Publications by authors named "Yu Luo"

529 Publications

Time-resolved photoelectron imaging of complex resonances in molecular nitrogen.

J Chem Phys 2021 Apr;154(14):144305

Elettra-Sincrotrone Trieste, SS 14, km 163.5, in Area Science Park, 34149 Basovizza, Trieste, Italy.

We have used the FERMI free-electron laser to perform time-resolved photoelectron imaging experiments on a complex group of resonances near 15.38 eV in the absorption spectrum of molecular nitrogen, N, under jet-cooled conditions. The new data complement and extend the earlier work of Fushitani et al. [Opt. Express 27, 19702-19711 (2019)], who recorded time-resolved photoelectron spectra for this same group of resonances. Time-dependent oscillations are observed in both the photoelectron yields and the photoelectron angular distributions, providing insight into the interactions among the resonant intermediate states. In addition, for most states, we observe an exponential decay of the photoelectron yield that depends on the ionic final state. This observation can be rationalized by the different lifetimes for the intermediate states contributing to a particular ionization channel. Although there are nine resonances within the group, we show that by detecting individual photoelectron final states and their angular dependence, we can identify and differentiate quantum pathways within this complex system.
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http://dx.doi.org/10.1063/5.0046577DOI Listing
April 2021

The Perceptions of Patients, Families, Doctors, and Nurses Regarding Malignant Bone Tumor Disclosure in China: A Qualitative Study.

J Transcult Nurs 2021 Apr 15:10436596211005532. Epub 2021 Apr 15.

School of Nursing, Army Medical University, Chongqing, Peoples Republic of China.

Introduction: Withholding the malignant bone tumors disclosure from patients is common in China. The purpose of the study was to explore the perceptions of patients, families, doctors, and nurses regarding the disclosure of malignant bone tumors in China.

Method: Semistructured interviews were conducted with 25 cases, consisting of 69 participants (14 patients, 25 family members, 17 doctors, and 13 nurses), and the transcripts were analysed using Colaizzi's (1978) descriptive phenomenological analysis process.

Results: Five themes were identified: (1) doctors preferred to inform the family members first, (2) family members' decisions depended on different situations, (3) the patients agreed that disclosure should be different for each person/personally, (4) care from nurses reduced the overreaction of patients, and (5) key points improve the informing quality.

Discussion: Family participatory cancer disclosure and multidisciplinary teams could improve the quality of cancer disclosure. Culturally congruent health care and culturally sensitive interventions in cancer disclosure are suggested.
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http://dx.doi.org/10.1177/10436596211005532DOI Listing
April 2021

Targeting Candida albicans in dual-species biofilms with antifungal treatment reduces Staphylococcus aureus and MRSA in vitro.

PLoS One 2021 8;16(4):e0249547. Epub 2021 Apr 8.

Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, United Kingdom.

Polymicrobial biofilms consisting of fungi and bacteria are frequently formed on endotracheal tubes and may contribute to development of ventilator associated pneumonia (VAP) in critically ill patients. This study aimed to determine the role of early Candida albicans biofilms in supporting dual-species (dual-kingdom) biofilm formation with respiratory pathogens in vitro, and investigated the effect of targeted antifungal treatment on bacterial cells within the biofilms. Dual-species biofilm formation between C. albicans and three respiratory pathogens commonly associated with VAP (Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus) was studied using quantitative PCR. It was shown that early C. albicans biofilms enhanced the numbers of E. coli and S. aureus (including methicillin resistant S. aureus; MRSA) but not P. aeruginosa within dual-species biofilms. Transwell assays demonstrated that contact with C. albicans was required for the increased bacterial cell numbers observed. Total Internal Reflection Fluorescence microscopy showed that both wild type and hyphal-deficient C. albicans provided a scaffold for initial bacterial adhesion in dual species biofilms. qPCR results suggested that further maturation of the dual-species biofilm significantly increased bacterial cell numbers, except in the case of E.coli with hyphal-deficient C. albicans (Ca_gcn5Δ/Δ). A targeted preventative approach with liposomal amphotericin (AmBisome®) resulted in significantly decreased numbers of S. aureus in dual-species biofilms, as determined by propidium monoazide-modified qPCR. Similar results were observed when dual-species biofilms consisting of clinical isolates of C. albicans and MRSA were treated with liposomal amphotericin. However, reductions in E. coli numbers were not observed following liposomal amphotericin treatment. We conclude that early C. albicans biofilms have a key supporting role in dual-species biofilms by enhancing bacterial cell numbers during biofilm maturation. In the setting of increasing antibiotic resistance, an important and unexpected consequence of antifungal treatment of dual-species biofilms, is the additional benefit of decreased growth of multi-drug resistant bacteria such as MRSA, which could represent a novel future preventive strategy.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249547PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031443PMC
April 2021

CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis.

Drug Dev Res 2021 Apr 5. Epub 2021 Apr 5.

Department of Ultrasonography, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, China.

