Publications by authors named "Yu Lan"

789 Publications

Effects of biochar on NO emission in denitrification pathway from paddy soil: A drying incubation study.

Sci Total Environ 2021 May 8;787:147591. Epub 2021 May 8.

Liaoning Biochar Engineering & Technology Research Center, Shenyang Agricultural University, Shenyang 110866, China. Electronic address:

NO emission from paddy soil is a potential environmental risk, especially when the soil moisture content of paddy soil changes and excessive nitrogen retention occurs. Biochar is known to have a positive effect on reducing NO emissions. However, the influence of different types of biochar on NO emission with varying soil moisture contents is unclear. The objective of this study was to investigate the effects of biochar made from different feedstocks and at different pyrolysis temperatures on the release of NO during drying process of paddy soil. An incubation experiment with four kinds of biochar (rice straw and rice husk biochar pyrolyzed at 400 °C and 700 °C, respectively) applied at 1% (w/w) was conducted on paddy soil with the same initial moisture content (105% water-filled pore space). The emission rate of NO, concentrations of ammonium and nitrate, and the abundance of NO related microbial functional genes (narG and nosZ) were monitored throughout the incubation period. Biochar amendments reduced cumulative NO emissions by 56.8-90.1% compared to the control. Low-temperature rice straw biochar decreased nosZ gene abundance, downregulated the denitrification pathway, and reduced nitrogen loss and NO emission. The low-temperature pyrolysis rice husk biochar and the control showed similar trends in narG and nosZ gene abundance and NO emission. The high-temperature pyrolysis of rice straw and rice husk biochar showed opposite trends in narG gene abundance, but both increased nosZ gene abundance at the later incubation period. Different feedback on denitrification-derived NO emission in biochar application was revealed in this study by establishing a link between biotic and abiotic factors, showing that caution should be exercised when considering the use of biochar to mitigate NO emission under drying soil conditions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147591DOI Listing
May 2021

Kinetics, pathways and toxicity of hexabromocyclododecane biodegradation: Isolation of the novel bacterium Citrobacter sp. Y3.

Chemosphere 2021 Jul 15;274:129929. Epub 2021 Feb 15.

School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China.

This research investigated the biodegradation kinetics, pathways and ecological risk of hexabromocyclododecane (HBCD) by a novel bacterium Citrobacter sp. Y3. Results showed the biodegradation followed a first-order model. The specific degradation rate constant of HBCD were obviously higher in batch experiments with combined carbon sources (k: 0.156-0.290 d) than those using HBCD as the sole carbon source (k: 0.055 d). Correspondingly, the degradation half-life became much shorter (T: 2.39-4.44 d vs T: 13.7 d). HBCD could be degraded through dehydrobromination and dehalohydroxylation, of which six possible degradation products were detected. To evaluate the ecological risk of HBCD biodegradation products, acute toxicity tests were assessed for the first time. The acute toxicity decreased slowly during treatment for 3-5 d and then decreased sharply. In general, treatment by Strain Y3 is not only a biodegradation process but also a detoxification process, thus it shows potential for bioremediation of HBCD contaminated sites.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129929DOI Listing
July 2021

Oxidation of Pd(II) with disilane in a palladium-catalyzed disilylation of aryl halides: a theoretical view.

Dalton Trans 2021 May 11. Epub 2021 May 11.

School of Chemistry and Chemical Engineering, and Chongqing Key Laboratory of Theoretical and Computational Chemistry, Chongqing University, Chongqing 400030, P. R. China. and Green Catalysis Center, and College of Chemistry Zhengzhou University Zhengzhou, Henan 450001, China.

Density functional theory (DFT) calculation has been used to reveal the mechanism of the Pd-catalyzed disilylation reaction of aryl halides. The DFT calculations indicate that the reaction starts with the oxidative addition of the C-I bond to the Pd(0) catalyst. Concerted metalation-deprotonation (CMD) can then generate a five-membered palladacycle. Insertion of Pd(ii) into the Si-Si bond in disilane followed by two sequential steps of reductive eliminations yields the disilylation product and regenerates the Pd(0) catalyst. According to the NPA charge analysis along the reaction coordinates, the formal oxidative addition of the Si-Si bond to palladium could be considered as the insertion of palladium into the Si-Si bond. However, the conventional oxidative addition of the C-I bond to palladium is exactly an oxidation process with the electron transfer from the palladium atom to the C-I bond. Therefore, electron rich Pd(0) is beneficial for the oxidation process, and Pd(ii) prone to acquire electrons is beneficial for the insertion process.
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http://dx.doi.org/10.1039/d1dt00399bDOI Listing
May 2021

A Systematic Analysis on COVID-19 Patients in Inner Mongolia Based on Dynamic Monitoring.

Biomed Res Int 2021 20;2021:5559187. Epub 2021 Apr 20.

Department of Pulmonary and Critical Care Medicine/Key Laboratory of National Health Commission for the Diagnosis & Treatment of COPD, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China.

