Publications by authors named "Yu Liang"

1,077 Publications

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Histone deacetylase inhibitor givinostat attenuates nonalcoholic steatohepatitis and liver fibrosis.

Acta Pharmacol Sin 2021 Aug 2. Epub 2021 Aug 2.

Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200092, China.

Nonalcoholic steatohepatitis (NASH) is a common chronic liver disease that is increasingly prevalent worldwide. Liver inflammation is an important contributor to disease progression from nonalcoholic fatty liver (NAFL) to NASH, but there is a lack of efficient therapies. In the current study we evaluated the therapeutic potential of givinostat, a histone deacetylase (HDAC) inhibitor, in the treatment of NASH in vivo and in vitro. Liver inflammation was induced in mice by feeding a methionine- and choline-deficient diet (MCD) or a fructose, palmitate, cholesterol diet (FPC). The mice were treated with givoinostat (10 mg·kg·d, ip) for 8 or 10 weeks. At the end of the experiment, the livers were harvested for analysis. We showed that givoinostat administration significantly alleviated inflammation and attenuated hepatic fibrosis in MCD-induced NASH mice. RNA-seq analysis of liver tissues form MCD-fed mice revealed that givinostat potently blocked expression of inflammation-related genes and regulated a broad set of lipid metabolism-related genes. In human hepatocellular carcinoma cell line HepG2 and human derived fetal hepatocyte cell line L02, givinostat significantly decreased palmitic acid-induced intracellular lipid accumulation. The benefit of givinostat was further confirmed in FPC-induced NASH mice. Givinostat administration significantly attenuated hepatic steatosis, inflammation as well as liver injury in this mouse model. In conclusion, givinostat is efficacious in reversing diet-induced NASH, and may serve as a therapeutic agent for the treatment of human NASH.
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http://dx.doi.org/10.1038/s41401-021-00725-1DOI Listing
August 2021

Adiponectin Treatment Attenuates Cerebral Ischemia-Reperfusion Injury through HIF-1-Mediated Antioxidation in Mice.

Oxid Med Cell Longev 2021 14;2021:5531048. Epub 2021 Jul 14.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Adiponectin (ADPN) plays an important role in cerebral ischemia-reperfusion injury. Although previous studies have confirmed that ADPN pretreatment has a protective effect on ischemic stroke, the therapeutic effect of ADPN on ischemic stroke and the underlying mechanism are still unclear. In order to clarify these questions, focal transient cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice and ADPN was administered for three times at 6 h, 24 h, and 48 h after reperfusion. Meanwhile, a virus-delivered HIF-1 siRNA was used before ADPN administration. The infarct volume, neurological score, cellular apoptosis, and oxidative stress were assessed at 72 h after reperfusion. The long-term outcome of mice after stroke was recorded as well. The results indicated that ADPN treatment reduced the infarct volume ( = 0.032), neurological deficits ( = 0.047), cellular apoptosis ( = 0.041), and oxidative responses ( = 0.031) at 72 h after MCAO. Moreover, ADPN increased both the protein level and transcriptional activity of HIF-1 as evidenced by the transcription levels of VEGF ( = 0.046) and EPO ( = 0.043) at 72 h after MCAO. However, knockdown of HIF-1 partially reversed the antioxidant and treatment effect of ADPN after cerebral ischemia. In the observation of long-term outcome after ADPN treatment, it demonstrated that ADPN not only prevented the cerebral atrophy ( = 0.031) and the neurological function decline ( = 0.048), but also promoted angiogenesis ( = 0.028) after stroke. In conclusion, our findings suggest that ADPN is effective in treatment of ischemic stroke which could be attributed to the increased antioxidant capacity regulated by HIF-1.
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http://dx.doi.org/10.1155/2021/5531048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298180PMC
July 2021

ADNP prompts the cisplatin-resistance of bladder cancer via TGF-β-mediated epithelial-mesenchymal transition (EMT) pathway.

J Cancer 2021 22;12(17):5114-5124. Epub 2021 Jun 22.

Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, Hunan Normal University, 410081 Changsha, China.

Activity-dependent neuroprotective protein (ADNP) is vital for embryonic development and brain formation. Besides, the upregulated expression of ADNP enhances tumorigenesis in some human tumors like bladder cancer (BC). However, the potential roles of ADNP in drug resistance and the related mechanisms in BC is unknown. We performed this study to elucidate the influence of ADNP in the chemoresistance of BC and tried to explore the underlying molecular mechanism. The expressions of ADNP in BC from progression and non-progression patient specimens were measured by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). experiments including colony formation, cell counting kit-8 (CCK-8), wound healing, and tumorigenesis assay were performed to explore the effects of ADNP on chemoresistance of BC. The impacts of ADNP on TGF-β/Smad signaling pathways were explored by western blot. Our results showed that the expression of ADNP mRNA and protein were significantly upregulated in BC tissues of the patients who suffered tumor-progression via RT-PCR and western blot. Cox regression survival analysis revealed that patients with high ADNP expression closely linked to shorter tumor-free survival. ADNP downregulation in BC showed more sensitive to cisplatin , while ADNP overexpression showed the opposite results. Additionally, we confirmed that ADNP promoted cell migration and EMT, thereby inducing cisplatin resistance, which may be related to TGF-β / Smad signaling pathway.
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http://dx.doi.org/10.7150/jca.58049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317519PMC
June 2021

Self-adaptive metasurface platform based on computer vision.

Opt Lett 2021 Aug;46(15):3520-3523

Programmable metasurfaces allow real-time electromagnetic (EM) manipulation in a digital manner, showing great potential to construct advanced multifunctional EM devices. However, the current programmable metasurfaces typically need human participation to achieve various EM functions. In this Letter, we propose, design, and construct a self-adaptive metasurface platform that can achieve beam control automatically based on image recognition. Such a platform is composed of a metasurface with 36×36 active units, a single-board computer, and two independent cameras that can detect the position of the objects. The single-board computer, Raspberry Pi, is used to calculate the information of the objects and generate the coding sequences to control the digital metasurface based on a low complexity binocular localization algorithm. Such a smart metasurface platform can generate different beams according to the location of the receiver and can be used as intelligent antennas in future communications and radars.
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http://dx.doi.org/10.1364/OL.427527DOI Listing
August 2021

The efficacy and safety of MR-HIFU and US-HIFU in treating uterine fibroids with the volume <300 cm: a meta-analysis.

