Publications by authors named "You Wu"

197 Publications

Accelerating trajectory manipulation of symmetric Pearcey Gaussian beam in a uniformly moving parabolic potential.

Opt Express 2021 May;29(11):16270-16283

We derive analytical solutions that describe the one-dimensional displaced and chirped symmetric Pearcey Gaussian beam in a uniformly moving parabolic potential. The multiple effective manipulations of the beam, which are originated from the diverse configurations of the dynamic parabolic potential, are demonstrated. On the whole, the accelerating trajectory can transform into a linear superposition form of the oblique straight line and the simple harmonic motion. Meanwhile, we discuss the further modulation of the accelerating trajectory characteristics such as slope, amplitude and phase shift. Additionally, the extension into a two-dimensional scenario is also proposed. Our results theoretically improve the practical value of the Pearcey beam, and lead to potential applications in trajectory manipulation and particle manipulation.
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http://dx.doi.org/10.1364/OE.424489DOI Listing
May 2021

Chitosan-sodium alginate-based coatings for self-strengthening anticorrosion and antibacterial protection of titanium substrate in artificial saliva.

Int J Biol Macromol 2021 Jun 10;184:109-117. Epub 2021 Jun 10.

Key Laboratory for Colloid and Interface Chemistry of State Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. Electronic address:

A self-strengthening coating with silver nanoparticles (Ag NPs) doped chitosan (CHI) and sodium alginate (SA) polyelectrolytes was constructed on the surface of polydopamine (PDA) coated Ti substrate by a layer-by-layer assembly method. The PDA coating exhibited an excellent bond with Ti substrate, and also can uniformly deposit Ag NPs via a mild method without introducing any exogenous reductant. The CHI coating was assembled through a spin-coating method for controlling Ag release. The SA was introduced to enhance the anticorrosion performance by forming calcium alginate (CA) in a corrosive medium. The corrosion protection was investigated with electrochemical impedance spectroscopy and polarization curves tests in fluorine-containing artificial saliva. During immersion, the charge-transfer resistance and the protection efficiency (ŋ) presented a continuous increase with the immersion time, demonstrating that this coating possessed a remarkable self-strengthening capability, and the compositions of the outermost film changed from SA to CA with the Ca cations of the corrosive medium as a crosslinker by SEM and EDS analysis. Furthermore, the ŋ remained up to 96.8% after immersion of 30 days, and then the coating also displayed a distinct inhibition zone on S. mutans. These results prove this coating possesses an excellent anticorrosion performance and antibacterial property.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.042DOI Listing
June 2021

Genomic sequencing for the diagnosis of childhood mitochondrial disorders: a health economic evaluation.

Eur J Hum Genet 2021 Jun 8. Epub 2021 Jun 8.

Health Economics Unit, Centre for Health Policy, Melbourne School of Population and Global Health, University of Melbourne, Melbourne, VIC, Australia.

The diagnostic and clinical benefits of genomic sequencing are being increasingly demonstrated across multiple rare genetic conditions. Despite the expanding clinical literature, there is a significant paucity of health economics evidence to inform the prioritization and implementation of genomic sequencing. This study aims to evaluate whether genomic sequencing for pediatric-onset mitochondrial disorders (MDs) is cost-effective and cost-beneficial relative to conventional care from an Australian healthcare system perspective. Two independent and complementary health economic modeling approaches were used. Approach 1 used a decision tree to model the costs and outcomes associated with genomic sequencing and conventional care. Approach 2 used a discrete-event simulation to incorporate heterogeneity in the condition and clinical practice. Deterministic and probabilistic sensitivity analyses were performed. Genomic sequencing was less costly and more effective compared with conventional care, saving AU$1997 (Approach 1) to AU$8823 (Approach 2) per child tested, while leading to an additional 11 (Approach 1) to 14 (Approach 2) definitive diagnoses per 100 children tested. The mean monetary value of the incremental benefits of genomic sequencing was estimated at AU$5890 (95% CI: AU$5730-$6046). Implementation of genomic sequencing for MDs in Australia could translate to an annual cost-saving of up to AU$0.7 million. Genomic sequencing is cost-saving relative to traditional investigative approaches, while enabling more diagnoses to be made in a timely manner, offering substantial personal benefits to children and their families. Our findings support the prioritization of genomic sequencing for children with MDs.
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http://dx.doi.org/10.1038/s41431-021-00916-8DOI Listing
June 2021

Blending urea and slow-release nitrogen fertilizer increases dryland maize yield and nitrogen use efficiency while mitigating ammonia volatilization.

