_0021087 overexpression on GC cell malignancy. Also, FOSB knockdown offset the suppressive impact of miR-184 silencing on GC cell malignancy. In conclusion, circ_0021087 played a repressive influence on GC progression by elevating FOSB expression by adsorbing miR-184, offering a new mechanism for circ_0021087 to inhibit the progression of GC.
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http://dx.doi.org/10.1111/eci.13605DOI Listing
June 2021

Structural Evolution of High-Performance Mn-Alloyed Thermoelectric Materials: A Case Study of SnTe.

Small 2021 Jun 25;17(25):e2100525. Epub 2021 May 25.

School of Mechanical and Mining Engineering, University of Queensland, Brisbane, Queensland, 4072, Australia.

Mn alloying in thermoelectrics is a long-standing strategy for enhancing their figure-of-merit through optimizing electronic transport properties by band convergence, valley perturbation, or spin-orbital coupling. By contrast, mechanisms by which Mn contributes to suppressing thermal transports, namely thermal conductivity, is still ambiguous. A few precedent studies indicate that Mn introduces a series of hierarchical defects from the nano- to meso-scale, leading to effective phonon scattering scoping a wide frequency spectrum. Due to insufficient insights at the atomic level, the theory remains as phenomenological and cannot be used to quantitatively predict the thermal conductivity of Mn-alloyed thermoelectrics. Herein, by choosing the SnTe as a case study, aberration-corrected transmission electron microscopy (TEM)/scanning transmission electron microscopy (STEM) to characterize the lattice complexity of Sn Mn Te is employed. Mn as a "dynamic" dopant that plays an important role in SnTe with respect to different alloying levels or post treatments is revealed. The results indicate that Mn precipitates at x = 0.08 prior to reaching solubility (≈10 mol%), and then splits into Mn substitution and γ-MnTe hetero-phases via mechanical alloying. Understanding such unique crystallography evolution, combined with a modified Debye-Callaway model, is critical in explaining the decreased thermal conductivity of Sn Mn Te with rational phonon scattering pathways, which should be applicable for other thermoelectric systems.
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http://dx.doi.org/10.1002/smll.202100525DOI Listing
June 2021

Efficiency of chitosan application against Phytophthora infestans and the activation of defence mechanisms in potato.

Int J Biol Macromol 2021 Jul 19;182:1670-1680. Epub 2021 May 19.

Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, PR China. Electronic address:

Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases that results in huge losses of potato crops worldwide. Chitosan as a defence elicitor can induce plant innate immunity against pathogen infection, but the efficiency and specific defence mechanism of chitosan against late blight in potato have not been elaborated. In this study, we demonstrated that the application of chitosan significantly enhanced potato resistance and reduced P. infestans infection in potted potato and in the field. Large-scale transcriptomic analysis suggested that chitosan preferentially activated several important pathways related to the plant defence response. Notably, we revealed that chitosan triggered pattern-triggered immunity responses in potato. Chitosan could trigger pattern recognition receptors to initiate intracellular signalling, and gradually amplify the immune signal. qRT-PCR verification showed that chitosan induced the expression of defence-related genes in potato. Moreover, treatment with chitosan result in Induced Systemic Resistance (ISR) in potato, including an accumulation of plant hormone salicylic acid, increase in the level of phenylalanine ammonia lyase activity and a content decrease of malondialdehyde. These findings help elucidate chitosan-mediated activation of the immune system in potato and provide a potential ecofriendly strategy to control potato late blight in the field.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.097DOI Listing
July 2021

A case report of atypical Kawasaki disease presented with severe elevated transaminases and literature review.

BMC Infect Dis 2021 May 4;21(1):415. Epub 2021 May 4.

Department of Paediatrics, ShaoXing KeQiao Women And Children's Hospital, ShaoXing, 312030, Zhejiang Province, PR China.

Background: Kawasaki disease (KD) is the most common cause of acquired heart disease among children in developed countries, in which the resulting coronary artery (CA) abnormalities cause myocardial ischemia, infarction, and death. Prompt diagnosis was essential, and supplemental information should be used to assist the diagnosis when classical clinical criteria are incomplete. The elevated levels of serum transaminases in most KD patients are mild. Herein, a case of atypical KD child with severely elevated transaminase was reported.

