Publications by authors named "Yu Tian"

1,333 Publications

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Electrodeposition of bismuth at a graphene modified carbon electrode and its application as an easily regenerated sensor for the electrochemical determination of the antimicrobial drug metronidazole.

Talanta 2022 Jul 21;251:123758. Epub 2022 Jul 21.

Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland. Electronic address:

Metronidazole is a well-known antimicrobial drug that belongs to the nitroimidazole family of antibiotics. It has been widely used in the treatment of infections, but its accumulation in aquatic environments is an emerging concern. In this study a glassy carbon electrode was modified with graphene (Gr) nanoplatelets and bismuth. Both the Gr and Bi were electrochemically deposited onto the glassy carbon and the modified electrode was employed in the electrochemical detection of metronidazole. At the modified electrode, the reduction of metronidazole was found to be an adsorption-controlled reaction. The optimised sensor, which was fabricated within 6 min, exhibited good selectivity in the presence of various inorganic and organic compounds, good recovery in tap water, and exhibited a linear calibration curve extending from 0.005 to 260 μM, with a limit of detection of 0.9 nM. The sensor was easily regenerated through the simple oxidation of the Bi deposit followed by a 100 s reduction period in the Bi(III) solution to give a newly generated surface. Good reproducibility was achieved using this simple regeneration approach.
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http://dx.doi.org/10.1016/j.talanta.2022.123758DOI Listing
July 2022

CXCL11 Correlates with Immune Infiltration and Impacts Patient Immunotherapy Efficacy: A Pan-Cancer Analysis.

Front Immunol 2022 22;13:951247. Epub 2022 Jul 22.

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.

Background: Immunotherapy has achieved great success in cancer. Nevertheless, many patients cannot benefit from immune checkpoint blockade therapy because of the scantiness of CD8+ T cell infiltration in the tumor microenvironment (TME). CXCL11 is known as a regulator that influences T-cell infiltration into tumors. However, the role of CXCL11 in pan-cancer is still unclear.

Methods: In this study, we investigated the expression and function of CXCL11 across 33 types of cancers based on datasets from The Cancer Genome Atlas (TCGA) database and the Genotype-Tissue Expression (GTEx) database. We analyzed the CXCL11 differential expression in tumor tissue and nontumoral tissue and in different stages of cancers. Moreover, the correlations among CXCL11 expression, prognosis, mismatch repair, tumor mutation burden (TMB), microsatellite instability (MSI), tumor microenvironment, and immune-related genes were evaluated.

Results: CXCL11 expression was significantly higher in tumoral tissue than in nontumoral tissue for most types of cancer. Improved CXCL11 expression was related to an inconsistent prognosis in different cancers. CXCL11 was positively associated with CD8+ T cells and T follicular helper cells in the TME. High expression of CXCL11 was positively related to TMB in BLCA, BRCA, CESC, COAD, LGG, LUAD, OV, SKCM, STAD, THYM, and UCEC. A positive correlation between CXCL11 and MSI was found in COAD and UVM. Moreover, functional analysis of CXCL11 showed that high CXCL11 expression was significantly related to immune-relevant pathways.

Conclusions: CXCL11 might function as a prognostic and immunotherapy marker across cancers. Further investigation into CXCL11 might provide promising insights to improve cancer therapy.
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http://dx.doi.org/10.3389/fimmu.2022.951247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355700PMC
July 2022

A review of literature and meta-analysis of one-puncture success rate in radiofrequency thermocoagulation with different guidance techniques for trigeminal neuralgia.

Eur J Med Res 2022 Aug 6;27(1):141. Epub 2022 Aug 6.

Department of Neurosurgery, The Third Hospital of Jilin University & China-Japan Union Hospital, No. 126, Xiantai Street, Changchun, 130033, China.

Objectives: Radiofrequency thermocoagulation (RFT) is a type of Gasserian ganglion-level ablative intervention that is used for the treatment of trigeminal neuralgia. Guidance technologies are used to assist in the cannulation of the foramen ovale (FO) or foramen rotundum (FR) target. We conducted a systematic review to assess the value of different guidance technologies for RFT.

Methods: We searched PubMed, Embase, the Cochrane database, Web of Science, and PROSPERO for studies published from January 2005 until December 2020. Randomized or nonrandomized comparative studies and nonrandomized studies without internal controls were included. The Cochrane Risk of Bias Tool and the nonrandomized studies of interventions-I tool were used to assess individual study characteristics and overall quality.

Results: Our query identified 765 publications, and we were able to analyze 11 studies on patients suffering from trigeminal neuralgia. Only one study involved randomized controlled trials, whereas the others featured nonrandomized designs, predominantly before-and-after comparisons. Most of them were observational studies. A total of 222 participants were included, with a median number (range) of 20 (3-53) participants. The objective response rate (ORR) of the one-puncture success rate of RFT using puncture guidance for trigeminal neuralgia was 92% [95% CI (0.79-1), P < 0.001]. Statistically significant differences were observed in the cannulation and operation times between the guided and manual puncture groups (P < 0.001).

Conclusions: RFT with puncture guidance technology has an absolute advantage in puncturing the foramen ovale or foramen rotundum.
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http://dx.doi.org/10.1186/s40001-022-00758-0DOI Listing
August 2022

KIR3DL3-HHLA2 and TMIGD2-HHLA2 pathways: The dual role of HHLA2 in immune responses and its potential therapeutic approach for cancer immunotherapy.

J Adv Res 2022 Aug 3. Epub 2022 Aug 3.

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. Electronic address:

Background: T cells and natural killer (NK) cells are essential components of the immune system and are regulated by coinhibitory and costimulatory molecules in which the B7 family and CD28 family play significant roles. Previous immune checkpoint studies on B7/CD28 family members, such as PD-1, have led to remarkable success in cancer immunotherapy. However, there is still a need to find new immune checkpoint molecules. Recent studies have demonstrated that HHLA2 exerts inhibitory and stimulatory functions on the immune system by binding to different receptors on different sites. However, the pathways between HHLA2 and its two receptors on T cells and NK cells remain controversial.

Aim Of Review: Here, we reviewed recent studies about HHLA2 ligand interactions with KIR3DL3 and TMIGD2. We focused on elucidating the pathways between KIR3DL3/TMIGD2 and HHLA2 as well as their function in tumour progression. We also addressed the relationship between HHLA2 expression and the clinical prognosis of cancer patients.

