Publications by authors named "Yu Lin"

1,113 Publications

  • Page 1 of 1

Association of cardiovascular disease with 30-day hospital readmission in Chinese patients receiving maintenance dialysis.

Ann Transl Med 2021 Apr;9(8):617

Department of Medicine, Peking University First Hospital, Beijing, China.

Background: Previous studies have shown cardiovascular disease (CVD) to be a risk factor in the prediction of 30-day hospital readmission among patients receiving dialysis. However, studies of Asian populations are limited. In the present study, we examined the association between CVD and 30-day hospital readmission in Chinese patients receiving maintenance dialysis.

Methods: Patients receiving maintenance dialysis were identified by searching a national claims database, the China Health Insurance Research Association (CHIRA) database, using the International Classification of Diseases revision 10 (ICD-10) and items of medical service claims. Patients aged ≥18 years who were discharged after index hospitalization between January 2015 and December 2015 were included in our retrospective analysis. CVD-related diagnoses were divided into three categories: coronary heart disease (CHD), heart failure (HF), and stroke. Thirty-day hospital readmission was defined as any hospital readmission within the 30 days following discharge. Logistic regression models adjusted for logit of propensity scores (PS) were used to assess the association of CVD with 30-day hospital readmission.

Results: Of 4,700 patients receiving dialysis, the 30-day hospital readmission rate was 10.4%. Compared with patients without CVD, there was an increased risk of 30-day hospital readmission among maintenance dialysis patients with total CVD [odds ratio (OR): 1.33, 95% confidence interval (CI): 1.06-1.66]. Patients with HF (OR: 1.77, CI: 1.27-2.47) and stroke (OR: 2.14, 95% CI: 1.53-2.98) had a greater risk of 30-day hospital readmission. The fully adjusted OR of CHD for the risk of 30-day hospital readmission was 1.22 (95% CI: 0.97-1.55).

Conclusions: CVDs, especially stroke and HF, are independent predictors of 30-day hospital readmission in Chinese patients receiving dialysis, and could help to guide interventions to improve the quality of care for these patients.
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http://dx.doi.org/10.21037/atm-20-2367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106029PMC
April 2021

IL-17-producing γδT cells ameliorate intestinal acute graft-versus-host disease by recruitment of Gr-1CD11b myeloid-derived suppressor cells.

Bone Marrow Transplant 2021 May 13. Epub 2021 May 13.

Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Acute graft-versus-host disease (aGVHD) is the most severe complication and the major cause of morbidity and mortality in patients who have received hematopoietic stem cell transplantation. Inflammation mediated by donor T cells and neighboring recipient cells is considered to be responsible for intestinal aGVHD. Interleukin (IL)-17A-producing γδT (γδT17) cells have been investigated as key players in cancer, immunity, and inflammatory responses because of their phenotypic plasticity, memory-like activity, and unique migratory features. However, the precise roles and underlying mechanisms of γδT17 cells in aGVHD immunopathogenesis remain elusive. Here, we found that γδT17 cells constituted the major resident γδT population in the intestinal lamina propria of aGVHD mice. Adoptive infusion of induced γδT17 cells markedly attenuated murine aGVHD and increased infiltration of Gr-1CD11b myeloid-derived suppressor cells (MDSCs) into the inflammatory intestine, and did not affect graft-versus-leukemia(GVL) effect. Further experiments indicated that γδT17 cells enhanced both migration ability and immunosuppressive activity of MDSCs. γδT17 cell-mediated protection in aGVHD was blunted by depletion of IL-17A or MDSCs. Our study clarifies an immune pathway where γδT17 cells play a protective role in murine aGVHD by recruiting MDSCs to the inflammatory intestine.
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http://dx.doi.org/10.1038/s41409-021-01299-6DOI Listing
May 2021

Rapidly Progressing Fatal Left Ventricular Pseudoaneurysm After Acute Myocardial Infarction - A Case Report of Delayed Diagnosis.

Heart Surg Forum 2021 Apr 29;24(2):E414-E417. Epub 2021 Apr 29.

Department of Cardiothoracic Surgery, Guangdong Provincial Hospital of Chinese Medicine, People's Republic of China.

Left ventricular pseudoaneurysm (LVPA) is a rare complication of acute myocardial infarction (MI). As pseudoaneurysm is contained by the pericardium alone without involvement of myocardial tissue, LVPA are more prone to rupture and hence necessitates surgical intervention. We report a case of a 60-year-old man with acute MI due to a three-way occlusion in the coronary arteries. An emergency transthoracic echocardiogram (TTE) on the 11th day after the MI showed a small ventricular aneurysm, which was probably a late complication of the acute MI episode. A repeat TTE on the 26th day of the MI episode revealed a rapidly progressing LVPA. Emergency heart surgery was planned, but the patient died due to LVPA rupture. This case illustrates timely diagnosis and corrective surgery are key to saving patients from fatal LVPAs.
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http://dx.doi.org/10.1532/hsf.3679DOI Listing
April 2021

Comparison of Two Major Staging Systems in Predicting Survival and Recommendation of Postoperative Radiotherapy Based on the 11th Japanese Classification for Esophageal Carcinoma After Curative Resection: A Propensity Score-Matched Analysis.

Ann Surg Oncol 2021 May 9. Epub 2021 May 9.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: The aim of this study was to compare the prognostic predictive power of the 11th Japan Esophageal Society (JES) staging system with the 8th edition of the American Joint Committee on Cancer (AJCC) staging system in patients with thoracic esophageal squamous cell carcinoma (TESCC), and to estimate the survival benefits of postoperative radiotherapy (PORT) based on a substage of the JES staging system.

Methods: Area under the curve (AUC) values of the receiver operating characteristic curve were calculated to evaluate prognostic efficacy. Propensity score matching (PSM) analysis was conducted to balance the two groups (surgery only [S group] or surgery plus PORT [S+RT group]) across substages of the 11th JES staging system according to independent prognostic factors for overall survival (OS) identified using Cox proportional hazards regression.