Case Presentation: A child with clinical manifestations of fever, high C-reactive protein (CRP) and severely elevated transaminases was reported. The treatment effect of antibiotic and liver-protecting drugs was not satisfactory. A bilateral diffuse dilation of the CA was detected on echocardiography on day 5 of the illness; thus, atypical KD was diagnosed. Elevated transaminases declined rapidly to normal after the treatment of intravenous immunoglobulin (IVIG). A 1-month follow-up revealed that CA returned to normal, and 2-month, 6-months, and 1-year follow-up revealed the child was in good general health.

Conclusions: This case highlighted that atypical KD clinical symptoms were diverse, and severely elevated transaminases might provide a clue to healthcare providers for the diagnosis and management of atypical KD.
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http://dx.doi.org/10.1186/s12879-021-06101-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094549PMC
May 2021

Comparison of Pharmacomechanical Catheter-Directed Thrombolysis versus Catheter-Directed Thrombolysis for the Treatment of Acute Iliofemoral Deep Vein Thrombosis: Measures of Long-Term Clinical Outcome and Quality of Life.

Ann Vasc Surg 2021 Apr 25. Epub 2021 Apr 25.

Department of Interventional Radiology, Nantong First People's Hospital, Nantong, Jiangsu, China. Electronic address:

Background: We studied the occurrence of post-thrombotic syndrome (PTS) in patients with either Pharmacomechanical Catheter-Directed Thrombolysis (hereafter "pharmacomechanical thrombolysis"; PT) or Catheter-Directed Thrombolysis (CDT) for the treatment of acute iliofemoral deep vein thrombosis (DVT).

Methods: This retrospective study of data archived between September 2013 and September 2015 was surveyed. Two separate patient populations were identified and analyzed: patients were separated into PT group or CDT group. For up to 5 years post-treatment, the incidence, severity of PTS, and chronic venous insufficiency questionnaire (CIVIQ) score difference were compared.

Results: The study identified 131 patients divided into PT group (65) and CDT group (66). Within the 5-year follow-up period, there was no significant difference in the incidence of PTS (45.0% PT vs. 57.6% CDT; odds ratio (OR) = 0.602; 95% confidence interval (CI), 0.291-1.242; P = 0.201), but there was reduced severe PTS in the PT group (Villalta scale ≥15 or ulcer:11.7% PT vs. 27.1% CDT; OR 0.355; 95%CI 0.134-0.941, P = 0.039; and Venous Clinical Severity Score (VCSS) ≥8: 13.3%PT vs. 28.8% CDT; OR 0.380; 95% CI 0.149-0.967, P = 0.045). There was also a larger improvement of venous disease-specific quality of life (QOL) in the PT group at 5 years [(62.89 ± 14.19) vs (56.39 ±15.62), P = 0.036] compared to the CDT group.

Conclusion: In patients with acute iliofemoral DVT treated with PT, PT significantly reduced PTS severity scores, and resulted in greater improvement in venous disease-specific QOL. However, the incidence of was not significantly different from that measured in the CDT.
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http://dx.doi.org/10.1016/j.avsg.2021.03.040DOI Listing
April 2021

The relationship between LncRNAs and lung adenocarcinoma as well as their ceRNA network.

Cancer Biomark 2021 ;31(2):165-176

China Medical University, Shenyang North New Area, Shenyang, Liaoning, China.

Background: More and more studies have shown that long non-coding RNA (LncRNA) as a competing endogenous RNA (ceRNA) plays an important role in lung cancer. Therefore, we analyzed the RNA expression profiles of 82 lung cancer patients which were all from Gene Expression Omnibus (GEO).

Methods: Firstly, we used BLASTN (evalue = 1e-10) to annotate the gene sets, performed in-group correction and batched normalization of the three data sets with R. Secondly, we used the limma and sva packages to compare tumor tissues with normal tissues. Then through WGCNA, we obtained the 4 gene modules most related to the trait.

Results: We intersected the genes of above 4 modules with the differential expression genes: 28 LncRNAs (up: 5, down: 23) and 265 mRNAs (up:11, down: 254). Based on these genes, we picked up 6 LncRNAs (CCDC39, FAM182A, SRGAP3-AS2, ADAMTS9-AS2, AC020907.2, SFTA1P), then set and visualized the LncRNA-miRNA-mRNA ceRNA network with 12 miRNAs related to 12 mRNAs. Finally, we performed downstream analysis of 265 mRNAs by Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and Protein-Protein Interaction (PPI) network.

