Publications by authors named "Yu Long"

700 Publications

Corrigendum: Immune Phenotyping of Patients With Acute Vogt-Koyanagi-Harada Syndrome Before and After Glucocorticoids Therapy.

Front Immunol 2021 16;12:731824. Epub 2021 Jul 16.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.3389/fimmu.2021.659150.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.731824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323518PMC
July 2021

Marginal frequent itemset mining for fault prevention of railway overhead contact system.

ISA Trans 2021 Jul 13. Epub 2021 Jul 13.

Department of Electrical Engineering, Southwest Jiaotong University, Chengdu, China. Electronic address:

The overhead contact system (OCS), as the power source of electrified railway, has a complex composition and various types of faults, so it places high requirements on its fault prevention. In recent years, with the establishment of railway OCS fault database, association analysis has been used to implement fault prevention from system-wise perspective and provide guidance for operation and maintenance. However, due to the hierarchical structure of fault database, the existing frequent itemset mining has a lot of redundancy in the results, and cannot locate the most precise faults, which affects the decision-making and makes troubleshooting lack of pertinence. To address this issue, this paper proposed a new concept, called marginal frequent itemset, which is an itemset composed of as precise items as possible in hierarchical database that meets the threshold, and an alternative mining task: mining marginal frequent itemsets instead of all the frequent itemsets. Two methods, path transform and descending depth of itemset, are proposed for achieving mining a set of marginal frequent itemsets. Two novel measures, margin degree and marginal information quantity, are proposed to evaluate the content of the mining results. An efficient algorithm, named MFIM, is developed for mining cross-level marginal frequent itemsets from railway OCS fault database. Our performance study shows that MFIM has high performance and can obtain more key information and reduce the number of results. Furthermore, marginal frequent itemset mining can simplify the fault relation network constructed by association rules and optimize the decision-making process for fault prevention of railway OCS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isatra.2021.07.018DOI Listing
July 2021

Viscoelastic behaviour of rapid and slow self-healing hydrogels formed by densely branched arabinoxylans from Plantago ovata seed mucilage.

Carbohydr Polym 2021 Oct 9;269:118318. Epub 2021 Jun 9.

School of Chemical Engineering, University of Queensland, Brisbane, QLD 4076, Australia; School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, United Kingdom. Electronic address:

We report rheological characterisation of hydrogels formed by highly substituted brush-like arabinoxylans from Plantago ovata seed mucilage. Two arabinoxlyan fractions with similar molecular weight and linkage compositions are chosen to form gels with distinct rheological properties but a similar network structure. Small and large amplitude oscillatory shear rheology is used to characterise the sol-gel transition as a function of temperature and concentration. Differences in rheology and gelation of the two hydrogels are found to be associated with the different proportion of 'slow'- and 'fast'-dissociating junctions stabilised by hydrogen bonds, with the 'fast'-dissociating junctions playing an important role in rapid self-healing of the gel. Based on the temperature dependence of storage modulus and time-temperature superposition principle in combination with the Arrhenius equation, the activation energies of junction zone dissociation are estimated to be 402-480 kJ/mol and 97-144 kJ/mol for the 'slow' and 'fast' junction types, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118318DOI Listing
October 2021

Deciphering variation of 239 elite japonica rice genomes for whole genome sequences-enabled breeding.

Genomics 2021 Jul 6;113(5):3083-3091. Epub 2021 Jul 6.

Huazhi Biotech Co. Ltd., Changsha, Hunan 410125, China; China Southern Regional Collaborative Innovation Center for Cereal and Oil Crops, College of Agriculture, Hunan Agricultural University, Changsha, Hunan 410125, China. Electronic address:

Revealing genomic variation of representative and diverse germplasm is the cornerstone of deploying genomics information into genetic improvement programs of species of agricultural importance. Here we report the re-sequencing of 239 japonica rice elites representing the genetic diversity of japonica germplasm in China, Japan and Korea. A total of 4.8 million SNPs and PAV of 35,634 genes were identified. The elites from Japan and Korea are closely related and relatively less diverse than those from China. A japonica rice pan-genome was constructed, and 35 Mb non-redundant novel sequences were identified, from which 1131 novel genes were predicted. Strong selection signals of genomic regions were detected on most of the chromosomes. The heading date genes Hd1 and Hd3a have been artificially selected during the breeding process. The results from this study lay the foundation for future whole genome sequences-enabled breeding in rice and provide a paradigm for other species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2021.07.002DOI Listing
July 2021

Antibiotics modulate neoadjuvant therapy efficiency in patients with breast cancer: a pilot analysis.

Sci Rep 2021 Jul 7;11(1):14024. Epub 2021 Jul 7.

Department of Breast Center, The Fourth Hospital of Hebei Medical University, 169 Tianshan Street, Shijiazhuang, 050000, People's Republic of China.