Key Scientific Concepts Of Review: KIR3DL3/TMIGD2-HHLA2 may represent novel pathways within the tumour microenvironment and serve as crucial immune checkpoints for developing novel therapeutic drugs against human cancer.
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http://dx.doi.org/10.1016/j.jare.2022.07.013DOI Listing
August 2022

HHLA2 promotes tumor progression by long non‑coding RNA H19 in human gallbladder cancer.

Int J Oncol 2022 Sep 3;61(3). Epub 2022 Aug 3.

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

Advanced gallbladder cancer (GBC) is one of the most malignant of all types of biliary tract cancers that is associated with poor prognosis and high mortality. Accumulating evidence suggest that the B7 family of proteins serve an essential role in various types of cancers, including GBC. However, the potential function and regulatory mechanism of human endogenous retrovirus‑H long terminal repeat‑associating protein 2 (HHLA2; also known as B7‑H7 or B7H5) in GBC remain poorly understood. In the present study, immunohistochemistry was used to examine the expression pattern of HHLA2 in samples from 89 patients with GBC. The possible association between HHLA2 expression and the clinicopathological parameters, including prognosis, were then assessed. Using lentiviruses, overexpression of HHLA2 plasmid or short‑hairpin RNA (shRNA) of HHLA2 were transfected into GBC‑SD cells to overexpress or knock down HHLA2 expression, respectively. The effects of HHLA2 overexpression and knockdown on the epithelial‑mesenchymal transition (EMT) process on GBC‑SD cells were measured by the western blotting and immunofluorescence staining of collagen I, N‑cadherin, E‑cadherin, vimentin and α‑SMA. By contrast, changes in cell proliferation were measured using EdU assay. Cell invasion and migration were assessed using Transwell and wound‑healing assays, respectively. In addition, a xenograft mouse model was established to evaluate the tumorigenic ability of the GBC cell line after stable transfection with lentivirus for HHLA2 overexpression or shRNA for HHLA2 knockdown. The regulatory relationships among TGF‑β1, long non‑coding RNA (lncRNA) H19 (H19) and HHLA2 were then investigated. The mRNA expression of lncRNA H19 were assessed using reverse transcription‑quantitative PCR, whereas the expression levels of HHLA2 were detected by western blotting and immunofluorescence staining. HHLA2 expression was found to gradually increase as the stages of the GBC samples become more advanced. In addition, the expression level of HHLA2 was calculated to be positively associated with the Nevin stage, American Joint Committee on Cancer stage, tumor invasion and regional lymph node metastasis but was negatively associated with the overall patient survival (OS). experiments demonstrated that overexpression of HHLA2 promoted GBC migration, invasion, proliferation and EMT, whereas experiments found a promoting role of HHLA2 overexpression on GBC tumor growth. After transfection with lentiviruses encoding the overexpression plasmid of lncRNA H19, GBC migration, invasion, proliferation and EMT were increased. By contrast, knocking down HHLA2 expression suppressed TGF‑β1‑ or lncRNA H19 overexpression‑induced GBC migration, invasion, proliferation and EMT. In addition, HHLA2 knockdown significantly reduced the sizes of the GBC tumors . These results suggest that HHLA2 overexpression can promote GBC progression. Conversely, ablation of HHLA2 expression inhibited both TGF‑β1‑ and lncRNA H19‑induced GBC progression, suggesting that HHLA2 is a potential therapeutic target for this disease.
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http://dx.doi.org/10.3892/ijo.2022.5402DOI Listing
September 2022

High-Temperature Superlubricity Realized with Chlorinated-Phenyl and Methyl-Terminated Silicone Oil and Hydrogen-Ion Running-in.

Langmuir 2022 Aug 2. Epub 2022 Aug 2.

State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.

Ceramic friction pairs lubricated with chlorinated-phenyl and methyl-terminated silicone oil (CPSO) systems have potential applications in the aerospace industry. In this study, the effects of the running-in process and temperature on the lubricating performance of CPSO were investigated. The superlubricity of SiN/sapphire lubricated with CPSO was realized at >190 °C after H-ion running-in. The mechanism of this high-temperature superlubricity was investigated by determining the stable adsorption configurations and adsorption energies of CPSO on different surfaces using density functional theory calculations. Compared with that on the SiN surface, the adsorption capacity of CPSO on the hydroxylated SiO surface generated by H-ion running-in increased, whereas the steric hindrance decreased. The viscosity-temperature curve of CPSO was measured, wherein the viscosity and pressure-viscosity coefficient of CPSO considerably decreased with increasing temperature, leading to high-temperature superlubricity in a wide speed/load range. This is the first paper to report oil-based superlubricity at temperatures of 190 °C, or even higher-temperature conditions. Furthermore, it provides guidance for the use of ceramic-CPSO systems in high-temperature conditions, including in the aerospace industry.
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http://dx.doi.org/10.1021/acs.langmuir.2c01523DOI Listing
August 2022

Jujuboside A inhibits oxidative stress damage and enhances immunomodulatory capacity of human umbilical cord mesenchymal stem cells through up-regulating IDO expression.

Chin J Nat Med 2022 Jul;20(7):494-505

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. Electronic address:

Impaired immunomodulatory capacity and oxidative stress are the key factors limiting the effectiveness of mesenchymal stem cell transplantation therapy. The present study was aimed to investigate the effects of jujuboside A (JuA) on the protective effect and immunomodulatory capacity of human umbilical cord mesenchymal stem cells (hUC-MSCs). Hydrogen peroxide was used to establish an oxidative damage model of hUC-MSCs, while PBMCs isolated from rats were used to evaluate the effect of JuA pre-treatment on the immunomodulatory capacity of hUC-MSCs. Furthermore, Hoechst 33258 staining, lactate dehydrogenase test, measurement of malondialdehyde, Western blot, high-performance liquid chromatography; and flow cytometry were performed. Our results indicated that JuA (25 μmol·L) promoted the proliferation of hUC-MSCs, but did not affect the differentiating capability of these cells. JuA pre-treatment inhibited apoptosis, prevented oxidative damage, and up-regulated the protein expression of nuclear factor-erythroid factor 2-related factor 2 and heme oxygenase 1 in hUC-MSCs in which oxidative stress was induced with HO. In addition, JuA pre-treatment enhanced the inhibitory effect of hUC-MSCs against abnormally activated PBMCs, which was related to stimulation of the expression and activity of indoleamine 2,3-dioxygenase. In conclusion, our results demonstrate that JuA pre-treatment can enhance the survival and immunomodulatory ability through pathways related to oxidative stress, providing a new option for the improvement of hUC-MSCs in the clinical setting.
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http://dx.doi.org/10.1016/S1875-5364(22)60176-6DOI Listing
July 2022

The role of valence, arousal, stimulus type, and temporal paradigm in the effect of emotion on time perception: A meta-analysis.