Sci Total Environ 2021 May 28;790:148058. Epub 2021 May 28.

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of the Ministry of Education, Northwest A&F University, Yangling, Shaanxi 712100, China.

Agricultural non-point source pollution has become the main pollution source in China. Ammonia (NH) volatilization is one of the main factors of agricultural non-point source pollution. Slow-release nitrogen fertilizer (S) has been widely recognized as an efficient management measure to increase crop yields and mitigate NH volatilization. However, few studies have reported the effects of urea (U) blended with slow-release nitrogen fertilizer (UNS) on maize yield and NH volatilization under dryland farming conditions. A two-season field experiment with U, S and various blending ratios of U and S (UNS) under two N application rates (N1: 180 kg N ha, N2: 240 kg N ha) was conducted to determine their effects on maize yield, NH volatilization and residual soil NO-N. The results showed that UNS substantially reduced NH volatilization compared with U, primarily because of the relatively low soil pH and electrical conductivity, and the relatively high soil organic matter. UNS significantly increased dry matter, grain yield, N uptake and N use efficiency (NUE), but reduced residual soil NO-N compared with U and S. Among UNS treatments, the blending ratio of U and S at 3:7 (UNS2) was most effective in improving maize yield and NUE, while mitigating NH volatilization and soil NO-N leaching. N1 not only reduced N losses, but also increased NUE compared with N2. In conclusion, UNS2N1 is recommended as the best N fertilizer application strategy for the sustainable production of dryland maize in northwest China.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148058DOI Listing
May 2021

Nanoparticle-mediated surfactant therapy in patients with severe COVID-19: a perspective.

J Mater Chem B 2021 Jun 4. Epub 2021 Jun 4.

Department of Chemical and Biological Engineering, The University of Alabama, P. O. Box 870203, Tuscaloosa, AL 35401, USA.

Coronavirus disease 2019 (COVID-19) is an RNA virus-based disease that can be deadly. For critically ill patients, mechanical ventilation is an important life-saving treatment. However, mechanical ventilation shows a trade-off between supporting respiratory function and ventilator-induced lung injury (VILI). Surfactant therapy is a medical administration of exogenous surfactant to supplement or replace deficient or dysfunctional endogenous surfactant. Surfactant therapy can be used to postpone or shorten the use of mechanical ventilation to minimize or avoid VILI, because surfactants can reduce surface tension, improve lung compliance, and enhance oxygenation. In addition, nanotechnology can be applied to improve the therapeutic effect and reduce the adverse effects of surfactants. In this perspective, we discussed how nanoparticles deliver surfactants through intravenous injection and inhalation to the expected lung disease regions where surfactants are mostly needed, and discussed the prospects of nanoparticle-mediated surfactant therapy in the treatment of patients with severe COVID-19.
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http://dx.doi.org/10.1039/d1tb00730kDOI Listing
June 2021

Symmetric Pearcey Gaussian beams.

Opt Lett 2021 May;46(10):2461-2464

In this Letter, a new, to the best of our knowledge, type of autofocusing and symmetric beam arisen from two quartic spectral phases is introduced in theory and experiment. The symmetric Pearcey Gaussian beam (SPGB), formed with a Gaussian term and two multiplying Pearcey integrals, processes a focusing intensity approximately 1.32 times stronger than the intensity of the symmetric Airy beam. Its four off-axis main lobes split into four bending trajectories symmetrically after focusing. The rectangular intensity distribution and the focal length of the SPGB can be adjusted by two kinds of distribution factors. Additionally, the vortex-guiding property of the beam is demonstrated by embedding an off-axis vortex into the SPGB, which can be applied in particle guiding.
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http://dx.doi.org/10.1364/OL.425889DOI Listing
May 2021

Preparation of GO/MIL-101(Fe,Cu) composite and its adsorption mechanisms for phosphate in aqueous solution.

