Publications by authors named "Yu Ma"

656 Publications

RP11-874J12.4, a novel lncRNA, confers chemoresistance in human gastric cancer cells by sponging miR-3972 and upregulating SSR2 expression.

Am J Transl Res 2021 15;13(6):5892-5910. Epub 2021 Jun 15.

Department 2 of General Surgery, Shaanxi Provincial People's Hospital Xi'an 710068, China.

Increasing evidence has revealed the contributions of long noncoding RNAs (lncRNAs) in the modulation of drug resistance in gastric cancer. In the present study, we explored the role of a novel lncRNA, RP11-874J12.4, in regulating chemoresistance in gastric cancer and determined the underlying molecular mechanisms. We observed that compared with normal controls, human gastric cancer tissues and cell lines, including MKN-45 and AGS cells, expressed higher RP11-874J12.4 levels. RP11-874J12.4 knockdown sensitized MKN-45 and AGS cells to docetaxel and cisplatin in terms of cell viability and apoptosis rate. In addition, RP11-874J12.4 was found to be a competing endogenous RNA that sponged microRNA (miR)-3972, which showed significantly reduced expression in human gastric cancer tissues and cell lines. Furthermore, signal sequence receptor subunit 2 (SSR2) was identified as a downstream target of miR-3972, and the miR-3972/SSR2 axis was found to regulate chemoresistance in MKN-45 and AGS cells. SSR2 downregulation further sensitized gastric cancer cells with RP11-874J12.4 knockdown to chemotherapeutic drugs via enhanced apoptosis, which was evidenced by significantly upregulated expressions of cleaved caspase-3, cleaved caspase-9, and Bax and downregulated expression of Bcl-2. Furthermore, RP11-874J12.4 knockdown markedly inhibited the growth of xenograft MKN-45 cells in nude mice, which was associated with an increased expression of miR-3972 and decreased expression of SSR2 in tumors. Therefore, the RP11-874J12.4/miR-3972/SSR2 axis plays important roles in the regulation of chemoresistance in MKN-45 and AGS cells and may serve as a target for the diagnosis and treatment of human gastric cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290636PMC
June 2021

A single nucleotide polymorphism electrochemical sensor based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probes.

Mikrochim Acta 2021 Jul 21;188(8):266. Epub 2021 Jul 21.

College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, People's Republic of China.

An ultrasensitive electrochemical sensor has been constructed for the detection of single nucleotide polymorphisms (SNPs) based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probe under enzyme-free conditions. Interestingly, the introduction of an auxiliary probe did not disturb the detection of SNP targets, but could bind more Cd-MOFs-74 signal elements to enhance the different pulse voltammetry electrochemical signal 2~3 times as compared to sensing system without auxiliary probe, which obviously improves the sensitivity of the proposed sensor. Experimental results taking p53 tumor suppressor gene as SNP model demonstrated that the proposed method can be employed to sensitively and selectively detect target p53 gene fragment with a linear response ranging from 0.01 to 30 pmol/L (detection limit of 6.3 fmol/L) under enzyme-free conditions. Utilizing this strategy, the ultrasensitive SNP electrochemical sensor is a promising tool for the determination  of SNPs in biomedicine. Graphical Abstract.
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http://dx.doi.org/10.1007/s00604-021-04924-9DOI Listing
July 2021

Preparation and characteristics analysis of an ecoenvironmental protection cyclic solidification dust-fixing agent extracted from waste shrimp shells to suppress dust in coal resource-based cities.

J Environ Manage 2021 Jul 13;296:113224. Epub 2021 Jul 13.

College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, China; State Key Laboratory of Mining Disaster Prevention and Control Co-founded By Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, China.

There are a lot of coal cinder and dust in the development of coal resource-based cities. Improper handling will cause dust flying, which has a great impact on urban residents and the environment. The purpose of this research is to follow the principle of waste recycling, to use waste shrimp shells to extract chitosan as a raw material, to carry out graft polymerization with acrylic acid and polyvinyl alcohol-1788, and to carry out amidation crosslinking reactions with ethylenediamine tetraacetic acid to produce products. The results showed that the degree of deacetylation of chitosan reached 85% with the optimized extraction method, which greatly shortened the process cycle. Through orthogonal experiments and osmotic wetting experiments, the best preparation process was determined. The reaction process, structure of the product and adhesive structure of the solidified layer were analyzed by FTIR, XPS and SEM. The thermal stability of the product was analyzed by thermogravimetry. Relevant performance tests showed that the product had good adhesion with dust, the hardness of the solidified layer could reach 98 HA, and the dust fixation rate was 92.67% when the wind speed was 15-18 m/s. It has the advantage of circulating dust fixation, which improves the utilization rate of resources.
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http://dx.doi.org/10.1016/j.jenvman.2021.113224DOI Listing
July 2021

The identification of as the vector of mulberry crinkle leaf virus and the infectivity of infectious clones in mulberry.

Phytopathology 2021 Jul 14. Epub 2021 Jul 14.

Jiangsu University of Science and Technology, 12676, College of Biotechnology, Zhenjiang, China;

Mulberry crinkle leaf virus (MCLV) is a novel geminivirus identified from mulberry. The pathogenicity and the natural vector of transmission have remained unknown for MCLV. Here, the infectious clones which consisted of the complete tandem dimeric genome of MCLV in a binary vector were constructed and agro-inoculated into mulberry seedlings. The results showed that the infectious clones of MCLV were systemically infectious to mulberry, but the infected mulberry plants did not show any virus-like symptoms. The natural transmission vectors of MCLV were also identified from possible vector insects occurring on the MCLV-infected mulberry plants. The vector ability of Matsumura was identified through inoculation assay. Three of 21 (14.3%) seedlings inoculated with collected from MCLV-infected mulberry plants grown naturally were detected to be MCLV-positive 50 days post-inoculation. These MCLV-positive mulberry plants did also not show any virus-like symptoms. Collectively, it is suggested that MCLV is infectious to mulberry plants, but MCLV alone does not induce symptoms. The leafhopper was for the first time determined experimentally to be a transmission vector of MCLV.
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http://dx.doi.org/10.1094/PHYTO-03-21-0094-RDOI Listing
July 2021

Geminiviruses encode additional small proteins with specific subcellular localizations and virulence function.

Nat Commun 2021 07 13;12(1):4278. Epub 2021 Jul 13.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Geminiviruses are plant viruses with limited coding capacity. Geminivirus-encoded proteins are traditionally identified by applying a 10-kDa arbitrary threshold; however, it is increasingly clear that small proteins play relevant roles in biological systems, which calls for the reconsideration of this criterion. Here, we show that geminiviral genomes contain additional ORFs. Using tomato yellow leaf curl virus, we demonstrate that some of these small ORFs are expressed during the infection, and that the encoded proteins display specific subcellular localizations. We prove that the largest of these additional ORFs, which we name V3, is required for full viral infection, and that the V3 protein localizes in the Golgi apparatus and functions as an RNA silencing suppressor. These results imply that the repertoire of geminiviral proteins can be expanded, and that getting a comprehensive overview of the molecular plant-geminivirus interactions will require the detailed study of small ORFs so far neglected.
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http://dx.doi.org/10.1038/s41467-021-24617-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277811PMC
July 2021

Polystyrene microplastics trigger hepatocyte apoptosis and abnormal glycolytic flux via ROS-driven calcium overload.