Circular RNA FAT atypical cadherin 1 (circFAT1) has been reported to play vital roles in the progression of some cancers. However, the regulatory role and underlying mechanisms of circFAT1 in cervical cancer (CC) remain largely unknown. The expression of circFAT1, microRNA (miR)-409-3p and cyclin-dependent kinase 8 (CDK8) was detected using qRT-PCR and Western blot assays. Cell proliferation, apoptosis, migration and invasion in vitro were investigated using cell counting kit-8, colony formation, flow cytometry, and transwell assays, respectively. Western blot assay was used to determine the activation of ERK1/2 and p38 MAPK pathway. The interaction miR-409-3p and circFAT1 or CDK8 was confirmed by dual-luciferase reporter, pull-down or RIP assays. The effects of circFAT1 in vivo were determined using xenograft models. CircFAT1 was highly expressed in CC, and closely associated with poor prognosis. CircFAT1 knockdown resulted in the suppression of proliferation, migration and invasion, and promotion of apoptosis in CC cells via the inactivation of ERK1/2 and p38 MAPK pathway; also, circFAT1 silencing could inactivate this pathway and repressed CC tumor growth in vivo. Mechanistic analysis showed that circFAT1 directly sponged miR-409-3p and then relieved the repressive effect of miR-409-3p on its target CDK8. Furthermore, miR-409-3p inhibition reversed the effects of circFAT1 silencing on CC cells. Whereas, miR-409-3p overexpression impeded CC cell growth and motility, which was attenuated by CDK8. CircFAT1 promoted CC progression via activating ERK1/2 and p38 MAPK pathway through the miR-409-3p/CDK8 axis, suggesting a promising prognostic biomarker and therapeutic target for CC.
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http://dx.doi.org/10.1002/ddr.21816DOI Listing
April 2021

Complete mitochondrial genome of (Meigen) (Diptera, Tachinidae).

Mitochondrial DNA B Resour 2021 Mar 19;6(3):905-906. Epub 2021 Mar 19.

Key Laboratory of Medical Insects, Guizhou Medical University, Guiyang, China.

The complete mitochondrial genome of (Meigen), was analyzed by next-generation sequencing approach. Its mitogenome is 15,841 bp totally, which consists of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one non-coding control region. The nucleotide composition biases toward A and T, the overall A + T% was up to 80.9% of the entire mitogenome. Phylogenetic analysis supported the sister relationship between Goniini and Blondeliini. The result also suggested that the monophyly of the Exoristinae.
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http://dx.doi.org/10.1080/23802359.2021.1886020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995883PMC
March 2021

Amygdala-based Functional Network Reveals Dissociated Neural Correlates of Consensual and Idiosyncratic Emotional Movie Experiences.

Neurosci Bull 2021 Mar 26. Epub 2021 Mar 26.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, China; School of Psychology, Center for Studies of Psychological Application, and Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, Guangzhou, 510631, China.

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http://dx.doi.org/10.1007/s12264-021-00666-zDOI Listing
March 2021

miR-181a Mediates Inflammatory Gene Expression After Intracerebral Hemorrhage: An Integrated Analysis of miRNA-seq and mRNA-seq in a Swine ICH Model.

J Mol Neurosci 2021 Mar 23. Epub 2021 Mar 23.

Department of Emergency Medicine, Washington University in St. Louis, St. Louis, MO, USA.

Intracerebral hemorrhage (ICH) is a severe neurological disorder with no proven treatment. Inflammation after ICH contributes to clinical outcomes, but the relevant molecular mechanisms remain poorly understood. In studies of peripheral leukocyte counts and mRNA-sequencing (mRNA-seq), our group previously reported that monocytes and Interleukin-8 (IL-8) were important contributors to post-ICH inflammation. microRNA (miRNA) are powerful regulators of gene expression and promising therapeutic targets. We now report findings from an integrated analysis of miRNA-seq and mRNA-seq in peripheral blood mononuclear cells (PBMCs) from a swine ICH model. In 10 pigs, one PBMC sample was collected immediately prior to ICH induction and a second 6 h later; miRNA-seq and mRNA-seq were completed for each sample. An aggregate score calculation determined which miRNA regulated the differentially expressed mRNA. Networks of molecular interactions were generated for the combined miRNA/target mRNA. A total of 227 miRNA were identified, and 46 were differentially expressed after ICH (FDR < 0.05). The anti-inflammatory miR-181a was decreased post-ICH, and it was the most highly connected miRNA in the miRNA/mRNA bioinformatic network analysis. miR-181a has interconnected pathophysiology with IL-8 and monocytes; in prior studies, we found that IL-8 and monocytes contributed to post-ICH inflammation and ICH clinical outcome, respectively. miR-181a was a significant mediator of post-ICH inflammation and is promising for further study, including as a potential therapeutic target. This investigation also demonstrated feasible methodology for miRNA-seq/mRNA-seq analysis in swine that is innovative, and with unique challenges, compared with transcriptomics research in more established species.
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http://dx.doi.org/10.1007/s12031-021-01815-9DOI Listing
March 2021

Real-time use of a computer-aided system for polyp detection during colonoscopy, an ambispective study.

J Dig Dis 2021 Mar 20. Epub 2021 Mar 20.

Tianjin Clinical Medicine Research Centre for ITCWM Acute abdomen, Tianjin Hospital of ITCWM Nankai Hospital, Tianjin, China.

Objective: Lower miss rates correlate with lower incidence of post-colonoscopy colorectal cancers and related mortality. This study evaluated the effectiveness of real-time use of a computer-aided detection system on number of polyp per colonoscopy and polyp detection rate ambispectively.

Methods: Eighty-five videos were retrospectively marked with computer-aided detection. Unmarked videos were reviewed by 2 senior endoscopists. Polyps that were detected in marked and unmarked videos were re-counted in parallel. One endoscopist recruited 128 consecutive patients through prospective evaluation. The endoscopist used standard colonoscopy monitor or computer-aided detection monitor alternately every two weeks. During withdrawal, the endoscopist focused on standard monitor or computer-aided detection monitor for different groups. Numbers of polyp per colonoscopy and polyp detection rate between two groups were prospectively compared.