COVID-19 has spread globally with over 90,000,000 incidences and 1,930,000 deaths by Jan 11, 2021, which poses a big threat to public health. It is urgent to distinguish COVID-19 from common pneumonia. In this study, we reported multiple clinical feature analyses on COVID-19 in Inner Mongolia for the first time. We dynamically monitored multiple clinical features of all 75 confirmed COVID-19 patients, 219 pneumonia patients, and 68 matched healthy people in Inner Mongolia. Then, we studied the association between COVID-19 and clinical characteristics, based on which to construct a novel logistic regression model for predicting COVID-19. As a result, among the tested clinical characteristics, WBC, hemoglobin, C-reactive protein (CRP), ALT, and Cr were significantly different between COVID-19 patients and patients in other groups. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve was 0.869 for the logistic regression model using multiple factors associated with COVID-19. Furthermore, the CRP reaction showed five different time-series patterns with one-peak and double-peak modes. In conclusion, our study identified a few clinical characteristics significantly different between COVID-19 patients and others in Inner Mongolia. The features can be used to establish a reliable logistic regression model for predicting COVID-19.
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http://dx.doi.org/10.1155/2021/5559187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059476PMC
April 2021

Integrative transcriptomic analysis of developing hematopoietic stem cells in human and mouse at single-cell resolution.

Biochem Biophys Res Commun 2021 Jun 27;558:161-167. Epub 2021 Apr 27.

Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong, 510632, China. Electronic address:

Current understanding of hematopoietic stem cell (HSC) development comes from mouse models is considered to be evolutionarily conserved in human. However, the cross-species comparison of the transcriptomic profiles of developmental HSCs at single-cell level is still lacking. Here, we performed integrative transcriptomic analysis of a series of key cell populations during HSC development in human and mouse, including HSC-primed hemogenic endothelial cells and pre-HSCs in mid-gestational aorta-gonad-mesonephros (AGM) region, and mature HSCs in fetal liver and adult bone marrow. We demonstrated the general similarity of transcriptomic characteristics between corresponding cell populations of the two species. Of note, one of the previously transcriptomically defined hematopoietic stem progenitor cell (HSPC) populations with certain arterial characteristics in AGM region of human embryos showed close transcriptomic similarity to pre-HSCs in mouse embryos. On the other hand, the other two HSPC populations in human AGM region displayed molecular similarity with fetal liver HSPCs, suggesting the maturation in AGM before HSCs colonizing the fetal liver in human, which was different to that in mouse. Finally, we re-clustered cells based on the integrated dataset and illustrated the evolutionarily conserved molecular signatures of major cell populations. Our results revealed transcriptomic conservation of critical cell populations and molecular characteristics during HSC development between human and mouse, providing a resource and theoretic basis for future studies on mammalian HSC development and regeneration by using mouse models.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.058DOI Listing
June 2021

Biomimetic Carbene Cascades Enabled Imine Derivative Migration from CarbeneBearing Thiocarbamates.

Org Lett 2021 May 27;23(9):3518-3523. Epub 2021 Apr 27.

Institute of Next Generation Matter Transformation, College of Chemical Engineering and College of Material Sciences Engineering at Huaqiao University, 668 Jimei Boulevard, Xiamen, Fujian 361021, P. R. China.

Inspired by the body circulation of Omeprazole (irreversible proton pump inhibitor), we disclose the carbene-triggered cascades for the synthesis of 2-aminobenzofuran derivatives from -sulfonyl-1,2,3-triazoles or benzothioazole-bearing thiocarbamates, which represents an unprecedented imine derivative migration process. Furthermore, the desulfurizing reagent-free Barton-Kellogg-type reactions starting from -sulfonyl-1,2,3-triazoles have also been achieved for the first time, and elemental sulfur is confirmed as a byproduct during this transformation. Both experimental data and DFT calculations further thoroughly explained the unique reactivity.
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http://dx.doi.org/10.1021/acs.orglett.1c00930DOI Listing
May 2021

How air pollution lowers the domestic value-added ratio in exports: an empirical study of China.

Environ Sci Pollut Res Int 2021 Apr 26. Epub 2021 Apr 26.

School of Economics and Management, Nanjing Agricultural University, No.1 Weigang Road, Nanjing, 210095, People's Republic of China.

This paper analyzes the theoretical mechanism and transmission channel for the impact of air pollution on firms' domestic value-added ratio (DVAR) in exports. Based on the matched Chinese Industrial Enterprises Database and China Customs Enterprise Database, the DVAR in exports is measured, and this mechanism is empirically tested with standard measurement methods. The study concludes that air pollution is not conducive to raising the DVAR during the sample period. This conclusion remains robust to many issues, such as endogeneity, measurement error, extension of the sample interval, substitution of the air pollution index, and policy changes. The impact of air pollution on the DVAR varies by the type of firm ownership, size, and age, as well as the size and location of the city. In addition, the negative impact of air pollution on the DVAR grows with increases in the PM2.5 concentration. The growing factor prices of domestic intermediate goods and the falling productivity due to air pollution are important transmission channels for the negative impact of air pollution on the DVAR. In order for firms to open up on all fronts through quality trade development, we recommend the prevention and control of air pollution, fueling internal driving forces for firms' independent innovation, and encouraging firms to target the medium- and high-end markets.
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http://dx.doi.org/10.1007/s11356-021-14120-0DOI Listing
April 2021

Radiofrequency ablation versus total thyroidectomy in patients with papillary thyroid microcarcinoma located in the isthmus: a retrospective cohort study.

Int J Hyperthermia 2021 ;38(1):708-714

Department of Ultrasound, First Medical Center of General Hospital of Chinese PLA, Beijing, China.

Objective: This retrospective study aimed to examine the benefits and complications of radiofrequency ablation (RFA) in patients with papillary thyroid microcarcinoma (PTMC) in the isthmus.

Methods: This retrospective study included patients with PTMC in the isthmus and treated at the Chinese People's Liberation Army hospital from 05/2014 to 05/2018. The patients were divided into the RFA and total thyroidectomy (TT) groups. The outcomes were operation-related complications, rate of recurrence, metastasis rate, and thyroid carcinoma-specific questionnaire of quality of life (THYCA-QOL).

