Publications by authors named "Yu Chen"

5,241 Publications

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Long Noncoding RNA LINC00899/miR-944/ESR1 Axis Regulates Cervical Cancer Cell Proliferation, Migration, and Invasion.

J Interferon Cytokine Res 2021 Jun;41(6):220-233

Department of Gynecology, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi, China.

Cervical cancer (CC) is a common malignancy in women. Long noncoding RNA LINC00899 plays a role in cancer, but its effects in CC are unknown. Our experiment aims to investigate the specific effects of LINC00899 in CC. LINC00899 was lowly expressed in CC tissues and cells, and overexpressed LINC0089 inhibited the viability, proliferation, migration, and invasion of CC cells, whereas silencing of LINC00899 had the opposite effect. There is a targeting relationship between LINC0089 and miR-944. It was found that miR-944 could competitively bind with LINC00899, and LINC00899 in turn, could downregulate expression of miR-944. Moreover, estrogen receptor 1 (ESR1) was the target gene of miR-944. Overexpressed miR-944 inhibited ESR1 expression, yet enhanced the migration and invasion of CC cells and promoted the expression levels of N-cadherin and Vimentin while inhibiting the expression of E-cadherin. However, overexpressed ESR1 reversed the effect of miR-944 overexpression on CC cells. LINC00899/miR-944/ESR1 axis regulates the proliferation, migration, and invasion of CC cells by regulating the expression levels of related proteins.
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http://dx.doi.org/10.1089/jir.2021.0023DOI Listing
June 2021

Accuracy of stroke volume variation and pulse pressure variation to predict fluid responsiveness in patients with thoracic kyphosis.

Ann Palliat Med 2021 Jun 21. Epub 2021 Jun 21.

Department of Anesthesiology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, China.

Background: This study aims to evaluate the ability of stroke volume variation (SVV) and pulse pressure variation (PPV) to predict fluid responsiveness in mechanically ventilated patients with thoracic kyphosis.

Methods: A total of 35 patients diagnosed with thoracic kyphosis undergoing corrective surgery were studied. For all patients, the Vigileo/FloTrac system was used for analysis. Hemodynamic data such as mean arterial pressure (MAP), heart rate (HR), stroke volume (SV), stroke volume index (SVI), cardiac output (CO), cardiac output index (CI), SVV, and PPV were recorded before and after volume expansion (VE). Fluid responsiveness was defined as an increase in SVI ≥10% (ΔSVI ≥10%). Patients were divided into responders and non-responders as determined by changes in ΔSVI ≥10% and <10%. Nonparametric Wilcoxon rank sum test was used to compare the hemodynamic parameters of Responders and Non-responders before and after VE. Pearson correlation analysis was used to analyze the values of SVV, PPV and ΔSVI. The receiver operating characteristic (ROC) curve of each hemodynamic index was drawn to determine its accuracy and threshold.

Results: Two patients were excluded. There was no significant difference in patients' characteristics between Responders and Non-responders. After VE, there were no significant changes in HR, MAP, and SV in both responders and non-responders, but CI were significantly changed in the two groups. SVI and CO increased significantly in responders before and after VE, but not in non-responders. VE also caused decreases of PPV and SVV in both responders and non-responders. Before VE, the SVV and PPV correlated with ΔSVI in responders (r=0.621, r=0.569, respectively, P<0.05), but neither the SVV nor PPV correlated with ΔSVI in non-responders (P>0.05). The areas under the ROC curves of patients with thoracic kyphosis were 0.872 (95% CI: 0.719-1.000) for SVV and 0.833 (95% CI: 0.667-1.000) for PPV. The threshold of the SVV of patients with thoracic kyphosis was 13.5%, and the threshold of PPV was 14.5%.

Conclusions: Both SVV and PPV can be used as effective indictors to monitor volume changes in patients with thoracic kyphosis.
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http://dx.doi.org/10.21037/apm-21-1211DOI Listing
June 2021

Differential microRNA Expression in Newcastle Disease Virus-Infected HeLa Cells and Its Role in Regulating Virus Replication.

Front Oncol 2021 4;11:616809. Epub 2021 Jun 4.

Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

As an oncolytic virus, Newcastle disease virus (NDV) can specifically kill tumor cells and has been tested as an attractive oncolytic agent for cancer virotherapy. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which can greatly influence viral replication and pathogenesis. However, the interplay between NDV replication and cellular miRNA expression in tumor cells is still largely unknown. In the present study, we compared the profiles of cellular miRNAs in uninfected and NDV-infected HeLa cells by small RNA deep sequencing. Here we report that NDV infection in HeLa cells significantly changed the levels of 40 miRNAs at 6 h post-infection (hpi) and 62 miRNAs at 12 hpi. Among 23 highly differentially expressed miRNAs, NDV infection greatly promoted the levels of 3 miRNAs and suppressed the levels of 20 miRNAs at both time points. These 23 miRNAs are predicted to target various genes involved in virus replication and antiviral immunity such as ErbB, Jak-STAT, NF-kB and RIG-I-like receptor. Verification of deep sequencing results by quantitative RT-PCR showed that 9 out of 10 randomly selected miRNAs chosen from this 23-miRNA pool were consistent with deep sequencing data, including 6 down-regulated and 3 up-regulated. Further functional research revealed that hsa-miR-4521, a constituent in this 23-miRNA pool, inhibited NDV replication in HeLa cells. Moreover, dual-luciferase and gene expression array uncovered that the member A of family with sequence similarity 129 (FAM129A) was directly targeted by hsa-miR-4521 and positively regulated NDV replication in HeLa cells, indicating that hsa-miR-4521 may regulate NDV replication interaction with FAM129A. To our knowledge, this is the first report of the dynamic cellular miRNA expression profile in tumor cells after NDV infection and may provide a valuable basis for further investigation on the roles of miRNAs in NDV-mediated oncolysis.
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http://dx.doi.org/10.3389/fonc.2021.616809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211993PMC
June 2021

Stem cell transplantation for treating liver diseases: progress and remaining challenges.

Am J Transl Res 2021 15;13(5):3954-3966. Epub 2021 May 15.

The Fourth Liver Disease Center, Beijing Youan Hospital, Capital Medical University Beijing 100069, China.