Results: Re-counting polyps per colonoscopy in unmarked and marked videos were 73 and 88, mean numbers polyp per colonoscopy were 0.86 and 1.04 (p = 0.001, tested by paired t-test) respectively. The increment proportion of polyp per colonoscopy was 20.5%, where 14.7% marked videos detected more polyps than unmarked videos. Of 128 patients enrolled in prospective study, 186 polyps were detected. The mean number of polyp per colonoscopy were higher in computer-aided detection than in standard colonoscopy (1.66 vs. 1.13, p = 0.039). The polyp detection rate of computer-aided detection colonoscopy was significantly higher than that of standard colonoscopy (78.1% vs. 56.3%, p = 0.008).

Conclusion: Real-time computer-aided detection system significantly increased polyp detection rate and number of polyp per colonoscopy in a relevant high polyp detection rate situation.
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http://dx.doi.org/10.1111/1751-2980.12985DOI Listing
March 2021

Assessment of the anti-inflammatory effects of three rhubarb anthraquinones in LPS-Stimulated RAW264.7 macrophages using a pharmacodynamic model and evaluation of the structure-activity relationships.

J Ethnopharmacol 2021 Jun 17;273:114027. Epub 2021 Mar 17.

Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China. Electronic address:

Ethnopharmacological Relevance: Rhubarb (Rhei Radix et Rhizoma) is a traditional Chinese medicine, has been used as a strong astringent in China to treat inflammation-related diseases, such as acute pancreatitis, acute cholecystitis, appendicitis and so on. Rhein, emodin and aloe-emodin are the important active anthraquinone in rhubarb, and are considered to be the main ingredients contributing to anti-inflammatory.

Aim Of The Study: Rhein, emodin and aloe-emodin, anthraquinones with the same parent structure that are found in rhubarb, have beneficial anti-inflammatory effects in vitro and in vivo. Anthraquinone derivatives also have important clinical roles. However, their pharmacodynamic differences and the structure-activity relationships associated with their anti-inflammatory properties have not been systematically explored. The present study was designed to quantify the effects of three rhubarb anthraquinones on inflammation and to explore the structure-activity relationships of these compounds.

Materials And Methods: In this study, we detected NF-κB phosphorylation, iNOS protein expression, and IL-6 and NO production in LPS-stimulated RAW264.7 cells and then calculated median effect equations and built a dynamic pharmacodynamic model to quantitatively evaluate the efficacy of these three anthraquinones. Additionally, to determine the structure-activity relationships, we investigated the physicochemical properties and molecular electrostatic potentials of the drug molecules.

Results: We found that rhein, emodin, and aloe-emodin exerted at least dual-target (NF-κB, iNOS) inhibition of LPS-induced inflammatory responses. Compared with rhein and emodin, aloe-emodin had a stronger anti-inflammatory effect, and its inhibition of iNOS protein expression was approximately twice that of NF-κB phosphorylation. In addition, aloe-emodin had the strongest hydrophobic effect among the three anthraquinones.

Conclusions: Overall, we concluded that the receptor binding the rhubarb anthraquinones had a hydrophobic pocket. Anthraquinone molecules with stronger hydrophobic effects had higher affinity for the receptor, resulting in greater anti-inflammatory activity. These results suggest that the addition of a hydrophobic group is a potential method for structural modification to design anti-inflammatory anthraquinone derivatives with enhanced potency.
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http://dx.doi.org/10.1016/j.jep.2021.114027DOI Listing
June 2021

NLRP3-mediated pyroptosis aggravates pressure overload-induced cardiac hypertrophy, fibrosis, and dysfunction in mice: cardioprotective role of irisin.

Cell Death Discov 2021 Mar 15;7(1):50. Epub 2021 Mar 15.

Department of Cardiology, Affiliated Hospital of North Sichuan Medical College, 637000, Nanchong, P.R. China.

The exact mechanism of myocardial hypertrophy has not been completely elucidated. NOD-like receptor protein 3 (NLRP3) and the pyroptotic cascade play a critical role in cardiac hypertrophy and inflammation. The myokine irisin can inhibit NLRP3 activation, although its exact mechanism of action is unknown. In this study, we induced cardiac hypertrophy in a mouse model via aortic constriction (TAC) to further explore the pathological role of NLRP3 inflammasome-mediated pyroptosis and the potential therapeutic effects of irisin. Cardiac hypertrophy significantly increased the percentage of apoptotic cells and upregulated IL-1β, cleaved caspase-1, and GSDMD-N that lie downstream of the NLRP3 inflammasome. Subsequently, irisin was co-administered to the TAC mice or angiotensin II (Ang-II)-treated cardiomyocytes to observe whether it could attenuate pyroptosis and cardiac hypertrophy. We established a direct association between pyroptosis and cardiac hypertrophy and found that pharmacological or genetic inhibition of NLRP3 attenuated cardiac hypertrophy. Furthermore, ectopic overexpression of NLRP3 abrogated the cardioprotective effects of irisin. To summarize, pyroptosis is a pathological factor in cardiac hypertrophy, and irisin is a promising therapeutic agent that inhibits NLRP3-mediated pyroptosis of cardiomyocytes.
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http://dx.doi.org/10.1038/s41420-021-00434-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961005PMC
March 2021

Media Exposure to COVID-19 Predicted Acute Stress: A Moderated Mediation Model of Intolerance of Uncertainty and Perceived Social Support.

Front Psychiatry 2020 10;11:613368. Epub 2021 Feb 10.

School of Psychology, Guizhou Normal University, Guiyang, China.