Int J Hyperthermia 2021 ;38(1):1126-1132

Department of Gynecology, Jiangsu Province Hospital, Nanjing, China.

Background: High-intensity focused ultrasound (HIFU) is a promising and non-invasive therapy for symptomatic uterine fibroids. Currently, the main image-guided methods for HIFU include magnetic resonance-guided (MR-HIFU) and ultrasound-guided (US-HIFU). However, there are few comparative studies on the therapeutic efficacy and safety of MR-HIFU and US-HIFU in treating symptomatic uterine fibroids with a volume <300 cm.

Objective: We performed this meta-analysis to evaluate the efficacy and safety of MR-HIFU and US-HIFU in treating symptomatic uterine fibroids with a volume <300 cm.

Methods: We searched relevant literature in PubMed, EMBASE, Cochrane Library CNKI from inception until 2021. The mean value, the proportion, and their 95% confidence intervals (CIs) were measured by random-effects models. Publication bias was assessed using funnel plots.

Results: 48 studies met our inclusion criteria-28 describing MR-HIFU and 20 describing US-HIFU. The mean non-perfused volume rate (NPVR) was 81.07% in the US-HIFU group and 58.92% in the MR-HIFU group, respectively. The mean volume reduction rates at month-3, month-6, and month-12 were 42.42, 58.72, and 65.55% in the US-HIFU group, while 34.79, 37.39, and 36.44% in the MR-HIFU group. The incidence of post-operative abdominal pain and abnormal vaginal discharge in the US-HIFU group was lower than that of MRI-HIFU. However, post-operative skin burn and sciatic nerve pain were more common in the US-HIFU group compared with MRI-HIFU. The one-year reintervention rate after MR-HIFU was 13.4%, which was higher than 5.2% in the US-HIFU group.

Conclusion: US-HIFU may show better efficiency and safety than MR-HIFU in treating symptomatic fibroids with a volume <300 cm.
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http://dx.doi.org/10.1080/02656736.2021.1954245DOI Listing
January 2021

LncRNA DiGeorge syndrome critical region gene 5: A crucial regulator in malignant tumors.

Biomed Pharmacother 2021 Jul 14;141:111889. Epub 2021 Jul 14.

Department of Hepatopancreatobiliary Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, China. Electronic address:

Long non-coding RNA (lncRNA), a subgroup of ncRNA with a length of more than 200 nt without protein coding function, has been recognized by the academia for its mediating effects of dysregulated expression on the tumorigenesis and development of a variety of tumors. LncRNA DiGeorge syndrome critical region gene 5 (DGCR5), originally found to induce DiGeorge syndrome, has been confirmed to be extremely dysregulated in multiple tumors, which mediates the malignant phenotypes of hepatocellular carcinoma, pancreatic cancer, lung cancer, etc. through the regulation of Wnt/β-catenin, MEK/ERK1/2 and other cancerous signaling pathways as a molecular sponge. Researches on the cancerous derivation-related pathways involved in DGCR5 can provide potential molecular intervention targets for tumor precision treatment. Moreover, liquid biopsy based on the detection of DGCR5 in body fluids is also expected to provide a non-invasive evaluation method for the early diagnosis and prognostic evaluation of malignant tumors.
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http://dx.doi.org/10.1016/j.biopha.2021.111889DOI Listing
July 2021

Tissue Cytokine Adenoid Experssion in Hypertrophic Adenoid Gland in Children with Allergic Rhinitis.

J Coll Physicians Surg Pak 2021 Aug;31(8):903-909

Department of Otolaryngology - Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, P.R. China.

Objective: To investigate the inflammatory pattern in terms of inflammatory cells and cytokines expression in children with adenoid hypertrophy (AH) and coexistent allergic rhinitis (AR).

Study Design: A cross-sectional analytical study.

Place And Duration Of Study: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong P. R. China, from October 2018 to August 2020.

Methodology: A sample of 102 children with AH, who underwent adenoidectomy were enrolled. They were divided into two groups of AH, alone and AH with AR (AH+AR). A routine complete blood count, and the number of eosinophils in adenoid tissue was measured using hematoxylin-eosin staining. The tissue expression of cytokines was carried out using real-time quantitative PCR.

Results: Forty-eight children (47%) were diagnosed with AR. The number and percentage of eosinophils in peripheral blood and adenoid tissue were statistically (p <0.05) higher in the group of AH+AR than AH alone. Furthermore, in patients with AH+AR, the mRNA expression levels of IL-12 and IFN-γ decreased, while IL-4, IL-8, IL-18, IL-33, H2R, LTR1, LTR2 and GCR all increased in adenoid tissue.

Conclusion: The pathological mechanism underlying adenoid hypertrophy in children with comorbid allergic rhinitis can be involved with predominant tissue eosinophilia and type 2 (or Th 2) inflammation. Key Words: Adenoid hypertrophy, Allergic rhinitis, Inflammatory features, Cytokines, Eosinophils.
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http://dx.doi.org/10.29271/jcpsp.2021.08.903DOI Listing
August 2021

Exosomes in hepatocellular carcinoma microenvironment and their potential clinical application value.

Biomed Pharmacother 2021 Jun 26;138:111529. Epub 2021 Mar 26.

Department of Pancreatobiliary Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China. Electronic address:

Hepatocellular carcinoma (HCC) has become a challenging disease in the world today. Due to the limitations on the current diagnosis and treatment as well as its high metastatic ability and high recurrence rate, HCC gradually becomes the second deadliest tumor. Exosomes are one of the types of cell-derived vesicles and can carry intracellular materials such as genetic materials, lipids, and proteins. In recent years, it has been verified that exosomes are linked to numerous physiological and pathological processes, including HCC. However, how exosomes affect HCC progression remains largely unknown. In this review, the exosome-mediated cellular material transfer between cells of different types in the HCC microenvironment and their effects on the behaviors and functions of recipient cells are studied. Furthermore, we also addressed the underlying molecular mechanisms. We believe that new light on the diagnosis of this cancer as well as its treatment strategies will be shed after a collation of literature in this area.
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http://dx.doi.org/10.1016/j.biopha.2021.111529DOI Listing
June 2021

Analysis of factors influencing the network teaching effect of college students in a medical school during the COVID-19 epidemic.

BMC Med Educ 2021 Jul 23;21(1):397. Epub 2021 Jul 23.

