Publications by authors named "Yu Liu"

4,911 Publications

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Acute kidney disease in hospitalized acute kidney injury patients.

PeerJ 2021 24;9:e11400. Epub 2021 May 24.

Department of Nephrology, The Second Xiangya Hospital of Central South University; Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan, China.

Background: Acute kidney injury (AKI) and chronic kidney disease (CKD) have become worldwide public health problems, but little information is known about the epidemiology of acute kidney disease (AKD)-a state in between AKI and CKD. We aimed to explore the incidence and outcomes of hospitalized patients with AKD after AKI, and investigate the prognostic value of AKD in predicting 30-day and one-year adverse outcomes.

Methods: A total of 2,556 hospitalized AKI patients were identified from three tertiary hospitals in China in 2015 and followed up for one year.AKD and AKD stage were defined according to the consensus report of the Acute Disease Quality Initiative 16 workgroup. Multivariable regression analyses adjusted for confounding variables were used to examine the association of AKD with adverse outcomes.

Results: AKD occurred in 45.4% (1161/2556) of all AKI patients, 14.5% (141/971) of AKI stage 1 patients, 44.6% (308/691) of AKI stage 2 patients and 79.6% (712/894) of AKI stage 3 patients. AKD stage 1 conferred a greater risk of Major Adverse Kidney Events within 30 days (MAKE30) (odds ratio [OR], 2.36; 95% confidence interval 95% CI [1.66-3.36]) than AKD stage 0 but the association only maintained in AKI stage 3 when patients were stratified by AKI stage. However, compared with AKD stage 0, AKD stage 2-3 was associated with higher risks of both MAKE30 and one-year chronic dialysis and mortality independent of the effects of AKI stage with OR being 31.35 (95% CI [23.42-41.98]) and 2.68 (95% CI [2.07-3.48]) respectively. The association between AKD stage and adverse outcomes in 30 days and one year was not significantly changed in critically ill and non-critically ill AKI patients. The results indicated that AKD is common among hospitalized AKI patients. AKD stage 2-3 provides additional information in predicting 30-day and one-year adverse outcomes over AKI stage. Enhanced follow-up of renal function of these patients may be warranted.
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http://dx.doi.org/10.7717/peerj.11400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158174PMC
May 2021

The Short-Term Patency Rate of a Saphenous Vein Bridge Using the No-Touch Technique in off-Pump Coronary Artery Bypass Grafting in Vein Harvesting.

Int J Gen Med 2021 3;14:2281-2288. Epub 2021 Jun 3.

Department of Cardiac Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, People's Republic of China.

Objective: This study aimed to examine the short-term effect of the no-touch technique on the patency rate of a great saphenous vein (GSV) bridge used during off-pump coronary artery bypass grafting (OPCABG).

Methods: Between June 2018 and September 2020, 140 patients undergoing OPCABG, with grafts obtained from the GSV using the "no-touch" technique or the left internal mammary artery (LIMA), were enrolled in this study. The early clinical results and short-term patency rate of the OPCABG were evaluated at a three-month follow-up by comparing the patency rate of the LIMA bridge and the GSV bridge obtained by the no-touch technique. This study also analyzed the impacts of the postoperative complications of the lower limbs and the distribution area of diseased vessels on the patency rate of a GSV bridge obtained by the no-touch technique at an early stage.

Results: No perioperative death or adverse cardiovascular or cerebrovascular events occurred in the 140 patients undergoing OPCABG. The difference in the early patency rate between the GSV bridge obtained by the no-touch technique and the LIMA bridge was not statistically significant (95.9% vs 97.1%, p = 0.501). There was no significant difference in the patency rate between an end-to-side anastomosed venous bridge and a LIMA bridge (95.0% [248/261] vs 97.1% [136/140], p = 0.314). The overall patency rate of a no-touch vein bridge in the right coronary artery region was lower than it was in the left coronary artery region (93.8% [165/176] vs 97.9% [183/187], p = 0.049).

Conclusion: The no-touch technique may improve the early patency rate of a GSV bridge, and its effect is similar to that of a LIMA bridge.
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http://dx.doi.org/10.2147/IJGM.S311249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184136PMC
June 2021

[Effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):529-534

Department of Critical Care Medicine, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology (Xiaogan Central Hospital), Xiaogan 432000, Hubei, China. Corresponding author: Yao Peng, Email:

Objective: To evaluate the effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats.

Methods: Forty male Sprague-Dawley (SD) rats were selected and intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) was used to establish sepsis model. 0, 30, 45, 60, 75 dB noise stimulation or 0, 50, 100, 200, 400 Lux light stimulation were given to rats (all n = 4). The serum levels of cortisol and melatonin, and the cerebral content of Evans blue (EB) were measured 96 hours after the stimulation to determine the optimal intensity of intervention. The other 40 SD rats were randomly divided into control group (Con group), LPS group, noise intervention group (LPS+60 dB group), 200 Lux light intervention group (LPS+200 Lux group) and mechanical stimulation group (LPS+MS group), with 8 rats in each group. The open fields test and fear conditioning test were used to evaluate the exploratory behavior and cognitive function 96 hours after corresponding stimulation. The enzyme linked immunosorbent assay (ELISA) was used to detect cerebral level of interleukin-6 (IL-6) and serum levels of cortisol and melatonin. The blood-brain barrier integrity was assessed by EB staining. The protein levels of ZO-1, Claudin-5 and caspase-3 in the hippocampus were detected by Western blotting to assess the blood-brain barrier integrity and neuronal apoptosis.