Psychon Bull Rev 2022 Jul 25. Epub 2022 Jul 25.

Cognition and Human Behavior Key Laboratory of Hunan Province, Hunan Normal University, Hunan Province, Changsha, 410011, China.

Anecdotal experiences show that the human perception of time is subjective, and changes with one's emotional state. Over the past 25 years, increasing empirical evidence has demonstrated that emotions distort time perception and usually result in overestimation. Yet, some inconsistencies deserve clarification. Specifically, it remains controversial how valence (positive/negative), arousal (high/low), stimulus type (scenic picture/facial expression/word/sound), and temporal paradigm (reproduction/estimation/discrimination) modulate the effect of emotion on time perception. Thus, the current study aimed to conduct a meta-analysis to quantify evidence for these moderators. After searching the Web of Science, SpiScholar, and Google Scholar, 95 effect sizes from 31 empirical studies were calculated using Hedges'g. The included studies involved 3,776 participants. The results a highlighted significant moderating effect of valence, arousal, stimulus type, and temporal paradigm. Specifically, negative valence tends to result in overestimation relative to positive valence; the increasing arousal leads to increasing temporal dilating; scenic picture, facial picture, and sound are more effective in inducing distortions than word; the overestimation can be better observed by discrimination and estimation paradigms relative to reproduction paradigms, and estimation paradigm is likely to be the most effective. These results suggest that the effect of emotion on time perception is influenced by valence, arousal, stimulus type, and temporal paradigm. These mitigating factors should be considered by scientists when studying time perception.
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http://dx.doi.org/10.3758/s13423-022-02148-3DOI Listing
July 2022

Phloretin enhances autophagy by impairing AKT activation and inducing JNK-Beclin-1 pathway activation.

Exp Mol Pathol 2022 Jul 22;127:104814. Epub 2022 Jul 22.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Erqi District, Zhengzhou 450052, China. Electronic address:

Phloretin is a type of dihydrochalcone that is primarily found in apples and has been reported to possess various potent biological activities, such as anticancer, antioxidant and anti-inflammatory effects. Our previous study has shown that phloretin induces apoptosis in human glioblastoma. In this study, we found that phloretin induced autophagy in SH-SY5Y cells by decreasing p-AKT and p-mTOR levels in the AKT/mTOR pathway and increasing the activation of JNK, the phosphorylation of c-Jun and the expression of Beclin-1. Moreover, the upregulation of Beclin-1 was decreased by SP600125 or a siRNA against c-Jun. Furthermore, SP600125 and siRNAs against c-Jun and Beclin-1 inhibited phloretin-induced autophagy. In addition, inhibition of phloretin-induced autophagy by cotreatment with phloretin and 3-MA decreased phloretin-induced cytotoxicity to SH-SY5Y cells. In conclusion, our results suggest that the AKT/mTOR pathway and JNK-mediated Beclin-1 expression are involved in phloretin-induced autophagy. Phloretin can be used to protect neurons during phloretin treatment of glioblastoma.
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http://dx.doi.org/10.1016/j.yexmp.2022.104814DOI Listing
July 2022

Rapid purification of As(III) in water using iron-manganese composite oxide coupled with sulfite: Importance of the SO radicals.

Water Res 2022 Jul 15;222:118839. Epub 2022 Jul 15.

State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), School of Environment, Harbin Institute of Technology, No.73, Huanghe Road, Nangang, Harbin 150090, China.

Manganese (Mn)-containing composite metal adsorbents are very effective at removing arsenite (As(III)) from contaminated water, however, the low removal speed and oxidation efficiency have limited their further application. In this study, a nonhomogeneous catalytic oxidation-adsorption system was constructed by coupling iron-manganese composite oxide (FeMnO) with sulfite (S(IV)) to enhance the recovery of oxidative capacity and accelerate the removal of As(III). Experimental results showed that the FeMnO/S(IV) system decreased the As(III) concentration from 1079 to <10 µg/L within 10 min and almost completely oxidized As(III) to As(V). In contrast, FeMnO alone removed only 82.4% of As(III) within 30 min, and 60.0% of the adsorbed As(III) was not oxidized. Meanwhile, the adsorption capacity of FeMnO/S(IV) system for As(III) was considerably higher than that of the only-FeMnO system (76.5 > 46.3 mg/g). The efficient and fast As(III) removal was attributed to the SO radical generated by S(IV) acting as the driving force for the redox cycle between As(III) and Mn(II/III/IV). Several environmental factors (e.g., solution pH and inorganic anions) and the reusability and practicality of FeMnO were systematically investigated, and the results further confirmed the superiority of the FeMnO/S(IV) system in As(III) removal. In particular, the proposed FeMnO nanocellulose aerogel effectively purified arsenic-contaminated groundwater using a fixed-bed column. Thus, FeMnO-S(IV) coupling is very promising for the purification of arsenic-contaminated water bodies.
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http://dx.doi.org/10.1016/j.watres.2022.118839DOI Listing
July 2022

Optimization of dry weight assessment in hemodialysis patients via reinforcement learning.

IEEE J Biomed Health Inform 2022 Jul 18;PP. Epub 2022 Jul 18.