Results: Among 218 patients, 115 patients underwent RFA, and 103 underwent TT. The rates of disappearance of the ablation zone at 1, 3, 6, 12, and 18 months after RFA were 0.8% (1/115), 10.4% (12/115), 51.3% (59/115), 90.4% (104/115), and 100% (115/115), respectively. Surgical time, blood loss, hospital stays, and treatment costs were higher with TT than with RFA (all  < 0.001). The final THYCA-QOL score of the RFA group was significantly higher than in the TT group ( < 0.001). Minor pain at the operation site was seen in all patients in the RFA group. No distant metastasis was detected in all patients, but one patient in the RFA group had a recurrence after 6 months. The final THYCA-QOL score of the RFA group was significantly lower than in the TT group ( < 0.001).

Conclusion: These results suggest that RFA for PTMC in the isthmus had similar outcomes than TT. It will have to be confirmed in future studies.
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http://dx.doi.org/10.1080/02656736.2021.1916625DOI Listing
January 2021

-Repression of Expression Affects -Mediated Gene Expression in Palate Development.

Front Cell Dev Biol 2021 8;9:665109. Epub 2021 Apr 8.

Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.

Disruption of , encoding a member of the Forkhead family transcription factors, has been associated with cleft palate in humans and mice. is located in a conserved gene cluster containing , , and . We found that expression of is dramatically upregulated in the embryonic palatal mesenchyme in mouse embryos. We show here that the promoter-deletion mutation caused dramatically increased expression of the -linked allele but had little effect on the allele in . We analyzed effects of the mutation on the expression of other neighboring genes and compared those effects with the chromatin domain structure and recently identified enhancer-promoter associations as well as H3K27ac ChIP-seq data. We show that the mutation resulted in significantly increased expression of the and genes located in the same topologically associated domain with but not the expression of the and genes located in the adjacent chromatin domain. We inactivated the gene in mice homozygous for a conditional allele using CRISPR genome editing and generated mice with loss-of-function mutations in and in . Whereas the mice exhibited cleft palate at birth similar as in the mice, systematic expression analyses of a large number of Foxf2-dependent genes revealed that the (/ embryos exhibited distinct effects on the domain-specific expression of several important genes, including , , and , in the developing palatal shelves compared with embryos. These results identify a novel -regulatory effect of the mutation and demonstrate that regulation of contributed to alterations in palatal gene expression in embryos. These results have important implications for interpretation of results and mechanisms from studies of promoter- or gene-deletion alleles. In addition, the unique mouse lines generated in this study provide a valuable resource for understanding the cross-regulation and combinatorial functions of the and genes in development and disease.
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http://dx.doi.org/10.3389/fcell.2021.665109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060495PMC
April 2021

Gastric Juice-Based Genotypic Methods for Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance Testing: A Systematic Review and Meta-analysis.

Turk J Gastroenterol 2021 Jan;32(1):53-65

Department of Neurology, Beijing Haidian Hospital, Beijing, China.

Objective: To evaluate the diagnostic efficacy of gastric juice-based genotypic methods for Helicobacter pylori detection and antibiotic resistance testing.

Methods: We used electronic databases including Medline, Embase, Web of Science and the Cochrane Central Register of Controlled Trial for literature survey using keywords such as "gastric juice", "Helicobacter pylori" and their synonyms. The quality of the studies was assessed using QUADAS-2. Summary performance measures (sensitivity, specificity, positive predictive values, negative predictive values, diagnostic odds ratio, and area under the summary receiver operating characteristic curve) and HSROC curves were produced. In addition, fagan plots were applied to illustrate the relationship among the prior test probability, PLR/NLR, and posterior test probability.

Results: Our study cohort comprised eight studies with 1235 participants (617 participants of H. pylori infection and 618 participants of non-H. pylori infection). Pooled sensitivity and specificity with a corresponding 95% confidence interval (CI) of gastric juice-based genotypic methods reflected values of 94% (95%CI, 86% - 98%) and 98% (95%CI, 85% - 100%), respectively. The global sensitivity and specificity of clarithromycin resistance were 92% (95%CI, 85% - 96%) and 90% (95%CI, 80% - 95%), respectively.

Conclusion: Gastric juice-based genotypic methods can be used for diagnostic prediction of H. pylori infection as well as clarithromycin resistance testing.
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http://dx.doi.org/10.5152/tjg.2020.20025DOI Listing
January 2021

The beneficial effects of the composite probiotics from camel milk on glucose and lipid metabolism, liver and renal function and gut microbiota in db/db mice.

BMC Complement Med Ther 2021 Apr 22;21(1):127. Epub 2021 Apr 22.

College of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi, 830011, China.

Background: Probiotics may have beneficial effects on patients with type 2 diabetes mellitus (T2DM). We separated 4 lactobacillus and 1 saccharomycetes from traditional fermented cheese whey (TFCW) and prepared composite probiotics from camel milk (CPCM) and investigated their effects on glucose and lipid metabolism, liver and renal function and gut microbiota in db/db mice.

Methods: CPCM was prepared in the laboratory and 40 db/db mice were randomly divided into 4 groups as metformin, low-dose and high-dose group and model group, and treated for 6 weeks. In addition, 10 C57BL/Ks mice as normal control group were used for comparison. Fasting blood glucose (FBG), body weight (BW), oral glucose tolerance test (OGTT), glycated hemoglobin (HbAlc), C-peptide (CP), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), 24 h urinary microalbumin (24 h malb), urine ketone, urine sugar, pancreas and liver tissue and intestinal flora were tested.

