Publications by authors named "Yu Ping Liu"

80 Publications

Gender-specific and age-specific associations of the homoeostasis model assessment for IR (HOMA-IR) with albuminuria and renal function impairment: a retrospective cross-sectional study in Southeast China.

BMJ Open 2021 Dec 17;11(12):e053649. Epub 2021 Dec 17.

Department of Family Medicine, Chang Gung Memorial Hospital Linkou Main Branch, Taoyuan, Taiwan

Objectives: The study aimed to investigate the association of insulin resistance (IR), which was estimated by the homoeostasis model assessment for IR (HOMA-IR), with albuminuria and renal function impairment in a general Chinese population.

Design: A retrospective cross-sectional study.

Setting And Participants: A total of 13 742 adults (age: ≥18 years) who underwent a health check-up at a hospital in Southeast China during 2013-2014 were enrolled. 216 subjects were excluded due to lack of enough fasting time, be pregnant, have chronic diseases influencing metabolic functions or have glomerulonephritis, renal cancer, kidney transplant. Eventually, 7552 men and 5974 women were included for the present analysis.

Primary Outcome Measures: The association of HOMA-IR with albuminuria and renal function impairment were analysed. The HOMA-IR cut-off value for detecting albuminuria and renal function impairment were determined.

Results: An increase in the HOMA-IR quartile was significantly associated with the prevalence of albuminuria and renal function impairment in all men and women aged >45 years. The multivariable logistic regression analyses revealed a significant association of the HOMA-IR with albuminuria and renal function impairment in subjects aged >45 years of the fourth quartiles compared with those of the first quartile after adjusting for potential confounders (albuminuria: men OR, 2.39; 95% CI 1.51 to 3.79, p<0.001; women OR, 2.40; 95% CI 1.44 to 4.01; p0.001; renal function impairment: men OR, 2.30; 95% CI 1.50 to 3.51; p<0.001; women OR, 2.20; 95% CI 1.35 to 3.58; p=0.002). The optimal cut-off value of HOMA-IR for detecting albuminuria and renal function impairment was 2.69 in men aged ≤45 years, 1.60 in men aged >45 years and 1.86 in women aged >45 years.

Conclusions: Our study revealed that HOMA-IR was significantly associated with albuminuria and renal function impairment in individuals aged >45 years.
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http://dx.doi.org/10.1136/bmjopen-2021-053649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685940PMC
December 2021

Association between infection and food-specific immunoglobulin G in Southwest China.

World J Clin Cases 2021 Nov;9(32):9815-9824

Health Management Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan Province, China.

Background: () has been found to be associated with extragastrointestinal diseases, possibly including adverse food reactions (such as food allergy or intolerance). However, there are few studies on and food allergy or intolerance, and the results are inconsistent. Food-specific immunoglobulin (Ig) G has been revealed to be associated with food allergy or intolerance and can be used as a marker to explore the correlation between infection and food allergy or intolerance.

Aim: To explore the relationship between infection and food-specific IgG.

Methods: We retrospectively analyzed the physical examination data of 21822 subjects from February 2014 to December 2018 in this study. infection was detected using the C urea breath test. Food-specific IgG of eggs, milk and wheat in serum was assessed. Subjects were grouped according to positivity, and the positive rates of three kinds of food-specific IgG were compared between the two groups. Multivariable logistic regression analysis was performed to elucidate the association between infection and food-specific IgG.

Results: The total infection rate of was 39.3%, and the total food-specific IgG-positive rates of eggs, milk and wheat were 25.2%, 9.0% and 4.9%, respectively. The infection rate of was higher in males than in females, while the positive rates of food-specific IgG were lower in males than in females. The positive rates of food-specific IgG decreased with age in both males and females. In the -positive groups, the positive rates of food-specific IgG of eggs, milk and wheat were all lower than those in the -negative groups. Multivariate logistic regression analysis revealed that infection was negatively correlated with the food-specific IgG-positive rates of eggs, milk and wheat (odds ratio value of eggs 0.844-0.873, milk 0.741-0.751 and wheat 0.755-0.788, in different models).

Conclusion: infection was found to be negatively associated with the food-specific IgG of eggs, milk and wheat in Southwest China.
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http://dx.doi.org/10.12998/wjcc.v9.i32.9815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610918PMC
November 2021

Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae).

J Plant Physiol 2022 Jan 20;268:153575. Epub 2021 Nov 20.

School of Life Sciences, Qinghai Normal University, Xining, 810008, China.

Psammochloa villosa is a desert plant growing in Northwest China with considerable resistance to abiotic stress, including drought, cold, and salt. To facilitate future studies of stress resistance in Psammochloa villosa, we sought to establish a suite of reference (or housekeeping) genes for utilization within future gene expression studies. Specifically, we selected nine candidate genes based on prior studies and new transcriptomic data for P. villosa, and we evaluated their expression stability in three different tissues of P. villosa under different treatments simulating abiotic stress conditions using four different bioinformatics assessments. Our results showed that TIP41 (TIP41-like family protein) was the most stable reference gene in drought- and salt-stressed leaves and salt-stressed stems, ELF-1α (elongation factor 1-α) was the most stable in cold-stressed leaves and drought- and salt-stressed roots, ACT (actin) was the most stable in drought-stressed stems, TUA (α-tubulin) was the most stable in cold-stressed stems, and 18S rRNA (18S ribosomal RNA) was the most stable in cold-stressed roots. Additionally, we tested the utility of these candidate reference genes to detect the expression pattern of P5CS (Δ-pyrroline-5-carboxylate synthetase), which is a drought-related gene. This study is the first report on selecting and validating reference genes of P. villosa under various stress conditions and will benefit future investigations of the genomic mechanisms of stress resistance in this ecologically important species.
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http://dx.doi.org/10.1016/j.jplph.2021.153575DOI Listing
January 2022

Problem-solving deficits in methcathinone use disorder.

Psychopharmacology (Berl) 2021 Sep 22;238(9):2515-2524. Epub 2021 Jul 22.

Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Wanping South Road 600, Xuhui, Shanghai, China.

Rationale: The use of methcathinone (MCAT), a psychostimulant drug that can lead to long-term health risks and executive dysfunction, increased to an alarming rate in recent years. Impairments in low-level executive function have been reported in substance use disorder. However, little empirical evidence is available regarding high-level executive function (e.g., problem solving), which may act as a risk factor for relapse.