With the development of regenerative medicine, various stem cells are increasingly considered for treating liver diseases. Various stem cells have been reported to play an essential role in liver recovery, and studies have verified the preliminary effectiveness and safety of these therapies. Stem cell-based therapies will emerge as an effective treatment strategy for liver diseases. Thus, the research progress and challenges to the related stem cells were reviewed, namely the classification of stem cells, cell culture, transplantation, cell tracing in the body, therapies for various liver diseases.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205777PMC
May 2021

LINC00665/miR-379-5p/GRP78 regulates cisplatin sensitivity in gastric cancer by modulating endoplasmic reticulum stress.

Cytotechnology 2021 Jun 26;73(3):413-422. Epub 2021 Apr 26.

Department of Thoracic Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210029 Jiangsu Province China.

Acquired resistance to cisplatin (DDP)-based chemotherapy greatly hinders the treatment of gastric cancer (GC). LINC00665 serves as an oncogene in GC. Hence, the current study was designed to investigate the regulatory effects of LINC00665 on DDP-resistance of GC. LINC00665 and miR-379-5p expression levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Glucose regulated protein 78 (GRP78) protein level was measured by western blot assay. Interactions between LINC00665 and miR-379-5p or between miR-379-5p and GRP78 were verified by dual luciferase reporter assay. Cell counting kit 8 (CCK-8) assay and flow cytometry assay respectively determine the proliferative ability and apoptosis of GC cells. Western blot analysis was also performed to detect the protein levels of C/EBP-homologous protein (CHOP), X box binding protein (XBP1) and apoptosis-related proteins. In addition, GRP78 expression was evaluated by immunofluorescence. It was observed that the expression levels of LINC00665 and GRP78 were upregulated, and the expression level of miR-379-5p was downregulated in DDP-sensitive and DDP-resistant GC cell lines. What's more, GRP78 expression and the cell growth inhibition rates of DDP-sensitive and DDP-resistant GC cells had a negative correlation. Additionally, miR-379-5p was a target miRNA of LINC00665, and GRP78 was a target mRNA of miR-379-5p. Functional studies revealed that knockdown of LINC00665 inhibited DDP-resistant GC cell proliferation, induced apoptosis as well as suppressed Endoplasmic reticulum (ER) stress. Mechanistically, knockdown of LINC00665 downregulated GRP78 expression by strengthening miR-379-5p. LINC00665 silencing could overcome DPP-resistance of GC cells by downregulating GRP78 via sponging miR-379-5p, indicating that LINC00665 might be a potential therapeutic target for DDP- resistant GC patients.
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http://dx.doi.org/10.1007/s10616-021-00466-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167010PMC
June 2021

Facile Synthesis of FePS [email protected] Composite as a High-Performance Anode Material for Sodium Storage.

Nanomicro Lett 2020 Feb 18;12(1):54. Epub 2020 Feb 18.

Jiangsu Key Laboratory of Materials and Technology for Energy Conversion, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, People's Republic of China.

Searching for advanced anode materials with excellent electrochemical properties in sodium-ion battery is essential and imperative for next-generation energy storage system to solve the energy shortage problem. In this work, two-dimensional (2D) ultrathin FePS nanosheets, a typical ternary metal phosphosulfide, are first prepared by ultrasonic exfoliation. The novel 2D/2D heterojunction of FePS [email protected] composite is then successfully synthesized by in situ mixing ultrathin MXene nanosheets with FePS nanosheets. The resultant FePS [email protected] hybrids can increase the electronic conductivity and specific surface area, assuring excellent surface and interfacial charge transfer abilities. Furthermore, the unique heterojunction endows FePS [email protected] composite to promote the diffusion of Na and alleviate the drastic change in volume in the cyclic process, enhancing the sodium storage capability. Consequently, the few-layered FePS nanosheets uniformly coated by ultrathin MXene provide an exceptional reversible capacity of 676.1 mAh g at the current of 100 mA g after 90 cycles, which is equivalent to around 90.6% of the second-cycle capacity (746.4 mAh g). This work provides an original protocol for constructing 2D/2D material and demonstrates the [email protected] composite as a potential anode material with excellent property for sodium-ion batteries.
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http://dx.doi.org/10.1007/s40820-020-0381-yDOI Listing
February 2020

Simvastatin increases circulating endothelial progenitor cells and inhibits the formation of intracranial aneurysms in rats with diet-induced hyperhomocysteinemia.

Neurosci Lett 2021 Jun 17:136072. Epub 2021 Jun 17.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Background And Purpose: Endothelial dysfunction triggers early pathological changes in artery, leading to the formation of intracranial aneurysm (ICA). Increase in plasma homocysteine (Hcy) impairs endothelium and endothelial progenitor cells (EPCs) are critical in repairing damaged endothelium. The aim of this study was to assess the impact of simvastatin on ICA formation in rats with hyperhomocysteinemia (HHcy).

Methods: ICAs were induced in Male Sprague-Dawley rats after surgical induction in the presence of HHcy induced by a high L-methionine diet with or without oral simvastatin treatment. The size and media thickness of ICAs were evaluated 2 months after aneurysm induction. EPCs and serum vascular endothelial grow factor (VEGF) were measured be flow cytometry and ELISA respectively. Plasma Hcy levels and expression of VEGF, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-2 (MMP-2), and MMP-9 in aneurysmal walls were examined and correlated with ICA formation.

Results: HHcy accelerates ICA formation and rats treated with simvastatin exhibited a significant increase in media thickness and a reduction in aneurysmal size. Simvastatin increased levels of circulating EPCs and decreased iNOS, MMP-2, MMP-9 and VEGF mRNA levels, while increased eNOS mRNA in aneurysmal tissue.

Conclusion: In a rat model, HHcy reduces circulating EPCs and accelerates ICA formation. Simvastatin treatment increases circulating EPCs and inhabits the formation of ICA. We have shown a close association among circulating EPCs, biochemical markers related to vascular remodeling and the formation of ICA.
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http://dx.doi.org/10.1016/j.neulet.2021.136072DOI Listing
June 2021

Engineering Single-Atomic Iron-Catalyst-Integrated 3D-Printed Bioscaffolds for Osteosarcoma Destruction with Antibacterial and Bone Defect Regeneration Bioactivity.

Adv Mater 2021 Jun 19:e2100150. Epub 2021 Jun 19.

