Publications by authors named "Yu Wu"

842 Publications

Exploring the Safety, Effectiveness, and Cost-Effectiveness of a Chinese Patent Medicine (Fufang E'jiao Syrup) for Alleviating Cancer-Related Fatigue: A Protocol for a Randomized, Double-Blinded, Placebo-Controlled, Multicenter Trial.

Authors:
Zhuo Song Ling-Yun Sun Shan-Shan Gu Xiao-Shu Zhu He-Zheng Lai Fang Lu Ning Cui Qiong-Yang Li Yu Wu Yun Xu

Integr Cancer Ther 2021 Jan-Dec;20:15347354211002919

Xiyuan Hospital of Chinese Academy of Chinese Medical Sciences, Beijing, China.

Objective: To provide higher level evidence on the benefits of a Chinese patent medicine (CPM) (Fufang E'jiao Syrup, FFEJS) for alleviating cancer-related fatigue (CRF), this article describes a protocol for a randomized controlled trial.

Methods/design: We designed a double-blind, placebo-controlled stratified permuted block randomization clinical trial on CRF among 3 types of cancer in China. Participants will be equally allocated to FFEJS group or placebo group according to the randomization sequence and the hospitals they were enrolled at. Each patient will receive 20 ml of either the study formula FFEJS or a placebo formula, 3 times a day for 6 weeks. The follow-up period will be another 4 weeks for safety evaluation. The primary outcome is the difference in improvement of fatigue as measured with the Revised Piper Fatigue Scale-Chinese Version (RPFS-CV). Secondary outcomes include change in fatigue (measured by routine blood panel and hormones in peripheral blood) and QoL (measured by Edmonton symptom assessment scale and Functional Assessment of Cancer Therapy). Patient safety will be measured by liver, renal or cardiac damage, and the risk of FFEJS having a tumor promotion and progression effect will be monitored throughout this study. Cost-effectiveness will also be evaluated mainly by incremental cost per each quality-adjusted life year gained.

Discussion: This article describes the study design of a CPM for CRF in patients with advanced cancer through exploring the effectiveness, safety, and cost-effectiveness of FFEJS.

Trial Registration: ClinicalTrials.gov, NCT04147312. Registered on 1 Sep 2019.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1177/15347354211002919DOI Listing
April 2021

Efficacy of Sanqi (Radix Notoginseng) in treating cerebral hemorrhage in rats with traumatic brain injury.

Authors:
Tao Jiang Xiaqing Zhou Huifang Jiang Rongbiao Ying Zhirong Zhang Danli Cai Yu Wu Haijun Fang Lingcong Wang

J Tradit Chin Med 2021 04;41(2):262-269

Department of Intensive Care Unit, the First Affiliated Rehabilitation Hospital of Zhejiang Chinese Medical University (the First Affiliated Hospital, Zhejiang Chinese Medical University), Hangzhou 310023, China.

Objective: To evaluate the protective efficacy of Sanqi (Radix Notoginseng) on cerebral hemorrhage in a rat model of traumatic brain injury (TBI) by investigating plasminogen activator inhibitor-1 (PAI-1), tissue-type plasminogen activator (t-PA), nuclear factor-κB (NF-κB, p-p65), nitric oxide (NO), endothelin (ET), cluster differentiation (CD61CD62), and coagulation.

Methods: The free-fall method was used to create a rat model of TBI. Forty-eight rats were randomly divided into six groups: the blank group, sham group, model group, low-dose Sanqi (Radix Notoginseng) group, middle-dose Sanqi (Radix Notoginseng) group, and high-dose Sanqi (Radix Notoginseng) group. At 24 h after the model was created, we investigated brain MRI, brain tissue morphology using HE staining, flow cytometry, and immunohistochemical changes.

Results: Cerebral hemorrhage was aggravated in TBI rats (observed in brain specimens, brain MRI, and brain tissue HE). Cerebral immunohistochemistry results demonstrated that the expression of t-PA, PAI-1 and p-p65 increased significantly in TBI rats, while t-PA/PAI-1 had a significant decrease. In addition, CD61CD62, D2D, and ET were significantly increased in TBI rats, and PT and APTT were significantly prolonged; in contrast, NO was significantly decreased. Sanqi (Radix Notoginseng) decreased cerebral hemorrhage in TBI rats (observed in brain MRI and brain tissue HE), and increased t-PA/PAI-1, CD61CD62 significantly. It also significantly decreased the expression of t-PA, PAI-1, and p-p65 in brain immunohistochemistry and significantly decreased PT, APTT, D2D, and ET. However, there were no differences in NO between the model group and the Sanqi (Radix Notoginseng) group.

Conclusion: Sanqi (Radix Notoginseng) can decrease the expression of p-p65, increase t-PA/PAI-1, and stem traumatic intracranial hemorrhage in a TBI rat model.
View Article and Find Full Text PDF

Download full-text PDF

 Source
April 2021

[The Influence of Glutamic Pyruvate Transaminase 2 to Biological Characteristics of Acute Myeloid Leukemia Cell HL-60].

Authors:
Xin-Rong Xiang Qing Li Yan Yu Yu Wu

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):408-415

Department of Hematology and Hematology Research Laboratory, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China,E-mail:

Objective: To investigate the influence of GPT2（glutamic pyruvate transaminase 2）to biological characteristics of human acute myeloid leukemia cell line HL-60.

Methods: The expression of GPT2 in hematological tumor and AML cell was detected. The lentvirus-mediated of short-hairpin RNA (shRNA) was constricted, and the knock-down efficiency of HL-60 in AML cell after infected by lentvirus-mediated was detected by Western blot and Q-PCR. CCK-8 assay and soft agar colony formation assay were used to detect the effect of GPT2 gene deletion to the cell proliferation potential. Fluorescence activated cell sorting(FACS) was used to analyze the effect of gene deletion to the cell cycle and Caspase 3/7 Activity Assay Kit was used to analyze the effect of GPT2 gene deletion to the cell apoptosis.

Results: GPT2 showed mRNA high expression in AML patients. CCK-8, soft agar assay, and Caspase 3/7 Activity Assay Kit results showed that compared with shCtrl group, the cells in shGPT2-1、shGPT2-2、shGPT2-3 group showed the slowing down on proliferation, decreasing on colony ability, and the apoptosis of the cells was increasing significantly. FACS showed that GPT2 gene was related to the cycle of HL-60 cell.

Conclusion: GPT2 appears to involve the proliferation, cycle distribution and apoptosis of AML cell HL-60. The deletion of GPT gene can lead to the inhibitation of cells proliferation and increase apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.016DOI Listing
April 2021

Diagnosis of chronic prostatitis by noninvasive methods in elderly patients with benign prostatic hyperplasia in China.