J Hazard Mater 2021 09 15;417:126025. Epub 2021 May 15.

Xiangya School of Public Health, Central South University, Changsha 410078, PR China. Electronic address:

Human health could be affected by the spread of microplastics in the food chain. Our previous research has indicated that microplastics accumulated in the liver and subsequently induce oxidative damage. However, the molecular events linking oxidative stress to calcium ion (Ca) signaling during microplastics stress remains elusive. The present research demonstrated that up-regulation of Orai 1 and stromal interaction molecule 1 (Stim1) expression participated in the microplastics-triggered Ca overload, accompanied with the down-regulation of arcoplasmic reticulum Ca ATPase (SERCA). However, when the protein expression of Stim1/SERCA is restored, microplastics-induced Ca overload is ameliorated. Further analysis revealed that inhibiting the microplastics-induced Ca overload was integral to prevent hepatocyte apoptosis and S phase arrest in the L02 hepatocyte. Simultaneously, we observed that inhibiting microplastics-evoked reactive oxygen species (ROS) could alleviate Ca overload via reversing expression of store-operated Ca channels (SOCs). These changes were accompanied by restoration of glycolytic flux, likely due to the regulation of AMP-activated protein kinase (AMPK)-PGC-1α signaling. Our findings highlight the role of SOCs at microplastics-evoked ROS in Ca overload, and its a crucial step in triggering hepatocyte death. Collectively, this study reveals a regulatory paradigm that links ROS with AMPK and Ca signaling in microplastics-triggered hepatotoxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126025DOI Listing
September 2021

Microwave ablation versus radiofrequency ablation for primary hyperparathyroidism: a multicenter retrospective study.

Int J Hyperthermia 2021 ;38(1):1023-1030

Department of Interventional Medicine, China-Japan Friendship Hospital, Beijing, China.

Objective: To compare the clinical outcomes of microwave ablation (MWA) and radiofrequency ablation (RFA) in the treatment of primary hyperparathyroidism (pHPT).

Method: This retrospective study included 104 pHPT patients treated by MWA or RFA between January 2015 and March 2020 in four centers. The clinical outcomes including effectiveness and complications were compared between the two groups. Ablation cure was defined as the reestablishment of normal values of serum calcium and intact parathyroid hormone (iPTH) at least more than 6 months. Clinical cure was defined as the reestablishment of normal values of serum calcium and iPTH throughout the entire follow-up period.

Results: A total of 77 patients underwent MWA (mean age, 55.5 ± 16.4 years) and 27 underwent RFA (mean age, 58.9 ± 15.6 years). During the follow-up (median, 18.7 months in the MWA group; 12 months in the RFA group), no difference was observed between ablation cure rates (88.3% vs. 88.9%,  = 1.000), clinical cure rates (87.0% vs. 82.3%,  = .880), recurrent pHPT (5.2% vs. 3.7%,  = .447), persistent pHPT (11.7% vs. 11.1%,  = 1.000) and complication rate (9.1% vs. 3.7%,  = .677). A maximum diameter less than 0.7 cm was an independent prognostic factor of uncured pHPT in ablation (hazard ratio, 0.1; 95% confidence interval: 0.02, 0.54;  = .007). Major complication - voice change encountered in five patients (6.5%) in the MWA group and in one patient (3.7%) in the RFA group.

Conclusion: Both RFA and MWA are safe and effective techniques for patients with pHPT, with comparable clinical outcomes.
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http://dx.doi.org/10.1080/02656736.2021.1945689DOI Listing
January 2021

Clusterin inhibits Cr(VI)-induced apoptosis via enhancing mitochondrial biogenesis through AKT-associated STAT3 activation in L02 hepatocytes.

Ecotoxicol Environ Saf 2021 Sep 25;221:112447. Epub 2021 Jun 25.

Xiangya School of Public Health, Central South University, Changsha 410078, PR China. Electronic address:

Improper treatment of a large amount of industrial waste makes hexavalent chromium [Cr(VI)] seriously pollute the atmosphere, soil and water, and enter the food chain, seriously affecting the health of workers and local residents. We previously proved that Clusterin (CLU) can inhibit the apoptosis of L02 hepatocytes induced by Cr(VI) through mitochondrial pathway, but the associated molecular mechanism has not been further studied. Mitochondrial biogenesis is an important step in mitochondrial damage repair, but the mechanism of mitochondrial biogenesis in Cr(VI)-induced liver toxicity is still unclear. We demonstrated in the present study that Cr(VI) triggered mitochondrial biogenesis dysfunction-associated apoptosis, and CLU delayed Cr(VI)-induced apoptosis by enhancing mitochondrial biogenesis. Signal transducer and activator of transcription 3 (STAT3) was down-regulated in Cr(VI)-induced apoptosis, and CLU may regulate STAT3 via protein kinase B (PKB/AKT) in Cr(VI)-exposed hepatocytes. We used the STAT3 inhibitor C188-9 and the AKT inhibitor Uprosertib to eliminate the anti-apoptotic effect of CLU, and found that CLU inhibited Cr(VI)-induced apoptosis by up-regulating AKT/STAT3 signal. Based on the fact that both AKT and STAT3 are closely related to mitochondrial biogenesis and mitochondrial pathway-associated apoptosis, this study is the first time to link CLU, STAT3, AKT and mitochondrial biogenesis function after Cr(VI) exposure, to further enrich the experimental basis of Cr(VI)-induced hepatotoxicity, clarify the molecular mechanism of CLU helping cells to escape apoptosis, and also suggest that new ways can be sought to prevent and treat Cr(VI)-induced hepatotoxicity by regulating mitochondrial biosynthesis.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112447DOI Listing
September 2021

serves as a prognostic factor of breast cancer and promotes cell proliferation through activating AKT/mTORC1 signaling pathway.

Ann Transl Med 2021 May;9(10):892

Department of Oncology, School of Medicine, Southeast University, Nanjing, China.

Background: The purpose of our research was to determine if the clinical, pathological, and prognostic functions of are the same as those of other molecular breast cancer (BC) subgroups.