Conclusion: After analyzing, we think this study provides a new direction for basic and clinical research related to LAD, and is expected to provide new targets for early diagnosis, prognostic evaluation and clinical treatment of lung cancer.
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http://dx.doi.org/10.3233/CBM-203078DOI Listing
January 2021

Connectome-based prediction of brain age in Rolandic epilepsy: a protocol for a multicenter cross-sectional study.

Ann Transl Med 2021 Mar;9(6):511

Department of Radiology, the Affiliated Hospital of Zunyi Medical University, Medical Imaging Center of Guizhou Province, Zunyi, China.

Background: Rolandic epilepsy (RE) is a common pediatric idiopathic partial epilepsy syndrome. Children with RE display varying degrees of cognitive impairment. In epilepsy, age-related neuroanatomic and cognitive changes differ greatly from those observed in the healthy brain, and may be defined as accelerated brain aging. Connectome-based predictive modeling (CPM) is a recently developed machine learning approach that uses whole-brain connectivity measured with neuroimaging data ("neural fingerprints") to predict brain-behavior relationships. The aim of the study will be to develop and validate a CPM for predicting brain age in patients with RE.

Methods: A multicenter, cross-sectional study will be conducted in 5 Chinese hospitals. A total of 100 RE patients (including 50 patients receiving anti-epileptic drugs and 50 drug-naïve patients) and 100 healthy children will be recruited to undergo a neuropsychological test using the Wechsler Intelligence Scale. Magnetic resonance images will also be collected. CPM will be applied to predict the brain age of children with RE based on brain functional connectivity.

Discussion: The findings of the study will facilitate our understanding of developmental changes in the brain in children with RE and could also be an important milestone in the journey toward developing effective early interventions for this disorder.

Trial Registration: The study has been registered with Chinese Clinical Trial Registry (ChiCTR2000032984).
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http://dx.doi.org/10.21037/atm-21-574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039653PMC
March 2021

GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein.

Nat Commun 2021 04 9;12(1):2114. Epub 2021 Apr 9.

State Key Laboratory of Proteomics, National Center of Biomedical Analysis, 27 Tai-Ping Road, Beijing, 100850, China.

Lack of detailed knowledge of SARS-CoV-2 infection has been hampering the development of treatments for coronavirus disease 2019 (COVID-19). Here, we report that RNA triggers the liquid-liquid phase separation (LLPS) of the SARS-CoV-2 nucleocapsid protein, N. By analyzing all 29 proteins of SARS-CoV-2, we find that only N is predicted as an LLPS protein. We further confirm the LLPS of N during SARS-CoV-2 infection. Among the 100,849 genome variants of SARS-CoV-2 in the GISAID database, we identify that ~37% (36,941) of the genomes contain a specific trio-nucleotide polymorphism (GGG-to-AAC) in the coding sequence of N, which leads to the amino acid substitutions, R203K/G204R. Interestingly, N exhibits a higher propensity to undergo LLPS and a greater effect on IFN inhibition. By screening the chemicals known to interfere with N-RNA binding in other viruses, we find that (-)-gallocatechin gallate (GCG), a polyphenol from green tea, disrupts the LLPS of N and inhibits SARS-CoV-2 replication. Thus, our study reveals that targeting N-RNA condensation with GCG could be a potential treatment for COVID-19.
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http://dx.doi.org/10.1038/s41467-021-22297-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035206PMC
April 2021

An efficient primary screening of COVID-19 by serum Raman spectroscopy.

J Raman Spectrosc 2021 Feb 19. Epub 2021 Feb 19.

Department of Radiation Oncology Sichuan Cancer Hospital & Institute Chengdu China.

