Publications by authors named "Youfen Qian"

6 Publications

  • Page 1 of 1

Biofilms on stone monuments: biodeterioration or bioprotection?

Trends Microbiol 2022 Sep 22;30(9):816-819. Epub 2022 Jun 22.

Environmental Science and Engineering Research Group, Guangdong Technion-Israel Institute of Technology (GTIIT), 241 Daxue Road, Shantou, Guangdong 515063, China; Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion, Guangdong Technion - Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong 515063, China. Electronic address:

Debate on whether biofilms on stone monuments are biodeteriorative or bioprotective is long-standing. We propose a criterion of 'relative bioprotective ratio' for assessing the ambivalent role of the biofilms by comparing biodeterioration with weathering. A boundary between biodeterioration and bioprotection exists and fluctuates with dynamic microflora influenced by environmental conditions.
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http://dx.doi.org/10.1016/j.tim.2022.05.012DOI Listing
September 2022

Innovative approaches for the processes involved in microbial biodeterioration of cultural heritage materials.

Curr Opin Biotechnol 2022 06 11;75:102716. Epub 2022 Apr 11.

Environmental Science and Engineering Research Group, Guangdong Technion-Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong 515063, China; Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion, Guangdong Technion-Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong 515063, China. Electronic address:

Microbial colonization and development into biofilms on cultural heritage have significant implications for the deterioration of materials, particularly in the tropic and humid environments. To advance the fundamental knowledge on the biofilm-mediated (bio)deterioration processes, future investigations must focus more on the metabolically active microorganisms and biochemical reactions by a combination of methods available. Newly accessible culture-independent techniques of high-throughput sequencing and multi-omics can be coupled with culture-dependent ones and specific biochemical assays, including stable isotopes and DNA probing. Here, we describe the recent advances on this subject matter, highlight a systematic analytical approach for an integrative diagnosis of 'microbial diseases' of cultural heritage, and provide future prospects for a new paradigm of research on microbial biodeterioration of heritage materials.
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http://dx.doi.org/10.1016/j.copbio.2022.102716DOI Listing
June 2022

[A polycyclic aromatic hydrocarbon degrading strain and its potential of degrading phenanthrene in various enhanced systems].

Sheng Wu Gong Cheng Xue Bao 2021 Oct;37(10):3696-3707

Jiangxi Province Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, China.

Polycyclic aromatic hydrocarbons (PAHs) are a class of common environmental pollutants that pose threats to human health. In this study, a mesophilic bacterial strain CFP312 (grown at 15-37 °C, optimal at 30 °C) was isolated from PAHs-contaminated soil samples. It was identified as Moraxella sp. by morphological observation, physiological and biochemical test, and 16S rRNA gene phylogeny analysis. This is the first reported PAHs degrading strains in Moraxella. Degradation analysis showed that 84% and 90% of the loaded phenanthrene (400 mg/L) were degraded within 48 h and 60 h, and the degradation rates reached 1.21 and 1.29 mg/(L·h), respectively. During the degradation of phenanthrene, phenanthrene-3,4-dihydrodiol was detected as an intermediate. Based on this, it was proposed that double oxygenation at the positions 3 and 4 of phenanthrene was the first step of biodegradation. Adaptability of strain CFP312 to different enhanced phenanthrene-degradation systems was tested in aqueous-organic system, micellar aqueous system, and cloud point system. Strain CFP312 showed good adaptability to different systems. In addition, the bacterium can rapidly degrade the phenanthrene in contaminated soil in slurry-aqueous system, indicating great potential in environmental remediation.
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http://dx.doi.org/10.13345/j.cjb.210212DOI Listing
October 2021

Synergistic interactions of Desulfovibrio and Petrimonas for sulfate-reduction coupling polycyclic aromatic hydrocarbon degradation.

