Publications by authors named "Yu Li"

5,422 Publications

  • Page 1 of 1

Investigating distortions in perceptual stability during different self-movements using virtual reality.

Perception 2022 Aug 9:3010066221116480. Epub 2022 Aug 9.

Virtual Reality Research (VR2) Facility, Division of Neuroscience and Experimental Psychology, 12203University of Manchester, Manchester, UK.

Using immersive virtual reality (the HTC Vive Head Mounted Display), we measured both bias and sensitivity when making judgements about the scene stability of a target object during both active (self-propelled) and passive (experimenter-propelled) observer movements. This was repeated in the same group of 16 participants for three different observer-target movement conditions in which the instability of a target was yoked to the movement of the observer. We found that in all movement conditions that the target needed to move with (in the same direction) as the participant to be perceived as scene-stable. Consistent with the presence of additional available information (efference copy) about self-movement during active conditions, biases were smaller and sensitivities to instability were higher in these relative to passive conditions. However, the presence of efference copy was clearly not sufficient to completely eliminate the bias and we suggest that the presence of additional visual information about self-movement is also critical. We found some (albeit limited) evidence for correlation between appropriate metrics across different movement conditions. These results extend previous findings, providing evidence for consistency of biases across different movement types, suggestive of common processing underpinning perceptual stability judgements.
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http://dx.doi.org/10.1177/03010066221116480DOI Listing
August 2022

Molecular insights into the catalytic oxidation of methanol-to-olefins wastewater with phosphoric acid modified sludge biochar.

Chemosphere 2022 Aug 6;307(Pt 2):135938. Epub 2022 Aug 6.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address:

With the development of methanol-to-olefin (MTO) process, the effective disposal of wastewater was one key factor for the long-period and benign development of this technology. Herein, a sludge-based biochar catalyst (GSC-P) was synthesized and used in photo-Fenton reaction for the degradation of MTO wastewater from the outlet of a biological aerated filter. More iron was distributed on the surface of GSC-P catalyst, facilitating the photo-Fenton oxidation of MTO wastewater, with chemical oxygen demand (COD) removal rate of 75.4% and total organic carbon (TOC) removal rate of 62.5%. The 2223 unique molecular formulas assigned by a Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in the original MTO wastewater showed that CHO compounds shared the lowest peak numbers (20.2%) but the highest peak abundance (51.6%) among the four groups. Besides, lipids, unsaturated hydrocarbons, lignins and proteins were the main structural types. After photo-Fenton treatment of 60 min, there were 56.7%-74.0% of compounds removed by the analysis of van Krevelen diagram, indicating that the MTO wastewater was degraded efficiently. Three possible evolution processes of dissolved organic matters during the photo-Fenton reaction were disclosed at the molecular-level.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135938DOI Listing
August 2022

Identification of the 12-oxo-phytoeienoic acid reductase (OPR) gene family in pepper (Capsicum annuum L.) and functional characterization of CaOPR6 in pepper fruit development and stress response.

Genome 2022 Aug 9. Epub 2022 Aug 9.

Jiangxi Agricultural University, Nanchang, Jiangxi, China;

The 12-oxo-phytoeienoic acid reductase (OPR) is a kind of enzyme in octadecanoid biosynthesis pathway, which determines the biosynthesis of jasmonic acid. Although the roles of OPRs have been extensively studied in several crop plants, little is known about the biological functions of OPR encoding genes in Capsicum annuum plants. In this study, seven OPR family genes (CaOPR1-7) were identified from the C. annuum genome. The physical and chemical properties of CaOPR1-7 were further analyzed, including gene expression patterns, promoter elements and chromosomal locations. The results showed that the seven CaOPR homologous could be divided into two subgroups, and CaOPR6 was highly similar to AtOPR3 in Arabidopsis. The expression of CaOPR6 was significantly induced by various stresses such as cold, salt and pathogen infection, indicating that CaOPR6 plays important roles in response to abiotic and biotic stresses. Overall, these findings improve the understanding of the biological functions of CaOPR6 in the development of pepper fruit and stress response of pepper plants, and facilitate further studies on the molecular biology of OPR proteins in Solanaceae vegetables.
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http://dx.doi.org/10.1139/gen-2022-0037DOI Listing
August 2022

Effect of a Peer-Led Behavioral Intervention for Emergency Department Patients at High Risk of Fatal Opioid Overdose: A Randomized Clinical Trial.

JAMA Netw Open 2022 Aug 1;5(8):e2225582. Epub 2022 Aug 1.

Brown University School of Public Health, Providence, Rhode Island.

Importance: Fatal and nonfatal opioid overdoses are at record levels, and emergency department (ED) visits may be an opportune time to intervene. Peer-led models of care are increasingly common; however, little is known about their effectiveness.

Objective: To evaluate the effect of a peer-led behavioral intervention compared with the standard behavioral intervention delivered in the ED on engagement in substance use disorder (SUD) treatment within 30 days after the ED encounter.

Design, Setting, And Participants: This randomized clinical trial recruited 648 patients from 2 EDs from November 15, 2018, to May 31, 2021. Patients were eligible to participate if they were in the ED for an opioid overdose, receiving treatment related to an opioid use disorder, or identified as having had a recent opioid overdose.

Interventions: Participants were randomly assigned to receive a behavioral intervention from a certified peer recovery specialist (n = 323) or a standard intervention delivered by a hospital-employed licensed clinical social worker (n = 325). A certified peer recovery specialist was someone with at least 2 years of recovery who completed a 45-hour training program and had 500 hours of supervised work experience. After the ED intervention, the certified peer recovery specialists offered continued contact with participants for up to 90 days.

Main Outcomes And Measures: The primary outcome was receipt of SUD treatment within 30 days of enrollment, assessed with deterministic linkage of statewide administrative databases. Treatment engagement was defined as admission to a formal, publicly licensed SUD treatment program or receipt of office-based medication for opioid use disorder within 30 days of the initial ED visit.

Results: Among the 648 participants, the mean (SD) age was 36.9 (10.8) years, and most were male (442 [68.2%]) and White (444 [68.5%]). Receipt of SUD treatment occurred for 103 of 323 participants (32%) in the intervention group vs 98 of 325 participants (30%) in the usual care group within 30 days of the ED visit. Among all participants, the most accessed treatments were outpatient medication for opioid use disorder (buprenorphine, 119 [18.4%]; methadone, 44 [6.8%]) and residential treatment (44 [6.8%]).