Objectives: The present study aimed to investigate whether the problem-solving ability was altered in abstinent individuals with methcathinone use disorder (MCUD). Here, we tested fifty male MCUD individuals (short-term MCUD group: twenty-nine patients with MCAT use less than 3 years, long-term MCUD group: twenty-one patients with MCAT use longer than 3 years, which were split by medium years of drug use) and twenty-four well-matched healthy controls (HC) in the Tower of Hanoi task (TOH) to assess the impact of task difficulty on drug-related changes in problem-solving performance. We used several measures to characterize problem-solving performance: the number of mistakes made, the completion time of the task, and the thinking time before the first move.

Results: In the low task difficulty condition, the MCUD group and HC group showed similar levels of mistakes and completion time, while in the high task difficulty condition, the MCUD group reported more mistakes (the mean number of mistakes in each trial: 1.41 ± 1.15 vs 0.79 ± 0.76, P = 0.019, Cohen's d = 0.635) and longer completion time in the task (the mean completion time in each trial: 45.83 ± 20.51 s vs 33.40 ± 15.10 s, P = 0.010, Cohen's d = 0.690) than the HC group. The thinking time before the first move did not differ significantly between groups (P = 0.257). We further found that the long-term (more than 3 years) MCUD group made more mistakes than the short-term MCUD group and HC group, mainly in the highly difficult subtasks. The longer time than HCs was reported in the long-term MCUD group among high task difficulty of subtasks. In addition, there was a positive correlation between years of MCAT use and the number of mistakes made in high task difficulty TOH task (r = 0.326, P = 0.021).

Conclusions: Chronic methcathinone use was associated with deficits in problem-solving performance, which depended on the degree of task difficulty. The impairment was more evident in the long-term (> 3 years) MCAT group.
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http://dx.doi.org/10.1007/s00213-021-05874-zDOI Listing
September 2021

The Association of Gut Microbiota With Osteoporosis Is Mediated by Amino Acid Metabolism: Multiomics in a Large Cohort.

J Clin Endocrinol Metab 2021 09;106(10):e3852-e3864

Guangdong Provincial Key Laboratory of Food, Nutrition and Health; Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Context: Several small studies have suggested that the gut microbiome might influence osteoporosis, but there is little evidence from human metabolomics studies to explain this association.

Objective: This study examined the association of gut microbiome dysbiosis with osteoporosis and explored the potential pathways through which this association occurs using fecal and serum metabolomics.

Methods: We analyzed the composition of the gut microbiota by 16S rRNA profiling and bone mineral density using dual-energy X-ray absorptiometry in 1776 community-based adults. Targeted metabolomics in feces (15 categories) and serum (12 categories) were further analyzed in 971 participants using ultra-high-performance liquid chromatography coupled to tandem mass spectrometry.

Results: This study showed that osteoporosis was related to the beta diversity, taxonomy, and functional composition of the gut microbiota. The relative abundance of Actinobacillus, Blautia, Oscillospira, Bacteroides, and Phascolarctobacterium was positively associated with osteoporosis. However, Veillonellaceae other, Collinsella, and Ruminococcaceae other were inversely associated with the presence of osteoporosis. The association between microbiota biomarkers and osteoporosis was related to levels of peptidases and transcription machinery in microbial function. Fecal and serum metabolomics analyses suggested that tyrosine and tryptophan metabolism and valine, leucine, and isoleucine degradation were significantly linked to the identified microbiota biomarkers and to osteoporosis, respectively.

Conclusion: This large population-based study provided robust evidence connecting gut dysbiosis, fecal metabolomics, and serum metabolomics with osteoporosis. Our results suggest that gut dysbiosis and amino acid metabolism could be targets for intervention in osteoporosis.
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http://dx.doi.org/10.1210/clinem/dgab492DOI Listing
September 2021

Identifying the Dominant Role of Pyridinic-N-Mo Bonding in Synergistic Electrocatalysis for Ambient Nitrogen Reduction.

ACS Nano 2021 Jun 21. Epub 2021 Jun 21.

National Institute for Advanced Materials, School of Materials Science and Engineering, Nankai University, Tianjin 300350, China.

For electrochemical nitrogen reduction reaction (NRR), hybridizing transition metal (TM) compounds with nitrogen-doped carbonaceous materials has been recognized as a promising strategy to improve the activity and stability of electrocatalysts due to the synergistic interaction from the TM-N-C active sites. Nevertheless, up to date, the fundamental mechanism of this so-called synergistic electrocatalysis for NRR is still unclear. Particularly, it remains ambiguous which configuration of N dopants, either pyridinic N or pyrrolic N, when coordinated with the TM, predominately contributes to this synergy. Herein, a self-assembled three-dimensional 1T-phase MoS microsphere coupled with N-doped carbon was developed (termed MoS/NC), showing an impressive NRR performance in neutral medium. The hybridization of MoS and N-doped carbon can synergistically enhance the NRR efficiency by optimizing the electron transfer of catalyst. Acidification/blocking/poisoning experiments reveal the decisive role of pyridinic-N-Mo bonding, rather than pyrrolic-N-Mo bonding, in synergistically enhancing NRR electrocatalysis. The electrochemical-based Fourier transform infrared spectroscopy ( FTIR) technology provides deep insights into the substantial contribution of pyridinic-N-MoS sites to NRR electrocatalysis and further uncover the underlying mechanism (associative pathway) at a molecular level.
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http://dx.doi.org/10.1021/acsnano.1c03465DOI Listing
June 2021

Association of MTHFR C677T polymorphism with primary open angle glaucoma: a Meta-analysis based on 18 case-control studies.

Int J Ophthalmol 2021 18;14(6):896-902. Epub 2021 Jun 18.

Health Management Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China.

Aim: To systematically understand the genetic association between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and primary open angle glaucoma (POAG).

Methods: A comprehensive literature search in Google Scholar, PubMed, Science Citation Index, Foreign Medical Literature Retrieval Service, Chinese National Knowledge Infrastructure and Wanfang Databases was performed to collect all eligible studies up to August 2019. Study selection, data abstraction and study quality evaluation were performed by two independent investigators. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association.