Mounting evidence suggests that microbiota dysbiosis caused by antibiotic administration is a risk factor for cancer, but few research reports focus on the relationships between antibiotics and chemotherapy efficiency. We evaluated the influence of antibiotic administration on neoadjuvant therapy efficacy in patients with breast cancer (BC) in the present study. BC patients were stratified into two groups: antibiotic-treated and control based on antibiotic administration within 30 days after neoadjuvant therapy initiation. Disease-free survival (DFS) and overall survival (OS) were assessed using the Kaplan-Meier method, and the Cox proportional hazards model was used for multivariate analyses. The pathologic complete response rate of the control group was significantly higher than that of the antibiotic-treated group (29.09% vs. 10.20%, p = 0.017). Further univariate analysis with Kaplan-Meier calculations demonstrated that antibiotic administration was strongly linked with both reduced DFS (p = 0.04) at significant statistical levels and OS (p = 0.088) at borderline statistical levels. Antibiotic administration was identified as a significant independent prognostic factor for DFS [hazard ratio (HR) 3.026, 95%, confidence interval (CI) 1.314-6.969, p = 0.009] and OS (HR 2.836, 95% CI 1.016-7.858, p = 0.047) by Cox proportional hazards model analysis. Antibiotics that initiated reduced efficiency of chemotherapy were more noticeable in the HER2-positive subgroup for both DFS (HR 5.51, 95% CI 1.77-17.2, p = 0.003) and OS (HR 7.0395% CI 1.94-25.53, p = 0.003), as well as in the T3-4 subgroup for both DFS (HR 20.36, 95% CI 2.41-172.07, p = 0.006) and OS (HR 13.45, 95% CI 1.39-130.08, p = 0.025) by stratified analysis. Antibiotic administration might be associated with reduced efficacy of neoadjuvant therapy and poor prognosis in BC patients. As a preliminary study, our research made preparations for further understanding and large-scale analyses of the impact of antibiotics on the efficacy of neoadjuvant therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-93428-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263554PMC
July 2021

MYB77 regulates high-affinity potassium uptake by promoting expression of HAK5.

New Phytol 2021 Jun 30. Epub 2021 Jun 30.

State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475001, China.

In Arabidopsis, the high-affinity K transporter HAK5 is the major pathway for root K uptake when below 100 µM; HAK5 responds to Low-K (LK) stress by strongly and rapidly increasing its expression during K -deficiency. Therefore, positive regulators of HAK5 expression have the potential to improve K uptake under LK. Here, we show that mutants of the transcription factor MYB77 share a LK-induced leaf chlorosis phenotype, lower K content, and lower Rb uptake of the hak5 mutant, but not the shorter root growth, and that overexpression of MYB77 enhanced K uptake and improved tolerance to LK stress. Furthermore, we demonstrated that MYB77 positively regulates the expression of HAK5, by binding to the HAK5 promoter and enhances high-affinity K uptake of roots. As such, our results reveal a novel pathway for enhancing HAK5 expression under LK stress, and provides a candidate for increasing the tolerance of plants to LK.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.17589DOI Listing
June 2021

Multifunctional lanthanide metal-organic framework based ratiometric fluorescence visual detection platform for alkaline phosphatase activity.

Mikrochim Acta 2021 Jun 24;188(7):236. Epub 2021 Jun 24.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.

A turn-on/off ratiometric fluorescence detection platform based on multifunctional lanthanide metal-organic framework (Ln-MOF) and an enzymatic cascade reaction is proposed for alkaline phosphatase (ALP) activity assay. L-phosphotyrosine is hydrolyzed to levodopa (L-dopa) by two steps of enzymatic reaction. L-dopa further reacts with naphthoresorcinol to produce carboxyazamonardine with strong emission at 490 nm. In this process, multifunctional Ln-MOF ([email protected], BTC is the 1,3,5-benzenetricarboxylic acid) acts not only as a nanozyme to catalyze the fluorogenic reaction between L-dopa and naphthoresorcinol but also as a fluorescence internal standard. The emission of [email protected] at 620 nm is quenched by phosphate anions, and the dual-response ratiometric fluorescence (F/F) can be achieved. A good linear relationship was obtained between Δ(F/F) and ALP activity in the range 0.3-24 U L with the detection limit of 0.02 U L. In addition, a portable assay tube was designed for visual and point-of-care testing of ALP activity by color variation (ratiometric chromaticity). Both the ratiometric fluorescence detection and the visual detection methods were successfully applied to monitor ALP activity in human serum samples with recovery between 95.5%-109.0% and 94.0%-110.1%, and relative standard deviation less than 8.1% and 9.5%, respectively. As far as we know, this is the first report of ALP activity assay assisted by multifunctional Ln-MOF.Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-021-04880-4DOI Listing
June 2021

BCAT1 Activates PI3K/AKT/mTOR Pathway and Contributes to the Angiogenesis and Tumorigenicity of Gastric Cancer.

Front Cell Dev Biol 2021 7;9:659260. Epub 2021 Jun 7.

Department of Pancreatic and Gastric Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Focusing on antiangiogenesis may provide promising choices for treatment of gastric cancer (GC). This study aimed to investigate the mechanistic role of BCAT1 in the pathogenesis of GC, particularly in angiogenesis.

Methods: Bioinformatics and clinical samples analysis were used to investigate the expression and potential mechanism of BCAT1 in GC. BGC823 cells with BCAT1 overexpression or silencing were induced by lentiviral transduction. Cell phenotypes and angiogenesis were evaluated. The relevant proteins were quantized by Western blotting, immunohistochemistry, or immunofluorescence. Xenograft models were constructed to confirm the role of BCAT1 .

Results: BCAT1 was overexpressed in GC patients and associated with lower survival. BCAT1 expression was correlated with proliferation-, invasion-, or angiogenesis-related markers expression and pathways. Silencing BCAT1 expression suppressed cell viability, colony formation, cycle progression, invasion, and angiogenesis of BGC823 cells, as well as the tumor growth of xenograft models, whereas overexpressing BCAT1 had the opposite results both and . Bioinformatics analysis and Western blotting demonstrated that BCAT1 activated the PI3K/AKT/mTOR pathway. The addition of LY294002 reversed the tumor growth induced by BCAT1 overexpression, further verifying this mechanism.