Conclusions And Relevance: Overall, this study found that a substantial proportion of participants in both groups engaged in SUD treatment within 30 days of the ED visit. An ED-based behavioral intervention is likely effective in promoting treatment engagement, but who delivers the intervention may be less influential on short-term outcomes. Further study is required to determine the effects on longer-term engagement in SUD care and other health outcomes (eg, recurrent overdose).

Trial Registration: ClinicalTrials.gov Identifier: NCT03684681.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.25582DOI Listing
August 2022

Progress on aptamer-based SERS sensors for food safety and quality assessment: methodology, current applications and future trends.

Crit Rev Food Sci Nutr 2022 Aug 9:1-18. Epub 2022 Aug 9.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People's Republic of China.

It is well known that food safety has aroused extensive attentions from governments to researchers and to food industries. As a versatile technology based on molecular interactions, aptamer sensors which could specifically identify a wide range of food contaminants have been extensively studied in recent years. Surface-enhanced Raman spectroscopy integrated aptamer combines the advantages of both technologies, not only in the ability to specifically identify a wide range of food contaminants, but also in the ultra-high sensitivity, simplicity, portable and speed. To provide beneficial insights into the evaluation techniques in the field of food safety, we offer a comprehensive review on the design strategies for aptamer-SERS sensors in different scenarios, including non-nucleic acid amplification methods ("on/off" mode, sandwich mode, competition model and catalytic model) and nucleic acid amplification methods (hybridization chain reaction, rolling circle amplification, catalytic hairpin assembly). Meanwhile, a special attention is paid to the application of aptamer-SERS sensors in biological (foodborne pathogenic, bacteria and mycotoxins) and chemical contamination (drug residues, metal ions, and food additives) of food matrix. Finally, the challenges and prospects of developing reliable aptamer-SERS sensors for food safety were discussed, which are expected to offer a strong guidance for further development and extended applications.
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http://dx.doi.org/10.1080/10408398.2022.2108370DOI Listing
August 2022

Host Plant Selection Imprints Structure and Assembly of Fungal Community along the Soil-Root Continuum.

mSystems 2022 Aug 9:e0036122. Epub 2022 Aug 9.

College of Biology and the Environment, Nanjing Forestry University, Nanjing, China.

The soil fungal community plays pivotal roles in soil nutrient cycling and plant health and productivity in agricultural ecosystems. However, the differential adaptability of soil fungi to different microenvironments (niches) is a bottleneck limiting their application in agriculture. Hence, the understanding of ecological processes that drive fungal microbiome assembly along the soil-root continuum is fundamental to harnessing the plant-associated microbiome for sustainable agriculture. Here, we investigated the factors that shape fungal community structure and assembly in three compartment niches (the bulk soil, rhizosphere, and rhizoplane) associated with tobacco (Nicotiana tabacum L.), with four soil types tested under controlled greenhouse conditions. Our results demonstrate that fungal community assembly along the soil-root continuum is governed by host plant rather than soil type and that soil chemical properties exert a negligible effect on the fungal community assembly in the rhizoplane. Fungal diversity and network complexity decreased in the order bulk soil > rhizosphere > rhizoplane, with a dramatic decrease in Ascomycota species number and abundance along the soil-root continuum. However, facilitations (positive interactions) were enhanced among fungal taxa in the rhizoplane niche. The rhizoplane supported species specialization with enrichment of some rare species, contributing to assimilative community assembly in the rhizoplane in all soil types. and were identified as important indicator genera of the soil-root microbiome continuum and good predictors of plant agronomic traits. The findings provide empirical evidence for host plant selection and enrichment/depletion processes of fungal microbiome assembly along the soil-root continuum. Fungal community assembly along the soil-root continuum is shaped largely by the host plant rather than the soil type. This finding facilitates the implementations of fungi-associated biocontrol and growth-promoting for specific plants in agriculture practice, regardless of the impacts from variations in geographical environments. Furthermore, the depletion of complex ecological associations in the fungal community along the soil-root continuum and the enhancement of facilitations among rhizoplane-associated fungal taxa provide empirical evidence for the potential of community simplification as an approach to target the plant rhizoplane for specific applications. The identified indicators and along the soil-root microbiome continuum are good predictors of tobacco plant agronomic traits, which should be given attention when manipulating the root-associated microbiome.
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http://dx.doi.org/10.1128/msystems.00361-22DOI Listing
August 2022

Set2 family regulates mycotoxin metabolism and virulence via H3K36 methylation in pathogenic fungus .

Virulence 2022 Dec;13(1):1358-1378

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

infects various crops with aflatoxins, and leads to aspergillosis opportunistically. Though H3K36 methylation plays an important role in fungal toxin metabolism and virulence, no data about the biological function of H3K36 methylation in virulence has been reported. Our study showed that the Set2 histone methyltransferase family, AshA and SetB, involves in morphogenesis and mycotoxin anabolism by regulating related transcriptional factors, and they are important for fungal virulence to crops and animals. Western-blotting and double deletion analysis revealed that AshA mainly regulates H3K36me2, whereas SetB is mainly responsible for H3K36me3 in the nucleus. By construction of domain deletion strain and point mutation strains by homologous recombination, the study revealed that SET domain is indispensable in mycotoxin anabolism and virulence of , and N455 and V457 in it are the key amino acid residues. ChIP analysis inferred that the methyltransferase family controls fungal reproduction and regulates the production of AFB1 by directly regulating the production of the transcriptional factor genes, including , , and amylase, through H3K36 trimethylation in their chromatin fragments, based on which this study proposed that, by H3K36 trimethylation, this methyltransferase family controls AFB1 anabolism through transcriptional level and substrate utilization level. This study illuminates the epigenetic mechanism of the Set2 family in regulating fungal virulence and mycotoxin production, and provides new targets for controlling the virulence of the fungus .The methylation of H3K36 plays an important role in the fungal secondary metabolism and virulence, but no data about the regulatory mechanism of H3K36 methylation in the virulence of have been reported. Our study revealed that, in the histone methyltransferase Set2 family, AshA mainly catalyzes H3K36me2, and involves in the methylation of H3K36me1, and SetB mainly catalyzes H3K36me3 and H3K36me1. Through domain deletion and point mutation analysis, this study also revealed that the SET domain was critical for the normal biological function of the Set2 family and that N455 and V457 in the domain were critical for AshA. By ChIP-seq and ChIP-qPCR analysis, H3K36 was found to be trimethylation modified in the promotors and ORF positions of , and the amylase gene (AFLA_084340), and further qRT-PCR results showed that these methylation modifications regulate the expression levels of these genes. According to the results of ChIP-seq analysis, we proposed that, by H3K36 trimethylation, this methyltransferase family controls the metabolism of mycotoxin through transcriptional level and substrate utilization level. All the results from this study showed that Set2 family is essential for fungal secondary metabolism and virulence, which lays a theoretical groundwork in the early prevention and treatment of pollution, and also provides an effective strategy to fight against other pathogenic fungi.
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http://dx.doi.org/10.1080/21505594.2022.2101218DOI Listing
December 2022