Methods: We used the Oncomine and The Cancer Genome Atlas (TCGA) online databases to examine the expression and genetic changes of in BC tissues. Immunohistochemical analysis was used to validate the protein expression in subtypes of BC, while Kaplan-Meier figures and log-rank tests were used to evaluate the prognostic relevance of . Uni- and multivariate Cox regression models were adapted to analyze hazard ratios (HRs) and the independent prognostic factors. We analyzed the alterations of different malignancies of luminal cells by up-regulation of in human luminal cell lines MCF-7. was overexpressed in luminal cells, and then the AKT, mTOR, and p70-S6K phosphorylation and expression were analyzed by western blot analysis and real-time quantitative polymerase chain reaction (qPCR).

Results: Our results suggested that was overexpressed in BC cell lines and in patients' tissues. Elevated messenger RNA (mRNA) and protein expression was correlated to a worse clinical prognosis (P<0.001) in luminal subtypes of BC. The multivariate analysis suggested that high level of expression could be an independent prognostic factor for decreased overall survival (OS). The study also demonstrated that overexpression increased proliferation of MCF-7 cells by reducing the cell cycle arrest in G1 phase. Our mechanistic study further indicates that enhances the proliferation of the MCF-7 cell by activation of AKT/mTORC1 pathway through phosphorylation.

Conclusions: Our study demonstrated that may have a vital function in the biology of BC cells, indicating that is a potential prognostic biomarker and may be a valuable therapeutic target in BC patients.
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http://dx.doi.org/10.21037/atm-21-2247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184433PMC
May 2021

Human papillomavirus vaccination coverage and knowledge, perceptions and influencing factors among university students in Guangzhou, China.

Hum Vaccin Immunother 2021 Jun 22:1-10. Epub 2021 Jun 22.

Disease Control and Prevention Department, Guangzhou Health Committee, Guangzhou, Guangdong Province, P.R. China.

HPV vaccines were administered in mainland China from July 2017 at a gradual rate. We aimed to assess the vaccination rate and vaccination influencing factors among college students in mainland China. From October to December 2018, we conducted face-to-face questionnaires including 5 sections and 22 questions to collect demographic information, HPV infection and transmission knowledge, HPV vaccine knowledge and attitudes among college students in Guangzhou, China. HPV vaccine vaccination status and cervical screening behaviors were self-reported. Knowledge and attitudes differences between the vaccinate and non-vaccinate groups were analyzed using univariable logistic regression. Vaccination-related influencing factors were estimated using multivariable logistic regression. 5307 of 5414 valid questionnaires were collected. The self-reported cervical screening rate and HPV vaccine coverage were 11.82% (9.03%-14.61%) and 3.09% (2.62%-3.56%). In total, 55.57% of the participants were hesitant about vaccination. Urban residence (OR = 2.1, 95%CI: 1.4-3.3), high monthly consumption (OR = 2.6, 95%CI: 1.9-3.6), awareness of vaccination adaptive population (OR = 3.1, 95%CI: 1.9-5.0), awareness of infection-related risk factors (OR = 2.5, 95%CI: 1.1-5.7), and awareness of HPV vaccine effectiveness (OR = 3.2, 95%CI: 2.0-5.2) were significant in multivariable logistic regression. HPV vaccine coverage is quite low among college students in China Guangzhou. Economic affordability, awareness of HPV infection, and belief in the effectiveness of HPV vaccine are influencing factors for vaccination. In the future, establishing a national financial subsidy and strengthening health education is needed to increase the vaccination rate in China.
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http://dx.doi.org/10.1080/21645515.2021.1927411DOI Listing
June 2021

Opening MXene Ion Transport Channels by Intercalating PANI Nanoparticles from the Self-Assembly Approach for High Volumetric and Areal Energy Density Supercapacitors.

ACS Appl Mater Interfaces 2021 Jul 22;13(26):30633-30642. Epub 2021 Jun 22.

College of Chemistry and Materials Engineering, Xinxiang University, Xinxiang 453003, P. R. China.

Two-dimensional (2D) MXene materials have attracted great attention as advanced energy storage devices. A TiC MXene film can be used as a high-performance electrode material for flexible supercapacitors owing to its high specific capacitance, excellent conductivity, and remarkable flexibility. Unfortunately, self-stacking of MXene nanosheets makes them hard to balance the volumetric and areal capacitance performance. Herein, high conductive polyaniline nanoparticles (PANI NPs, ∼10 nm) are proposed as intercalators to regulate the MXene nanosheet interlayer by the self-assembly method. Interlayered PANI NPs not only restrain MXene self-stacking but also enable more ion transport routes, and conductive PANI NPs filled in MXene interlayer are in the form of nanoparticles that can build interconnected conductive channels. Meanwhile, PANI NPs slightly changes the thickness of the MX/PANI NPs hybrid film, thus bringing a high volumetric capacitance. As a result, the freestanding MX/PANI NPs-10% electrode displays an excellent areal capacitance of 1885 mF cm (377 F g), meanwhile maintains a high volumetric capacitance of 873 F cm even when the load of MXene reaches 5 mg cm. Moreover, the symmetric supercapacitor assembled by MX/PANI NPs hybrid film demonstrates high areal energy density (90.3 μWh cm) and volumetric energy density (20.9 Wh L) compared to MXene-based symmetric supercapacitors reported in the literature. This rational design balancing areal and volumetric energy densities provides another approach for solving the inherent problems of MXene and further exploiting MXene materials toward application in advanced energy storage devices.
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http://dx.doi.org/10.1021/acsami.1c06934DOI Listing
July 2021

Ultrasound-guided thermal ablation for papillary thyroid microcarcinoma: a multicenter retrospective study.

Int J Hyperthermia 2021 ;38(1):916-922

Department of Ultrasound, the First Affiliated Hospital of Dalian Medical University, Dalian, China.

Purpose: Ultrasound-guided thermal ablation (including microwave ablation [MWA] and radiofrequency ablation [RFA]) has emerged as a remarkable technology for the treatment of benign and malignant diseases. The objective of this multicenter study was to assess the efficacy and safety of thermal ablation in a large cohort of patients with papillary thyroid microcarcinoma (PTMC).

Materials And Methods: Retrospective study of 725 patients who underwent MWA/RFA at 11 centers between March 2015 and March 2020. The mean age of patients was 46 ± 11 years (range, 22-81); the mean follow-up time was 21 ± 13 months (range, 6-60). Changes in size of tumor, the rates of tumor disappearance, disease progression, and complications were assessed.

Results: From 6 months post-ablation, the size of tumors was significantly reduced compared with those recorded pre-ablation ( < 0.001 for all). Five hundred and fifteen (71.0%) PTMCs had completely disappeared as assessed by ultrasound examination. Six (0.8%) patients developed disease progression post-ablation; of these, 5 (0.7%) patients developed new PTMCs, while one (0.1%) patient developed cervical lymph node metastasis. Nineteen (2.6%) patients developed complications post-ablation; of these 14 (1.9%) patients developed voice hoarseness, 4 (0.6%) developed hematoma, and one (0.1%) patient developed cough.