Conclusion: BCAT1 might act as an oncogene by facilitating proliferation, invasion, and angiogenesis through activation of the PI3K/AKT/mTOR pathway. This finding could aid the optimization of antiangiogenesis strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.659260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215359PMC
June 2021

ULECGNet: An Ultra-Lightweight End-to-End ECG Classification Neural Network.

IEEE J Biomed Health Inform 2021 Jun 18;PP. Epub 2021 Jun 18.

ECG classification is a key technology in intelligent ECG monitoring. In the past, traditional machine learning methods such as SVM and KNN have been used for ECG classification, but with limited classification accuracy. Recently, the end-to-end neural network has been used for the ECG classification and shows high classification accuracy. However, the end-to-end neural network has large computational complexity including a large number of parameters and operations. Although dedicated hardware such as FPGA and ASIC can be developed to accelerate the neural network, they result in large power consumption, large design cost, or limited flexibility. In this work, we have proposed an ultra-lightweight end-to-end ECG classification neural network which has extremely low computational complexity (~8.2k parameters & ~227k MUL/ADD operations) and can be squeezed into a low-cost MCU (i.e. microcontroller) while achieving 99.1% overall classification accuracy. This outperforms the state-of-the-art ECG classification neural network. Implemented on a low-cost MCU (i.e. MSP432), the proposed design consumes only 0.4 mJ and 3.1 mJ per heartbeat classification for normal and abnormal heartbeats respectively for real-time ECG classification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/JBHI.2021.3090421DOI Listing
June 2021

Multifunctional nanoscale lanthanide metal-organic framework based ratiometric fluorescence paper microchip for visual dopamine assay.

Nanoscale 2021 Jul;13(25):11188-11196

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.

Dopamine (DA) plays a significant role in the human body and cerebral nervous system, and the accurate and rapid assay of DA is essential for the diagnosis of related diseases. Herein, we proposed a turn-on ratiometric fluorescent DA assay strategy by integrating a specific DA-resorcinol chemical reaction with a multifunctional lanthanide metal-organic framework (Ln-MOF). First, Eu-BTC (1,3,5-benzenetricarboxylic acid) was synthesized and further modified to obtain [email protected], which simultaneously plays multiple roles such as fluorescence internal standard, nanoreactor, cooperative catalysis effect and color shift enhancement. The [email protected] dispersion-based method exhibits ultra-sensitive (limit of detection, LOD is 0.01 μM) and wide-range linear response (0.04-30 μM) to DA in real serum. More importantly, it has excellent selectivity for DA, even in the presence of epinephrine and norepinephrine analogs. Thus, this method realizes the accurate and precise quantification of DA in serum (recoveries: 98.1%-110.1%, relative standard deviation RSD < 4.6%). Next, [email protected] was prepared into paper microchip, which has good storage stability (RSD < 3.5%, n = 3) in four weeks and achieves point-of-care visual DA assay coupled with smartphone-assisted portable detection device. The MOF paper microchip-based method shows low sample consumption (30 μL), high accuracy and precision for the quantification of DA in serum (recovery of 92.9%-106.2%, RSD < 5.3%), and gets the same assay results as the MOF dispersion-based method (relative error ≤ 6.83%). To our knowledge, this is the first time to propose the catalytic fluorescence turn-on detection strategy of DA based on a MOF paper microchip.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1nr02036fDOI Listing
July 2021

Recombinase polymerase amplification lateral flow dipstick (RPA-LF) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758).

Vet Parasitol 2021 Aug 1;296:109479. Epub 2021 Jun 1.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, 430070, Hubei, China; Key Laboratory of Animal Epidemical Disease and Infectious Zoonoses, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. Electronic address:

Babesiosis caused by Babesia orientalis, an intraerythrocytic apicomplexan protozoan, is one of the most important diseases for water buffalo in central and southern China, leading to huge economic losses, and its main diagnostic method is microscopic examination. In this study, a recombinase polymerase amplification - lateral flow dipstick (RPA-LF) assay, targeting the mitochondrial COXI gene of B. orientalis, was developed to detect B. orientalis in water buffalo. The RPA-LF assay was carried out as an isothermal reaction at 37 °C within 15 min. The specificity assay showed no cross-reactivity with other protozoa, and the sensitivity assay revealed the minimum detection limit was 0.25 parasite/μL, which was 40-fold more sensitive than that of conventional PCR (0.25 versus10 parasites/μL blood). Moreover, the RPA-LF method was successfully applied to test clinical samples, with no significant difference being observed between RPA-LF and conventional PCR results. Compared with conventional PCR, the novel RPA-LF method had the advantages of simple operation, short time, high sensitivity, and high specificity for B. orientalis detection, indicating the potential use of RPA-LF for rapid field detection of B. orientalis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetpar.2021.109479DOI Listing
August 2021

Radionuclide-based molecular imaging allows CAR-T cellular visualization and therapeutic monitoring.

Theranostics 2021 3;11(14):6800-6817. Epub 2021 May 3.

Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Chimeric antigen receptor T cell (CAR-T) therapy is a new and effective form of adoptive cell therapy that is rapidly entering the mainstream for the treatment of CD19-positive hematological cancers because of its impressive effect and durable responses. Huge challenges remain in achieving similar success in patients with solid tumors. The current methods of monitoring CAR-T, including morphological imaging (CT and MRI), blood tests, and biopsy, have limitations to assess whether CAR-T cells are homing to tumor sites and infiltrating into tumor bed, or to assess the survival, proliferation, and persistence of CAR-T cells in solid tumors associated with an immunosuppressive microenvironment. Radionuclide-based molecular imaging affords improved CAR-T cellular visualization and therapeutic monitoring through either a direct cellular radiolabeling approach or a reporter gene imaging strategy, and endogenous cell imaging is beneficial to reflect functional information and immune status of T cells. Focusing on the dynamic monitoring and precise assessment of CAR-T therapy, this review summarizes the current applications of radionuclide-based noninvasive imaging in CAR-T cells visualization and monitoring and presents current challenges and strategic choices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.56989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171102PMC
August 2021

Corrigendum to "Voltage-gated sodium channel Nav1.5 promotes tumor progression and enhances chemosensitivity to 5-fluorouracil in colorectal cancer" [Canc. Lett. 500 (2021) 119-131].

Cancer Lett 2021 Sep 3;516:57-60. Epub 2021 Jun 3.

Department of Colorectal Surgery, Sun Yat-sen University Cancer Center, Guangzhou, 510060, PR China; State Key Laboratory of Oncology in South China, Guangzhou, 510060, PR China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, PR China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2021.05.024DOI Listing
September 2021

Erythrocyte Adhesion of Merozoite Surface Antigen 2c1 Expressed During Extracellular Stages of .

Front Immunol 2021 17;12:623492. Epub 2021 May 17.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

, a major infectious agent of water buffalo hemolytic babesiosis, is transmitted by . However, no effective vaccine is available. Essential antigens that are involved in parasite invasion of host red blood cells (RBCs) are potential vaccine candidates. Therefore, the identification and the conduction of functional studies of essential antigens are highly desirable. Here, we evaluated the function of merozoite surface antigen 2c1 (BoMSA-2c1), which belongs to the variable merozoite surface antigen (VMSA) family in . We developed a polyclonal antiserum against the purified recombinant (r)BoMSA-2c1 protein. Immunofluorescence staining results showed that BoMSA-2c1 was expressed only on extracellular merozoites, whereas the antigen was undetectable in intracellular parasites. RBC binding assays suggested that BoMSA-2c1 specifically bound to buffalo erythrocytes. Cytoadherence assays using a eukaryotic expression system further verified the binding and inhibitory ability of BoMSA-2c1. We found that BoMSA-2c1 with a GPI domain was expressed on the surface of HEK293T cells that bound to water buffalo RBCs, and that the anti-rBoMSA2c1 antibody inhibited this binding. These results indicated that BoMSA-2c1 was involved in mediating initial binding to host erythrocytes of Identification of the occurrence of binding early in the invasion process may facilitate understanding of the growth characteristics, and may help in formulating strategies for the prevention and control of this parasite.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.623492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165267PMC
May 2021

Multi-Spectral Fusion and Denoising of Color and Near-Infrared Images Using Multi-Scale Wavelet Analysis.

Sensors (Basel) 2021 May 22;21(11). Epub 2021 May 22.

School of Electronic and Engineering, Xidian University, No. 2 South Taibai Road, Xi'an, Shaanxi 710071, China.

We formulate multi-spectral fusion and denoising for the luminance channel as a maximum a posteriori estimation problem in the wavelet domain. To deal with the discrepancy between RGB and near infrared (NIR) data in fusion, we build a discrepancy model and introduce the wavelet scale map. The scale map adjusts the wavelet coefficients of NIR data to have the same distribution as the RGB data. We use the priors of the wavelet scale map and its gradient as the contrast preservation term and gradient denoising term, respectively. Specifically, we utilize the local contrast and visibility measurements in the contrast preservation term to transfer the selected NIR data to the fusion result. We also use the gradient of NIR wavelet coefficients as the weight for the gradient denoising term in the wavelet scale map. Based on the wavelet scale map, we perform fusion of the RGB and NIR wavelet coefficients in the base and detail layers. To remove noise, we model the prior of the fused wavelet coefficients using NIR-guided Laplacian distributions. In the chrominance channels, we remove noise guided by the fused luminance channel. Based on the luminance variation after fusion, we further enhance the color of the fused image. Our experimental results demonstrated that the proposed method successfully performed the fusion of RGB and NIR images with noise reduction, detail preservation, and color enhancement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21113610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196879PMC
May 2021

Medical image segmentation using boundary-enhanced guided packet rotation dual attention decoder network.

Technol Health Care 2021 May 19. Epub 2021 May 19.

School of Educational Science, Xinjiang Normal University, Urumqi, Xinjiang, China.

Objective: The automatic segmentation of medical images is an important task in clinical applications. However, due to the complexity of the background of the organs, the unclear boundary, and the variable size of different organs, some of the features are lost during network learning, and the segmentation accuracy is low. To address these issues, This prompted us to study whether it is possible to better preserve the deep feature information of the image and solve the problem of low segmentation caused by unclear image boundaries.

Methods: In this study, we (1) build a reliable deep learning network framework, named BGRANet,to improve the segmentation performance for medical images; (2) propose a packet rotation convolutional fusion encoder network to extract features; (3) build a boundary enhanced guided packet rotation dual attention decoder network, which is used to enhance the boundary of the segmentation map and effectively fuse more prior information; and (4) propose a multi-resolution fusion module to generate high-resolution feature maps. We demonstrate the effffectiveness of the proposed method on two publicly available datasets.

Results: BGRANet has been trained and tested on the prepared dataset and the experimental results show that our proposed model has better segmentation performance. For 4 class classifification (CHAOS dataset), the average dice similarity coeffiffifficient reached 91.73%. For 2 class classifification (Herlev dataset), the prediction, sensitivity, specifificity, accuracy, and Dice reached 93.75%, 94.30%, 98.19%, 97.43%, and 98.08% respectively. The experimental results show that BGRANet can improve the segmentation effffect for medical images.