Dry weight (DW), defined as the lowest tolerated postdialysis weight following the ultrafiltration (UF) of excess fluid volume, is essential for any dialysis prescription for hemodialysis (HD) patients. However, there is no gold standard for DW assessment, and the difficulty of its accurate assessment increases given individual variations and the dynamic changes caused by the uncertainty of patients' condition. Therefore, the current empirical evaluation process is often crude, imprecise, experience-dependent, and energy-consuming. Here, we highlight the personalized dynamic changes in DW over time rather than the more accurate DW assessments at some point in time and formulate the DW evaluation problem into a sequential decision-making process using the Markov decision process (MDP) framework. A reinforcement learning (RL) algorithm based on a dueling double deep Q-network (Duel-DDQN) is proposed to optimize the DW assessment policy, and a multifaceted inspection is applied to assess policy effectiveness and safety. We utilize ten years of data from the Kidney Disease Center, enrolling 750 HD patients and 243,287 dialysis sessions. Good model calibration is confirmed, and off-policy evaluation demonstrates that our policy outperforms other policies, suggesting a decrease of 7.71% in the expected 5-year mortality rate and of 13.44% in the incidence of intradialytic symptoms compared with those of clinicians' strategy. The RL policy adjusts DW more frequently, responds to DW changes more actively, and observes a larger feature space. It is hoped that the proposed solution will help clinicians assess and monitor DW dynamically, making the estimation process more refined, personalized, and intelligent.
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http://dx.doi.org/10.1109/JBHI.2022.3192021DOI Listing
July 2022

Effect of Plane Mirrors Combined with Au-Nanoparticle Confinement on the Spectral Properties of Fe Plasma Induced by Laser-Induced Breakdown.

ACS Omega 2022 Jul 26;7(27):23605-23610. Epub 2022 Jun 26.

Science and Technology on Electronic Test and Measurement Laboratory, North University of China, Taiyuan, Shanxi 030051, China.

To overcome the shortcomings of low detection sensitivity and high spectral line background noise of traditional laser-induced breakdown spectroscopy (LIBS), a method of combining flat mirrors with gold nanoparticles (Au-NPs) was proposed. First, independent plane mirror and Au-NPs experiments were performed by using aluminum alloy samples. After that, the samples were placed under four conditions (None-LIBS; Three mirrors-LIBS; 20 nm Au-NPs-LIBS; 20 nm Au-NPs and Three mirrors-LIBS), and the differences between various spectral parameters were analyzed. The experimental results show that the optimal number of plane mirrors is 3, and the optimal size of gold nanoparticles is 20 nm. When 20 nm Au-NPs and Three mirrors are used in combination, the plasmonic spectral intensity can be effectively enhanced. The enhancement factor is up to 2.98 (Fe II 240.45 nm), and the signal-to-noise ratio (SNR) is significantly improved up to 10.03. The variation of the plasma temperature between 1 and 5 μs was also investigated, and the experimental results showed that the plasma temperature could be increased by the flat mirror, while the electron temperature was almost unchanged under the action of Au-NPs. It is shown that the combination of the two enhancement methods can effectively increase the spectral intensity and improve the signal-to-noise ratio, which will help to improve the detection performance of the LIBS system.
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http://dx.doi.org/10.1021/acsomega.2c02199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281302PMC
July 2022

Diabetes Mellitus Promotes the Development of Atherosclerosis: The Role of NLRP3.

Front Immunol 2022 29;13:900254. Epub 2022 Jun 29.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Atherosclerosis is one of the main complications of diabetes mellitus, involving a variety of pathogenic factors. Endothelial dysfunction, inflammation, and oxidative stress are hallmarks of diabetes mellitus and atherosclerosis. Although the ability of diabetes to promote atherosclerosis has been demonstrated, a deeper understanding of the underlying biological mechanisms is critical to identifying new targets. NLRP3 plays an important role in both diabetes and atherosclerosis. While the diversity of its activation modes is one of the underlying causes of complex effects in the progression of diabetes and atherosclerosis, it also provides many new insights for targeted interventions in metabolic diseases.
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http://dx.doi.org/10.3389/fimmu.2022.900254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277049PMC
June 2022

Comprehensive detrimental effects of a simulated frequently shifting schedule on diurnal rhythms and vigilance.

Chronobiol Int 2022 09 18;39(9):1285-1296. Epub 2022 Jul 18.

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.

Accumulating data have demonstrated that shift work causes a disturbance in circadian rhythms, which is detrimental to physiology and performance. However, the detailed effects of shift work and especially the underlying mechanisms remain to be further investigated. Frequently shifting schedules are widely used in industries, e.g., maritime tasks, oil mining, and aviation. In this work, we investigated the physiological changes and vigilance of 12 subjects who lived on a 30-day frequent shift working schedule in a confined environment, which mimics the common maritime schedules. Elevated and decreased cortisol levels were observed at different stages during the shift, suggesting the occurrence of stress and fatigue. The results of the Karolinska Sleepiness Scale (KSS) indicate increased sleepiness and a changed pattern of the rhythmicity of sleepiness during the shift. The tests of the Psychomotor Vigilance Task (PVT) reveal that the shift led to a continuously decreasing alertness as the shift working schedule progressed, which is prevalently due to the increasingly slower reaction speed. The PVT time-out errors were significantly increased in the early period but decreased in the late period. In addition, we found recoupling of the correlations between multiple physiological and cognitive variables. For instance, heartbeat rate (HR) and breath rate (BR) showed moderate correlations in the control and early periods but little in the late period. Together, these results reveal substantial alterations in diurnal rhythms, affected vigilance and changed coupling of the correlations of diurnal rhythms, physiology and cognition caused by a shift schedule. Our findings may help in the recognition of the detrimental effects of such working schedules and provide clues for the development of potential mitigations.
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http://dx.doi.org/10.1080/07420528.2022.2101371DOI Listing
September 2022

Single-cell transcriptomic profiling to evaluate the effects of Di(2-ethylhexyl)phthalate exposure on early meiosis of female mouse germ cells.

Chemosphere 2022 Jul 13;307(Pt 1):135698. Epub 2022 Jul 13.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

Di(2-ethylhexyl)phthalate (DEHP) has proven characteristics of an endocrine-disrupting compound (EDC), which can threaten the reproductive health of humans and other animals. In mammals, a series of chromosomal events occur during the meiotic stage of oocytes. External toxins may enter the body and cause infertility and other related diseases. Therefore, it is crucial to explore the influence of DEHP exposure on the molecular mechanism of germ cell meiosis. We used single-cell RNA sequencing (scRNA-seq) to analyse the ovaries of foetal mice at embryonic day 12.5 (E12.5) and E14.5 after maternal DEHP exposure. DEHP exposure further activated the pathways related to DNA repair in germ cells, increased the expression of genes related to DNA damage and changed the developmental trajectory of germ cells. DEHP exposure may affect the proliferation of pregranulosa (PG) cells. Moreover, DEHP exposure altered the signal transduction between PG cells and germ cells. We showed that DEHP affects meiosis by causing DNA damage in oocytes and disrupting the signal transduction between PG cells and germ cells. These results provide a strong theoretical basis for the prevention and treatment of DEHP-mediated female reproductive health problems.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135698DOI Listing
July 2022

Alteration of Skin Microbiome in CKD Patients Is Associated With Pruritus and Renal Function.