Conclusions: Ultrasound-guided thermal ablation represents an effective and safe treatment for patients with PTMC besides active surveillance and surgery.
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http://dx.doi.org/10.1080/02656736.2021.1936218DOI Listing
July 2021

Bacterial dynamics and functions for gaseous emissions and humification in response to aeration intensities during kitchen waste composting.

Bioresour Technol 2021 Oct 3;337:125369. Epub 2021 Jun 3.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

This study revealed bacteria dynamics and functions for gaseous emissions and humification during kitchen waste composting under different aeration intensities (i.e. 0.24, 0.36, and 0.48 L kg DM min) using high-throughput sequencing with Functional Annotation of Prokaryotic Taxa. Results show that aeration increase restrained bacteria (e.g. Lactobacillus and Acinetobacter) for fermentation, nitrate reduction, and sulphur/sulphate respiration, but enriched thermophilic bacteria (e.g. Thermomonospora and Thermobifida) for aerobic chemohetertrophy, xylanolysis, cellulolysis, and methylotrophy. Thus, high aeration intensity (i.e. above 0.36 L kg DM min) effectively alleviated the emission of greenhouse gases and hydrogen sulphide, and meanwhile facilitated the production of humus precursors and ammonia. Nevertheless, humification was limited by the conclusion of composting under high aeration conditions due to the consumption of humus precursors for bacterial activity. Thus, aeration intensity should be regulated at different stages indicated by temperature to balance gaseous emissions and humification during kitchen waste composting.
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http://dx.doi.org/10.1016/j.biortech.2021.125369DOI Listing
October 2021

Development and application of the Faba_bean_130K targeted next-generation sequencing SNP genotyping platform based on transcriptome sequencing.

Theor Appl Genet 2021 Jun 12. Epub 2021 Jun 12.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Key Message: Large-scale faba bean transcriptome data are available, and the first genotyping platform based on liquid-phase probe targeted capture technology was developed for genetic and molecular breeding studies. Faba bean (Vicia faba L., 2n = 12) is an important food legume crop that is widely grown for multiple uses worldwide. However, no reference genome is currently available due to its very large genome size (approximately 13 Gb) and limited single nucleotide polymorphism (SNP) markers as well as highly efficient genotyping tools have been reported for faba bean. In this study, 16.7 billion clean reads were obtained from transcriptome libraries of flowers and leaves of 102 global faba bean accessions. A total of 243,120 unigenes were de novo assembled and functionally annotated. Moreover, a total of 1,579,411 SNPs were identified and further filtered according to a selection pipeline to develop a high-throughput, flexible, low-cost Faba_bean_130K targeted next-generation sequencing (TNGS) genotyping platform. A set of 69 Chinese faba bean accessions were genotyped with the TNGS genotyping platform, and the average mapping rate of captured reads to reference transcripts was 93.14%, of which 53.23% were located in the targeted regions. The TNGS genotyping results were validated by Sanger sequencing and the average consistency rate reached 93.6%. Comprehensive population genetic analysis was performed on the 69 Chinese faba bean accessions and identified four genetic subgroups correlated with the geographic distribution. This study provides valuable genomic resources and a reliable genotyping tool that could be implemented in genetic and molecular breeding studies to accelerate new cultivar development and improvement in faba bean.
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http://dx.doi.org/10.1007/s00122-021-03885-0DOI Listing
June 2021

A robust discriminative multi-atlas label fusion method for hippocampus segmentation from MR image.

Comput Methods Programs Biomed 2021 May 25;208:106197. Epub 2021 May 25.

School of Computer Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China; Shaanxi Provincial Key Lab. of Speech and Image Information Processing (SAIIP), School of Computer Science, Northwestern Polytechnical University, Xi'an, China; National Engineering Laboratory for Air-Sea-Earth-Sea Integrated Big Data Application Technology, China.

Accurate and automatic segmentation of the hippocampus plays a vital role in the diagnosis and treatment of nervous system diseases. However, due to the anatomical variability of different subjects, the registered atlas images are not always perfectly aligned with the target image. This makes the segmentation of the hippocampus still face great challenges. In this paper, we propose a robust discriminative label fusion method under the multi-atlas framework. It is a patch embedding label fusion method based on conditional random field (CRF) model that integrates the metric learning and the graph cuts by an integrated formulation. Unlike most current label fusion methods with fixed (non-learning) distance metrics, a novel distance metric learning is presented to enhance discriminative observation and embed it into the unary potential function. In particular, Bayesian inference is utilized to extend a classic distance metric learning, in which large margin constraints are instead of pairwise constraints to obtain a more robust distance metric. And the pairwise homogeneity is fully considered in the spatial prior term based on classification labels and voxel intensity. The resulting integrated formulation is globally minimized by the efficient graph cuts algorithm. Further, sparse patch based method is utilized to polish the obtained segmentation results in label space. The proposed method is evaluated on IABA dataset and ADNI dataset for hippocampus segmentation. The Dice scores achieved by our method are 87.2%, 87.8%, 88.2% and 88.9% on left and right hippocampus on both two datasets, while the best Dice scores obtained by other methods are 86.0%, 86.9%, 86.8% and 88.0% on IABA dataset and ADNI dataset respectively. Experiments show that our approach achieves higher accuracy than state-of-the-art methods. We hope the proposed model can be transferred to combine with other promising distance measurement algorithms.
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http://dx.doi.org/10.1016/j.cmpb.2021.106197DOI Listing
May 2021

Pain Management in People with Diabetes-Related Chronic Limb-Threatening Ischemia.

J Diabetes Res 2021 8;2021:6699292. Epub 2021 May 8.

Department of Rehabilitation, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.

Management of neuropathic pain in people with diabetes has been widely investigated. However, little attention was paid to address ischemic-related pain in patients with diabetes mellitus who suffered from chronic limb-threatening ischemia (CLTI), the end stage of lower extremity arterial disease (LEAD). Pain management has a tremendous influence on patients' quality of life and prognosis. Poor management of this type of pain owing to the lack of full understanding undermines patients' physical and mental quality of life, which often results in a grim prognosis, such as depression, myocardial infarction, lower limb amputation, and even mortality. In the present article, we review the current strategy in the pain management of diabetes-related CLTI. The endovascular therapy, pharmacological therapies, and other optional methods could be selected following comprehensive assessments to mitigate ischemic-related pain, in line with our current clinical practice. It is very important for clinicians and patients to strengthen the understanding and build intervention strategy in ischemic pain management and possible adverse consequence.
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http://dx.doi.org/10.1155/2021/6699292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128546PMC
May 2021

Differential Metabolites in Chinese Autistic Children: A Multi-Center Study Based on Urinary H-NMR Metabolomics Analysis.

Front Psychiatry 2021 11;12:624767. Epub 2021 May 11.

Department of Neurology, Children's Hospital of Fudan University, Shanghai, China.