Results: Compared to diabetic group, high dose CPCM significantly decreased FBG, OGTT, HbAlc and IRI, plasma TC, TG, LDL-C, 24 h malb, urine ketone and urine sugar, increased CP, HDL-C levels, improved the liver and kidney function, protected the function of islets, also increased intestinal tract lactic acid bacteria and Bifidobacterium, decreased Escherichia in db/db mice.

Conclusion: CPCM decreased FBG, OGTT and HbAlc, increased CP, modulated lipid metabolism and improved liver and kidney protected injury in db/db mice, which may be related to various probiotics acting through protecting the function of islets and regulating intestinal flora disturbance.
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http://dx.doi.org/10.1186/s12906-021-03303-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061000PMC
April 2021

Efficacy and safety of ultrasound-guided radiofrequency ablation for low-risk papillary thyroid microcarcinoma in patients aged 55 years or older: a retrospective study.

Int J Hyperthermia 2021 ;38(1):604-610

School of Medicine, Nankai University, Tianjin, China.

Purpose: To evaluate the efficacy and safety of radiofrequency ablation for low-risk papillary thyroid microcarcinoma (PTMC) in patients aged 55 years or older.

Methods: This retrospective study included 95 patients aged 55 years or older who underwent radiofrequency ablation (RFA) for PTMCs between June 2014 and January 2019. Incidence and duration of postoperative complications were recorded and evaluated. Tumor volume and volume reduction rate (VRR) changes were calculated. Patients were also closely monitored for tumor recurrence, regrowth, and lymph node metastasis.

Results: All nodules were completely ablated. The mean initial volume of the ablated thyroid nodules was 107.27 ± 99.10 mm, and the volume decreased significantly during the follow-up time. The VRR in 1st, 3rd, 6th, 12th, 18th, 24th and 36th month were -591.64 ± 623.65%, -170.89 ± 319.51%, 9.74 ± 128.43%, 77.99 ± 45.26%, 99.35 ± 3.61%, 99.45 ± 3.05% and 99.78 ± 1.54%, respectively. No patient had any life-threatening complications. One patient had lymph node metastasis and one had a recurrence; both underwent a second radiofrequency ablation treatment and achieved satisfactory treatment results.

Conclusions: Our study suggests that radiofrequency ablation is a safe and effective option for low-risk PTMC in patients aged 55 years or older who are at a high risk of general anesthesia and postoperative complications or those who refuse surgery.
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http://dx.doi.org/10.1080/02656736.2021.1912416DOI Listing
January 2021

Semisynthesis of CRV431.

Org Lett 2021 May 12;23(9):3421-3425. Epub 2021 Apr 12.

State Key Laboratory of Chemical Oncogenomics and Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

The non-immune-suppressive cyclophilin inhibitor CRV431 is a clinical candidate to cure nonalcoholic steatohepatitis (NASH) and has the potential to treat liver fibrosis and cancer incidence. Herein we report a concise chemical semisynthesis of CRV431 in four steps from the commercially available cyclosporine, featuring in this the flow-chemistry-based methylenation an intermolecular ring-closing metathesis and a Rh-catalyzed diastereoselective hydrogenation.
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http://dx.doi.org/10.1021/acs.orglett.1c00881DOI Listing
May 2021

Tuning the reactivity of alkoxyl radicals from 1,5-hydrogen atom transfer to 1,2-silyl transfer.

Nat Commun 2021 Apr 9;12(1):2131. Epub 2021 Apr 9.

The Institute for Advanced Studies, Engineering Research Center of Organosilicon Compounds and Materials, Ministry of Education, Wuhan University, Wuhan, People's Republic of China.

Controlling the reactivity of reactive intermediates is essential to achieve selective transformations. Due to the facile 1,5-hydrogen atom transfer (HAT), alkoxyl radicals have been proven to be important synthetic intermediates for the δ-functionalization of alcohols. Herein, we disclose a strategy to inhibit 1,5-HAT by introducing a silyl group into the α-position of alkoxyl radicals. The efficient radical 1,2-silyl transfer (SiT) allows us to make various α-functionalized products from alcohol substrates. Compared with the direct generation of α-carbon radicals from oxidation of α-C-H bond of alcohols, the 1,2-SiT strategy distinguishes itself by the generation of alkoxyl radicals, the tolerance of many functional groups, such as intramolecular hydroxyl groups and C-H bonds next to oxygen atoms, and the use of silyl alcohols as limiting reagents.
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http://dx.doi.org/10.1038/s41467-021-22382-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035221PMC
April 2021

T-B cell epitope peptides induce protective immunity against Mycoplasma pneumoniae respiratory tract infection in BALB/c mice.

Immunobiology 2021 Mar 24;226(3):152077. Epub 2021 Mar 24.