Front Cell Infect Microbiol 2022 28;12:923581. Epub 2022 Jun 28.

Department of Urology, Wuxi No. 2 People's Hospital, Affiliated Wuxi Clinical College of Nantong University, Wuxi, China.

Dysbiotic gut microbiome in chronic kidney disease (CKD) patients has been extensively explored in recent years. Skin microbiome plays a crucial role in patients with skin diseases or even systemic disorders. Pruritus is caused by the retention of uremic solutes in the skin. Until now, no studies have investigated the role of skin microbiome in CKD and its association with pruritus. Here, we aim to examine the bacterial profile of skin microbiome in CKD and whether it is correlated to pruritus. A total of 105 CKD patients and 38 healthy controls (HC) were recruited. Skin swab was used to collect skin samples at the antecubital fossa of participants. Bacterial 16S rRNA genes V3-V4 region was sequenced on NovaSeq platform. On the day of skin sample collection, renal function was assessed, and numeric rating scale was used to measure pruritus severity. Principal coordinate analysis (PCoA) revealed a significant difference in bacterial composition between the groups of CKD and HC. A depletion of bacterial diversity was observed in CKD patients. , , , etc. showed significant higher abundance in CKD patients, whereas , , , etc. significantly declined in patients. achieved an acceptable diagnostic biomarker with area under the curve (AUC) value of 0.784 in the receiver operating characteristics (ROC) curve. In addition, CKD patients with pruritus (P-CKD) had a different bacterial community comparing to those without pruritus (non-P-CKD) and HC group. Several bacterial genera showing significant difference between P-CKD and non-P-CKD/HC, such as , significantly declined in P-CKD patients than that in the HC group, and significantly increased in P-CKD patients compared to that in HC subjects. was positively associated with the levels of pruritus severity, blood urea nitrogen (BUN), uric acid, and urine protein; was negatively associated with pruritus severity, whereas it was positively associated with estimated glomerular filtration rate (eGFR) and 24-h urine volume. The dysbiotic of skin microbiome in CKD patients and its association with pruritus and renal function shed a light on skin probiotics.
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http://dx.doi.org/10.3389/fcimb.2022.923581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274276PMC
July 2022

Multiscale unsupervised domain adaptation for automatic pancreas segmentation in CT volumes using adversarial learning.

Med Phys 2022 Jul 14. Epub 2022 Jul 14.

Engineering Research Center of EMR and Intelligent Expert System, Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China.

Purpose: Computer-aided automatic pancreas segmentation is essential for early diagnosis and treatment of pancreatic diseases. However, the annotation of pancreas images requires professional doctors and considerable expenditure. Due to imaging differences among various institution population, scanning devices, imaging protocols, and so on, significant degradation in the performance of model inference results is prone to occur when models trained with domain-specific (usually institution-specific) datasets are directly applied to new (other centers/institutions) domain data. In this paper, we propose a novel unsupervised domain adaptation method based on adversarial learning to address pancreas segmentation challenges with the lack of annotations and domain shift interference.

Methods: A 3D semantic segmentation model with attention module and residual module is designed as the backbone pancreas segmentation model. In both segmentation model and domain adaptation discriminator network, a multiscale progressively weighted structure is introduced to acquire different field of views. Features of labeled data and unlabeled data are fed in pairs into the proposed multiscale discriminator to learn domain-specific characteristics. Then the unlabeled data features with pseudodomain label are fed to the discriminator to acquire domain-ambiguous information. With this adversarial learning strategy, the performance of the segmentation network is enhanced to segment unseen unlabeled data.

Results: Experiments were conducted on two public annotated datasets as source datasets, respectively, and one private dataset as target dataset, where annotations were not used for the training process but only for evaluation. The 3D segmentation model achieves comparative performance with state-of-the-art pancreas segmentation methods on source domain. After implementing our domain adaptation architecture, the average dice similarity coefficient (DSC) of the segmentation model trained on the NIH-TCIA source dataset increases from 58.79% to 72.73% on the local hospital dataset, while the performance of the target domain segmentation model transferred from the medical segmentation decathlon (MSD) source dataset rises from 62.34% to 71.17%.

Conclusions: Correlations of features across data domains are utilized to train the pancreas segmentation model on unlabeled data domain, improving the generalization of the model. Our results demonstrate that the proposed method enables the segmentation model to make meaningful segmentation for unseen data of the training set. In the future, the proposed method has the potential to apply segmentation model trained on public dataset to clinical unannotated CT images from local hospital, effectively assisting radiologists in clinical practice.
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http://dx.doi.org/10.1002/mp.15827DOI Listing
July 2022

Microfluidic Droplet-SERS Platform for Single-Cell Cytokine Analysis via a Cell Surface Bioconjugation Strategy.

Anal Chem 2022 07 11;94(29):10375-10383. Epub 2022 Jul 11.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

A microfluidic-based surface-enhanced Raman scattering (SERS) platform for analyzing cytokines secreted by single cells is reported based on the elaborate bioconjugation of the immuno-sandwich complex on the probed cell surface. This platform integrates the dual functions of microfluidic droplet separation of single cells and SERS measurement. Two immune nanoprobes (capture probe and SERS probe) are introduced into a microfluidic droplet along with a single cell. They were anchored to the cell membrane protein surface by capturing secreted cytokines to form an immune sandwich structure, realizing the enrichment effect of cytokines above the cell membrane surface and the amplification effect of SERS detection probes. This single-cell analytical platform was applied to track specific cell-secreted vascular endothelial growth factor (VEGF) of different cell lines (MCF-7, SGC, and T24), and highly sensitive detection of VEGF was achieved. Chemometric methods (principal component analysis and -distributed stochastic neighbor embedding) were adopted for the SERS data analysis, and the support vector machine (SVM) discriminant model was established to test the data. These chemometric methods successfully identify significant differences in the secreting ability of cytokines among three kinds of cancer cell lines, revealing cell heterogeneity. In addition, the behavior of single cells secreting VEGF was monitored time-dependently and was shown to increase with time. This work demonstrates the importance of tracking specific cells secreting cytokines based on the cell surface bioconjugation strategy. Our developed platform provides guidelines for using the single-cell exocytosis factors as biomarkers to assess the early diagnosis of cancer and provide physiological cues for learning single-cell secretions.
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http://dx.doi.org/10.1021/acs.analchem.2c01249DOI Listing
July 2022

The Counterproductive Effect of Right Anodal/Left Cathodal Transcranial Direct Current Stimulation Over the Dorsolateral Prefrontal Cortex on Impulsivity in Methamphetamine Addicts.