Autism spectrum disorder (ASD) is a group of early-onset neurodevelopmental disorders. However, there is no valuable biomarker for the early diagnosis of ASD. Our large-scale and multi-center study aims to identify metabolic variations between ASD and healthy children and to investigate differential metabolites and associated pathogenic mechanisms. One hundred and seventeen autistic children and 119 healthy children were recruited from research centers of 7 cities. Urine samples were assayed by H-NMR metabolomics analysis to detect metabolic variations. Multivariate statistical analysis, including principal component analysis (PCA), and orthogonal projection to latent structure discriminant analysis (OPLS-DA), as well as univariate analysis were used to assess differential metabolites between the ASD and control groups. The differential metabolites were further analyzed by receiver operating characteristics (ROC) curve analysis and metabolic pathways analysis. Compared with the control group, the ASD group showed higher levels of glycine, guanidinoacetic acid, creatine, hydroxyphenylacetylglycine, phenylacetylglycine, and formate and lower levels of 3-aminoisobutanoic acid, alanine, taurine, creatinine, hypoxanthine, and N-methylnicotinamide. ROC curve showed relatively significant diagnostic values for hypoxanthine [area under the curve (AUC) = 0.657, 95% CI 0.588 to 0.726], creatinine (AUC = 0.639, 95% CI 0.569 to 0.709), creatine (AUC = 0.623, 95% CI 0.552 to 0.694), N-methylnicotinamide (AUC = 0.595, 95% CI 0.523 to 0.668), and guanidinoacetic acid (AUC = 0.574, 95% CI 0.501 to 0.647) in the ASD group. Combining the metabolites creatine, creatinine and hypoxanthine, the AUC of the ROC curve reached 0.720 (95% CI 0.659 to 0.777). Significantly altered metabolite pathways associated with differential metabolites were glycine, serine and threonine metabolism, arginine and proline metabolism, and taurine and hypotaurine metabolism. Urinary amino acid metabolites were significantly altered in children with ASD. Amino acid metabolic pathways might play important roles in the pathogenic mechanisms of ASD.
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http://dx.doi.org/10.3389/fpsyt.2021.624767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144639PMC
May 2021

NF1 mutation drives neuronal activity-dependent initiation of optic glioma.

Nature 2021 06 26;594(7862):277-282. Epub 2021 May 26.

Department of Neurology, Washington University School of Medicine, St Louis, MO, USA.

Neurons have recently emerged as essential cellular constituents of the tumour microenvironment, and their activity has been shown to increase the growth of a diverse number of solid tumours. Although the role of neurons in tumour progression has previously been demonstrated, the importance of neuronal activity to tumour initiation is less clear-particularly in the setting of cancer predisposition syndromes. Fifteen per cent of individuals with the neurofibromatosis 1 (NF1) cancer predisposition syndrome (in which tumours arise in close association with nerves) develop low-grade neoplasms of the optic pathway (known as optic pathway gliomas (OPGs)) during early childhood, raising  the possibility that postnatal light-induced activity of the optic nerve drives tumour initiation. Here we use an authenticated mouse model of OPG driven by mutations in the neurofibromatosis 1 tumour suppressor gene (Nf1) to demonstrate that stimulation of optic nerve activity increases optic glioma growth, and that decreasing visual experience via light deprivation prevents tumour formation and maintenance. We show that the initiation of Nf1-driven OPGs (Nf1-OPGs) depends on visual experience during a developmental period in which Nf1-mutant mice are susceptible to tumorigenesis. Germline Nf1 mutation in retinal neurons results in aberrantly increased shedding of neuroligin 3 (NLGN3) within the optic nerve in response to retinal neuronal activity. Moreover, genetic Nlgn3 loss or pharmacological inhibition of NLGN3 shedding blocks the formation and progression of Nf1-OPGs. Collectively, our studies establish an obligate role for neuronal activity in the development of some types of brain tumours, elucidate a therapeutic strategy to reduce OPG incidence or mitigate tumour progression, and underscore the role of Nf1mutation-mediated dysregulation of neuronal signalling pathways in mouse models of the NF1 cancer predisposition syndrome.
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http://dx.doi.org/10.1038/s41586-021-03580-6DOI Listing
June 2021

Analysis of unigenes involved in lateral root development in Bupleurum chinense and B. scorzonerifolium.

Planta 2021 May 26;253(6):128. Epub 2021 May 26.

Laboratory of Medicinal Plant Cultivation, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.

Main Conclusion: We identified IAA13 negatively associated with lateral root number by comparing the differential expressed genes between Bupleurum chinense and B. scorzonerifolium. Dried roots of the genus Bupleurum L. are used as a herbal medicine for diseases in Asia. Bupleurum chinense has a greater number of lateral roots than B. scorzonerifolium, but the genetic mechanisms for such differences are largely unknown. We (a) compared the transcriptome profiles of the two species and (b) identified a subset of candidate genes involved in auxin signal transduction and explored their functions in lateral root development. By isoform sequencing (Iso-Seq) analyses of the whole plant, more unigenes were found in B. scorzonerifolium (118,868) than in B. chinense (93,485). Given the overarching role of indole-3-acetic acid (IAA) as one of the major regulators of lateral root development, we identified 539 unigenes associated with auxin signal transduction. Fourteen and 44 unigenes in the pathway were differentially expressed in B. chinense and B. scorzonerifolium, respectively, and 3 unigenes (LAX2, LAX4, and IAA13) were expressed in both species. The number of lateral root primordia increased after exogenous auxin application at 8 h and 12 h in B. scorzonerifolium and B. chinense, respectively. Since overexpression of IAA13 in Arabidopsis reduced the number of lateral roots, we hypothesized that IAA13 is involved in the reduction of the number of lateral roots in B. scorzonerifolium.
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http://dx.doi.org/10.1007/s00425-021-03644-xDOI Listing
May 2021

Role of Clusterin/NF-κB in the secretion of senescence-associated secretory phenotype in Cr(VI)-induced premature senescent L-02 hepatocytes.

Ecotoxicol Environ Saf 2021 Aug 18;219:112343. Epub 2021 May 18.