Results: Eighteen case-control studies including 2156 cases and 2201 controls were identified. There was no significant difference in the terms of MTHFR C677T polymorphism and POAG in the Caucasian population (for T C OR=1.11, 95%CI: 0.88 to 1.39; for TT CC OR=1.01, 95%CI: 0.76 to 1.36; for TT+TC CC OR=1.15, 95%CI: 0.84 to 1.58 and for TT TC+CC OR=1.02, 95%CI: 0.78 to 1.33). However, a significant effect was revealed in the Asian population (for T C OR=1.34, 95%CI: 1.12 to 1.59; for TT+TC CC OR=1.41, 95%CI: 1.14 to 1.76).

Conclusion: Based on 18 eligible studies, we provide a correlation between MTHFR C677T polymorphism and POAG among the Asians subgroup indicating that the T allele or TT +TC genotype may play a critical role in POAG development in Asians.
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http://dx.doi.org/10.18240/ijo.2021.06.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165629PMC
June 2021

Isoflavone biomarkers are inversely associated with atherosclerosis progression in adults: a prospective study.

Am J Clin Nutr 2021 07;114(1):203-213

Department of Epidemiology, Guangdong Provincial Key Laboratory of Food, Nutrition, and Health, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Background: Many studies have examined associations between dietary isoflavones and atherosclerosis, but few used objective biomarkers.

Objectives: We examined the associations of isoflavone biomarkers (primary analyses) and equol production (secondary analyses) with the progression of carotid intima-media thickness (cIMT), and whether inflammation, systolic blood pressure (SBP), blood lipids, and sex hormone-binding globulin (SHBG) mediated these associations, in Chinese adults.

Methods: This 8.8-y prospective study included 2572 subjects (40-75 y old) from the GNHS (Guangzhou Nutrition and Health Study; 2008-2019). The concentrations of daidzein, genistein, and equol were assayed by an HPLC-tandem MS in serum (n = 2572) at baseline and in urine (n = 2220) at 3-y intervals. The cIMT of the common carotid artery (CCA) and bifurcation segment were measured by B-mode ultrasound every 3 y, and the progressions of cIMT ( ∆cIMT) were estimated using the regression method.

Results: Multivariable linear mixed-effects models (LMEMs) and ANCOVA revealed that subjects with higher serum isoflavones tended to have lower increases of CCA-cIMT. The mean ± SEM differences in 8.8-y ∆CCA-cIMT between extreme tertiles of serum isoflavones were -17.1 ± 8.4, -20.6 ± 8.3, and -23.3 ± 10.4 μm for daidzein, total isoflavone, and equol (P-trends < 0.05), respectively. LMEMs showed that the estimated yearly changes (95% CIs) (μm/y) in CCA-IMT were -2.0 (-3.8, -0.3), -1.9 (-3.6, -0.1), and -2.1 (-3.8, -0.3) in the highest (compared with the lowest) tertile of daidzein, genistein, and total isoflavones, respectively (P-interaction < 0.05). Path analyses indicated that the serum equol-atherosclerosis association was mediated by increased SHBG and decreased SBP. Similar beneficial associations were observed in the secondary analyses.

Conclusions: Serum isoflavones and equol exposure were associated with reduced cIMT progression, mediated by SHBG and SBP.
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http://dx.doi.org/10.1093/ajcn/nqab008DOI Listing
July 2021

Circulating Very-Long-Chain Saturated Fatty Acids Were Inversely Associated with Cardiovascular Health: A Prospective Cohort Study and Meta-Analysis.

Nutrients 2020 Sep 4;12(9). Epub 2020 Sep 4.

Department of Epidemiology, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.

Saturated fatty acids with different chain lengths have different biological activities, but little is known about very-long-chain saturated fatty acids (VLCSFAs). This study investigated the associations between the circulating VLCSFAs and cardiovascular health. This community-based cohort study included 2198 adults without carotid artery plaques (CAPs) at baseline. The percentage of baseline erythrocyte VLCSFA (arachidic acid (C20:0), behenic acid (C22:0), and lignoceric acid (C24:0)) was measured by gas chromatography. The presence of CAPs was determined at baseline and every 3 years thereafter by ultrasound examination. A meta-analysis was conducted to summarize the pooled associations between circulating VLCSFAs and the risk of cardiovascular diseases (CVDs). During a median of 7.2 years of follow-up, 573 women (35.1%) and 281 men (49.6%) were identified as CAP incident cases. VLCSFAs were inversely related with CAP risk in women (all -trend <0.05) but not in men. Multivariate adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of CAPs for the highest (vs. lowest) quartile were 0.80 (0.63-1.01) for C20:0, 0.71 (0.56-0.89) for C22:0, 0.75 (0.59-0.94) for C24:0, and 0.69 (0.55-0.87) for total VLCSFAs in women. The pooled HRs (95% CIs) of CVDs for the highest (vs. lowest) circulating VLCSFAs from seven studies including 8592 participants and 3172 CVD events were 0.67 (0.57-0.79) for C20:0, 0.66 (0.48-0.90) for C22:0, and 0.57 (0.42-0.79) for C24:0, respectively. Our findings suggested that circulating VLCSFAs were inversely associated with cardiovascular health.
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http://dx.doi.org/10.3390/nu12092709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551797PMC
September 2020

[Comparative transcriptome and proteome profiling of chlorophyll metabolism pathway in four types of Magnolia officinalis].

Zhongguo Zhong Yao Za Zhi 2020 Aug;45(16):3826-3836

Pharmacy College, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources Chengdu 611137, China.

Magnolia officinalis is a traditional Chinese medicine,with many years of cultivating process, M. officinalis leaves show more differentiation types due to the exchange of seeds from different provenances. "Da Ao"(DA), "Xiao Ao"(XA), "Chuan Hou"(CH),and "Liu Ye"(LY)are the main types of M. officinalis in Sichuan province of China,and there were obvious differences in growth rate,chemical composition,leaf shape and leaf colour. This study selected different types of M. officinalis leaves(DA,XA,LY and CH)from Sichuan to determine their chlorophyll content. Transcriptomic level sequencing of different types of M. officinalis leaf tissues was by high-throughput sequencing analysis and proteomics used an integrated approach involving TMT labelling and LC-MS/MS to quantify the dynamic changes of the whole proteome of M. officinalis. The results showed that CH had the lowest chlorophyll content while DA had the highest chlorophyll content. Furthermore,transcriptome and proteomics results showed that chlorophyll synthesis pathway in DA glutamine-tRNA reductase,urinary porphyrins decarboxylase(UROD),oxygen-dependent protoporphyrin(ODCO),the original-Ⅲ oxidase protoporphyrin oxidase(PPO),magnesium chelating enzyme subunit ChlD,protoporphyrin magnesium Ⅸ monomethyl ester [oxidative] cyclase(MPPMC)were significantly higher than CH,XA and LY,consistent in the results of determination of chlorophyll content(chlorophyll content was highest of 37.56 mg·g~(-1) FW). Some rate-limiting enzymes related to the chlorophyll synthesis,such as ODCO,PPO and MPPMC were tested by Parallel Reaction Monitoring(PRM),and the results showed that the rate-limiting enzyme content in DA was higher than that in other three types. Therefore,based on the differences in leaf color of four types of M. officinalis,the research conducted a preliminary study on the chlorophyll metabolism pathway in leaves of different types of M. officinalis,and explored relevant genes and proteins causing leaf color differences from the molecular level,so as to lay a foundation for studying the differences in growth and development of different types of M. officinalis.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200527.104DOI Listing
August 2020