Department of Oral Medicine, School of Stomatology, Wannan Medical College, Wuhu, 241002, Anhui, China.

Background: The purpose of this study is to understand the influencing factors of Chinese college students' satisfaction with online teaching and psychological pressure on learning during the novel coronavirus epidemic.

Methods: We assessed the effect of online teaching of 7084 medical students from wannan medical college in March 5 to April 2, 2020 using cluster sampling. The respondents were asked to complete a 7-item self-compiled online teaching satisfaction questionnaire. Chi-square test and multivariate logistic regression analysis are used.

Results: Sex is female (OR = 1.257, 95%CI: 1.132 ~ 1.396), grades are second and third grades (second grades: OR = 1.228, 95%CI: 1.080 ~ 1.397; third grades: OR = 1.197, 95%CI: 1.048 ~ 1.367), normal/unfamiliar learning platform operation (OR = 3.692, 95%CI: 3.321 ~ 4.103) were risk factors for satisfactory teaching effect. In addition, students whose school year system is four-year (OR = 0.870, 95%CI: 0.781 ~ 0.969) and grade 4 and above (OR = 0.594, 95%CI: 0.485 ~ 0.727) were more satisfied with the teaching effect of teachers. And, during the period of the COVID-19 epidemic, the risk factors for college students to have psychological stress were: female (OR = 1.258, 95%CI: 1.096 ~ 1.442), from rural areas (OR = 1.511, 95%CI: 1.312 ~ 1.740), and the academic year system is four-year system (OR = 1.191, 95%CI: 1.028 ~ 1.380), using mobile phones and other learning tools (OR = 1.388, 95%CI: 1.205 ~ 1.600), general/unfamiliar with learning platform operations (OR = 2.273), 95%CI: 1.888 ~ 2.735). While the protective factors for college students' psychological stress included: grade three and four and above (OR = 0.463, 95%CI: 0.387 ~ 0.554; OR = 0.232, 95%CI: 0.187 ~ 0.286), and they think that the teaching effect is satisfactory (OR = 0.314, 95%CI: 0.261 ~ 0.379).

Conclusion: This survey shows that compared with male college students, female college students were more dissatisfied with the teaching effect of teachers and havd greater psychological pressure on learning. Psychological counseling should be strengthened for students in rural areas and those who were not familiar with the operating platform to relieve their psychological pressure on learning.
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http://dx.doi.org/10.1186/s12909-021-02825-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300986PMC
July 2021

Selective Anisotropy of Mechanical Properties in Inconel718 Alloy.

Materials (Basel) 2021 Jul 11;14(14). Epub 2021 Jul 11.

College of Materials and Metallurgy, Guizhou University, Guiyang 550025, China.

Mechanical anisotropy behaviors are investigated in slightly rolled Inconel718 alloy with string-like δ phase and carbides produced during various solid-solution and aging treatments. A weak anisotropy in the strengths and rupture properties at 650 °C is visible, whereas ductility, i.e., reduction in area (RA) and impact toughness (CVN), presents a sound anisotropy behavior. MC carbides promote the operation of slip systems and thus are conducive to weakening the strength anisotropy. The RA anisotropy mainly stems from high-density δ phase particles that provide more crack nucleation sites and stimulate rapid propagation because of the shorter bridge distance between micro-cracks at the rolling direction. In contrast, CVN anisotropy arises from both δ phase and carbides at a lower solid-solution temperature of 940 °C but only depends on carbides at 980 °C where the δ phase fully dissolves. Apart from dislocation motions operated at room temperature, the activated grain boundary processes are responsible for the weak anisotropy of rupture properties at the elevated temperature. This work provides a guideline for technological applications in the hot working processes for Inconel718 alloys.
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http://dx.doi.org/10.3390/ma14143869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307731PMC
July 2021

An analysis of the diagnosis, clinical characteristics, treatment, and survival outcomes of 36 extracranial malignant rhabdoid tumor patients.

Transl Pediatr 2021 Jun;10(6):1598-1609

Department of Pediatric Surgery, Shanghai Childrens' Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Background: Extracranial malignant Rhabdoid tumors (eMRTs) are rare but aggressive lesions in young children. This work aimed to review and analyze the diagnosis, clinical characteristics, treatment, and survival of eMRTs so as to summarize experience for future therapy.

Methods: A total of 36 eMRT cases were treated between January 2008 and August 2019 according to Shanghai Children's Medical Center (SCMC) multimodal protocol of mixed surgery, radiation and chemotherapy involving vincristine, carboplatin, doxorubicin, etoposide, cyclophosphamide and ifosfamide. We collected information including: age at diagnosis, tumor location, disease stage, therapy, outcomes, etc. Overall survival (OS) and event free survival (EFS) were calculated and risk factors for survival were analyzed.

Results: The patients had a median age of 1.80 years at diagnosis (range, 1.4 m-13.42 years), and were followed up for 9.17 months in median (range, 4 d-11.14 y). A total of 16 patients achieved complete remission (CR), and 7 survived without reoccurrence till December 2019. The 3-year EFS was 17.4% (95% CI: 11.0-23.8%) with a 3-year OS of 23.4% (95% CI: 15.8-31.0%). Recurrence was found only in children younger than the median age (1.80 y). Localized staging (Log Rank P=0.039 for OS and P=0.021) and older age (Log Rank P=0.016 for OS and P=0.002 for EFS) were associated with improved outcome. Younger age (Cox regression, OS, OR =2.610, 95% CI: 1.147-5.937, P=0.022; EFS, OR =3.401, 95% CI: 1.495-7.752, P=0.004) were independent risk factors for death and recurrence.

Conclusions: Those eMRTs treated according to SCMC protocol turned out to have poor outcomes. Higher staging at diagnosis and reoccurrence in younger patients remain major threats to the prognosis.
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http://dx.doi.org/10.21037/tp-20-459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261579PMC
June 2021

A Comparative Study of the Effects of Valproate and Oxcarbazepine on Sexual Function, Sperm Quality, and Sex Hormones in Males with Epilepsy.

Biomed Res Int 2021 3;2021:6624101. Epub 2021 Jul 3.

Department of Neurology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Aims: Although several studies have indicated that valproate (VPA) and oxcarbazepine (OXC) cause reproductive endocrine disorders and sexual dysfunction, there remains some controversy regarding these issues in males with epilepsy. This study is aimed at evaluating the effects of VPA and OXC on sexual function, sperm quality, and sex hormones in young males with epilepsy.