Growth, biofilm formation and atrazine degrading gene (trzN) expression of Arthrobacter sp. DNS10 cultured with montmorillonite, kaolinite and goethite.

Chemosphere 2022 Aug 5;307(Pt 2):135904. Epub 2022 Aug 5.

School of Resources &; Environment, Northeast Agricultural University, Harbin, 150030, PR China; Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130132, PR China. Electronic address:

The viable and degradation potential of the strains which adhered to soil minerals are essential for eliminating organic pollutants from soil. Herein, the interaction (growth, biofilm formation and survive) of Arthrobacter sp. DNS10, an atrazine degrading strain, with three kinds of typical soil minerals, such as montmorillonite, kaolinite and goethite, as well as the atrazine degradation gene (trzN) expression of the strain in the minerals system were studied. The results showed that montmorillonite had significant promotion effect on the growth of strain DNS10, followed by kaolinite, but goethite significantly inhibited the growth of strain DNS10. In contrast, goethite notably promoted the biofilm formation and there was less biofilm detected in montmorillonite containing system. The percentage of the survival bacteria in the biofilm that formed on montmorillonite, kaolinite and goethite was 53.8%, 40.8% and 28.2%. In addition, there were more reactive oxygen species (ROS) were detected in the cells that exposed to goethite than those of the cells exposed to kaolinite and montmorillonite. These results suggest that the electrostatic repulsion between kaolinite/montmorillonite and strain DNS10 prevents them from contacting each other and facilitates bacterial growth by allowing the strain to obtain more nutrients. Oppositely, the needle-like morphology of goethite might damage the strain DNS10 cell when they were combined by electrostatic attraction, and the goethite induced ROS also aggravate the cytotoxicity of goethite on strain DNS10. In addition, the relative transcription of trzN in the cells contacted with montmorillonite, kaolinite and goethite was 0.94-, 0.27- and 0.20- fold of the no mineral exposure treatment. Briefly, this research suggests that the minerals with different structure and/or physicochemical characteristics might cause various trend for the biofilm formation and degradation potential of the bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135904DOI Listing
August 2022

Single-cell transcriptomics reveals male germ cells and Sertoli cells developmental patterns in dairy goats.

Front Cell Dev Biol 2022 22;10:944325. Epub 2022 Jul 22.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Spermatogenesis holds considerable promise for human-assisted reproduction and livestock breeding based on stem cells. It occurs in seminiferous tubules within the testis, which mainly comprise male germ cells and Sertoli cells. While the developmental progression of male germ cells and Sertoli cells has been widely reported in mice, much less is known in other large animal species, including dairy goats. In this study, we present the data of single cell RNA sequencing (scRNA-seq) for 25,373 cells from 45 (pre-puberty), 90 (puberty), and 180-day-old (post-puberty) dairy goat testes. We aimed to identify genes that are associated with key developmental events in male germ cells and Sertoli cells. We examined the development of spermatogenic cells and seminiferous tubules from 15, 30, 45, 60, 75, 90, 180, and 240-day-old buck goat testes. scRNA-seq clustering analysis of testicular cells from pre-puberty, puberty, and post-puberty goat testes revealed several cell types, including cell populations with characteristics of spermatogonia, early spermatocytes, spermatocytes, spermatids, Sertoli cells, Leydig cells, macrophages, and endothelial cells. We mapped the timeline for male germ cells development from spermatogonia to spermatids and identified gene signatures that define spermatogenic cell populations, such as AMH, SOHLH1, INHA, and ACTA2. Importantly, using immunofluorescence staining for different marker proteins (UCHL1, C-KIT, VASA, SOX9, AMH, and PCNA), we explored the proliferative activity and development of male germ cells and Sertoli cells. Moreover, we identified the expression patterns of potential key genes associated with the niche-related key pathways in male germ cells of dairy goats, including testosterone, retinoic acid, PDGF, FGF, and WNT pathways. In summary, our study systematically investigated the elaborate male germ cells and Sertoli cells developmental patterns in dairy goats that have so far remained largely unknown. This information represents a valuable resource for the establishment of goat male reproductive stem cells lines, induction of germ cell differentiation , and the exploration of sequential cell fate transition for spermatogenesis and testicular development at single-cell resolution.
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http://dx.doi.org/10.3389/fcell.2022.944325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355508PMC
July 2022

Exploring Different Effects of Exclusive Enteral Nutrition (EEN) and Corticosteroids on the Gut Microbiome in Crohn's Disease Based on a Three-Stage Strategy.

Gastroenterol Res Pract 2022 27;2022:6147124. Epub 2022 Jul 27.

Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China.

The objective of this study was to compare the efficacy of exclusive enteral nutrition (EEN) and corticosteroids on the gut microbiome in Crohn's disease. . Data were collected for 16 patients newly diagnosed with CD as the test group and 10 healthy volunteers as the control group. The 16 patients were randomly divided into the EEN group and the corticosteroids group. For subsequent analysis, 6 patients in the EEN group with follow-up were enrolled to compare the 0-month, 1-month, and 3-month outcomes. We analyzed and compared gut microbiota between different groups in 3 stages. To evaluate the clinical outcome of treatment, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), hemoglobin (HB), albumin (ALB), and Crohn's disease activity (CDAI) were recorded. . There are significant differences in microbiota between patients with CD and healthy people, and there are intuitive differences in the main components of the microbiota. 16 patients were included in stage 2, in which both corticosteroids and EEN can induce CD remission well. However, corticosteroids have a greater impact on inflammatory indicators, while EEN has a more obvious effect on nutritional indicators. Principal component analysis suggests that there are different compositional changes in the gut microbiome after corticosteroids and EEN treatment. After 3 months of dynamic observation, we found that EEN can effectively maintain CD remission, reduce inflammatory indicators, and improve nutritional indicators. . Both EEN and corticosteroids can increase the diversity of the microbiome in inducing CD remission, while they have different effects on the proportion of microbiome species. This trial is registered with NCT02056418.
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http://dx.doi.org/10.1155/2022/6147124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348958PMC
July 2022

A joint constrained CCA model for network-dependent brain subregion parcellation.