Front Psychiatry 2022 22;13:915440. Epub 2022 Jun 22.

The Affect Cognition and Regulation Laboratory (ACRLab), Institute of Brain and Psychological Sciences, Sichuan Normal University, Chengdu, China.

The current study aimed to evaluate the effect of transcranial direct current stimulation (tDCS) over the dorsolateral prefrontal cortex (DLPFC) on behavioral impulsivity in methamphetamine addicts. Forty-five methamphetamine addicts were recruited and randomly divided into active tDCS and sham tDCS groups to receive a daily tDCS intervention for 5 days, with the intensity set to 2 mA for the active group and 0 mA for the sham group. Anodal and cathodal electrodes were, respectively, placed over the right and left DLPFC. Behavioral impulsivity in methamphetamine addicts was examined by the 2-choice oddball task at 3-time points: before tDCS intervention (baseline), after the first intervention (day 1), and after 5 repeated interventions (day 5). Besides, twenty-four healthy male participants were recruited as the healthy controls who completed a 2-choice oddball task. Analysis of accuracy for the 2-choice oddball task showed that behavioral impulsivity was counterproductively increased in the active group, which was shown by the decreased accuracy for the deviant stimulus. The results suggested that the present protocol may not be optimal and other protocols should be considered for the intervention of methamphetamine addicts in the future.
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http://dx.doi.org/10.3389/fpsyt.2022.915440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257135PMC
June 2022

Understanding the Palatability, Flavor, Starch Functional Properties and Storability of Indica-Japonica Hybrid Rice.

Molecules 2022 Jun 22;27(13). Epub 2022 Jun 22.

Key Laboratory of Agro-Products Processing Ministry of Agriculture, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The rice quality and starch functional properties, as well as the storability of three YY-IJHR cultivars, which included YY12 (biased japonica type YY-IJHR), YY1540 (intermedius type YY-IJHR) and YY15 (biased indica type YY-IJHR), were studied and compared to N84 (conventional japonica rice). The study results suggested that the three YY-IJHR varieties all had greater cooking and eating quality than N84, as they had lower amylose and protein content. The starch of YY-IJHR has a higher pasting viscosity and digestibility, and there was a significant difference among the three YY-IJHR cultivars. Rice aroma components were revealed by GC-IMS, which indicated that the content of alcohols vola-tile components of YY-IJHR were generally lower, whereas the content of some aldehydes and esters were higher than N84. In addition, YY-IJHR cultivars' FFA and MDA contents were lower, which demonstrated that YY-IJHR had a higher palatability and storability than those of N84 in fresh rice and rice stored for 12 months. In conclusion, this study suggested that YY-IJHR had better rice quality and storability than N84. PCA indicated that the grain quality and storability of YY12 and YY15 were similar and performed better than YY1540, while the aroma components and starch functional properties of YY-IJHR cultivars all had significant differences.
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http://dx.doi.org/10.3390/molecules27134009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268750PMC
June 2022

Effects of Storage Temperature on Indica-Japonica Hybrid Rice Metabolites, Analyzed Using Liquid Chromatography and Mass Spectrometry.

Int J Mol Sci 2022 Jul 4;23(13). Epub 2022 Jul 4.

Key Laboratory of Preservation Engineering of Agricultural Products, Ningbo Academy of Agricultural Sciences, Institute of Agricultural Products Processing, Ningbo 315040, China.

The Yongyou series of indica-japonica hybrid rice has excellent production potential and storage performance. However, little is known about the underlying mechanism of its storage resistance. In this study, Yongyou 1540 rice () was stored at different temperatures, and the storability was validated though measuring nutritional components and apparent change. In addition, a broad-targeted metabolomic approach coupled with liquid chromatography-mass spectrometry was applied to analyze the metabolite changes. The study found that under high temperature storage conditions (35 °C), Yongyou 1540 was not significantly worse in terms of fatty acid value, whiteness value, and changes in electron microscope profile. A total of 19 key differential metabolites were screened, and lipid metabolites related to palmitoleic acid were found to affect the aging of rice. At the same time, two substances, guanosine 3',5'-cyclophosphate and pipecolic acid, were beneficial to enhance the resistance of rice under harsh storage conditions, thereby delaying the deterioration of its quality and maintaining its quality. Significant regulation of galactose metabolism, alanine, aspartate and glutamate metabolism, butyrate metabolism, and arginine and proline metabolism pathways were probably responsible for the good storage capacity of Yongyou 1540.
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http://dx.doi.org/10.3390/ijms23137421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266784PMC
July 2022

Homogeneous interfacial water structure favors realizing a low-friction coefficient state.

J Colloid Interface Sci 2022 Jun 28;626:324-333. Epub 2022 Jun 28.

State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.

Hypothesis: The use of water to reduce friction has always played a significant role in a wide range of areas ranging from biology to engineering. Many efforts have been made to extensively investigate the water behavior between two contacted surfaces, but its role in water-based friction remains incompletely understood.

Experiments: Herein, we utilize the sum-frequency generation (SFG) spectroscopy to identify interfacial water structures upon adjusting the wettability of titanium dioxide (TiO) and silicon surfaces. And the corresponding wettability-tunable underwater friction is measured by atomic force microscopy (AFM).

Findings: It demonstrates that enhanced wettability induces higher friction on the TiO surface but lower friction on the silicon surface. Although the tribological properties of TiO show independence of surface forces in contrast to the case of silicon, both TiO and silicon surfaces covered with homogeneous water molecules correspond to a lower friction coefficient. This observation indicates that a homogeneous interfacial water structure, dominating over surface forces, is of the utmost importance for achieving low friction. Our results shed new light on the origins of friction in the presence of water and reveal the ubiquitous role of interfacial water structures on friction.
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http://dx.doi.org/10.1016/j.jcis.2022.06.157DOI Listing
June 2022

Gut Mycobiome in Patients With Chronic Kidney Disease Was Altered and Associated With Immunological Profiles.