Department of Medical Ultrasound, Shanghai Tenth People's Hospital, Ultrasound Research and Education Institute, Tongji University School of Medicine, Tongji University Cancer Center, Shanghai Engineering Research Center of Ultrasound Diagnosis and Treatment, National Clinical Research Center of Interventional Medicine, Shanghai, 200072, P. R. China.

Effective antitumor therapeutics with distinctive bactericidal and osteogenic properties are in high demand for comprehensive osteosarcoma treatment. Here, a "scaffold engineering" strategy that integrates highly active single-atomic iron catalysts (FeSAC) into a 3D printed bioactive glass (BG) scaffold is reported. Based on the atomically dispersed iron species within the catalysts, the engineered FeSAC displays prominent Fenton catalytic activity to generate toxic hydroxyl radicals (•OH) in response to the microenvironment specific to osteosarcoma. In addition, the constructed FeSAC-BG scaffold can serve as a sophisticated biomaterial platform for efficient osteosarcoma ablation, with concomitant bacterial sterilization via localized hyperthermia-reinforced nanocatalytic therapeutics. The destruction of the osteosarcoma, as well as the bacterial foci, can be achieved, further preventing susceptible chronic osteomyelitis during osteogenesis. In particular, the engineered FeSAC-BG scaffold is identified with advances in accelerated osteoconduction and osteoinduction, ultimately contributing to the sophisticated therapeutics and management of osteosarcoma. This work broadens the biomedical potential of single-atom catalysts and offers a comprehensive clinically feasible strategy for overall osteosarcoma therapeutics, bacterial inhibition, and tissue regeneration.
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http://dx.doi.org/10.1002/adma.202100150DOI Listing
June 2021

Ether-linked porphyrin covalent organic framework with broadband optical switch.

iScience 2021 Jun 11;24(6):102526. Epub 2021 May 11.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.

It is still a challenge to design and synthesize novel switchable optical materials with ultrafast nonlinear optical (NLO) response in a broad spectral range. These materials have exhibited great application potential in many high-technology fields such as biological imaging, chemical sensors, optical data storage, laser protection, and controllable intelligent and optoelectronic devices. By using porphyrins with highly delocalized 18 π-electron conjugated system as functional building blocks, the first ether-linked porphyrin covalent organic framework materials (COF-Pors) with highly ordered lattice structure have been successfully synthesized. In contrast to the starting porphyrins that only exhibit reverse saturable absorption (RSA) response at 532 nm, the as-prepared COF-Pors shows large NLO effect in a broad range from visible to near infrared. Upon laser illumination, COF-Pors exhibits typical saturable absorption (SA) effect at lower incident laser energy, and RSA response at higher pulse energy.
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http://dx.doi.org/10.1016/j.isci.2021.102526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188477PMC
June 2021

Pathogenicity of novel reassortant Eurasian avian-like H1N1 influenza virus in pigs.

Virology 2021 Jun 6;561:28-35. Epub 2021 Jun 6.

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China. Electronic address:

Reassortant Eurasian avian-like (EA) H1N1 virus, possessing 2009 pandemic (pdm/09) and triple-reassortant (TR)-derived internal genes, namely G4 genotype, has replaced the G1 genotype EA H1N1 virus (all the genes were of EA origin) and become predominant in swine populations in China. Understanding the pathogenicity of G4 viruses in pigs is of great importance for disease control. Here, we conducted comprehensive analyses of replication and pathogenicity of G4 and G1 EA H1N1 viruses in pigs. G4 virus exhibited enhanced replication, increased duration of virus shedding, and caused more severe respiratory lesions in pigs compared with G1 virus. G4 virus, with viral ribonucleoprotein (vRNP) complex genes of pdm/09 origin, exhibited higher levels of nuclear accumulation and higher polymerase activity, which is essential for improved replication of G4 virus. These findings indicate that G4 virus poses a great threat to both swine industry and public health, and control measures should be urgently implemented.
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http://dx.doi.org/10.1016/j.virol.2021.06.001DOI Listing
June 2021

MiR-193a-3p targets LGR4 to promote the inflammatory response in endometritis.

Int Immunopharmacol 2021 Jun 15;98:107718. Epub 2021 Jun 15.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

Solving the reproductive barriers of dairy cows has become one of the most critical factors determining the dairy industry's development. Clinically, inflammation disease like endometritis is the most crucial cause in reducing dairy production's financial viability. MiR-193 family can induce cell apoptosis and differentiation has been reported in various diseases. LGR4 plays a vital role in reproductive system development and immune system regulation, and it is closely related to animal reproductive function and cytokine regulation. In this study, we observed a negative relationship between miR-193a-3p and LGR4 expression level in both inflammatory tissues and cells. The expression level of miR-193a-3p and LGR4 in bovine endometrial epithelial cells (BENDs) is regulated by lipopolysaccharide (LPS) stimulation time and dose-dependent. Subsequently, miR-193a-3p mimics and inhibitors were used to explore its functions in the inflammation response process, finding that overexpression of miR-193a-3p markedly increases the expression level of pro-inflammatory cytokines induced by LPS, such as IL-1β, IL-6 and TNF-α, while the group in which transfected inhibitor is on the contrary. Of note, immunofluorescence and western blot results showed that miR-193a-3p enhanced LPS-induced NF-κB p65 phosphorylation through targeting LGR4, whereas inhibiting miR-193a-3p could suppress the activation of NF-κB pathway significantly. In conclusion, our study firstly reported the mechanism by which miR-193a-3p targets LGR4 to elevate the inflammatory response in bovine endometrium injury, thereby implying that knockdown miR-193a-3p may lay the theoretical and practical basis for drug development of alleviating endometritis in dairy cows.
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http://dx.doi.org/10.1016/j.intimp.2021.107718DOI Listing
June 2021

Prevalence of bovine tuberculosis in dairy cattle in China during 2010-2019: A systematic review and meta-analysis.

PLoS Negl Trop Dis 2021 Jun 17;15(6):e0009502. Epub 2021 Jun 17.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, PR China.

Background: Bovine tuberculosis (bTB), caused by members of the Mycobacterium tuberculosis complex bacteria, mainly Mycobacterium bovis (M. bovis), is a major threat to public health and economic development. There has been no systematic epidemiological assessment concerning bTB in dairy cattle in China.