IEEE J Biomed Health Inform 2022 Aug 5;PP. Epub 2022 Aug 5.

Connectivity-based brain region parcellation from functional magnetic resonance imaging (fMRI) data is complicated by heterogeneity among aged and diseased subjects, particularly when the data are spatially transformed to a common space. Here, we propose a group-guided functional brain region parcellation model capable of obtaining subregions from a target region with consistent connectivity profiles across multiple subjects, even when the fMRI signals are kept in their native spaces. The model is based on a joint constrained canonical correlation analysis (JC-CCA) method that achieves group-guided parcellation while allowing the data dimension of the parcellated regions for each subject to vary. We performed extensive experiments on synthetic and real data to demonstrate the superiority of the proposed model compared to other classical methods. When applied to fMRI data of subjects with and without Parkinson's disease (PD) to estimate the subregions in the Putamen, significant between-group differences were found in the derived subregions and the connectivity patterns. Superior classification and regression results were obtained, demonstrating its potential in clinical practice.
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http://dx.doi.org/10.1109/JBHI.2022.3196689DOI Listing
August 2022

Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6.

Biomater Adv 2022 May 1;136:212781. Epub 2022 Apr 1.

Shenzhen Beike Biotechnology Research Institute, Shenzhen 518057, PR China; Intervention and Cell Therapy Center, Shenzhen Hospital of Peking University, Shenzhen 518057, PR China. Electronic address:

Application of adipose-derived mesenchymal stromal cells (AMSCs)-derived extracellular vesicles (EVs) in skin wound healing has been documented. In this study, we investigated the therapeutic potential of AMSCs-derived EVs in skin wound healing through delivery of microRNA-10b (miR-10b). HaCaT cells were treated with HO to establish the skin wound cell models. Next, the binding affinity between miR-194, PEA15, and CDK6 was identified. Additionally, EVs were isolated from the culture medium of AMSC sheets, followed by incubation with HO-treated HaCaT cells to detect cell proliferation, migration, and apoptosis using gain- or loss-of-function experiments. Lastly, the mice skin wound models were also established to assess skin wound healing ability. miR-10b was down-regulated in the skin trauma models and enriched in the EVs of AMSC sheets. Moreover, miR-10b derived from EVs targeted PEA15 to promote CDK6 expression, thereby stimulating the proliferation and migration of HO-damaged HaCaT cells but inhibiting apoptosis. In vivo experiments further ascertained the therapeutic functionality of AMSC sheets-derived EVs-miR-10b. In summary, AMSC sheets-derived EVs carrying miR-10b promoted CDK6 expression to intensify skin wound healing by regulating PEA15.
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http://dx.doi.org/10.1016/j.bioadv.2022.212781DOI Listing
May 2022

Eudicot primary cell wall glucomannan is related in synthesis, structure and function to xyloglucan.

Plant Cell 2022 Aug 4. Epub 2022 Aug 4.

Department of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court Road, Cambridge CB2 1QW, UK.

Hemicelluose polysaccharides influence assembly and properties of the plant primary cell wall (PCW), perhaps by interacting with cellulose to affect the deposition and bundling of cellulose fibrils. However, the functional differences between plant cell wall hemicelluloses such as glucomannan, xylan and xyloglucan (XyG) remain unclear. As the most abundant hemicellulose, XyG is considered important in eudicot PCWs, but plants devoid of XyG show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM) is widespread in eudicot PCWs and shows remarkable similarities to XyG. The sugar linkages forming the backbone and side chains of β-GGM are analogous to those that make up XyG, and moreover, these linkages are formed by glycosyltransferases from the same CAZy families. Solid-state nuclear magnetic resonance indicated that β-GGM shows low mobility in the cell wall, consistent with interaction with cellulose. Although Arabidopsis β-GGM synthesis mutants show no obvious growth defects, genetic crosses between β-GGM and XyG mutants produce exacerbated phenotypes compared to XyG mutants. These findings demonstrate a related role of these two similar but distinct classes of hemicelluloses in PCWs. This work opens avenues to study the roles of β-GGM and XyG in PCWs.
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http://dx.doi.org/10.1093/plcell/koac238DOI Listing
August 2022

Case Report: Prenatal Diagnosis of Nemaline Myopathy.

Front Pediatr 2022 19;10:937668. Epub 2022 Jul 19.

Department of Ultrasound Diagnostic, The Second Xiangya Hospital, Central South University, Changsha, China.

Nemaline myopathy (NM) is a rare, hereditary heterogeneous myopathy. Fetal NM has a more severe disease course and a poorer prognosis and is usually lethal during the first few months of life. Hence, early prenatal diagnosis is especially important for clinical interventions and patient counseling. We report the case of a fetus with NM due to gene variation leading to arthrogryposis multiplex congenita (AMC). The ultrasonography and histopathology results revealed an enhanced echo intensity and decreased muscle thickness, which may be novel features providing early clues for the prenatal diagnosis of NM. Moreover, to our knowledge, this article is the first report to describe a case of NM associated with complex congenital heart disease (CHD).
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http://dx.doi.org/10.3389/fped.2022.937668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343628PMC
July 2022

Highly sensitive sensing and quantitative detection of sulfate ion with a SERS chip-based on boric acid's Lewis effect.

Anal Sci 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.