The outbreak of COVID-19 coronavirus disease around the end of 2019 has become a pandemic. The preferred method for COVID-19 detection is the real-time polymerase chain reaction (RT-PCR)-based technique; however, it also has certain limitations, such as sample-dependent procedures with a relatively high false negative ratio. We propose a safe and efficient method for screening COVID-19 based on Raman spectroscopy. A total of 177 serum samples are collected from 63 confirmed COVID-19 patients, 59 suspected cases, and 55 healthy individuals as a control group. Raman spectroscopy is adopted to analyze these samples, and a machine learning support-vector machine (SVM) method is applied to the spectrum dataset to build a diagnostic algorithm. Furthermore, 20 independent individuals, including 5 asymptomatic COVID-19 patients and 5 symptomatic COVID-19 patients, 5 suspected patients, and 5 healthy patients, were sampled for external validation. In these three groups-confirmed COVID-19, suspected, and healthy individuals-the distribution of statistically significant points of difference showed highly consistency for intergroups after repeated sampling processes. The classification accuracy between the COVID-19 cases and the suspected cases is 0.87 (95% confidence interval [CI]: 0.85-0.88), and the accuracy between the COVID-19 and the healthy controls is 0.90 (95% CI: 0.89-0.91), while the accuracy between the suspected cases and the healthy control group is 0.68 (95% CI: 0.67-0.73). For the independent test dataset, we apply the obtained SVM model to the classification of the independent test dataset to have all the results correctly classified. Our model showed that the serum-level classification results were all correct for independent test dataset. Our results suggest that Raman spectroscopy could be a safe and efficient technique for COVID-19 screening.
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http://dx.doi.org/10.1002/jrs.6080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014023PMC
February 2021

Probiotic sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing .

Microorganisms 2021 Mar 31;9(4). Epub 2021 Mar 31.

College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.

contributes significantly to bacterial vaginosis, which causes an ecological imbalance in vaginal microbiota and presents with the depletion of sp. supplementation was reported to be an approach to treat bacterial vaginosis. We investigated the applicability of three sp. strains ( DM8909, ATCC14917, and ZX27) based on their probiotic abilities in vitro. The three candidate sp. strains for bacterial vaginosis therapy showed distinct properties in auto-aggregation ability, hydrophobicity, adhesion to cervical epithelial cells, and survivability in 0.01% hydrogen peroxide. ZX27 showed a higher yield in producing short-chain fatty acids and lactic acid among the three candidate strains, and all three sp. strains inhibited the growth and adhesion of . Furthermore, we discovered that the culture supernatant of sp. exhibited anti-biofilm activity against . In particular, the ZX27 supernatant treatment decreased the expression of genes related to virulence factors, adhesion, biofilm formation, metabolism, and antimicrobial resistance in biofilm-forming cells and suspended cells. Moreover, sp. decreased the upregulated expression of interleukin-8 in HeLa cells induced by or hydrogen peroxide. These results demonstrate the efficacy of sp. application for treating bacterial vaginosis by limiting the growth, adhesion, biofilm formation, and virulence properties of .
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http://dx.doi.org/10.3390/microorganisms9040728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065998PMC
March 2021

Energy-Efficient Ultrasonic Water Level Detection System with Dual-Target Monitoring.

Sensors (Basel) 2021 Mar 23;21(6). Epub 2021 Mar 23.

Department of Civil Engineering, The University of Texas at Arlington, Arlington, TX 76019, USA.

This study presents a developed ultrasonic water level detection (UWLD) system with an energy-efficient design and dual-target monitoring. The water level monitoring system with a non-contact sensor is one of the suitable methods since it is not directly exposed to water. In addition, a web-based monitoring system using a cloud computing platform is a well-known technique to provide real-time water level monitoring. However, the long-term stable operation of remotely communicating units is an issue for real-time water level monitoring. Therefore, this paper proposes a UWLD unit using a low-power consumption design for renewable energy harvesting (e.g., solar) by controlling the unit with dual microcontrollers (MCUs) to improve the energy efficiency of the system. In addition, dual targeting to the pavement and streamside is uniquely designed to monitor both the urban inundation and stream overflow. The real-time water level monitoring data obtained from the proposed UWLD system is analyzed with water level changing rate (WLCR) and water level index. The quantified WLCR and water level index with various sampling rates present a different sensitivity to heavy rain.
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http://dx.doi.org/10.3390/s21062241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061885PMC
March 2021

Oxoglaucine mediates Ca influx and activates autophagy to alleviate osteoarthritis through the TRPV5/calmodulin/CAMK-II pathway.

Br J Pharmacol 2021 08 5;178(15):2931-2947. Epub 2021 May 5.