Front Immunol 2022 16;13:843695. Epub 2022 Jun 16.

Department of Urology, Affiliated Wuxi No.2 Hospital, Nantong University, Wuxi, China.

Objectives: Mounting evidence suggests that bacterial dysbiosis and immunity disorder are associated with patients with chronic kidney disease (CKD), but the mycobiome is beginning to gain recognition as a fundamental part of our microbiome. We aim to characterize the profile of the mycobiome in the gut of CKD patients and its correlation to serum immunological profiles.

Methods And Materials: Ninety-two CKD patients and sex-age-body mass index (BMI)-matched healthy controls (HCs) were recruited. Fresh samples were collected using sterile containers. ITS transcribed spacer ribosomal RNA gene sequencing was performed on the samples. An immunoturbidimetric test was used to assess the serum levels of immunological features.

Results: The CKD cohort displayed a different microbial community from that in the HC cohort according to principal coordinate analysis (PCoA). (=0.001). The comparison of the two cohorts showed that the CKD cohort had significantly higher gut microbial richness and diversity (<0.05). The CKD cohort had lower abundances of , , , and compared to the HC cohort, while it had higher (<0.05). However, the microbial community alteration was inconsistent with the severity of kidney damage in patients, as only patients in CKD stage 1~3 had differed microbial community concerning for HCs based on PCoA (<0.05). The serum concentration of the kappa light chain in CKD patients was positively associated with , whereas the it was negatively associated with (<0.05).

Conclusions: Not only was gut mycobiome dysbiosis observed in CKD patients, but the dysbiosis was also associated with the immunological disorder. These findings suggest that therapeutic strategies targeting gut mycobiome might be effective.
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http://dx.doi.org/10.3389/fimmu.2022.843695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245424PMC
June 2022

The effect of BMI and age on the outcomes of microsurgical vasoepididymostomy: a retrospective analysis of 181 patients operated by a single surgeon.

Asian J Androl 2022 Jun 24. Epub 2022 Jun 24.

Department of Urology, Peking University Third Hospital, Beijing 100191, China.

To design a treatment plan for patients with epididymal obstruction, we explored the potential impact of factors such as body mass index (BMI) and age on the surgical outcomes of vasoepididymostomy (VE). In this retrospective study, 181 patients diagnosed with obstructive azoospermia (OA) due to epididymal obstruction between September 2014 and September 2017 were reviewed. All patients underwent single-armed microsurgical intussusception VEs with longitudinal two-suture placement performed by a single surgeon (KH) in a single hospital (Peking University Third Hospital, Beijing, China). Six factors that could possibly influence the patency rates were analyzed, including BMI, age, mode of anastomosis, site of anastomosis, and sperm motility and quantity in the intraoperative epididymal fluid. Single-factor outcome analysis was performed via Chi-square test and multivariable analysis was performed using logistic regression. A total of 159 (87.8%, 159/181) patients were followed up. The follow-up time (mean ± standard deviation [s.d.]) was 27.7 ± 9.3 months, ranging from 12 months to 48 months. The overall patency rate was 73.0% (116/159). The multivariable analysis revealed that BMI and age significantly influenced the patency rate (P = 0.008 and 0.028, respectively). Younger age (≤28 years; odds ratio [OR] = 3.531, 95% confidence interval [95% CI]: 1.397-8.924) and lower BMI score (<26.0 kg m; OR = 2.352, 95% CI: 1.095-5.054) appeared to be associated with a higher patency rate. BMI and age were independent factors affecting the outcomes of microsurgical VEs depending on surgical expertise and the use of advanced technology.
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http://dx.doi.org/10.4103/aja202238DOI Listing
June 2022

Transcription factor Sp9 is a negative regulator of D1-type MSN development.

Cell Death Discov 2022 Jun 30;8(1):301. Epub 2022 Jun 30.

Institute of Pediatrics, Children's Hospital of Fudan University, state Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, 200032, Shanghai, China.

The striatum is the main input structure of the basal ganglia, receiving information from the cortex and the thalamus and consisting of D1- and D2- medium spiny neurons (MSNs). D1-MSNs and D2-MSNs are essential for motor control and cognitive behaviors and have implications in Parkinson's Disease. In the present study, we demonstrated that Sp9-positive progenitors produced both D1-MSNs and D2-MSNs and that Sp9 expression was rapidly downregulated in postmitotic D1-MSNs. Furthermore, we found that sustained Sp9 expression in lateral ganglionic eminence (LGE) progenitor cells and their descendants led to promoting D2-MSN identity and repressing D1-MSN identity during striatal development. As a result, sustained Sp9 expression resulted in an imbalance between D1-MSNs and D2-MSNs in the mouse striatum. In addition, the fate-changed D2-like MSNs survived normally in adulthood. Taken together, our findings supported that Sp9 was sufficient to promote D2-MSN identity and repress D1-MSN identity, and Sp9 was a negative regulator of D1-MSN fate.
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http://dx.doi.org/10.1038/s41420-022-01088-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247084PMC
June 2022

Normalization of tumor vasculature: A potential strategy to increase the efficiency of immune checkpoint blockades in cancers.

Int Immunopharmacol 2022 Jun 25;110:108968. Epub 2022 Jun 25.

Department of General Surgery, Shengjing Hospital of China Medical University, No.36.Sanhao stress, Shenyang 110004, Liaoning Province, China. Electronic address:

Immune checkpoint inhibitors (ICIs) eliminate tumor cells by reactivating CD8 + T cells using the cytotoxic effects of the immune system. However, in this process, tumor angiogenic factors and abnormal formation of tumor blood vessels are not conducive to the treatment of ICIs. In the tumor microenvironment (TME), proangiogenic factors prevent dendritic cell maturation, reduce T cell infiltration, and recruit inhibitory immune cells such as regulatory T (Treg) cells. Abnormal tumor blood vessels also prevent immune cells and chemotherapy drugs from reaching the target effectively and lead to poor perfusion and severe hypoxia of the tumor. Treatment with antiangiogenic inhibitors can block the transmission of abnormal angiogenesis signals and promote the normalization of tumor vasculature. Therefore, the combination of antiangiogenic inhibitors and ICIs is used in clinical therapy. Combination therapy has been proven theoretically feasible in preclinical trials, and many clinical trials have been completed to confirm its safety and efficacy.
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http://dx.doi.org/10.1016/j.intimp.2022.108968DOI Listing
June 2022

Portable and on-site electrochemical sensor based on surface molecularly imprinted magnetic covalent organic framework for the rapid detection of tetracycline in food.