Authors:
Qifeng Cao Yu Wu Wenbin Guan Yunkai Zhu Jun Qi Ding Xu

Andrologia 2021 Apr 2:e14055. Epub 2021 Apr 2.

Department of Urology, School of Medicine, Xinhua Hospital, Shanghai Jiao Tong University, Shanghai, China.

Chronic prostatitis is hard to be identified in BPH patients in clinical works. This study aimed to diagnose chronic prostatitis in BPH patients by noninvasive methods. BPH patients who received transurethral resection of prostate from January 2014 to July 2015 were enrolled in current study. Patients were received examinations of PSA, sex hormones, inflammatory cytokines, metabolic panel and transrectal ultrasonography. According to histological results, patients were divided into two group of BPH with/without prostatitis. Logistic regression was used to find risk factors of chronic prostatitis. As a result, 181 men with an average age of 72.15 ± 8.41 years were enrolled in this study, including 116 patients with prostatitis and 65 patients without prostatitis. The storage sub-score, PSA and IL-2R were significantly higher in patients with prostatitis than those without prostatitis. Based on logistic regression analysis, the above three parameters were also the risk factors of BPH with prostatitis. The diagnostic model was calculated as: 0.317 × storage sub-score + 0.092 × PSA + 0.003 × IL-2R - 4.296. The AUC was 0.725. Histological prostatitis in BPH patients can be diagnosed by the combination of serum IL-2R, PSA and storage sub-score. Identification of chronic prostatitis in BPH patients could more efficiently alleviate urinary symptoms and reduce the risk of disease progression.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1111/and.14055DOI Listing
April 2021

Relapsed/refractory multiple myeloma-transformed plasma-cell leukemia successfully treated with daratumumab followed by autologous stem cell transplantation.

Authors:
Chen-Lu Yang Neng-Gang Jiang Li Zhang Kai Shen Yu Wu

Ther Adv Hematol 2021 28;12:2040620721989578. Epub 2021 Jan 28.

Department of Hematology and Hematology Research Laboratory, West China Hospital, Sichuan University, Guoxue Alley 37, Chengdu 610041, China.

Daratumumab is a humanized anti-CD38 IgG1 monoclonal antibody which could be used for multiple myeloma (MM). MM with plasma-cell leukemia (PCL) transformation is highly aggressive and is resistant to conventional therapy. Novel therapeutics are needed for PCL, and daratumumab may play role. We report a case of relapsed/refractory multiple myeloma (RRMM)-transformed PCL successfully treated with daratumumab. The case was a 42-year-old man who was diagnosed with MM 2 years ago and relapsed after six cycles of bortezomib-based chemotherapy. The patient rapidly developed hyperleukocytosis and disseminated intravascular coagulation, and was diagnosed with PCL. Daratumumab-based therapy was tried and the case miraculously obtained complete remission (CR) after four doses of a weekly infusion of daratumumab. Finally the patient received autologous hematopoietic stem-cell transplantation (auto-HSCT) and maintained CR. Moreover, we monitored the immune cell dynamics by flow cytometry (FCM) during daratumumab-based treatment. The immune cell subset analysis revealed significant down-regulation of CD38+ natural killer (NK) cells, regulatory T cells (Tregs) and regulatory B cells (Bregs). Meanwhile cytotoxic T-lymphocyte expansion was observed. In conclusion, daratumumab could rapidly decrease tumor burden, improve the condition of the PCL patient, and serve as a bridging salvage chemotherapy for further chimeric antigen recptor T cell therapy (Car-T) or HSCT, which could potentially improve patient survival. The immune cell dynamic findings in this case suggest that the immunomodulatory mechanism may contribute to the antimyeloma effect of daratumumab.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1177/2040620721989578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970699PMC
January 2021

Metastasis of primary breast angiosarcoma to axillary and supraclavicular lymph nodes: a rare case diagnosed using imaging data.

Authors:
Xiaojuan Qin Yu Wu Lan Yu Qing Lv Mingxing Xie

J Int Med Res 2021 Mar;49(3):3000605211002337

Department of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Primary breast angiosarcoma (PBA) is a rare malignant tumor. PBA usually undergoes hematogenous metastasis; lymph node metastasis is very rare in such patients, and metastasis of PBA to the supraclavicular lymph nodes has not previously been reported. Here, we describe a rare case of PBA manifested by a diffuse enlargement of the left breast, with metastasis to the left axillary and bilateral supraclavicular lymph nodes. Contrast-enhanced ultrasound and positron emission tomography findings indicated a malignant lesion, whereas magnetic resonance imaging suggested a benign lesion. Core needle biopsy identified the lesion as a lymphangioma, and the histological characteristics suggested a high-grade angiosarcoma. Multimodal imaging and perfusion patterns obtained using various contrast agents can thus help to diagnose PBA.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1177/03000605211002337DOI Listing
March 2021

No likes, no problem? Users' reactions to the removal of Instagram number of likes on other people's posts and links to body image.

Authors:
Ivanka Prichard Shana O'Toole Yu Wu Jane Harford Marika Tiggemann

Body Image 2021 Mar 27;38:72-79. Epub 2021 Mar 27.

Psychology, College of Education, Psychology & Social Work, Flinders University, Adelaide, South Australia, Australia.

In the age of social media, "likes" are increasingly considered a numerical indicator of popularity and a measure of self-worth. Using an online questionnaire, Australian women (N = 291) aged 18-55 years self-reported their reaction to Instagram removing the visibility of the number of likes from other people's posts, and completed measures of comparison and body image. Findings showed that the majority of participants were in favour of the removal (66.7 %), followed by those who were unsure (23.4 %). Interestingly, those who did not support the removal made significantly greater appearance comparisons based on likes than those who were in favour of the removal of the number of likes. Internalization of the thin ideal mediated the relationship between investment in and comparison with likes and body dissatisfaction. Results also demonstrated that women believe removing the visibility of the number of likes may facilitate a reduction in negative self-focus (comparisons and competitiveness) and improve mental health. Future research is needed to further investigate how the visibility of likes affects women's mental health and body image outcomes in other cultural contexts and across other social media platforms.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1016/j.bodyim.2021.03.007DOI Listing
March 2021

There is no dose-response relationship between allogeneic blood transfusion and healthcare-associated infection: a retrospective cohort study.

Authors:
Yu Lv Qian Xiang Jia Lin Ying Z Jin Ying Fang Hong M Cai Qiong D Wei Hui Wang Chen Wang Jing Chen Jian Ye Caixia Xie Ting L Li Yu J Wu

Antimicrob Resist Infect Control 2021 Mar 29;10(1):62. Epub 2021 Mar 29.

Healthcare-Associated Infection Management Office, Sichuan Academy of Medical Sciences and Sichuan People's Hospital, Chengdu, 610072, Sichuan, People's Republic of China.

