Publications by authors named "Young-Hyo Chang"

70 Publications

sp. nov., isolated from the cecum of a mini-pig.

Int J Syst Evol Microbiol 2019 Jun 16;71(3). Epub 2021 Mar 16.

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

A rod-shaped, facultative anaerobic, Gram-stain-positive bacteria, isolated from the cecum of a mini-pig, was designated as strain YH-lac23. Analysis of 16S rRNA gene sequences revealed that the strain was closely related to JCM 33273 (97.9 %), KCTC 21027 (96.2 %) and KCTC 21010 (95.7 %). Analysis of housekeeping gene sequences ( and ) revealed that the strain formed a sub-cluster with . The average nucleotide identity value for YH-lac23 and its most closely related strain () is 80.7 %. The main fatty acids are Cω9 and C. The cell wall contains the peptidoglycan of -diaminopimelic acid. The G+C content of the genomic DNA is 59.8 mol%. In view of the chemotaxonomic, phenotypic and phylogenetic properties, YH-lac23 (=KCTC 25006=JCM 33998) represents a novel taxon. The name sp. nov. is proposed.
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http://dx.doi.org/10.1099/ijsem.0.004752DOI Listing
June 2019

Description of p. nov., a new bacterium isolated from soil.

Int J Syst Evol Microbiol 2019 Jun 17;71(3). Epub 2021 Feb 17.

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Two strains isolated from soil samples were designated as YH-JAE5 and YH-JAE2. The isolates were facultative anaerobic, Gram-stain-variable, motile, rod-shaped bacteria. Phylogenetic analysis indicated that the isolates belonged to the genus , but the 16S rRNA gene sequence similarities were <98 % when compared with other species within the genus. Analysis of gene revealed the isolates formed a sub-cluster with . The only menaquinone identified was MK-7. The two isolates contained -diaminopimelic acid within their cell wall peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, aminophospholipids, and lipids. The major fatty acids were C anteiso and C iso. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between isolate YH-JAE5 and the most closely related reference strain ( KCTC 3758) were 81.7, 84.8 and 23.4 %, respectively. The G+C content of the genomic DNA was 47.4 mol%. Thus, the polyphasic data revealed that YH-JAE2 (=KCTC 43239=JCM 34435) and YH-JAE5 (=KCTC 43059=JCM 33533) represent a new species. The name sp. nov. is proposed.
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http://dx.doi.org/10.1099/ijsem.0.004707DOI Listing
June 2019

sp. nov., isolated from the large intestine of a mini-pig.

Int J Syst Evol Microbiol 2019 Jun 8;71(3). Epub 2021 Feb 8.

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

An obligately anaerobic, Gram-stain-negative, spore-forming, short rod-shaped bacterium, designated strain YH- T4B42, was isolated from the large intestine of a mini-pig. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus and is most closely related to KCTC 5424, KCTC 15329 and KCTC 1871, with 95.5, 92.4 and 83.0 % sequence similarity, respectively. The average nucleotide identity values for strain YH-T4B42 and the closest related strains were lower than 72 %. The G+C content of the isolate was 55.8 mol%. The cell-wall peptidoglycan was A1γ type and contained -diaminopimelic acid. The predominant fatty acids were C, C 9, C and C. The major end products of glucose fermentation were lactate, formate and acetate, with a minor amount of butyrate. Based on its phenotypic, phylogenetic and chemotaxonomic properties, a novel species, sp. nov., is proposed for strain YH-T4B42 (=KCTC 25105=NBRC 114767).
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http://dx.doi.org/10.1099/ijsem.0.004694DOI Listing
June 2019

sp. nov., isolated from the small intestine of a mini pig.

Int J Syst Evol Microbiol 2020 Dec;70(12):6476-6481

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

A Gram-stain-positive, facultative anaerobic, rod-shaped bacteria isolated from the small intestine of a mini pig was designated as strain YH-lac9. 16S rRNA gene sequence analysis revealed that the strain belongs to the genus and is closely related to JCM 17472, JCM 15042 and JCM 13927, with 97.6, 96.2 and 95.7 % sequence similarity, respectively. Analysis of housekeeping gene sequences ( and ) revealed that the strain formed a sub-cluster with , supporting the results of 16S rRNA gene sequences analysis. The average nucleotide identity value for YH-lac9 and the most closely related strain is 74.1 %. The main fatty acids are Cω9, summed feature 7, C and summed feature 8. The G+C content of the genomic DNA is 37.8 mol%. In view of its chemotaxonomic, phenotypic and phylogenetic properties, YH-lac9 (=KCTC 25005=JCM 33997) represents a novel taxon. The name sp. nov. is proposed.
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http://dx.doi.org/10.1099/ijsem.0.004560DOI Listing
December 2020

Correction to: Genome-Based Reclassification of Fusobacterium nucleatum Subspecies at the Species Level.

Curr Microbiol 2020 Nov;77(11):3807

ABS Research Support Center, KRIBB, Daejeon, Republic of Korea.