Previous studies have found that disaster-related media exposure could predict acute stress responses. However, few studies have investigated the relationship between media exposure to COVID-19 and acute stress, and less is known about the mechanisms that translate media exposure to COVID-19 into acute stress. The current study explored the impact of media exposure to COVID-19 on acute stress, and examined the mediating role of intolerance of uncertainty (IU) and the moderating role of perceived social support (PSS). A total of 1,483 Chinese participants ( = 27.93 years, = 8.45) completed anonymous online questionnaires regarding media exposure to COVID-19, IU, PSS, and acute stress during the COVID-19 outbreak in China. Media exposure to COVID-19 was positively related to acute stress, and IU partially mediated this relationship. The direct effect of media exposure to COVID-19 on acute stress, and the relationship between IU and acute stress, were both moderated by PSS. The impacts of both media exposure to COVID-19 and IU on acute stress were stronger for individuals with low PSS. This study collected data in a shorter timeframe, and no assessments occurred during the follow-up, which may prevent us from detecting the changes of the relationships between variables over time. Meanwhile, the self-report method limited the validity of the data due to subjective reporting bias. These findings contribute to a better understanding of how and when pandemic-related media exposure affects acute stress, and provide new perspectives for the prevention to reduce psychological problems following traumatic events.
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http://dx.doi.org/10.3389/fpsyt.2020.613368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902691PMC
February 2021

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus.

Chin J Nat Med 2021 Feb;19(2):81-89

Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China. Electronic address:

We isolated a novel lectin (AHL) from Artocarpus hypargyreusHance and showed its immunomodulatory activities. In this study, the amino acid sequence of AHL was determined by cDNA sequencing. AHL cDNA (875bp) contains a 456-bp open reading frame (ORF), which encodes a protein with 151 amino acids. AHL is a new member of jacalin-related lectin family (JRLs), which share high sequence similarities to KM+ and Morniga M, and contain the conserved carbohydrate binding domains. The antitumor activity of AHL was also explored using Jurkat T cell lines. AHL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bad and Bax up-regulation, and caspase-3 activation. We further showed that the activation of ERK and p38 signaling pathways is involved for the pro-apoptotic effect of AHL.
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http://dx.doi.org/10.1016/S1875-5364(21)60009-2DOI Listing
February 2021

PARP1-RNA interaction analysis: PARP1 regulates the expression of extracellular matrix-related genes in HK-2 renal proximal tubular epithelial cells.

FEBS Lett 2021 Feb 28. Epub 2021 Feb 28.

Department of Nephrology, Renmin Hospital of Wuhan University, China.

Recent studies suggest that Poly(ADP-ribose) polymerase 1 (PARP1) acts as an RNA-binding protein in a majority of renal diseases with tubular cell injury. However, detailed knowledge of RNA targets and the RNA-binding regions for PARP1 is unknown. Herein, mapping of iRIP-seq reads in HK-2 renal tubular epithelial cells showed a biased distribution at coding sequence (CDS) and intron regions that is specific to these cells. A total of 1708 differentially expressed genes were identified after PARP1 knockdown using RNA-seq. Furthermore, transcriptome analysis also showed that selective variable splicing was globally regulated by PARP1 in HK-2 cells. By comparison of PARP1 RNA-seq and iRIP-seq data, we found 68 overlapping genes that are enriched in 'extracellular matrix' pathway. Follow-up identification of their interactions may contribute vital insights into the regulatory role of PARP1 as an RNA-binding protein in HK-2 cells.
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http://dx.doi.org/10.1002/1873-3468.14065DOI Listing
February 2021

Dopaminergic and neurotrophic genetic polymorphisms modulate the implicit gender-science stereotype.

Psych J 2021 Feb 22. Epub 2021 Feb 22.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Beijing, China.

Genetic approaches to both the gender-science stereotype and implicit social cognition have received increasing attention in recent years. We explored whether single nucleotide polymorphisms (SNPs) in dopaminergic and neurotrophic systems (i.e., COMT, BDNF genotypes) explain variations in the implicit gender-science stereotype. We genotyped 413 adolescents and assessed their implicit gender-science stereotype with the Implicit Association Test. Replication on a subsample (N = 312) was conducted 2 years later. Results showed that SNP-level variations within the COMT and BDNF genes were consistently associated with the implicit gender-science stereotype in both investigations. These findings suggest that variants in the COMT and BDNF genes may contribute to the variation of implicit gender-science stereotype.
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http://dx.doi.org/10.1002/pchj.428DOI Listing
February 2021

A "sandwich" cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypes.

Biomater Sci 2021 Apr 12;9(7):2553-2561. Epub 2021 Feb 12.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Considering the key roles of macrophages in tissue repair and immune therapy, designing smart biomaterials able to harness macrophage phenotypes on demand during the healing process has become a promising strategy. Here, a novel "sandwich" cell culture platform with near-infrared (NIR) responsive dynamic stiffness was fabricated to polarize bone marrow-derived macrophages (BMDMs) in situ for revealing the relationship between the macrophage phenotype and substrate stiffness dynamically. Under NIR irradiation, calcium ions (Ca) diffused through the middle layer of the IR780-mixed phase change material (PCM) due to the photothermal effect of IR780, resulting in an increase of hydrogel stiffness in situ by the crosslinking of the upper layer of the hyaluronic acid-sodium alginate hydrogel (MA-HA&SA). The up-regulation of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α) was quantified by immunostaining and enzyme-linked immune sorbent assay (ELISA), respectively, indicating the transformation of macrophages from the anti-inflammatory to pro-inflammatory phenotype under dynamic stiffness. The nuclear Yes-associated-protein (YAP) ratio positively correlated with the shift of the macrophage phenotype. The modulation of macrophage phenotypes by stiffness-rise without the stimuli of cytokines offers an effective and noninvasive strategy to manipulate immune reactions to achieve optimized healing or therapeutic outcomes.
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http://dx.doi.org/10.1039/d0bm02194fDOI Listing
April 2021

Construction and characterization of Mesona chinensis polysaccharide-chitosan hydrogels, role of chitosan deacetylation degree.