Conclusion: We propose a boundary-enhanced guided packet rotation dual attention decoder network. It achieved high segmentation accuracy with a reduced parameter number.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3233/THC-202789DOI Listing
May 2021

as biomarker in hepatitis B virus-associated hepatocellular carcinoma.

J Cancer 2021 19;12(12):3486-3500. Epub 2021 Apr 19.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.

Hepatitis B virus infection is associated with liver disease, including cancers. In this study, we assessed the power of sex-determining region Y (SRY)-related high-mobility group (HMG)-box 4() gene to predict the clinical course of hepatocellular carcinoma (HCC). To evaluate the differential expression of and its diagnostic and prognostic potential in HCC, we analyzed the GSE14520 dataset. Stratified analysis and joint-effect analysis were done using and clinical factor. We then designed a nomogram for predicting the clinical course of HCC. Differential expression and its correlation with tumor stage as well as its diagnostic and prognostic value were analyzed on the oncomine and GEPIA websites. Gene set enrichment analysis was explored as well as candidate gene ontology and metabolic pathways modulated by in HCC. Our analysis revealed that the level of was significantly upregulated in tumor issue ( <0.001). This observation was validated through oncomine dataset and MERAV analysis (all <0.05). Diagnostic receiver operating characteristic (ROC) analysis of suggested it has diagnostic potential in HCC (GSE14520 dataset: <0.001, area under curve (AUC) = 0.782; Oncomine: (Wurmbach dataset) = 0.002, AUC = 0.831 and (Mas dataset) <0.001, AUC = 0.947). In addition, exhibited high correlation with overall survival of HBV-associated HCC (adjusted = 0.004, hazard ratio (HR) (95% confidence interval (CI)) = 2.055 (1.261-3.349) and recurrence-free survival (adjusted = 0.008, HR (95% CI) = 1.721 (1.151-2.574). These observations which were verified by GEPIA analysis for overall survival ( = 0.007) and recurrence-free survival (= 0.096). Gene enrichment analysis revealed that affected processes included lymphocyte differentiation, pancreatic endocrine pathways, and insulin signaling pathway. prognostic value was evaluated using nomogram analysis for HCC 1, 3, and 5-year, survival. Differential expression presents an avenue of diagnosing and predicting clinical course of HCC. In HCC, may affect TP53 metabolic processes, lymphocyte differentiation and the insulin signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.46579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120190PMC
April 2021

Immune Phenotyping of Patients With Acute Vogt-Koyanagi-Harada Syndrome Before and After Glucocorticoids Therapy.

Front Immunol 2021 28;12:659150. Epub 2021 Apr 28.

Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Previous studies have established that disturbed lymphocytes are involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) syndrome. Accordingly, glucocorticoids (GCs), with their well-recognized immune-suppressive function, have been widely used for treatment of VKH patients with acute relapses. However, the systemic response of diverse immune cells to GC therapy in VKH is poorly characterized. To address this issue, we analyzed immune cell subpopulations and their phenotype, as well as cytokine profiles in peripheral blood from VKH patients (n=25) and health controls (HCs, n=21) by flow cytometry and luminex technique, respectively. For 16 patients underwent GC therapy (methylprednisolone, MP), the aforementioned measurements as well as the transcriptome data from patients before and after one-week's GC therapy were also compared to interrogate the systemic immune response to GC therapy. Lymphocyte composition in the blood was different in VKH patients and HCs. VKH patients had significantly higher numbers of T cells with more activated, polarized and differentiated phenotype, more unswitched memory B cells and monocytes, as compared to HCs. MP treatment resulted in decreased frequencies of T cells and NK cells, inhibited NK cell activation and T cell differentiation, and more profoundly, a marked shift in the distribution of monocyte subsets. Collectively, our findings suggest that advanced activation and differentiation, as well as dysregulated numbers of peripheral lymphocytes are the major immunological features of VKH, and GC therapy with MP not only inhibits T cell activation directly, but also affects monocyte subsets, which might combinatorically result in the inhibition of the pathogenic immune response.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.659150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113950PMC
April 2021

Upregulation of Nogo-B by hypoxia inducible factor-1 and activator protein-1 in hepatocellular carcinoma.

Cancer Sci 2021 Jul 21;112(7):2728-2738. Epub 2021 May 21.

Department of Clinical Laboratory, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, China.

Nogo-B is an important regulator of tumor angiogenesis. Expression of Nogo-B is remarkably upregulated in multiple tumor types, especially hepatocellular carcinoma (HCC). Here, we show the transcriptional regulation mechanisms of Nogo-B in liver cancer. In response to hypoxia, expression of Nogo-B significantly increased in HCC tissues and cells. The distal hypoxia-responsive element in the promoter was essential for transcriptional activation of Nogo-B under hypoxic conditions, which is the specific site for hypoxia inducible factor-1α (HIF-1α) binding. In addition, Nogo-B expression was associated with c-Fos expression in HCC tissues. Nogo-B expression was induced by c-Fos, yet inhibited by a dominant negative mutant A-Fos. Deletion and mutation analysis of the predicted activator protein-1 binding sites revealed that functional element mediated the induction of Nogo-B promoter activity, which was confirmed by ChIP. These results indicate that HIF-1α and c-Fos induce the expression of Nogo-B depending on tumor microenvironments, such as hypoxia and low levels of nutrients, and play a role in upregulation of Nogo-B in tumor angiogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cas.14941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253276PMC
July 2021

A new species of from Myanmar and description of the actual male of Thorell, 1897 (Araneae, Mesothelae, Liphistiidae).

