Publications by authors named "Yu Mao"

257 Publications

Two-phase activated colorimetric and ratiometric fluorescent sensor for visual detection of phosgene via AIE coupled TICT processes.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 16;253:119589. Epub 2021 Feb 16.

School of Chemistry and Chemical Engineering, Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, 28 Changsheng West Road, Hengyang, Hunan 421001, PR China. Electronic address:

In this paper, we specifically designed and synthesized an excellent colorimetric and ratiometric fluorescent sensor DPA-CI for rapid and convenient detection of the highly toxic phosgene. DPA-CI was developed by incorporated a diphenylamine (DPA) and a 2-imine-3-benzo[d]imidazole as the enhanced push-pull electronic structure into the coumarin fluorophore matrix. The sensor DPA-CI towards phosgene sensing exhibited both visible colorimetric and ratiometric fluorescent color change in solution and in gaseous conditions with TICT and AIE mechanism respectively, which can be easily distinguished by using the naked eye. Also, the sensor DPA-CI showed splendid sensing performance such as excellent selectivity, rapid response (less than 8 s in THF and 2 min in gaseous condition), and fair sensitivity (limit of detection less than 0.11 ppm in gaseous condition and 0.27 μM in solution). The design strategy based on enhanced push-pull electronic structure with AIE and TICT properties will be helpful to construct a solid optical sensor with excellent potential application prospects for portable and visual sensing of gaseous phosgene through distinct color and ratiometric fluorescence change by the naked eyes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119589DOI Listing
February 2021

FEAST of biosensors: Food, environmental and agricultural sensing technologies (FEAST) in North America.

Biosens Bioelectron 2021 Apr 21;178:113011. Epub 2021 Jan 21.

Biological Engineering, Utah State University, USA. Electronic address:

We review the challenges and opportunities for biosensor research in North America aimed to accelerate translational research. We call for platform approaches based on: i) tools that can support interoperability between food, environment and agriculture, ii) open-source tools for analytics, iii) algorithms used for data and information arbitrage, and iv) use-inspired sensor design. We summarize select mobile devices and phone-based biosensors that couple analytical systems with biosensors for improving decision support. Over 100 biosensors developed by labs in North America were analyzed, including lab-based and portable devices. The results of this literature review show that nearly one quarter of the manuscripts focused on fundamental platform development or material characterization. Among the biosensors analyzed for food (post-harvest) or environmental applications, most devices were based on optical transduction (whether a lab assay or portable device). Most biosensors for agricultural applications were based on electrochemical transduction and few utilized a mobile platform. Presently, the FEAST of biosensors has produced a wealth of opportunity but faces a famine of actionable information without a platform for analytics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2021.113011DOI Listing
April 2021

Solution-gated graphene transistor based sensor for histamine detection with gold nanoparticles decorated graphene and multi-walled carbon nanotube functionalized gate electrodes.

Food Chem 2021 Jun 6;347:128980. Epub 2021 Jan 6.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Research Laboratory of Agricultural Environment and Food Safety, Anhui Modern Agricultural Industry Technology System, Hefei 230009, China. Electronic address:

In order to achieve accurate detection and evaluation of the freshness of fish samples, high sensitivity and selectivity of histamine sensors based on solution-gated graphene transistors (SGGT) have been successfully developed. By using graphene (Gra), multi-walled carbon nanotubes (MWNT) and gold nanoparticles (AuNP) to functionalize the gate electrode, the electrocatalytic performance of the device can be significantly improved. We have found that graphene, MWNT and AuNP modified SGGT sensors exhibit an ultra-low detection limit of 100 nM for histamine, a linear range of 3 μM-100 μM. We have also demonstrated that the SGGT-based histamine sensor has a high recovery rate and is capable of assessing the histamine content of actual fish samples in a fast and accurate manner. Considering the superior performance of the SGGT-based histamine sensor, it can be readily extended to histamine determination in many other real food samples for their freshness assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.128980DOI Listing
June 2021

Switching of delta opioid receptor subtypes in central amygdala microcircuits is associated with anxiety states in pain.

J Biol Chem 2021 Jan 8:100277. Epub 2021 Jan 8.

School of Basic Medical Sciences, Anhui Medical University, Meishan Road 81, Hefei 230022, China; Hefei National Laboratory for Physical Sciences at the Microscale, Department of Biophysics and Neurobiology, University of Science and Technology of China, Hefei 230027, PR China. Electronic address:

Anxiety is often comorbid with pain. Delta opioid receptors (DORs) are promising targets for the treatment of pain and mental disorders with little addictive potential. However, their roles in anxiety symptoms at different stages of pain are unclear. In the current study, mice with inflammatory pain at the 4th hour following complete Freund's adjuvant (CFA) injection displayed significant anxiety-like behavior, which disappeared at the 7th day. Combining electrophysiology, optogenetics, and pharmacology, we found that activation of delta opioid receptor 1 (DOR1) in the central nucleus amygdala (CeA) inhibited both the anxiolytic excitatory input from the basolateral amygdala (BLA) and the anxiogenic excitatory input from the parabrachial nucleus (PBN). In contrast, activation of delta opioid receptor 2 (DOR2) did not affect CeA excitatory synaptic transmission in normal and 4-hour CFA mice but inhibited the excitatory projection from the PBN rather than the BLA in 7-day CFA mice. Furthermore, the function of both DOR1 and DOR2 was downregulated to the point of not being detectable in the CeA of mice at the 21st day following CFA injection. Taken together, these results suggest that functional switching of DOR1 and DOR2 is associated with anxiety states at different stages of pain via modulating the activity of specific pathways (BLA-CeA and PBN-CeA).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbc.2021.100277DOI Listing
January 2021

Single-atom nanozyme enabled fast and highly sensitive colorimetric detection of Cr(VI).