The original version of this article contained errors in the description of novel species. These errors are corrected with this corrigendum.
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http://dx.doi.org/10.1007/s00284-020-02133-wDOI Listing
November 2020

Paenibacillus oralis sp. nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion.

Curr Microbiol 2020 Mar 12;77(3):509-515. Epub 2019 Dec 12.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20, was isolated from the human subgingival dental plaque of a gingivitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus. BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593 (97.7% similarity) and Paenibacillus macerans ATCC 8244 (97.6% similarity). Draft genome of strain ChDC PVNT-B20 was composed of 8,112,407 bp. The DNA G+C content of the strain was 51.3 mol%. Average nucleotide identity values between strain ChDC PVNT-B20 and P. faecis DSM 23593 or P. macerans ATCC 8244 were 75.71% and 91.5%, respectively. Genome-to-genome distance values between strain ChDC PVNT-B20 and P. faecis DSM 23593 or P. macerans ATCC 8244 were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively. Major cellular fatty acids of strain ChDC PVNT-B20 were anteiso-C (43.4%), C (16.6%), iso-C (14.4%), and anteiso-C (12.4%). The sole respiratory quinone of the strain was menaqinone-7. Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL). Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3). Based on these results, strain ChDC PVNT-B20 is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp. nov. is proposed. Type strain is ChDC PVNT-B20 (= KCOM 3021 = JCM 33462).
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http://dx.doi.org/10.1007/s00284-019-01843-0DOI Listing
March 2020

gen. nov., sp. nov., a member of the family isolated from pig faeces.

Int J Syst Evol Microbiol 2020 Feb;70(2):732-737

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.

An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20, was isolated from pig faeces. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family , and is most closely related to KCTC 15517 (93.5 % sequence similarity), followed by KCTC 5815 (92.2 %), KCTC 15521 (90.2 %) and KCTC 5969 (89.6 %). Average nucleotide identity values between YH-panp20 and its closest relatives were lower than 71 %. The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained -diaminopimelic acid. The predominant fatty acids were C 9, C DMA and C. The major end-products of glucose fermentation were lactate, acetate and formate. Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, gen. nov., sp. nov., is proposed for isolate YH-panp20 (=KCTC 15747=JCM 32769).
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http://dx.doi.org/10.1099/ijsem.0.003803DOI Listing
February 2020

Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.

Curr Microbiol 2019 Dec 30;76(12):1531-1536. Epub 2019 Sep 30.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high similarity with Streptococcus rubneri DSM 26920 (98.6%), Streptococcus parasanguinis ATCC 15912 (98.5%), and Streptococcus australis CCUG 45919 (98.3%). The genome of strain JS71 was 2,009,592 bp in length. The DNA G+C content of the strain was 42.1 mol%. Average nucleotide identity values between strain JS71 and S. rubneri DSM 26920, S. parasanguinis ATCC 15912, and S. australis CCUG 45919 were 88.9%, 80.8%, and 92.4%, respectively. Genome-to-genome distance values between strain JS71S. rubneri DSM 26920, S. parasanguinis ATCC 15912, and S. australis CCUG 45919 were 36.5% (34-39%), 26.3% (23.9-28.7%), and 48.0% (45.4-50.6%), respectively. The major fatty acids of the strain were C (39.7%), C/C (15.5%), and C (10.4%). Based on these results, strain JS71 (= KCOM 2890 = JCM 33454) should be a novel species of the genus Streptococcus, for which the name Streptococcus koreensis sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-019-01778-6DOI Listing
December 2019

Lautropia dentalis sp. nov., Isolated from Human Dental Plaque of a Gingivitis Lesion.

Curr Microbiol 2019 Nov 24;76(11):1369-1373. Epub 2019 Aug 24.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 was isolated from human subgingival dental plaque of a gingivitis lesion. The phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) sequence showed that the strain belonged to the genus Lautropia. 16S rDNA of strain ChDC F240 had the highest similarity to that of Lautropia mirabilis ATCC 51599 (98.8%). Major cellular fatty acids of strain ChDC F240 were C (43.9%) and C/C (38.1%). Draft genome of the strain was 3,834,139 bp in length and the G+C content was 65.0 mol%. Average nucleotide identity and genome-to-genome distance values between strain ChDC F240 and L. mirabilis ATCC 51599 were 81.99% and 28.50% (26.1-30.9%), respectively. These results reveal that strain ChDC F240 is a novel species within the genus Lautropia, for which the name Lautropia dentalis sp. nov. is proposed; type strain is ChDC F240 (= KCOM 2505 = JCM 33297).
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http://dx.doi.org/10.1007/s00284-019-01761-1DOI Listing
November 2019

Lachnoanaerobaculum gingivalis sp. nov., Isolated from Human Subgingival Dental Plaque of a Gingivitis Lesion.