Zookeys 2021 14;1031:41-58. Epub 2021 Apr 14.

Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.

Five Schiödte, 1849 species of the primitively segmented spider family Liphistiidae are currently known from Myanmar. Here, we described a new species, (♂♀), which was collected from Pyin Oo Lwin, Mandalay Region, Myanmar, diagnosed based on its genital morphology. The specimens (2♂♂, 5♀♀) collected by Walter C. Sedgwick from Pyin Oo Lwin in 1982 were misidentified as Thorell, 1897 and are treated here as the newly described species. Accordingly, we described the males of for the first time, redescribed its female, using newly collected specimens from Yadò, Than Taung and Kalekho Atet townships, Kayin State. We also provided information on the variation in genital morphology of both species, and their relationships within the -group of species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.1031.59102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060243PMC
April 2021

Effects of orthopedic insoles on patients with knee osteoarthritis: A meta-analysis and systematic review.

J Rehabil Med 2021 May 18;53(5):jrm00191. Epub 2021 May 18.

Department of Rehabilitation, Shanghai Xuhui Central Hospital, 200031 Shanghai, China.

Objective: Recent clinical evidence supports that orthopaedic insoles, especially lateral-wedge insoles, can significantly benefit patients with knee osteoarthritis. The aim of this study is to explore the effects of orthopaedic insoles in patients with knee osteoarthritis.

Methods: Randomized controlled trials evaluating the effects of orthopaedic insoles on patients with knee osteoarthritis, published up to 16 February 2021, were reviewed and outcomes quantitatively summarized.

Results: A total of 15 studies from 13 randomized controlled trials that involved 1,086 participants were included in this study. All the included studies exhibited a moderate bias risk and were of acceptable quality. The pooled mean difference of pain determined by the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) was -1.21 (p < 0.001, 95% confidence interval (95% CI) -2.61-0.18) with a high heterogeneity (I2 = 75%). In the sensitivity analysis, the overall incidence was -0.20 (p= 0.62, 95% CI= -0.87-0.46) with an accepted heterogeneity (I2 = 0%). No difference was observed between the Asian and Caucasian groups (p= 0.28). No significant difference was found in the pain score, Lequesne index or functional improvements.

Conclusion: Meta-analysis revealed that orthopaedic insoles do not provide relief of pain or improve functionality in patients with knee osteoarthritis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2340/16501977-2836DOI Listing
May 2021

Laboratory predictors of severe Coronavirus Disease 2019 and lung function in followed-up.

Clin Respir J 2021 Apr 25. Epub 2021 Apr 25.

Department of Geriatric Respiratory and Critical Care, the First Affiliated Hospital of Anhui Medical University, Hefei, PR China.

Background: A pandemic caused by SARS-CoV-2 has infected more than 79 million people and killed exceeding 1.7 million people around the world by the end of 2020.

Method: We obtained the clinical data of all diagnosed patients and lung function test of followed-up patients in Fuyang, Anhui province to investigate laboratory predictors of severe Coronavirus Disease 2019 (COVID-19) and the impairment of lung function.

Results: Of the 155 patients, 87 (56.13%) were males. The mean age was 41.95 (SD 15.34) years. Only 30 (19.35%) patients had the critical condition. Fever (84.52%) was the most common symptoms, and short of breath was more common in severe patients (p < 0.01). Lymphopenia was observed in most patients (74, 47.7%). It showed the elevation of CRP in 100 (64.5%) patients, the elevation of SAA or IL-6 in 104 (67.1%) patients. The calculated cut-off value of CRP was 19.35 mg/ml, the AUC was 0.777, sensitivity was 73.3%, specificity was 69.6%; SAA was 73.55 mg/L, 0.679, 83.3%, 56.8%, respectively; IL-6 was 18.85 pg/ml, 0.797, 83.3%, 64.8%; D-Dimer was 0.325 mg/L, 0.673, 66.7% and 68.8%. The combination of CRP, SAA, IL-6, and D-Dimer was 0.823 in AUC, 73.3% in sensitivity, and 78.4% in specificity. 12 (42.86%) followed-up patients had completely normal lung function indicators.

Conclusion: Elevated CRP, SAA, IL-6 and D-Dimer can be predictors to severe COVID-19. The combination of these four indicators can improve the effectivity and specificity of assessing severe COVID-19. Most of the followed-up patients showed no abnormalities in lung function test. Abnormal lung function is mainly reflected in the diffusion function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/crj.13381DOI Listing
April 2021

Borneol in cardio-cerebrovascular diseases: Pharmacological actions, mechanisms, and therapeutics.

Pharmacol Res 2021 Jul 21;169:105627. Epub 2021 Apr 21.

TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, PR China. Electronic address:

With the coming acceleration of global population aging, the incidence rate of cardio-cerebrovascular diseases (CVDs) is increasing. It has become the leading cause of human mortality. As a natural drug, borneol (BO) not only has anti-inflammatory, anti-oxidant, anti-apoptotic, anti-coagulant activities and improves energy metabolism but can also promote drugs to enter the target organs or tissues through various physiological barriers, such as the blood-brain barrier (BBB), mucous membrane, skin. Thus, it has a significant therapeutic effect on various CVDs, which has been confirmed in a large number of studies. However, the pharmacological actions and mechanisms of BO on CVDs have not been fully investigated. Hence, this review summarizes the pharmacological actions and possible mechanisms of BO, which provides novel ideas for the treatment of CVDs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phrs.2021.105627DOI Listing
July 2021

Genome-wide association mapping for resistance to bacterial blight and bacterial leaf streak in rice.