Background: The association between allogeneic blood transfusion and healthcare-associated infection (HAI) is considered dose-dependent. However, this association may be confounded by transfusion duration, as prolonged hospitalization stay increases the risk of HAI. Also, it is not clear whether specific blood products have different dose-response risks.

Methods: In this retrospective cohort study, a logistic regression was used to identify confounding factors, and the association between specific blood products and HAI were analyzed. Then Cox regression and restricted cubic spline regression was used to visualize the hazard of HAI per transfusion product.

Results: Of 215,338 inpatients observed, 4.16% were transfused with a single component blood product. With regard to these transfused patients, 480 patients (5.36%) developed a HAI during their hospitalization stay. Logistic regression showed that red blood cells (RBCs) transfusion, platelets transfusion and fresh-frozen plasmas (FFPs) transfusion were risk factors for HAI [odds ratio (OR) 1.893, 95% confidence interval (CI) 1.656-2.163; OR 8.903, 95% CI 6.646-11.926 and OR 1.494, 95% CI 1.146-1.949, respectively]. However, restricted cubic spline regression analysis showed that there was no statistically dose-response relationship between different transfusion products and the onset of HAI.

Conclusions: RBCs transfusion, platelets transfusion and FFPs transfusion were associated with HAI, but there was no dose-response relationship between them.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1186/s13756-021-00928-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008558PMC
March 2021

Silencing of OIP5-AS1 Protects Endothelial Cells From ox-LDL-Triggered Injury by Regulating KLF5 Expression via Sponging miR-135a-5p.

Authors:
Minghu Zhao Yuanyuan Yang Jingchao Li Min Lu Yu Wu

Front Cardiovasc Med 2021 12;8:596506. Epub 2021 Mar 12.

Department of Cardiovascular Comprehensive Ward II, Henan Provincial People's Hospital, Zhengzhou, China.

Long non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of atherosclerosis. LncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been found to be associated with the development of atherosclerosis. In this study, we further investigated the molecular basis of OIP5-AS1 in atherosclerosis pathogenesis. Oxidative low-density lipoprotein (ox-LDL) was used to treat human umbilical vein endothelial cells (HUVECs). The levels of OIP5-AS1, miR-135a-5p, and Krüppel-like factor 5 (KLF5) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell viability, migration, and apoptosis were evaluated using the Cell Counting Kit-8 (CCK-8), Transwell, and flow cytometry, respectively. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and malondialdehyde (MDA) were determined with enzyme-linked immunosorbent assay (ELISA). Targeted interactions among OIP5-AS1, miR-135a-5p, and KLF5 were confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Animal studies were performed to assess the role of OIP5-AS1 in atherosclerosis progression . Our data showed the significant upregulation of OIP5-AS1 in atherosclerosis serum and ox-LDL-stimulated HUVECs. The silencing of OIP5-AS1 protected against ox-LDL-triggered cytotoxicity in HUVECs and diminished lipids secretion in ApoE mice. Moreover, OIP5-AS1 functioned as a molecular sponge of miR-135a-5p, and miR-135a-5p was a functional mediator of OIP5-AS1 in regulating ox-LDL-induced HUVEC injury. KLF5 was a direct target of miR-135a-5p, and the increased expression of miR-135a-5p alleviated ox-LDL-induced cytotoxicity by downregulating KLF5. Furthermore, OIP5-AS1 influenced KLF5 expression through sponging miR-135a-5p. The current work identified that the silencing of OIP5-AS1 protected against ox-LDL-triggered cytotoxicity in HUVECs at least in part by influencing KLF5 expression via acting as a miR-135a-5p sponge.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.3389/fcvm.2021.596506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994260PMC
March 2021

Use of Three-Dimensional Arterial Spin Labeling to Evaluate Renal Perfusion in Patients With Chronic Kidney Disease.

Authors:
Fang Lu Jing Yang Shuohui Yang Kuehn Bernd Caixia Fu Chenyao Yang Huihui Xu Mengxiao Liu Songhua Zhan Chen Wang Rongfang Guo Yu Wu

J Magn Reson Imaging 2021 Mar 26. Epub 2021 Mar 26.

Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: A noninvasive method for evaluating renal blood flow (RBF) in patients with chronic kidney disease (CKD) may have clinical value in disease staging, management, and prognostication.

Purpose: To evaluate effectiveness of three-dimensional pseudocontinuous arterial spin labeling (pCASL) and pulsed arterial spin labeling (PASL) in assessment of cortex and outer medulla (cortex/OM) RBF in CKD patients and healthy volunteers (HVs).

Study Type: Prospective, in a single institution.

Subjects: A total of 48 CKD patients (stage 1, 2, 3, and 4-5: N = 11, 12, 13, and 12, respectively) and 18 HVs FIELD STRENGTH/SEQUENCE: 3 T, pCASL, and PASL with a three-dimensional hybrid gradient echo/spin echo sequence.

Assessment: Quality of RBF images derived from pCASL and PASL were evaluated and RBF in cortex/OM measured. Clinical and laboratory data were recorded.

Statistical Tests: Image quality differences between pCASL and PASL were evaluated with Wilcoxon signed-rank test. For both methods, analysis of variance, followed by Fisher's LSD-t test, was used to determine whether RBF differed between CKD stages and HVs. Pearson correlation coefficients were calculated to assess strength of relationships between cortex/OM RBF and data from clinical and laboratory tests.

Results: Image quality differences were significantly higher in pCASL than PASL in both patients and HVs (both P < 0.05). For pCASL, cortex/OM RBF of patients were significantly lower than those of HVs (P < 0.05). Cortex/OM RBF were higher in S1 and S2 patients than those in S3 and S4-5 (P < 0.05). For PASL, only RBF in cortex of S1 and S2 patients were significantly higher than those of S4-5 (P < 0.05). Good correlations between pCASL RBF and estimated glomerular filtration (eGFR) were found in cortex/OM of patients (rho = 0.796 and 0.798, respectively, both P < 0.05), higher than those between PASL RBF and eGFR (rho = 0.430 and 0.374, respectively, both P < 0.05).

Data Conclusion: Three-dimensional pCASL may potentially be a noninvasive technique to assess renal perfusion in CKD patients in different stages.

Level Of Evidence: 1 TECHNICAL EFFICACY: Stage 2.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1002/jmri.27609DOI Listing
March 2021

Progressive Transfer Learning for Face Anti-Spoofing.

Authors:
Ruijie Quan Yu Wu Xin Yu Yi Yang

IEEE Trans Image Process 2021 30;30:3946-3955. Epub 2021 Mar 30.