Results: A total of 2960 patients were eligible. The 5-year OS AUC for the 8th AJCC staging system was significantly higher than that for the 11th JES staging system (0.701 vs. 0.675, p < 0.001). Before PSM, PORT significantly improved 5-year OS rates for patients in stage III and IVA by 9.1% (p < 0.001) and 21.1% (p < 0.001), respectively. After PSM, the 5-year OS rates in stage II, III, and IVA of the S+RT group were significantly higher than those in the S group (70.9%, 39.7%, and 35.1% vs. 57.8%, 27.2%, and 10.3%, respectively; p < 0.001).

Conclusion: The 11th JES staging system was less capable of predicting prognosis than the 8th AJCC staging system and patients in stage III of the JES staging system were highly recommended to undergo PORT.
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http://dx.doi.org/10.1245/s10434-021-10046-6DOI Listing
May 2021

Effect of Acute Psychological Stress on Motion-in-Depth Perception: An Event-Related Potential Study.

Adv Cogn Psychol 2020 16;16(4):353-362. Epub 2020 Dec 16.

Department of Psychology, Wuhan Institute of Physical Education, Wuhan, China2.

The present study explored the intrinsic event-related potential (ERP) features of the effects of acute psychological stress on the processing of motion-in-depth perception using a dual-task paradigm. After a mental arithmetic task was used to induce acute psychological stress, a collision task was used to evaluate motion-in-depth perception. The error value and average amplitude of late slow waves (SW) were significantly larger for the earlier colliding spheres' than for the later colliding spheres. The P1 peak latency in the left occipital region was significantly shorter than that of the right occipital region in the motion-in-depth perception task. Compared to the control condition, the estimated value of residual time-to-collision and error value were significantly reduced, and the N1 peak amplitude and the SW averaged amplitude were significantly increased in the stress condition. Longer motion-in-depth time improved discrimination accuracy and decreased the investment of cognitive resources. Acute psychological stress increased behavioral performance and enhanced attention resources on the motion-in-depth perception task together with greater investment of cognitive resources.
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http://dx.doi.org/10.5709/acp-0309-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085683PMC
December 2020

Improving metagenomic binning results with overlapped bins using assembly graphs.

Algorithms Mol Biol 2021 May 4;16(1). Epub 2021 May 4.

School of Computing, College of Engineering and Computer Science, Australian National University, Canberra, Australia.

Background: Metagenomic sequencing allows us to study the structure, diversity and ecology in microbial communities without the necessity of obtaining pure cultures. In many metagenomics studies, the reads obtained from metagenomics sequencing are first assembled into longer contigs and these contigs are then binned into clusters of contigs where contigs in a cluster are expected to come from the same species. As different species may share common sequences in their genomes, one assembled contig may belong to multiple species. However, existing tools for binning contigs only support non-overlapped binning, i.e., each contig is assigned to at most one bin (species).

Results: In this paper, we introduce GraphBin2 which refines the binning results obtained from existing tools and, more importantly, is able to assign contigs to multiple bins. GraphBin2 uses the connectivity and coverage information from assembly graphs to adjust existing binning results on contigs and to infer contigs shared by multiple species. Experimental results on both simulated and real datasets demonstrate that GraphBin2 not only improves binning results of existing tools but also supports to assign contigs to multiple bins.

Conclusion: GraphBin2 incorporates the coverage information into the assembly graph to refine the binning results obtained from existing binning tools. GraphBin2 also enables the detection of contigs that may belong to multiple species. We show that GraphBin2 outperforms its predecessor GraphBin on both simulated and real datasets. GraphBin2 is freely available at https://github.com/Vini2/GraphBin2 .
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http://dx.doi.org/10.1186/s13015-021-00185-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097841PMC
May 2021

Decision aids for prenatal testing: A systematic review and meta-analysis.

J Adv Nurs 2021 May 3. Epub 2021 May 3.

School of Nursing, Jilin University, Changchun, Jilin Province, China.

Aims: To analyse the effect of decision aids (DAs) used by pregnant women on prenatal testing decisions.

Design: Systematic review and meta-analysis.

Data Resources: We searched Embase, PubMed, Web of Science and the Cochrane Central Library ending October 2020.

Review Methods: Papers were selected for analysis in accordance with the PRISMA guidelines. The meta-analysis was carried out using Review Manager 5.3 software. The quality of the studies was assessed using the risk of bias tool recommended by the Cochrane Handbook. The result is knowledge, decision conflict, anxiety and other secondary outcomes.

Results: A total of 18 studies were included in the systematic review and meta-analysis. Comprehensive analysis showed that DAs could significantly improve knowledge and decision-making satisfaction, reduce decision conflict, increase the proportion of women who make informed choice and had no influence on anxiety and decision regret.

Conclusions: This article systematically reviewed the positive effect of DAs on the decision-making of pregnant women facing prenatal testing. In the future, nurses should be encouraged to develop DAs in accordance with strict standards and apply them to pregnant women of different backgrounds.

Impact: There is a growing consensus that health care should be patient-centred, and the values and preferences of pregnant women who undergo prenatal testing need to be incorporated into the clinical decision-making process. This review reports that the use of DAs can increase pregnant women's chances of participating in prenatal testing decisions and may improve the quality of their decision-making. It also provides information on the role and practice of nurses in promoting evidence-based prenatal testing for DAs.
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http://dx.doi.org/10.1111/jan.14875DOI Listing
May 2021

A Panel of Glycopeptides as Candidate Biomarkers for Early Diagnosis of NASH Hepatocellular Carcinoma Using a Stepped HCD Method and PRM Evaluation.

J Proteome Res 2021 Apr 30. Epub 2021 Apr 30.

Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, United States.