Based on the Lewis acid's coordination principle, a surface-enhanced Raman scattering (SERS) chip strategy had been developed for the ultrasensitive quantitation of SO. Through the immobilization of silver nanoparticles (Ag NPs) and the construction of the boric acid-based sensing unit, the chip system displayed outstanding merits on the direct sensing of SO, e.g., simple operation, ultra-high sensitivity, reproducibility, excellent selectivity and specificity. Moreover, an accurate evaluation was obtained by ratiometric calculations on characteristic peaks (1382 and 1070 cm) for quantitative detection of SO. The detection limit was down to 10 nM. Tap water, beer, and mineral water samples were tested, and high recoveries were achieved (97.12-110.12%). Besides, such SERS chip also displayed strong applicability for the evaluation of SO. Therefore, this SERS chip provided a promising idea for the quantification of trace amounts of SO and SO in the fields of food safety and environmental monitoring.
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http://dx.doi.org/10.1007/s44211-022-00169-5DOI Listing
August 2022

Exploring the landscape, hot topics, and trends of bariatric metabolic surgery with machine learning and bibliometric analysis.

Ther Adv Gastrointest Endosc 2022 Jan-Dec;15:26317745221111944. Epub 2022 Jul 28.

Department of Gastrointestinal Surgery, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao 266000, Shandong, China.

Background: This study aimed to analyze the landscape of publications on bariatric metabolic surgery through machine learning and help experts and scholars from various disciplines better understand bariatric metabolic surgery's hot topics and trends.

Methods: In January 2021, publications indexed in PubMed under the Medical Subject Headings (MeSH) term 'Bariatric Surgery' from 1946 to 2020 were downloaded. Python was used to extract publication dates, abstracts, and research topics from the metadata of publications for bibliometric evaluation. Descriptive statistical analysis, social network analysis (SNA), and topic modeling with latent Dirichlet allocation (LDA) were used to reveal bariatric metabolic surgery publication growth trends, landscape, and research topics.

Results: A total of 21,798 records of bariatric metabolic surgery-related literature data were collected from PubMed. The number of publications indexed to bariatric metabolic surgery had expanded rapidly. and are currently the most published journals in bariatric metabolic surgery. The bariatric metabolic surgery research mainly included five topics: bariatric surgery intervention, clinical case management, basic research, body contour, and surgical risk study.

Conclusion: Despite a rapid increase in bariatric metabolic surgery-related publications, few studies were still on quality of life, psychological status, and long-term follow-up. In addition, basic research has gradually increased, but the mechanism of bariatric metabolic surgery remains to be further studied. It is predicted that the above research fields may become potential hot topics in the future.
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http://dx.doi.org/10.1177/26317745221111944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340401PMC
July 2022

Preparation and research of epoxy modified by carboxyl-terminated polybutylene adipate at room temperature.

RSC Adv 2022 Jul 15;12(32):20471-20480. Epub 2022 Jul 15.

Department of Basic, Naval University of Engineering Wuhan 430033 China

In this study, carboxyl-terminated polybutylene adipate (CTPBA) was used to modify epoxy resin, and the modified epoxy resin was cured by a room temperature rapid curing agent (T-31). The effects of CTPBA modification on bonding properties and mechanical properties of epoxy resin adhesive at room temperature were carefully studied. Epoxy-terminated prepolymer was synthesized by pre-polymerization and its structure was characterized. Compared with the addition method of direct blending, the bonding properties and mechanical properties of pre-polymerized epoxy resin adhesive were significantly better. Compared with unmodified epoxy resin, CTPBA modification significantly improved the bonding strength. Furthermore, with the increase of CTPBA content, the shear strength of the material increased first and then decreased, and reached the maximum when the addition amount was 40 phr. This shows that the tensile strength of the material decreased with the increase of CTPBA content, and the elongation at break increased with the increase of CTPBA content. Dynamic mechanical analyzer (DMA) test results showed that the addition of CTPBA reduced the glass transition temperature, but broadened the damping temperature range. TG analysis showed that the thermal stability of the modified epoxy resin was good, and compared with pure epoxy resin, the initial temperature of thermal weight loss and the maximum thermal decomposition rate decreased, but the overall thermal stability was not significantly different. In summary, CTPBA modification of epoxy resin is expected to improve the comprehensive mechanical properties at room temperature.
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http://dx.doi.org/10.1039/d2ra02915dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284665PMC
July 2022

Toxoplasma gondii dense granule protein 3 promotes endoplasmic reticulum stress-induced apoptosis by activating the PERK pathway.

Parasit Vectors 2022 Aug 2;15(1):276. Epub 2022 Aug 2.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, China.

Background: Toxoplasma gondii is a neurotropic single-celled parasite that can infect mammals, including humans. Central nervous system infection with T. gondii infection can lead to Toxoplasma encephalitis. Toxoplasma infection can cause endoplasmic reticulum (ER) stress and unfolded protein response (UPR) activation, which ultimately can lead to apoptosis of host cells. The dense granule protein GRA3 has been identified as one of the secretory proteins that contribute to the virulence of T. gondii; however, the mechanism remains enigmatic.

Methods: The expression of the GRA3 gene in RH, ME49, Wh3, and Wh6 strains was determined using quantitative real-time polymerase chain reaction (qRT-PCR). pEGFP-GRA3 was constructed by inserting Chinese 1 Wh6 GRA3 (GRA3) cDNA into a plasmid encoding the enhanced GFP. Mouse neuro2a (N2a) cells were transfected with either pEGFP or pEGFP-GRA3 (GRA3) and incubated for 24-36 h. N2a cell apoptosis and ER stress-associated proteins were determined using flow cytometry and immunoblotting. Furthermore, N2a cells were pretreated with GSK2656157 (a PERK inhibitor) and Z-ATAD-FMK (a caspase-12 inhibitor) before GRA3 transfection, and the effect of the inhibitors on GRA3-induced ER stress and apoptosis were investigated.

Results: GRA3 gene expression was higher in the less virulent strains of type II ME49 and type Chinese 1 Wh6 strains compared with the virulent strains of type I RH strain and type Chinese 1 Wh3 strain. Transfection with GRA3 plasmid induced neuronal apoptosis and increased the expression of GRP78, p-PERK, cleaved caspase-12, cleaved caspase-3, and CHOP compared with the control vector. Pretreatment with GSK2656157 and Z-ATAD-FMK decreased apoptosis in N2a cells, and similarly, ER stress- and apoptosis-associated protein levels were significantly decreased.

Conclusion: GRA3 induces neural cell apoptosis via the ER stress signaling pathway, which could play a role in toxoplasmic encephalitis.
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http://dx.doi.org/10.1186/s13071-022-05394-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344675PMC
August 2022

Gas permeation through nanoporous single-walled carbon nanotubes: The confinement effect.