Environ Sci Pollut Res Int 2021 May 13. Epub 2021 May 13.

College of Geology and Environment, Xi'an University of science and technology, Xi'an, 710054, People's Republic of China.

In this study, MIL-101(Fe), MIL-101(Fe,Cu), and graphene oxide (GO)/MIL-101(Fe,Cu) were synthesized to compose a novel sorbent. The adsorption properties of these three MOF-based composites were compared toward the removal of phosphate. Furthermore, the influencing factors including adsorption time, pH, temperature, and initial concentration on the adsorption capacity of phosphate on these materials as well as the reusability of the material were discussed. The structure of fabricated materials and the removal mechanism of phosphate on the composite material were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), nitrogen adsorption-desorption analysis, and zeta potential. The results show that the maximum adsorption capacity of phosphate by the composite GO/MIL-101(Fe,Cu)-2% was 204.60 mg·g, which is higher than that of MIL-101(Fe,Cu) and MIL-101(Fe). likewise the specific surface area of GO/MIL-101(Fe,Cu)-2% is 778.11 m/g is higher than that of MIL-101(Fe,Cuand MIL-101(Fe),which are 747.75 and 510.66 m/g, respectively. The adsorption mechanism of phosphate is electrostatic attraction, forming coordination bonds and hydrogen bonds. The fabricated material is a promising adsorbent for the removal of phosphate with good reusability.
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http://dx.doi.org/10.1007/s11356-021-14206-9DOI Listing
May 2021

Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system.

J Zhejiang Univ Sci B 2021 May;22(5):421-430

College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively. The treatment was discontinued after two weeks, and the spinal cord was obtained for hematoxylin and eosin (H&E) and luxol fast blue staining, as well as retinoic acid receptor-related orphan receptor γ (RORγ) and forkhead box protein P3 (Foxp3) immunohistochemical staining. Blood was collected for Th17 and Treg cell flow cytometry testing, and the serum levels of interleukin (IL)-17A, IL-10, transforming growth factor-β (TGF-β), IL-6, IL-23, and IL-2 were measured via enzyme-linked immunosorbent assay (ELISA). Compared with rats in the EAE group, rats in the EAE+cornuside and EAE+prednisolone groups began to recover from neurological deficits earlier, and had a greater degree of improvement of symptoms. Focal inflammation, demyelination, and RORγ-positive cell infiltration were reduced by cornuside or prednisolone treatment, whereas the Foxp3-positive cell numbers were not significantly different. Meanwhile, the number of Th17 cells and the IL-17A, IL-6, and IL-23 levels were lower in the blood after cornuside or prednisolone treatment, whereas the number of Treg cells or the levels of IL-10, TGF-β, and IL-2 were not markedly different. Cornuside can alleviate symptoms of EAE neurological deficits through its anti-inflammatory and immunosuppressive effects, and Th17 cells may be one of its therapeutic targets.
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http://dx.doi.org/10.1631/jzus.B2000771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110462PMC
May 2021

Dairy foods, calcium, and risk of breast cancer overall and for subtypes defined by estrogen receptor status: a pooled analysis of 21 cohort studies.

Am J Clin Nutr 2021 May 8. Epub 2021 May 8.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, MD, USA.

Background: Epidemiologic studies examining the relations between dairy product and calcium intakes and breast cancer have been inconclusive, especially for tumor subtypes.

Objective: To evaluate the associations between intakes of specific dairy products and calcium and risk of breast cancer overall and for subtypes defined by estrogen receptor (ER) status.

Method: We pooled the individual-level data of over 1 million women who were followed for a maximum of 8-20 years across studies. Associations were evaluated for dairy product and calcium intakes and risk of incident invasive breast cancer overall (n = 37,861 cases) and by subtypes defined by ER status. Study-specific multivariable hazard ratios (HRs) were estimated and then combined using random-effects models.