Long-term characteristics of criteria air pollutants in megacities of Harbin-Changchun megalopolis, Northeast China: Spatiotemporal variations, source analysis, and meteorological effects.

Environ Pollut 2020 Dec 19;267:115441. Epub 2020 Aug 19.

State Key Laboratory of Urban Water Resource and Environment, Harbin, 150090, China; School of Environment, Harbin Institute of Technology, Harbin, 150090, China. Electronic address:

The hourly concentration of six criteria air pollutants in the Harbin-Changchun region were used to investigate the status and spatiotemporal variation of target air pollutants and their relationships with meteorological factors. The annual concentrations of particulate matters during 2013-2017 were two times higher than the Chinese Ambient Air Quality Standards (CAAQS) Grade Ⅱ. The annual O concentration increased by two times during 2013-2018 in Harbin. The concentration of PM, SO, NO, and CO depicted a similar seasonal trend with an order of winter > autumn > spring > summer. The consistent interannual variation trends of PM/CO, NO and SO indicated that the formation of secondary inorganic aerosols in the annual scale was dominated by the concentrations of NO and SO. The interannual variations of the individual meteorological factors causing on PM and O during 2013-2018 varied significantly in seasonal scale. The interannual variations were stable in annual scale indicating that the continuous decline of PM during 2014-2018 can be attributed to the comprehensive and strict prohibition of small coal-fired boilers and straw burning in the study area. Meanwhile, the increase in O during 2013-2018 in the study area were mainly attributed to the rapid growth of the emission of its precursor (VOCs and NOx). The influence of meteorology on PM and ozone were the most stable and strongest in winter than that in the other three seasons.
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http://dx.doi.org/10.1016/j.envpol.2020.115441DOI Listing
December 2020

Higher healthy lifestyle scores are associated with greater bone mineral density in middle-aged and elderly Chinese adults.

Arch Osteoporos 2020 08 17;15(1):129. Epub 2020 Aug 17.

Department of Epidemiology, School of Public Health, Sun Yat-sen University, No. 74 Zhongshan Road 2, Guangzhou, 510080, People's Republic of China.

This study examined the association between healthy lifestyle score (HLS), which contained 7 items (smoking, BMI, physical activity, diet, alcohol, sleep and anxiety) and BMD. Results showed HLS was positively associated with BMD at all studied sites, suggesting that healthier lifestyle patterns might be beneficial to bone health.

Purpose: Previous studies have reported favourable associations of individual healthy lifestyle factors with bone mineral density (BMD), but limited evidence showed the relationship of a combined healthy lifestyle score (HLS) with BMD. This study examined the association between the HLS and BMD.

Methods: This community-based cross-sectional study included 3051 participants aged 40-75 years. The HLS contained 7 items (smoking, BMI, physical activity, diet quality, alcohol intake, sleep and anxiety). BMD values of whole body (WB), lumbar spine 1-4 (L), total hip (TH) and femur neck (FN) were measured using dual-energy X-ray absorptiometry.

Results: After adjusting for potential covariates, HLS was positively associated with BMD at all studied sites (P-trend < 0.01). The mean BMDs were 2.69% (WB), 5.62% (L), 6.13% (TH) and 5.71% (FN) higher in participants with HLS of 6-7 points than in those with HLS of 0-2 points. The per 1 of 7 unit increase in the HLS was associated with increases of 7.63 (WB)-13.4 (TH) mg/cm BMD levels at all sites. These favourable associations tended to be more pronounced in men than in women. Among the 7 items, physical activity contributed most to the favourable associations, followed by BMI, non-smoking and diet; the other three items played little roles. Sensitivity analyses showed that the significant associations remained after excluding any one of the 7 components or excluding fracture subjects at all sites.

Conclusion: Higher HLS was associated with greater BMD in middle-aged and elderly Chinese, suggesting that healthier lifestyle patterns might be beneficial to bone health.
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http://dx.doi.org/10.1007/s11657-020-00758-9DOI Listing
August 2020

Higher flavonoid intake is associated with a lower progression risk of non-alcoholic fatty liver disease in adults: a prospective study.

Br J Nutr 2021 02 27;125(4):460-470. Epub 2020 Jul 27.

Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou510080, Guangdong, People's Republic of China.

Flavonoid-rich foods have shown a beneficial effect against non-alcoholic fatty liver disease (NAFLD) in short-term randomised trials. It is uncertain whether the usual dietary intake of flavonoids may benefit patients with NAFLD. The present study evaluated the association between the usual intake of flavonoids and the risk of progression in NAFLD. The prospective study included 2694 adults from the Guangzhou Nutrition and Health Study. Face-to-face interviews using a seventy-nine-item FFQ were administered to assess habitual dietary flavonoid intake, while abdominal ultrasonography was conducted to evaluate the presence and degree of NAFLD, with measurements conducted 3 years apart. After adjustment for potential confounders, higher flavonoid intakes were gradely associated with reduced risks of worsen NAFLD status. The relative risks of worsening (v. non-worsening) NAFLD in the highest (v. lowest) quintile were 0·71 (95 % CI 0·54, 0·93) for total flavonoids, 0·74 (95 % CI 0·57, 0·95) for flavanones, 0·74 (95 % CI 0·56, 0·96) for flavan-3-ols, 0·90 (95 % CI 0·68, 1·18) for flavonols, 0·73 (95 % CI 0·56, 0·93) for flavones, 0·79 (95 % CI 0·61, 1·02) for isoflavones and 0·74 (95 % CI 0·57, 0·96) for anthocyanins. An L-shaped relationship was observed between total flavonoid intake and the risk of NAFLD progression. Path analyses showed that the association between flavonoids and NAFLD progression was mediated by decreases in serum cholesterol and homeostasis model assessment of insulin resistance. This prospective study showed that higher flavonoid intake was associated with a lower risk of NAFLD progression in the elderly overweight/obese Chinese population.
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http://dx.doi.org/10.1017/S0007114520002846DOI Listing
February 2021

Efficient Electrochemical Nitrogen Fixation over Isolated Pt Sites.