Institute of Pathogenic Biology, Hengyang Medical College, University of South China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China. Electronic address:

Mycoplasma pneumoniae is the most common pathogen of community-acquired pneumonia in humans. Due to its high rates of antibiotic resistance, vaccination has become the best method to control the dissemination of M. pneumoniae. The recombinant carboxyl terminus of the P1 (P1C) protein is an immunodominant antigen, but it has negative effects such as poor stability and lower purity. In the current study, T-B epitopes of the P1C protein were predicted according to bioinformatics analysis and assessed for efficacy in peptide vaccination. BALB/c mice were subcutaneously inoculated with the T-B epitope peptides four times and then infected with M. pneumoniae through the respiratory tract. The results showed that the T-B epitope peptides of the P1C protein (P1C, P1C, P1C and P1C) induced strong antigen-specific serum antibody responses and cellular immune responses with high levels of serum IgG, IgA antibodies and Th1-biased (IFN-γ and IL-2) cytokines. Immunization with T-B epitope peptides significantly reduced the M. pneumoniae burden and the degree of inflammation in the challenged mice. Furthermore, the levels of IFN-γ and TNF-α in the supernatants of lung homogenates were observably reduced compared to those in the PBS group. Overall, our findings demonstrate that T-B epitopes (P1C, P1C, P1C and P1C) play significant roles in the P1C protein and can be used to induce powerful humoral and cellular immune responses to provide significant protection against M. pneumoniae pulmonary infection, which provides new insight into the design of potential multiepitope vaccines to prevent host infection by M. pneumoniae.
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http://dx.doi.org/10.1016/j.imbio.2021.152077DOI Listing
March 2021

Metastasis of primary breast angiosarcoma to axillary and supraclavicular lymph nodes: a rare case diagnosed using imaging data.

J Int Med Res 2021 Mar;49(3):3000605211002337

Department of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Primary breast angiosarcoma (PBA) is a rare malignant tumor. PBA usually undergoes hematogenous metastasis; lymph node metastasis is very rare in such patients, and metastasis of PBA to the supraclavicular lymph nodes has not previously been reported. Here, we describe a rare case of PBA manifested by a diffuse enlargement of the left breast, with metastasis to the left axillary and bilateral supraclavicular lymph nodes. Contrast-enhanced ultrasound and positron emission tomography findings indicated a malignant lesion, whereas magnetic resonance imaging suggested a benign lesion. Core needle biopsy identified the lesion as a lymphangioma, and the histological characteristics suggested a high-grade angiosarcoma. Multimodal imaging and perfusion patterns obtained using various contrast agents can thus help to diagnose PBA.
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http://dx.doi.org/10.1177/03000605211002337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020107PMC
March 2021

Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate.

J Cell Physiol 2021 Mar 27. Epub 2021 Mar 27.

Department of Urology and Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

An abnormal urine composition is a key reason for kidney stone formation, but little is known about the roles of small metabolites in the urine during kidney stone formation. Here, we found urine glycine in patients with kidney calcium oxalate (CaOx) stone was significantly lower than that in healthy people via H NMR spectra detection, and investigated the role and underlying mechanism of glycine in the regulation of CaOx stone formation. Our results showed that glycine could significantly attenuate ethylene glycol-induced CaOx crystal depositions in rat kidney via decreasing urine oxalate and increasing urine citrate. Mechanism studies revealed that glycine could decrease urine oxalate through downregulating Slc26a6 expression, whereas increase urine citrate via inhibiting Nadc1 expression. Moreover, glycine decreased the protein expression of both Slc26a6 and Nadc1 via increasing the expression of miRNA-411-3p, which directly bound to the 3'-untranslated regions of Slc26a6 and Nadc1 messenger RNAs, in vitro and in vivo. Together, our results revealed a novel role of glycine in the regulation of kidney CaOx crystal formation and provided a potential target for the treatment of kidney CaOx stone.
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http://dx.doi.org/10.1002/jcp.30370DOI Listing
March 2021

Corrigendum: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS.

Front Oncol 2021 1;11:661782. Epub 2021 Mar 1.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.3389/fonc.2019.01545.].
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http://dx.doi.org/10.3389/fonc.2021.661782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957925PMC
March 2021

Theoretical Model for N-Heterocyclic Carbene-Catalyzed Desymmetrizing [4 + 1] and [4 + 2] Annulations of an Enal and Aryldialdehyde with 1,3-Cyclopentenedione.

Org Lett 2021 Apr 16;23(7):2421-2425. Epub 2021 Mar 16.

Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, Henan China.

N-Heterocyclic carbene (NHC) catalyzed desymmetrizing reactions of olefins have rapidly developed in recent years; however, the origins of the chemo- and stereoselectivities of these reactions remain poorly understood. Herein, we propose a mechanistic map for these reactions to predict how chemo- and stereoselectivities are controlled by different active intermediates (i.e., Breslow and acylazolium intermediates). Remarkably, our findings contradict a previous proposition that product structures are determined by a transformation between a pair of isomers.
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http://dx.doi.org/10.1021/acs.orglett.1c00270DOI Listing
April 2021

Desymmetrization of 1,3-Diones by Catalytic Enantioselective Condensation with Hydrazine.

J Am Chem Soc 2021 Mar 16;143(11):4179-4186. Epub 2021 Mar 16.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Republic of Singapore, 117543.

We present herein an unprecedented desymmetrization of 1,3-diones by enantioselective intermolecular condensation. Under the catalysis by a chiral phosphoric acid, a range of readily available 1,3-diones undergo reaction with hydrazines to produce cyclic and acyclic keto-hydrazones bearing an all-carbon quaternary center in high efficiency and enantioselectivity. These compounds are also highly versatile for the preparation of multifunctional building blocks and heterocycles in excellent stereoselectivity.
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http://dx.doi.org/10.1021/jacs.1c01366DOI Listing
March 2021

Phenotypic Heterogeneity of Post-lingual and/or Milder Hearing Loss for the Patients With the GJB2 c.235delC Homozygous Mutation.

Front Cell Dev Biol 2021 26;9:647240. Epub 2021 Feb 26.

College of Otolaryngology, Head and Neck Surgery, Chinese People's Liberation Army (PLA) Institute of Otolaryngology, Chinese People's Liberation Army (PLA) General Hospital, Beijing, China.