Carbohydr Polym 2021 Apr 4;257:117608. Epub 2021 Jan 4.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

A novel kind of polyelectrolyte complex hydrogels was generated through polymerization of Mesona chinensis polysaccharide (MCP) and chitosan (CH) on the basis of physical crosslinking without the addition of ionic crosslinking agents or heat. Rheological measurement, Fourier transform infrared spectroscopy, X-ray diffraction, thermo-gravimetric analysis, and scanning electron microscopy were used to explore the properties of MCP-CH hydrogels. The effects of CH deacetylation degree (DD) on the gel properties and structural characteristics of MCP-CH hydrogels were also studied. Results showed that 0.5 % MCP and 1% CH could form stable and homogeneous hydrogels with favorable properties via electrostatic interaction. The viscoelasticity, water holding capacity, and thermostability of hydrogels were promoted with the increase in DD. The new crystallization peaks appeared with the formation of MCP-CH hydrogels. Moreover, the honeycomb-liked microstructure of MCP-CH hydrogels improved with the increase in DD. These findings may lay the foundation for the further application of MCP-CH hydrogels.
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http://dx.doi.org/10.1016/j.carbpol.2020.117608DOI Listing
April 2021

Quantitative susceptibility-weighted imaging may be an accurate method for determining stroke hypoperfusion and hypoxia of penumbra.

Eur Radiol 2021 Jan 29. Epub 2021 Jan 29.

Department of Radiology, Tianjin First Central Hospital, School of Medicine, Nankai University, Number 24 of Fukang Road, Nankai District, Tianjin, China.

Objectives: To quantitatively evaluate the volume of the ischemic penumbra using susceptibility-weighted imaging and mapping (SWIM) of asymmetrical prominent cortical veins (APCVs) in patients with acute ischemic stroke.

Methods: Eighty-five eligible patients with acute ischemic stroke on admission within 12 h from symptom onset were studied. The APCVs on SWIM were quantitatively (SWI-volume) and semi-quantitatively (SWI-Alberta Stroke Program Early CT Score, SWI-ASPECTS) evaluated to calculate mismatch. To assess the diagnostic efficacy of APCVs on SWIM, comparative analyses were performed between SWI-DWI mismatch and SWI-DWI mismatch, using PWI-DWI mismatch as a reference. Correlations were calculated between the mismatches, as well as between SWI-volume and time-to-maximum (Tmax) > 6 s volume. Additionally, each of these mismatches was correlated with the National Institute of Health Stroke Scale (NIHSS).

Results: The sensitivity, negative predictive value, and accuracy of SWI-DWI mismatch were demonstrably higher than SWI-DWI mismatch (100% vs. 53.7%, 100% vs. 9.5%, 97.7% vs. 54.5%, respectively). A significant positive correlation was found between SWI-DWI and PWI-DWI mismatch (r = 0.691, p < 0.01), as well as between SWI-volume and Tmax > 6 s volume (r = 0.786, p < 0.001). A significant negative correlation was found between SWI-DWI mismatch and NIHSS (r = - 0.360, p = 0.022), as well as between SWI-DWI mismatch and NIHSS (r = - 0.499, p = 0.001).

Conclusions: SWI-DWI mismatch had higher diagnostic efficacy than SWI-DWI mismatch in defining the ischemic penumbra and showed good consistency with PWI-DWI mismatch in acute ischemic stroke. Quantitation of APCVs using SWIM provided an accurate method for determining hypoperfusion and provided a reliable method to reflect the hypoxia of penumbra.

Key Points: • SWI-DWI mismatch has higher diagnostic efficacy than SWI-DWI mismatch in defining the ischemic penumbra. • SWI-DWI mismatch shows good consistency with PWI-DWI mismatch in managing penumbra in acute ischemic stroke. • Quantitation of APCV volume using SWIM provided an accurate method for determining the hypoperfusion area and provided a reliable method to reflect the hypoxia of penumbra.
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http://dx.doi.org/10.1007/s00330-020-07485-2DOI Listing
January 2021

The isolation of a DNA aptamer to develop a fluorescent aptasensor for the thiamethoxam pesticide.

Analyst 2021 Mar 27;146(6):1986-1995. Epub 2021 Jan 27.

CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.

Aptamers, which are called chemical antibodies for their high affinity and specificity to targets, have great potential as analytical tools to detect pesticides. In this work, a DNA aptamer for thiamethoxam was isolated by an improved SELEX (systematic evolution of ligands by exponential enrichment) strategy, in which the ssDNA library was fixed on streptavidin-agarose beads through a short biotin labeled complementary strand. After 13 rounds of selection, the random ssDNA pool was successfully enriched. Three sequences were chosen as aptamer candidates through sequencing and analysis and were transformed into fluorescent probes to evaluate their interactions with thiamethoxam. A fluorescent turn-on aptasensor for thiamethoxam based on the best aptamer (FAM-Thi13) and a short quenching strand were further designed and showed a quantitative linear range from 10 to 1000 nM with a detection limit of 1.23 nM for thiamethoxam. Molecular docking and molecular dynamics were used to investigate the binding site of the main probe of the aptasensor (FAM-Thi13) and thiamethoxam. Satisfactory results were also obtained in quantifying thiamethoxam in environmental water samples by the developed fluorescent aptasensor.
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http://dx.doi.org/10.1039/d0an01967dDOI Listing
March 2021

E7-Modified Substrates to Promote Adhesion and Maintain Stemness of Mesenchymal Stem Cells.