Results: Compared with 0 dB group or 0 Lux group, the serum melatonin concentration in 60 dB group and 200 Lux group were significantly reduced, while the serum cortisol concentration and cerebral EB content were significantly increased. Therefore, 60 dB noise and 200 Lux light were selected in the subsequent experiments. Compared with Con group, the horizontal score and vertical score in the open field test in LPS group were significantly decreased. There were no significant differences in the proportion of freezing time, the cerebral contents of EB and IL-6, the serum levels of melatonin and cortisol, and the hippocampal expressions of ZO-1, Claudin-5 and caspase-3. Compared with LPS group, the horizontal score, vertical score and the percentage of freezing time in LPS+60 dB group, LPS+200 Lux group and LPS+MS group were significantly reduced [horizontal score: 73.8±9.7, 80.3±9.4, 64.5±8.3 vs. 103.6±15.5; vertical score: 9.4±1.7, 11.2±1.9, 6.8±0.9 vs. 15.9±2.8; the percentage of freezing time: (45.3±4.7)%, (53.3±5.8)%, (42.1±5.1)% vs. (66.1±6.3)%], the serum level of melatonin was significantly decreased (ng/L: 53.62±6.20, 44.25±6.41, 45.33±5.84 vs. 74.39±7.54), the serum level of cortisol was significantly increased (nmol/L: 818.34±95.53, 710.04±65.41, 989.73±91.63 vs. 398.82±72.59), the levels of EB, IL-6 in the brain tissue were significantly increased [EB (μg/g): 2.80±0.35, 2.38±0.31, 3.24±0.42 vs. 1.59±0.26; IL-6 (ng/g): 31.56±4.11, 26.69±3.75, 37.47±4.56 vs. 16.28±2.69], the expressions of ZO-1 and Claudin-5 were significantly decreased (ZO-1/β-actin: 0.37±0.04, 0.32±0.05, 0.24±0.04 vs. 0.80±0.09; Claudin-5/β-actin: 0.62±0.08, 0.47±0.06, 0.35±0.05 vs. 0.97±0.20), and the expression of cleaved caspase-3 was significantly increased (caspase-3/β-actin: 0.56±0.06, 0.39±0.04, 0.72±0.12 vs. 0.20±0.03), with statistically significant differences (all P < 0.05).

Conclusions: 60 dB noise, 200 Lux light or mechanical stimulation for 96 hours could inhibit the secretion of serum melatonin, promote the secretion of cortisol, and activate neuroinflammation in septic rats, and lead to neuronal apoptosis in hippocampus and hyper-permeability of blood-brain barrier, which in turn could cause sleep disturbance and cognitive impairment.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200630-00514DOI Listing
May 2021

16S rRNA Gene Amplicon Sequencing of Gut Microbiota from Naked Carp (Gymnocypris przewalskii) in Qinghai Lake, China.

Microbiol Resour Announc 2021 Jun 10;10(23):e0037421. Epub 2021 Jun 10.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

Naked carp (Gymnocypris przewalskii) is a second-grade animal under state protection of China. We report 16S rRNA gene amplicon analysis of the gut microbiota of Gymnocypris przewalskii. The three most abundant phyla are , , and , and the six most abundant genera are , , , , , and .
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http://dx.doi.org/10.1128/MRA.00374-21DOI Listing
June 2021

YB1 regulates miR-205/200b-ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma.

Cancer Commun (Lond) 2021 Jun 10. Epub 2021 Jun 10.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, P. R. China.

Background: Y-box binding protein 1 (YB1 or YBX1) plays a critical role in tumorigenesis and cancer progression. However, whether YB1 affects malignant transformation by modulating non-coding RNAs remains largely unknown. This study aimed to investigate the relationship between YB1 and microRNAs and reveal the underlying mechanism by which YB1 impacts on tumor malignancy via miRNAs-mediated regulatory network.

Methods: The biological functions of YB1 in hepatocellular carcinoma (HCC) cells were investigated by cell proliferation, wound healing, and transwell invasion assays. The miRNAs dysregulated by YB1 were screened by microarray analysis in HCC cell lines. The regulation of YB1 on miR-205 and miR-200b was determined by quantitative real-time PCR, dual-luciferase reporter assay, RNA immunoprecipitation, and pull-down assay. The relationships of YB1, DGCR8, Dicer, TUT4, and TUT1 were identified by pull-down and coimmunoprecipitation experiments. The cellular co-localization of YB1, DGCR8, and Dicer were detected by immunofluorescent staining. The in vivo effect of YB1 on tumor metastasis was determined by injecting MHCC97H cells transduced with YB1 shRNA or shControl via the tail vein in nude BALB/c mice. The expression levels of epithelial to mesenchymal transition markers were detected by immunoblotting and immunohistochemistry assays.

Results: YB1 promoted HCC cell migration and tumor metastasis by regulating miR-205/200b-ZEB1 axis partially in a Snail-independent manner. YB1 suppressed miR-205 and miR-200b maturation by interacting with the microprocessors DGCR8 and Dicer as well as TUT4 and TUT1 via the conserved cold shock domain. Subsequently, the downregulation of miR-205 and miR-200b enhanced ZEB1 expression, thus leading to increased cell migration and invasion. Furthermore, statistical analyses on gene expression data from HCC and normal liver tissues showed that YB1 expression was positively associated with ZEB1 expression and remarkably correlated with clinical prognosis.

Conclusion: This study reveals a previously undescribed mechanism by which YB1 promotes cancer progression by regulating the miR-205/200b-ZEB1 axis in HCC cells. Furthermore, these results highlight that YB1 may play biological functions via miRNAs-mediated gene regulation, and it can serve as a potential therapeutic target in human cancers.
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http://dx.doi.org/10.1002/cac2.12164DOI Listing
June 2021

Class IIa HDAC Downregulation Contributes to Surgery-Induced Cognitive Impairment Through HMGB1-Mediated Inflammatory Response in the Hippocampi of Aged Mice.

J Inflamm Res 2021 31;14:2301-2315. Epub 2021 May 31.

Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People's Republic of China.

Objective: Perioperative neurocognitive disorders (PND) are a common complication in the elderly. Histone deacetylases (HDACs) are a class of enzymes that control the acetylation status of intracellular proteins. Thus, we explored whether HDACs trigger the release of high mobility group box 1 (HMGB1) through altering the acetylation status in the hippocampi of aged mice.

Materials And Methods: The effect of the Class IIa HDAC in PND was explored using an in vivo form of splenectomy. Sixteen-month-old healthy male C57BL/6J mice were randomly divided into five groups: control, anesthesia plus sham surgery, anesthesia plus splenectomy, LMK235 treatment, and PBS treatment. The hippocampi were harvested on either first, third, or seventh postoperative day. Cognitive function was assessed via a Morris water maze (MWM) test. Quantitative RT-PCR, Western blots and ELISAs were carried out to assess the targeted gene expression at transcriptional and translational levels.