Curr Microbiol 2019 Oct 26;76(10):1147-1151. Epub 2019 Jul 26.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel Gram-stain-positive, obligately anaerobic, spore-forming rod, designated strain ChDC B114, was isolated from a human dental plaque of a gingivitis lesion. The strain was characterized by polyphasic taxonomic analysis to identify it at the species level. The 16S ribosomal RNA gene (16S rDNA) sequence analysis revealed that the strain belongs to the genus Lachnoanaerobaculum. The percent similarity of the 16S rDNA of the strain was closest to the homologous gene sequence of Lachnoanaerobaculum orale N1 (98.5%) and Lachnoanaerobaculum saburreum CCUG 28089 (97.6%). The major fatty acids of strain ChDC B114 were C (30.7%), C (17.7%), iso-C (14.9%), and C 2OH (12.0%). The draft genome of strain ChDC B114 was 3,097,953 bp in length. The G+C content of the strain was 35.9 mol %. Average nucleotide identity values between strain ChDC B114 and L. orale N1 and L. saburreum CCUG 28089 were 83.2% and 82.0%, respectively. Genome-to-genome distance values between strain ChDC B114 and L. orale N1 and L. saburreum CCUG 28089 were 26.8% (24.5-29.3%) and 26.30% (24.0-28.8%), respectively. Based on these results, strain ChDC B114 (= KCOM 2030 = JCM 33452) should be classified as a novel species of genus Lachnoanaerobaculum, for which the name Lachnoanaerobaculum gingivalis sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-019-01747-zDOI Listing
October 2019

Streptococcus chosunense sp. nov., Isolated from Human Postoperative Maxillary Cyst.

Curr Microbiol 2019 Oct 22;76(10):1193-1198. Epub 2019 Jul 22.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel facultative anaerobic, non-spore forming, non-motile, and Gram-stain-positive coccus, designated strain ChDC B353, was isolated from human postoperative maxillary cyst. The 16S ribosomal RNA gene (16S rDNA) sequence of the strain was most closely related to those of Streptococcus pseudopneumoniae ATCC BAA-960 (99.4%), Streptococcus mitis NCTC 12261 (99.3%), and Streptococcus pneumoniae NCTC 7465 (99.2%). The major fatty acids of the strain were C (43.2%) and C/C (20.2%). The genome of strain ChDC B353 was composed of 1,902,053 bps. The DNA G+C content of the strain was 40.2 mol%. Average nucleotide identity (ANI) values between strain ChDC B353 and S. pseudopneumoniae ATCC BAA-960, S. mitis NCTC 12261, and S. pneumoniae NCTC 7465 were 91.9%, 93.5%, and 91.3%, respectively. Genome-to-genome distance (GGD) values between strain ChDC B353 and S. pseudopneumoniae ATCC BAA-960, S. mitis NCTC 12261, or S. pneumoniae NCTC 7465 were 46.6% (44.0-49.2%), 53.2% (50.5-55.9%), and 46.0% (43.5-48.7%), respectively. The threshold values of ANI and GGD for species discrimination are 95-96% and 70%, respectively. These results reveal that strain ChDC B353 (= KCOM 1699 = JCM 33453) is a novel species belonging to genus Streptococcus, for which a name of Streptococcus chosunense sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-019-01746-0DOI Listing
October 2019

Prevotella koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.

Curr Microbiol 2019 Sep 18;76(9):1055-1060. Epub 2019 Jun 18.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel Gram-negative, obligately anaerobic, non-motile, non-spore forming, and rod-shaped bacterium, designated strain JS262, was isolated from human subgingival plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. Comparison of 16S ribosomal RNA gene (16S rDNA) sequence revealed that the strain belonged to the genus Prevotella. The percent similarity of 16S rDNA of strain JS262 was closest to those of Prevotella buccae ATCC 33574 (89.1%) and Prevotella shahii JCM 12083 (88.9%). The major fatty acids of strain JS262 were C (29.2%), iso-C (19.2%), and anteiso-C (16.9%). Complete genome of strain JS262 was 2,691,540 bp in length and the G+C content was 43.9 mol%. Average nucleotide identity and genome-to-genome distance values between strain JS262 and P. buccae ATCC 33574 or P. loescheii DSM 19665 were > 70.4% and > 30.1%, respectively. On the basis of these data, a novel Prevotella species is proposed: Prevotella koreensis sp. nov. The type strain of P. koreensis is JS262 (= KCOM 3155 = JCM 33298).
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http://dx.doi.org/10.1007/s00284-019-01720-wDOI Listing
September 2019

Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.