Xiangya School of Public Health, Central South University, Changsha 410078, PR China. Electronic address:

Hexavalent chromium [Cr(VI)] and its compounds have caused serious environmental pollution and health damage. Senescent cells can actively change the surrounding environment by secreting some factors, which are called senescence associated secretory phenotype (SASP). Our previous work has confirmed that premature senescent hepatocytes induced by Cr(VI) expressed high level of Clusterin (CLU) and secrete interleukin-6 (IL-6) and IL-8. CLU is involved in the regulation of tumor development and drug resistance, but whether CLU regulates SASP components and participates in Cr(VI)-induced malignant transformation is unclear. In this study we demonstrated that Cr(VI) induced the secretion of tumor promoting components of SASP such as IL-6, IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF) in senescent L-02 hepatocytes, while the levels of the anti-tumor components of SASP such as chemokine (c-x-c motif) ligand-1 (CXCL-1) and monocyte chemoattractant protein-1 (MCP-1) were not altered. CLU shRNA interference significantly reduced the levels of IL-6, IL-8, and GM-CSF in the culture medium of senescent cells, suggesting CLU may regulate SASP. The NF-κB inhibitor PDTC significantly alleviated Cr(VI)-induced increase of IL-6, IL-8, and GM-CSF, confirming that NF-κB can regulate the tumor promoting components of SASP. CLU shRNA interference aggravated the inhibitory effect of PDTC on SASP secretion, indicating that CLU regulated the secretion of SASP in Cr(VI)-induced senescent hepatocytes through the NF-κB signaling. We speculated that SASP secreted by Cr(VI)-induced premature senescent hepatocytes was tightly related to the carcinogenic effect of Cr(VI). Therefore, elucidation of upstream regulatory mechanism of SASP is of great significance. In addition to further clarifying the carcinogenic mechanisms associated with Cr(VI), we could also seek out new targets for treatment of Cr(VI)-related cancer.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112343DOI Listing
August 2021

Iodine-DEM TACE conventional TACE in cirrhotic patients with hepatocellular carcinoma: a single center experiment.

J Gastrointest Oncol 2021 Apr;12(2):762-769

Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: To evaluate the safety and efficacy of transcatheter arterial chemoembolization (TACE) with iodine-doxorubicin-eluting gelatin microspheres (I-DEM TACE) compared with conventional TACE (cTACE) with polyvinyl alcohol foam (PVA) embolization microspheres.

Methods: A total of 22 patients diagnosed with hepatocellular carcinoma were equally divided into 2 groups. The patients who underwent TACE with I-DEM (25.7×10 Bq of 131iodine and 10 mg of doxorubicin) were compared to controls who received cTACE with PVA embolization microspheres. Therapeutic effects were evaluated by the tumor regression rates, levels of alpha-fetoprotein in serum, survival rates, and complications.

Results: The operative complications of the 2 groups were not significantly different (P=0.753). The radioactivity ratio of the tumor to the liver was approximately 4.1:1 for the I-DEM TACE group. In the I-DEM TACE group, 54.5% of patients achieved tumor regression of more than 50%, compared to 36.6% of patients in the cTACE group. AFP levels in serum declined in 100% of patients in the I-DEM TACE group and 50% of patients in the cTACE group. The median survival time of the patients was 12.0±3.3 months for the I-DEM TACE group and 10.0±3.3 months for the cTACE group. There were no significant differences in survival between the 2 groups (P=0.414).

Conclusions: I-DEM may become a potential radiochemoembolization agent to treat patients with unresectable hepatocellular carcinoma through TACE.
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http://dx.doi.org/10.21037/jgo-21-105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107628PMC
April 2021

High energy density and extremely stable supercapacitors based on carbon aerogels with 100% capacitance retention up to 65,000 cycles.

Proc Natl Acad Sci U S A 2021 May;118(21)

Institute of Materials, Ningbo University of Technology, 315016 Ningbo, People's Republic of China;

In terms of ideal future energy storage systems, besides the always-pursued energy/power characteristics, long-term stability is crucial for their practical application. Here, we report a facile and sustainable strategy for the scalable fabrication of carbon aerogels with three-dimensional interconnected nanofiber networks and rationally designed hierarchical porous structures, which are based on the carbonization of bacterial cellulose assisted by the soft template of Zn-1,3,5-benzenetricarboxylic acid. As binder-free electrodes, they deliver a fundamentally enhanced specific capacitance of 352 F ⋅ g at 1 A ⋅ g in a wide potential window (1.2 V, 6 M KOH) in comparison with those of bacterial cellulose-derived carbons (178 F ⋅ g) and most activated carbons (usually lower than 250 F ⋅ g). The as-assembled supercapacitors exhibit an ultrahigh capacitance of 297 F ⋅ g at 1 A ⋅ g, remarkable energy density (14.83 Wh ⋅ kg at 0.60 kW ⋅ kg), and extremely high stability, with 100% capacitance retention for up to 65,000 cycles at 6 A ⋅ g, representing their superior energy storage performance when compared with that of state-of-the-art supercapacitors of commercial activated carbons and biomass-derived analogs.
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http://dx.doi.org/10.1073/pnas.2105610118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166133PMC
May 2021

Improving the precision of optical metrology by detecting fewer photons with biased weak measurement.

Light Sci Appl 2021 May 17;10(1):103. Epub 2021 May 17.

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, 230026, Hefei, China.

In optical metrological protocols to measure physical quantities, it is, in principle, always beneficial to increase photon number n to improve measurement precision. However, practical constraints prevent the arbitrary increase of n due to the imperfections of a practical detector, especially when the detector response is dominated by the saturation effect. In this work, we show that a modified weak measurement protocol, namely, biased weak measurement significantly improves the precision of optical metrology in the presence of saturation effect. This method detects an ultra-small fraction of photons while maintains a considerable amount of metrological information. The biased pre-coupling leads to an additional reduction of photons in the post-selection and generates an extinction point in the spectrum distribution, which is extremely sensitive to the estimated parameter and difficult to be saturated. Therefore, the Fisher information can be persistently enhanced by increasing the photon number. In our magnetic-sensing experiment, biased weak measurement achieves precision approximately one order of magnitude better than those of previously used methods. The proposed method can be applied in various optical measurement schemes to remarkably mitigate the detector saturation effect with low-cost apparatuses.
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http://dx.doi.org/10.1038/s41377-021-00543-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128924PMC
May 2021

Incorporating Guanidinium as Perovskitizer-Cation of Two-Dimensional Metal Halide for Crystal-Array Photodetectors.

Chem Asian J 2021 Jul 1;16(14):1925-1929. Epub 2021 Jun 1.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, P. R. China.

Two-dimensional (2D) hybrid perovskites are recently emerging as a potential family of semiconductors for versatile optoelectronic applications. Currently, the "perovskitizer" moieties are rigidly limited to small-size cations, while few 2D metal-halides containing guanidinium cations inside perovskite cages have been studied for photodetection. Herein, we present a new 2D hybrid perovskite, (i-BA) (G)Pb I (where G is guanidinium and i-BA is isobutylammonium), which adopts a bilayered framework of {GPb I }. Single-crystal structure analyses disclose that G cations act as the perovskitizer, confined in the flexible perovskite cages formed by the distorted PbI octahedra. Such inorganic sheets are crucial to the superior semiconducting properties and optical bandgap, as verified by the density functional theory calculation. Furthermore, its planar crystal-array photodetector shows fascinating photoelectric performance, including a quite low dark current (∼4.6×10  A), a large current switching ratio (∼1.0×10 ), and a notable photo-responsivity of ∼0.72 A W , suggesting great potential of (i-BA) (G)Pb I for photodetection.
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http://dx.doi.org/10.1002/asia.202100425DOI Listing
July 2021

The role of PKA/PP2B-mediated Drp1 phosphorylation and the subsequent EGFR inhibition in Cr(VI)-induced premature senescence.