Center for Materials Synthetic Biology, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Background And Purpose: Stimulation of calcium influx and suppression of autophagy play important roles in the pathogenesis of osteoarthritis (OA). In this study, we used a novel inhibitor of TRPV5 cation channels - oxoglaucine to attenuate progression of deterioration and pathological changes in OA patient-derived chondrocytes and OA animal model, by activating autophagy.

Experimental Approach: Inhibition by oxoglaucine of calcium influx was assessed in cells.. Analyses were also carried out to investigate the effect of oxoglaucine on OA by detection of anti-inflammatory response, TRPV5/CAMK-II/calmodulin pathway, autophagy, and cartilage protection both in vitro and in vivo. demonstrated by macroscopic evaluation and histological findings.

Key Results: Oxoglaucine suppressed expression of proinflammatory and apoptosis-related proteins, including TNF-α, IL-6, IL-1β, MMP-13, CASP-3, and BAX, and prevented matrix degradation in OA chondrocytes. It also successfully blocked Ca influx, activating autophagy dose-dependently asshown by up-regulated expression of LC-3II/I, Beclin-1, ATG5, ATG7, higher autophagic influx and formation of autophagic vesicles. It also decreased expression of mRNA and protein of TRPV5, CAMK-II, and calmodulin. Conversely, 1,25-dihydroxyvitamin D3, anagonist of TRPV5 channels, reversed the oxoglaucine-induced calcium influx inhibition and autophagy activation, demonstrating the association of oxoglaucine with TRPV5. Further, oxoglaucine prevented the apoptosis and matrix degradation of articular cartilage in a rat model of OA.

Conclusion And Implications: Oxoglaucine protects against cartilage damage by blocking the TRPV5/CAMK-II/calmodulin pathway to inhibit Ca influx and activate autophagy. Our results indicate that oxoglaucine has the potential to become a candidate drug for treatment of OA.
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http://dx.doi.org/10.1111/bph.15466DOI Listing
August 2021

Comparative analysis reveals the species-specific genetic determinants of ACE2 required for SARS-CoV-2 entry.

PLoS Pathog 2021 03 24;17(3):e1009392. Epub 2021 Mar 24.

Center for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, China.

Coronavirus interaction with its viral receptor is a primary genetic determinant of host range and tissue tropism. SARS-CoV-2 utilizes ACE2 as the receptor to enter host cell in a species-specific manner. We and others have previously shown that ACE2 orthologs from New World monkey, koala and mouse cannot interact with SARS-CoV-2 to mediate viral entry, and this defect can be restored by humanization of the restrictive residues in New World monkey ACE2. To better understand the genetic determinants behind the ability of ACE2 orthologs to support viral entry, we compared koala and mouse ACE2 sequences with that of human and identified the key residues in koala and mouse ACE2 that restrict viral receptor activity. Humanization of these critical residues rendered both koala and mouse ACE2 capable of binding the spike protein and facilitating viral entry. Our study shed more lights into the genetic determinants of ACE2 as the functional receptor of SARS-CoV-2, which facilitates our understanding of viral entry.
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http://dx.doi.org/10.1371/journal.ppat.1009392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990223PMC
March 2021

A glutaminase isoform switch drives therapeutic resistance and disease progression of prostate cancer.

Proc Natl Acad Sci U S A 2021 Mar;118(13)

Department of Pathology, Duke University School of Medicine, Durham, NC 27710;

Cellular metabolism in cancer is significantly altered to support the uncontrolled tumor growth. How metabolic alterations contribute to hormonal therapy resistance and disease progression in prostate cancer (PCa) remains poorly understood. Here we report a glutaminase isoform switch mechanism that mediates the initial therapeutic effect but eventual failure of hormonal therapy of PCa. Androgen deprivation therapy inhibits the expression of kidney-type glutaminase (KGA), a splicing isoform of glutaminase 1 (GLS1) up-regulated by androgen receptor (AR), to achieve therapeutic effect by suppressing glutaminolysis. Eventually the tumor cells switch to the expression of glutaminase C (GAC), an androgen-independent GLS1 isoform with more potent enzymatic activity, under the androgen-deprived condition. This switch leads to increased glutamine utilization, hyperproliferation, and aggressive behavior of tumor cells. Pharmacological inhibition or RNA interference of GAC shows better treatment effect for castration-resistant PCa than for hormone-sensitive PCa in vitro and in vivo. In summary, we have identified a metabolic function of AR action in PCa and discovered that the GLS1 isoform switch is one of the key mechanisms in therapeutic resistance and disease progression.
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http://dx.doi.org/10.1073/pnas.2012748118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020804PMC
March 2021

l-Arginine Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Ovine Intestinal Epithelial Cells by Regulating Apoptosis, Mitochondrial Function, and Autophagy.