Small 2020 Jun 27;16(22):e2000015. Epub 2020 Apr 27.

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), State Key Laboratory of Elemento-Organic Chemistry, School of Materials Science and Engineering, College of Chemistry, Nankai University, Tianjin, 300071, People's Republic of China.

Recently, ambient electrochemical N fixation has gained great attention. However, the commercial Pt-based electrocatalyst hardly shows its potential in this field. Herein, it is found that the isolated Pt sites anchored on WO nanoplates exhibit the optimum electrochemical NH yield rate (342.4 µg h mg ) and Faradaic efficiency (31.1%) in 0.1 m K SO at -0.2 V versus RHE, which are about 11 and 15 times higher than their nanoparticle counterparts, respectively. The mechanistic analysis indicates that N conversion to NH follows an alternating hydrogenation pathway, and positively charged isolated Pt sites with special Pt-3O structure can favorably chemisorb and activate the N . Furthermore, the hydrogen evolution reaction can be greatly suppressed on isolated Pt sites decorated WO nanoplates, which guarantees the efficient going-on of nitrogen reduction reaction.
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http://dx.doi.org/10.1002/smll.202000015DOI Listing
June 2020

Reconstruction of Paprosky type IIIB acetabular bone defects using a cup-on-cup technique: A surgical technique and case series.

World J Clin Cases 2020 Apr;8(7):1223-1231

Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Background: Paprosky type IIIB acetabular bone defects are very difficult to reconstruct. For severe defects, we developed our own cup-on-cup technique. We defined the tantalum metal (TM) revision shell with the peripheral titanium ring removed as a TM-cup augment and the cementless hemispherical acetabulum component combined with a TM-cup augment as the cup-on-cup technique.

Aim: To report the short-term results of patients with type IIIB acetabular bone defects reconstructed using the cup-on-cup technique.

Methods: We retrospectively reviewed six patients (six hips) with a mean age of 59 years who underwent acetabular reconstruction using our cup-on-cup technique between January 2015 and January 2017. All acetabular bone defects were classified as type IIIB without pelvic discontinuity using the system of Paprosky. All patients were followed both clinically and radiographically for a mean duration of 42 mo.

Results: The mean Harris hip score improved from 32.4 pre-operatively to 80.7 at the last follow-up. The mean vertical position of the hip rotation centre changed from 60.9 mm pre-operatively to 31.7 mm post-operatively, and the mean horizontal position changed from 33.6 mm pre-operatively to 38.9 mm post-operatively. Greater trochanteric migration after extended trochanteric osteotomy occurred in one of six hips at 3 mo. There was no evidence of component migration at the last follow-up.

Conclusion: The short-term results suggest that our cup-on-cup technique could be considered an effective management option for Paprosky type IIIB acetabular bone defects without pelvic discontinuity.
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http://dx.doi.org/10.12998/wjcc.v8.i7.1223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176610PMC
April 2020

[Microbial community diversity and its characteristics in Magnolia Officinalis Cortex "sweating" process based on high-throughput sequencing].

Zhongguo Zhong Yao Za Zhi 2019 Dec;44(24):5405-5412

State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources Chengdu 611137,China Pharmacy College,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.

Magnolia Officinalis Cortex has been used as a traditional Chinese herb for thousands of years in China. According to Chinese Pharmacopoeia,the processing of Magnolia Officinalis Cortex needs " sweating" or " Fahan",which was a special drying process and considered to be an important symbol for high quality and genuine medicinal materials. In this unique processing mode,Magnolia Officinalis Cortex's microbial community structure may be changed,but little is known about microbial diversity during the " sweating". In this study,to analyze the change and its change rules of microbial community of Magnolia Officinalis Cortex in the whole process of " sweating",and find out the microbial community that affects the quality of Magnolia Officinalis Cortex in the process of its " sweating",and provide a basis for further research on the microbial transformation of Magnolia Officinalis Cortex,MiSeq highthroughput sequencing was used to evaluate the microbial diversity of natural " sweating" of Magnolia Officinalis Cortex. In this research,334 genera fungi and 674 genera bacteria were identified. The dominant species weren' t obvious during the early stage of " sweating". Candida was the dominant fungal species( 45. 01%-71. 93%) during the medium " sweating" stage. Aspergillus is the dominant fungal species( 45. 83%-95. 51%) during the late stage of " sweating". Moreover,Enterobacter and Klebsiella were the primary bacterial genus( ≥56. 05%) during the middle and late stages of " sweating". In addition,the predominant bacteria in the process of " sweating" included Bacillus,Deinococcus,Sphingomonas,Hymenobacter and Jatrophihabitans. In conclusion,the microbial diversities and the main dominant fungi and bacteria in the process of " sweating" of Magnolia Officinalis Cortex were initially determined. It was also found that the metabolism of Aspergillus and Candida may be related to the character formation,which were sweet odor and brown inner surface after " sweating". The results provide a theoretical basis for the study of the influence of different microorganisms on the excellent traits formation of " sweating" Magnolia Officinalis Cortex.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20190916.305DOI Listing
December 2019

Association between metabolic body composition status and risk for impaired renal function: A cross-sectional study.

PLoS One 2019 26;14(11):e0223664. Epub 2019 Nov 26.

College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Background: The risk for obesity-related disorders is proportional to the visceral region and had been observed to be highly related with impaired renal function. In the current study, we aimed to evaluate renal function impairment, according to sex, age, and different status of metabolic body composition.