Ecotoxicol Environ Saf 2021 May 7;218:112300. Epub 2021 May 7.

Xiangya School of Public Health, Central South University, Changsha 410078, PR China. Electronic address:

In recent years, frequent hexavalent chromium [Cr(VI)] pollution incidents have severely damaged the ecology and endangered the public health. It is well known that cell senescence could promote the carcinogenesis, thus the related research on the occurrence of premature senescence is of great significance to the elucidation of the carcinogenic mechanism of Cr(VI). We previously confirmed that long-term low-dose Cr(VI) exposure induced premature senescence, but the key molecular events that determine the occurrence of premature senescence are still unclear. In the present study, we found that Cr(VI) induced phosphorylation of dynamin-relatedprotein 1 (Drp1)-S637 site in premature senescent cells, which was accompanied with the decrease of mitochondrial fission. We also demonstrated that the phosphorylation status of Drp1-S637 after Cr(VI) exposure was related to the antagonism of PKA/PP2B, and continuous dephosphorylation of Drp1-S637 attenuated premature senescence caused by Cr(VI). The epidermal growth factor receptor (EGFR) overexpression significantly alleviated the occurrence of premature senescence, and the expressions of EFGR and its downstream molecules were related to the phosphorylation status of Drp1-S637. In brief, we revealed the role of PKA/PP2B-mediated Drp1 phosphorylation and the subsequent EGFR inhibition in Cr(VI)-induced premature senescence. This study is the first time to link the phosphorylation of Drp1 with Cr(VI)-induced premature senescence, in order to find the key molecular events that determine the occurrence of premature senescence and demonstrate the molecular mechanism of abnormal elongated mitochondria formation in the senescence process. The significance of this study is to explore the carcinogenesis of Cr(VI) and provide new ideas and strategies for the targeted treatment of Cr(VI)-related cancers.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112300DOI Listing
May 2021

Epidemiological and clinical characteristics of imported cases of COVID-19: a multicenter study.

BMC Infect Dis 2021 May 3;21(1):406. Epub 2021 May 3.

Emergency Department, Qilu Hospital, Shandong University, Shandong Provincial Clinical Research Center for Emergency and Critical Care Medicine, Jinan, Shandong, 250012, China.

Background: The coronavirus disease 2019 (COVID-19) pandemic continues to expand. Herein, we report the epidemiological and clinical features of 478 patients with confirmed COVID-19 from a multicenter study conducted in four cities in China excluding Wuhan.

Methods: A total of 478 patients transferred by emergency medical services to designated hospitals in four major cities in China (Beijing, Chongqing, Jinan, and Nanning) were enrolled. We compared the characteristics of imported and indigenous cases and calculated the frequencies of fatal, severe, mild, and asymptomatic disease. The results were used to generate a pyramid of COVID-19 severity.

Results: The mean age of patients with COVID-19 was 46.9 years and 49.8% were male. The most common symptoms at onset were fever (69.7%), cough (47.5%), fatigue (24.5%), dyspnea (8.4%), and headache (7.9%). Most cases (313, 65.5%) were indigenous, while 165 (34.5%) were imported. Imported cases dominated during the early stages of the pandemic, but decreased from 1 February 2020 as indigenous cases rose sharply. Compared with indigenous cases, imported cases differed significantly in terms of sex (P = 0.002), severity of disease (P = 0.006), occurrence of fever (P < 0.001), family clustering (P < 0.001), history of contact (P < 0.001), and primary outcome (P < 0.001).

Conclusions: Within the population studied, imported cases had distinct characteristics from those of indigenous cases, with lower fatality rates and higher discharge rates. New infections shifted from imported cases to local infection gradually, and overall infections have declined to a low level. We suggest that preventing import of cases and controlling spread within local areas can help prevent SARS-CoV-2 infection spread.
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http://dx.doi.org/10.1186/s12879-021-06096-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090926PMC
May 2021

Two-Dimensional Guanidine-Based Hybrid Perovskites with Strong Dichroism for Multiwavelength Polarization-Sensitive Detection.

Chemistry 2021 Jun 27;27(36):9267-9271. Epub 2021 May 27.

College of Chemical Engineering, Fuzhou University, Fuzhou, Fujian, 350116, P. R. China.

Two-dimensional (2D) organic-inorganic hybrid perovskites, benefiting from their natural anisotropy of quantum-well motifs and optical properties, have shown remarkable polarization-dependent responses superior to the 3D counterparts. Here, for the first time, multiwavelength polarization-sensitive detectors were fabricated by using single crystals of a guanidine-based 2D hybrid perovskite, (BA) (GA)Pb I (where BA is n-butylammonium and GA is guanidium). Its unique 2D quantum-well structure results in strong crystallographic-dependence of optical absorption. Strikingly, our crystal-based photodetector exhibits a prominent photocurrent dichroic ratio (I /I ) of ∼2.2 at 520 nm, higher than the typical 2D inorganic materials (GeSe, ∼1.09, PdSe , ∼1.8). In addition, notable dichroic ratios of 1.29 and 1.23 at 405 nm and 637 nm are also created for the multiwavelength polarized-light detection. The prominent detecting performances, including low dark current (1.6×10  A), considerable on/off ratio (∼2×10 ), high photodetectivity (∼3.3×10 Jones) and responsivity (∼12.01 mA W ), make (BA) (GA)Pb I a promising candidate for polarized-light detection. This work sheds light on the rational engineering of new 2D hybrid perovskites for the high-performance optoelectronic device applications.
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http://dx.doi.org/10.1002/chem.202100691DOI Listing
June 2021

Transmission dynamics of the rubella virus circulating in China during 2010-2019: two lineage switches between genotypes 1E and 2B.

Clin Infect Dis 2021 Apr 27. Epub 2021 Apr 27.

Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.

Background: To provide a better understanding of the progress on rubella control and elimination in China, a genetic analysis was conducted to examine the transmission pattern of the endemic rubella virus in China during 2010-2019.

Methods: Total 4895 strains were obtained from 29 out of the 31 provinces in mainland of China during 2010-2019. The genotyping region of the strains were amplified, determined, and assembled. Genotyping analysis and lineage division were performed by comparisons with the World Health Organization reference strains and previously reported lineage reference strains, respectively. Further phylogenetic analyses were performed to compare the genetic relationship.

Results: During 2010-2019, the domestic lineage 1E-L1 and multiple imported lineages of rubella viruses including 2B-L1, 1E-L2, and 2B-L2c were identified. Further analysis of the circulation trend of the different lineages indicated that two switches occurred among the lineages. The first shift was from lineage 1E-L1 to 2B-L1, which occurred around 2015-2016, followed by the lowest rubella incidence in 2017. The second shift was from lineage 2B-L1 to 1E-L2 and 2B-L2c, which occurred around 2018-2019, coinciding with rubella resurgence and the subsequent nationwide epidemic during 2018-2019. Insufficient genomic information worldwide made it impossible to trace the origin of the imported viruses in this study.