Changes in N-glycosylation on specific peptide sites of serum proteins have been investigated as potential markers for diagnosis of nonalcoholic steatohepatitis (NASH)-related HCC. To accomplish this work, a novel workflow involving broad-scale marker discovery in serum followed by targeted marker evaluation of these glycopeptides were combined. The workflow involved an LC-Stepped HCD-DDA-MS/MS method coupled with offline peptide fractionation for large-scale identification of -glycopeptides directly from pooled serum samples (each = 10) as well as differential determination of N-glycosylation changes between disease states. We then evaluated several potentially diagnostic N-glycopeptides among 78 individual patient samples (40 cirrhosis, 28 early stage NASH HCC, and 10 late-stage NASH HCC) by LC-Stepped HCD-PRM-MS/MS to quantitatively analyze 65 targeted glycopeptides from 7 glycoproteins. Of these targets, we found site-specific -glycopeptides nGSLFAFR_HexNAc(4)Hex(5)NeuAc(2) and nISDGFDGIPDNVDAALALPAHSYSGR_HexNAc(5)Hex(6)Fuc(1)NeuAc(3) from VTNC were significantly increased comparing samples from patients with NASH cirrhosis and NASH HCC ( < 0.05). When combining results of these 2 glycopeptides with AFP, the ROC curve analysis demonstrated the AUC value increased to 0.834 (95% CI, 0.748-0.921) and 0.847 (95% CI, 0.766-0.932), respectively, as compared to that of AFP alone (AUC = 0.791, 95% CI, 0.690-0.892). These 2 glycopeptides may serve as potential biomarkers for early HCC diagnosis in patients with NASH related cirrhosis.
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http://dx.doi.org/10.1021/acs.jproteome.1c00175DOI Listing
April 2021

Use of housefly (Musca domestica L.) larvae to bioconversion food waste for animal nutrition and organic fertilizer.

Environ Sci Pollut Res Int 2021 Apr 30. Epub 2021 Apr 30.

College of Environment, Sichuan Agricultural University, Chengdu, 611130, China.

In this study, a mixed-level orthogonal array design was employed for the optimum conditions of breeding housefly larvae by food waste. The results showed that the effects of these factors on the weight of 50 larvae, larvae yield, and crude protein content were the culture substrate ratio > the breeding density > the feeding mode. The optimum conditions for the housefly larvae to convert food waste were as follows: culture substrates ratio 1:3, breeding density 10.0 g/kg, and all substance added on the first day. The optimum food waste mass reduction rate was 79.1-83.6%. The value of the essential amino acids (Eaa)/ the total amino acids (Taa) (45.1%) and E/the nonessential amino acid (Naa) values (0.83%) in the housefly larvae products met the Food and Agricultural Organization (FAO) requirements for feed protein. The crude fat content (30.1 ± 1.18%) was higher than of the housefly larvae after bioconversion of pig manure (22.0%) and the fish meal standard of China. The contents of total nutrients (N+P+K ≥ 5.5%) and heavy metals (Pb ≤ 0.40 mg/kg, Cr ≤ 1.50 mg/kg, Cd ≤ 0.40 mg/kg) in the residues of this study met the Chinese standard for organic fertilizer. Tilapia raised with the dried housefly larvae showed the best growth performance and nutrient concentrations in the experiment groups (p < 0.05). Moreover, the trace elements concentration in tilapia raised with the four kinds of feeds complied with the maximum levels of contaminants in foods in both China and WHO. These findings show that the housefly larvae products that converted food waste are suitable for use in the production of fish feed.
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http://dx.doi.org/10.1007/s11356-021-14118-8DOI Listing
April 2021

[Structure and species diversity of community in Dawei Mountain of Hunan, China.]

Ying Yong Sheng Tai Xue Bao 2021 Apr;32(4):1201-1212

Forestry Bureau of Anhua County, Anhua 413500, Hunan, China.

To provide a scientific basis for the conservation and exploitation of wild species in Dawei Mountain, we investigated the community characteristics and species diversity of species. The results showed that there were four communities, . , . , . and . , in Dawei Mountain. Phaenerophyte in the life form and pantropical elements in the local flora were both dominant. The shrub layer had higher species diversity than that of the arbor layer. Species diversity of those four communities was following the order of . , . , . and . . The community structure was relatively stable when young and mature individuals of predominated. The ground dia-meter class structure indicated that the . population was a growing population with a typical pyramid structure. The . community was mono-dominated by . , with . and . as the important companion species. Those three species would be replaced by other coniferous and broadleaved species due to the shortage of natural regeneration.
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http://dx.doi.org/10.13287/j.1001-9332.202104.006DOI Listing
April 2021

Injectable Thermogel Generated by the "Block Blend" Strategy as a Biomaterial for Endoscopic Submucosal Dissection.

ACS Appl Mater Interfaces 2021 May 21;13(17):19778-19792. Epub 2021 Apr 21.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China.

Endoscopic submucosal dissection is an established method for the removal of early cancers and large lesions from the gastrointestinal tract but is faced with the risk of perforation. To decrease this risk, a submucosal fluid cushion (SFC) is needed clinically by submucosal injection of saline and so on to lift and separate the lesion from the muscular layer. Some materials have been tried as the SFC so far with disadvantages. Here, we proposed a thermogel generated by the "block blend" strategy as an SFC. This system was composed of two amphiphilic block copolymers in water, so it was called a "block blend". We synthesized two non-thermogellable copolymers poly(d,l-lactide--glycolide)--poly(ethylene glycol)--poly(d,l-lactide--glycolide) and blended them in water to achieve a sol-gel transition upon heating in both pure water and physiological saline. We explored the internal structure of the resultant thermogel with transmission electron microscopy, three-dimensional light scattering, C NMR, fluorescence resonance energy transfer, and rheological measurements, which indicated a percolated micelle network. The biosafety of the synthesized copolymer was preliminarily confirmed . The main necessary functions as an SFC, namely, injectability of a sol and the maintained mucosal elevation as a gel after injection, were verified . This study has revealed the internal structure of the block blend thermogel and illustrated its potential application as a biomaterial. This work might be stimulating for investigations and applications of intelligent materials with both injectability and thermogellability of tunable phase-transition temperatures.
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http://dx.doi.org/10.1021/acsami.1c03849DOI Listing
May 2021

Application of UV radiation for in-situ Cr(VI) reduction from contaminated soil with electrokinetic remediation.

J Hazard Mater 2021 Apr 6;416:125806. Epub 2021 Apr 6.