Nanotechnology 2022 Aug 2. Epub 2022 Aug 2.

Shanghai Institute of Applied Mathematics and Mechanics, Shanghai University, Shanghai Institute of Applied Mathematics and Mechanics, Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai University, Shanghai 200072, People's Republic of China, Shanghai, 200072, CHINA.

The gas permeation through nanoscale membranes like graphene has been extensively studied by experiments and empirical models. In contrast to planar membranes, the single-walled carbon nanotube has a natural confined hollow structure, which shall affect the gas permeation process. We perform molecular dynamics simulations to investigate the effect of the nanotube diameter on the gas permeation process. It is found that the permeance constant increases with the increase of the nanotube diameter, which can not be explained by existing empirical models. We generalize the three-state model to describe the diameter dependence for the permeance constant, which discloses a distinctive confinement-induced adsorption phenomenon for the gas molecule on the nanotube's inner surface. This adsorption phenomenon effectively reduces the pressure of the bulk gas, leading to the decrease of the permeance constant. These results illustrate the importance of the adsorption within the confined space on the gas permeation process.
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http://dx.doi.org/10.1088/1361-6528/ac85f5DOI Listing
August 2022

Sodium butyrate reduces overnutrition-induced microglial activation and hypothalamic inflammation.

Int Immunopharmacol 2022 Jul 30;111:109083. Epub 2022 Jul 30.

Medical Research Center, Affiliated Hospital 2 of Nantong University, Nantong 226001, People's Republic of China; Department of Pathogen Biology, Medical College, Nantong University, Nantong 226001, People's Republic of China; Nantong Key Laboratory of Molecular Immunology, Affiliated Hospital 2 of Nantong University, Nantong 226001, People's Republic of China. Electronic address:

Overnutrition-induced hypothalamic inflammation greatly disturbs feeding behavior and energy homeostasis as well as the pathogenesis of obesity. Butyrate, a short-chain fatty acid, reportedly participates in the regulation of the immune response and energy metabolism in the body. However, the role of butyrate in overnutrition-induced microglial activation and hypothalamic inflammation remains unclear. In the present study, we established a high-fat diet (HFD)-induced hypothalamic inflammation model in mice. Oral supplementation with sodium butyrate (NaB) significantly reduced HFD-induced microgliosis, inflammatory cytokine expression, endoplasmic reticulum (ER) stress, neuronal apoptosis, and neuropeptide Y (NPY) expression in the mouse hypothalamus. Utilizing a high-glucose (HG)-stimulated microglial activation model in vitro, we found that NaB inhibited the HG-induced expression of the inflammatory factor IL-1β. Moreover, NaB exerted an antioxidant effect by balancing HO-1 and NOX4 expression, thus preventing reactive oxygen species (ROS) production in HG-treated microglia. Interestingly, NaB treatment promoted microglial process formation and extension via the Akt/Cdc42 pathway under both normal and HG-stimulated conditions, indicating a resting morphology of microglia. Taken together, our study revealed for the first time the anti-inflammatory and antioxidant effects of NaB in overnutrition-induced microglial activation and hypothalamic inflammation, which might become a potential therapeutic option for obesity prevention and treatment.
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http://dx.doi.org/10.1016/j.intimp.2022.109083DOI Listing
July 2022

In-silico study of reducing human health risk of POP residues' direct (from tea) or indirect exposure (from tea garden soil): Improved rhizosphere microbial degradation, toxicity control, and mechanism analysis.

Ecotoxicol Environ Saf 2022 Jul 31;242:113910. Epub 2022 Jul 31.

College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, China.

The accumulation of potentially harmful substances in tea garden soils and tea leaves, especially persistent organic pollutants (POPs), is a special concern for tea consumers worldwide. However, their potential health and ecological risks in tea gardens have rarely been investigated. This study proposed measures to improve the degradation ability of POPs by the tea rhizosphere and to reduce the human health risks caused by POPs after tea consumption. In this study, the binding energy values of six types of POPs and the degraded protein were used to reflect the degradation ability and calculated using molecular dynamic simulations. The main root secretions (i.e., catechin, glucose, arginine, and oxalic acid) were selected and applied with a combination of tea fertilizer and trace element combination (i.e., urea, straw, and copper element), leading to an improved degradation ability (49.59 %) of POPs. To investigate the mechanisms of the factors that affect the degradation ability, molecular docking, tensor singular value decomposition methods, multivariate correlation analysis and 2D-QSAR model were used. The results showed that the solvation energy and solvent accessible surface area are the main forces, and the molecular weight, boiling point, and topological radius of the POPs were the key molecular features affecting their degradation ability. Based on the three key characteristics, a diet avoidance scheme (i.e., avoiding lysine, maslinic acid, ethanol, perfluorocaproic acid, and cholesterol with tea), which can reduce the binding ability of POP residues to aromatic hydrocarbon receptors by 506.13 %. This work will provide theoretical strategies to improve the quality and safety of tea production and reduce the potential risks of harmful substance residues in tea garden soils and tea leaves.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113910DOI Listing
July 2022

Phase Conversion Accelerating "Zn-Escape" Effect in ZnSe-CFs Heterostructure for High Performance Sodium-Ion Half/Full Batteries.

Small 2021 Nov 28:e2105169. Epub 2021 Nov 28.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan, Hubei, 430070, China.

Sodium-ion batteries (SIBs) are considered as a promising large-scale energy storage system owing to the abundant and low-cost sodium resources. However, their practical application still needs to overcome some problems like slow redox kinetics and poor capacity retention rate. Here, a high-performance ZnSe/carbon fibers (ZnSe-CFs) anode is demonstrated with high electrons/Na transport efficiency for sodium-ion half/full batteries by engineering ZnSe/C heterostructure. The electrochemical behavior of the ZnSe-CFs heterostructure anode is deeply studied via in situ characterizations and theoretical calculations. Phase conversion is revealed to accelerate the "Zn-escape" effect for the formation of robust solid electrolyte interphase (SEI). This leads to the ZnSe-CFs delivering a superior rate performance of 206 mAh g at 1500 mA g for half battery and an initial discharge capacity of 197.4 mAh g at a current density of 1 A g for full battery. The work here heralds a promising strategy to synthesize advanced heterostructured anodes for SIBs, and provides the guidance for a better understanding of phase conversion anodes.
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http://dx.doi.org/10.1002/smll.202105169DOI Listing
November 2021

Multi-focus image fusion with enhancement filtering for robust vascular quantification using photoacoustic microscopy.