Face anti-spoofing (FAS) techniques play an important role in defending face recognition systems against spoofing attacks. Existing FAS methods often require a large number of annotated spoofing face data to train effective anti-spoofing models. Considering the attacking nature of spoofing data and its diverse variants, obtaining all the spoofing types in advance is difficult. This would limit the performance of FAS networks in practice. Thus, an online learning FAS method is highly desirable. In this paper, we present a semi-supervised learning based framework to tackle face spoofing attacks with only a few labeled training data (e.g.,  ∼  50 face images). Specifically, we progressively adopt the unlabeled data with reliable pseudo labels during training to enrich the variety of training data. We observed that face spoofing data are naturally presented in the format of video streams. Thus, we exploit the temporal consistency to consolidate the reliability of a pseudo label for a selected image. Furthermore, we propose an adaptive transfer mechanism to ameliorate the influence of unseen spoofing data. Benefiting from the progressively-labeling nature of our method, we are able to train our network on not only data of seen spoofing types (i.e., the source domain) but also unlabeled data of unseen attacking types (i.e., the target domain). In this way, our method can reduce the domain gap and is more practical in real-world anti-spoofing scenarios. Extensive experiments in both the intra-database and inter-database scenarios demonstrate that our method is on par with the state-of-the-art methods but employs remarkably less labeled data (less than 0.1% labeled spoofing data in a dataset). Moreover, our method significantly outperforms fully-supervised methods on cross-domain testing scenarios with the help of our progressive learning fashion.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1109/TIP.2021.3066912DOI Listing
April 2021

Development of a Genomic Signatures-Based Predictor of Initial Platinum-Resistance in Advanced High-Grade Serous Ovarian Cancer Patients.

Authors:
Yuan Li Xiaolan Zhang Yan Gao Chunliang Shang Bo Yu Tongxia Wang Junyan Su Cuiyu Huang Yu Wu Hongyan Guo Chunfang Ha

Front Oncol 2020 5;10:625866. Epub 2021 Mar 5.

Department of Gynecology and Obstetrics Department, General Hospital of Ningxia Medical University, Yinchuan, China.

Background: High grade serous ovarian cancer (HGSOC) is the most common subtype of ovarian cancer. Although platinum-based chemotherapy has been the cornerstone for HGSOC treatment, nearly 25% of patients would have less than 6 months of interval since the last platinum chemotherapy, referred to as platinum-resistance. Currently, no precise tools to predict platinum resistance have been developed yet.

Methods: Ninety-nine HGSOC patients, who have finished cytoreductive surgery and platinum-based chemotherapy in Peking University Third Hospital from 2018 to 2019, were enrolled. Whole-genome sequencing (WGS) and whole-exome sequencing (WES) were performed on the collected tumor tissue samples to establish a platinum-resistance predictor in a discovery cohort of 57 patients, and further validated in another 42 HGSOC patients.

Results: A high prevalence of alterations in DNA damage repair (DDR) pathway, including , was identified both in the platinum-sensitive and resistant HGSOC patients. Compared with the resistant subgroup, there was a trend of higher prevalence of homologous recombination deficiency (HRD) in the platinum-sensitive subgroup (78.95% vs. 47.37%, p=0.0646). Based on the HRD score, microhomology insertions and deletions (MHID), copy number changes load, duplication load of 1-100 kb, single nucleotide variants load, and eight other mutational signatures, a combined predictor of platinum-resistance, named as DRDscore, was established. DRDscore outperformed in predicting the platinum-sensitivity than the previously reported biomarkers with a predictive accuracy of 0.860 at a threshold of 0.7584. The predictive performance of DRDscore was validated in an independent cohort of 42 HGSOC patients with a sensitivity of 90.9%.

Conclusions: A multi-genomic signature-based analysis enabled the prediction of initial platinum resistance in advanced HGSOC patients, which may serve as a novel assessment of platinum resistance, provide therapeutic guidance, and merit further validation.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.3389/fonc.2020.625866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977004PMC
March 2021

Organoids as a new model system to study neural tube defects.

Authors:
Yu Wu Sisi Peng Richard H Finnell Yufang Zheng

FASEB J 2021 Apr;35(4):e21545

Department of Cellular and Developmental Biology, School of life sciences, Fudan University, Shanghai, China.

The neural tube is the first critically important structure that develops in the embryo. It serves as the primordium of the central nervous system; therefore, the proper formation of the neural tube is essential to the developing organism. Neural tube defects (NTDs) are severe congenital defects caused by failed neural tube closure during early embryogenesis. The pathogenesis of NTDs is complicated and still not fully understood even after decades of research. While it is an ethically impossible proposition to investigate the in vivo formation process of the neural tube in human embryos, a newly developed technology involving the creation of neural tube organoids serves as an excellent model system with which to study human neural tube formation and the occurrence of NTDs. Herein we reviewed the recent literature on the process of neural tube formation, the progress of NTDs investigations, and particularly the exciting potential to use neural tube organoids to model the cellular and molecular mechanisms underlying the etiology of NTDs.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1096/fj.202002348RDOI Listing
April 2021

Calcified chondroid mesenchymal neoplasms with FN1-receptor tyrosine kinase gene fusions including FGFR2, FGFR1, MERTK, NTRK1, and TEK: a molecular and clinicopathologic analysis.

Authors:
Yajuan J Liu Wenjing Wang Jeffrey Yeh Yu Wu Jose G Mantilla Christopher D M Fletcher Robert W Ricciotti Eleanor Y Chen

Mod Pathol 2021 Mar 16. Epub 2021 Mar 16.

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, United States.

Translocations involving FN1 have been described in a variety of neoplasms that share the presence of a cartilage matrix and may also contain a variable extent of calcification. Fusions of FN1 to FGFR1 or FGFR2 have been reported in nine soft tissue chondromas, mostly demonstrated indirectly by FISH analysis. Delineation of FN1 fusions with various partner genes will facilitate our understanding of the pathogenesis and diagnostic classification of these neoplasms. In this study, we present molecular, clinical, and pathologic features of 12 cartilaginous soft tissue neoplasms showing a predilection for the TMJ region and the distal extremities. We analyzed for gene fusions with precise breakpoints using targeted RNA-seq with a 115-gene panel. We detected gene fusions in ten cases, including three novel fusions, FN1-MERTK, FN1-NTRK1, and FN1-TEK, each in one case, recurrent FN1-FGFR2 fusion in five cases, FN1-FGFR1 in one case, and FGFR1-PLAG1 in one case. The breakpoints in the 5' partner gene FN1 ranged from exons 11-48, retaining the domains of a signal peptide, FN1, FN2, and/or FN3, while the 3' partner genes retained the transmembrane domain, tyrosine kinase (TK) domains, and/or Ig domain. The tumors are generally characterized by nodular/lobular growth of polygonal to stellate cells within a chondroid matrix, often accompanied by various patterns of calcification, resembling those described for the chondroblastoma-like variant of soft tissue chondroma. Additional histologic findings include extensive calcium pyrophosphate dihydrate deposition in two cases and features resembling tenosynovial giant cell tumor (TGCT). Overall, while the tumors from our series show significant morphologic overlap with chondroblastoma-like soft tissue chondroma, we describe findings that expand the morphologic spectrum of these neoplasms and therefore refer to them as "calcified chondroid mesenchymal neoplasms." These neoplasms represent a spectrum of chondroid/cartilage matrix-forming tumors harboring FN1-receptor TK fusions that include those classified as soft tissue chondroma as well as chondroid TGCT.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1038/s41379-021-00786-xDOI Listing
March 2021

Effects of High and Low Salt Concentrations in Electrolytes at Lithium-Metal Anode Surfaces Using DFT-ReaxFF Hybrid Molecular Dynamics Method.