J Hazard Mater 2021 Apr 18;408:124898. Epub 2020 Dec 18.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Intelligent Interconnected Systems Laboratory of Anhui Province, Hefei University of Technology, Hefei 230009, China. Electronic address:

As a high biologically toxic heavy metal ion, Cr(VI) will cause environmental pollution and endanger human health. Therefore, the development of fast, simple and visible detection methods for Cr(VI) is extremely important to control its harm. Toward this end, we report the establishment of a colorimetric sensing method for Cr(VI) based on single-atom nanozymes for enhanced detection performance. Firstly, we prepared SA-Fe/NG as peroxidase mimetic by anchoring Fe single-atom onto a single-layer of two-dimensional nitrogen-doped graphene. The SA-Fe/NG showed superiorly high oxidation catalytic activity due to its 100% atomic utilization and existing Fe-N-C structure. Furthermore, with 3,3',5,5'-tetramethylbenzidine (TMB) as a colorimetric sensing probe, and 8-hydroxyquinoline (8-HQ) as an inhibitor for the oxidation of TMB, the detection of Cr(VI) was realized through specific interaction between Cr(VI) and 8-HQ, which led to the recovery of oxTMB in blue color. Our established method showed superior sensitivity with a detection limit of 3 nM and a linear range of 30 nM to 3 μM. It also exhibited high selectivity for a series of metal cations, and has been successfully applied to the detection of Cr(VI) in tap water and tuna samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124898DOI Listing
April 2021

Machinability of the Thermoplastic Polymers: PEEK, PI, and PMMA.

Polymers (Basel) 2020 Dec 26;13(1). Epub 2020 Dec 26.

Key Laboratory for Precision and Non-Traditional Machining Technology of Ministry of Education, Dalian University of Technology, Dalian 116024, China.

The thermoplastic polymer such as poly(methyl methacrylate) (PMMA), polyetheretherketone (PEEK), and polyimide (PI) is a kind of polymer material with properties of good mechanical strength. It has been widely used in the fields of aerospace, optical engineering, and microfluidics, etc. Thermoplastic polymers are considered to be one of the most promising engineering plastics in the future. Therefore, it is necessary to further study its mechanical properties and machinability, especially in ultra-precision machining. Furthermore, mechanical property and machinability were studied in this work. Through the dynamic mechanical analysis experiment, the elastic modulus and temperature effect of PMMA, PEEK, and PI are analyzed. In addition, the high-speed micromilling experiment is conducted to show that the surface roughness, burrs, and cutting chip characteristics in the high-speed micromilling process. In general, the surface quality of the brittle removal is generally better than that of the viscoelasticity state. The results show that PMMA, PEEK, and PI have good mechanical properties and machinability. Base on the results, the material will be in a viscoelastic state as the temperature increases. The surface quality of the brittle removal is generally better than the viscoelastic state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym13010069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796128PMC
December 2020

Connectome-based evidence for creative thinking as an emergent property of ordinary cognitive operations.

Neuroimage 2021 02 11;227:117632. Epub 2020 Dec 11.

Key Laboratory of Cognition and Personality (SWU), Ministry of Education, Chongqing 400715, China; School of Psychology, Southwest University (SWU), Chongqing 400715, China; Southwest University Branch, Collaborative Innovation Center of Assessment Toward Basic Education Quality at Beijing Normal University, Chongqing 400715, China. Electronic address:

Creative thinking is a hallmark of human cognition, which enables us to generate novel and useful ideas. Nevertheless, its emergence within the macro-scale neurocognitive circuitry remains largely unknown. Using resting-state fMRI data from two large population samples (SWU: n = 931; HCP: n = 1001) and a novel "travelling pattern prediction analysis", here we identified the modularized functional connectivity patterns linked to creative thinking ability, which concurrently explained individual variability across ordinary cognitive abilities such as episodic memory, working memory and relational processing. Further interrogation of this neural pattern with graph theoretical tools revealed both hub-like brain structures and globally-efficient information transfer paths that together may facilitate higher creative thinking ability through the convergence of distinct cognitive operations. Collectively, our results provide reliable evidence for the hypothesized emergence of creative thinking from core cognitive components through neural integration, and thus allude to a significant theoretical advancement in the study of creativity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroimage.2020.117632DOI Listing
February 2021

Achieving Ultrasmall Prussian Blue Nanoparticles as High-Performance Biomedical Agents with Multifunctions.

ACS Appl Mater Interfaces 2020 Dec 9;12(51):57382-57390. Epub 2020 Dec 9.

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Prussian blue nanoparticles (PBNPs), which belong to the iron-based metal-organic frameworks, are important biomedical agents. Reducing the size of PBNPs can bring improved functional properties, but unfortunately, has been a long-standing challenge. Herein, sub-5 nm ultrasmall PBNPs (USPBNPs) were successfully synthesized by using ethanol/water mixture as the solvent and polyvinyl pyrrolidone (PVP) as the surface capping agent. Adjusting the ethanol/water ratio is not only able to control the nucleation time and size of PBNPs but also tune the conformation of PVP molecules so as to prevent interparticle attachment and enlargement. At an ethanol/water ratio of 3:1, highly stable USPBNPs with a size of ∼3.4 nm were synthesized. Due to their large specific surface area, they demonstrated high peroxidase-like and catalase-like activities, which outperform PBNPs synthesized by a conventional method. In addition, they also showed a high longitudinal relaxation rate () of 1.3 mM S, suggesting their potential to be used as MRI agent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c18357DOI Listing
December 2020

Efficient synergistic disinfection by ozone, ultraviolet irradiation and chlorine in secondary effluents.

Sci Total Environ 2021 Mar 19;758:143641. Epub 2020 Nov 19.

Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Beijing Laboratory for Environmental Frontier Technologies, Beijing 100084, PR China; Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, PR China. Electronic address:

Disinfection of secondary effluents is vital to provide a sustainable aquatic environment, minimize microbial risks and guarantee public and environmental safety. This study investigated the effectiveness of six treatment trains including single and combined disinfection processes (i.e., ozone alone, ultraviolet (UV) irradiation alone, chlorine alone, sequential ozone-UV, sequential ozone-chlorine and sequential ozone-UV-chlorine) on bacterial inactivation, as well as bulk water quality parameters such as color, turbidity, absorbance at 254 nm (UV), dissolved organic carbon (DOC) and fluorescence based on samples collected from an actual water reclamation plant (WRP). For the single disinfection processes, when the ozone, UV and chlorine doses reached 5 mg/L, 15 mJ/cm and 4 mg/L, respectively, the log removal of Escherichia coli (E. coli) reached 5 log. A trailing phenomenon was observed with further increases in the disinfectant dosage. Under the combined treatment scenarios, ozone pretreatment resulted in substantial removal of color, turbidity, UV, fluorescence excitation-emission matrix (FEEM) and chlorine consuming organics, thus enhancing the efficiency of subsequent UV irradiation or chlorine treatments. In the sequential ozone-UV-chlorine experiments, E. coli inactivation reached 7 log with ozone, UV and available chlorine of 3 mg/L, 5 or 10 mJ/cm and 2.5 mg/L, respectively. On the basis of the results from the actual WRP, the estimated operating cost per unit for the disinfection systems is 0.065 CNY/t, which is economical for long-term operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.143641DOI Listing
March 2021

The internal representation of temporal orienting: A temporal pulse-accumulation and attentional-gating-based account.

Atten Percept Psychophys 2021 Jan 23;83(1):331-355. Epub 2020 Nov 23.

Key Laboratory of Adolescent Cyberpsychology and Behavior (CCNU), Ministry of Education, Wuhan, 430079, China.

Timing can be processed explicitly or implicitly. Temporal orienting is a typical implicit timing through which we can anticipate and prepare an optimized response to forthcoming events. It is, however, not yet clear whether mechanisms such as temporal-pulse accumulation and attentional gating (more attention, more accumulated temporal pulses) underly the internal representations of temporal orienting, as in explicit timing. To clarify this, a dual-task paradigm, consisting of a temporal orienting and an interference task, was adopted. Consistent with the temporal-pulse-accumulation and attentional-gating model, reaction times to the target detection of temporal orienting increased as the interference stimuli were temporally closer to the target, i.e., a location effect for temporal orienting. This effect is likely due to attention being diverted away from temporal orienting to monitor the occurrence of the interference stimuli for a longer time, resulting in greater temporal pulse loss and less accurate temporal orienting for conditions with later interference stimuli. The temporal-pulse-accumulation aspect in temporal orienting received further support by taking an explicit duration reproduction (containing a second temporal-pulse accumulation) as the interference task. On the one hand, temporal orienting became less accurate with increased temporal-pulse-accumulation overlaps between the dual tasks; on the other hand, two-way (one for temporal orienting and the other for duration reproduction), rather than one-way, location effects were observed, implying processing conflicts between the two temporal-pulse accumulations. Taken together, these results suggest that implicit and explicit timing may share common mechanisms upon internal temporal representations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3758/s13414-020-02176-yDOI Listing
January 2021

Rational designing an azo colorimetric sensor with high selectivity and sensitivity for uranium environmental monitoring.

Anal Chim Acta 2020 Dec 20;1140:153-167. Epub 2020 Oct 20.

Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan, 421001, PR China. Electronic address:

Uranium, a double-edged sword with high-efficiency energy and radioactive toxicity, attracts people to enjoy the advantages by generating power, but at the same time brings potential harm by nuclide migration. Therefore, it is necessary to develop a highly-efficient sensor to monitor uranyl ions in the field. However, the designed processes of the reported sensors are random, time-consuming and difficulty. It is urgent to find a new strategy to rationally, quickly and effectively screen out the required molecule for efficient uranium detection. Herein, with the guidance from the rational design method, the parameter, E, was applied to judge the chromatic aberration between the designed PADAP derivate and its uranyl complex. And the optimized structure sensor, 2-((3-bromo-5-(9,9-dioctyl-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9H-fluoren-2-yl)-2-pyridylazo)-5-(diethylamino)phenol (abbr. W1H), was screened and successfully synthesized. W1H exhibited significant color change (red to blue) after binding uranyl ions, and its detection limit for uranium was as low as nanomolar. More meaningfully, the coordination mechanism of W1H toward uranium was clarified by theoretical calculation of the electron cloud density distribution, E, and H NMR, IR and MS for the first time. With the more excellent uranyl sensitivity, selectivity, and recyclability, W1H and its colorimetric test strip have been applied to the detection of low-concentration uranium in environmental samples around the uranium tailings, providing a useful on-site method to monitor trace uranium in actual samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2020.10.005DOI Listing
December 2020

Association between exposure to polyfluoroalkyl chemicals and increased fractional exhaled nitric oxide in adults.

Environ Res 2020 Nov 11:110450. Epub 2020 Nov 11.

The Longgang People's Hospital, Wenzhou Medical University, No.238 Longxiang Road, Longgang City, Zhejiang, 325800, China. Electronic address:

Background: Perfluoroalkyl chemicals (PFCs) are widely detected in the environment and human body, and they have been linked to asthma and a number of respiratory responses in children and mice. However, no previous studies have investigated the association between exposure to PFCs and airway inflammation in adults.

Objectives: To evaluate the associations between serum PFCs and fractional exhaled nitric oxide (FeNO), a biomarker of airway inflammation, in adults.

Methods: A cross-sectional study of 3630 adults aged 20-79 years who participated in the National Health and Nutrition Examination Survey (NHANES, 2007-2012) was conducted. Serum concentrations of five major PFCs were measured using SPE-HPLC-TIS-MS/MS method, including perfluorohexane sulfonic acid (PFHxS), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), and perfluorodecanoic acid (PFDE). The detection rates of them were all >85%. Weighted multivariable linear regression and Bayesian kernel machine regression (BKMR) analyses were applied to examine the associations between serum PFCs and FeNO.

Results: After adjusted for potential confounding factors, linear regression analyses found that compared with their lowest tertiles, highest tertiles of PFOS, PFDE and PFOA were significantly associated with 5.02% (95% CI: 1.40%, 8.77%), 3.77% (95% CI: 0.30%, 7.36%) and 6.34% (95% CI: 2.81%, 10.01%) increases in FeNO, respectively. The second tertile of PFNA was significantly correlated with a 4.79% (95% CI: 1.41%, 8.29%) increase in FeNO compared with the lowest tertile. In the BKMR analysis, the mixture effect of PFCs on FeNO increased significantly when the PFC levels were at or above the 60th percentiles compared to those at their medians. PFOS and PFOA displayed significant positive single-exposure effects on FeNO when all the other PFCs are set at a particular threshold.