Opt Lett 2022 Aug;47(15):3732-3735

Accurate identification and quantification of microvascular patterns are important for clinical diagnosis and therapeutic monitoring using optical-resolution photoacoustic microscopy (OR-PAM). Due to its limited depth of field, conventional OR-PAM may not fully reveal microvascular patterns with enough details in depth range, which affects the segmentation and quantification. Here, we propose a robust vascular quantification approach via combining multi-focus image fusion with enhancement filtering (MIFEF). The multi-focus image fusion is constructed based on multi-scale gradients and image matting to improve image fusion quality by considerably achieving accurate focus measurement for initial segmentation as well as decision map refinement. The enhancement filtering identifies the vessels and handles noise without deforming microvasculature. The performance of the MIFEF were evaluated employing a leaf phantom, mouse livers and brains. The proposed method for OR-PAM can significantly facilitate the clinical provision of optical biopsy of vascular-related diseases.
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http://dx.doi.org/10.1364/OL.459629DOI Listing
August 2022

Metformin Inhibits HaCaT Cell Proliferation Under Hyperlipidemia Through Reducing Reactive Oxygen Species via FOXO3 Activation.

Clin Cosmet Investig Dermatol 2022 22;15:1403-1413. Epub 2022 Jul 22.

Department of Dermatology, The First Medical Center of Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.

Purpose: Metformin (MET) has been proved to be effective for the treatment of psoriasis. The mechanisms of its action under the hyperlipidemia have yet to be fully elucidated. Here, we investigated the effect of metformin on the cell proliferation induced by hyperlipidemia and the underlying mechanism in immortalized human keratinocyte cell line (HaCat).

Methods: Wild-type or FOXO3 knockdown HaCat cells were treated with free fatty acids (FFA) for 10 days and then co-treated with metformin for another 4 days. Triglyceride (TG) level, cell viability, proliferation, apoptosis, antioxidant enzymes, reactive oxygen species (ROS) levels, as well as the transcription activity of FOXO3 were analyzed.

Results: Metformin decreased HaCaT cell proliferation and induced cell apoptosis after FFA treatment. Metformin was found to significantly increase the expressions and the activities of superoxide dismutase (SOD) as well as catalase (CAT), and reduced the reactive oxygen species (ROS) level. Metformin significantly promoted the autophagy and increase FOXO3 protein level in the nucleus under hyperlipidemia. However, all of the effects from metformin were partially blocked by FOXO3 knockdown.

Conclusion: This study demonstrated that under the hyperlipidemia, metformin has significant antiproliferation and proapoptosis effects by reducing ROS level as well as increasing autophagy. All of these effects from metformin were through FOXO3-dependent pathway.
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http://dx.doi.org/10.2147/CCID.S368845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326038PMC
July 2022

Light-Assisted Semi-Hydrogenation of 1,3-Butadiene with Water.

Angew Chem Int Ed Engl 2022 Jul 31. Epub 2022 Jul 31.

University of Namur: Universite de Namur, Chemistry, 61 rue de Bruxelles, 5000, Namur, BELGIUM.

Sustainable processes for semi-hydrogenation of alkynes/alkadienes impurities in alkenes feedstocks are in great demand in industry as the utilization of excessive hydrogen, high temperature and unsatisfactory alkenes selectivity of the current thermo-catalytic route, however, their development is still challenging. Herein, we innovate a light-assisted semi-hydrogenation process in gas-feed fixed bed reactor, with water as hydrogen atom source by in-situ photocatalysis. Using Pd/TiO 2 as model catalyst, this process shows an excellent catalytic performance for the semi-hydrogenation of 1,3-butadiene, with 100% of butenes selectivity at ~99% of conversion over 180 h of reaction at ambient temperature driven by 66 mW cm -2 of irradiation intensity . This light-driven, H 2 -free, ambient temperature semi-hydrogenation process, with superior performance to that of thermocatalytic route, shows attractive to bring an evolution i n industrial hydrogenation technology to an economical and safe way .
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http://dx.doi.org/10.1002/anie.202210573DOI Listing
July 2022

A novel steady-state model to quantitively assess the effect of pH elevation by dissimilatory sulfate reduction process in acidic waters in mining areas.

Water Res 2022 Jul 12;222:118852. Epub 2022 Jul 12.

Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, School of Environmental Science & Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Acidic waters such as groundwater, drainage and lakes in mining area contain high-strength acids and metal ions, posing serious threats to aquatic ecosystems and human health. Dissimilatory sulfate reduction (DSR)-based processes are attractive technologies for remediating acidic waters because it produces alkalinity and sulfide for metal precipitation and acid neutralization. However, the effects of pH elevation achieved by DSR-based processes are case-sensitive and difficult to be quantitively assessed, which limits the application of DSR process for acidic water remediation. Therefore, in this study, a Sulfidogenic Acid mine water Remediation Model (SARM) considering the DSR process, weak acids balance, metal sulfide and hydroxide precipitations, and gas-liquid exchanges of HS and CO, was developed to quantitatively assess the effects of various environmental factors on the pH elevation by a DSR process in acidic waters. A long-term trial of a DSR reactor was conducted to calibrate and validate the SARM. The experimental results revealed that the DSR-based process is effective to relieve acidity. The calibrated SARM demonstrated the excellent performance to predict the pH variation in the DSR reactor, under the varied conditions of influent pH and organic concentration. The calibrated SARM was further validated with data collected from literatures, and the results verified that the proposed model is capable to accurately assess the effect of DSR process on acid neutralization and metal removals under various conditions in steady state. The model was employed to systematically evaluate the impacts of environmental factors on acid remediation within a DSR-based process. The results revealed that the background alkalinity plays an important role in acid neutralization. However, with an increase in sulfate reduction, biogenic sulfide and carbonate become the dominant buffering substances to neutralize acidity. Furthermore, the SARM was used to evaluate the applicability of the DSR-based process for the remediation of acidic waters by evaluating the sulfide production thresholds for acid neutralization and metal removal. The simulation results demonstrated that, the DSR-based process is recommended for the remediation of acidic waters with low background alkalinity. Collectively, the SARM proposed in this study was found to be a useful and efficient tool for quantitatively assessing the potential of DSR-based processes for neutralizing acidic waters, which is vital for biogeochemistry and environmental engineering research.
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http://dx.doi.org/10.1016/j.watres.2022.118852DOI Listing
July 2022

Topical 5-aminolevulinic acid photodynamic therapy for cervical high-grade squamous intraepithelial lesions.