Conclusions: China was moving toward rubella elimination, as evidenced by the fact that previous endemic lineages were not detected. However, rubella reemerged in 2018 and 2019 due to the newly imported rubella viruses. Therefore, to realize the rubella elimination goal, joint efforts are required for all countries worldwide.
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http://dx.doi.org/10.1093/cid/ciab339DOI Listing
April 2021

Efficacy and Safety of Thermal Ablation for Treatment of Solitary T1N0M0 Papillary Thyroid Carcinoma: A Multicenter Retrospective Study.

Radiology 2021 Jul 27;300(1):209-216. Epub 2021 Apr 27.

From the Department of Interventional Ultrasound Medicine, China-Japan Friendship Hospital, No. 2 Yinghuayuan East Street, Chaoyang District, Beijing 100029, China (X.J.C., Y.W., Z.L.Z., L.L.P., Y.L., M.A.Y.); Department of Medical Ultrasound, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China (S.R.W., L.Q.); Department of Ultrasound, the First Affiliated Hospital of Dalian Medical University, Dalian, China (Y.C., Y.L.Z.); Department of Thyroid, Beijing Beicheng Chinese Medicine Hospital, Beijing, China (J.L.); Department of Electrodiagnosis, the Affiliated Hospital to Changchun University of Traditional Chinese Medicine, Changchun, China (Z.B.C., X.W.); Department of Ultrasound, the First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology, Baotou, China (J.F.H., G.Z.Y.); Department of Breast and Thyroid Surgery, People's Hospital of Xinjiang Uygur Autonomous, Wulumuqi, China (H.L.W., A.S.); Department of Ultrasound, Wuhai People's Hospital, Wuhai, China (G.L.); Department of Interventional Ultrasound, Qinghai Provincial People's Hospital, Xining, China (J.Q.G.); Department of Ultrasound, Mudanjiang Tumor Hospital, Mudanjiang, China (Y.H., X.F.L.); Special Inspection Section, Wendeng District People's Hospital, Wendeng, China (Z.H.W.); First Department of Surgery, Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang, China (Y.Z.); Department of Ultrasound, Bayannur Hospital, Bayannur China (M.J.); and Department of Ultrasound, Laixi Municipal Hospital, Laixi, China (L.L.S.).

Background Microwave ablation (MWA) and radiofrequency ablation (RFA) have recently attracted interest as minimally invasive treatment modalities for papillary thyroid carcinoma (PTC). However, the ablation outcomes of T1N0M0 PTC are not well characterized. Purpose To evaluate the efficacy and safety of thermal ablation (MWA or RFA) of solitary T1N0M0 PTC in patients who were ineligible for (due to presence of comorbid cardiovascular disease, renal failure, other malignancy, etc) or who refused surgery. Materials and Methods This was a retrospective multicenter study of 847 patients (660 women) who underwent thermal ablation for PTC (673 T1a, 174 T1b) between March 2015 and March 2020; of these patients, 645 underwent MWA and 202 underwent RFA. The mean age of patients was 46 years ± 11 (standard deviation) (age range, 18-81 years); the mean follow-up time was 22 months ± 13 (range, 6-60 months). Changes in tumor size and volume and the rates of technical success, tumor disappearance, disease progression, and complications were assessed. Results The technical success rate was 100%. Relative to preablation measurements, the maximum diameter and volume of the ablation zone increased during the 1st month after ablation ( < .001), whereas there was no difference by the 3rd month; subsequently, the tumors showed reduction in size at 6, 9, and 12 months (all < .001). Complete disappearance of tumors occurred in 68% of patients (577 of 847; 69% [466 of 673] in the T1a group vs 64% [111 of 174] in the T1b group; < .001). The postablation disease progression rate was 1.1% (nine of 847 patients; 0.9% [six of 673 patients] in the T1a group vs 1.7% [three of 174 patients] in the T1b group; = .54). The overall complication rate was 3.4% (29 of 847 patients; 2.7% [18 of 673 patients] in the T1a group vs 6.3% [11 of 174 patients] in the T1b group; = .02). Conclusion This multicenter study provided evidence that thermal ablation is an effective and safe treatment option in selected -patients with solitary T1N0M0 papillary thyroid carcinoma. © RSNA, 2021 See also the editorial by Baek and Cho in this issue.
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http://dx.doi.org/10.1148/radiol.2021202735DOI Listing
July 2021

Case Report: A New Entity: Multiple Differentiated Variant of Papillary Thyroid Carcinoma With Advanced Clinical Behavior.

Front Endocrinol (Lausanne) 2021 7;12:654638. Epub 2021 Apr 7.

Thyroid and Parathyroid Surgery Center, West China Hospital, Sichuan University, Chengdu, China.

There are many histological morphological types of papillary thyroid carcinoma (PTC), but the most frequently seen types are conventional. A single PTC commonly has a conventional and/or a variant morphological pattern. PTC with multiple (more than two) well-differentiated morphological patterns are extremely rare. We herein report the rare case of a 48-year-old male with initial diaphragmatic, pancreatic, and liver tumors from PTC. Then, the PTC was discovered following resection of these tumors, an ultrasound-guided fine-needle aspiration (US-FNA) cytology of a huge mass in the thyroid's left lobe revealed a PTC. After postoperative recovery, physical and ultrasound examinations identified an irregular large nodule in the thyroid's isthmus and left lobe, several swollen lymph nodes in the left neck, a mass in the left gluteus maximus, and several masses in both the bilateral parotid and salivary regions. The US-FNA's pathological examination confirmed metastatic PTCs in the left gluteus maximus and bilaterally in the parotid and salivary glands. An 18-fluorodeoxyglucose positron-emission tomography and computed tomography scan revealed abnormal uptakes in numerous locations (e.g., thyroid's isthmus and left lobe, bilateral parotid gland, and subcutaneous tissues). The patient underwent palliative therapy-including total thyroidectomy, bilateral central neck dissection, left lateral neck dissection, and excision of the bilateral parotid and salivary glands. A whole-body scan post-therapeutic radioactive iodine ablation revealed exclusive thyroid bed uptake. The patient subsequently underwent thyroid stimulating hormone (TSH) repression therapy and chemotherapy with lenvatinib, and thereafter achieved stable clinical conditions. Further histopathological analysis of the PTC revealed multiple differentiated morphological patterns in the single tumor located in the isthmus and left lobe of the thyroid, and in some metastatic lesions. Different metastatic lesions also presented different morphological patterns of PTC. In conclusions, we identified a new entity of PTC as a multiple differentiated variant of PTC (MDV-PTC) with an aggressive clinical nature.
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http://dx.doi.org/10.3389/fendo.2021.654638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058471PMC
April 2021
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