Methodology/principal Findings: Literature related to bTB in China was retrieved from China National Knowledge Infrastructure (CNKI), PubMed, ScienceDirect, VIP Chinese Journals Database, and Wan Fang Database to build the first meta-analysis for estimating the prevalence and infection moderators of bTB in dairy cattle in China. A total of 100 relevant studies published from 2010 to 2019 were included. We estimated the overall prevalence of bTB was 2.4% (95% CI: 2.1-2.8) during this decade. In the sampling year subgroup, the prevalence was lowest in 2017 or later at 0.8% (95% CI: 0.3-1.5). The lowest prevalence was 0.7% (95% CI: 0.5-1.0) in Northwestern China. The lowest prevalence was 2.1% (95% CI: 1.8-2.5) using SIT test. Heifer cows had the highest prevalence, which was 27.1% (95% CI: 9.7-49.2). The prevalence in scale farming was 3.7% (95% CI: 3.1-4.3), significantly higher than that in free-range farming (1.7%, 95% CI: 1.1-2.4). The prevalence of bTB was highest in summer at 4.0% (95% CI: 1.7-7.0). In addition, the influence of different geographical factors (altitude, longitude, latitude, precipitation, temperature, humidity) on the prevalence was analyzed.

Conclusions/significance: The results showed that bTB was widespread in China but has been gradually reduced through concerted national intervention. It is suggested that different countries should formulate corresponding prevention and control measures according to the epidemic situation in its cattle industry. Enhanced monitoring of warm and humid areas may play an important role in reducing the incidence of bTB. In addition, when large-scale breeding is promoted, attention should be paid to standardizing breeding management and improving animal welfare to reduce the prevalence of bTB in cattle.
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http://dx.doi.org/10.1371/journal.pntd.0009502DOI Listing
June 2021

NbC MXene-Functionalized Scaffolds Enables Osteosarcoma Phototherapy and Angiogenesis/Osteogenesis of Bone Defects.

Nanomicro Lett 2021 Jan 4;13(1):30. Epub 2021 Jan 4.

School of Life Sciences, Shanghai University, Shanghai, 200444, People's Republic of China.

Early surgical resection and chemotherapy of bone cancer are commonly used in the treatment of bone tumor, but it is still highly challenging to prevent recurrence and fill the bone defect caused by the resection site. In this work, we report a rational integration of photonic-responsive two-dimensional (2D) ultrathin niobium carbide (NbC) MXene nanosheets (NSs) into the 3D-printed bone-mimetic scaffolds (NBGS) for osteosarcoma treatment. The integrated 2D NbC-MXene NSs feature specific photonic response in the second near-infrared (NIR-II) biowindow with high tissue-penetrating depth, making it highly efficient in killing bone cancer cells. Importantly, Nb-based species released by the biodegradation of NbC MXene can obviously promote the neogenesis and migration of blood vessels in the defect site, which can transport more oxygen, vitamins and energy around the bone defect for the reparative process, and gather more immune cells around the defect site to accelerate the degradation of NBGS. The degradation of NBGS provides sufficient space for the bone remodeling. Besides, calcium and phosphate released during the degradation of the scaffold can promote the mineralization of new bone tissue. The intrinsic multifunctionality of killing bone tumor cell and promoting angiogenesis and bone regeneration makes the engineered NbC MXene-integrated composite scaffolds a distinctive implanting biomaterial on the efficient treatment of bone tumor.
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http://dx.doi.org/10.1007/s40820-020-00547-6DOI Listing
January 2021

Prognostic Value of Elevated Cardiac Troponin I After Aneurysmal Subarachnoid Hemorrhage.

Front Neurol 2021 31;12:677961. Epub 2021 May 31.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Patients with aneurysmal subarachnoid hemorrhage (aSAH) have an increased incidence of cardiac events and short-term unfavorable neurological outcomes during the acute phase of bleeding. We studied whether troponin I elevation after ictus can predict future major adverse cardiac events (MACEs) and long-term neurological outcomes after 2 years. Consecutive aSAH patients within 3 days of bleeding were eligible for review from a prospective observational cohort (ClinicalTrials.gov Identifier: NCT04785976). Potential predictors of future MACEs and unfavorable long-term neurological outcomes were calculated by Cox and logistic regression analyses. Additional Kaplan-Meier curves were performed. A total of 213 patients were enrolled with an average follow-up duration of 34.3 months. Individuals were divided into two groups: elevated cTnI group and unelevated cTnI group. By the last available follow-up, 20 patients had died, with an overall all-cause mortality rate of 9.4% and an annual all-cause mortality rate of 3.8%. Patients with elevated cTnI had a significantly higher risk of future MACEs (10.6 vs. 2.1%, = 0.024, and 95% CI: 1.256-23.875) and unfavorable neurological outcomes at discharge, 3-month, 1-, 2-years, and last follow-up ( = 0.001, < 0.001, = 0.001, < 0.001, and < 0.001, respectively). In the Cox analysis for future MACE, elevated cTnI was the only independent predictor (HR = 5.980; 95% CI: 1.428-25.407, and = 0.014). In the multivariable logistic analysis for unfavorable neurological outcomes, peak cTnI was significant (OR = 2.951; 95% CI: 1.376-6.323; = 0.005). Kaplan-Meier analysis indicated that the elevated cTnI was correlated with future MACE (log-rank test, = 0.007) and subsequent death (log-rank test, = 0.004). cTnI elevation after aSAH could predict future MACEs and unfavorable neurological outcomes.
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http://dx.doi.org/10.3389/fneur.2021.677961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200557PMC
May 2021

Prognostic Factors Related to the Mortality Rate of Acute-on-Chronic Liver Failure Patients.

Diabetes Metab Syndr Obes 2021 9;14:2573-2580. Epub 2021 Jun 9.

Difficult & Complicated Liver diseases and Artificial Liver Center, Beijing Youan Hospital, Capital Medical University, Beijing, People's Republic of China.

Background: Critical illness-related corticosteroid insufficiency (CIRCI) is known to be a common complication in patients with acute-on-chronic liver failure (ACLF). However, factors that predict the survival rate of ACLF patients remain unclear. The present study aims to determine the prognostic factors that impinge on the survival rate of ACLF patients.

Methods: A total of 90 patients with ACLF at different stages, with or without CIRCI, were prospectively evaluated.