Curr Microbiol 2019 Jul 3;76(7):835-841. Epub 2019 May 3.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel facultative anaerobic and Gram-stain-positive coccus, designated strain ChDC F135, was isolated from human subgingival dental plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene (16S rDNA) sequence of strain ChDC F135 was closest to that of Streptococcus sinensis HKU4 (98.2%), followed by Streptococcus intermedia SK54 (97.0%), Streptococcus constellatus NCTC11325 (96.0%), and Streptococcus anginosus NCTC 10713 (95.7%). In contrast, phylogenetic analysis based on the superoxide dismutase gene (sodA) and the RNA polymerase beta-subunit gene (rpoB) showed that the nucleotide sequence similarities of strain ChDC F135 were highly similar to the corresponding genes of S. anginosus NCTC 10713 (99.2% and 97.6%, respectively), S. constellatus NCTC11325 (87.8% and 91.4%, respectively), and S. intermedia SK54 (85.8% and 91.2%, respectively) rather than those of S. sinensis HKU4 (80.5% and 82.6%). The complete genome of strain ChDC F135 consisted of 1,901,251 bp and the G+C content was 38.9 mol %. Average nucleotide identity value between strain ChDC F135 and S. sinensis HKU4 or S. anginosus NCTC 10713 were 75.7% and 95.6%, respectively. The C composition of the cellular fatty acids of strain ChDC F135 (32.8%) was different from that of S. intermedia (6-8%), S. constellatus (6-13%), and S. anginosus (13-20%). Based on the results of phylogenetic and phenotypic analysis, strain ChDC F135 (= KCOM 2412 = JCM 33300) was classified as a type strain of a novel species of the genus Streptococcus, for which we proposed the name Streptococcus periodonticum sp. nov.
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http://dx.doi.org/10.1007/s00284-019-01695-8DOI Listing
July 2019

Streptococcus gwangjuense sp. nov., Isolated from Human Pericoronitis.

Curr Microbiol 2019 Jul 26;76(7):799-803. Epub 2019 Apr 26.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel facultative anaerobic, Gram-stain-negative coccus, designated strain ChDC B345, was isolated from human pericoronitis lesion and was characterized by polyphasic taxonomic analysis. The 16S ribosomal RNA gene (16S rDNA) sequence revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence of strain ChDC B345 was most closely related to those of  Streptococcus mitis NCTC 12261 (99.5%) and Streptococcus pseudopneumoniae ATCC BAA-960 (99.5%). Complete genome of strain ChDC B345 was 1,972,471 bp in length and the G + C content was 40.2 mol%. Average nucleotide identity values between strain ChDC B345 and S. pseudopneumoniae ATCC BAA-960 or S. mitis NCTC 12261 were 92.17% and 93.63%, respectively. Genome-to-genome distance values between strain ChDC B345 and S. pseudopneumoniae ATCC BAA-960 or S. mitis NCTC 12261 were 47.8% (45.2-50.4%) and 53.0% (51.0-56.4%), respectively. Based on these results, strain ChDC B345 (= KCOM 1679= JCM 33299) should be classified as a novel species of genus Streptococcus, for which we propose the name Streptococcus gwangjuense sp. nov.
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http://dx.doi.org/10.1007/s00284-019-01687-8DOI Listing
July 2019

Fusobacterium pseudoperiodonticum sp. nov., Isolated from the Human Oral Cavity.

Curr Microbiol 2019 Jun 1;76(6):659-665. Epub 2019 Apr 1.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

In the present study, three strains (ChDC F213, ChDC F251, and ChDC F267) were classified as novel species of genus Fusobacterium based on average nucleotide identity (ANI) and genome-to-genome distance (GGD) analysis and chemotaxonomic characterization. 16S rDNA sequences of strains ChDC F213, ChDC F251, and ChDC F267 were highly similar to that of F. periodonticum ATCC 33693 (99.6, 99.4, and 99.4%, respectively). ANI and GGD values of the three isolates with F. periodonticum ATCC 33693 ranged from 92.5 to 92.6% and 47.7 to 48.2%, respectively. Considering that threshold of ANI and GGD values for bacterial species discrimination are 95-96% and 70%, respectively, these results indicate that the three isolates represent a novel Fusobacterium species. DNA G + C contents of the three isolates were 28.0 mol% each. Cellular fatty acid analysis of these strains revealed that C, C, and C ω6c/C ω7c were major fatty acids. Therefore, these three strains are novel species belonging to genus Fusobacterium. Strain ChDC F213 (= KCOM 1259 = KCTC 5677 = JCM 33009) is the type strain of a novel species of genus Fusobacterium, for which a name of Fusobacterium pseudoperiodonticum sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-019-01675-yDOI Listing
June 2019

Blautia argi sp. nov., a new anaerobic bacterium isolated from dog faeces.

Int J Syst Evol Microbiol 2019 Jan 8;69(1):33-38. Epub 2018 Nov 8.

1​ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.

Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15 and YH1_16, were isolated from faecal samples obtained from a mature dog. Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951, Blautia stercoris KCTC 5981, Blautia producta producta KCTC 3695 and B. coccoides DSM 15327, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively. The two isolates contained m-diaminopimelic acid within their peptidoglycans. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %). The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%. Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp. nov. The type strain is N6H1-15 (=KCTC 15426=JCM 31394).
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http://dx.doi.org/10.1099/ijsem.0.002981DOI Listing
January 2019

Clostridium composti sp. nov., a new anaerobic bacteria isolated from compost.

Int J Syst Evol Microbiol 2018 Dec 25;68(12):3869-3873. Epub 2018 Oct 25.

1​ABS Research Support Center/Korean Collection for Type Cultures, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.