State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China; College of Resource and Safety Engineering, Chongqing University, Chongqing, 400044, China. Electronic address:

Restoring hexavalent chromium (Cr(VI)) to trivalent chromium (Cr(III)) from contaminated soil is a cost-effective alternative for attenuating Cr(VI) toxicity to the ecosystem. A new electrokinetic remediation (EKR) system with UV light was explored to overcome an energy barrier to catalyze Cr(VI) reduction from the surface soil near the anodic reservoir. Natural organic matters and minerals from the contaminated soil acted as electron donors and catalysts for Cr(VI) photo-reduction and no additional chemical reagent. There was almost no residual Cr(VI) in anolyte after UV/EKR compared with the conventional EKR. The reduction improved the efficiency of EKR in the soil near the anodic reservoir by dropped the Cr(VI) negative mass flux caused by electroosmosis advection and concentration diffusion. The pathways of Cr(VI) photo-reduction are possibly dominated by ligand-to-metal charge transfer, i.e., photocatalytic cyclic reduction by Fe(III)/Fe(II) complexes on the surface of the minerals and in soil pore fluid and the photo-induced decomposition of chromate ester. It is concluded that UV/EKR is a clean, efficient, and low-cost method for remediation of Cr(VI)-contaminated soil.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125806DOI Listing
April 2021

Differential circular RNA expression profiles in umbilical cord blood exosomes from preeclampsia patients.

BMC Pregnancy Childbirth 2021 Apr 15;21(1):303. Epub 2021 Apr 15.

Department of Neonatology, The Affiliated Wuxi Children's Hospital of Nanjing Medical University, Wuxi, 214023, China.

Background: Exosomal circular RNAs (circRNAs) are emerging as important regulators of physiological development and disease pathogenesis. However, the roles of exosomal circRNAs from umbilical cord blood in preeclampsia (PE) occurrence remains poorly understood.

Methods: We used microarray technology to establish the differential circRNA expression profiles in umbilical cord blood exosomes from PE patients compared with normal controls. Bioinformatics analysis was conducted to further predict the potential effects of the differentially expressed circRNAs and their interactions with miRNAs.

Results: According to the microarray data, we identified 143 significantly up-regulated circRNAs and 161 significantly down-regulated circRNAs in umbilical cord blood exosomes of PE patients compared with controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses showed that circRNA parental genes involved in the regulation of metabolic process, trophoblast growth and invasion were significantly enriched, which play important roles in PE development. Moreover, pathway network was constructed to reveal the key pathways in PE, such as PI3K-Akt signaling pathway. Further circRNA/miRNA interactions analysis demonstrated that most exosomal circRNAs had miRNA binding sites, and some miRNAs were associated with PE.

Conclusions: Our results highlight the importance of exosomal circRNAs in the pathogenesis of PE and lay a foundation for extensive studies on the role of exosomal circRNAs in PE development.
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http://dx.doi.org/10.1186/s12884-021-03777-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051099PMC
April 2021

Non-invasive assessment of heterogeneity of gliomas using diffusion and perfusion MRI: correlation with spatially co-registered PET.

Acta Radiol 2021 Apr 15:2841851211006913. Epub 2021 Apr 15.

Department of Nuclear Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, PR China.

Background: Heterogeneity of gliomas challenges the neuronavigated biopsy and oncological therapy. Diffusion and perfusion magnetic resonance imaging (MRI) can reveal the cellular and hemodynamic heterogeneity of tumors. Integrated positron emission tomography (PET)/MRI is expected to be a non-invasive imaging approach to characterizing glioma.

Purpose: To evaluate the value of apparent diffusion coefficient (ADC), cerebral blood volume (CBV), and spatially co-registered maximal standard uptake value (SUV) for tissue characterization and glioma grading.

Material And Methods: Thirty-seven consecutive patients with pathologically confirmed gliomas were retrospectively investigated. The relative minimum ADC (rADC), relative maximal ADC (rADC), relative maximal rCBV (rCBV), the relative minimum rCBV (rCBV), and the corresponding relative SUVmax (rSUV) were measured. The paired test was used to compare the quantitative parameters between different regions to clarify tumor heterogeneity. Imaging parameters between WHO grade IV and grade II/III gliomas were compared by -test. The diagnostic efficiency of multiparametric PET/MRI was analyzed by receiver operating characteristic (ROC) curve.

Results: The values of rSUV were significantly different between maximal diffusion/perfusion area and minimum diffusion/perfusion area ( < 0.001/ < 0.001) within tumor. The values of rADC ( < 0.001), rCBV ( = 0.002), and corresponding rSUV ( = 0.001/ < 0.001) could be used for grading gliomas. The areas under the ROC curves of rSUV defined by rADC and rCBV were 0.89 and 0.91, respectively.

Conclusion: Diffusion and perfusion MRI can detect glioma heterogeneity with excellent molecular imaging correlations. Regions with rCBV suggest tissues with the highest metabolism and malignancy for guiding glioma grading and tissue sampling.
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http://dx.doi.org/10.1177/02841851211006913DOI Listing
April 2021

PEG-based thermosensitive and biodegradable hydrogels.

Acta Biomater 2021 Apr 20. Epub 2021 Apr 20.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Fudan University, Shanghai 200438, China; Zhuhai Fudan Innovation Institute, Zhuhai, Guangdong 519000, China.

Injectable thermosensitive hydrogels are free-flowing polymer solutions at low or room temperature, making them easy to encapsulate the therapeutic payload or cells via simply mixing. Upon injection into the body, in situ forming hydrogels triggered by body temperature can act as drug-releasing reservoirs or cell-growing scaffolds. Finally, the hydrogels are eliminated from the administration sites after they accomplish their missions as depots or scaffolds. This review outlines the recent progress of poly(ethylene glycol) (PEG)-based biodegradable thermosensitive hydrogels, especially those composed of PEG-polyester copolymers, PEG-polypeptide copolymers and poly(organophosphazene)s. The material design, performance regulation, thermogelation and degradation mechanisms, and corresponding applications in the biomedical field are summarized and discussed. A perspective on the future thermosensitive hydrogels is also highlighted. STATEMENT OF SIGNIFICANCE: Thermosensitive hydrogels undergoing reversible sol-to-gel phase transitions in response to temperature variations are a class of promising biomaterials that can serve as minimally invasive injectable systems for various biomedical applications. Hydrophilic PEG is a main component in the design and fabrication of thermoresponsive hydrogels due to its excellent biocompatibility. By incorporating hydrophobic segments, such as polyesters and polypeptides, into PEG-based systems, biodegradable and thermosensitive hydrogels with adjustable properties in vitro and in vivo have been developed and have recently become a research hotspot of biomaterials. The summary and discussion on molecular design, performance regulation, thermogelation and degradation mechanisms, and biomedical applications of PEG-based thermosensitive hydrogels may offer a demonstration of blueprint for designing new thermogelling systems and expanding their application scope.
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http://dx.doi.org/10.1016/j.actbio.2021.04.009DOI Listing
April 2021

Complexity of species causing take-all root rot of St. Augustinegrass in Texas.