Planta 2021 Apr 8;253(5):94. Epub 2021 Apr 8.

Southern Regional Collaborative Innovation Center for Grain and Oil Crops and College of Agronomy, Hunan Agricultural University, Changsha, China.

Main Conclusion: Using genome-wide SNP association mapping, a total of 77 and 7 loci were identified for rice bacterial blight and bacterial leaf streak resistance, respectively, which may facilitate rice resistance improvement. Bacterial blight (BB) and bacterial leaf streak (BLS) caused by Gram-negative bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), respectively, are two economically important diseases negatively affecting rice production. To mine new sources of resistance, a set of rice germplasm collection consisting of 895 re-sequenced accessions from the 3000 Rice Genomes Project (3 K RGP) were screened for BB and BLS resistance under field conditions. Higher levels of BB resistance were observed in aus/boro subgroup, whereas the japonica, temperate japonica and tropical japonica subgroups possessed comparatively high levels of resistance to BLS. A genome-wide association study (GWAS) mined 77 genomic loci significantly associated with BB and 7 with BLS resistance. The phenotypic variance (R) explained by these loci ranged from 0.4 to 30.2%. Among the loci, 7 for BB resistance were co-localized with known BB resistance genes and one for BLS resistance overlapped with a previously reported BLS resistance QTL. A search for the candidates in other novel loci revealed several defense-related genes that may be involved in resistance to BB and BLS. High levels of phenotypic resistance to BB or BLS could be attributed to the accumulation of the resistance (R) alleles at the associated loci, indicating their potential value in rice resistance breeding via gene pyramiding. The GWAS analysis validated the known genes underlying BB and BLS resistance and identified novel loci that could enrich the current resistance gene pool. The resources with strong resistance and significant SNPs identified in this study are potentially useful in breeding for BB and BLS resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00425-021-03612-5DOI Listing
April 2021

miR-98-5p inhibits gastric cancer cell stemness and chemoresistance by targeting branched-chain aminotransferases 1.

Life Sci 2021 Jul 31;276:119405. Epub 2021 Mar 31.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, PR China. Electronic address:

Aims: Gastric cancer stem cells (GCSCs) have been used as a therapeutic target. This study aims to estimate the role of miR-98-5p (termed miR-98) in the development of GCSCs.

Main Methods: The expression of miR-98 in CD44 GCSCs was verified by RT-PCR. The miR-98 was overexpressed in CD44 GCSCs by Lentivirus. The ability of self-renewal, invasion, chemoresistance and tumorigenicity was detected in vitro or in vivo after overexpression of miR-98. The target genes of miR-98 were predicted and verified by luciferase reporter assays. The effects miR-98/BCAT1 signaling on the chemoresistance and tumorigenicity of CD44 GCSCs were investigated in a xenograft model by rescue experiments.

Key Findings: We have shown that miR-98 was decreased in CD44 GCSCs. The overexpression of miR-98 could inhibit the expression of stem-related genes and the ability of self-renewal, invasion, and tumorigenicity of GCSCs. Also, we found that miR-98 overexpression enhances the sensitivity to cisplatin treatment in vitro. Using a xenograft model, we showed that miR-98 overexpression reversed paclitaxel resistance to CD44 GCSCs. Finally, we found that branched-chain aminotransferases 1 (BCAT1) is a target gene of miR-98. Overexpressed BCAT1 reversed xenograft tumor formation ability and attenuated the paclitaxel chemosensitivity induced by miR-98 downregulation. Furthermore, BCAT1 restoration affected the expression of invasion and drug resistance-related genes.

Significance: This study revealed miR-98 inhibits gastric cancer cell stemness and chemoresistance by targeting BCAT1, suggesting that this miR-98/BCAT1 axis represents a potential therapeutic target in gastric cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119405DOI Listing
July 2021

GABA signalling modulates stomatal opening to enhance plant water use efficiency and drought resilience.

Nat Commun 2021 03 29;12(1):1952. Epub 2021 Mar 29.

Plant Transport and Signalling Lab, ARC Centre of Excellence in Plant Energy Biology, Waite Research Institute, Glen Osmond, SA, Australia.

The non-protein amino acid γ-aminobutyric acid (GABA) has been proposed to be an ancient messenger for cellular communication conserved across biological kingdoms. GABA has well-defined signalling roles in animals; however, whilst GABA accumulates in plants under stress it has not been determined if, how, where and when GABA acts as an endogenous plant signalling molecule. Here, we establish endogenous GABA as a bona fide plant signal, acting via a mechanism not found in animals. Using Arabidopsis thaliana, we show guard cell GABA production is necessary and sufficient to reduce stomatal opening and transpirational water loss, which improves water use efficiency and drought tolerance, via negative regulation of a stomatal guard cell tonoplast-localised anion transporter. We find GABA modulation of stomata occurs in multiple plants, including dicot and monocot crops. This study highlights a role for GABA metabolism in fine tuning physiology and opens alternative avenues for improving plant stress resilience.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21694-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007581PMC
March 2021

Simultaneous differential network analysis and classification for matrix-variate data with application to brain connectivity.

Biostatistics 2021 Mar 26. Epub 2021 Mar 26.

Department of Statistics, School of Management, Fudan University, Shanghai, 200433, China.