Strain YH-tan31 is a Gram-stain-positive, obligately anaerobic, spore-forming and mesophilic bacterium. The cells are rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium oryzae DSM 28571 (97 % sequence identity), followed by Clostridium cellulovorans DSM 3052 (92.4 %) and Clostridium butyricum KCTC 5387 (92.1 %). The G+C content of the isolate was 30.9 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids were C14 : 0, C16 : 0, C16 : 0 DMA,and C19 : 0cis 11,12 DMA. The major end products of glucose fermentation were butyrate, acetate and lactate. This isolate represents a new species within the genus Clostridium, for which we propose the name Clostridiumcomposti sp. nov. (type strain, YH-tan31; =KCTC 15630=JCM 32793).
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http://dx.doi.org/10.1099/ijsem.0.003074DOI Listing
December 2018

Capnocytophaga endodontalis sp. nov., Isolated from a Human Refractory Periapical Abscess.

Curr Microbiol 2018 Apr 27;75(4):420-425. Epub 2017 Nov 27.

Korean Collection for Oral Microbology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel Gram-negative, capnophilic, fusiform bacterium, designated strain ChDC OS43, was isolated from a human refractory periapical abscess in the left mandibular second molar and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene sequence revealed that the strain belongs to the genus Capnocytophaga, as it showed sequence similarities to Capnocytophaga ochracea ATCC 27872 (96.30%) and C. sputigena ATCC 33612 (96.16%). The prevalent fatty acids of strain ChDC OS43 were isoC (57.54%), C (5.93%), C 3OH (5.72%), and C cis 9 (4.41%). The complete genome of strain ChDC OS43 was 3,412,686 bp, and the G+C content was 38.2 mol%. The average nucleotide identity (ANI) value between strain ChDC OS43 and C. ochracea ATCC 27872 or C. sputigena ATCC 33612 was >92.01%. The genome-to-genome distance (GGD) value between strain ChDC OS43 and C. ochracea ATCC 27872 or C. sputigena ATCC 33612 was 32.0 and 45.7%, respectively. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC OS43 (= KCOM 1579 = KCTC 5562 = KCCM 42841 = JCM 32133) should be classified as the type strain of a novel species of genus Capnocytophaga, for which the name Capnocytophaga endodontalis sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-017-1397-5DOI Listing
April 2018

Bacteroides koreensis sp. nov. and Bacteroides kribbi sp. nov., two new members of the genus Bacteroides.

Int J Syst Evol Microbiol 2017 Nov 25;67(11):4352-4357. Epub 2017 Sep 25.

ABS Research Support Center/Korean Collection for Type Cultures, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.

Three bacterial isolates from human faeces, YS-aM39, R2F3-3-3 and R2F3-5-1, were characterized as Gram-negative, strictly anaerobic, non-spore-forming, non-motile, and rod-shaped. Isolate YS-aM39 formed a distinct line of descent, showing greatest 16S rRNA gene sequence relatedness with R2F3-3-3 (97.5 %), R2F3-5-1 (97.5 %), Bacteroides ovatus (98.8 %) and Bacteroides xylanisolvens (97.2 %). Isolates R2F3-3-3 and R2F3-5-1 also formed a distinct line of descent, sharing greatest 16S rRNA gene sequence relatedness with B. ovatus (98.2 %) and B. xylanisolvens (97.2 %). The DNA G+C content of YS-aM39 was 44.8 mol%, that of R2F3-3-3 was 42.4 mol% and that of R2F3-5-1 was 42.6 mol%. The respiratory quinone of all three isolates was menaquinone MK-10. Polar lipid analysis identified phosphatidylethanolamine as the major lipid. The predominant fatty acids in all three isolates were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 3-OH and iso-C17 : 0 3-OH. The major end products of glucose fermentation were acetic acid, lactic acid and formic acid. DNA-DNA hybridization data indicated that two isolates, YS-aM39 and R2F3-3-3, represent a species distinct from B. ovatus and B. xylanisolvens. Finally, in this study, the two isolates represented two new species in the genus Bacteroides, for which we propose the names Bacteroides koreensis sp. nov. (type strain, YS-aM39=KCTC 15520=JCM 31393) and Bacteroides kribbi sp. nov. (type strain, R2F3-3-3=KCTC 15460=JCM 31391).
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http://dx.doi.org/10.1099/ijsem.0.002226DOI Listing
November 2017

Description of Absiella argi gen. nov., sp. nov., and transfer of Eubacterium dolichum and Eubacterium tortuosum to the genus Absiella as Absiella dolichum comb. nov. and Absiella tortuosum comb. nov.

Anaerobe 2017 Dec 25;48:70-75. Epub 2017 Jul 25.