Authors:
Yue Liu Qintao Sun Peiping Yu Yu Wu Liang Xu Hao Yang Miao Xie Tao Cheng William A Goddard

J Phys Chem Lett 2021 Mar 16;12(11):2922-2929. Epub 2021 Mar 16.

Materials and Process Simulation Center, California Institute of Technology, Pasadena, California 91125, United States.

Due to creating a passivated solid electrolyte interphase (SEI), high concentration (HC) electrolytes demonstrate peculiar physicochemical properties and outstanding electrochemical performance. However, the structures of such SEI remains far from clear. In this work, a ybrid and eactive molecular dynamics (HAIR) scheme is employed to investigate the concentration effect of SEI formation by simulating the reductive degradation reactions of lithium bis(fluorosulfonyl)imide (LiFSI) in 1,3 dioxalane (DOL) electrolytes at concentrations of 1 M, 4 M, and 10 M. The efficient HAIR scheme allows the simulations to reach 1 ns to predict electrolytes' deep products at different concentrations. The simulation findings show that the most critical distinction between HC and its low concentration (LC) analogue is that anion decomposition in HC is much more incomplete when only S-F breaking is observed. These insights are important for the future development of advanced electrolytes by rational design of electrolytes.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1021/acs.jpclett.1c00279DOI Listing
March 2021

Reducing Blood Loss by Changing to Small Volume Tubes for Laboratory Testing.

Authors:
Yu Wu Aaron C Spaulding Shalmali Borkar Michelle M Shoaei Maria Mendoza Rhonda L Grant Bruce W Barber Gretchen S Johns Pablo Moreno Franco

Mayo Clin Proc Innov Qual Outcomes 2021 Feb 19;5(1):72-83. Epub 2020 Nov 19.

Department of Transplant, Mayo Clinic, Jacksonville, FL.

Objective: To reduce diagnostic blood loss by using small volume tubes for routine laboratory testing throughout the hospital, as blood loss from laboratory testing can be substantial for patients and may lead to hospital-acquired anemia.

Patients And Methods: Diagnostic blood loss was evaluated in hospitalized patients between April 1, 2017, and June 1, 2018. The preintervention, during intervention, and postintervention mean diagnostic blood loss per hospitalized patient was compared across the floors and for each type of tube for hematology, basic metabolic panel, and coagulation tests. Mean hemoglobin levels, blood transfusions per hospitalized patient, and percent redraws were also compared.

Results: The total volume of blood drawn for all the 3 tests decreased across each implementation phase; however, only patients admitted to the transplant and critical care (T/CC) units had increased hemoglobin levels. In addition, there was a significant reduction in transfusions across implementation phases. The incidence risk ratio for transfusion reduced even more in patients admitted to the T/CC units. Finally, there was no significant difference in the overall percent redraws across all the units.

Conclusion: The use of small volume tubes in exchange for standard sized tubes markedly decreased diagnostic blood loss by 25.7% in all the units and 22.9% in the T/CC units. Also, the number of transfusions decreased across units, with the greatest decrease in the T/CC units. An increase in mean hemoglobin levels was observed specifically in patients admitted to the T/CC units, with no corresponding change in percent redraws across all the units.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1016/j.mayocpiqo.2020.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930757PMC
February 2021

Non-muscle myosin II knockdown improves survival and therapeutic effects of implanted bone marrow-derived mesenchymal stem cells in lipopolysaccharide-induced acute lung injury.

Authors:
Guosheng Wu Fei Chang He Fang Xingfeng Zheng Mingzhu Zhuang Xiaobin Liu Wenjia Hou Long Xu Zhengli Chen Chenqi Tang Yu Wu Yu Sun Feng Zhu

Ann Transl Med 2021 Feb;9(3):262

Department of Burn Surgery, Changhai Hospital, Naval Medical University, Shanghai, China.

Background: Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to have some beneficial effects in acute lung injury (ALI), but the therapeutic effects are limited due to apoptosis or necrosis after transplantation into injured lungs. Here, we aim to explore whether Non-muscle myosin II (NM-II) knockdown could enhance BMSCs survival and improve therapeutic effects in ALI.

Methods: MSCs, isolated from rat bone marrow, were transfected with the small interfering RNA (siRNA) targeted to NM-II mRNA by a lentivirus vector. Rats were equally randomized to four groups: the control group was given normal saline via tail vein; the other three groups underwent intratracheal lipopolysaccharide (LPS) instillation followed by administration with either normal saline, BMSCs transduced with lentivirus-enhanced green fluorescent protein (eGFP) empty vector, or BMSCs transduced with lentivirus-eGFP NM-II siRNA. Hematoxylin and eosin staining was used to evaluate lung histopathologic changes and Masson trichrome staining was used to assess lung fibrosis. The myeloperoxidase activity was also tested in lung tissues. The mRNA expression of inflammatory cytokines in lung tissues was determined via quantitative reverse transcription PCR. Sex-determining region of the Y chromosome gene expression was measured by fluorescence in situ hybridization (FISH) assay. The expression of self-renewal activity and apoptosis-associated proteins were measured by Western blot.

Results: Transplantation of NM-II siRNA-modified BMSCs could improve histopathological morphology, decrease inflammatory infiltrates, down-regulate the expression levels of inflammatory cytokines, and reduce pulmonary interstitial edema. NM-II siRNA-modified BMSCs showed antifibrotic properties and alleviated the degrees of pulmonary fibrosis induced by endotoxin. In addition, NM-II knockdown BMSCs showed slightly better therapeutic effect on lung inflammation when compared with control BMSCs. The beneficial effects of NM-II siRNA-modified BMSCs may be attributed to enhanced self-renewal activity and decreased apoptosis.

Conclusions: NM-II knockdown could inhibit the apoptosis of implanted BMSCs in lung tissues and improve its self-renewal activity. NM-II siRNA-modified BMSCs have a slightly enhanced ability to attenuate lung injury after LPS challenge.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.21037/atm-20-4851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940885PMC
February 2021

Formation pattern and regulatory mechanisms of pollen wall in Arabidopsis.