Conclusions: This study provided preliminary evidence that serum PFCs were positively associated with increased FeNO in adults.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2020.110450DOI Listing
November 2020

Machine Learning Analysis of Raman Spectra of MoS.

Nanomaterials (Basel) 2020 Nov 9;10(11). Epub 2020 Nov 9.

Laboratory of Micro-Nano Optoelectronic Materials and Devices, Key Laboratory of Materials for High-Power Laser, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China.

Defects introduced during the growth process greatly affect the device performance of two-dimensional (2D) materials. Here we demonstrate the applicability of employing machine-learning-based analysis to distinguish the monolayer continuous film and defect areas of molybdenum disulfide (MoS) using position-dependent information extracted from its Raman spectra. The random forest method can analyze multiple Raman features to identify samples, making up for the problem of not being able to effectively identify by using just one certain variable with high recognition accuracy. Even some dispersed nucleation site defects can be predicted, which would commonly be ignored under an optical microscope because of the lower optical contrast. The successful application for classification and analysis highlights the potential for implementing machine learning to tap the depth of classical methods in 2D materials research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano10112223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695331PMC
November 2020

Clinical implication of QFR in patients with ST-segment elevation myocardial infarction after drug-eluting stent implantation.

Int J Cardiovasc Imaging 2020 Oct 12. Epub 2020 Oct 12.

Department of Cardiology, Tongji Hospital, Tongji University, No. 389, Xincun Rd, Putuo District, Shanghai, 200065, China.

The feasibility and prognostic value of quantitative flow ratio (QFR) after percutaneous coronary intervention (PCI) in ST-segment elevation myocardial infarction (STEMI) patients have not been assessed. The aim of this study was to investigate the prognostic utility of post-PCI QFR to predict outcomes in STEMI and determine the influence of functional results, in both culprit and nonculprit lesions, after PCI. Patients undergoing PCI of culprit lesions and receiving staged procedures of nonculprit lesions after 7 days were enrolled from 2 centers and underwent post-PCI QFR. The primary outcome was the vessel-oriented composite endpoints (VOCEs), defined as vessel-related cardiovascular death, vessel-related myocardial infarction, and target vessel revascularization. Four hundred fifteen vessels (186 culprit lesions and 219 nonculprit lesions) in 186 patients were analyzed. Measured at staged PCI, the post-PCI QFR of culprit lesions was significantly lower than that of nonculprit lesions (0.92 ± 0.10 versus 0.95 ± 0.08, p < 0.001). The multivariable model demonstrated that low post-PCI QFR was an independent predictor of 2-year VOCE (20.8% versus 5.7%; hazard ratio 2.718; 95% CI 1.347-5.486; p = 0.005). In STEMI patients with a low angiography-derived index of microcirculatory resistance (≤ 40U), a good correlation and agreement between post-PCI QFR value of culprit lesions at primary and staged procedures (r = 0.942; mean difference: - 0.0017 [- 0.074 to 0.070]) was identified. In conclusion, culprit lesions suffered from suboptimal functional results more frequently compared to nonculprit lesions after PCI in STEMI patients. Low post-PCI QFR was associated with subsequent adverse clinical outcomes. After stenting, culprit lesions may feasibly be assessed through QFR without significant microvascular dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10554-020-02068-0DOI Listing
October 2020

Low-level environmental lead and cadmium exposures and dyslipidemia in adults: Findings from the NHANES 2005-2016.

J Trace Elem Med Biol 2021 Jan 28;63:126651. Epub 2020 Sep 28.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 288 Nanjing Road, Heping District, Tianjin, 300020, China. Electronic address:

Background: Previous experimental and occupational health studies have shown the toxic effects of relatively high-level cadmium and lead on lipid metabolism. However, limited studies investigated the relationships between serum lipid levels and exposure to low-level lead and cadmium in adults.

Objective: To investigate the associations between lead and cadmium levels in blood and dyslipidemia in adults.

Methods: A retrospective cross-sectional study of 7,457 adults aged 20-79 years who were recruited in the National Health and Nutrition Examination Survey (NHANES, 2005-2016) was conducted. Multivariate linear and logistic regressions were used to examine the associations of blood lead and cadmium levels with serum lipid profiles and risk of dyslipidemia, respectively.

Results: The weighted geometric means [95% confidence intervals (CIs)] of lead and cadmium in blood were 1.23 (1.21, 1.25) μg/dL and 0.36 (0.35, 0.37) μg/L, respectively. Blood lead was significantly associated with serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), non-high-density lipoprotein cholesterol (non-HDL-C), and apolipoprotein B (Apo B) levels after adjusting for covariates. Compared with the adults in the lowest blood lead quartile (≤0.76 μg/dL), those in the highest lead quartile (>1.90 μg/dL) had higher risks of elevated TC (OR = 1.88, 95% CI: 1.59-2.22), non-HDL-C (OR = 1.59, 95% CI: 1.33-1.91), LDL-C (OR = 1.68, 95% CI: 1.41-1.99) and Apo B (OR = 2.00, 95% CI: 1.46-2.73). However, the single effect of cadmium exposure and the joint effect of lead and cadmium exposures on dyslipidemia were not observed.

Conclusion: Blood lead well below the current recommended level was positively associated with the risk of dyslipidemia in adults, while the low-level cadmium exposure currently observed in adults did not show any significant associations with lipid levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtemb.2020.126651DOI Listing
January 2021

Evolution of a highly functional circular DNA aptamer in serum.

Nucleic Acids Res 2020 11;48(19):10680-10690

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.