Methods: We retrospectively collected from the medical records the basic information and metabolic titers of Chinese adults (13,373 men and 10,175 women) who underwent health checkup from 2013 to 2016. The population was divided into four groups, according to metabolic body composition, including metabolic healthy norms-weight (MHNW), metabolic healthy obesity (MHO), metabolic unhealthy norms-weight (MUNW), and metabolic unhealthy obesity (MUO). The categorical data were compared among the groups and logistic regression analyses were conducted to investigate the association between metabolic body composition status and risk for renal function impairment.

Results: Across all ages in both sexes, the odds ratios (OR) for renal function impairment were higher in the MHO, MUNW, and MUO groups than in the MHNW group, except for women <45 years old in the MUNW group. However, after adjustment, the trend was no longer significant in all groups under 45 years old. For individuals >45 years old, the relatively high risk for renal function impairment remained significantly associated with the MUNW group (OR 2.95, 95% CI 2.02-4.30 in men and OR 1.95, 95% CI 1.35-2.82 in women) and MUO group (OR 2.33, 95% CI 1.82-3.00 in men and OR 2.67, 95% CI 2.04-3.48 in women).

Conclusion: Impaired renal function was independently associated with the status of metabolic obesity. However, the trend was only observed in individuals >45 years old, with significant sex difference.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223664PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879116PMC
March 2020

Retrieval of a Broken Sewing Needle from the Sacrum Aided by a Permanent Magnet: A Case Report and Literature Review.

Open Med (Wars) 2017 29;12:446-449. Epub 2017 Dec 29.

Department of Trauma Orthopedics, Tengzhou Central People's Hospital, Jining Medical College, Tengzhou, China.

Penetrating sacral injuries are very rare, but foreign bodies that penetrate the sacrum often cause pain, cerebrospinal fluid leakage, and neurological symptoms. Careful preoperative medical and imaging examinations, determining the position of the foreign body, and surgical exploration are essential in the management of such cases. We present the case of a woman who visited our emergency department with a broken sewing needle in her sacrum. The incident occurred when the patient accidentally sat on the needle while sewing by hand. Pain while sitting was the main clinical finding after the accident. The patient was treated successfully by surgical exploration aided by a permanent magnet. At follow-up a few months later, she was symptom-free. In clinical practice physicians often encounter patients with metallic foreign bodies. Permanent magnets play an important role in the removal of metallic foreign bodies, and emergency department physicians should master the methods and techniques.
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http://dx.doi.org/10.1515/med-2017-0063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757347PMC
December 2017

Hemorrhagic pleural effusion related to acquired coagulation factor VIII deficiency: A case report.

Exp Ther Med 2017 Dec 18;14(6):6084-6086. Epub 2017 Oct 18.

Department of Cardiology, Xiamen Chang Gung Hospital, Xiamen, Fujian 361000, P.R. China.

A patient with acquired hemophilia A (AHA) with hemorrhagic pericardial effusions was admitted to Xiamen Chang Gung Hospital (Xiamen, China) in August 2015. The patient had been experiencing progressive dyspnea for 1 week. Bloody effusion (~6.3 l) was drained from the membrane surrounding the heart over a period of 20 days. Biochemical, cytological and radiological examinations were unable to elucidate the reason for the effusion. Coincidentally, it was discovered that activated partial thromboplastin time prolongation could not be corrected by plasma mixing. Furthermore, immunologic and functional assays identified that the patient had factor VIII-deficient plasma. Finally, the coagulopathy was treated by infusion of cryoagglutinin and steroids to eradicate the coagulation inhibitor. The production of cardiac bloody effusions did not recur. Notably, the patient was diagnosed with AHA accompanied by the rare complication of pericardial effusions. The present case was the first to report AHA with the complication of pericardial effusions.
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http://dx.doi.org/10.3892/etm.2017.5339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740789PMC
December 2017

Development of SSR markers from transcriptomes for (Poaceae), an endemic of the Qinghai-Tibetan Plateau.

Appl Plant Sci 2017 Jul 19;5(7). Epub 2017 Jul 19.

Department of Plant Science, Quaid-i-Azam University, Islamabad 44000 Pakistan.

Premise Of The Study: Transcriptomes were used to develop microsatellite markers for the plant genus (Poaceae), which comprises three species of grasses (. , . , and . ) that are widely distributed in the Qinghai-Tibetan Plateau.

Methods And Results: Primer pairs were developed for 16 high-quality simple sequence repeats (SSRs) using transcriptomes. SSRs were amplified in 248 individuals representing the three species of ; the number of alleles per locus ranged from one to seven, with an average of 2.6. The expected and observed heterozygosity per locus varied from 0.00 to 0.83 and from 0.00 to 1.00, respectively, with respective mean values of 0.32 and 0.34.

Conclusions: These newly developed SSR markers will be valuable for evaluating the population genetic structure of throughout its range.
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http://dx.doi.org/10.3732/apps.1700029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546167PMC
July 2017

Debridement in chronic osteomyelitis with benign osteopetrosis: A case report.

Exp Ther Med 2016 Nov 15;12(5):2811-2814. Epub 2016 Sep 15.

Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian 361000, P.R. China.

Osteopetrosis is a rare bone disease caused by metabolic imbalances as a result of genetic mutations. For instance, autosomal dominant osteopetrosis is caused by a missense mutation of the C1CN7 gene. This was first reported in 1904 and is thought to be caused by osteoclastic dysfunction and an impaired bone resorption ability. An accumulation of cortical bone mass during the remodeling of the medullary bone may increase the bone density and give rise to a hard marble consistency. Osteopetrosis can be divided into benign and malignant forms; however, no curative treatment exists for benign osteopetrosis. The management of complications, such as chronic osteomyelitis and fractures, serves a key role in influencing the patient survival rates. Previous studies have demonstrated that a combined treatment of hyperbaric oxygen (HBO) lavage for debridement of the necrotic region and high-dose systemic antibiotics may be effective in the management of osteopetrosis. The present study reported a case of chronic mandible osteomyelitis and fistula occurring in association with maxillary sinusitis, who was successfully treated by through nasal endoscopy, using repeated flushing and cleaning every 2 weeks as a form of debridement, in the absence of high-dose antibiotics and HBO.
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http://dx.doi.org/10.3892/etm.2016.3706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103689PMC
November 2016

Absorption Characteristics and Environmental Significance of Dissolved Organic Matter in Lake Dongping.