Results: Splenectomy led to a significant deficiency in spatial memory acquisition, marked decreases in mRNA and protein levels of HDAC4 and HDAC5 in the hippocampus, and increases in the levels of total HMGB1 and acetylated HMGB1. In a similar fashion to splenectomy, treatment with the HDAC4/5 inhibitor LMK235 produced impaired spatial memory and an increase in the expression of HMGB1 and its acetylated counterpart in the hippocampus.

Conclusion: These results suggest that surgery leads to PND through class IIa HDAC downregulation-triggered HMGB1 release in hippocampus of aged mice. HDACs may be a potential therapeutic target for postoperative cognitive dysfunction.
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http://dx.doi.org/10.2147/JIR.S304060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180279PMC
May 2021

Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.

Acta Pharmacol Sin 2021 Jun 8. Epub 2021 Jun 8.

Department of Nephrology, Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.

Tubulointerstitial inflammation plays an important role in the progression of diabetic nephropathy (DN), and tubular epithelial cells (TECs) are crucial promoters of the inflammatory cascade. Exchange protein activated by cAMP (Epac) has been shown to suppress the angiotensin II (Ang-II)-induced release of inflammatory cytokines in tubular cells. However, the role of Epac in TEC-mediated tubulointerstitial inflammation in DN remains unknown. We found that administering the Epac agonist 8-pCPT-2'-O-Me-cAMP (8-O-cAMP) to db/db mice inhibited tubulointerstitial inflammation characterized by macrophage infiltration and increased inflammatory cytokine release and consequently alleviated tubulointerstitial fibrosis in the kidney. Furthermore, 8-O-cAMP administration restored CCAAT/enhancer binding protein β (C/EBP-β) expression and further upregulated the expression of Suppressor of cytokine signaling 3 (SOCS3), while inhibiting p-STAT3, MCP-1, IL-6, and TNF-α expression in the kidney cortex in db/db mice. And in vitro study showed that macrophage migration and MCP-1 expression induced by high glucose (HG, 30 mM) were notably reduced by 8-O-cAMP in human renal proximal tubule epithelial (HK-2) cells. In addition, 8-O-cAMP treatment restored C/EBP-β expression in HK-2 cells and promoted C/EBP-β translocation to the nucleus, where it transcriptionally upregulated SOCS3 expression, subsequently inhibiting STAT3 phosphorylation. Under HG conditions, siRNA-mediated knockdown of C/EBP-β or SOCS3 in HK-2 cells partially blocked the inhibitory effect of Epac activation on the release of MCP-1. In contrast, SOCS3 overexpression inhibited HG-induced activation of STAT3 and MCP-1 expression in HK-2 cells. These findings indicate that Epac activation via 8-O-cAMP ameliorates tubulointerstitial inflammation in DN through the C/EBP-β/SOCS3/STAT3 pathway.
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http://dx.doi.org/10.1038/s41401-021-00689-2DOI Listing
June 2021

High-performance supercapacitor based on highly active P-doped one-dimension/two-dimension hierarchical NiCoO/NiMoO for efficient energy storage.

J Colloid Interface Sci 2021 May 18;601:793-802. Epub 2021 May 18.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:

Multi-dimensional metal oxides have become a promising alternative electrode material for supercapacitors due to their inherent large surface area. Herein, P-doped NiCoO/NiMoO multi-dimensional nanostructures are synthesized on carbon clothes (CC) with a continuous multistep strategy. Especially, P has the best synergistic effect with transition metals, such as optimal deprotonation energy and OH adsorption energy, which can further enhance electrochemical reaction activity. For the above reasons, the P-NiCoO/[email protected] electrode exhibits an ultra-high specific capacitance of 2334.0 F g at 1 A g. After 1500 cycles at a current density of 10 A g, its specific capacity still maintains 93.7%. Besides, a P-NiCoO/[email protected]//activated carbon device (hybrid supercapacitor or device) was also prepared with a maximum energy density of 45.1 Wh kg at a power density of 800 W kg. In particular, the capacity retention rate is still 89.97% after 8000 cycles due to its excellent structural stability. Our work demonstrates the vast potential of multi-dimensional metal oxides in energy storage.
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http://dx.doi.org/10.1016/j.jcis.2021.05.095DOI Listing
May 2021

Bovine viral diarrhea virus NS4B protein interacts with 2CARD of MDA5 domain and negatively regulates the RLR-mediated IFN-β production.

Virus Res 2021 Jun 4:198471. Epub 2021 Jun 4.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China; Heilongjiang Provincial Technology Innovation Center for Bovine Disease Control and Prevention, Daqing 163319, China; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing 163319, China.; Heilongjiang Province Cultivating Collaborative Innovation Center for The Beidahuang Modern Agricultural Industry Technology, Daqing 163319, China. Electronic address:

Bovine viral diarrhea virus (BVDV) is an important member of the family Flaviviridae and often causes immunosuppression. Previous studies have suggested that BVDV envelope protein E and the nonstructural autoprotease N can inhibit host innate immune responses. Herein, we found that BVDV NS4B, as a nonstructural protein necessary for replication, is involved in antagonizing the main RNA virus sensing pathway. Overexpression of BVDV NS4B protein significantly inhibited Sendai virus (SeV)-induced interferon-β promoter activity, IFN-β mRNA and IFN regulatory factor 3 (IRF3) phosphorylation levels. We also discovered that BVDV NS4B protein significantly inhibited RIG-I like receptor (RLRs)-mediated interferon-β (IFN-β) promoter activity and endogenous MDA5 mRNA levels. In addition, the BVDV NS4B protein directly interacts with N-terminal CARDs of MDA5, and co-localized with MDA5 or MDA5-2CARD in the cytoplasm. In summary, the results of this study indicate that the BVDV NS4B protein acts as an interferon-β antagonist through inhibiting the MDA5-mediated signal transduction pathway. Our study provides an in-depth understanding of the molecular mechanisms of BVDV evading the host's natural immune response.
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http://dx.doi.org/10.1016/j.virusres.2021.198471DOI Listing
June 2021

The response of tartary buckwheat and 19 bZIP genes to abscisic acid (ABA).

Mol Biol Rep 2021 Jun 7. Epub 2021 Jun 7.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.