Growing evidence has shown that the brain connectivity network experiences alterations for complex diseases such as Alzheimer's disease (AD). Network comparison, also known as differential network analysis, is thus particularly powerful to reveal the disease pathologies and identify clinical biomarkers for medical diagnoses (classification). Data from neurophysiological measurements are multidimensional and in matrix-form. Naive vectorization method is not sufficient as it ignores the structural information within the matrix. In the article, we adopt the Kronecker product covariance matrices framework to capture both spatial and temporal correlations of the matrix-variate data while the temporal covariance matrix is treated as a nuisance parameter. By recognizing that the strengths of network connections may vary across subjects, we develop an ensemble-learning procedure, which identifies the differential interaction patterns of brain regions between the case group and the control group and conducts medical diagnosis (classification) of the disease simultaneously. Simulation studies are conducted to assess the performance of the proposed method. We apply the proposed procedure to the functional connectivity analysis of an functional magnetic resonance imaging study on AD. The hub nodes and differential interaction patterns identified are consistent with existing experimental studies, and satisfactory out-of-sample classification performance is achieved for medical diagnosis of AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/biostatistics/kxab007DOI Listing
March 2021

Cloning and expression of gene in two varieties.

J Genet 2021 ;100

College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, People's Republic of China.

is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The fulllength cDNAs of in conventional Lonicera macranthoides () and the mutant '' cultivar () were obtained using rapid amplification of cDNA ends and the expression vectors for were constructed to investigate the roles of in the cultivar. (GenBank: MK419948) and (GenBank: MK419948) were isolated from the conventional variety and '' cultivar respectively. is 1274 bp in length, whereas is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_like domain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerase chain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the genes were also expressed in stems and leaves. is underexpressed at flowering period 5 that the key time node for corolla expansion and nonexpansion, while is overexpressed during this flowering period. was speculated to be a functional gene causing different phenotypes in the two varieties. The successfully constructed plant expression vector provides an experimental reference for further research on the function of this gene and the basis for the excellent phenotype of ''.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2021

Urban Air Pollution Mapping Using Fleet Vehicles as Mobile Monitors and Machine Learning.

Environ Sci Technol 2021 04 24;55(8):5579-5588. Epub 2021 Mar 24.

School for Environment and Sustainability, University of Michigan, Ann Arbor, Michigan 48109-1382, United States.

Spatially explicit urban air quality information is important for developing effective air quality control measures. Traditionally, urban air quality is measured by networks of stationary monitors that are not universally available and sparsely sited. Mobile air quality monitoring using equipped vehicles is a promising alternative but has focused on vehicle-level experiments and lacks fleet-level demonstration. Here, we equipped 260 electric vehicles in a ride-hailing fleet in Beijing, China with low-cost sensors to collect real-time, spatial-resolved data on fine particulate matter (PM) concentrations. Using this data, we developed a decision tree model to infer the distribution of PM concentrations in Beijing at 1 km by 1 km and 1 h resolution. Our results are able to show both short- and long-term variations of urban PM concentrations and identify local air pollution hotspots. Compared with a benchmark model that only uses data from stationary monitoring sits, our model has shown significant improvement with the coefficient of determination increased from 0.56 to 0.80 and root mean square error decreased from 12.6 to 8.1 μg/m. To the best of our knowledge, this study collects the largest mobile sensor data for urban air quality monitoring, which are augmented by state-of-the-art machine learning techniques to derive high-quality urban air pollution mapping. Our results demonstrate the potential and necessity of using fleet vehicles as routine mobile sensors combined with advanced data science methods to provide high-resolution urban air quality monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c08034DOI Listing
April 2021

Biomimetic oxygen delivery nanoparticles for enhancing photodynamic therapy in triple-negative breast cancer.

J Nanobiotechnology 2021 Mar 20;19(1):81. Epub 2021 Mar 20.

Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Triple-negative breast cancer (TNBC) is a kind of aggressive breast cancer with a high rate of metastasis, poor overall survival time, and a low response to targeted therapies. To improve the therapeutic efficacy and overcome the drug resistance of TNBC treatments, here we developed the cancer cell membrane-coated oxygen delivery nanoprobe, CCm-HSA-ICG-PFTBA, which can improve the hypoxia at tumor sites and enhance the therapeutic efficacy of the photodynamic therapy (PDT), resulting in relieving the tumor growth in TNBC xenografts.

Results: The size of the CCm-HSA-ICG-PFTBA was 131.3 ± 1.08 nm. The in vitro O and ROS concentrations of the CCm-HSA-ICG-PFTBA group were both significantly higher than those of the other groups (P < 0.001). In vivo fluorescence imaging revealed that the best time window was at 24 h post-injection of the CCm-HSA-ICG-PFTBA. Both in vivo F-FMISO PET imaging and ex vivo immunofluorescence staining results exhibited that the tumor hypoxia was significantly improved at 24 h post-injection of the CCm-HSA-ICG-PFTBA. For in vivo PDT treatment, the tumor volume and weight of the CCm-HSA-ICG-PFTBA with NIR group were both the smallest among all the groups and significantly decreased compared to the untreated group (P < 0.01). No obvious biotoxicity was observed by the injection of CCm-HSA-ICG-PFTBA till 14 days.

Conclusions: By using the high oxygen solubility of perfluorocarbon (PFC) and the homologous targeting ability of cancer cell membranes, CCm-HSA-ICG-PFTBA can target tumor tissues, mitigate the hypoxia of the tumor microenvironment, and enhance the PDT efficacy in TNBC xenografts. Furthermore, the HSA, ICG, and PFC are all FDA-approved materials, which render the nanoparticles highly biocompatible and enhance the potential for clinical translation in the treatment of TNBC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12951-021-00827-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981819PMC
March 2021
-->