Authors:
Xiaofeng Ma Yu Wu Genfa Zhang

J Plant Physiol 2021 Feb 12;260:153388. Epub 2021 Feb 12.

Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address:

In angiosperms, mature pollen is wrapped by a pollen wall, which is important for maintaining pollen structure and function. Pollen walls provide protection from various environmental stresses and preserve pollen germination and pollen tube growth. The pollen wall structure has been described since pollen ultrastructure investigations began in the 1960s. Pollen walls, which are the most intricate cell walls in plants, are composed of two layers: the exine layer and intine layer. Pollen wall formation is a complex process that occurs via a series of biological events that involve a large number of genes. In recent years, many reports have described the molecular mechanisms of pollen exine development. The formation process includes the development of the callose wall, the wavy morphology of primexine, the biosynthesis and transport of sporopollenin in the tapetum, and the deposition of the pollen coat. The formation mechanism of the intine layer is different from that of the exine layer. However, few studies have focused on the regulatory mechanisms of intine development. The primary component of the intine layer is pectin, which plays an essential role in the polar growth of pollen tubes. Demethylesterified pectin is mainly distributed in the shank region of the pollen tube, which can maintain the hardness of the pollen tube wall. Methylesterified pectin is mainly located in the top region, which is beneficial for improving the plasticity of the pollen tube top. In this review, we summarize the developmental process of the anther, pollen and pollen wall in Arabidopsis; furthermore, we describe the research progress on the pollen wall formation pattern and its molecular mechanisms in detail.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1016/j.jplph.2021.153388DOI Listing
February 2021

[Advances in Immunotherapy for Drug Addiction].

Authors:
Lin Chen Qin Ru Mei Zhou Yu Xiang Wu

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Feb;43(1):116-123

School of Physical Education, School of Medicine,Jianghan University,Wuhan 430056,China.

Drug addiction is a major worldwide medical and social problem.Cocaine,nicotine,methamphetamine,heroin and other psychoactive substances,with small molecular weight,can easily cross the blood-brain barrier and eventually lead to addiction and other serious neuropsychological damage.There is no effective cure for addiction currently.The drug-antibody complex formed on the basis of active or passive immunotherapy could not cross the blood-brain barrier,which reduces the concentration of the free active drug and prevents its distribution in the brain,thereby weakening the drug addiction-related reward effects.It provides a promising way for the treatment of drug addiction.This article reviews the progress of immunotherapy against psychoactive substances such as cocaine,nicotine,methamphetamine and heroin in the past 50 years from the aspects of active immunity,passive immunity,drug metabolism-related enzymes,adjuvants and so on.The goal is to provide some ideas for the development of agents for the treatment of psychoactive substance addiction.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.3881/j.issn.1000-503X.11591DOI Listing
February 2021

Breastfeeding competency scale (BCS); development and validation of an evaluation instrument on breastfeeding competency in third trimester pregnancy.

Authors:
Yu Wu Ying Wang Jiazhen Hu Yan Dang Yuanyuan Zhang Xiumei Qi Qingxiu Tian Aihua Wang Yunfeng Li

BMC Pregnancy Childbirth 2021 Mar 4;21(1):179. Epub 2021 Mar 4.

Department of Nursing, Shandong Provincial Qianfoshan Hospital, 16766 Jingshi Street, Jinan, 250014, Shandong province, China.

Background: Breastfeeding plays an important role in the early stages of humans and throughout the development process. Breastfeeding competency is a self-assessment of pregnant women's overall competency to breastfeeding which could predict the breastfeeding behaviours of pregnant women. However, a valid and reliable scale for assessing breastfeeding competency has not yet been developed and validated. This study was conducted to develop and validate an assessment scale designed to assess pregnant women's breastfeeding competency in the third trimester: the Breastfeeding Competency Scale (BCS).

Methods: The BCS was developed and validated over three phases between September 2018 and September 2019, and these phases included item statistical analysis, exploratory factor analysis (EFA), content validation, internal consistency assessment, split-half reliability assessment and confirmatory factor analysis (CFA).

Results: The item statistical analysis and EFA resulted in 38 items and 4 factors that explained 66.489% of the total variance. The Cronbach's α coefficients for the total scale and the 4 factors were 0.970, 0.960, 0.940, 0.822 and 0.931. The split-half reliability of the BCS was 0.894 and 0.890. CFA model showed that the 4-factor model fits the data well.

Conclusions: The BCS is a new valid and reliable instrument for assessing the breastfeeding competency of pregnant women in the third trimester.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1186/s12884-021-03664-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934416PMC
March 2021

Statins use and the prognosis of colorectal cancer: a meta-analysis.

Authors:
Liusheng Li Ning Cui Tengteng Hao Jianhua Zou Wu Jiao Kangjun Yi Wu Yu

Clin Res Hepatol Gastroenterol 2021 Mar 1;45(5):101588. Epub 2021 Mar 1.

Department of Oncology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. Electronic address:

Background: Previous observational studies regarding the prognostic value of statin on colorectal cancer (CRC) patients showed various results.

Methods: Articles regarding the prognostic value of statin on CRC and published in English and before October 2020 were searched in the following databases: PubMed, Web of Science, EMBASE, Medline and Google Scholar. The multivariate hazard ratios (HRs) and their 95% confidence intervals (CI) were computed to explore associations between statins use and overall mortality or cancer-specific mortality of CRC.

Results: The study included 5 retrospective case-control studies (including 475 statins users and 1925 no-statin users) and 11 prospective cohort studies (including 40659 statins users and 344459 no-statin users). The present study showed that statins use might be significantly associated with lower overall mortality in CRC with a random effects model (HR = 0.81, 95% CI 0.76 to 0.86, I = 61.9%, p value for Q test <0.001). In addition, statins use might be significantly associated with lower cancer-specific mortality in CRC with a random effects model (HR = 0.78, 95% CI 0.72 to 0.85, I = 57.3%, p value for Q test = 0.007).

Conclusions: In conclusion, the present study indicated that that statin use was a protective factor for CRC prognosis. However, the relationship between statins use and CRC prognosis requires repeated and large prospective studies to be verified.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1016/j.clinre.2020.101588DOI Listing
March 2021

Application of Magnetic Resonance Imaging in the Evaluation of Disease Activity in Graves' Ophthalmopathy.

Authors:
Xingtong Liu Yun Su Mengda Jiang Sijie Fang Yazhuo Huang Yinwei Li Sisi Zhong Yang Wang Shuo Zhang Yu Wu Jing Sun Xianqun Fan Huifang Zhou

Endocr Pract 2021 Mar 15;27(3):198-205. Epub 2020 Dec 15.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China, 200011; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China, 200011. Electronic address:

Objective: To evaluate magnetic resonance imaging parameters, T2 signal intensity ratios (SIRs), and normalized apparent diffusion coefficients (n-ADC) of the extraocular muscles (EOMs) in the identification of different stages of Graves' ophthalmopathy (GO) and to find out the correlation of T2-SIRs and n-ADC values with disease changes after anti-inflammatory treatment.