Results: Various clinical factors were found to be significantly different among patients at early, mid and late stages of ACLF, as well as between the same population of patients with and without CIRCI. Specifically, patients at later stages of ACLF and patients with CIRCI had significantly higher Child-Turcotte-Pugh (CTP), Model for End-Stage Liver Disease (MELD) and ACLF Research Consortium (AARC)-ACLF scores. CIRCI was observed in 20% of the enrolled patients (18 out of 90). In addition, the 90-day mortality rate was higher in mid- and late-stage ACLF patients, as well as patients with CIRCI.

Conclusion: ACLF stage and CIRCI predict early mortality in patients with ACLF and could be actively monitored in these patients for prioritized liver transplantation.
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http://dx.doi.org/10.2147/DMSO.S309641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200164PMC
June 2021

Cerebellar venous thrombosis mimicking a cerebellar tumor due to polycythemia vera: a case report.

BMC Neurol 2021 Jun 16;21(1):225. Epub 2021 Jun 16.

Department of Neurology, Aerospace Center Hospital, Beijing, China.

Background: Cerebral venous thrombosis (CVT) occurs rarely in the general population and is frequently associated with confused clinical findings and delayed diagnosis. Isolated cerebellar cortical vein thrombosis is a very rare phenomenon.

Case Presentation: This report describes a case with CVT, which is manifested as space-occupying lesions of the cerebellar hemisphere and mimics a cerebellar tumor at the beginning. The diagnosis of CVT was finalized given the laboratory and brain biopsy findings. The etiology may be related to polycythemia vera with Janus Kinase 2 V617F mutation.

Conclusion: Isolated cerebellar vein thrombosis should be considered when swelling and enhancing cerebellar lesions are detected. Polycythemia vera, especially with a positive JAK2 V617F mutation, may be a rare risk factor for CVT.
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http://dx.doi.org/10.1186/s12883-021-02261-1DOI Listing
June 2021

EXPRESS: Characteristics and Prognosis of Patients with Embolic Stroke of Undetermined Source in China.

Int J Stroke 2021 Jun 14:17474930211028040. Epub 2021 Jun 14.

Beijing Tiantan Hospital, Beijing, China.

Background And Purpose: We aimed to explore the frequencies, risk factors, and natural history of ESUS through a national prospective registry in China.

Methods: Between August 2015 and March 2018, the Third China National Stroke Registry (CNSR-III) recruited consecutive patients with ischemic stroke or transient ischemic attack (TIA) in China. The baseline characteristics, risks of stroke, and mortality in patients with ESUS were described and compared with that in patients with other causative subtypes.

Results: A total of 15166 TIA and ischemic stroke patients were enrolled in CNSR-III. Among 8528 ischemic stroke with standard diagnostic work-up, 2415 (28.3%) patients were diagnosed with ESUS. The mean age was 61 years and 70% of them were male. Compared to patients with cardioembolic strokes and small vessel disease, patients with ESUS had higher prevalence of nonstenosing large artery atherosclerosis (37.93% vs 31.26%, P=0.008 and 37.93% vs 34.40%, P=0.044 respectively). The cumulative probability of stroke recurrence in patients with ESUS at 3 month and 1 year was 5.59% and 8.74%. Compared with ESUS patients (0.70% and 1.99%), patients with the large artery atherosclerosis and cardioembolic strokes had higher cumulative probability of death at 3 month (1.94% and 3.22%) and 1 year (4.17% and 7.39%).

Conclusions: ESUS is a common cause of ischemic stroke in Chinese population with a higher stroke recurrence of ESUS than previous reported. It was more likely to have nonstenosing large artery atherosclerosis in patients with ESUS than with cardioembolic strokes and small vessel disease.
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http://dx.doi.org/10.1177/17474930211028040DOI Listing
June 2021

Lung Cancer Cell-Derived Exosomal let-7d-5p Down-Regulates OPRM1 to Promote Cancer-Induced Bone Pain.

Front Cell Dev Biol 2021 26;9:666857. Epub 2021 May 26.

Department of Pain Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Cancer-induced bone pain (CIBP) is the pain caused by metastasis of malignant tumors to the bone, accounting for more than half of all chronic cancer pain, which seriously affects the quality of life among tumor patients. Up to 40% of patients with advanced lung cancer suffer from CIBP. MicroRNA (miRNA) transfers between cells through exosomes, mediates cell-to-cell communication, and performs various biological functions. Studies have shown that miRNAs secreted by cancer can modify the tumor microenvironment, but whether exosome-mediated miRNA transfer plays a role in CIBP is still unknown. In this study, the expression levels of 15 miRNAs in exosomes derived A549 cells and 18 miRNAs in exosomes derived NCI-H1299 cells were significantly up-regulated, and qRT-PCR further confirmed that the level of let-7d-5p was increased most considerably. , exosomal let-7d-5p (EXO let-7d-5p) can be taken up by dorsal root ganglion (DRG) neurons and inhibit the protein level of the target gene opioid receptor mu 1 (OPRM1). EXO let-7d-5p was further confirmed to be involved in the generation and maintenance of CIBP . Our findings clarify the molecular mechanism of CIBP caused by the inhibition of OPRM1 by EXO let-7d-5p, providing new clues and intervention targets for the prevention and treatment of CIBP.
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http://dx.doi.org/10.3389/fcell.2021.666857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188355PMC
May 2021

Enteral lorlatinib after alectinib as a treatment option in anaplastic lymphoma kinase-positive non-small cell lung cancer with triple problems: carcinomatous meningitis, poor performance status, and dysphagia-a case report.

Respirol Case Rep 2021 Jul 3;9(7):e00796. Epub 2021 Jun 3.

Department of Medical Oncology Hirosaki University Graduate School of Medicine Aomori Japan.

Alectinib treatment is effective in patients with anaplastic lymphoma kinase gene rearrangement-positive non-small cell lung cancer (NSCLC; hereafter positive NSCLC) who exhibit central nervous system (CNS) relapse and poor performance status (PS). Lorlatinib treatment is effective upon failure of other ALK inhibitor-based treatments. However, much remains unknown about the efficacy of lorlatinib in patients with positive NSCLC, who have triple problems, carcinomatous meningitis, poor PS, and dysphagia, after alectinib treatment. Here, we report the remarkable response of a 73-year-old patient with positive NSCLC showing carcinomatous meningitis due to CNS metastases, poor PS, and dysphagia to lorlatinib. Lorlatinib administration through a nasogastric tube alleviated complications related to consciousness within three days, and the patient survived for 16 months after CNS relapse. Lorlatinib could be a treatment option for patients with positive NSCLC showing carcinomatous meningitis, poor PS, and dysphagia upon failure of other inhibitor-based treatments.
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http://dx.doi.org/10.1002/rcr2.796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173452PMC
July 2021

The Central Roles of Noncoding RNA in Estrogen-Dependent Female Reproductive System Tumors.