Circular DNA aptamers are powerful candidates for therapeutic applications given their dramatically enhanced biostability. Herein we report the first effort to evolve circular DNA aptamers that bind a human protein directly in serum, a complex biofluid. Targeting human thrombin, this strategy has led to the discovery of a circular aptamer, named CTBA4T-B1, that exhibits very high binding affinity (with a dissociation constant of 19 pM), excellent anticoagulation activity (with the half maximal inhibitory concentration of 90 pM) and high stability (with a half-life of 8 h) in human serum, highlighting the advantage of performing aptamer selection directly in the environment where the application is intended. CTBA4T-B1 is predicted to adopt a unique structural fold with a central two-tiered guanine quadruplex capped by two long stem-loops. This structural arrangement differs from all known thrombin binding linear DNA aptamers, demonstrating the added advantage of evolving aptamers from circular DNA libraries. The method described here permits the derivation of circular DNA aptamers directly in biological fluids and could potentially be adapted to generate other types of aptamers for therapeutic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkaa800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641760PMC
November 2020

Thermal Effect on Poly(methyl methacrylate) (PMMA) Material Removal in the Micromilling Process.

Polymers (Basel) 2020 Sep 17;12(9). Epub 2020 Sep 17.

Key Laboratory for Precision and Non-traditional Machining Technology of Ministry of Education, Dalian University of Technology, Dalian 116024, China.

Poly(methyl methacrylate) (PMMA) is of growing interest in the application of microfluidic devices and high precision optical elements due to its excellent moldability and formability. Micromilling is one of the micromachining methods which has been extensively used to manufacture polymer components. In this study, a high-speed micromilling method was used to manufacture polymer with high form accuracy and surface quality. The processing temperature effects on the surface quality were investigated in detail. The dynamic mechanical analysis (DMA) experiment was used to study the material mechanical property under different temperatures. According to the DMA results, the PMMA sample is in the glass and viscoelastic state during the milling process. The cutting chips under various processing temperatures are classified into three kinds according to their shapes: roll, sheet, and sinter. The surface roughness of samples with sheet and roll cutting chips is smaller than that of sinter cutting chips. To obtain a better machining bottom surface and edge shape, the processing temperature below 70 °C is recommended according to the results. This work is of great value for the study of polymer removal mechanism and optimization of processing parameters for the industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym12092122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570307PMC
September 2020

Concurrent environmental enrichment and chronic restraint stress: Effects on innate anxiety and depressive-like behavior in male adolescent mice.

Int J Dev Neurosci 2020 Dec 19;80(8):730-736. Epub 2020 Sep 19.

Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China.

Adolescence is a period that exhibits both vulnerability and adaptation to environmental stimulus. This study explored the co-existence effect of environmental enrichment (EE) and restraint stress (RS) on innate anxiety and depressive-like behavior in adolescent mice. Male ICR mice were treated with daily EE and RS (4 h/d or 8 h/d) for 2 or 4 weeks from early adolescence (postnatal day 30) and emotional behaviors were evaluated 24 h after the end of treatment. 4 weeks of 8 h RS treatment decreased immobility time in forced swimming test, demonstrating an antidepressant-like effect. For 2 weeks of treatment, 8 h RS significantly reduced the time spent in the lighted compartment of the light-dark box, indicating an increased anxiety level. These results show that under the present experimental design, RS treatment with different duration could have different effect on mice emotion-related behavior, but there was no interaction between EE and RS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jdn.10061DOI Listing
December 2020

Cajanolactone A, a stilbenoid from cajanus cajan, prevents ovariectomy-induced obesity and liver steatosis in mice fed a regular diet.

Phytomedicine 2020 Nov 24;78:153290. Epub 2020 Jul 24.

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, PR China. Electronic address:

Background: Visceral obesity and fatty liver are prevalent in postmenopausal women. The stilbene-rich extract of Cajanus cajan (L.) Millsp. has been reported to prevent ovariectomy-induced and diet-induced weight gain in animal models, and stilbenoids from C. cajan are thought to have the potential to prevent postmenopausal obesity and fatty liver.

Purpose: Cajanolactone A (CLA) is the main stilbenoid from C. cajan with osteoblastogenic promoting activity. This study investigated the potential of CLA to prevent postmenopausal obesity and fatty liver. Underlying mechanisms were also investigated.

Method: Ovariectomized C57BL/6 mice fed a regular diet were used as mimics of postmenopausal women and given 10, 20, or 40 mg/kg/d of CLA, 0.1 mg/kg/d of estradiol valerate (EV, positive control), or vehicle (OVX) orally for 16 weeks. Mice of the same age subjected to a sham operation were used as control (Sham). Body weights were recorded every 2 weeks for 16 weeks. Body compositions were analyzed via micro-CT. Serum levels of lipids, adipocytokines and aminotransferases were measured using the relevant kits. mRNA levels of genes of interest were detected by RT-qPCR. Proteomic study of perigonadal white adipose tissue (pWAT) was performed using tandem-mass-tags-based proteomic technology combined with Parallel-Reaction-Monitoring (PRM) validation.

Results: CLA showed potential equivalent to that of EV to prevent ovariectomy-induced overweight, obesity, dyslipidemia, liver steatosis and liver dysfunction, but did not prevent uterine atrophy. In the liver, CLA significantly inhibited ovariectomy-induced upregulation in expression of lipogenic genes SREBP-1c and ChREBP, and stimulated the mRNA expression of apolipoprotein B gene ApoB. In pWAT, CLA reversed, or partially reversed ovariectomy-induced downregulation in the expression of a number of metabolism- and mitochondrial-function-related proteins, including Ndufa3, Pcx, Pdhb, Acly, Acaca, Aldh2, Aacs and Echs1. In addition, ovariectomy-inhibited mRNA expression of Pdhb, Aacs, Acsm5, Echs1, and Aldh2 genes in pWAT was also reversed.

Conclusion: CLA was demonstrated to be a potential non-estrogen-like drug candidate for prevention of postmenopausal obesity and fatty liver. The underlying mechanism might involve the inhibition of lipogenesis and promotion of triglycerides output in the liver, and the promotion of metabolism and mitochondrial functions of visceral white adipose tissue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2020.153290DOI Listing
November 2020

Graft survival and endothelial outcomes after penetrating keratoplasty and Descemet stripping automated endothelial keratoplasty: A systematic review and meta-analysis.

Exp Ther Med 2020 Sep 13;20(3):2794-2804. Epub 2020 Jul 13.

Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing Tongren Hospital, Capital Medical University, Beijing 100073, P.R. China.