Macromol Biosci 2021 Apr 22;21(4):e2000384. Epub 2021 Jan 22.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Mesenchymal stem cells (MSCs) have drawn great attention in clinical applications due to the self-renewal ability, multi-differentiation potential, and low immunogenicity. However, there are challenges in the ex vivo expansion of MSCs, including low efficiency, stemness loss, and safety. Therefore, it is crucial to construct a substrate that can show an alterable affinity to MSCs, and induce efficient cell expansion with minimal stemness loss. In this study, EPLQLKM (E7)-modified substrates with tunable E7 densities are fabricated on PEGylated substrates. The PEG layer with an average thickness of 1.7 nm shows good antifouling ability. E7-modified substrates have an improving effect on adhesion and spreading of the rat bone marrow-derived mesenchymal stem cells (rBMSCs), along with the increase of E7 densities. rBMSCs on E7-modified substrates maintain the stem cell phenotypes, and shows robust proliferation and multilineage differentiation, especially on the substrates with high E7 densities. In summary, this study provides a novel strategy of E7 functionalization to promote adhesion and maintain stemness of MSCs, which holds great potentials in the functionalization of microcarriers for the expansion of MSCs.
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http://dx.doi.org/10.1002/mabi.202000384DOI Listing
April 2021

Factors and pathways regulating the release and transformation of arsenic mediated by reduction processes of dissimilated iron and sulfate.

Sci Total Environ 2021 May 9;768:144697. Epub 2021 Jan 9.

School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China; Institute of Environmental Geology, Inner Mongolia University, Hohhot 010021, China. Electronic address:

The driving process and explanatory factors regulating the transformation and migration of arsenic (As) mediated by dissimilatory iron reducing bacteria (DFeRB) and sulfate reducing bacteria (SRB) remain poorly understood. The novelty of this study is to explore the driving process and key environmental factors governing As mobilization mediated by DFeRB and SRB based on continuous As speciation and environmental parameter monitoring in a sediment-water system. The results illustrate the reduction process mediated by DFeRB and SRB significantly promotes the reduction of As(V) and the endogenous release of As. However, in the DFeRB and SRB mediated reductions, the main driving process and key explanatory factors that dominate the As mobility are significantly different. DFeRB has significant effects on the reductive dissolution and re-distribution of Fe(III) oxyhydroxides and As-containing Fe(III) minerals and on adsorption-desorption, which in turn influenced the transformation of iron species and the release and ecotoxicity of As. Meanwhile, the environmental factors that affect As mobility depend on Fe and Fe in DFeRB-induced reduction, presenting two main pathways: the process of As mobilization mediated by DFeRB, and the process influenced by the inorganic phosphorus involved in the competitive adsorption and anion exchange. Significantly different from DFeRB, the effects of SRB on As behavior mainly occur by influencing the adsorbed As, pyrite, and As sulfides in the sediments and through the formation of sulfides during the sulfate reduction. The main pathways of As mobilization reflect the direct effects of SRB, S, and Fe. In addition, the role of NH-N in the driving process of As mobility is more pronounced in SRB-induced reduction. NO-N is an essential factor affecting As mobility, but the effects of NO-N on As lead to non-significant pathways. This work provides insights into the environmental effects of DFeRB and SRB on the biogeochemical cycle of As.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144697DOI Listing
May 2021

Oxytocin intensifies the mortality salience effect: Novel evidence for the social salience model of oxytocin.

Horm Behav 2021 Mar 20;129:104920. Epub 2021 Jan 20.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Oxytocin plays an important role in human responses to threat processing. Few studies have directly examined the effects of oxytocin on our response to death-related stimuli. In the current study, 63 participants intranasally received either 32 IU of oxytocin or a placebo and thereafter completed a visual dot-probe task consisting of death-related and non-death related images. The results indicated that oxytocin enhanced participants' vigilance toward death-related images as well as increased their anxiety about and fear of death. Overall, oxytocin amplifies the defensive responses to a mortality threat, supporting the social salience model of oxytocin.
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http://dx.doi.org/10.1016/j.yhbeh.2020.104920DOI Listing
March 2021

[Short-term Outcome of T1bN0M0 Papillary Thyroid Cancer after Ultrasonography-guided Radiofrequency Ablation].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2020 Dec;42(6):771-775

School of Medicine,Nankai University,Tianjin 300071,China.

Objective To investigate the short-term outcome of T1bN0M0 papillary thyroid cancer after ultrasonography-guided radiofrequency ablation(RFA). Methods Eighty-nine patients with T1bN0M0 papillary thyroid cancer who were treated with ultrasonography-guided RFA in our center from April 2014 to January 2019 were retrospectively analyzed.Trans-isthmus approach and moving shot technique were used during the RFA procedure.Ultrasonography and contrast-enhanced ultrasonography were performed before ablation,and immediately,1,3,6 and 12 months after ablation and then every 6 months thereafter. Results RFA was performed in 89 cases of papillary thyroid cancer,and no major complications were observed during the RFA.The mean follow-up was(18.8±7.3)months.The ablation zones decreased gradually during follow-up,and 38 ablation zones(42.7%)completely disappeared.The volume reduction rate was(99.2±2.3)% 30 months after ablation.During follow-up,2 patients(2.2%)developed tumor recurrence and 1 patient(1.1%)developed cervical lymph node metastasis. Conclusion Ultrasonography-guided RFA may be a safe and effective method for patients with T1bN0M0 papillary thyroid cancer.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12731DOI Listing
December 2020

Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings.

Int J Biol Macromol 2021 Feb 6;171:448-456. Epub 2021 Jan 6.