Int J Endocrinol 2021 24;2021:5572063. Epub 2021 May 24.

Department of Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

The pathogenesis of ovarian and endometrial cancers is closely associated with estrogen-related pathways. These estrogen-dependent tumors seriously threaten the health and quality of life in women. Noncoding RNAs (ncRNAs) are defined as RNAs that do not encode proteins, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), both of which have been reported in estrogen-dependent female reproductive system tumors. This review systematically summarizes the role of ncRNAs in estrogen-dependent tumors and common patterns of regulatory mechanisms to explore their future research directions in tumor diagnosis, treatment, and prognosis. This may provide new ideas for the potential application of ncRNAs in estrogen-dependent female reproductive system tumors.
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http://dx.doi.org/10.1155/2021/5572063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169271PMC
May 2021

Current and Future Roles of Circular RNAs in Normal and Pathological Endometrium.

Front Endocrinol (Lausanne) 2021 26;12:668073. Epub 2021 May 26.

Department of Gynecology, Shenzhen Second People's Hospital/The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

The uterine endometrium, which lines the mammalian uterus, is essential for embryo implantation. This lining undergoes significant changes during sexual and menstrual cycles. The endometrium is also associated with hormone-related diseases such as endometriosis and endometrial cancer. Circular RNAs (circRNAs) play a role in various biological processes. Recent studies have determined that circRNAs function in both normal and pathological endometrial environments. Here, we review high-throughput studies pertaining to circRNAs as well as individual circRNAs active in the endometrium, in order to explore the myriad functions of circRNAs in the endometrium and mechanisms underlying these functions, from panoramic and individual perspectives. Owing to their abundant expression, stability, and small size, circRNAs have displayed potential usefulness as diagnostic markers and treatment targets for endometrial-related diseases. Therefore, the specific role of circRNAs in the endometrium warrants systematic investigation in the future.
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http://dx.doi.org/10.3389/fendo.2021.668073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187767PMC
May 2021

Correlation of exercise style to mental health and social adaptability of college students.

Authors:
Yu Chen Chloe Sto

Work 2021 Jun 6. Epub 2021 Jun 6.

Department of Radioisotope Diagnostics and Radiopharmaceuticals, Faculty of Pharmacy, Medical University of Silesia, Sosnowiec, Poland.

Background: Talents are the basis of all development and comprehensive strength of a country. As college students who are about to contact the society, their mental health and social adaptability are the most critical factors for their entry into society. OBJECTIVE Therefore, this study explores the correlation of exercise style to mental health and social adaptability of college students.

Methods: Based on the sample processing of college students in 4 universities in a city, 1,000 students are selected for research on the correlation of exercise style to mental health and social adaptability. In addition, the impacts of college students participating in sports activities on their mental health and social adaptability are analyzed.

Results: When the duration of a single sports activity is 30-60 min, the mental health of students reaches optimal. On the contrary, when the duration of a single sports activity exceeds 90 min, the mental health of students is low. This indicates that the more students participate in sports activities, the stronger the social adaptability is. When the intensity of sports activities for students is moderate, the average scores of interpersonal communication ability of students are the highest. When the duration of a single sports activity is 60-90 min, the average scores of living adaptability of students are the highest. If students keep the appropriate duration and intensity of sports activities, their social adaptability will be improved notably.

Conclusion: The social adaptability of students is related to the number of times they participate in sports activities, the duration of a single sports activity, and the intensity of sports activities. However, while exercising, students should plan the duration of sports activities to avoid excessively prolonged exercise time. In addition, students should beware their exercise intensity of sports activities. The optimal choice of exercise intensity is moderate or slightly tired.
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http://dx.doi.org/10.3233/WOR-213497DOI Listing
June 2021

The relation between aortic arch branching types and the location of large vessel occlusion in cardioembolic stroke.

J Clin Neurosci 2021 Jul 12;89:171-176. Epub 2021 May 12.

Chongqing Key Laboratory of Cerebrovascular Disease Research, No. 439, Xuanhua Road, Yongchuan, Chongqing 402160, People's Republic of China; Department of Neurology, Yongchuan Hospital of Chongqing Medical University, No. 439, Xuanhua Road, Yongchuan, Chongqing 402160, People's Republic of China; Department of Internal Medicine, Changdu People's Hospital, Changdu 854000, People's Republic of China. Electronic address:

Cardiac embolism is the leading etiology of ischemic strokes. There are arguments about the left-right propensity of cardioembolic strokes.This study aimed to reveal the relationship between the different aortic arch types and the location of large vessel occlusion (LVO) in cardioembolic stroke.We retrospectively identified all patients with acute ischemic stroke admitted to our comprehensive stroke center who had medium- to high-risk cardioembolicsources according to the TOAST classification.Only those with LVO and available images of the aortic arch were included. Patients were classified into 3 groups according to the aortic arch types: Type I (n = 44), Type II (n = 105), Type III (n = 36).The thrombus was divided into large thrombus or small thrombus based on the location of LVO.Overall, left-sided strokes (50.8%) were almost equal to right-sided (49.2%). There was a growing tendency for the percentage of left-sided infarcts with advancement of the aortic arch types either in the total cases or in the atrial fibrillation cases, with no statistical difference between the 3 aortic arch types.In type III aortic arch, left-sided strokes (69.0%) were twice than right-sided (31%) in large thrombus (P < 0.05), while right-sided strokes (85.7%) were more common than left-sided (14.3%) in small thrombus (P < 0.05).Conversely, in type Ⅰ and II aortic arches, left-sided strokes were more common than right-sided in small thrombus, while right-sided strokes were more common than left-sided in large thrombus (P < 0.05). The left-right propensity of cardioembolic stroke is related to the proximity of clot lodging in different aortic arch types.
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http://dx.doi.org/10.1016/j.jocn.2021.05.013DOI Listing
July 2021

Mesoporous silica nanoparticles inflame tumors to overcome anti-PD-1 resistance through TLR4-NFκB axis.