The present study aimed to compare the outcomes of graft survival, endothelial cell loss and vision improvement between penetrating keratoplasty (PK) and Descemet stripping automated endothelial keratoplasty (DSAEK) for treating corneal endothelium diseases. The PubMed, CENTRAL (Cochrane) and Embase databases were searched for records added until September 20, 2019. The studies considered were two-arm prospective and retrospective studies comparing outcomes of interest between PK and DSAEK. Ultimately, 10 studies were included with a total of 2,634 patients (910 eyes treated with DSAEK; 1,804 eyes treated with PK). Assessment of the summary effect by meta-analysis suggested that, compared with PK treatment, DSAEK was associated with a greater improvement from baseline in best spectacle-corrected visual acuity [difference (diff.) in means of change from baseline=-0.225, 95% CI=-0.341 to -0.109, P<0.001] and a reduced loss of endothelial cell density (diff. in means=-292.05 cells/mm, 95% CI=-419.53 to -146.57 cells/mm, P<0.001). Graft survival rates were similar using either PK or DSAEK (odds ratio=1.005, 95% CI=0.329-3.071, P=0.993). The overall results suggested that DSAEK may have an advantage over PK for corneal endothelial dysfunction in terms of the visual acuity outcome. The absence of definite time frames in the comparisons limits the conclusions on endothelial cell loss and graft survival.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2020.9010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401902PMC
September 2020

Added value of circulating miRNA expression profiling to sonographic TI-RADS classification in the diagnosis of thyroid nodules.

Exp Ther Med 2020 Aug 10;20(2):1589-1595. Epub 2020 Jun 10.

Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450007, P.R. China.

Potential use of sonographic TI-RADS classification combined with circulating miRNA expression profiling in the diagnosis of thyroid nodules was explored. Retrospective analysis was performed on clinical data of 121 patients with thyroid nodules. The biopsy specimens of patients obtained through ultrasound-guided aspiration and blood specimens were evaluated in Zhengzhou Central Hospital Affiliated to Zhengzhou University from June 2018 to June 2019. In addition, the blood specimen test results of 121 healthy volunteers (control group) who underwent physical examination were retrospectively analyzed. Results of sonographic TI-RADS classification and circulating miRNA expression profiling were compared with the pathological results. Of the 212 nodules, 2 fell into TI-RADS category 2 and were diagnosed as benign. Malignant nodules accounted for 4.35, 37.14, 84.78, 93.33 and 96.77% of those nodules that fell into TI-RADS categories 3, 4a, 4b, 4c and 5, respectively. Of the 121 patients, 92.55% had with nodular goiter, 3.31% had inflammatory nodules, 2.48% toxic nodular goiter, 0.83% thyroid cysts and 0.83% thyroid tumors. A nodule that fell into a higher TI-RADS classification category had a higher risk of malignancy. The expression levels of miRNA146b, miRNA187, miRNA375, miRNA-222-3p and miRNA-151a-5p were higher, while the level of miRNA138 was lower, in patients with either benign or malignant thyroid nodules compaed to those in the control group. The expression levels of miRNA146b, miRNA187, miRNA375, miRNA-222-3p and miRNA-151a-5p were higher, while the level of miRNA138 was lower, in patients with malignant thyroid nodules than those in patients with benign thyroid nodule (P<0.05). The AUC of the combined diagnostic method was 0.973, which was significantly different from the AUCs of the individual diagnostic method (P<0.05). In conclusion, sonographic TI-RADS classification combined with circulating miRNA expression profiling can improve the diagnosis of thyroid nodules.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2020.8870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388447PMC
August 2020

Identification and functional analysis of fourteen NR5A1 variants in patients with the 46 XY disorders of sex development.

Gene 2020 Nov 29;760:145004. Epub 2020 Jul 29.

The Key Laboratory for Human Disease Gene Study of Sichuan Province, Prenatal Diagnosis Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China , Chengdu, Sichuan, PR China; School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, PR China. Electronic address:

Human sex determination and differentiation is a complex process, during which NR5A1 plays a central role via the transcriptional regulation of key modulators involved in steroidogenesis. Approximately 8-15% of 46,XY DSD are caused by variants in the NR5A1 gene. Therefore, screening for variants in the NR5A1 gene was performed in a Chinese cohort of sixty-two 46,XY DSD patients with no AR or SRD5A2 variants via next-generation sequencing (NGS). Fourteen variants in the NR5A1 gene were identified in 16 patients from 14 unrelated families, including nine novel variants. These variants included eight heterozygote missense variants, two heterozygote frameshift variants, two heterozygote nonsense variants, one heterozygote nonframeshift deletion-insertion variant, and one homozygous missense variant. Functional assays showed that the transcriptional activity of the 11 variants was significantly reduced. In this study, 11 NR5A1 pathogenic variants were identified. These novel variants further expand the existing spectrum of the NR5A1 variants associated with 46,XY DSD, which will, in turn, assist in the molecular diagnosis of DSD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2020.145004DOI Listing
November 2020

Identification of glioblastoma-specific prognostic biomarkers via an integrative analysis of DNA methylation and gene expression.

Oncol Lett 2020 Aug 11;20(2):1619-1628. Epub 2020 Jun 11.

Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.

Glioblastoma (GBM) is the most aggressive and lethal tumor of the central nervous system. The present study set out to identify reliable prognostic and predictive biomarkers for patients with GBM. RNA-sequencing data were obtained from The Cancer Genome Atlas database and DNA methylation data were downloaded using the University of California Santa Cruz-Xena database. The expression and methylation differences between patients with GBM, and survival times <1 and ≥1 year were investigated. A protein-protein interaction network was constructed and functional enrichment analyses of differentially expressed and methylated genes were performed. Hub genes were identified using the Cytoscape plug-in cytoHubba software. Survival analysis was performed using the survminer package, in order to determine the prognostic values of the hub genes. The present study identified 71 genes that were hypomethylated and expressed at high levels, and four genes that were hypermethylated and expressed at low levels in GBM. These genes were predominantly enriched in the 'JAK-STAT signaling pathway', 'transcriptional misregulation in cancer' and the 'ECM-receptor interaction', which are associated with GBM development. Among the 24 hub genes identified, 15 possessed potential prognostic value. An integrative analysis approach was implemented in order to analyze the association of DNA methylation with changes in gene expression and to assess the association of gene expression changes with GBM survival time. The results of the present study suggest that these 15 CpG-based genes may be useful and practical tools in predicting the prognosis of patients with GBM. However, future research on gene methylation and/or expression is required in order to develop personalized treatments for patients with GBM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2020.11729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377174PMC
August 2020

OFC and its connectivity with amygdala as predictors for future social anxiety in adolescents.