Guang Pu Xue Yu Guang Pu Fen Xi 2016 Oct;36(10):3232-6

As an important water source in Shandong Province, Lake Dongping is one of the most important inland lakes to South-to-North Water Diversion East Route Project. We tried to provide the basis for real-time monitoring of water quality mutation by using CDOM absorption characteristics. The average values of CDOM absorption coefficients [a(280),a(350),a(440)] were (12.90±1.17),(3.11±0.40) and (0.65±0.09) m-1. a(440) had linear relationships with total nitrogen (p<0.01), total phosphorus (p<0.001), dissolved organic carbon (p<0.001), chemical oxygen demand (p<0.001), Chlorophyll a (p<0.001), which can be used to estimate the water quality parameters. The results showed that CDOM absorption coefficient reflects the nutritional status of Lake Dongping, which is close to eutrophication level. There was an obvious decrease of CDOM absorption coefficients from river mouth of Dawen River to lake center and then to the outlets of lake. This illustrated that terrestrial input is the main source of nutrients and pollutants in Lake Dongping.
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October 2016

Genetic association of COL1A1 polymorphisms with high myopia in Asian population: a Meta-analysis.

Int J Ophthalmol 2016 18;9(8):1187-93. Epub 2016 Aug 18.

Sichuan Provincial Key Laboratory for Disease Gene Study, Hospital of University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China; School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China; Health Management Center, Hospital of University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China.

Aim: To comprehensively evaluate the potential association of COL1A1 polymorphisms with high myopia by a systematic review and Meta-analysis.

Methods: All association studies on COL1A1 and high myopia reported up to June 10, 2014 in PubMed, Embase, Web of Science, and the Chinese Biomedical Database were retrieved. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were analyzed for single-nucleotide polymorphisms (SNPs) using fixed- and random- effects models according to between-study heterogeneity. Publication bias analyses were conducted by Egger's test.

Results: A total of four studies from reported papers were included in this analysis. The Meta-analyses for COL1A1 rs2075555, composed of 2304 high myopia patients and 2272 controls, failed to detect any significant association with high myopia. A total of 971 cases and 649 controls were tested for COL1A1 rs2269336. The association of COL1A1 rs2269336 with high myopia was observed in recessive model (CC vs CG+GG, P=0.03) and in heterozygous model (CG vs GG, P=0.04), but not in other models.

Conclusion: This Meta-analysis shows that COL1A1 rs2269336 (CC vs CG+GG) affects individual susceptibility to high myopia, whereas there is no association detected between SNPs rs2075555 and high myopia. Given the limited sample size, further investigations including more ethnic groups are required to validate the association.
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http://dx.doi.org/10.18240/ijo.2016.08.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4990585PMC
September 2016

Di (2-ethylhexyl) phthalate impairs steroidogenesis in ovarian follicular cells of prepuberal mice.

Arch Toxicol 2017 Mar 12;91(3):1279-1292. Epub 2016 Jul 12.

Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China.

Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer which is widely used in the manufacture of plastics. As a common environmental contaminant and recognized endocrine disrupting chemical, DEHP is able to deregulate the functions of a variety of tissues, including the reproductive system both in males and females. In order to investigate the possible effects of DEHP on the first wave of folliculogenesis, occurring in the mouse ovary postnatally, mice were administered 20 or 40 μg/kg DEHP through intraperitoneal injection at days 5, 10 and 15 post partum (dpp). Following DEHP treatment the gene expression profile of control and exposed ovaries was compared by microarray analyses at 20 dpp. We found that in the exposed ovaries DEHP significantly altered the transcript levels of several immune response and steroidogenesis associated genes. In particular, DEHP significantly decreased the expression of genes essential for androgen synthesis by theca cells including Lhcgr, Cyp17a1, Star and Ldlr. Immunohistochemistry and immune flow cytometry confirmed reduced expression of LHCGR and CYP17A1 proteins in the exposed theca cells. These effects were associated to a significant reduction in ovarian concentrations of progesterone, 17β-estradiol and androstenedione along with a reduction of LH in the serum. Although we did not find a significant reduction of the number of primary, secondary or antral follicles in the DEHP exposed ovaries when compared to controls, we did observe that theca cells showed an altered structure of the nuclear envelope, fewer mitochondria, and mitochondria with a reduced number of cristae. Collectively, these results demonstrate a deleterious effect of DEHP exposure on ovarian steroidogenesis during the first wave of folliculogenesis that could potentially affect the correct establishment of the hypothalamic-pituitary-ovarian axis and the onset of puberty.
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http://dx.doi.org/10.1007/s00204-016-1790-zDOI Listing
March 2017

Pleiotropic effects of herbs characterized with blood-activating and stasis-resolving functions on angiogenesis.

Chin J Integr Med 2016 Oct 29;22(10):795-800. Epub 2016 Jun 29.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.

Accumulative evidences have underpinned the nature candidates from Chinese medicine (CM), particularly CM served as blood activating and stasis resolving (BASR, Huoxue Huayu in Chinese) by targeting tumor-associated angiogenesis. However, recent experiment research on the therapeutic angiogenesis by BASR-CM attracts wide attention and discussion. This opinion review focused on the underlying link between two indications and anticipated that (1) BASR-CM might emphasize on a balanced multi-cytokines network interaction; (2) BASR-CM might address on the nature of diseases prior to differently affecting physiological and pathological angiogenesis; (3) BASR-CM might mainly act on perivascular cells, either promotes arteriogenesis by increasing arteriogenic factors in ischemic diseases, or simultaneously keep a quiescent vasculature to impede angiogenesis in tumor context.
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http://dx.doi.org/10.1007/s11655-015-2405-xDOI Listing
October 2016

[Absorption and metabolism of icariin in different osteoporosis rat models].

Zhongguo Zhong Yao Za Zhi 2016 Jul;41(13):2532-2537

Research Center of Multi-component of Chinese Materia Medica and Microecology, Jiangsu Branch, China Academy of Traditional Chinese Medicine, Nanjing 210028, China.