Methods: Altogether, 43 patients (86 orbits) were enrolled and classified into "active" or "inactive" stages by clinical activity score (CAS). Twenty-three (53.5%) patients received anti-inflammatory treatment and underwent a follow-up evaluation. Fifteen age- and gender-matched control participants (30 orbits) were included. T2-SIRs and n-ADC values of EOMs were calculated among GO and healthy controls and were correlated with CAS. Changes in these parameters were also evaluated before and after anti-inflammatory treatment.

Results: Mean T2-SIRs and n-ADC values were both significantly higher in GO patients than in controls and higher in active GO than in inactive GO. In the inactive stage, n-ADC values of inferior rectus muscles were still higher than those in healthy controls. Both T2-SIRs and n-ADC values decreased after intravenous steroid pulse therapy. The cutoff value of pretreatment n-ADC was 1.780 to detect stages with specificity of 93.7% and sensitivity of 48.3% (P = .035).

Conclusion: T2-SIRs and n-ADC values are valuable magnetic resonance imaging indicators of the inflammatory activity in GO by detecting involvement of EOMs. They are also ideal tools to monitor the efficacy of anti-inflammatory treatment in patients with active stage GO. n-ADC values, when combined with CAS, can be promising predictive factors in the detection of stages of diseases.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1016/j.eprac.2020.09.008DOI Listing
March 2021

Protoplast fusion between Blakeslea trispora 14,271 (+) and 14,272 (-) enhanced the yield of lycopene and β-carotene.

Authors:
Yanlong Wang Yicun Wang Xin Chen Na Gao Yu Wu Hongfa Zhang

World J Microbiol Biotechnol 2021 Mar 2;37(4):58. Epub 2021 Mar 2.

Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, 272067, China.

Blakeslea trispora, a heterothallic Zygomycota with two mating types (termed "plus" and "minus"), is an ideal source of lycopene and β-carotene. The lycopene and β-carotene yields when the two type strains are used for fermentation separately are lower than those when they are joint together. To enhance the yield of lycopene and β-carotene in B. trispora, protoplast fusion technology was carried out between ATCC 14,271 (+) and ATCC 14,272 (-). After protoplast preparation, protoplast fusion, fusion sorting, fusion regeneration, and high-throughput screening, two fusions (Fu-1and Fu-2) with high lycopene and β-carotene yields were obtained. The lycopene yields of Fu-1 and Fu-2 were increased to 0.60 mg/gDW and 0.90 mg/gDW, which were respectively 3.62- and 5.44-fold those of 14,271 and 1.76- and 2.64-fold those of 14,272. The β-carotene yields of Fu-1 and Fu-2 were increased to 22.07 mg/gDW and 36.93 mg/gDW, which were respectively 1.72- and 2.89-fold those of 14,271 and 1.23- and 2.06-fold those of 14,272. In this study, the protoplast fusion technique was successfully used in Blakeslea trispora, providing new ideas for improving lycopene and β-carotene production.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1007/s11274-021-03023-4DOI Listing
March 2021

Identifying Visible Parts via Pose Estimation for Occluded Person Re-Identification.

Authors:
Jiaxu Miao Yu Wu Yi Yang

IEEE Trans Neural Netw Learn Syst 2021 Mar 2;PP. Epub 2021 Mar 2.

We focus on the occlusion problem in person re-identification (re-id), which is one of the main challenges in real-world person retrieval scenarios. Previous methods on the occluded re-id problem usually assume that only the probes are occluded, thereby removing occlusions by manually cropping. However, this may not always hold in practice. This article relaxes this assumption and investigates a more general occlusion problem, where both the probe and gallery images could be occluded. The key to this challenging problem is depressing the noise information by identifying bodies and occlusions. We propose to incorporate the pose information into the re-id framework, which benefits the model in three aspects. First, it provides the location of the body. We then design a Pose-Masked Feature Branch to make our model focus on the body region only and filter those noise features brought by occlusions. Second, the estimated pose reveals which body parts are visible, giving us a hint to construct more informative person features. We propose a Pose-Embedded Feature Branch to adaptively re-calibrate channel-wise feature responses based on the visible body parts. Third, in testing, the estimated pose indicates which regions are informative and reliable for both probe and gallery images. Then we explicitly split the extracted spatial feature into parts. Only part features from those commonly visible parts are utilized in the retrieval. To better evaluate the performances of the occluded re-id, we also propose a large-scale data set for the occluded re-id with more than 35 000 images, namely Occluded-DukeMTMC. Extensive experiments show our approach surpasses previous methods on the occluded, partial, and non-occluded re-id data sets.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1109/TNNLS.2021.3059515DOI Listing
March 2021

The prognostic significance of single-nucleotide polymorphism array-based whole-genome analysis and uniparental disomy in myelodysplastic syndrome.

Authors:
Yang Ou Yan Yang Hongbin Yu Xin Zhang Min Liu Yu Wu

Int J Lab Hematol 2021 Mar 2. Epub 2021 Mar 2.

Department of Hematology and Hematology Research Laboratory, West China Hospital, Sichuan University, Chengdu, China.

Introduction: Myelodysplastic syndrome (MDS) is a group of heterogeneous hematological diseases characterized by ineffective hematopoiesis and dysplastic morphology. Single nucleotide polymorphism array (SNP-A)-based whole genome analysis has a much higher resolution for chromosomal alterations when compared with conventional cytogenetic tools. In the present study, we evaluated the diagnostic value and prognostic significance of SNP-A in MDS patients with normal karyotypes.

Methods: A total of 127 patients with MDS and myeloproliferative neoplasms or acute myeloid leukemia with myelodysplasia-related changes were included in our study. The advantages and disadvantages of SNP-A were compared with those of traditional metaphase cytogenetic analysis (MC). The Kaplan-Meier analysis and COX regression analysis were used to investigate the prognostic value of SNP-A and uniparental disomy (UPD) in MDS patients with normal karyotype. Furthermore, the chromosomal abnormalities detected by SNP-A in patients with specific gene mutations were explored.

Results: SNP-A was more sensitive toward meaningful chromosomal aberrations (58.2% vs 36.9%; P < .05) than MC. Among the patients with normal karyotype, those who were detected with new chromosomal abnormalities via SNP-A presented with inferior survival compared with those without the abnormalities (P = .003). Additionally, the presence of UPD was an independent prognostic factor in patients with normal karyotype (P = .01). TP53 and RUNX1 mutations often occurred with abnormalities in chromosomes 17p and 21q, respectively.