Dev Cogn Neurosci 2020 08 8;44:100804. Epub 2020 Jun 8.

Key Laboratory of Cognition and Personality (SWU), Ministry of Education, Chongqing, 400715, China; Department of Psychology, Southwest University, Chongqing, 400715, China. Electronic address:

Social anxiety is a common problem that usually emerges at puberty, during which great developmental changes occur both in the brain and mental state. However, little is known about the influence of social anxiety on adolescents' brain and behavior. The present study investigated the neural basis of social anxiety using voxel-based morphometry (VBM) and functional connectivity analysis. Then we investigated whether social anxiety is associated with attention bias. Furthermore, we investigated the neural basis of this association. Finally, longitudinal data was used to test if these biomarkers could predict social anxiety. The results indicated that social anxiety is positively associated with the grey matter volume (GMV) of orbital-frontal cortex (OFC), and the functional connectivity (FC) of OFC-amygdala. Mediation analysis revealed that the relationship between social anxiety and attention avoidance is partly mediated by the FC of OFC-amygdala. Finally, the present study demonstrated a close relationship between FC of the OFC-amygdala, the GMV of the OFC and the individual's social anxiety one year later. The present study suggested the aberrant structure of OFC and its connectivity with amygdala as the neural underpinning of social anxiety, which might serve as a compensatory mechanism to decrease attention avoidance and promote effective emotion regulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dcn.2020.100804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301179PMC
August 2020

Silage Fermentation: A Potential Biological Approach for the Long-Term Preservation and Recycling of Polyphenols and Terpenes in Globe Artichoke ( L.) By-Products.

Molecules 2020 Jul 21;25(14). Epub 2020 Jul 21.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

An economic and effective method for storage is necessary to make full use of the nature of active components in artichoke by-products and ease environmental pressure. In this paper, the potential of silage fermentation for the preservation and recycling of polyphenols and terpenes in artichoke by-products is evaluated. The silage of artichoke by-products is characterized by lactic acid bacteria fermentation. Silage distinctly increases the abundance of lactic acid bacteria in artichoke by-products, such as , , , and , and greatly increases the abundance of . The improvement of the microorgan structure and composition is of great significance for the quality of artichoke by-products. Polyphenols in the stems and leaves of artichokes are preserved well in silage. Among the 18 polyphenol compounds detected by high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (HPLC-QqQ-MS/MS), the contents of 11 phenolic acids and four flavonoids increased significantly. For terpenes detected by gas chromatography-mass spectrometry (GC-MS), the contents of four pentacyclic triterpenoids increased significantly, while two sterols were kept stable in the silage process. Silage is a potential biotechnology for the long-term preservation of bioactive components, such as polyphenols and terpenes in artichoke by-products, and the results provide a scientific basis for the efficient utilization of by-products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25143302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397312PMC
July 2020

Binocular Imbalance in Amblyopia Depends on Spatial Frequency in Binocular Combination.

Invest Ophthalmol Vis Sci 2020 07;61(8)

,.

Purpose: To assess the role of spatial frequency on binocular imbalance in binocular combination in adults with amblyopia.

Methods: Ten amblyopes (23 ± 4.9 [SD] years old; one deprivation, two mixed, seven anisometropia patients) and 10 age-matched normal adults (23 ± 2.3 years old) participated. The interocular contrast ratio (fellow eye/amblyopic eye, i.e., the balance point [BP]) that resulted in an equal contribution of both eyes in binocular combination was measured using a binocular orientation combination task at 0.5, 1, 2, and 4 cycles per degree (c/d). The extent of binocular imbalance was quantified as the absolute value of the BP on log scale (i.e., |logBP|).

Results: When the base contrast of the amblyopic eye was set at 100% (Experiment 1), the |logBP| was found to be significantly affected by stimulus spatial frequency (F(1.44, 26.01) = 51.6, P < 0.001, \({\rm{\eta }}_g^2\)= 0.40) and group (F(1, 18) = 66.97, P < 0.001, \({\rm{\eta }}_g^2\) = 0.74), the interaction between spatial frequency and group was also significant (F(1.44, 26.01) = 38.12, P < 0.001, \({\rm{\eta }}_g^2\)= 0.33). Such spatial frequency-dependent binocular imbalance remained present, even when the base contrast of the amblyopic eye was set at equal suprathreshold contrast levels across spatial frequencies (Experiment 2).

Conclusions: Binocular balance was more disrupted at higher spatial frequencies in binocular combination in amblyopia. This imbalance might not originate solely from the amblyopic eye's deficit in contrast sensitivity but is likely to be related to the difference in contrast sensitivity between the eyes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.61.8.7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425706PMC
July 2020

Measuring Aptamer Folding Energy Using a Molecular Clamp.

J Am Chem Soc 2020 07 22;142(27):11743-11749. Epub 2020 Jun 22.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.

Folding energy (Δ) offers a useful metric for characterizing the stability and function of aptamers. However, experimentally measuring the folding energy is challenging, and there is currently no general technique to measure this parameter directly. In this work, we present a simple approach for measuring aptamer folding energy. First, the aptamer is stretched under equilibrium conditions with a double-stranded DNA "molecular clamp" that is coupled to the aptamer ends. We then measure the total internal energy of stressed DNA molecules using time-lapse gel electrophoresis and compare the folding and unfolding behavior of molecular clamp-stressed molecules that incorporate either the aptamer or unstructured random single-stranded DNA in order to derive the aptamer folding energy. Using this approach, we measured a folding energy of 10.40 kJ/mol for the HD22 thrombin aptamer, which is consistent with other predictions and estimates. We also analyzed a simple hairpin structure, generating a folding energy result of 9.05 kJ/mol, consistent with the value predicted by computational models (9.24 kJ/mol). We believe our strategy offers an accessible and generalizable approach for obtaining such measurements with virtually any aptamer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c01570DOI Listing
July 2020

Association between pyrethroid pesticide exposure and hearing loss in adolescents.