Objective: To report the phenotypic heterogeneity of c.235delC homozygotes associated with post-lingual and/or milder hearing loss, and explore the possible mechanism of these unconditional phenotypes.

Methods: Mutation screening of was performed on all ascertained members from Family 1006983 and three sporadic patients by polymerase chain reaction (PCR) amplification and Sanger sequencing. Next generation sequencing (NGS) was successively performed on some of the affected members and normal controls from Family 1006983 to explore additional possible genetic codes. Reverse transcriptase-quantitative PCR was conducted to test the expression of Connexin30.

Results: We identified a Chinese autosomal recessive hearing loss family with the c.235delC homozygous mutation, affected members from which had post-lingual moderate to profound hearing impairment, and three sporadic patients with post-lingual moderate hearing impairment, instead of congenital profound hearing loss. NGS showed no other particular variants. Overexpression of Connexin30 in some of these cases was verified.

Conclusion: Post-lingual and/or moderate hearing impairment phenotypes of c.235delC homozygotes are not the most common phenotype, revealing the heterogeneity of pathogenic mutations. To determine the possible mechanism that rescues part of the hearing or postpones onset age of these cases, more cases are required to confirm both Connexin30 overexpression and the existence of modifier genes.
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http://dx.doi.org/10.3389/fcell.2021.647240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953049PMC
February 2021

A case report of complete pathological remission after chemotherapy in a patient with primary renal squamous cell carcinoma.

Transl Androl Urol 2021 Feb;10(2):997-1005

Day Ward of Chemotherapy, Qingdao Central Hospital, Qingdao, China.

This is the first case report of the outcomes of systemic chemotherapy in a patient with locally advanced renal squamous cell carcinoma, a rare tumor, as well as the first next generation sequencing study of this rare tumor. The patient's main symptoms were fever and low back pain. Initial positron emission tomography and computed tomography (PET-CT) suggested a malignant renal tumor at onset, but biopsy confirmed renal squamous cell carcinoma. Next generation sequencing showed a low level of microsatellite instability (MSI-L), a high tumor mutational burden (TMB-H), a high neoantigen burden (TNB-H), and a strong loss of heterozygosity (LOH) for human leukocyte antigen (HLA), with 67 deleterious mutations. The patient achieved partial radiological remission after a cycle of systemic chemotherapy with albumin-bound paclitaxel combined with nedaplatin. After radical resection of the left renal tumor, postoperative pathology confirmed complete tumor remission and tumor-like xanthogranulomatous pyelonephritis. Conclusion: This renal squamous cell carcinoma patient responded to systemic chemotherapy with paclitaxel combined with platinum, providing a reference for the future treatment of similar cases. Pathology and gene sequencing indicated that renal squamous cell carcinoma occurred in a background of active inflammation and that the tumor evolved immune escape mechanisms such as loss of HLA heterozygosity, with gene repair defects and TMB-H.
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http://dx.doi.org/10.21037/tau-20-1483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947427PMC
February 2021

Linking AM-PAC Mobility and Daily Activity to the PROMIS Physical Function Metric.

Phys Ther 2021 Mar 4. Epub 2021 Mar 4.

Department of Physical Therapy and Athletic Training, University of Utah.

Objective: The purpose of this study was to link Activity Measure for Post-Acute Care (AM-PAC) Mobility and Daily Activity scales to the PROMIS Physical Function (PF) allowing for a common metric across scales.

Methods: Cross-sectional study of patients 18 years and older presenting to one of eight outpatient rehabilitation clinics in southwestern Pennsylvania. Patients completed one survey with questions from the AM-PAC Daily Activity and Mobility short forms, and the PROMIS PF item bank. Using item response theory, we performed two rounds of fixed-parameter calibration. In the first, we calibrated the AM-PAC Daily Activity and Mobility items with 27 fixed item parameters from the PROMIS PF. Second, we calibrated the AM-PAC Daily Activity items with 11 PROMIS Upper Extremity fixed item parameters. This process uses the construct of physical function and equates AM-PAC items on the same underlying measurement scale for the PROMIS PF.

Results: Both scales measured a wide range of functioning and demonstrated good calibration. Data were appropriate for a fixed-parameter item response theory-based crosswalk. AM-PAC Daily Activity and Mobility raw scores were mapped onto the PROMIS PF metric. AM-PAC Daily Activity scores were also mapped onto the PROMIS PF Upper Extremity metric.

Conclusion: Question items from the AM-PAC Daily Activity, AM-PAC Mobility, and PROMIS PF similarly measure the construct of physical function. This consistency allows for a crosswalk of AM-PAC scores onto the PROMIS PF metric.

Impact: Crosswalk tables developed in this study allow for converting scores from the AM-PAC Daily Activity and Mobility scales to the PROMIS PF metric. This will facilitate monitoring of longitudinal change in function over time and across settings.
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http://dx.doi.org/10.1093/ptj/pzab084DOI Listing
March 2021

Synthesis of Mitochondria-Anchored Nitroimidazoles with a Versatile NIR Fluorophore for Hypoxic Tumor-Targeting Imaging and Chemoradiotherapy.

J Med Chem 2021 Mar 10;64(6):3381-3391. Epub 2021 Mar 10.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University (Army Medical University), 30 Gaotanyan Street, Shapingba District, Chongqing 400038, People's Republic of China.