College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agricultural and Forestry University, Fuzhou 350002, People's Republic of China. Electronic address:

To investigate the effects of Hericium erinaceus polysaccharide (HEP) on immunity in Muscovy duck reovirus (MDRV)-infected ducklings and explore its mechanism of action, an MDRV contact-infection model was established. Then, we investigated the influence of HEP on morphology of main immune organs in MDRV-infected ducklings by HE staining, while antioxidant capacity (T-AOC, MDA), serum protein levels (TP, ALB, GLO), complement levels (C, C) and antibody levels (IgA, IgM, IgG) were detected. Apoptotic indexes (apoptosisi rate and FAS-L) were also quantified by TUNEL method and immunohistochemical staining. Meanwhile, FADD and CytC (apoptosis-related genes), were tested by quantitative RT-PCR. Results showed that HEP could reduce the injuries of immune organs caused by MDRV. Additionally, HEP markedly diminished MDA (p < 0.01), while significantly increased T-AOC, TP, ALB, GLO, C, C, IgA, IgM and IgG (p < 0.01 or p < 0.05). Then, HEP shifted apoptosis time to an early MDRV-infected stage and reduced apoptosis at later MDRV-infected stage. This was associated with changes of FADD and CytC. Collectively, our data suggested that HEP could reduce the immunesuppression by many ways, such as decreasing organs' injuries, improving antioxidant capacity, serum proteins levels, antibody levels and complement levels, while diminish the apoptosis by lowering the FADD and CytC.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.12.222DOI Listing
February 2021

Premature deaths caused by smoking in Sichuan, Southwest China, 2015-2030.

Sci Rep 2021 Jan 8;11(1):171. Epub 2021 Jan 8.

Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.

Smoking has a substantial impact on deaths from non-communicable chronic diseases (NCDs). Quantitatively measuring the impact of tobacco control on population health is of great theoretical and practical importance, for governments to make health policy decisions. Focusing on premature deaths, we predicted the deaths by 2030 from major NCDs caused by smoking among people aged 30-69 years in Sichuan Province, Southwest China. We extracted data for 1990-2015 from the Global Burden of Disease Study 2015 and calculated the population attributable fraction, to estimate the proportion of deaths caused by smoking. Four different tobacco control standards were used to estimate 2030 projections for the prevalence of smoking and premature mortality. If smoking prevalence were reduced by 30% from 2015 levels, premature mortality could be expected to decline by 24.4% in 2030, achieving 81.3% of the World Health Organization target for reducing premature mortality by 30%. Compared with the continuation of historical trends, the strongest tobacco control policy scenario would reduce premature mortality by 6.6%, prevent 23,600 deaths, reduce mortality by 7.8%, and increase life expectancy at birth by 0.3 years. Smoking bans represent an important action toward achieving national health goals.
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http://dx.doi.org/10.1038/s41598-020-79606-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794491PMC
January 2021

Contrast-enhanced ultrasound: a valuable modality for extracapsular extension assessment in papillary thyroid cancer.

Eur Radiol 2021 Jan 7. Epub 2021 Jan 7.

Department of Ultrasound, Division of First Medical Center, Chinese People's Liberation Army General Hospital, 28 Fuxing Road, Beijing, 100853, China.

Objectives: To evaluate the diagnostic accuracy of preoperative contrast-enhanced ultrasound (CEUS) to detect extracapsular extension (ECE) and identify the relationship between ECE and nodule enhancement patterns in patients with papillary thyroid cancer (PTC).

Methods: Patients with suspected thyroid cancer underwent ultrasound (US) and CEUS examinations. The US and CEUS features of the PTC nodules and thyroid capsule were recorded and classified individually. The accuracy of US and CEUS in detecting ECE was compared individually, and its relationship with various tumour enhancement patterns was analysed. The presence or absence of ECE and cervical lymph node metastasis (LNM) was confirmed pathologically.

Results: The final dataset included 119 patients with 124 PTC nodules. Seventy-two (60.5%) of these patients with PTC had no ECE (including 38 patients with single capsule invasion), while the remaining 52 had ECE. A significant difference was found in nodules with non-capsule invasion, single capsule invasion, and ECE between the cervical LNM and non-LNM groups (p < 0.01). Receiver operating characteristic curve analysis demonstrated that area under the curve (AUC) of ECE for cervical LNM was higher than that of capsule invasion (71.9% vs. 49.6%). Moreover, the CEUS images acquired to detect ECE showed higher AUC values than those of US images (79.4% vs. 65.8%) (p = 0.02). Among the PTC nodules with differential enhancement, hyper-enhanced nodules had the highest incidence of capsule invasion (41.9%), while hypo-enhanced ones had a higher incidence of ECE (47.4%).

Conclusions: Compared with conventional US, CEUS is a more valuable and non-invasive imaging modality to detect ECE.

Key Points: • Single capsular invasion was a poor predictor of cervical lymph node metastasis, while extracapsular extension assessments were clinically significant for predicting cervical lymph node metastasis. • CEUS is better than conventional US in detecting extracapsular extension in papillary thyroid carcinoma (AUC: 79.4% vs. 65.8%) (p = 0.02). • Among the thyroid papillary carcinoma nodules with differential enhancement, hyper-enhanced nodules had the highest incidence of single capsule invasion (41.9%), while hypo-enhanced ones had a higher incidence of ECE (47.4%).
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http://dx.doi.org/10.1007/s00330-020-07516-yDOI Listing
January 2021

Multifunctional Role of Polyvinylpyrrolidone in Pharmaceutical Formulations.

AAPS PharmSciTech 2021 Jan 6;22(1):34. Epub 2021 Jan 6.

College of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China.