Methods: Males with newly diagnosed epilepsy treated with VPA and OXC were recruited, and sexual function questionnaires (International Index of Erectile Function-5 (IIEF-5)), sperm quality, and sex hormone levels were assessed before treatment and at 6 months after treatment with VPA or OXC monotherapy.

Results: Forty-four young males with epilepsy (23 treated with VPA, 21 treated with OXC) and 30 age-matched healthy individuals were recruited for our study. The sexual function, sperm quality, marriage rate, and fertility rate of these young males with epilepsy were lower than those of healthy controls. Sperm quality were significantly reduced in young male patients after 6 months of VPA administration. The level of follicle stimulating hormone (FSH) was increased in patients after OXC treatment. Meanwhile, sexual function and sperm quality were not affected.

Conclusion: Sexual function and sperm quality were reduced in young males with epilepsy. VPA may exert a negative effect on sperm quality, whereas OXC has no harmful effect on sexual function and sperm quality in young males with epilepsy.
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http://dx.doi.org/10.1155/2021/6624101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275390PMC
July 2021

Preparation of 2D supramolecular material doping with TiO for degradation of tetracycline.

Environ Res 2021 Jul 14;202:111689. Epub 2021 Jul 14.

Green Catalysis Center, and College of Chemistry, Zhengzhou University, Henan, 450001, PR China. Electronic address:

Anatase TiO photocatalyst supported on [BMPP][2D-MoO] (BMPP = 1, 3-bis(4-methylpyridine) propane dibromide) was successfully prepared by hydrothermal method. The composites were characterized by field emission scanning electron microscopy (SEM), X-ray diffractometer (XRD), infrared spectroscopy (IR) and energy-dispersive X-ray spectrometer (EDS). The results show that the high content anatase nanoparticles are uniformly loaded on the polyacid supramolecules to form the composite material. The photocatalytic activity of TiO/MoO-BMPP towards the tetracyclines (TCs), a model pollutant, has been investigated. The results show that under a 500W high-pressure xenon lamp, 0.15 g TiO/MoB can degrade 97% of 20 mL (25 mg/L) Tc after 115 min, showing high catalytic activity. In addition, 0.15-TiO/MoB can be easily separated from the reaction system by centrifugation, and the catalyst still maintains high photocatalytic activity after 3 cycles of tests under the same conditions. This shows that it has the potential for recycling. This research provides a new way for the development of new supported catalysts.
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http://dx.doi.org/10.1016/j.envres.2021.111689DOI Listing
July 2021

Plant adaptability in karst regions.

J Plant Res 2021 Jul 13. Epub 2021 Jul 13.

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, College of Life Science, Guangxi Normal University, Ministry of Education, Guilin, China.

Karst ecosystems are formed by dissolution of soluble rocks, usually with conspicuous landscape features, such as sharp peaks, steep slopes and deep valleys. The plants in karst regions develop special adaptability. Here, we reviewed the research progresses on plant adaptability in karst regions, including drought, high temperature and light, high-calcium stresses responses and the strategies of water utilization for plants, soil nutrients impact, human interference and geographical traits on karst plants. Drought, high temperature and light change their physiological and morphological structures to adapt to karst environments. High-calcium and soil nutrients can transfer surplus nutrients to special parts of plants to avoid damage of high nutrient concentration. Therefore, karst plants can make better use of limited water. Human interference also affects geographical distribution of karst plants and their growing environment. All of these aspects may be analyzed to provide guidance and suggestions for related research on plant adaptability mechanisms.
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http://dx.doi.org/10.1007/s10265-021-01330-3DOI Listing
July 2021

Evolution of the interactions between GII.4 noroviruses and histo-blood group antigens: Insights from experimental and computational studies.

PLoS Pathog 2021 Jul 12;17(7):e1009745. Epub 2021 Jul 12.

The Sixth Laboratory, National Vaccine and Serum Institute, Beijing, China.

Norovirus (NoV) is the major pathogen causing the outbreaks of the viral gastroenteritis across the world. Among the various genotypes of NoV, GII.4 is the most predominant over the past decades. GII.4 NoVs interact with the histo-blood group antigens (HBGAs) to invade the host cell, and it is believed that the receptor HBGAs may play important roles in selecting the predominate variants by the nature during the evolution of GII.4 NoVs. However, the evolution-induced changes in the HBGA-binding affinity for the GII.4 NoV variants and the mechanism behind the evolution of the NoV-HBGA interactions remain elusive. In the present work, the virus-like particles (VLPs) of the representative GII.4 NoV stains epidemic in the past decades were expressed by using the Hansenula polymorpha yeast expression platform constructed by our laboratory, and then the enzyme linked immunosorbent assay (ELISA)-based HBGA-binding assays as well as the molecular dynamics (MD) simulations combined with the molecular mechanics/generalized born surface area (MMGBSA) calculations were performed to investigate the interactions between various GII.4 strains and different types of HBGAs. The HBGA-binding assays show that for all the studied types of HBGAs, the evolution of GII.4 NoVs results in the increased NoV-HBGA binding affinities, where the early epidemic strains have the lower binding activity and the newly epidemic strains exhibit relative stronger binding intensity. Based on the MD simulation and MMGBSA calculation results, a physical mechanism that accounts for the increased HBGA-binding affinity was proposed. The evolution-involved residue mutations cause the conformational rearrangements of loop-2 (residues 390-396), which result in the narrowing of the receptor-binding pocket and thus tighten the binding of the receptor HBGAs. Our experimental and computational studies are helpful for better understanding the mechanism behind the evolution-induced increasing of HBGA-binding affinity, which may provide useful information for the drug and vaccine designs against GII.4 NoVs.
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http://dx.doi.org/10.1371/journal.ppat.1009745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297928PMC
July 2021

REVA as A Well-curated Database for Human Expression-modulating Variants.

Genomics Proteomics Bioinformatics 2021 Jul 2. Epub 2021 Jul 2.