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. Electronic address:

Gram-positive, straight or slightly curved rod-shaped bacteria, designated as strains N6H1-5 and N6H1-3, were isolated from fecal samples of old dog. The analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Clostridium cluster XVI and were closely related to Eubacterium dolichum KCTC 5832, Eubacterium tortuosum DSM 3987, Clostridium innocuum KCTC 5183, Allobaculum stecoricanis DSM 13633, Eubacterium limosum KCTC 3266, and Clostridium butyricum KCTC 1871, with 94.0%, 93.8%, 92.0%, 84.9%, 80.7%, and 80.0% sequence similarity, respectively. Chemotaxonomic data supported placement of the strains N6H1-5 and N6H1-3 in the new taxon. The strains contained m-diaminopimelic acid cell wall peptidoglycan; the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and glycolipids (GL); and the major fatty acids were Ccis 9 (30.7%) and C (17.1%). The predominant metabolic end product was lactic acid. The G + C content was 35.8 mol%. The most closely related species, E. dolichum and E. tortuosum, were also assigned to the new taxon, based on the phylogenetic analysis and phenotypic data. Thus, the type strain N6H1-5 (=KCTC 15422 = JCM 30884) represents a novel genus and species, for which the name Absiella argi gen. nov., sp. nov is proposed. It is also proposed that E. dolichum KCTC 5832 and E. tortuosum DSM 3987 be transferred to this new genus, and named Absiella dolichum comb. nov. and Absiella tortuosum comb. nov., respectively.
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http://dx.doi.org/10.1016/j.anaerobe.2017.07.006DOI Listing
December 2017

Genome-Based Reclassification of Fusobacterium nucleatum Subspecies at the Species Level.

Curr Microbiol 2017 Oct 7;74(10):1137-1147. Epub 2017 Jul 7.

ABS Research Support Center, KRIBB, Daejeon, Republic of Korea.

Fusobacterium nucleatum is classified as four subspecies, subsp. nucleatum, polymorphum, vincentii, and animalis, based on DNA-DNA hybridization (DDH) patterns, phenotypic characteristics, and/or multilocus sequence analysis (MLSA). The gold standards for classification of bacterial species are DDH and 16S ribosomal RNA gene (16S rDNA) sequence homology. The thresholds of DDH and 16S rDNA similarity for delineation of bacterial species have been suggested to be >70 and 98.65%, respectively. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) analysis based on genome sequences were recently introduced as a replacement for DDH to delineate bacterial species with ANI (95-96%) and GGD (70%) threshold values. In a previous study, F. hwasookii was classified as a new species based on MLSA and DDH results. 16S rDNA similarity between F. hwasookii type strain and F. nucleatum subspecies type strains was higher than that between F. nucleatum subspecies type strains. Therefore, it is possible that the four F. nucleatum subspecies can be classified as Fusobacterium species. In this study, we performed ANI and GGD analyses using the genome sequences of 36 F. nucleatum, five F. hwasookii, and one Fusobacterium periodonticum strain to determine whether the four F. nucleatum subspecies could be classified as species using OrthoANI and ANI web-based softwares provided by ChunLab and Kostas lab, respectively, and GGD calculator offered by German Collection of Microorganisms and Cell Cultures. ANI values calculated from OrthoANI and ANI calculators between the type strains of F. nucleatum subspecies ranged from 89.80 to 92.97 and from 90.40 to 91.90%, respectively. GGD values between the type strains of F. nucleatum subspecies ranged from 42.3 to 46.0%. ANI and GGD values among strains belonging to the same F. nucleatum subspecies, subsp. nucleatum, subsp. polymorphum, subsp. vincentii, and subsp. animalis were >96 and >68.2%, respectively. These results strongly suggest that F. nucleatum subsp. nucleatum, subsp. polymorphum, subsp. vincentii, and subsp. animalis should be classified as F. nucleatum, F. polymorphum, F. vincentii, and F. animalis, respectively.
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http://dx.doi.org/10.1007/s00284-017-1296-9DOI Listing
October 2017

Vibrio injenensis sp. nov., isolated from human clinical specimens.

Antonie Van Leeuwenhoek 2017 Jan 23;110(1):145-152. Epub 2016 Dec 23.

Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.

Vibrio species are well known as motile, mostly oxidase-positive, facultative anaerobic Gram-negative bacteria. They are abundant in aquatic environments and are a common cause of human infections including diarrhea, soft tissue diseases, and bacteremia. Here, two Gram-negative bacteria, designated M12-1144 and M12-1181, were isolated from human clinical specimens and identified using a polyphasic taxonomic approach. Phylogenetic study based on 16S rRNA gene sequence analysis revealed that the isolates belong to the genus Vibrio, and are closely related to Vibrio metschnikovii KCTC 32284 (98.3%) and Vibrio cincinnatiensis KCTC 2733 (97.8%). The major fatty acids were summed feature 3 (C ω7c/C ω6c, 38.0%), C (23.0%), and summed feature 8 (C ω7c or C ω6c, 19.3%) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The G + C content of the genomic DNA was determined to be 44.1 mol%. DNA-DNA relatedness between the two newly isolated strains and V. metschnikovii KCTC 32284 and V. cincinnatiensis KCTC 2733 was between 42.6 to 47.5%. The similarities of genome-to-genome distance between M12-1144 and related species ranged from 18.4-54.8%. Based on these results, a new species of the genus Vibrio, Vibrio injenensis is proposed. The type strain is M12-1144 (=KCTC 32233 =JCM 30011).
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http://dx.doi.org/10.1007/s10482-016-0810-6DOI Listing
January 2017

Development and Evaluation of Species-Specific PCR for Detection of Nine Acinetobacter Species.

Ann Clin Lab Sci 2016 May;46(3):270-8

Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.