Nitroimidazoles are one of the most common radiosensitizers investigated to combat hypoxia-induced resistance to cancer radiotherapy. However, due to poor selectivity distinguishing cancer cells from normal cells, effective doses of radiosensitization are much closer to the doses of toxicity induced by nitroimidazoles, limiting their clinical application. In this work, a tumor-targeting near-infrared (NIR) cyanine dye (IR-808) was utilized as a targeting ligand and an NIR fluorophore tracer to chemically conjugate with different structures of hypoxia-affinic nitroimidazoles. One of the NIR fluorophore-conjugated nitroimidazoles (808-NM2) was identified to preferentially accumulate in hypoxic tumor cells, sensitively outline the tumor contour, and effectively inhibit tumor growth synergistically by chemotherapy and radiotherapy. More importantly, nitroimidazoles were successfully taken into cancer cell mitochondria via 808-NM2 conjugate to exert the synergistic effect of chemoradiotherapy. Regarding the important roles of mitochondria on cancer cell survival and metastasis under hypoxia, 808-NM2 may be hopeful to fight against hypoxic tumors.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02250DOI Listing
March 2021

Research and Fabrication of Broadband Ring Flextensional Underwater Transducer.

Sensors (Basel) 2021 Feb 23;21(4). Epub 2021 Feb 23.

Acoustic Science and Technology Laboratory, Harbin Engineering University, Harbin 150001, China.

At present, high-speed underwater acoustic communication requires underwater transducers with the characteristics of low frequency and broadband. The low-frequency transducers also are expected to be low-frequency directional for realization of point-to-point communication. In order to achieve the above targets, this paper proposes a new type of flextensional transducer which is constructed of double mosaic piezoelectric ceramic rings and spherical cap metal shells. The transducer realizes broadband transmission by means of the coupling between radial vibration of the piezoelectric rings and high-order flexural vibration of the spherical cap metal shells. The low-frequency directional transmission of the transducer is realized by using excitation signals with different amplitude and phase on two mosaic piezoelectric rings. The relationship between transmitting voltage response (TVR), resonance frequency and structural parameters of the transducer is analyzed by finite element software COMSOL. The broadband performance of the transducer is also optimized. On this basis, the low-frequency directivity of the transducer is further analyzed and the ratio of the excitation signals of the two piezoelectric rings is obtained. Finally, a prototype of the broadband ring flextensional underwater transducer is fabricated according to the results of simulation. The electroacoustic performance of the transducer is tested in an anechoic water tank. Experimental results show that the maximum TVR of the transducer is 147.2 dB and the operation bandwidth is 1.5-4 kHz, which means that the transducer has good low-frequency, broadband transmission capability. Meanwhile, cardioid directivity is obtained at 1.4 kHz and low-frequency directivity is realized.
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http://dx.doi.org/10.3390/s21041548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926317PMC
February 2021

The quality of life in papillary thyroid microcarcinoma patients undergoing lobectomy or total thyroidectomy: A cross-sectional study.

Cancer Med 2021 03 26;10(6):1989-2002. Epub 2021 Feb 26.

Department of Ultrasound, General Hospital of Chinese PLA, Beijing, China.

Objective: Papillary thyroid microcarcinoma (PTMC) has a good prognosis and a long survival time, surgery is the common treatment including total thyroidectomy (TT) and unilateral lobectomy (LT), but recent studies showed that TT does not show an advantage over LT for PTMC in preventing cancer recurrence and reducing mortality. Given this, the health-related quality of life (HRQoL) has become one of the important factors that physicians must consider when making treatment decisions. The aim of this study was to compare the HRQoL of patients between undergoing TT and LT.

Methods: From October 2019 to December 2019, 69 PTMC patients were enrolled in our study, including 34 in the LT group and 35 in the TT group, respectively. We used three questionnaires which included the 36-item short-form health survey (SF-36), thyroid cancer-specific quality of life (THYCA-QOL), and Fear of Progression Questionnaire-Short Form (FoP-Q-SF) for each patient to evaluate their scores of HRQoL.

Results: According to the SF-36, the scores of the domain for the role limitation due to physical problems, emotional problems, and social function (RP, RE, and SF) as well as Physical Component Summary (PCS) and Mental Component Summary (MCS) showed a significant negative linear association between the LT group and TT group: RP (coefficient [coef]: -33.953 [confidence interval (CI) -51.187 to -16.720], p < 0.001, RE (coef: -21.633 [CI -39.500 to -3.766], p = 0.018), SF (coef: -10.169 [CI -19.586 to -0.752], p = 0.035)and PCS (coef: -10.571 [CI -17.768 to -3.373], p = 0.005), MCS (coef: -10.694 [CI -19.465 to -1.923], p = 0.018). The THYCA-QOL showed that the scores of the TT group were higher than that of the LT group in the problem of scar (coef: 16.245 [CI 1.697 to 30.794], p = 0.029 according to the multivariate analysis), suggesting a higher level of complaint in the TT group. There was no statistically significant difference in the scores of FoP-Q-SF between the two groups.

Conclusions: In patients with PTMC, LT offers an advantage over TT in terms of HRQoL, which supports the role of LT as an alternative strategy to TT.
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http://dx.doi.org/10.1002/cam4.3747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957180PMC
March 2021

Evaluation of Ultrasound-Guided Radiofrequency Ablation as a Treatment Option for Papillary Thyroid Microcarcinoma in the Isthmus: A Retrospective Study.

Front Endocrinol (Lausanne) 2020 9;11:599471. Epub 2021 Feb 9.

Department of Ultrasound, First Medical Center of General Hospital of Chinese PLA, Beijing, China.