J Immunother Cancer 2021 Jun;9(6)

State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Background: The clinical benefits of antiprogrammed cell death protein 1 (PD-1) therapy are compromised by resistance in immunologically cold tumors. Convergence of immunotherapy and bioengineering is potential to overcome the resistance. Mesoporous silica nanoparticles (MSNs) are considered the most promising inorganic biological nanomaterials for clinical transformation, however, the fundamental influence of MSNs on immunotherapy is unclear. In this study, we aimed to investigate the role of MSNs in tumor resensitization and explore the feasibility of MSNs combined with anti-PD-1 in cancer therapy.

Methods: Intrinsic and acquired resistant tumors, as well as spontaneous and secondary tumor recurrence models, were used to evaluate the influence of MSNs and the synergistical effect with anti-PD-1 therapy. The roles of CD8 cytotoxic T-lymphocytes (CTLs) and macrophages were assessed in Rag-1 mice, ovalbumin/OT-1 TCR transgenic T-cell system, and other blocking mice models. Mechanistic studies were processed by transcriptomics analysis and conducted in primary cells, in vitro coculture systems, and Toll-like receptor 4 (TLR4) knockout mice.

Results: Both granular and rod-shaped MSNs efficiently overcame tumor resistance with dependence on diameter and aspect ratio. Only once injection of MSNs in prior to anti-PD-1 markedly improved the treatment efficacy, protective immunity, and prognosis. MSNs per se boosted infiltration of CTLs as the early event (days 2-3); and synergistically with anti-PD-1 therapy, MSNs rapidly established a T cell-inflamed microenvironment with abundant high-activated (interferon-γ/tumor necrosis factor-α/Perforin/GranzymeB) and low-exhausted (PD-1/lymphocyte-activation gene 3 (LAG-3)/T-cell immunoglobulin and mucin-domain containing-3 (TIM-3)) CTLs. Chemokines Ccl5/Cxcl9/Cxcl10, which were produced predominantly by macrophages, promoted MSNs-induced CTLs infiltration. MSNs led to high Ccl5/Cxcl9/Cxcl10 production in vitro and in mice through regulating TLR4-NFκB axis. Blocking TLR4-NFκB axis in macrophages or CTLs infiltration abrogated MSNs-induced resensitization to anti-PD-1 therapy.

Conclusions: MSNs efficiently and rapidly inflame immunologically cold tumors and resensitize them to anti-PD-1 therapy through TLR4-NFκB-Ccl5/Cxcl9/Cxcl10 axis. MSNs-based theranostic agents can serve as sensitizers for patients with resistant tumors to improve immunotherapy.
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http://dx.doi.org/10.1136/jitc-2021-002508DOI Listing
June 2021

Prenatal exercise reprograms the development of hypertension progress and improves vascular health in SHR offspring.

Vascul Pharmacol 2021 Jun 9:106885. Epub 2021 Jun 9.

Department of Exercise Physiology, Beijing Sport University, Beijing 100084, China; Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University, Beijing, China. Electronic address:

Background: Upregulation of L-type voltage-gated Ca (Ca1.2) channel in the arterial myocytes is a hallmark feature of hypertension. However, whether maternal exercise during pregnancy has a sustained beneficial effect on the offspring of spontaneously hypertensive rats (SHRs) through epigenetic regulation of Ca1.2 channel is largely unknown.

Methods: Pregnant SHRs and Wistar-Kyoto rats were subjected to swimming and the vascular molecular and functional properties of male offspring were evaluated at embryonic (E) 20.5 day, 3 months (3 M), and 6 months (6 M).

Results: Exercise during pregnancy significantly decreased the resting blood pressure at 3 M but not 6 M in the offspring of SHR. Prenatal exercise significantly reduced the cardiovascular reactivity, the contribution of Ca1.2 channel to the vascular tone, and the whole-cell current density of Ca1.2 channel in both 3 M and 6 M offspring of SHR. Moreover, maternal exercise triggered hypermethylation of the promoter region of the Ca1.2 α1C gene (CACNA1C), with a concomitant decrease in its protein and mRNA expressions in SHR offspring at E20.5, 3 M, and 6 M. Tissue culture experiments further confirmed that 5-Aza-2'-deoxycytidine increased the structure and functional expression of Ca1.2 channel by inhibiting the DNA methylation of CACNA1C. However, the improvement of prenatal exercise on the blood pressure, function, and expression of Ca1.2 channel was attenuated in the offspring of SHRs at 6 M compared to the 3 M readout.

Conclusions: These data suggest that prenatal exercise improves the vascular function by the hypermethylation of CACNA1C in the arterial myocytes and delays the development of hypertension in the offspring of SHRs. However, these effects fade out with age.
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http://dx.doi.org/10.1016/j.vph.2021.106885DOI Listing
June 2021

Set2-mediated H3K36 methylation states redundantly repress the production of antisense transcripts: role in transcription regulation.

FEBS Open Bio 2021 Jun 11. Epub 2021 Jun 11.

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, RNA Institute, Wuhan University, Wuhan, 430072, China.

Methyltransferase Set2-mediated methylation of histone H3 lysine 36 (H3K36), which involves the addition of up to three methyl groups at this site, has been demonstrated to function in many chromatin-coupled events. The methylation of H3K36 is known to recruit different chromatin effector proteins, affecting transcription, mRNA splicing and DNA repair. In this study, we engineered two yeast set2 mutants that lack H3K36 mono/dimethylation (H3K36me1/2) and trimethylation (H3K36me3), respectively, and characterized their roles in the production of antisense transcripts under nutrient-rich conditions. Using our new bioinformatics identification pipeline analysis, we are able to identify a larger number of antisense transcripts in set2∆ cells than has been published previously. We further show that H3K36me1/2 or H3K36me3 redundantly repressed the production of antisense transcripts. Moreover, gene ontology (GO) analysis implies that H3K36me3-mediated antisense transcription might play a role in DNA replication and DNA damage repair, which is independent of regulation of the corresponding sense gene expression. Overall, our results validate a coregulatory mechanism of different H3K36 methylation states, particularly in the repression of antisense transcription.
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http://dx.doi.org/10.1002/2211-5463.13226DOI Listing
June 2021

Single-cell RNA sequencing of human femoral head .