Food Chem 2022 Nov 20;395:133532. Epub 2022 Jun 20.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:

In this study, for the first time, surface molecularly imprinted magnetic covalent organic frameworks ([email protected]@MIPs) were combined with disposable screen-printed electrode (SPE) to construct a portable and on-site electrochemical sensor for the rapid detection of tetracycline (TC). The [email protected]@MIPs, which was prepared by layer-by-layer modification method, had good magnetism and excellent adsorption ability. With the help of disposable SPE, equipped with a magnet, the electrode modification process was simplified and the detection efficiency was improved. Under optimal conditions, the fabricated electrochemical sensor exhibited linearity ranging from 1 × 10 to 1 × 10 g mL. It had good selectivity, excellent reproducibility, desirable stability and remarkable applicability. The fabricated sensor was successfully applied to detect TC in real samples with satisfactory recoveries (96.15-106.20%). The detection strategy separated the recognition and adsorption process from the electrochemical detection process, providing a design idea for the application of COFs in the construction of high-efficiency molecularly imprinted electrochemical sensors.
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http://dx.doi.org/10.1016/j.foodchem.2022.133532DOI Listing
November 2022

Regulatory Effect of Mung Bean Peptide on Prediabetic Mice Induced by High-Fat Diet.

Front Nutr 2022 9;9:913016. Epub 2022 Jun 9.

College of Food, Heilongjiang Bayi Agricultural University, Daqing, China.

Dietary supplementation with mung bean peptides (MBPs) has several health benefits. However, the effect of MBPs on prediabetes and gut microbiota imbalance caused by a high-fat diet (HFD) has not been thoroughly studied. In this study, dietary supplementation with MBPs for 5 weeks significantly reduced HFD-induced body weight gain, hyperglycaemia, hyperlipidaemia, insulin resistance, inflammation, and oxidative stress and alleviated liver and kidney damage in mice. In addition, it significantly reversed the HFD-induced gut microbiota imbalance, increased the gut microbial diversity, and decreased the abundance of Firmicutes and Bacteroidetes in prediabetic mice. Furthermore, we identified and as important eubacteria with the potential to alleviate the clinical symptoms of prediabetes. According to PICRUSt2 analysis, the changes in intestinal microflora induced by MBPs diet intervention may be related to the downregulation of expression of genes such as , , and and regulation of seven pathways, including pyruvate, succinic acid, and butyric acid. Moreover, 17 genera with significantly altered levels in the intestine of HFD-fed mice, including , , and , were significantly correlated with 26 important differential metabolites, such as D-glutathione, anti-oleic acid, and cucurbitacin. Overall, these results show that MBPs diet intervention plays a key role in the management of HFD-induced prediabetes.
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http://dx.doi.org/10.3389/fnut.2022.913016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218720PMC
June 2022

Potential roles of serum ATPase and AMPase in predicting diagnosis of colorectal cancer patients.

Bioengineered 2022 06;13(6):14204-14214

Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Colorectal cancer (CRC) is a common gastrointestinal cancer with high incidence and mortality rates. CRC may be associated with regulation of circulating nucleotides. This study aimed to evaluate the serum levels of nucleotide-metabolizing enzymes (ATPase and AMPase) in patients with CRC and to explore the clinical diagnostic value of these enzymes. The gene set variation analysis (GSVA) score of the ATP-adenosine signature was calculated using tumor samples from The Cancer Genome Atlas (TCGA). ATP-adenosine signaling plays a central role in CRC progression. A total of 135 subjects, including 87 patients with CRC and 48 healthy controls, were included. The serum levels of ATPase and AMPase in the CRC group were significantly higher than those in the control group ( < 0.05). Furthermore, ATP and AMP hydrolysis levels significantly increased in the advanced CRC group ( < 0.05). ATP and AMP hydrolysis was decreased by the ENTPDase inhibitors (POM-1 and ARL67156) and CD73 inhibitor (APCP). The sensitivities of ATPase and AMPase were 95.4% and 75.9%, respectively, which were higher than those of CEA (67.8%) and CA19-9 (72.4%). The specificities of ATPase and AMPase were 69.9% and 73.9%, respectively, which were higher than that of CA19-9 (47.8%). The combination of CEA, ATPase, and AMPase demonstrated high sensitivity (92.0%) and specificity (87.0%). Collectively, ATPase and AMPase activities are upregulated in CRC with considerable diagnostic significance. The combination of CEA, ATPase, and AMPase may provide a novel approach for CRC screening.
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http://dx.doi.org/10.1080/21655979.2022.2084423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342199PMC
June 2022

The Underlying Role of the Glymphatic System and Meningeal Lymphatic Vessels in Cerebral Small Vessel Disease.

Biomolecules 2022 05 25;12(6). Epub 2022 May 25.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

There is a growing prevalence of vascular cognitive impairment (VCI) worldwide, and most research has suggested that cerebral small vessel disease (CSVD) is the main contributor to VCI. Several potential physiopathologic mechanisms have been proven to be involved in the process of CSVD, such as blood-brain barrier damage, small vessels stiffening, venous collagenosis, cerebral blood flow reduction, white matter rarefaction, chronic ischaemia, neuroinflammation, myelin damage, and subsequent neurodegeneration. However, there still is a limited overall understanding of the sequence and the relative importance of these mechanisms. The glymphatic system (GS) and meningeal lymphatic vessels (mLVs) are the analogs of the lymphatic system in the central nervous system (CNS). As such, these systems play critical roles in regulating cerebrospinal fluid (CSF) and interstitial fluid (ISF) transport, waste clearance, and, potentially, neuroinflammation. Accumulating evidence has suggested that the glymphatic and meningeal lymphatic vessels played vital roles in animal models of CSVD and patients with CSVD. Given the complexity of CSVD, it was significant to understand the underlying interaction between glymphatic and meningeal lymphatic transport with CSVD. Here, we provide a novel framework based on new advances in main four aspects, including vascular risk factors, potential mechanisms, clinical subtypes, and cognition, which aims to explain how the glymphatic system and meningeal lymphatic vessels contribute to the progression of CSVD and proposes a comprehensive insight into the novel therapeutic strategy of CSVD.
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http://dx.doi.org/10.3390/biom12060748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221030PMC
May 2022
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