J Hazard Mater 2021 04 4;407:124385. Epub 2020 Nov 4.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

Microbial sulfate-reduction coupling polycyclic aromatic hydrocarbon (PAH) degradation is an important process for the remediation of contaminated sediments. However, little is known about core players and their mechanisms in this process due to the complexity of PAH degradation and the large number of microorganisms involved. Here we analyzed potential core players in a black-odorous sediment using gradient-dilution culturing, isolation and genomic/metagenomic approaches. Along the dilution gradient, microbial PAH degradation and sulfate consumption were not decreased, and even a significant (p = 0.003) increase was observed in the degradation of phenanthrene although the microbial diversity declined. Two species, affiliated with Desulfovibrio and Petrimonas, were commonly present in all of the gradients as keystone taxa and showed as the dominant microorganisms in the single colony (SB8) isolated from the highest dilution culture with 93.49% and 4.73% of the microbial community, respectively. Desulfovibrio sp. SB8 and Petrimonas sp. SB8 could serve together as core players for sulfate-reduction coupling PAH degradation, in which Desulfovibrio sp. SB8 could degrade PAHs to hexahydro-2-naphthoyl through the carboxylation pathway while Petrimonas sp. SB8 might degrade intermediate metabolites of PAHs. This study provides new insights into the microbial sulfate-reduction coupling PAH degradation in black-odorous sediments.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124385DOI Listing
April 2021

sp. nov., isolated from freshwater sediment.

Int J Syst Evol Microbiol 2020 Oct 20;70(10):5205-5210. Epub 2020 Aug 20.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.

A Gram stain-positive, non-spore-forming, non-motile and rod-shaped actinomycete, strain 5221, was isolated from the sediment of a river collected at Ronggui in the Pearl River Delta, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a distinct lineage within the genus and had the highest sequence similarity to Tp12 (96.7 %), followed by 2C6-41 (96.5 %), SST-8 (96.0 %) and 20 (95.9 %). The results of chemotaxonomic analyses, including detecting anteiso-C, anteiso-C, and C as the major cellular fatty acids, diphosphatidylglycerol, phosphatidylglycerol and three phosphoglycolipids as the polar lipids, MK-8(H) as the major menaquinone, and a DNA G+C content of 72.4 mol%, supported that strain 5221 is a member of the genus . Furthermore, low sequence similarities of 16S rRNA gene sequences, differences in fatty acid compositions and differential physiological characteristics such as enzyme activity and carbon sources utilization ability distinguished the isolate from its close relatives. Therefore, strain 5221 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain 5221 (=GDMCC 1.1766=KACC 21700).
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http://dx.doi.org/10.1099/ijsem.0.004379DOI Listing
October 2020

Ciceribacter ferrooxidans sp. nov., a nitrate-reducing Fe(II)-oxidizing bacterium isolated from ferrous ion-rich sediment.

J Microbiol 2020 May 27;58(5):350-356. Epub 2020 Apr 27.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, P. R. China.

A nitrate-reducing Fe(II)-oxidizing bacterial strain, F8825, was isolated from the Fe(II)-rich sediment of an urban creek in Pearl River Delta, China. The strain was Gram-negative, facultative chemolithotrophic, facultative anaerobic, non-spore-forming, and rod-shaped with a single flagellum. Phy-logenetic analysis based on 16S rRNA gene sequencing indicated that it belongs to the genus Ciceribacter and is most closely related to C. lividus MSSRFBL1 (99.4%), followed by C. thiooxidans F43b (98.8%) and C. azotifigens A.slu09 (98.0%). Fatty acid, polar lipid, respiratory quinone, and DNA G + C content analyses supported its classification in the genus Ciceribacter. Multilocus sequence analysis of concatenated 16S rRNA, atpD, glnII, gyrB, recA, and thrC suggested that the isolate was a novel species. DNA-DNA hybridization and genome sequence comparisons (90.88 and 89.86%, for values of ANIm and ANIb between strains F8825 with MSSRFBL1, respectively) confirmed that strain F8825 was a novel species, different from C. lividus MSSRFBL1, C. thiooxidans F43b, and C. azotifigens A.slu09. The physiological and biochemical properties of the strain, such as carbon source utilization, nitrate reduction, and ferrous ion oxidation, further supported that this is a novel species. Based on the polyphasic taxonomic results, strain F8825 was identified as a novel species in the genus Ciceribacter, for which the name Ciceribacter ferrooxidans sp. nov. is proposed. The type strain is F8825 (= CCTCC AB 2018196 = KCTC 62948).
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http://dx.doi.org/10.1007/s12275-020-9471-2DOI Listing
May 2020
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