Tartary buckwheat is a kind of plant which can be used as medicine as well as edible. Abscisic acid (ABA) signaling plays an important role in the response of plants such as tartary buckwheat to drought and other stress. However, there are not many studies on tartary buckwheat by ABA treatment. In this study, the germination, root length, stoma, and anthocyanin accumulation of tartary buckwheat were all significantly affected by ABA. ABA signaling is important for plants to respond to drought and other stresses, the bZIP gene family is an important member of the ABA signaling pathway. Through the analysis of the origin relationship between tartary buckwheat bZIP family and its related species, 19 bZIP genes in tartary buckwheat were found to be relatively conserved, which laid a foundation for further study of bZIP family. The qRT-PCR results showed that most of the group members were induced by ABA treatment, including 0, 15, 30, 50, 70 µM ABA and 0, 0.5, 2, 4, 8, 16, 24 h ABA treatment. These results suggested that ABA could affect the growth and development of tartary buckwheat, and FtbZIPs might have different functions in the response of tartary buckwheat to drought. This study will be helpful to further analyze the genetic breeding and economic value of tartary buckwheat resistance.
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http://dx.doi.org/10.1007/s11033-021-06449-zDOI Listing
June 2021

Down-Regulation of Associated With Abnormal Ciliogenesis During Embryonic Neurodevelopment Under Inositol Deficiency.

Front Neurol 2021 19;12:579998. Epub 2021 May 19.

Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, China.

The inositol polyphosphate-5-phosphatase E () gene is located on chromosome 9q34.3. The enzyme it encodes mainly hydrolyzes the 5-phosphate groups of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5) P3) and phosphatidylinositol (4,5)-bisphosphate (PtdIns (4,5)P2), which are closely related to ciliogenesis and embryonic neurodevelopment, through mechanisms that are largely unknown. Here we studied the role of gene in ciliogenesis during embryonic neurodevelopment using inositol-deficiency neural tube defects (NTDs) mouse and cell models. Confocal microscopy and scanning electron microscope were used to examine the number and the length of primary cilia. The dynamic changes of expression in embryonic murine brain tissues were observed during Embryonic Day 10.5-13.5 (E 10.5-13.5). Immunohistochemistry, western blot, polymerase chain reaction (PCR) arrays were applied to detect the expression of and cilia-related genes of the embryonic brain tissues in inositol deficiency NTDs mouse. Real-time quantitative PCR (RT-qPCR) was used to validate the candidate genes in cell models. The levels of inositol and PtdIns(3,4) P2 were measured using gas chromatography-mass spectrometry (GC-MS) and enzyme linked immunosorbent assay (ELISA), respectively. Our results showed that the expression levels of gradually decreased in the forebrain tissues of the control embryos, but no stable trend was observed in the inositol deficiency NTDs embryos. expression in inositol deficiency NTDs embryos was significantly decreased compared with the control tissues. The expression levels of gene and the PtdIns (3,4) P2 levels were also significantly decreased in the inositol deficient cell model. A reduced number and length of primary cilia were observed in NIH3T3 cells when inositol deficient. Three important cilia-related genes () were down-regulated significantly in the inositol-deficient NTDs mouse and cell models, and was highly involved in NTDs. In summary, these findings suggested that down-regulation of might be associated with abnormal ciliogenesis during embryonic neurodevelopment, under conditions of inositol deficiency.
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http://dx.doi.org/10.3389/fneur.2021.579998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170399PMC
May 2021

AQP3-mediated H O uptake inhibits LUAD autophagy by inactivating PTEN.

Cancer Sci 2021 Jun 6. Epub 2021 Jun 6.

Department of Thoracic Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, 110042, China.

It is widely accepted that redox reprogramming participates in malignant transformation of lung adenocarcinoma (LUAD). However, the source of excessive reactive oxygen species (ROS) and the downstream signaling regulatory mechanism are complicated and unintelligible. In current study, we newly identified the aquaporin 3 (AQP3) as a LUAD oncogenic factor with capacity to transport exogenous hydrogen peroxide (H O ) and increase intracellular ROS levels. Subsequently, we demonstrated that AQP3 was necessary for the facilitated diffusion of exogenous H O in LUAD cells and that the AQP3-dependent transport of H O accelerated cell growth and inhibited rapamycin-induced autophagy. Mechanistically, AQP3-mediated H O uptake increased intracellular ROS levels to inactivate PTEN and activate the AKT/mTOR pathway subsequently inhibit autophagy and 1promote proliferation in LUAD cells. Finally, we suggested that AQP3 depletion retarded subcutaneous tumorigenesis in vivo and simultaneously decreased ROS levels and promoted autophagy. These findings underscore the importance of AQP3-induced oxidative stress in malignant transformation and suggest a therapeutic target for LUAD.
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http://dx.doi.org/10.1111/cas.15008DOI Listing
June 2021

Suppressing Dehydroisomerization Boosts n-Butane Dehydrogenation with High Butadiene Selectivity.

Chemistry 2021 Jun 5. Epub 2021 Jun 5.

Guangdong University of Technology, Materials and Energy, Guangzhou, 510006, Guangzhou, CHINA.

Butadiene (BD) is a critical raw material in chemical industry, which is conventionally produced from naphtha cracking. The fast-growing demand of BD and the limited oil reserve motivate chemists to develop alternative methods for BD production. Shale gas, which mainly consists of light alkanes, has been considered as cheap raw materials to replace oil for BD production via n -butane direct dehydrogenation ( n -BDH). However, the quest for highly-efficient catalysts for n -BDH is driven by the current drawback of low BD selectivity. Here, we demonstrate a strategy for boosting the selectivity of BD by suppressing dehydroisomerization, an inevitable step in the conventional n -BDH process which largely reduces the selectivity of BD. Detailed investigations show that the addition of alkali-earth metals (e.g., Mg and Ca) into Pt-Ga 2 O 3 /S10 catalysts increases Pt dispersity, suppresses coke deposition and dehydroisomerization, and thus leads to the significant increase of BD selectivity. The optimized catalyst displays an initial BD selectivity of 34.7% at a n -butane conversion of 82.1% at 625 o C, which outperforms the reported catalysts in literatures. This work not only provides efficient catalysts for BD production via n -BDH, but also promotes the researches on catalyst design in heterogeneous catalysis.
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http://dx.doi.org/10.1002/chem.202101087DOI Listing
June 2021

Effects of chromosomal abnormalities on pregnancy outcomes in female undergoing artificial insemination with donor's sperm.

Medicine (Baltimore) 2021 Jun;100(22):e26072

Family Planning Research Institute of Guangdong Province.