Mycologia 2021 May-Jun;113(3):599-611. Epub 2021 Apr 14.

Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843.

var. () has been the etiological agent of take-all root rot (TARR) in St. Augustinegrass () and root decline of the other warm-season turfgrasses. Seventy-five isolates were obtained from St. Augustinegrass in central and east Texas. Evaluation of colony morphologies on potato dextrose agar (PDA) within 2 wk and follow-up multilocus phylogenic analyses revealed three phenotypic groups associated with different species: (i) , highly melanized with round colony formation; (ii) , none to slightly melanized with round colony formation; and (iii) , highly melanized with irregular colony formation. Further examination with representative isolates from each group revealed that their phenotypic characterizations supported the distinctive genetic groups within associated with St. Augustinegrass TARR. isolates grew faster at warmer temperature (30 C) than or . Pathogenicity assays using rice seedlings indicated that was more aggressive in disease symptom development than or . A multilocus phylogeny reconstruction supported that most of isolates tested in this study were separated into three phylogenetically distinct groups: , and . graminicola. The resolution of intravarietal complexities of causal fungi of TARR is important for proper diagnostics and management strategies for TARR in St. Augustinegrass and other root-decline diseases in warm-season turfgrasses.
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http://dx.doi.org/10.1080/00275514.2021.1881735DOI Listing
April 2021

Principal Component Analysis of the Running Ground Reaction Forces With Different Speeds.

Front Bioeng Biotechnol 2021 25;9:629809. Epub 2021 Mar 25.

Faculty of Sports Science, Ningbo University, Ningbo, China.

Ground reaction force (GRF) is a key metric in biomechanical research, including parameters of loading rate (LR), first impact peak, second impact peak, and transient between first and second impact peaks in heel strike runners. The GRFs vary over time during stance. This study was aimed to investigate the variances of GRFs in rearfoot striking runners across incremental speeds. Thirty female and male runners joined the running tests on the instrumented treadmill with speeds of 2.7, 3.0, 3.3, and 3.7 m/s. The discrete parameters of vertical average loading rate in the current study are consistent with the literature findings. The principal component analysis was modeled to investigate the main variances (95%) in the GRFs over stance. The females varied in the magnitude of braking and propulsive forces (PC1, 84.93%), whereas the male runners varied in the timing of propulsion (PC1, 53.38%). The female runners dominantly varied in the transient between the first and second peaks of vertical GRF (PC1, 36.52%) and LR (PC2, 33.76%), whereas the males variated in the LR and second peak of vertical GRF (PC1, 78.69%). Knowledge reported in the current study suggested the difference of the magnitude and patterns of GRF between male and female runners across different speeds. These findings may have implications for the prevention of sex-specific running-related injuries and could be integrated with wearable signals for the in-field prediction and estimation of impact loadings and GRFs.
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http://dx.doi.org/10.3389/fbioe.2021.629809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026898PMC
March 2021

Calcium supplementation relieves high-fat diet-induced liver steatosis by reducing energy metabolism and promoting lipolysis.

J Nutr Biochem 2021 Apr 7;94:108645. Epub 2021 Apr 7.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, P.R. China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD) is a chronic disease affecting the health of many people worldwide. Previous studies have shown that dietary calcium supplementation may alleviate NAFLD, but the underlying mechanism is not clear. In this study investigating the effect of calcium on hepatic lipid metabolism, 8-week-old male C57BL/6J mice were divided into four groups (n = 6): (1) mice given a normal chow containing 0.5% calcium (CN0.5), (2) mice given a normal chow containing 1.2% calcium (CN1.2), (3) mice given a high-fat diet (HFD) containing 0.5% calcium (HFD0.5), and (4) mice fed a HFD containing 1.2% calcium (HFD1.2). To understand the underlying mechanism, cells were treated with oleic acid and palmitic acid to mimic the HFD conditions in vitro. The results showed that calcium alleviated the increase in triglyceride accumulation induced by oleic acid and/or palmitic acid in HepG2, AML12, and primary hepatocyte cells. Our data demonstrated that calcium supplementation alleviated HFD-induced hepatic steatosis through increased liver lipase activity, proving calcium is involved in the regulation of hepatic lipid metabolism. Moreover, calcium also increased the level of glycogen in the liver, and at the same time had the effect of reducing glycolysis and promoting glucose absorption. Calcium addition increased calcium levels in the mitochondria and cytoplasm. Taken together, we concluded that calcium supplementation could relieve HFD-induced hepatic steatosis by changing energy metabolism and lipase activity.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108645DOI Listing
April 2021

The Prevalence of Diabetic Microvascular Complications in China and the USA.

Curr Diab Rep 2021 Apr 9;21(6):16. Epub 2021 Apr 9.

Renal Division, Department of Medicine, Peking University First Hospital, 8 Xishiku Street, Xicheng District, Beijing, 100034, China.

Purpose Of Review: Diabetes can lead to development of devastating microvascular complications, such as nephropathy, retinopathy, and peripheral sensory and autonomic neuropathy. While China and the USA both face the threat of this major public health challenge, the literature is limited in describing similarities and differences in the prevalence, and risk factors for the development, of diabetic microvascular complications between these two countries.

Recent Findings: The current review discusses the following: (1) the most recent evidence on prevalence of diabetic microvascular complications in China and the USA (including downtrends of diabetes retinopathy and neuropathy in the USA); (2) differences in patient risk factors of these complications; (3) challenges and current knowledge gaps (such as lacking national epidemiological data of diabetic complications in China); and (4) potential future clinical and research opportunities (including needs in diabetes evaluation and management in remote areas and standardization of methods in evaluating diabetic complications across countries). Diabetic microvascular complications remain to be health threats in both China and the USA. Further investigations are needed for comprehensive understanding and effect prevention and management of these complications.
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http://dx.doi.org/10.1007/s11892-021-01387-3DOI Listing
April 2021

A novel platform for discovery of differentially expressed microRNAs in patients with repeated implantation failure.

Fertil Steril 2021 Apr 3. Epub 2021 Apr 3.

Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:

Objective: To identify predictor microRNAs (miRNAs) from patients with repeated implantation failure (RIF).

Design: Systemic analysis of miRNA profiles from the endometrium of patients undergoing in vitro fertilization (IVF).

Setting: University research institute, private IVF center, and molecular testing laboratory.

Patient(s): Twenty five infertile patients in the discovery cohort and 11 patients in the validation cohort.

Interventions(s): None.

Main Outcome Measure(s): A signature set of miRNA associated with the risk of RIF.

Result(s): We designed a reproductive disease-related PanelChip to access endometrium miRNA profiles in patients undergoing IVF. Three major miRNA signatures, including hsa-miR-20b-5p, hsa-miR-155-5p, and hsa-miR-718, were identified using infinite combination signature search algorithm analysis from 25 patients in the discovery cohort undergoing IVF. These miRNAs were used as biomarkers in the validation cohort of 11 patients. Finally, the 3-miRNA signature was capable of predicting patients with RIF with an accuracy >90%.

Conclusion(s): Our findings indicated that specific endometrial miRNAs can be applied as diagnostic biomarkers to predict RIF. Such information will definitely help to increase the success rate of implantation practice.
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http://dx.doi.org/10.1016/j.fertnstert.2021.01.055DOI Listing
April 2021

Breast Cancer Screening Practices and Satisfaction With Healthcare Providers in Chinese Women: A Cross-sectional Study.

Cancer Nurs 2021 Mar 16. Epub 2021 Mar 16.

Author Affiliations: Nursing School, Jilin University (Mss X Zhang, Guo, Wang, Liu, Yang, and Yu, and Dr W Zhang); and the Second Hospital of Jilin University (Dr Li and Ms C Zhang), Changchun, China.

Background: The Chinese government has proposed cancer screening guidelines and launched 2 cancer screening programs to improve screening practices. However, the screening participation rates of Chinese women remain low. Exploring the factors affecting breast cancer screening to promote cancer screening is necessary.

Objective: The aim of this study was to investigate women's satisfaction with healthcare providers and screening practices.

Methods: A cross-sectional survey was conducted from November 2019 to April 2020 in Changchun, Jilin Province, China. The Medical Care Satisfaction Scale and SERVQUAL were used to quantify women's satisfaction with doctors and nurses, respectively.

Results: Women's satisfaction with doctors (68.81 ± 11.08; range, 20-100) and with nurses (87.64 ± 16.17; range, 22-110) was moderate. Compared with women who had not participated in screening within the past 2 years, screened women reported higher satisfaction with doctors (P = .026) and nurses (P = .012). Older age (P = .004) was an independent predictor of women's satisfaction with doctors. Similarly, women's education level (P = .049), chronic disease history (P = .029), and physical examination history (P = .039) were 3 independent predictors of women's satisfaction with nurses.

Conclusions: Future intervention strategies can not only increase the screening rates by providing existing intervention methods but also improve women's satisfaction with healthcare providers to promote screening practices.

Implications For Practice: This article provides a theoretical basis for healthcare providers to increase female screening practices. It also provides references for improving existing intervention methods.
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http://dx.doi.org/10.1097/NCC.0000000000000942DOI Listing
March 2021

Morphological stasis masks ecologically divergent coral species on tropical reefs.

Curr Biol 2021 Mar 29. Epub 2021 Mar 29.

Global Change Institute, The University of Queensland, Brisbane, QLD 4072, Australia; ARC Centre of Excellence for Coral Reef Studies, School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Coral reefs are the epitome of species diversity, yet the number of described scleractinian coral species, the framework-builders of coral reefs, remains moderate by comparison. DNA sequencing studies are rapidly challenging this notion by exposing a wealth of undescribed diversity, but the evolutionary and ecological significance of this diversity remains largely unclear. Here, we present an annotated genome for one of the most ubiquitous corals in the Indo-Pacific (Pachyseris speciosa) and uncover, through a comprehensive genomic and phenotypic assessment, that it comprises morphologically indistinguishable but ecologically divergent lineages. Demographic modeling based on whole-genome resequencing indicated that morphological crypsis (across micro- and macromorphological traits) was due to ancient morphological stasis rather than recent divergence. Although the lineages occur sympatrically across shallow and mesophotic habitats, extensive genotyping using a rapid molecular assay revealed differentiation of their ecological distributions. Leveraging "common garden" conditions facilitated by the overlapping distributions, we assessed physiological and quantitative skeletal traits and demonstrated concurrent phenotypic differentiation. Lastly, spawning observations of genotyped colonies highlighted the potential role of temporal reproductive isolation in the limited admixture, with consistent genomic signatures in genes related to morphogenesis and reproduction. Overall, our findings demonstrate the presence of ecologically and phenotypically divergent coral species without substantial morphological differentiation and provide new leads into the potential mechanisms facilitating such divergence. More broadly, they indicate that our current taxonomic framework for reef-building corals may be scratching the surface of the ecologically relevant diversity on coral reefs, consequently limiting our ability to protect or restore this diversity effectively.
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http://dx.doi.org/10.1016/j.cub.2021.03.028DOI Listing
March 2021

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia.

Arch Virol 2021 Apr 3. Epub 2021 Apr 3.

Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.

A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, in Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of begomovirus infections. The apparently full-length viral component was cloned and sequenced following enrichment of circular DNA by rolling-circle amplification and restriction enzyme digestion. The genome of the virus was 2737 nucleotides in length (KP188831) and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nucleotide sequence similarity (87.7% identity) with ageratum yellow vein virus (AM940137). A satellite molecule was amplified from total DNA by PCR amplification, using the betasatellite-specific primer pair β01/β02. The satellite molecule (1346 nt, KP188832) had structural characteristics like those of other betasatellites associated with begomoviruses and shared the highest nucleotide sequence similarity (84.8% identity) with malvastrum yellow vein betasatellite (MN205547). According to the criteria established for species demarcation for classification of begomoviruses (family Geminiviridae) and betasatellites (family Tolecusatellitidae), respectively, the virus isolate from M. coromandelianum in Cambodia is a previously undescribed novel monopartite begomovirus, for which the name "malvastrum yellow vein Cambodia virus" (MaYVCV) is proposed, and the betasatellite is a previously undescribed novel betasatellite, for which the name "malvastrum yellow vein Cambodia betasatellite" (MaYVKHB) is proposed.
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http://dx.doi.org/10.1007/s00705-021-05016-yDOI Listing
April 2021

Serum Metabolic Profiling Analysis of Chronic Gastritis and Gastric Cancer by Untargeted Metabolomics.