J Nutr 2021 04;151(4):1038-1046

Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Background: Previous studies demonstrated that dietary l-arginine (Arg) alters the equilibrium between reactive oxygen species (ROS) generation and biological defenses to resist oxidant-induced toxicity. Whether supplying Arg can protect ovine intestinal epithelial cells (OIECs) from hydrogen peroxide (H2O2)-induced oxidative damage is unclear.

Objectives: The current study aimed to examine the effect of Arg on mitophagy, mitochondrial dysfunction, and apoptosis induced by H2O2 in OIECs.

Methods: The OIECs were incubated in Arg-free DMEM supplemented with 100 μM Arg (CON) or 350 μM Arg (ARG) alone or with 150 μM H2O2 (CON + H2O2, ARG + H2O2) for 24 h. Cellular apoptosis, mitochondrial function, autophagy, and the related categories of genes and proteins were determined. All data were analyzed by ANOVA using the general linear model procedures of SAS (SAS Institute) for a 2 × 2 factorial design.

Results: Relative to the CON and ARG groups, H2O2 administration resulted in 44.9% and 26.5% lower (P < 0.05) cell viability but 34.7% and 61.8% greater (P < 0.05) ROS concentration in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 40.7% and 28.8% lower (P < 0.05) ROS concentration but 14.9%-49.0% and 29.3%-64.1% greater (P < 0.05) mitochondrial membrane potential, relative mitochondrial DNA content, and complex (I-IV) activity in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 33.9%-53.1% and 22.4%-49.1% lower (P < 0.05) mRNA abundance of proapoptotic genes, respectively. Relative to the CON and CON + H2O2 groups, Arg supplementation resulted in 33.0%-59.2% and 14.6%-37.7% lower (P < 0.05) abundance of proapoptotic, mitophagy, and cytoplasmic cytochrome c protein, respectively.

Conclusions: Supply of Arg protects OIECs against H2O2-induced damage partly by improving mitochondrial function and alleviating cellular apoptosis and autophagy.
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http://dx.doi.org/10.1093/jn/nxaa428DOI Listing
April 2021

Anode biofilm influence on the toxic response of microbial fuel cells under different operating conditions.

Sci Total Environ 2021 Jun 2;775:145048. Epub 2021 Feb 2.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China. Electronic address:

The response of microorganisms in microbial fuel cells (MFCs) to toxic compounds under different operating conditions, such as flow rate and culture time, was investigated herein. While it has been reported that MFCs can detect some toxic substances, it is unclear if operating conditions affect MFCs toxicity response. In this study, the toxic response time of MFCs decreased when the flow rate increased from 0.5 mL/min to 2 mL/min and then increased with 5 mL/min. The inhibition rates at 0.5 mL/min, 2 mL/min, and 5 mL/min were 8.4% ± 1.6%, 45.1% ± 5.3%, and 4.9% ± 0.3%, respectively. With the increase of culture time from 7 days to 90 days, the toxic response time of MFCs gradually increased. The inhibition rates at culture times of 7 days, 45 days, and 90 days were 45.1% ± 5.3%, 32.6% ± 6.6%, and 23.2% ± 1.3%, respectively. Increasing the culture time will reduce the sensitivity of MFC. The results showed that MFCs can respond quickly at a flow rate of 2 mL/min after cultivation for 7 days. Under these conditions, the power density can reach 1137.0 ± 65.5 mW/m, the relative content of Geobacter sp. is 57%, and the ORP of the multilayers changed from -159.2 ± 1.6 mV to -269.9 ± 1.7 mV within 200 μm biofilm thickness. These findings show that increasing the flow rate and shortening the culture time are conducive for the toxicity response of MFCs, which will increase the sensitivity of MFCs in practical applications.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145048DOI Listing
June 2021

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Nat Commun 2021 02 11;12(1):961. Epub 2021 Feb 11.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China.