Abstract: This study aimed to evaluate the clinical characteristics, pregnancy outcomes and prognostic factors for pregnancy of female with chromosomal abnormalities (CAs) after artificial insemination with donor's sperm (AID) treatment.A retrospective case-control study was analyzed by using the data of 29 female patients with CA and 116 controlled patients with normal karyotype (1:4 ratio) who underwent AID cycles at Guangdong Family Planning Special Hospital from January 2011 to December 2017. In all cases, reproductive histories were collected, and the cytogenetic analysis was performed by Trypsin-Giemsa banding and karyotyping. The embryos were fertilized via intracervical or intrauterine insemination. Clinical characteristic variables were compared.The prevalence of CA was found to be 0.29% in the whole AID population. The live birth rates of CA group and controlled group were 41.4% and 31.0% (P = .29) respectively. Compared to normal karyotype group, patients with CA showed higher rate of primary infertility (93.1% vs 75.9%, P = .049); Multivariate analysis demonstrated that ovarian stimulation (odds ratio, 3.055; 95% confidence interval, 1.421-6.568; P = .004) was associated with adverse pregnancy outcomes in female patients with AID treatment.For the infertility CA patients who were phenotypically normal, AID was a suitable choice, whereas ovarian stimulation results in an improvement in the pregnancy rate.
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http://dx.doi.org/10.1097/MD.0000000000026072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183788PMC
June 2021

A dansyl-based fluorescent probe for sensing Cu in aqueous solution.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 26;261:120009. Epub 2021 May 26.

School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.

A fluorescent probe based on a glycyl-L-tyrosine-modified dansyl derivative (D-GT) is designed and synthesized. D-GT demonstrated great detection performance toward Cu in an aqueous solution. Fluorescence quenching occurred due to the coordination of Cu with D-GT. The sensitive detection of D-GT to Cu was applied in aqueous solution within a wide pH span (6-12). A 1:1 coordinate stoichiometric way and an association constant of 6.47 × 10 M between D-GT and Cu were determined. The measured detection limit for Cu in HEPES buffer solution (10 mM, pH 7.4) was 0.69 μM. The probe displayed an appropriate sensitivity toward Cu in real drinking water samples and living cells, which reveals the potential applications of D-GT in complicated environments.
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http://dx.doi.org/10.1016/j.saa.2021.120009DOI Listing
May 2021

Ultrasensitive Molecular Detection by Imaging of Centimeter-Scale Metasurfaces with a Deterministic Gradient Geometry.

Adv Mater 2021 Jun 4:e2100270. Epub 2021 Jun 4.

Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.

Highly sensitive detection of trace amounts of substances is crucial for broad applications in healthcare, environmental monitoring, antiterrorism, etc., where cost effectiveness and portability are often demanded. Here, an ultrasensitive sensor is reported that can detect an angstrom-thick layer of adsorbed molecules through image acquisition and processing. The sensor features a centimeter-scale plasmonic metasurface with spatially varying geometry, where the light scattering is dependent on both the adsorbed substances and spatial locations. When illuminated with narrowband light (such as from a light emitting diode), the intensity pattern recorded on the metasurface changes with the surface-adsorbed molecules, enabling label-free, sensitive, and spectrometer-free molecular detection. The centimeter-scale size of the sensing area interfaces well with consumer-level imaging sensors on mobile devices without the need for microscopic optics and offers a high signal-to-noise ratio by leveraging the multimillion pixels for noise reduction. It is experimentally demonstrated that a single layer of Al O molecules deposited on the sensor, with a thickness of approximately one angstrom, can be detected by analyzing the images taken of the sensing chip. Furthermore, by integrating the sensor into a microfluidic setup, quantitative detection of BSA/anti-BSA immune complex formation events is demonstrated, which agrees well with the Langmuir isotherm model.
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http://dx.doi.org/10.1002/adma.202100270DOI Listing
June 2021

Exosomes Transport Anti-Human Immunodeficiency Virus Factors from Human Cervical Epithelial Cells to Macrophages.

J Innate Immun 2021 Jun 3:1-11. Epub 2021 Jun 3.

Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, Pennsylvania, USA.

The female reproductive tract (FRT) is a major site of HIV sexual transmission. As the outermost layer of cells in the FRT, the human cervical epithelial cells (HCEs) have direct contact with HIV or infected cells. Our early work showed that supernatant (SN) from TLR3-activated HCEs contain the antiviral factors that could potently inhibit HIV replication in macrophages. However, it remains to be determined how HCEs transport the anti-HIV factors to macrophages. This follow-up study examined the role of exosomes in HCE-mediated anti-HIV activity. We found that TLR3 activation of HCEs resulted in the release of exosomes that contained multiple IFN-stimulated genes (ISGs: ISG56, OAS1, MxA, and Mx2) and the HIV restriction microRNAs (miR-28, miR-29 family members, miR-125b, miR-150, miR-382, miR-223, miR-20a, and miR-198). The depletion of exosomes from SN of TLR3-activated HCEs diminished HCE-mediated anti-HIV activity in macrophages, indicating that HCE-derived exosomes are responsible for transporting the antiviral molecules to macrophages. These in vitro findings suggest a novel antiviral mechanism by which HCEs participate in the FRT innate immunity against HIV infection. Further in vivo studies are necessary in order to develop an exosome-based delivery system for prevention and treatment of HIV infection through sexual transmission.
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http://dx.doi.org/10.1159/000514886DOI Listing
June 2021

circSAMD4A participates in the apoptosis and autophagy of dopaminergic neurons via the miR‑29c‑3p‑mediated AMPK/mTOR pathway in Parkinson's disease.

Mol Med Rep 2021 Jul 3;24(1). Epub 2021 Jun 3.

Department of Neurology, Ningbo No. 6 Hospital, Ningbo, Zhejiang 315040, P.R. China.