Polyvinylpyrrolidone (PVP), a non-ionic polymer, has been employed in multifarious fields such as paper, fibers and textiles, ceramics, and pharmaceutics due to its superior properties. Especially in pharmacy, the properties of inertness, non-toxicity, and biocompatibility make it a versatile excipient for both conventional formulations and novel controlled or targeted delivery systems, serving as a binder, coating agent, suspending agent, pore-former, solubilizer, stabilizer, etc. PVP with different molecular weights (MWs) and concentrations is used in a variety of formulations for different purposes. In this review, PVP-related researches mainly in recent 10 years were collected, and its main pharmaceutical applications were summarized as follows: (i) improving the bioavailability and stability of drugs, (ii) improving the physicomechanical properties of preparations, (iii) adjusting the release rate of drugs, and (iv) prolonging the in vivo circulation time of liposomes. Most of these applications could be explained by the viscosity, solubility, hydrophilicity, and hydrogen bond-forming ability of PVP, and the specific action mechanisms for each application were also tried to figure out. The effect of PVP on bioavailability improvement establishes it as a promising polymer in the emerging controlled or targeted formulations, attracting growing interest on it. Therefore, given its irreplaceability and tremendous opportunities for future developments, this review aims to provide an informative reference about current roles of PVP in pharmacy for interested readers.
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http://dx.doi.org/10.1208/s12249-020-01909-4DOI Listing
January 2021

The role of alkali in sweet potato starch-Mesona chinensis Benth polysaccharide gels: Gelation, rheological and structural properties.

Int J Biol Macromol 2021 Feb 28;170:366-374. Epub 2020 Dec 28.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang 330047, China. Electronic address:

According to the Chinese traditional black jelly production process, a critical step to ensure the quality of jelly is to add alkali to it. In this study, the effects of sodium carbonate (NaCO) and sodium bicarbonate (NaHCO) on the pasting, rheological, textural, and structural properties of sweet potato starch (SPS)-Mesona chinensis Benth polysaccharide (MCP) gels were evaluated. Adding NaCO at low concentration reduced the final viscosity (FV) of SPS-MCP gels, whereas adding it at a high concentration increased the FV. Adding NaHCO can increase the FV of SPS-MCP gels in a concentration-dependent manner. Rheological results indicated that adding NaHCO at a low concentration decreased the storage modulus of SPS-MCP gels, whereas adding it at a high concentration increased the storage modulus. The storage modulus of SPS-MCP gels increased with increasing concentration of NaHCO. The addition of NaCO and NaHCO improved the textural properties of SPS-MCP gels and decreased the water mobility. Infrared results indicated that adding alkali can enhance the hydrogen bonding between SPS and MCP. Scanning electron microscopy results suggested that alkali can reduce the size of gels, and make the structure more compact.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.12.166DOI Listing
February 2021

Dynamic oxygen-17 MRI with adaptive temporal resolution using golden-means-based 3D radial sampling.

Magn Reson Med 2021 Jun 25;85(6):3112-3124. Epub 2020 Dec 25.

Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA.

Purpose: The aim of this study was to develop a high-resolution 3D oxygen-17 ( O) MRI method to delineate the kinetics of O-enriched water (H O) across the entire mouse brain after a bolus injection via the tail vein.

Methods: The dynamic O signal was acquired with a golden-means-based 3D radial sampling scheme. To achieve adequate temporal resolution with preserved spatial resolution, a k-space-weighted view sharing strategy was used in image reconstruction with an adaptive window size tailored to the kinetics of the O signal. Simulation studies were performed to determine the adequate image reconstruction parameters. The established method was applied to delineating the kinetics of intravenously injected H O in vivo in the post-stroke mouse brain.

Results: The proposed dynamic O-MRI method achieved an isotropic resolution of 1.21 mm (0.77 mm nominal) in mouse brain at 9.4T, with the temporal resolution increased gradually from 3 s at the initial phase of rapid signal increase to 15 s at the steady-state. The high spatial resolution enabled the delineation of the heterogeneous H O uptake and washout kinetics in stroke-affected mouse brain.

Conclusion: The current study demonstrated a 3D O-MRI method for dynamic monitoring of O signal changes with high spatial and temporal resolution. The method can be utilized to quantify physiological parameters such as cerebral blood flow and blood-brain barrier permeability by tracking injected H O. It can also be used to measure oxygen consumption rate in O-oxygen inhalation studies.
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http://dx.doi.org/10.1002/mrm.28636DOI Listing
June 2021

FRET-MC: A fluorescence melting competition assay for studying G4 structures in vitro.

Biopolymers 2020 Dec 24:ebip23415. Epub 2020 Dec 24.

Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris, Palaiseau, France.

G-quadruplexes (G4) play crucial roles in biology, analytical chemistry and nanotechnology. The stability of G4 structures is impacted by the number of G-quartets, the length and positions of loops, flanking motifs, as well as additional structural elements such as bulges, capping base pairs, or triads. Algorithms such as G4Hunter or Quadparser may predict if a given sequence is G4-prone by calculating a quadruplex propensity score; however, experimental validation is still required. We previously demonstrated that this validation is not always straightforward, and that a combination of techniques is often required to unambiguously establish whether a sequence forms a G-quadruplex or not. In this article, we adapted the well-known FRET-melting assay to characterize G4 in batch, where the sequence to be tested is added, as an unlabeled competitor, to a system composed of a dual-labeled probe (F21T) and a specific quadruplex ligand. PhenDC3 was preferred over TMPyP4 because of its better selectivity for G-quadruplexes. In this so-called FRET-MC (melting competition) assay, G4-forming competitors lead to a marked decrease of the ligand-induced stabilization effect (∆T ), while non-specific competitors (e.g., single- or double-stranded sequences) have little effect. Sixty-five known sequences with different typical secondary structures were used to validate the assay, which was subsequently employed to assess eight novel sequences that were not previously characterized.
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http://dx.doi.org/10.1002/bip.23415DOI Listing
December 2020