Conclusions: Compared with MC, SNP-A capable of detecting UPD can offer more diagnostic and prognostic information; TP53 and RUNX1 gene mutations are often accompanied by abnormalities in their chromosomes (17p, 22q).
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1111/ijlh.13502DOI Listing
March 2021

Corrigendum: Dual-Targeting Nanoparticle-Mediated Gene Therapy Strategy for Hepatocellular Carcinoma by Delivering Small Interfering RNA.

Authors:
Qi Chang Zheng Shuai Jiang Yu Zhe Wu Dan Shang Yong Zhang Shao Bo Hu Xiang Cheng Chen Zhang Ping Sun Yang Gao Zi Fang Song Min Li

Front Bioeng Biotechnol 2021 11;9:656268. Epub 2021 Feb 11.

Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.3389/fbioe.2020.00512.].
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.3389/fbioe.2021.656268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906225PMC
February 2021

Immunological Features of Paranasal Sinus Mucosa in Patients with Graves' Orbitopathy.

Authors:
Yi Lu Yu Wu Yazhuo Huang Sijie Fang Yinwei Li Jing Sun Huifang Zhou

Front Endocrinol (Lausanne) 2020 10;11:621321. Epub 2021 Feb 10.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.

Background: Previous studies showed that patients with Graves' orbitopathy (GO) had concomitant mucosal abnormality within the paranasal sinuses. It remains unknown whether the immunological reactions in sinus mucosa affect the orbit inflammation in GO.

Methods: Patients with GO underwent sinus computed tomography (CT) scans for sinus mucosal disease by two independent reviewers using the Lund-MacKay systems. Ethmoid mucosal samples were collected during orbital decompression surgeries for patients with GO and correction surgeries for patients with old orbital fractures as controls. Histological analysis and immunofluorescence were performed in all sinus mucosa tissues. Flow cytometry analysis was used to examine the immunological features of sinus mucosa in both GO and control groups.

Results: Immunohistochemistry showed that the paranasal sinus mucosa of patients with GO grew swelling, with goblet cell and small vessel proliferation, endothelial cell swelling, and inflammatory cell infiltration. The number of T helper (Th)1, Th17, and gamma-delta T cells in nasal sinus mucosa of patients with GO increased significantly compared with those from controls. Further, the proportion of Th1 cells was significantly correlated with clinical activity score. In addition, there was a decreased number of regulatory T cells in patients with GO. The number of Th2 cells showed no significant difference between the two groups. Finally, the proportion of interleukin-22-producing cell subsets in gamma-delta T cells of patients with GO was significantly increased compared with those from controls.

Conclusions: Our observations illustrated a potential pathogenic role of mucosal-infiltrating T cells, which may have the possibility to aggravate inflammatory responses in GO.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.3389/fendo.2020.621321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902916PMC
February 2021

Plasticity of Treg and imbalance of Treg/Th17 cells in patients with systemic sclerosis modified by FK506.

Authors:
Xinjuan Liu Yu Wu Mengtao Li Jianyu Hao Qian Wang Xiaofeng Zeng

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:2058738421998086

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

To determine the effects of Tacrolimus (FK506) on Treg cells and subpopulations in SSc patients and assess the ability of FK506 to modify the immune imbalance of Treg/Th17 cells. We analyzed PBMC from five SSc patients and six healthy control by flow cytometry after cultured with 0, 0.1, 1, or 10 ng/ml FK506 in vitro. The number of Treg cells decreased in SSc patients treated with FK506. The number of FrI cells were decreased in SSc following FK506 treatment. The drug did increase the frequency of FrII/Treg cells, but not FrII cells. However, FK506 significantly decreased FrIII in both SSc patients and controls. FK506 clearly decreased the numbers of Th17 cells and FoxP3IL-17 cells. The proliferation capacity of cells was also inhibited by FK506, which had a greater effect on FoxP3 cells than FoxP3 cells. FK506 did inhibit the proliferation of FrIII cells, but not FrI or FrII cells. Our study provides that FK506 reduced the number of FoxP3 CD45RA T cells (FrIII) by inhibiting its proliferation. Therefore, FK506 modifies Treg cells and the immune imbalance between Tregs and Th17 cells in SSc patients.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1177/2058738421998086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917869PMC
February 2021

Thoracic shape changes in newborns due to their position.

Authors:
Serena de Gelidi Andy Bardill Nima Seifnaraghi Yu Wu Andreas Demosthenous Marika Rahtu Merja Kallio Richard Bayford

Sci Rep 2021 Feb 24;11(1):4446. Epub 2021 Feb 24.

Faculty of Science & Technology, Middlesex University, London, UK.

The highly compliant nature of the neonatal chest wall is known to clinicians. However, its morphological changes have never been characterized and are especially important for a customised monitoring of respiratory diseases. Here, we show that a device applied on newborns can trace their chest boundary without the use of radiation. Such technology, which is easy to sanitise between patients, works like a smart measurement tape drawing also a digital cross section of the chest. We also show that in neonates the supine position generates a significantly different cross section compared to the lateral ones. Lastly, an unprecedented comparison between a premature neonate and a child is reported.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.1038/s41598-021-83869-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904854PMC
February 2021

Fine-Tuning Pyridinic Nitrogen in Nitrogen-Doped Porous Carbon Nanostructures for Boosted Peroxidase-Like Activity and Sensitive Biosensing.

Authors:
Hongye Yan Linzhe Wang Yifeng Chen Lei Jiao Yu Wu Weiqing Xu Wenling Gu Weiyu Song Dan Du Chengzhou Zhu

Research (Wash D C) 2020 6;2020:8202584. Epub 2020 Nov 6.

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China.

Carbon materials have been widely used as nanozymes in bioapplications, attributing to their intrinsic enzyme-like activities. Nitrogen (N)-doping has been explored as a promising way to improve the activity of carbon material-based nanozymes (CMNs). However, hindered by the intricate N dopants, the real active site of N-doped CMNs (N-CMNs) has been rarely investigated, which subsequently retards the further progress of high-performance N-CMNs. Here, a series of porous N-CMNs with well-controlled N dopants were synthesized, of which the intrinsic peroxidase (POD)like activity has a positive correlation with the pyridinic N content. Density functional theory calculations also reveal that pyridinic N boosts the intrinsic POD-like activity of N-CMNs. Pyridinic-N dopant can effectively promote the first HO desorption process in comparison with the graphitic and pyrrolic N, which is the key endothermic reaction during the catalytic process. Then, utilizing the optimized nanozymes with high pyridinic N content (N-CMNs) and superior POD-like activity, a facile total antioxidant capacity (TAC) assay was developed, holding great promise in the quality assessment of medicine tablets and antioxidant food for healthcare and healthy diet.
View Article and Find Full Text PDF

Download full-text PDF

 Source
http://dx.doi.org/10.34133/2020/8202584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877393PMC
November 2020