Parkinson's disease (PD) can lead to movement injury and cognitive dysfunction. Although advances have been made in attenuating PD, the effect of inhibiting the development of PD remains disappointing. Therefore, the present study aimed at investigating the etiology of Parkinson's disease and developing an alternative therapeutic strategy for patients with PD. A PD mouse model was established using an intraperitoneal injection of 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine hydrochloride (MPTP‑HCl; 30 mg/kg/day for 5 days), and a PD cellular model was established by treating SH‑SY5Y cells with different concentrations of 1‑methyl‑4‑phenylpyridinium (MPP) for 24 h. The expression levels of circular RNA sterile α motif domain containing 4A (circSAMD4A) and microRNA (miR)‑29c‑3p in both midbrain tissues and SH‑SY5Y cells were detected via reverse transcription‑quantitative PCR. The interaction between circSAMD4A and miR‑29c‑3p was verified using a dual‑luciferase reporter experiment. Apoptosis‑, autophagy‑ and 5'AMP‑activated protein kinase (AMPK)/mTOR cascade‑associated proteins in midbrain tissues and SH‑SY5Y cells were detected using western blotting. Furthermore, TUNEL staining and flow cytometry were used to analyze cell apoptosis. It was found that circSAMD4A was upregulated, while miR‑29c‑3p was downregulated in both PD animal and cellular models. Moreover, circSAMD4A directly targeted and negatively regulated miR‑29c‑3p. Further studies identified that circSAMD4A knockdown inhibited MPTP‑ or MPP‑induced apoptosis and autophagy; however, these effects were abolished by an miR‑29c‑3p inhibitor. In addition, circSAMD4A knockdown repressed phosphorylated‑AMPK expression and increased mTOR expression in MPTP‑ or MPP‑induced PD models, the effects of which were reversed by a miR‑29c‑3p inhibitor. Collectively, these results suggested that circSAMD4A participated in the apoptosis and autophagy of dopaminergic neurons by modulating the AMPK/mTOR cascade via miR‑29c‑3p in PD.
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http://dx.doi.org/10.3892/mmr.2021.12179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170871PMC
July 2021

Bikini Incision Increases the Incidence of Lateral Femoral Cutaneous Nerve Injury in Direct Anterior Approach Hip Arthroplasty: A Prospective Ultrasonic, Electrophysiological, and Clinical Study.

J Arthroplasty 2021 May 15. Epub 2021 May 15.

Department of Joint Surgery, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, China.

Background: Lateral femoral cutaneous nerve (LFCN) injury has been widely reported as one of the most common complications of direct anterior approach (DAA) hip arthroplasty. Bikini incision is considered to increase the incidence of this complication.

Methods: A prospective randomized study was conducted after including ninety-nine bikini and ninety-six longitudinal incision DAA cases from May to November 2020. The occurrence of LFCN was examined using ultrasound before and after surgery. The recovery of injury symptoms was evaluated by continuous clinical follow-up until six months, and the patients were treated with mecobalamin and/or celecoxib. Sensory conduction velocity and sensory action potential amplitude of the LFCN were measured after surgery in symptomatic patients.

Results: Eighty five (43.6%), sixty seven (34.4%), and forty three (22.0%) cases of LFCN were of the anterior trunk, posterior trunk, and fan types, respectively, before surgery. All one hundred ninety five patients completed the follow-up period. Fifty-seven patients had symptoms of LFCN injury, including thirty six and twenty one patients in the bikini group and longitudinal group, respectively, with significantly different incidence rates (36.4% and 21.9%, respectively; P < .05). Of these, thirty two (56.1%), thirteen (22.8%), and twelve (21.1%) cases were of the anterior trunk, posterior trunk, and fan types, respectively. Sensory conduction velocity and sensory action potential amplitude significantly decreased after surgery in both groups (P < .05). Seventeen cases showed reduction of symptoms within three months. Forty six cases showed self-recovery within six months and eleven cases showed persistent symptoms at the final follow-up.

Conclusion: Bikini incision DAA hip arthroplasty may increase the incidence of LFCN injury, and the anterior trunk distribution type is most likely to be affected. (Clinical Trial Registration Number: CHICTR2000035107).
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http://dx.doi.org/10.1016/j.arth.2021.05.012DOI Listing
May 2021

Dissipative Particle Dynamics Study on Interfacial Properties of Symmetric Ternary Polymeric Blends.

Polymers (Basel) 2021 May 8;13(9). Epub 2021 May 8.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

We investigated the interfacial properties of symmetric ternary // and // polymeric blends by means of dissipative particle dynamics (DPD) simulations. We systematically analyzed the effects of composition, chain length, and concentration of the copolymers on the interfacial tensions, interfacial widths, and the structures of each polymer component in the blends. Our simulations show that: (i) the efficiency of the copolymers in reducing the interfacial tension is highly dependent on their compositions. The triblock copolymers are more effective in reducing the interfacial tension compared to that of the diblock copolymers at the same chain length and concentration; (ii) the interfacial tension of the blends increases with increases in the triblock copolymer chain length, which indicates that the triblock copolymers with a shorter chain length exhibit a better performance as the compatibilizers compared to that of their counterparts with longer chain lengths; and (iii) elevating the triblock copolymer concentration can promote copolymer enrichment at the center of the interface, which enlarges the width of the phase interfaces and reduces the interfacial tension. These findings illustrate the correlations between the efficiency of copolymer compatibilizers and their detailed molecular parameters.
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http://dx.doi.org/10.3390/polym13091516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125886PMC
May 2021

Fabrication of Magnetic Catalyst FeO-SiO-V and Its Application on Lignin Extraction from Corncob in Deep Eutectic Solvent.

Polymers (Basel) 2021 May 12;13(10). Epub 2021 May 12.

State Key Laboratory of Biobased Material and Green Papermaking, School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

FeO-SiO-V was prepared by deposited HPMoVO on FeO-SiO and employed as a catalyst to extract lignin from corncob in deep eutectic solvent (choline chloride/lactic acid = 1/10). Batch experiments were conducted in an autoclave under the conditions of 500 kPa, 90-130 °C and 15 h, while the dosage of the catalyst was set as a variable. Results indicated that the catalyst could effectively improve the qualities of the lignin, while the characteristics of the lignin showed prominent changes with the participation of the catalyst: the extraction rate increased from 71.65% to 98.13%, the purity was improved from 85.62% to 97.09%, and both the number average molecular weight and the weight average molecular weight also decreased significantly. Besides, the molecular distribution of the lignin achieved from the CC-LA-Fe-Si-V reaction system was found to be more highly concentrated (Polydispersity index = 1.746). Results from 2D NMR HSQC analysis indicated that lignin fractions achieved from the CC-LA-Fe-Si-V system showed distinct destruction involving C-H in guaiacyl units (G), C-H in guaiacyl units (G), and the C-H in γ-hydroxylated β-O-4' substructures, but little changes in the C-H in phenylcoumaran substructures.
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http://dx.doi.org/10.3390/polym13101545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151408PMC
May 2021

The comprehensive measurement method of energy conservation and emission reduction in the whole process of urban sewage treatment based on carbon emission.