To compare the intestinal absorption and metabolism of icariin in different osteoporosis rat models. Ovariectomy and intragastric administration of cyclophosphamide were used to establish two kinds of rat osteoporosis models. Then the rat intestinal perfusion was conducted, and HPLC was used to measure and calculate the permeability coefficients of icariin in different intestines and production amount of metabolites. Western blot was used to detect LPH enzyme expression in two models. Experimental results showed that both ovariectomy and intragastric administration of cyclophosphamide 4.5 mg•kg⁻¹ could reduce rat bone density and successfully construct the rat osteoporosis models. The apparent permeability coefficient Papp of 20 μmol icariin in duodenum, jejunum, ileum, colon was 5.695, 5.224, 1.492, 0.520 respectively in sham operation group; 3.876, 3.608, 0.863, and 0.291 in ovariectomized group; 4.945, 3.601, 1.990, 1.042 in normal saline group; 3.301, 2.108, 1.209, 1.233 in cyclophosphamide-induced osteoporosis model group. In addition, the protein expression levels of LPH enzyme in two model groups were lower than those in normal group. The absorption and metabolism of icariin in two kinds of osteoporosis models was lower than that in sham operation group and normal saline group; the reduction of expression level of LPH enzymes in rat intestine of different osteoporosis models was one of the reasons for leading to the reduced intestinal absorption and metabolism of icariin.
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http://dx.doi.org/10.4268/cjcmm20161327DOI Listing
July 2016

Preparation of novel butyryl galactose ester-modified coix component microemulsions and evaluation on hepatoma-targeting in vitro and in vivo.

Drug Deliv 2016 Nov 10;23(9):3444-3451. Epub 2016 Jun 10.

a Multicomponent of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine , Nanjing , China and.

The butyryl galactose ester-modified coix component microemulsions (But-Gal-CMEs) was developed for enhanced liver tumor-specific targeting. The study was aimed to evaluate the hepatoma-targeting potential of But-Gal-CMEs in vitro and in vivo. But-Gal-CMEs with a uniform spherical shape exhibited a small particle size (56.68 ± 0.07 nm), a narrow polydispersity (PDI, 0.144 ± 0.005) and slightly negative surface charge (-0.102 ± 0.008 mV). In the cell uptake studies, But-Gal-CMEs showed a significant enhancement on the intracellular fluorescent intensity on HepG2 cells model, which was 1.93-fold higher relative to coix component microemulsions (CMEs). The IC of But-Gal-CMEs against HepG2 cells was 64.250 μg/mL, which was notably stronger than that of CMEs. In the cell apoptosis studies, compared with CMEs, But-Gal-CMEs (50 μg/mL) treatment resulted in a 1.34-fold rise in total apoptosis cells of HepG2. In the biodistribution studies in vivo, the intratumorous fluorescence of Cy5-loaded But-Gal-CMEs was 1.43-fold higher relative to that of Cy5-loaded CMEs, suggesting an obviously enhanced accumulation in the tumor sites. Taken as together, But-Gal could be incorporated into the coix component microemulsions as a novel ligand for realizing hepatoma-targeting drugs delivery.
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http://dx.doi.org/10.1080/10717544.2016.1189984DOI Listing
November 2016

Additional note on using suprapatellar nailing treating tibial fractures.

Authors:
Yu-Ping Liu

Injury 2016 06 27;47(6):1363-4. Epub 2016 Feb 27.

Department of Orthopedics, Tengzhou Central People's Hospital, Tengzhou, Shandong, China. Electronic address:

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http://dx.doi.org/10.1016/j.injury.2016.02.012DOI Listing
June 2016

TWEAK/Fn14 signaling: a promising target in intervertebral disc degeneration.

Histol Histopathol 2016 Sep 24;31(9):943-8. Epub 2016 Feb 24.

Department of Orthopedics, Huaian First People's Hospital, Huaian Jiangsu, P.R. China.

Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a potent chemoattractant cytokine with various biological functions, such as stimulation of angiogenesis, induction of proinflammatory cytokines, regulation of cellular proliferation and apoptosis. Therefore, it has also been implicated in several pathological processes, from cancer to inflammatory diseases. Remarkably, TWEAK and its receptors, fibroblast growth factor inducible 14 (Fn14), are also present in intervertebral disc (IVD) tissue, where they play a role in the pathogenesis of IVD degeneration. The interaction of TWEAK with Fn14 is involved in physiological and pathological activities of IVD degeneration patients, which includes apoptosis of endplate chondrocytes, extracellular matrix degradation, reduction in proteoglycan synthesis and so on. The blockade of this interaction results in suppressing over-production of proinflammatory factors and cell death in in vivo or in vitro experiments, suggesting that TWEAK/Fn14 signaling may be therapeutically relevant in IVD degeneration, and the targeting of TWEAK or Fn14 has been proposed as a potential therapeutic approach for autoimmune diseases such as Rheumatoid arthritis (RA). In this article, we discuss the biological features of TWEAK/Fn14 signaling and summarize recent advances in our understanding of the role of TWEAK/Fn14 signaling in the pathogenesis and treatment of IVD degeneration. We think that the blockade of TWEAK/Fn14 signaling may be a promising therapeutic strategy for IVD degeneration in the near future.
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http://dx.doi.org/10.14670/HH-11-775DOI Listing
September 2016

Norcantharidin inhibits renal interstitial fibrosis by downregulating PP2Ac expression.

Am J Transl Res 2015 15;7(11):2199-211. Epub 2015 Nov 15.

Department of Nephrology, Second Xiangya Hospital, Central South University Changsha, Hunan, PR. China.

Norcantharidin (NCTD) has been proven to be able to attenuate renal interstitial fibrosis, but the exact molecular mechanism is still unknown. This study investigated the relationship between the anti-fibrotic effect of NCTD and its inhibition on PP2Ac expression. Here, PP2Ac was found to be positively correlated with extracellular matrix accumulation in the rat unilateral ureteral obstruction (UUO) model. Additional experiments showed that the PP2A inhibitor (okadaic acid) can ameliorate renal interstitial fibrosis by inhibiting the expression of fibronectin (FN) and collagen I (Col-I) and reversing tubular epithelial-to-mesenchymal transition in vivo and in vitro. In vitro experiments also demonstrated that ectopic over-expression of PP2Ac has a profibrotic effect in HK-2 cells. Moreover, NCTD was able to downregulate PP2Ac expression, decrease FN, Col-I, α-SMA expression, and increase E-cadherin expression in a dose-dependent manner both in vivo and in vitro. In particular, it was demonstrated that NCTD induced no evident changes in the expression of FN, Col-I, α-SMA and E-cadherin in HK-2 cells after PP2Ac was knocked down by shRNA. These results indicated that NCTD exerts an anti-fibrosis effect via inhibition of PP2Ac expression. Thus, PP2Ac could be a promising target for intervention in renal interstitial fibrosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697700PMC
January 2016
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