Molecular methods have the potential to improve the speed and accuracy of Acinetobacter species identification in clinical settings. The goal of this study is to develop species-specific PCR assays based on differences in the RNA polymerase beta-subunit gene (rpoB) to detect nine commonly isolated Acinetobacter species including Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter pittii, Acinetobacter nosocomialis, Acinetobacter lwoffii, Acinetobacter ursingii, Acinetobacter bereziniae, Acinetobacter haemolyticus, and Acinetobacter schindleri. The sensitivity and specificity of these nine assays were measured using genomic DNA templates from 55 reference strains and from 474 Acinetobacter clinical isolates. The sensitivity of A. baumannii-specific PCR assay was 98.9%, and the sensitivity of species-specific PCR assays for all other species was 100%. The specificities of A. lwoffii- and A. schindleri-specific PCR were 97.8 and 98.9%, respectively. The specificity of species-specific PCR for all other tested Acinetobacter species was 100%. The lower limit of detection for the nine species-specific PCR assays developed in this study was 20 or 200 pg of genomic DNA from type strains of each species. The Acinetobacter species-specific PCR assay would be useful to determine the correct species among suggested candidate Acinetobacter species when conventional methods including MALDI-TOF MS identify Acinetobacter only to the genus level. The species-specific assay can be used to screen large numbers of clinical and environmental samples obtained for epidemiologic study of Acinetobacter for the presence of target species.
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May 2016

Clostridium kogasensis sp. nov., a novel member of the genus Clostridium, isolated from soil under a corroded gas pipeline.

Anaerobe 2016 Jun 17;39:14-8. Epub 2016 Feb 17.

Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. Electronic address:

Two bacterial strains, YHK0403(T) and YHK0508, isolated from soil under a corroded gas pipe line, were revealed as Gram-negative, obligately anaerobic, spore-forming and mesophilic bacteria. The cells were rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates were members of the genus Clostridium and were the most closely related to Clostridium scatologenes KCTC 5588(T) (95.8% sequence similarity), followed by Clostridium magnum KCTC 15177(T) (95.8%), Clostridium drakei KCTC 5440(T) (95.7%) and Clostridium tyrobutyricum KCTC 5387(T) (94.9%). The G + C contents of the isolates were 29.6 mol%. Peptidoglycan in the cell wall was of the A1γ type with meso-diaminopimelic acid. The major polar lipid was diphosphatidylglycerol (DPG), and other minor lipids were revealed as phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unknown glycolipids (GL1 and GL2), an unknown aminoglycolipid (NGL), two unknown aminophospholipids (PN1 and PN2) and four unknown phospholipids (PL1 to PL4). Predominant fatty acids were C16:0 and C16:1cis9 DMA. The major end products from glucose fermentation were identified as butyrate (12.2 mmol) and acetate (9.8 mmol). Collectively, the results from a wide range of phenotypic tests, chemotaxonomic tests, and phylogenetic analysis indicated that the two isolates represent novel species of the genus Clostridium, for which the name Clostridium kogasensis sp. nov. (type strain, YHK0403(T) = KCTC 15258(T) = JCM 18719(T)) is proposed.
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http://dx.doi.org/10.1016/j.anaerobe.2016.02.006DOI Listing
June 2016

sp. nov., isolated from tree bark.

Int J Syst Evol Microbiol 2016 Mar 16;66(3):1187-1192. Epub 2015 Dec 16.

Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.

A Gram-stain-positive, facultatively anaerobic, rod-shaped and endospore-forming bacterium, strain BK114-2 isolated from tree bark in Thailand was characterized taxonomically using a polyphasic approach. Analysis based on comparison of 16S rRNA gene sequences indicated that strain BK114-2 was affiliated to the genus , and was closely related to 2301032 (96.7 % 16S rRNA gene sequence similarity), 3PO2SA (96.6 %) and SAFN-016 (96.4 %). Strain BK114-2 contained -diaminopimelic acid in its cell-wall peptidoglycan. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unknown phospholipids, five unknown aminophospholipids and six unknown lipids. The only menaquinone detected was MK-7 and the dominant cellular fatty acids were C (22.9 %), anteiso-C (22.6 %), iso-C (19.4 %) and anteiso-C (14.7 %). The DNA G+C content was 52.0 mol%. Based on these results, strain BK114-2 repreesents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BK114-2 ( = KCTC 33251 = TISTR 2282).
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http://dx.doi.org/10.1099/ijsem.0.000854DOI Listing
March 2016

Lactic Acid Bacterial Starter Culture with Antioxidant and γ-Aminobutyric Acid Biosynthetic Activities Isolated from Flatfish-Sikhae Fermentation.

J Med Food 2015 Dec 8;18(12):1371-9. Epub 2015 Sep 8.