Biomedical Pioneering Innovation Center (BIOPIC) & Beijing Advanced Innovation Center for Genomics (ICG), Center for Bioinformatics (CBI) and State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China. Electronic address:

More than 90% of disease- and trait-associated human variants are noncoding. By systematically screening multiple large-scale studies, we compiled REVA, a manually curated database for over 11.8 million experimentally tested noncoding variants with expression-modulating potentials. We provided 2424 functional annotations that could be used to pinpoint the plausible regulatory mechanism of these variants. We further benchmarked multiple state-of-the-art computational tools and found their limited sensitivity remains a serious challenge for effective large-scale analysis. REVA provides high-quality experimentally tested expression-modulating variants with extensive functional annotations, which will be useful for users in the noncoding variants community. REVA is available at http://reva.gao-lab.org.
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http://dx.doi.org/10.1016/j.gpb.2021.06.001DOI Listing
July 2021

Evaluation of vertical transmission of SARS-CoV-2 in utero: Nine pregnant women and their newborns.

Placenta 2021 08 16;111:91-96. Epub 2021 Jun 16.

Hunan Engineering Research Center of Gynecology Disease, Xiangya Hospital, Central South University, Changsha, Hunan Province, 410008, China; Department of Dermatology & Hunan Engineering Research Center of Skin Health and Disease, Xiangya Hospital, Central South University, Changsha, Hunan Province, 410008, China. Electronic address:

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), mainly transmitted by droplets and close contact, has caused a pandemic worldwide as of March 2020. According to the current case reports and cohort studies, the symptoms of pregnant women infected with SARS-CoV-2 were similar to normal adults and may cause a series of adverse consequences of pregnancy (placental abruption, fetal distress, epilepsy during pregnancy, etc.). However, whether SARS-CoV-2 can be transmitted to the fetus through the placental barrier is still a focus of debate.

Methods: In this study, in order to find out whether SARS-CoV-2 can infect fetus through the placental barrier, we performed qualitative detection of virus structural protein (spike protein and nucleoprotein) and targeted receptor protein Angiotensin Converting Enzyme 2 (ACE2), Basigin (CD147) and molecular chaperone GRP78 expression on the placental tissue of seven pregnant women diagnosed with COVID-19 through immunohistochemistry. Amniotic fluid, neonatal throat, anal swab and breastmilk samples were collected immediately in the operating room or delivery room for verification after delivery, which were all tested for SARS-CoV-2 by reverse transcriptionpolymerase chain reaction (RT-PCR).

Results/discussion: The result showed that CD147 was expressed on the basal side of the chorionic trophoblast cell membrane and ACE2 was expressed on the maternal side, while GRP78 was strongly expressed in the cell membrane and cytoplasm. The RT-PCR results of Amniotic fluid, neonatal throat, anal swab and breastmilk samples were all negative. On the basis of these findings, we speculated that it may be due to the placental barrier between mother and baby, for example, villous matrix and interstitial blood vessels have low expression of virus-related receptors (ACE2, CD147, GRP78), the probability of vertical transmission of SARS-CoV-2 through the placenta is low.
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http://dx.doi.org/10.1016/j.placenta.2021.06.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245148PMC
August 2021

Structural, spectroscopic and DFT theoretical studies of phosphorescent CuIPS-containing cuprous complexes.

Acta Crystallogr C Struct Chem 2021 Jul 7;77(Pt 7):331-339. Epub 2021 Jun 7.

College of Materials and Chemistry, China Jiliang University, Hangzhou 310018, People's Republic of China.

Luminescent cuprous complexes are important coordination compounds due to their relative abundance, low cost and ability to display excellent luminescence. The structures of two CuIPS-type cuprous complexes, namely, iodido(thiourea-κS)bis(triphenylphosphane-κP)copper(I), [CuI(CHNS)(CHP)] or [CuI(TU)(TPP)] (I), and (2,3-dihydrobenzimidazole-2-thione-κS)iodidobis(triphenylphosphane-κP)copper(I), [CuI(CHNS)(CHP)] or [CuI(DHBIT)(TPP)] (II), are described. In these two structures, the complex molecules of both are constructed by one copper(I) centre, one iodide ion, two TPP ligands and one thione ligand (TU for I and DHBIT for II). The copper(I) centres of I and II are both located in a distorted CuIPS tetrahedron and are coordinated by two P atoms from two TPP ligands, one S atom from the thione ligand and the I atom. The UV-Vis absorption and photoluminescence properties of these CuIPS-type cuprous complexes have been studied using crystalline powder samples. Detailed time-dependent density functional theory (TD-DFT) calculations and wavefunction analysis reveal that the pale-blue-green phosphorescence emission should originate from intra-ligand (TPP for I and DHBIT for II) charge transfer, with a small component of the metal-to-ligand charge transfer (IL+ML)CT excited state.
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http://dx.doi.org/10.1107/S2053229621005696DOI Listing
July 2021

Theoretical and Experimental Study of 3-Pentanol Autoignition: Ab Initio Calculation, Shock Tube Experiments, and Kinetic Modeling.

J Phys Chem A 2021 Jul 2;125(27):5976-5989. Epub 2021 Jul 2.

Key Laboratory for Power Machinery and Engineering of M. O. E, Shanghai Jiao Tong University, 200240 Shanghai, P. R. China.

3-Pentanol is a potential alternative fuel or a green fuel additive for modern engines. The H-abstraction reactions from 3-pentanol by H, CH, HO, and OH radicals are significant in the 3-pentanol oxidation process. However, corresponding rate constants are forced to rely on either analogy from -butanol or estimation from alkanes due to a lack of direct experimental and theoretical study. In this work, stationary points on the potential energy surfaces (PESs) were calculated with the high-level DLPNO-CCSD(T)/CBS(T-Q)//M06-2X/cc-pVTZ method, which is further used to benchmark against the CBS-QB3 method. Then, the high-pressure limit rate constants for target reactions, over a broad range of temperature (400-2000 K), were calculated with the phase-space theory and conventional transition state theory. A comparison was made between the calculated rate constants and the values available in Carbonnier et al. [ 2019, 37(1), 477-484]. The rate constants for the above H-abstraction reactions in the Carbonnier model were updated with the calculated results, followed by a modification based on the computed results of 3-pentanol + HO to obtain the revised model. Validation against the shock tube (ST) and the jet-stirred reactor (JSR) measurements from the literature proved the revised model an optimal one. Furthermore, using an ST, ignition delay times (IDTs) for the 3-pentanol/air mixtures were measured spanning a temperature range of 920-1450 K, pressures of 6, 10, and 20 bar, and equivalence ratios of 0.5, 1.0, and 1.5. Generally, IDTs decrease with increasing temperature and reflected shock pressure. Improved predictions to present experimental data were obtained by using the revised model as compared with the Carbonnier model. Finally, sensitivity analysis was conducted using the revised model to gain an in-depth comprehension of the 3-pentanol autoignition.
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http://dx.doi.org/10.1021/acs.jpca.1c02713DOI Listing
July 2021

[Capillary electrophoresis-mass spectrometry and its application to proteomic analysis].