Aging (Albany NY) 2021 Jun 10;13(11):15595-15619. Epub 2021 Jun 10.

Center for System Biology, Data Sciences, and Reproductive Health, School of Basic Medical Science, Central South University, Yuelu, Changsha 410013, China.

The homeostasis of bone metabolism depends on the coupling and precise regulation of various types of cells in bone tissue. However, the communication and interaction between bone tissue cells at the single-cell level remains poorly understood. Thus, we performed single-cell RNA sequencing (scRNA-seq) on the primary human femoral head tissue cells (FHTCs). Nine cell types were identified in 26,574 primary human FHTCs, including granulocytes, T cells, monocytes, B cells, red blood cells, osteoblastic lineage cells, endothelial cells, endothelial progenitor cells (EPCs) and plasmacytoid dendritic cells. We identified () and () as novel bone metabolism-related genes. Additionally, we found that several subtypes of monocytes, T cells and B cells were related to bone metabolism. Cell-cell communication analysis showed that collagen, chemokine, transforming growth factor and their ligands have significant roles in the crosstalks between FHTCs. In particular, EPCs communicated with osteoblastic lineage cells closely via the "COL2A1-ITGB1" interaction pair. Collectively, this study provided an initial characterization of the cellular composition of the human FHTCs and the complex crosstalks between them at the single-cell level. It is a unique starting resource for in-depth insights into bone metabolism.
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http://dx.doi.org/10.18632/aging.203124DOI Listing
June 2021

Anatomical Variations of the Vertebral Artery: Analysis by Three-Dimensional Computed Tomography Angiography in Chinese Population.

Orthop Surg 2021 Jun 8. Epub 2021 Jun 8.

Department of Orthopedics, General Hospital of Northern Theater Command, Shenyang, China.

Objective: To analyze the anatomical variations of V3 and V2 segments of the vertebral artery in the Chinese population.

Methods: The current retrospective study was an observational, anatomical, radiological research. Between 1 January 2018 to 31 December 2019, the data of 589 continuous head-and-neck three-dimensional computed tomography angiography were observed and analyzed using the open-source software of Horos. There were 415 males and 174 females with an average age of 44.63 ± 2.5(18-74) years. The variations of the V3 segment were defined as persistent first intersegmental artery (PFIA) and paracondylar processes (PP). The variations of V2 segments were unusual vertebral artery entrance transverse foreman (UE-V2S) and midline migration (MM). The incidences of all variations were summarized and the gender, side characters were compared.

Results: Among the patients, 4.34% (18/415) males and 4.60% (8/174) females were with the variation of PFIA. Meanwhile, 12.29% (51/415) males and 10.92% (19/174) females were with the variation of PP. Then 18.80% (78/415) males and 16.67% (29/174) females were with UE-V2S. All the variations above were not different in genders. As for the variation of MM, 3.86% (16/415) males and 8.62% (15/174) females were identified, and the difference of genders was significant for this type of variation (P < 0.05). The differences between each side were also observed and analyzed for all variations and no differences were found.

Conclusion: There are several variations of V3 and V2 segments of the vertebral artery are associated with the cervical surgeries, most were without differences in genders and sides, except the variation of MM.
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http://dx.doi.org/10.1111/os.13047DOI Listing
June 2021

Virtual screening on the web for drug repurposing: a primer.

J Biol Methods 2021 26;8(2 COVID 19 Spec Iss):e148. Epub 2021 May 26.

Department of Applied Biology and Chemical Technology and State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hunghom, Hong Kong, China.

We describe a procedure of performing (virtual) screening using a web-based service, the MTiOpenScreen, which is freely accessible to non-commercial users. We shall use the SARS-CoV-2 main protease as an example. Starting from a structure downloaded from the Protein Data Bank, we discuss how to prepare the coordinates file, taking into account the known biochemical background information of the target protein. The reader will find that this preparation step takes up most of the effort before the target is ready for screening. The steps for uploading the target structure and defining the search volume by critical residues, and the main parameters to use, are outlined. When this protocol is followed, the user will expect to obtain a ranked list of small approved drug compounds docked into the target structure. The results can be readily examined graphically on the web site or downloaded for studying in a local molecular graphics program such as PyMOL.
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http://dx.doi.org/10.14440/jbm.2021.351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175336PMC
May 2021

Prevalence of elder abuse and victim-related risk factors during the COVID-19 pandemic in China.

BMC Public Health 2021 06 8;21(1):1096. Epub 2021 Jun 8.

Law School, Hunan University, Changsha, Hunan Province, P.R. China.

Background: With the accelerated aging of the Chinese population, elder abuse has become a serious social problem. As COVID-19 has had a very large impact on economic development and lifestyle in China, it has also affected elder abuse. The purpose of this study is to estimate the prevalence of elder abuse in China during the COVID-19 pandemic, and to identify changes in risk factors for elder abuse in the context of COVID-19.

Methods: We designed a cross-sectional study. In Hunan Province, a face-to-face questionnaire survey was conducted among elderly people over 65 years of age. To ensure the consistency of the measurement standards, we used the elder abuse questionnaire from the "Third Survey on Chinese Women's Social Status." According to related research, we selected 10 victim-related risk factors as independent variables. A logistic regression model was established to analyze the relationship between the independent variables and the four kinds of abuse.

Results: We collected 10,362 samples from Hunan Province. During the COVID-19 pandemic, the prevalence of financial abuse and neglect was significantly higher than that in 2010. Income had a significant impact on the four types of abuse. The lower the income was, the greater the risk of abuse. Moreover, factors such as an older age, being a woman, a lower cognitive ability, and not having a cohabiting spouse increased the possibility of abuse. The greater the number of children was, the greater the risks of physical abuse, financial abuse, and elder neglect. Seniors with higher education levels, those who frequently participated in social activities, and those with religious beliefs were less likely to suffer abuse.

Conclusions: During the COVID-19 epidemic, the prevalence of elder abuse in China has increased, which may be related to economic instability and social distancing measures. Increasing the income of the elderly and giving them more social support are important measures to reduce the prevalence of elder abuse.
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http://dx.doi.org/10.1186/s12889-021-11175-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185499PMC
June 2021