1 Department of Food and Biotechnology, Korea University , Sejong, Korea.

The aim of this study is to select a lactic acid bacterial strain as a starter culture for flatfish-Sikhae fermentation and to evaluate its suitability for application in a food system. Four strains of lactic acid bacteria isolated from commercial flatfish-Sikhae were identified and selected as starter culture candidates through investigation of growth rates, salt tolerance, food safety, and functional properties such as antioxidative and antimicrobial activities. The fermentation properties of the starter candidates were also examined in food systems prepared with these strains (candidate batch) in comparison with a spontaneous fermentation process without starter culture (control batch) at 15°C. The results showed that the candidate YG331 batch had better fermentation properties such as viable cell count, pH, and acidity than the other experimental batches, including the control batch. The results are expressed according to selection criteria based on a preliminary sensory evaluation and physiochemical investigation. Also, only a small amount of histamine was detected with the candidate YG331 batch. The radical scavenging activity of the candidate batches was better compared with the control batch, and especially candidate YG331 batch showed the best radical scavenging activity. Also, we isolated another starter candidate (identified as Lactobacillus brevis PM03) with γ-aminobutyric acid (GABA)-producing activity from commercial flatfish-Sikhae products. The sensory scores of the candidate YG331 batch were better than those of the other experimental batches in terms of flavor, color, and overall acceptance. In this study, we established selection criteria for the lactic acid bacterial starter for the flatfish-Sikhae production and finally selected candidate YG331 as the most suitable starter.
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http://dx.doi.org/10.1089/jmf.2015.3458DOI Listing
December 2015

Eudoraea chungangensis sp. nov., isolated from an aquafarm waste water sludge.

Antonie Van Leeuwenhoek 2015 Apr 28;107(4):1009-15. Epub 2015 Jan 28.

Department of Microbiology, College of Medicine, Chung-Ang University (CAU), Seoul, South Korea.

A Gram-stain negative, non-spore-forming, non-motile, strictly aerobic bacterial strain, designated CAU 1296(T), was isolated from an aquafarm waste water sludge and its taxonomic position was investigated using a polyphasic approach. Cells of strain CAU 1296(T) grew optimally at 30 °C, at pH 7.5 and in the presence of 4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1296(T) formed a distinct lineage within the genus Eudoraea and exhibited similarity to Eudoraea adriatica AS06/20a(T) (95.4 % similarity). The major cellular fatty acids of the isolate were iso-C15:0, iso-C15:1 G, and summed feature 3 (C16:1 ω6c and C16:1 ω7c). The polar lipid pattern of strain CAU 1296(T) consisted of phosphatidylethanolamine, and unidentified lipids including a phosphoglycolipid, phospholipid, glycolipid, aminophospholipid, two aminolipids, and six further unidentified lipids. The strain contained menaquinone-6 (MK-6) as the major isoprenoid quinone. The G+C content of the genomic DNA was 38.7 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain CAU 1296(T) should be classified as a novel species in the genus Eudoraea, for which the name Eudoraea chungangensis sp. nov. is proposed. The type strain is CAU 1296(T) (=KCTC 42048(T), =CCUG 66239(T), =CECT 8744(T)).
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http://dx.doi.org/10.1007/s10482-015-0393-7DOI Listing
April 2015

Myroides injenensis sp. nov., a new member isolated from human urine.

Antonie Van Leeuwenhoek 2015 Jan 17;107(1):201-7. Epub 2014 Dec 17.

Korean Collection for Type Cultures, Biological Resource Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.

A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166(T)and M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis revealed that the isolates belong to the Myroides cluster and were closely related to Myroides phaeus DSM 23313(T) (96.3 %), Myroides odoratimimus KCTC 23053(T) (96.1 %), Myroides profundi KCTC 23066(T) (96.0 %), Myroides odoratus KCTC 23054(T) (95.4 %) and Myroides pelagicus KCTC 12661(T) (95.2 %). The major mena quinone was identified as MK-6. The major polar lipids were identified as phosphatidylethanolamine, amino lipids, and several unknown lipids, and the major fatty acids as iso-C15:0 and iso-C17:0 3-OH. Phenotypic and chemotaxonomic data supported the affiliation of the isolates with the genus Myroides and clearly indicated that two isolates represent novel species, for which the name Myroides injenensis sp. nov. (type strain, M09-0166(T) = KCTC 23367(T) = JCM 17451(T)) is proposed.
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http://dx.doi.org/10.1007/s10482-014-0317-yDOI Listing
January 2015

Peptoniphilus mikwangii sp. nov., isolated from a clinical specimen of human origin.

Curr Microbiol 2015 Feb 16;70(2):260-6. Epub 2014 Oct 16.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134(T), was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134(T) belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023(T) (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321(T) (93.8 %). The prevalent fatty acids of of strain ChDC B134(T) were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was D-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134(T) (=KCOM 1628(T) = KCTC 15227(T) = JCM 30223(T)) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-014-0712-7DOI Listing
February 2015

Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion.

Curr Microbiol 2015 Feb 26;70(2):169-75. Epub 2014 Sep 26.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

In this study, we classified the five strains (ChDC F128(T), ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA-DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA-DNA hybridization data showed that the values of genomic relatedness between ChDC F128(T) and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128(T) and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128(T) (=KCOM 1249(T) = KCTC 5108(T) = JCM 30218(T)) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00284-014-0692-7DOI Listing
February 2015