Se Pu 2020 Oct;38(10):1117-1124

Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Proteomic analysis plays an important role in basic biological studies and precision medicine. However, real samples contain numerous proteins with a wide dynamic distribution range. Such high complexity of the samples has a drastic effect on the identification coverage of proteins. Consequently, with advancements in mass spectrometry (MS) technology, concomitant improvements in separation technologies for simplifying the sample should be critical. With the advantages of small sample loading volume, high separation efficiency, and high speed, capillary electrophoresis (CE) coupled to MS has been gained much attention in the field of proteomics research. A nanoflow sheath liquid interface and a sheathless interface have been developed and commercialized, boosting the development of the CE-MS technology. Capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), and capillary electrochromatography (CEC) have been successfully combined with MS, and CZE-MS has widespread application. In proteomic research, the "bottom-up" strategy, which is based on the separation and identification of enzymatic peptides, is widely applied. With the limit of detection as low as 1 zmol for peptides, CE-MS has been successfully applied to single-cell proteomic analysis. Besides, CE is complementary to reversed-phase liquid chromatography (RPLC), providing a new approach for the separation and identification of peptides with similar hydrophobic properties (especially, post-translational peptides). The "top-down" strategy, which is based on the separation and identification of intact proteins, can directly provide more accurate and complete information about proteins. For protein separation, CE is advantageous in terms of the high separation resolution and high protein recovery, thereby improving the sensitivity and coverage of protein identification. Native MS enables successful identification and characterization of protein complexes under nondenaturing conditions. Because of the good compatibility of CE with MS, attempts have been made to use CE coupled with native MS for the separation and identification of protein complexes. In this review, the development of the CE-MS technology is first reported, including a robust and sensitive CE-MS interface, and a separation mode coupled to MS. Then, the application of the CE-MS technology to "bottom-up", "top-down" and native MS analysis is discussed. The superiority of CE-MS in proteomic analysis is also emphasized. Finally, the promising future prospects of CE-MS are discussed.
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http://dx.doi.org/10.3724/SP.J.1123.2020.03005DOI Listing
October 2020

Deformation Behavior and Tensile Properties of the Semi-Equiaxed Microstructure in Near Alpha Titanium Alloy.

Materials (Basel) 2021 Jun 18;14(12). Epub 2021 Jun 18.

College of Materials and Metallurgy, Guizhou University, Guiyang 550025, China.

The tensile deformation and fracture behavior of a particular semi-equiaxed microstructure (S-EM) in a near alpha titanium alloy TA19 are investigated by an in situ method. In the S-EM, the thin β lamellae grow through the equiaxed α phase (α), and the original α/β interface in the bimodal microstructure largely disappears, forming a blurry interface between the semi-equiaxed α phase (equiaxed α phase that is grew through by the thin β lamellae) and the transformed β microstructure (β). The formation of dense slip bands inside the semi-equiaxed α phase in the S-EM is inhibited by the thin β lamellae during the tensile deformation. The special characteristics of the S-EM reduce the stress concentration at the interface, and the crack initiation probability in the blurry semi-α/β interface decreased compared to the distinct α/β interface in a conventional equiaxed microstructure (EM). Moreover, the ultimate tensile strength of the S-EM is higher than that of the EM with a slight loss of plasticity.
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http://dx.doi.org/10.3390/ma14123380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234885PMC
June 2021

Sociodemographic factors associated with the first administration of anti-seizure medication in patients with focal epilepsy in Western China.

BMC Neurol 2021 Jun 29;21(1):251. Epub 2021 Jun 29.

Department of Neurology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.

Background: Epilepsy is a severe chronic neurologic disease with a prevalence of 0.7% worldwide; anti-seizure medications (ASMs) are the mainstay of epilepsy treatment. The effects of sociodemographic factors on the characteristics of initial treatment in patients with newly diagnosed focal epilepsy in Western China are unknown. This study was conducted to explore sociodemographic factors associated with initial treatment characteristics.

Methods: Patients with focal epilepsy on continuous ASM treatment who visited to our epilepsy center at Sichuan Provincial People's Hospital between January 2018 and December 2019 were recruited. Data on initial treatment status and sociodemographic variables were obtained from the patients with a questionnaire designed by our researchers. We examined whether sociodemographic factors were associated with epileptic patients' access to neurologists and prescriptions of individual ASMs.

Results: A total of 569 patients completed this study. We found that patients with a higher education level, aged < 16 years, and with a higher household disposable income were more likely to receive treatment from a neurologist than their counterparts. Patients with a lower personal income level and who were treated at a junior hospital were more likely to receive prescriptions for carbamazepine, and those who were younger than 16 years were less likely to receive prescriptions for carbamazepine and oxcarbazepine. Patients with a higher education level, with a higher household disposable income level, who were younger than 16 years, and who were treated at a senior hospital were more likely to receive prescriptions for levetiracetam than their counterparts. Adult, female patients with focal epilepsy treated at a senior hospital were more likely to receive prescriptions for lamotrigine.

Conclusions: This observation suggests that sociodemographic characteristics are associated with access to neurologists and prescriptions of individual antiepileptic drugs. These data may help public health officials establish guidelines for doctors and distribute resources according to the needs of different patient groups.
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http://dx.doi.org/10.1186/s12883-021-02282-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240397PMC
June 2021

A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens.

BMC Microbiol 2021 Jun 28;21(1):197. Epub 2021 Jun 28.

College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China.

Background: Microfluidic chip detection technology is considered a potent tool for many bioanalytic applications. Rapid detection of foodborne pathogens in the early stages is imperative to prevent the outbreak of foodborne diseases, known as a severe threat to human health. Conventional bacterial culture methods for detecting foodborne pathogens are time-consuming, laborious, and lacking in pathogen diagnosis. To overcome this problem, we have created an embedded paper-based microchip based on isothermal loop amplification (LAMP), which can rapidly and sensitively detect foodborne pathogens.