Background: About 3-9.2% of papillary thyroid carcinomas (PTC) are found in the isthmus, which has unique anatomic properties, making treatment more challenging. The aim of this study was to evaluate the treatment and undesirable effects of ultrasound-guided radiofrequency ablation (RFA) for PTC in the isthmus.

Methods: This retrospective case series study assessed 112 patients with single papillary thyroid microcarcinoma in the isthmus, pathologically diagnosed before RFA at the General Hospital of Chinese PLA in 2014-2018. Follow-up was performed by contrast-enhanced ultrasound (CEUS) and ultrasound examinations at 1, 3, and 6 months and every 6 months thereafter. The complete ablation (CAR), disappearance (DR), and volume reduction (VRR) rates of nodules, the incidence of complications, and the rate of lymph-node metastasis were recorded.

Results: The CAR of the tumors was 100%. During follow-up, the volume of coagulation necrosis gradually decreased. DRs at 1, 3, 6, 12, and 18 months after RFA were 0.8% (1/112), 10.7% (12/112), 51.7% (58/112), 91.0% (102/112), and 100% (112/112), respectively. The VRR evaluated by ultrasound and CEUS gradually increased. One recurrent case (0.8%) was found at 7 months after RFA. No complications, lymph node metastasis confirmed by ultrasound, and abnormal thyroid function were observed.

Conclusions: This retrospective study shows that RFA is beneficial for the treatment of PTMC in the isthmus.
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http://dx.doi.org/10.3389/fendo.2020.599471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900160PMC
February 2021

Discovery of a Positron Emission Tomography Radiotracer Selectively Targeting the BD1 Bromodomains of BET Proteins.

ACS Med Chem Lett 2021 Feb 8;12(2):282-287. Epub 2021 Jan 8.

Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, United States.

In this paper, we report the design, synthesis, and biological evaluation of the first selective bromodomain and extra-terminal domain (BET) BD1 bromodomains of the PET radiotracer [F]PB006. The standard compound PB006 showed high affinity and good selectivity toward BRD4 BD1 ( = 100 nM and 29-fold selectively for BD1 over BD2) in an binding assay. PET imaging experiments in rodents were performed to evaluate the bioactivity of [F]PB006 . A biodistribution study of [F]PB006 in mice revealed high radiotracer uptake in peripheral tissues, such as liver and kidney, and moderate radiotracer uptake in the brain. Further blocking studies demonstrated the significant radioactivity decreasing (20-30% reduction compared with baseline) by pretreating unlabeled PB006 and JQ1, suggesting the high binding selectivity and specificity of [F]PB006. Our study indicated that [F]PB006 is a potent PET probe selectively targeting BET BD1, and further structural optimization of the radiotracer is still required to improve brain uptake to support neuroepigenetic imaging.
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http://dx.doi.org/10.1021/acsmedchemlett.0c00650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883468PMC
February 2021

How Solvents Control the Chemoselectivity in Rh-Catalyzed Defluorinated [4 + 1] Annulation.

Org Lett 2021 Feb 10;23(4):1489-1494. Epub 2021 Feb 10.

School of Chemistry and Chemical Engineering, and Chongqing Key Laboratory of Theoretical and Computational Chemistry, Chongqing University, Chongqing 400030, China.

Density functional theory calculations have been performed to reveal the chemoselectivity of Rh-catalyzed chiral C-F cleavage and γ-site functionalization. We found that the chemoselectivity is controlled by β-F elimination in methanol solvent, leading to formation of the alkynylic product. In isobutyronitrile solvent, the chemoselectivity is controlled by the allene insertion step, where the fluoroalkenylic product can be observed. The difference can be explained by analysis of the explicit solvent models.
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http://dx.doi.org/10.1021/acs.orglett.1c00223DOI Listing
February 2021

Degradation of 1,4-Dioxane by Xanthobacter sp. YN2.

Curr Microbiol 2021 Mar 6;78(3):992-1005. Epub 2021 Feb 6.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, China.

1,4-Dioxane is a highly toxic and carcinogenic pollutant found worldwide in groundwater and soil environments. Several microorganisms have been isolated by their ability to grow on 1,4-dioxane; however, low 1,4-dioxane tolerance and slow degradation kinetics remain obstacles for their use in 1,4-dioxane bioremediation. We report here the isolation and characterization of a new strain, Xanthobacter sp. YN2, capable of highly efficient 1,4-dioxane degradation. High degradation efficiency and high tolerance to 1,4-dioxane make this new strain an ideal candidate for the biodegradation of 1,4-dioxane in various treatment facilities. The maximum degradation rate of 1,4-dioxane was found to be 1.10 mg-1,4-dioxane/h mg-protein. Furthermore, Xanthobacter sp. YN2 was shown to grow in the presence of higher than 3000 mg/L 1,4-dioxane with little to no degradation inhibition. In addition, Xanthobacter sp. YN2 could grow on and degrade 1,4-dioxane at pH ranges 5 to 8 and temperatures between 20 and 40 °C. Xanthobacter sp. YN2 was also found to be able to grow on a variety of other substrates including several analogs of 1,4-dioxane. Genome sequence analyses revealed the presence of two soluble di-iron monooxygenase (SDIMO) gene clusters, and regulation studies determined that all of the genes in these two clusters were upregulated in the presence of 1,4-dioxane. This study provides insights into the bacterial stress response and the highly efficient biodegradation of 1,4-dioxane as well as the identification of a novel Group-2 SDIMO.
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http://dx.doi.org/10.1007/s00284-021-02347-6DOI Listing
March 2021