Environ Sci Pollut Res Int 2021 Jun 1. Epub 2021 Jun 1.

North China Municipal Engineering Design & Research Institute Co. Ltd., Tianjin, 300074, China.

It is of great significance to establish a carbon emission management system and carbon emission reduction target to put forward emission reduction measures for each subunit of a sewage treatment plant. In this paper, a mathematical model was constructed for calculating carbon emission in the whole sewage treatment system process. Meanwhile, the model calculated the carbon emission changes after upgrading three sewage treatment plants and identified the critical controlling unit. The results showed that the CO produced from electric energy consumption and chemical application was the primary source of carbon emission of wastewater treatment. Raising sewage discharge standards appropriately could effectively reduce the carbon emission generated by each link of the wastewater treatment plant. Further improvement of effluent standards could adversely affect sewage treatment plants in terms of energy, resources, and greenhouse gas emissions. In addition, raising the standard of total phosphorus concentration in the effluent may lead to a corresponding increase in the amount of phosphorus removal agents, as well as an increase in indirect carbon emission, material consumption, and chemical sludge. Therefore, it is necessary to develop sewage treatment technologies that are economical, applicable, energy-saving, and environmental friendly to realize the environmental benefits of carbon emission reduction in sewage treatment and sustainable utilization of energy and resource from wastewater.
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http://dx.doi.org/10.1007/s11356-021-14472-7DOI Listing
June 2021

Synthesis and Characterization of Ultrathin FeTe Nanocrystals.

ACS Omega 2021 Apr 12;6(16):10537-10546. Epub 2021 Apr 12.

Condensed Matter Physics and Materials Science Department, Brookhaven National Laboratory, Upton, New York 11973, United States.

Ultrathin crystals including monolayers have been reported for various transition-metal dichalcogenides (TMDCs) with van der Waals bonds in the crystal structure. Herein, we report a detailed synthesis procedure and characterization of ultrathin iron ditelluride crystals. This material crystallizes in an orthorhombic marcasite crystal structure whose bonding is dominantly covalent and without loosely connected van der Waals (vdW) bonds, making monolayer FeTe synthesis less straightforward than other TMDC monolayer syntheses. The chemical vapor deposition synthesis process described is simple, effective, and results in a range of crystal thicknesses from approximately 400 nm down to the FeTe monolayer.
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http://dx.doi.org/10.1021/acsomega.0c04872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153775PMC
April 2021

A Review of Traditional Chinese Medicine in Treating Renal Interstitial Fibrosis via Endoplasmic Reticulum Stress-Mediated Apoptosis.

Biomed Res Int 2021 15;2021:6667791. Epub 2021 May 15.

Heilongjiang Academy of Chinese Medical Sciences, Harbin 150001, China.

Renal interstitial fibrosis (RIF) is the main pathological manifestation of end-stage renal disease. Recent studies have shown that endoplasmic reticulum (ER) stress is involved in the pathogenesis and development of RIF. Traditional Chinese medicine (TCM), as an effective treatment for kidney diseases, can improve kidney damage by affecting the apoptotic signaling pathway mediated by ER stress. This article reviews the apoptotic pathways mediated by ER stress, including the three major signaling pathways of unfolded protein response, the main functions of the transcription factor C/EBP homologous protein. We also present current research on TCM treatment of RIF, focusing on medicines that regulate ER stress. A new understanding of using TCM to treat kidney disease by regulating ER stress will promote clinical application of Chinese medicine and discovery of new drugs for the treatment of RIF.
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http://dx.doi.org/10.1155/2021/6667791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147530PMC
May 2021

lncRNA WT1-AS is upregulated in osteoporosis and regulates the apoptosis of osteoblasts by interacting with p53.

Exp Ther Med 2021 Jul 9;22(1):734. Epub 2021 May 9.

Cancer Institute Of Hainan Medicail University, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570100, P.R. China.

In cervical cancer, cellular tumor antigen p53 (p53) interacts with long non-coding WT1 antisense RNA (WT1-AS) and this protein serves an important role in osteoporosis. The present study aimed to investigate the role of WT1-AS in osteoporosis. WT1-AS was upregulated in the plasma of patients with osteoporosis and was positively correlated with p53 expression. Altered expression of WT1-AS and p53 separated patients with osteoporosis from healthy controls. Expression levels of WT1-AS and p53 decreased with prolonged treatment. In osteoblasts, WT1-AS overexpression resulted in increased p53 expression, while WT1-AS small interfering RNA (siRNA) silencing resulted in decreased p53 expression. In addition, WT1-AS overexpression resulted in increased apoptosis rate, while WT1-AS siRNA silencing resulted in decreased apoptosis rate in osteoblasts. p53 overexpression attenuated the effects of WT1-AS siRNA silencing on cell apoptosis. Therefore, WT1-AS was upregulated during osteoporosis and regulated the apoptosis of osteoblasts by interacting with p53.
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http://dx.doi.org/10.3892/etm.2021.10166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138279PMC
July 2021

Meta-analysis of Indocyanine Green Fluorescence Imaging-guided Laparoscopic Hepatectomy.

Photodiagnosis Photodyn Ther 2021 May 27:102354. Epub 2021 May 27.

Department of Hepatobiliary Surgery, People's Hospital of Leshan,Sichuan Leshan 614000, China.. Electronic address:

Background: This meta-analysis was conducted to systematically evaluate the short-term efficacy and safety of indocyanine green (ICG) fluorescence imaging-guided laparoscopic hepatectomy.

Methods: A systematic search was conducted of the literature on ICG fluorescence imaging- guided laparoscopic hepatectomy in randomized, semi-randomized controlled trials and observational studies. The found publications and conference papers in English were manually searched and the references included in the literature were traced. The retrieval period was up to February 2021. After evaluating the quality of the included studies, the meta-analysis was conducted using the STATA 15.1 software.