Results: We embed paper impregnated with LAMP reagent and specific primers in multiple reaction chambers of the microchip. The solution containing the target pathogen was injected into the center chamber and uniformly distributed into the reaction chamber by centrifugal force. The purified DNA of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, and Vibrio parahaemolyticus has been successfully amplified and directly detected on the microchip. The E. coli O157:H7 DNA was identified as low as 0.0134 ng μL. Besides, the potential of this microchip in point-of-care testing was further tested by combining the on-chip sample purification module and using milk spiked with Salmonella spp.. The pyrolyzed milk sample was filtered through a polydopamine-coated paper embedded in the inside of the sample chamber. It was transported to the reaction chamber by centrifugal force for LAMP amplification. Then direct chip detection was performed in the reaction chamber embedded with calcein-soaked paper. The detection limit of Salmonella spp. in the sample measured by the microchip was approximately 12 CFU mL.

Conclusion: The paper embedded LAMP microchip offers inexpensive, user-friendly, and highly selective pathogen detection capabilities. It is expected to have great potential as a quick, efficient, and cost-effective solution for future foodborne pathogen detection.
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http://dx.doi.org/10.1186/s12866-021-02223-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240391PMC
June 2021

Upgrading Octane Number of Naphtha by a Robust and Easily Attainable Metal-Organic Framework through Selective Molecular Sieving of Alkane Isomers.

Chemistry 2021 Jun 26. Epub 2021 Jun 26.

Hoffmann Institute of Advanced Materials, Shenzhen Polytechnic, 7098 Liuxian Blvd., Nanshan District, Shenzhen, Guangdong, 518055, P. R. China.

The separation of alkanes, particularly monobranched and dibranched isomers, is of paramount importance in the petrochemical industry for optimizing the feedstock of ethylene production as well as for upgrading the octane number of gasoline. Here, we report the full separation of linear/monobranched alkanes from their dibranched isomers by a robust and easily scalable metal-organic framework material, Co (HCOO) . The compound completely excludes dibranched alkanes but adsorbs their linear and monobranched isomers, as evidenced by single-component and multicomponent adsorption measurements. More importantly, the material exhibits excellent performance in separating naphtha and is capable of providing high quality feedstock for the production of ethylene and gasoline components with high octane number, making it a promising candidate for naphtha separation in petrochemical industry.
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http://dx.doi.org/10.1002/chem.202101871DOI Listing
June 2021

Immunogenicity and safety levels of inactivated quadrivalent influenza vaccine in healthy adults via meta-analysis.

Hum Vaccin Immunother 2021 Jun 22:1-10. Epub 2021 Jun 22.

The Fifth Department of Biological Products, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China.

: The aim of the current study was to evaluate immunogenicity and safety levels of human inactivated quadrivalent influenza vaccine (QIV) which includes two A strains (A/H1N1, A/H3N2) and two B lineages (B/Victoria, B/Yamagata) in healthy adults via meta-analysis. : Searches were conducted in PubMed, Cochrane Library, ClinicalTrials.gov, and EMBASE databases published in 2011-2020 according to inclusion and exclusion criteria. The purpose was to collect and perform meta-analysis of related randomized clinical trial (RCT) data concerning safety and immunogenicity levels of human QIV compared with inactivated trivalent influenza vaccine (TIV). : A total of 9 literatures were included. There was no significant difference in the seroconversion(SCR) and seroprotection(SPR) between QIV and TIV for influenza A strains (A/H1N1, A/H3N2) and the B lineage included in the TIV. QIV showed superior efficacy for the B lineage not included in the TIV: SCR RR of 2.20 (95%CI: 1.44-3.37, = .0003) and SPR RR of 1.34 (95%CI: 1.10-1.63, = .004) for B/Victoria, and SCR RR of 1.88 (95%CI: 1.53-2.31, < .00001) and SPR RR of 1.11 (95%CI: 1.03-1.19, = .006) for B/Yamagata, respectively. There were no significant differences between QIV and TIV for local and systemic adverse events(AE) post-vaccination. : In adults 18-64 years old, QIV not only produced similar immunogenicity and safety levels to TIV, but also had better immunogenicity against influenza B vaccine strains not included in TIV.
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http://dx.doi.org/10.1080/21645515.2021.1932218DOI Listing
June 2021

Outcome of unstable pertrochanteric fractures in high-risk geriatric treated with external fixators.

Eur J Orthop Surg Traumatol 2021 Jun 21. Epub 2021 Jun 21.

Department of Orthopaedics, Panzhihua Municipal Central Hospital, Panzhihua, No. 34 on the Street of Yi kang, Panzhihua City, Province Sichuan, China.

Purpose: To review the outcome of high-risk geriatrics with unstable pertrochanteric fractures treated with external fixator.

Methods: Eighteen consecutive patients with pertrochanteric fractures were operated with external fixator by using closed reduction technique. AO type, accompanying diseases, operating time, transfused blood units, duration of hospitalization, complications, healing time and mortality were recorded. Patients were followed clinically and radiologically for two years at least. The Harris Hip Score was used to document hip function at each regular follow-up.

Results: Fifteen patients with average age of 71.5 years were available for final evaluation. The mean operating time was 41.1 min and union was achieved in all cases with an average time of 16 weeks. The mean Harris Hip Score at one, three, six, twelve and twenty-four months post-operatively was 42.07 ± 8.55; 55.07 ± 11.62; 70.07 ± 10.32; 86.27 ± 9.06 and 89.27 ± 8.81 respectively. Complications included all the patients undergoing loss of some motion in the knee and four of them had to experience revision surgery because of knee stiffness, ten cases of pin-tract infections, seven cases of deep venous thrombosis, two cases of migration of the screws and two cases limb shortening.

Conclusions: The use of external fixator in high-risk geriatric provided a satisfactory long-term efficacy, but the short-term functional results were quite unsatisfactory owing to high complication rate. Therefore, the advantages and disadvantages should be fully weighed when using external fixators, which was especially suitable for those patients who could not tolerate prolonged operative time and open surgery.

Level Of Evidence Iv: Therapeutic study, level 4.
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http://dx.doi.org/10.1007/s00590-021-03066-1DOI Listing
June 2021

Identification of Methylation-Regulated Differentially Expressed Genes and Related Pathways in Hepatocellular Carcinoma: A Study Based on TCGA Database and Bioinformatics Analysis.

Front Oncol 2021 3;11:636093. Epub 2021 Jun 3.

Department of Internal Oncology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.