Front Oncol 2021 11;11:636917. Epub 2021 Mar 11.

Departmant of Clinical Laboratory, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang, China.

Purpose: Gastric cancer is a common tumor of the digestive system. Identification of potential molecules associated with gastric cancer progression and validation of potential biomarkers for gastric cancer diagnosis are very important. Thus, the aim of our study was to determine the serum metabolic characteristics of the serum of patients with chronic gastritis (CG) or gastric cancer (GC) and validate candidate biomarkers for disease diagnosis.

Experimental Design: A total of 123 human serum samples from patients with CG or GC were collected for untargeted metabolomic analysis UHPLC-Q-TOF/MS to determine characteristics of the serum. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and heat map were used for multivariate analysis. In addition, commercial databases were used to identify the pathways of metabolites. Differential metabolites were identified based on a heat map with a -test threshold ( < 0.05), fold-change threshold (FC > 1.5 or FC < 2/3) and variable importance in the projection (VIP >1). Then, differential metabolites were analyzed by receiver operating characteristic (ROC) curve to determine candidate biomarkers. All samples were analyzed for fasting lipid profiles.

Results: Analysis of serum metabolomic profiles indicated that most of the altered metabolic pathways in the three groups were associated with lipid metabolism ( < 0.05) and lipids and lipid-like molecules were the predominating metabolites within the top 100 differential metabolites ( < 0.05, FC > 1.5 or FC < 2/3, and VIP >1). Moreover, differential metabolites, including hexadecasphinganine, linoleamide, and N-Hydroxy arachidonoyl amine had high diagnostic performance according to PLS-DA. In addition, fasting lipid profile analysis showed the serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and apolipoprotein A1 (Apo-A1) were decreased concomitant to the progression of the progression of the disease compared with those in the control group ( < 0.05).

Conclusions: Thus, this study demonstrated that lipid metabolism may influence the development of CG to GC. Hexadecasphinganine, linoleamide, and N-Hydroxy arachidonoyl amine were selected as candidate diagnostic markers for CG and GC.
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http://dx.doi.org/10.3389/fonc.2021.636917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991914PMC
March 2021

Inhibition Effect of Zoledronate on the Osteoclast Differentiation of RAW264.7 Induced by Titanium Particles.

Biomed Res Int 2021 4;2021:5578088. Epub 2021 Mar 4.

Department of Orthopaedics, Fuzhou Second Hospital affiliated to Xiamen University, Fujian Province 350007, China.

Objective: This study is aimed at studying the effect of zoledronate (ZOL) on the differentiation of osteoclast precursor RAW264.7 cells induced by titanium (Ti) particles and explores the possibility of preventing and treating periprosthetic osteoporosis using ZOL.

Methods: RAW264.7 cells were cultured in vitro. Ti particles were prepared. The cell proliferation curve of RAW264.7 cells was plotted using the MTT assay to find the best concentration of ZOL for intervention. The cells were divided into three groups: control, Ti particles, and Ti particles+ZOL. The cell morphology was observed using tartaric acid-resistant acid phosphatase (TRAP) staining, and the activity of TRAP in cell supernatant was determined using the biochemical method. The number of bone resorption lacunae was detected using toluidine blue staining. The mRNA expression of , , , and was detected using real-time polymerase chain reaction. The protein expression of RANK, NFATcl, and MMP-9 was detected using Western blot analysis.

Results: Ti particles stimulated the differentiation of RAW264.7 cells into osteoclasts. They also increased the activity of TRAP, number of bone resorption lacunae, and mRNA and protein expression of RANK, NFATcl, and MMP-9. However, ZOL could suppress the effect of TI particles on the osteoclast differentiation of RAW264.7 cells.

Conclusions: ZOL could effectively inhibit the differentiation of RAW264.7 cells into osteoclasts induced by Ti particles, decrease the activity of TRAP, reduce the number of bone resorption lacunae, and decrease the mRNA and protein expression of RANK, NFATcl, and MMP-9. Hence, it may be a promising candidate for preventing and treating periprosthetic osteoporosis after the artificial joint operation.
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http://dx.doi.org/10.1155/2021/5578088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952169PMC
March 2021

Recent Progress in Solar-Induced Direct Biomass-to-Electricity Hybrid Fuel Cell Using Microalgae as Feedstocks.

Front Bioeng Biotechnol 2021 3;9:638971. Epub 2021 Mar 3.

Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou, China.

Microalgae, as potential biodiesel feedstocks, have been widely reported to accumulate oil via genetic engineering techniques, or environmental stress regulation. Recently, the utilization of fuel cell technology to convert biomass into electricity has attracted much more attention due to its high efficiency, low pollution, low noise by microalgae as feedstocks. Normally, platinum and analogous noble metals as catalysts have been already demonstrated although they still exist lots of shortcomings. This mini review presents an overview of various fuel cell technologies with phosphomolybdic acid as catalysts for sustainable energy by using microalgae. Trends from literatures demonstrate that algal-based fuel cells could efficiently generate electricity, and concurrently produce high value-added products. This critical review can provide guiding suggestions for future study of algal-based energy conversion by fuel cell techniques.
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http://dx.doi.org/10.3389/fbioe.2021.638971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982948PMC
March 2021

Gualou Guizhi Granule Protects against OGD/R-Induced Injury by Inhibiting Cell Pyroptosis via the PI3K/Akt Signaling Pathway.

Evid Based Complement Alternat Med 2021 8;2021:6613572. Epub 2021 Mar 8.

Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.