Environ Res 2020 08 8;187:109640. Epub 2020 May 8.

Department of Traditional Medicine, The People's Hospital of Cangnan, Wenzhou Medical University, No.2288 Yucang Road, Cangnan County, Zhejiang, 325800, China. Electronic address:

Background: Hearing loss in adolescents is a serious public health problem with a high prevalence. Pyrethroids are one of the most widely applied insecticides that have been linked to neurotoxicity. However, there is no study about the effect of pyrethroid insecticide exposure on the auditory system in the general population.

Objective: To investigate the association between pyrethroid pesticide exposure and hearing loss in adolescents in the United States.

Methods: A total of 720 adolescents aged 12-19 years who participated in the National Health and Nutrition Examination Survey (NHANES, 2007-2010) were considered. 3-phenoxybenzoic acid (3-PBA), a urinary metabolite, was applied as a biomarker to assess pyrethroid exposure. Hearing loss in adolescents was defined as a pure-tone average (PTA) > 15 dB in either ear. Multivariate linear and logistic regression analyses were conducted to examine the associations of urinary 3-PBA with PTA hearing thresholds and risk of hearing loss, respectively.

Results: The weighted geometric mean of 3-PBA levels in urine was 0.32 μg/g creatinine, and 7.62% of adolescents had hearing loss. After adjusting for age, sex, race/ethnicity, BMI, serum cotinine, annual family income and exposure to loud noise/music, linear regression analyses found that Ln-transformed 3-PBA was positively correlated with increase of hearing thresholds in either left (β = 0.61, 95% CI: 0.20-1.01) or right ear (β = 0.52, 95% CI: 0.16-0.89). Logistic regression analyses showed that adjusted odds ratio (OR) for hearing loss in adolescents with the highest tertile (≥0.52 μg/g creatinine) of 3-PBA were 3.12 (95% CI: 1.42-6.83) compared with the lowest tertile (<0.18 μg/g creatinine), with significant linear trends across tertiles.

Conclusion: Pyrethroid pesticide exposure was positively associated with hearing loss in U.S. adolescents. This study provides new evidence for the association between pyrethroid exposure and auditory function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2020.109640DOI Listing
August 2020

Perioperative Dexmedetomidine Fails to Improve Postoperative Analgesic Consumption and Postoperative Recovery in Patients Undergoing Lateral Thoracotomy for Thoracic Esophageal Cancer: A Randomized, Double-Blind, Placebo-Controlled Trial.

Pain Res Manag 2020 6;2020:4145893. Epub 2020 May 6.

Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei City, Anhui Province, China.

Objectives: Dexmedetomidine is widely used as an adjunct to general anesthesia. In this study, we evaluated the effects of perioperative dexmedetomidine infusion on postoperative analgesia in patients undergoing lateral thoracotomy for thoracic esophageal cancer.

Methods: A total of 62 patients undergoing lateral thoracotomy for thoracic esophageal cancer were randomized to receive adjuvant therapy with either dexmedetomidine (0.5 g/kg intravenous bolus injection for 10 min before induction of anesthesia, followed by continuous infusion of 0.2-0.4 g/kg/h until the end of surgery, and 0.06 g/kg/h for 5 days after surgery) or equal volumes of saline. Acute postoperative pain was treated with patient-controlled intravenous sufentanil and flurbiprofen axetil. The primary outcomes of this study were the numbers of analgesic requirements in the first postoperative 72 h.

Results: Perioperative dexmedetomidine did not decrease the numbers of analgesic requirements in the first postoperative 72 h (dexmedetomidine group: 12.14 ± 4.76, saline group: 10.89 ± 5.66; =0.367). Likewise, the groups did not differ with respect to total postoperative analgesic requirements, postoperative pain, perioperative inflammation, blood cell count, incidence of adverse events, surgical recovery (assessed at postoperative days 2 and 5 using the surgical recovery scale), length of hospital stay, hospital cost, incidence of chronic pain, or quality of life. Notably, dexmedetomidine had beneficial effects on decreasing intraoperative opioid consumption and improving postoperative sleep quality. . Perioperative dexmedetomidine has limited analgesic benefits in lateral thoracotomy for esophageal cancer when added to an opioid-based multimodal anesthetic regimen but can reduce opioid consumption.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/4145893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229565PMC
October 2020

Prevalence of Opportunistic Pathogens and Diversity of Microbial Communities in the Water System of a Pulmonary Hospital.

Biomed Environ Sci 2020 Apr;33(4):248-259

State Key Laboratory for Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

Objective: Our objective was to investigate the occurrence of opportunistic pathogens and characterize the bacterial community structures in the water system of a pulmonary hospital.

Methods: The water samples were collected from automatic and manual faucets in the consulting room, treatment room, dressing room, respiratory ward, and other non-medical rooms in three buildings of the hospital. Quantitative polymerase chain reaction was used to quantify the load of several waterborne opportunistic pathogens and related microorganisms, including spp., spp., and . Illumina sequencing targeting 16S rRNA genes was performed to profile bacterial communities.

Results: The occurrence rates of spp., spp., and were 100%, 100%, and 76%, respectively in all samples. Higher occurrence rates of were observed in the outpatient service building (building 1, 91.7%) and respiration department and wards (building 2, 80%) than in the office building (building 3), where no was found. were more abundant in automatic faucets (average 2.21 × 10 gene copies/L) than in manual faucets (average 1.03 × 10 gene copies/mL) ( < 0.01). , , , , , and were the dominant bacterial phyla. Disinfectant residuals, nitrate, and temperature were found to be the key environmental factors driving microbial community structure shifts in water systems.

Conclusion: This study revealed a high level of colonization of water faucets by opportunistic pathogens and provided insight into the characteristics of microbial communities in a hospital water system and approaches to reduce risks of microbial contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3967/bes2020.034DOI Listing
April 2020