Background: In recent years, DNA methylation modification has been shown to be a critical mechanism in the field of epigenetics.

Methods: Hepatocellular carcinoma (HCC) data were obtained from The Cancer Genome Atlas project, including RNA expression profiles, Illumina Human Methylation 450K BeadChip data, clinical information, and pathological features. Then, differentially expressed genes (DEGs) and differentially methylated genes were identified using R software. Methylation-regulated DEGs (MeDEGs) were further analyzed using Spearman's correlation analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID database and ClueGO in Cytoscape software. Kaplan-Meier survival analysis explored the relationship between methylation, expression of MeDEGs, and survival time. Gene set enrichment analysis (GSEA) was conducted to predict the function of prognosis-related MeDEGs.

Results: A total of nine up-regulated and 72 down-regulated MeDEGs were identified. GO and KEGG pathway analyses results indicated that multiple cancer-related terms were enriched. Kaplan-Meier survival analysis showed that the methylation status of four MeDEGs (CTF1, FZD8, PDK4, and ZNF334) was negatively associated with overall survival. Moreover, the methylation status of CDF1 and PDK4 was identified as an independent prognostic factor. According to GSEA, hypermethylation of prognosis-related MeDEGs was enriched in pathways that included "Spliceosome", "Cell cycle", "RNA degradation", "RNA polymerase", "DNA replication", "Mismatch repair", "Base excision repair", "Nucleotide excision repair", "Homologous recombination", "Protein export", and "Pyrimidine metabolism".

Conclusions: Aberrant DNA methylation plays a critical role in malignant progression of HCC. Prognosis-related MeDEGs identified in this research may be potential biomarkers and targets in diagnosis and treatment.
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http://dx.doi.org/10.3389/fonc.2021.636093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209385PMC
June 2021

EPSOL: sequence-based protein solubility prediction using multidimensional embedding.

Authors:
Xiang Wu Liang Yu

Bioinformatics 2021 Jun 18. Epub 2021 Jun 18.

School of Computer Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China.

Motivation: The heterologous expression of recombinant protein requires host cells, such as Escherichia coli, and the solubility of protein greatly affects the protein yield. A novel and highly accurate solubility predictor that concurrently improves the production yield and minimizes production cost, and that forecasts protein solubility in an E. coli expression system before the actual experimental work is highly sought.

Results: In this paper, EPSOL, a novel deep learning architecture for the prediction of protein solubility in an E. coli expression system, which automatically obtains comprehensive protein feature representations using multidimensional embedding, is presented. EPSOL outperformed all existing sequence-based solubility predictors and achieved 0.79 in accuracy and 0.58 in Matthew's correlation coefficient. The higher performance of EPSOL permits large-scale screening for sequence variants with enhanced manufacturability and predicts the solubility of new recombinant proteins in an E. coli expression system with greater reliability.

Availability And Implementation: EPSOL's best model and results can be downloaded from GitHub (https://github.com/LiangYu-Xidian/EPSOL).

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab463DOI Listing
June 2021

Ultrathin Trimetal-Organic Framework Nanosheet Electrocatalysts for the Highly Efficient Oxygen Evolution Reaction.

ACS Omega 2021 Jun 27;6(22):13946-13952. Epub 2021 May 27.

State Key Laboratory of Chem/Bio-Sensing and Chemometrics, Provincial Hunan Key Laboratory for Graphene Materials and Devices, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.

Synthesis of ultrathin metal-organic framework (MOF) nanosheets for highly efficient oxygen evolution reaction (OER) is prevalent, but still many challenges remain. Herein, a facile and efficient three-layer method is reported for the synthesis of NiCoFe-based trimetallic MOF nanosheets, which can be directly used for the oxygen evolution reaction in alkaline conditions. The physical characterization and morphology of trimetallic MOF nanosheets were characterized by powder X-ray diffraction (PXRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). By optimizing the molar ratio of Ni/Co/Fe atoms, a series of MOFs with different metal proportions were synthesized. Among them, the as-prepared (NiCo)Fe-MOF nanosheets can deliver a current density of 10 mA cm at a low overpotential of 245 mV with a small Tafel slope of 50.9 mV dec in an alkaline electrolyte and exhibit excellent stability. More importantly, through the characterization of the intermediates in the OER process, the possible source of the catalytic active species is the electrochemically transformed metal hydroxides and oxyhydroxides.
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http://dx.doi.org/10.1021/acsomega.0c01853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190793PMC
June 2021

Downhill Running Decreases the Acetylation of Tubulins and Impairs Autophagosome Degradation in Rat Skeletal Muscle.

Med Sci Sports Exerc 2021 Jun 11. Epub 2021 Jun 11.

School of Sports Science, Beijing Sport University, Beijing, CHINA College of Rehabilitation, Gannan Medical University, Ganzhou, CHINA.

Purpose: This study was designed to probe the effect of downhill running on microtubule acetylation and autophagic flux in rat skeletal muscle.

Methods: Sprague-Dawley rats were subjected to an exercise protocol of a 90-min downhill run with a slope of -16° and a speed of 16 m/min, and then the soleus was sampled at 0 h, 12 h, 24 h, 48 h, and 72 h after exercise. Protein expression levels of microtubule-associated protein 1 light chain 3 (LC3), p62/sequestosome 1 (p62), α-tubulin and acetylated α-tubulin (AcK40 α-tubulin) were detected by Western blotting. Alpha-tubulin was costained with acetylated α-tubulin (AcK40 α-tubulin) or cytoplasmic dynein intermediate chain in a single muscle fiber, and LC3 was costained with lysosomal associated membrane protein 1 (LAMP1) in cryosections. To assess autophagic flux in vivo, colchicine or vehicle was injected intraperitoneally 3 days before the exercise experiment, and the protein levels of LC3 and p62 were measured by Western blotting.

Results: Downhill running induced a significant increase in the protein levels of LC3-II and p62, while the level and proportion of AcK40 α-tubulin were markedly decreased. Furthermore, the amount of dynein on α-tubulin was decreased after downhill running, and autophagosomes accumulated in the middle of myofibrils. Importantly, LC3-II flux was decreased following downhill running compared with that in the control group.

Conclusions: A bout of downhill running decreases microtubule acetylation, which may impair dynein recruitment and autophagosome transportation, causing blocked autophagic flux.
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http://dx.doi.org/10.1249/MSS.0000000000002728DOI Listing
June 2021
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