The global spread of SARS-CoV-2 is posing major public health challenges. One feature of SARS-CoV-2 spike protein is the insertion of multi-basic residues at the S1/S2 subunit cleavage site. Here, we find that the virus with intact spike (Sfull) preferentially enters cells via fusion at the plasma membrane, whereas a clone (Sdel) with deletion disrupting the multi-basic S1/S2 site utilizes an endosomal entry pathway. Using Sdel as model, we perform a genome-wide CRISPR screen and identify several endosomal entry-specific regulators. Experimental validation of hits from the CRISPR screen shows that host factors regulating the surface expression of angiotensin-converting enzyme 2 (ACE2) affect entry of Sfull virus. Animal-to-animal transmission with the Sdel virus is reduced compared to Sfull in the hamster model. These findings highlight the critical role of the S1/S2 boundary of SARS-CoV-2 spike protein in modulating virus entry and transmission and provide insights into entry of coronaviruses.
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http://dx.doi.org/10.1038/s41467-021-21213-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878750PMC
February 2021

Pyridazine-bridged expanded rosarin and semi-rosarinogen.

Chem Commun (Camb) 2021 Feb;57(12):1486-1489

Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science, South-Central University for Nationality, Wuhan, Hubei 430074, China.

The synthesis of the pyridazine-bridged expanded rosarin 1 and a reduced precursor, semi-rosarinogen 2, is reported. A single crystal X-ray diffraction analysis of 1 and theoretical calculations show that both 1 and 2 have distorted structures. Expanded rosarin 1 and its precursor 2 can differentiate various thiols in organic solvents by means of species-specific colour changes and reaction times.
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http://dx.doi.org/10.1039/d0cc07433kDOI Listing
February 2021

Seven-year disease-free survival in a patient with osteoclast-like giant cell-containing pancreatic undifferentiated carcinoma: a case report and literature review.

Int J Clin Exp Pathol 2020 1;13(12):3200-3205. Epub 2020 Dec 1.

Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida Gainesville, FL, USA.

Background: Undifferentiated carcinoma with osteoclast-like giant cells (UCOGC) of the pancreas is a very rare variant of pancreatic malignant neoplasm. It is regarded as a highly aggressive tumor with a worse prognosis than conventional pancreatic ductal adenocarcinoma.

Case Presentation: A 54-year-old male patient presented with 3-month recurrent epigastric distress. Computed tomography of the abdomen showed a large cystic mass in the distal pancreas. On macroscopic examination, the lesion had numerous multiloculated cystic cavities. Microscopically, the tumor predominantly comprised a considerable number of evenly distributed non-neoplastic osteoclast-like giant cells and a few neoplastic pleomorphic cells. Although extensive histologic sampling was conducted, a classic ductal adenocarcinoma component was not identified. The patient received no further treatment after his surgery and has been doing well with no evidence of recurrence or metastasis for >7 years.

Conclusions: Our results suggest that pure UCOGC has a significantly better prognosis and supports that pure UCOGC may represent a biologically distinct variant of pancreatic carcinoma and it should be separated from other undifferentiated pancreatic carcinomas.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791393PMC
December 2020

Bioengineering Approaches to Accelerate Clinical Translation of Stem Cell Therapies Treating Osteochondral Diseases.

Stem Cells Int 2020 24;2020:8874742. Epub 2020 Dec 24.

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

The osteochondral tissue is an interface between articular cartilage and bone. The diverse composition, mechanical properties, and cell phenotype in these two tissues pose a big challenge for the reconstruction of the defected interface. Due to the availability and inherent regenerative therapeutic properties, stem cells provide tremendous promise to repair osteochondral defect. This review is aimed at highlighting recent progress in utilizing bioengineering approaches to improve stem cell therapies for osteochondral diseases, which include microgel encapsulation, adhesive bioinks, and bioprinting to control the administration and distribution. We will also explore utilizing synthetic biology tools to control the differentiation fate and deliver therapeutic biomolecules to modulate the immune response. Finally, future directions and opportunities in the development of more potent and predictable stem cell therapies for osteochondral repair are discussed.
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http://dx.doi.org/10.1155/2020/8874742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775142PMC
December 2020
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