Photodiagnosis Photodyn Ther 2022 Jul 26;39:103037. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No.600 Yishan Road, Shanghai 200233, China. Electronic address:

Objective: To evaluate the efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in the treatment of high-grade squamous intraepithelial lesions of the cervix (HSIL).

Methods: This retrospective study included 22 female patients with histologically confirmed HSIL and high-risk human papillomavirus (HR-HPV) infections. Patients were treated with ALA-PDT once a week for a total of 6 times. All patients had a follow-up period of 3 months and 6 months. The assessment of effectiveness of ALA-PDT was performed by ThinPrep cytology test (TCT), HPV DNA assay, HPV E6/E7 mRNA examination, colposcopy, biopsy, and immunohistochemistry detection.

Results: Three months after 5-ALA PDT, the histologic disappearance rate was 81.82% (18/22), while the HPV clearance rate was 54.55% (12/22). At the 6 months checkpoint, the HSIL disappearance rate was 90.91% (20/22) and the HPV clearance rate was 86.36% (19/22). Before PDT, 68.18% of patients (15/22) were confirmed with TCT abnormalities, while only one patient (1/22, 4.55%) was abnormal in the TCT test at 6 months checkpoint. All participants were found HPV E6/E7 mRNA positive initially, while the HPV E6/E7 mRNA negative rate was 90.91% (20/22) at 6 months checkpoint. Additionally, we found a significant difference of the expression of CD4+ and CD8+ T cells and HPV E6 and E7 proteins before ALA-PDT and at 3 months follow-up (P < 0.01). No severe side effects were seen.

Conclusions: Topical 5-ALA PDT is an effective treatment for cervical HSIL with HR-HPV infection.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103037DOI Listing
July 2022

Specific extraction of nucleic acids employing pillar[6]arene-functionalized nanochannel platforms.

Chem Commun (Camb) 2022 Jul 29. Epub 2022 Jul 29.

State Key Laboratory of Food Science and Technology, Jiangnan University, China.

The rapid extraction of high-purity nucleic acids from complex biological samples using conventional methods is complicated. Therefore, in this study, glycine-pillar[6]arene (Gly-P6)-functionalized tapered nanochannels were constructed using 32-mer single-stranded . DNA (ssDNA) as a model sequence, which can selectively transport ssDNA by multiple noncovalent forces (transport flux of 2.65 nM cm h) under the interference of amino acids and other substances. In view of these prospective results, the selective transport of nucleic acids with nanochannels could be applied in the design of nucleic acid enrichment and separation systems in the future.
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http://dx.doi.org/10.1039/d2cc02693gDOI Listing
July 2022

Comparative physiological and transcriptome analysis reveals the potential mechanism of selenium accumulation and tolerance to selenate toxicity of Broussonetia papyrifera.

Tree Physiol 2022 Jul 28. Epub 2022 Jul 28.

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China.

Broussonetia papyrifera is an important fodder tree that is widely distributed in China. Enhancing the selenium (Se) content in B. papyrifera may help to improve the nutritional value of the feed. In this study, sodium selenite and selenate were foliar-applied to investigate the mechanisms of Se tolerance and accumulation in B. papyrifera. The results showed that both Se forms significantly increased the total Se content, and the proportion of organic Se was significantly higher in the sodium selenite treatment than in the control. In addition, the soluble sugar, phenolic acid, and flavonoid contents and antioxidant enzyme activities were increased by exogenous Se. The de novo RNA sequencing results showed that 644 and 1804 differentially expressed genes were identified in the selenite and selenate comparison groups, respectively. Pathway enrichment analysis demonstrated that 24 of the 108 pathways were significantly enriched, of which sulfur assimilation genes in the sodium selenite-treated groups were upregulated, whereas Se conjugation and transporter genes, such as SBP1, PCS, GSTs, ABCs, and GPX, were significantly induced under selenate treatment. The hub genes identified by weighted-gene co-expression network analysis further confirmed that sulfur assimilation, conjugation, and transporter genes might play a vital role in Se assimilation and tolerance. From this, a model of Se metabolism in B. papyrifera was proposed based on the above physiological and RNA sequencing data. This study is the first study to report that B. papyrifera has a strong ability to accumulate and tolerate exogenous Se, thereby providing a foundation for further characterization of the accumulation and tolerance mechanism of B. papyrifera. Our findings can provide technical support for producing Se-enriched fodder.
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http://dx.doi.org/10.1093/treephys/tpac095DOI Listing
July 2022

A Machine Vision Approach for Classification of Skin Cancer Using Hybrid Texture Features.

Comput Intell Neurosci 2022 18;2022:4942637. Epub 2022 Jul 18.

Department of Computer Science, School of Systems & Technology, University of Management and Technology, Lahore, Pakistan.

The main purpose of this study is to observe the importance of machine vision (MV) approach for the identification of five types of skin cancers, namely, actinic-keratosis, benign, solar-lentigo, malignant, and nevus. The 1000 (200 × 5) benchmark image datasets of skin cancers are collected from the International Skin Imaging Collaboration (ISIC). The acquired ISIC image datasets were transformed into texture feature dataset that was a combination of first-order histogram and gray level co-occurrence matrix (GLCM) features. For the skin cancer image, a total of 137,400 (229 × 3 x 200) texture features were acquired on three nonover-lapping regions of interest (ROIs). Principal component analysis (PCA) clustering approach was employed for reducing the dimension of feature dataset. Each image acquired twenty most discriminate features based on two different approaches of statistical features such as average correlation coefficient plus probability of error (ACC + POE) and Fisher (Fis). Furthermore, a correlation-based feature selection (CFS) approach was employed for feature reduction, and optimized 12 features were acquired. Furthermore, a classification algorithm naive bayes (NB), Bayes Net (BN), LMT Tree, and multilayer perception (MLP) using 10 K-fold cross-validation approach were employed on optimized feature datasets and the overall accuracy achieved by MLP is 97.1333%.
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http://dx.doi.org/10.1155/2022/4942637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313960PMC
July 2022
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