Pyroptosis is a proinflammatory form of regulated cell death that plays an important role in ischemic stroke. Gualou Guizhi granule (GLGZG) is a classic prescription that has been shown to exert neuroprotective effects against cerebral ischemia reperfusion injury. In the present study, we examined the involvement of pyroptosis and its associated mechanism in protecting nerve function. . Primary neurons were exposed to oxygen-glucose deprivation and reperfusion (OGD/R) conditions in the presence or absence of GLGZG. Cellular viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay. The number of apoptoic cells was detected by NeuN and NSE protein expression. The expression levels of the pyroptosis markers, namely, NOD-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1, interleukin-18 (IL-18), and IL-1 were determined by quantitative real-time PCR analysis, western blot, and ELISA analyses as appropriate. Moreover, the expression levels of the PI3K/Akt pathway key proteins were determined by quantitative real-time PCR analysis and western blot assays. To determine the PI3K/Akt pathway involvement in GLGZG-mediated neuroprotection, the PI3K inhibitor LY294002 (LY, 10 M) was added. The expression levels of NeuN, Akt, and p-Akt were evaluated. . It was found that GLGZG could inhibit OGD/R-induced cell apoptosis, increase neuronal cell viability, decrease the production of IL-18 and IL-1, and downregulate the expression levels of pyroptosis markers (NLRP3, ASC, and caspase-1). Furthermore, GLGZG could modulate the PI3K/Akt signaling pathway. Pharmacological inhibition of the PI3K pathway not only abrogated the effects of GLGZG on Akt but also neutralized its prosurvival and antipyroptotic actions. . The findings indicated that GLGZG pretreatment effectively reduced OGD/R-induced injury by inhibiting cell pyroptosis and activating the PI3K/Akt pathway. These data provide important evidence for the therapeutic applications of this regimen in ischemic stroke.
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http://dx.doi.org/10.1155/2021/6613572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960020PMC
March 2021

Deep Learning for Diagnosis and Classification of Obstructive Sleep Apnea: A Nasal Airflow-Based Multi-Resolution Residual Network.

Nat Sci Sleep 2021 12;13:361-373. Epub 2021 Mar 12.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.

Purpose: This study evaluated a novel approach for diagnosis and classification of obstructive sleep apnea (OSA), called Obstructive Sleep Apnea Smart System (OSASS), using residual networks and single-channel nasal pressure airflow signals.

Methods: Data were collected from the sleep center of the First Affiliated Hospital, Sun Yat-sen University, and the Integrative Department of Guangdong Province Traditional Chinese Medical Hospital. We developed a new model called the multi-resolution residual network (Mr-ResNet) based on a residual network to detect nasal pressure airflow signals recorded by polysomnography (PSG) automatically. The performance of the model was assessed by its sensitivity, specificity, accuracy, and F1-score. We built OSASS based on Mr-ResNet to estimate the apnea‒hypopnea index (AHI) and to classify the severity of OSA, and compared the agreement between OSASS output and the registered polysomnographic technologist (RPSGT) score, assessed by two technologists.

Results: In the primary test set, the sensitivity, specificity, accuracy, and F1-score of Mr-ResNet were 90.8%, 90.5%, 91.2%, and 90.5%, respectively. In the independent test set, the Spearman correlation for AHI between OSASS and the RPSGT score determined by two technologists was 0.94 ( < 0.001) and 0.96 ( < 0.001), respectively. Cohen's Kappa scores for classification between OSASS and the two technologists' scores were 0.81 and 0.84, respectively.

Conclusion: Our results indicated that OSASS can automatically diagnose and classify OSA using signals from a single-channel nasal pressure airflow, which is consistent with polysomnographic technologists' findings. Thus, OSASS holds promise for clinical application.
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http://dx.doi.org/10.2147/NSS.S297856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966385PMC
March 2021

Improving the Efficiency of Quantum Dot Sensitized Solar Cells beyond 15% via Secondary Deposition.

J Am Chem Soc 2021 Mar 18;143(12):4790-4800. Epub 2021 Mar 18.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, People's Republic of China.

Low loading is one of the bottlenecks limiting the performance of quantum dot sensitized solar cells (QDSCs). Although previous QD secondary deposition relying on electrostatic interaction can improve QD loading, due to the introduction of new recombination centers, it is not capable of enhancing the photovoltage and fill factor. Herein, without the introduction of new recombination centers, a convenient QD secondary deposition approach is developed by creating new adsorption sites via the formation of a metal oxyhydroxide layer around QD presensitized photoanodes. MgCl solution treated Zn-Cu-In-S-Se (ZCISSe) QD sensitized TiO film electrodes have been chosen as a model device to investigate this secondary deposition approach. The experimental results demonstrate that additional 38% of the QDs are immobilized on the photoanode as a single layer. Due to the increased QD loading and concomitant enhanced light-harvesting capacity and reduced charge recombination, not only photocurrent but also photovoltage and fill factor have been remarkably enhanced. The average PCE of resulted ZCISSe QDSCs is boosted to 15.31% ( = 26.52 mA cm, = 0.802 V, FF = 0.720), from the original 13.54% ( = 24.23 mA cm, = 0.789 V, FF = 0.708). Furthermore, a new certified PCE record of 15.20% has been obtained for liquid-junction QDSCs.
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http://dx.doi.org/10.1021/jacs.1c01214DOI Listing
March 2021

Ring closure strategy leads to potent RIPK3 inhibitors.

Eur J Med Chem 2021 May 9;217:113327. Epub 2021 Mar 9.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, PR China. Electronic address:

Necroptosis is a form of regulated necrotic cell death that is independent of caspases. Receptor-interacting protein kinase 3 (RIPK3) has been identified as a key regulator for necroptosis, and has been proposed as a potential therapeutic target for the treatment of diseases associated with necroptosis. In this report, we describe the design, synthesis, and evaluation of a series of novel RIPK3 inhibitors. The lead compound 38 exhibited potent activity (EC = 0.42 μM) in blocking TNFα, Smac mimetic and z-VAD (TSZ) induced cell death in HT-29 cells. Mechanistic studies showed that compound 38 bound to RIPK3 with high affinity (K = 7.1 nM), and inhibited RIPK3 kinase activity in a ADP-Glo functional assay. In addition, compound 38 displayed good selectivity over another necroptosis regulator RIPK1 (K = 6000 nM). Furthermore, compound 38 demonstrated excellent in vitro safety profiles with minimal inhibition of CYP isozymes and hERG potassium channel. Lastly, compound 38 efficiently blocked hypothermia and death in mice in the TNFα-induced systemic inflammatory response syndrome model.
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http://dx.doi.org/10.1016/j.ejmech.2021.113327DOI Listing
May 2021