Results: This meta-analysis included 6 studies comprising 417 patients with liver disease. The meta-results showed that compared to the control group, ICG fluorescence imaging-guided laparoscopic hepatectomy can significantly shorten the operative time [weighted mean differences (WMD) = -20.81, 95% CI, -28.02--13.59, P=0.000], reduce intraoperative bleeding [WMD = -108.16, 95% CI, -127.88--88.44, P=0.000], shorten hospital stay [WMD= -1.23,95% CI, -1.50--0.95, P=0.000], and reduce the incidence of postoperative complications [OR = 0.49,95% CI, 0.26-0.91, P=0.025]. There were no differences in blood transfusion, hilar occlusion time, and surgical margin.

Conclusion: The application of ICG fluorescence imaging technology in laparoscopic hepatectomy can effectively reduce the operative time, blood loss, hospital stay and the incidence of postoperative complications. However, more multicenter large-sample randomized controlled trials are needed to further confirm its advantages.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102354DOI Listing
May 2021

Prediction of clinical prognosis in cutaneous melanoma using an immune-related gene pair signature.

Bioengineered 2021 Dec;12(1):1803-1812

Department of Orthopedics, Clinical Medical College, Guizhou Medical University, Guiyang, China.

Cutaneous melanoma (CM) is a malignant and aggressive skin cancer that is the leading cause of skin cancer-related deaths. Increasing evidence shows that immunity plays a vital role in the prognosis of CM. In this study, we developed an immune-related gene pair (IRGP) signature to predict the clinical prognosis of patients with CM. Immune-related genes from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases were selected to construct the IRGPs, and patients with CM in these two cohorts were assigned to low- and high-risk subgroups. Moreover, we investigated the IRGPs and their individualized prognostic signatures using Kaplan-Meier survival analysis, univariate and multivariate Cox analyses, and analysis of immune cell infiltration in CM. A 41-IRGP signature was constructed from 2498 immune genes that could significantly predict the overall survival of patients with CM in both the TCGA and GEO cohorts. Immune infiltration analysis indicated that several immune cells, especially M1 macrophages and activated CD4 T cells, were significantly associated with the prognostic effect of the IRGP signature in patients with CM. Overall, the IRGP signature constructed in this study was useful for determining the prognosis of patients with CM and for providing further understanding of CM immunotherapy.
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http://dx.doi.org/10.1080/21655979.2021.1924556DOI Listing
December 2021

Electrically programmable adhesive hydrogels for climbing robots.

Sci Robot 2021 Apr;6(53)

College of Materials, Xiamen University, Xiamen 361005, People's Republic of China.

Although there have been notable advances in adhesive materials, the ability to program attaching and detaching behavior in these materials remains a challenge. Here, we report a borate ester polymer hydrogel that can rapidly switch between adhesive and nonadhesive states in response to a mild electrical stimulus (voltages between 3.0 and 4.5 V). This behavior is achieved by controlling the exposure and shielding of the catechol group through water electrolysis-induced reversible cleavage and reformation of the borate ester moiety. By switching the electric field direction, the hydrogel can repeatedly attach to and detach from various surfaces with a response time as low as 1 s. This programmable attaching/detaching strategy provides an alternative approach for robot climbing. The hydrogel is simply pasted onto the moving parts of climbing robots without complicated engineering and morphological designs. Using our hydrogel as feet and wheels, the tethered walking robots and wheeled robots can climb on both vertical and inverted conductive substrates (i.e., moving upside down) such as stainless steel and copper. Our study establishes an effective route for the design of smart polymer adhesives that are applicable in intelligent devices and an electrochemical strategy to regulate the adhesion.
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http://dx.doi.org/10.1126/scirobotics.abe1858DOI Listing
April 2021

cfDNApipe: A comprehensive quality control and analysis pipeline for cell-free DNA high-throughput sequencing data.

Bioinformatics 2021 May 27. Epub 2021 May 27.

Ministry of Education Key Laboratory of Bioinformatics; Center for Synthetic and Systems Biology; Bioinformatics Division, Beijing National Research Center for Information Science and Technology; Department of Automation.

Motivation: Cell-free DNA (cfDNA) is gaining substantial attention from both biological and clinical fields as a promising marker for liquid biopsy. Many aspects of disease-related features have been discovered from cfDNA high-throughput sequencing (HTS) data. However, there is still a lack of integrative and systematic tools for cfDNA HTS data analysis and quality control (QC).

Results: Here, we propose cfDNApipe, an easy-to-use and systematic python package for cfDNA whole-genome sequencing (WGS) and whole-genome bisulfite sequencing (WGBS) data analysis. It covers the entire analysis pipeline for the cfDNA data, including raw sequencing data processing, QC and sophisticated statistical analysis such as detecting copy number variations (CNVs), differentially methylated regions (DMRs) and DNA fragment size alterations. cfDNApipe provides one-command-line-execution pipelines and flexible application programming interfaces for customized analysis.

Availability: https://xwanglabthu.github.io/cfDNApipe/.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab413DOI Listing
May 2021

A point-of-care chemiluminescence immunoassay for pepsinogen I enables large-scale community health screening.

Anal Bioanal Chem 2021 May 26. Epub 2021 May 26.

Department of Laboratory Medicine, Guangzhou First People's Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou, 510180, China.

Pepsinogen I (PGI) can reflect the morphology and function of the gastric mucosa. Accordingly, the large-scale community health screening of PGI can dramatically increase the early diagnosis rate of gastric cancer. However, PGI testing can only be carried out in comprehensive hospitals and health examination centers. To ameliorate this issue, a point-of-care chemiluminescent immunoassay for PGI was developed in a fully automated miniaturized instrument. This instrument was especially developed for health check-ups in the grassroots communities; its volume of which is only 0.18 m. Critically, the entire detection process for a single sample only requires 20 min, and the samples can be loaded continuously, making the method suitable for high-throughput analysis. The assay displayed an excellent detection limit of 0.048 ng/mL with a broad detection range of 0-200 ng/mL. Furthermore, this assay exhibited high sensitivity and specificity, had low intra- and inter-assay coefficients of variation (<10%), and was not affected after storage at 37 °C for 7 days. The assay was used to detect PGI in 95 clinical serum samples, and the results were highly correlated with those that were clinically tested (correlation coefficient, R = 0.998). Hence, the method established in this work has great application value and can be broadly applied for the large-scale screening of gastric cancer in resource-limited areas.
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http://dx.doi.org/10.1007/s00216-021-03412-6DOI Listing
May 2021