Results: Overall, no clear association was observed between the consumption of specific dairy foods, dietary (from foods only) calcium, and total (from foods and supplements) calcium, and risk of overall breast cancer. Although each dairy product showed a null or very weak inverse association with risk of overall breast cancer (P, test for trend >0.05 for all), differences by ER status were suggested for yogurt and cottage/ricotta cheese with associations observed for ER-negative tumors only (pooled HR = 0.90, 95% CI: 0.83, 0.98 comparing ≥60 g/d with <1 g/d of yogurt and 0.85, 95% CI: 0.76, 0.95 comparing ≥25 g/d with <1 g/d of cottage/ricotta cheese). Dietary calcium intake was only weakly associated with breast cancer risk (pooled HR = 0.98, 95% CI: 0.97, 0.99 per 350 mg/d).

Conclusion: Our study shows that adult dairy or calcium consumption is unlikely to associate with a higher risk of breast cancer and that higher yogurt and cottage/ricotta cheese intakes were inversely associated with the risk of ER-negative breast cancer, a less hormonally dependent subtype with poor prognosis. Future studies on fermented dairy products, earlier life exposures, ER-negative breast cancer, and different racial/ethnic populations may further elucidate the relation.
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http://dx.doi.org/10.1093/ajcn/nqab097DOI Listing
May 2021

A signalling cascade for Ral.

Small GTPases 2021 May 6:1-8. Epub 2021 May 6.

Texas A&M University, Houston, TX, USA.

Ras is the most mutated oncoprotein in cancer. Among the three oncogenic effectors of Ras - Raf, PI3 Kinase and RalGEF>Ral - signalling through RalGEF>Ral (Ras-like) is by far the least well understood. A variety of signals and binding partners have been defined for Ral, yet we know little of how Ral functions . This review focuses on previous research in that defined a function for Ral in apoptosis and established indirect relationships among Ral, the CNH-domain MAP4 Kinase , and the JNK MAP kinase . Most of the described signalling components are not essential in , facilitating subsequent analysis using developmental patterning of the vulval precursor cells (VPCs). The functions of two paralogous CNH-domain MAP4 Kinases were defined relative to Ras>Raf, Notch and Ras>RalGEF>Ral signalling in VPCs. MIG-15, the nematode ortholog of , antagonizes both the Ral-dependent and Ras>Raf-dependent developmental outcomes. In contrast, paralogous GCK-2, the ortholog of , propagates the 2°-promoting signal of Ral. Manipulations via CRISPR of Ral signalling through GCK-2 coupled with genetic epistasis delineated a Ras>RalGEF>Ral>Exo84>GCK-2>MAP3K> p38 cascade. Thus, genetic analysis using invertebrate experimental organisms defined a cascade from Ras to p38 MAP kinase.
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http://dx.doi.org/10.1080/21541248.2021.1917953DOI Listing
May 2021

Establishment of a prognostic model of four genes in gastric cancer based on multiple data sets.

Cancer Med 2021 05 2;10(10):3309-3322. Epub 2021 May 2.

Department of General Surgery, Nanchang University, The Second Affiliated Hospital of Nanchang University, NanChang, JiangXi, 330006, China.

Gastric cancer (GC) is a kind of malignancy with a high mortality and recurrence. An effective prediction model based on ideal biomarkers to assess prognosis could benefit patients for optimization of treatment. Bioinformatics has played an increasingly important role in the study of cancer diseases. Therefore, this study started with bioinformatics to establish a reliable prognostic model of gastric cancer. The gene expression data and clinical data of GC tissues and normal tissues were obtained from the Gene Expression Omnibus (GEO), Genotype-Tissue Expression (GTEx), and The Cancer Genome Atlas (TCGA) profile database. We finally identified a four gene signature and constructed a prognostic model. The results of internal and external validation showed that the model is highly reliable. In addition, we also constructed a nomogram based on the model, which was verified by a calibration curve to show its predicted accuracy. Comprehensive analysis indicated that the four genes in the model are related to the occurrence and development of tumors, perhaps they are potential targets for tumor treatment. Generally, this prognostic model can bring potential benefits to patients with gastric cancer.
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http://dx.doi.org/10.1002/cam4.3654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124107PMC
May 2021

Nuclear mA reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

Protein Cell 2021 Jun 22;12(6):455-474. Epub 2021 Apr 22.

Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, 200120, China.

N-methyladenosine (mA) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but the intrinsic mechanism remains unclear. Here, we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells (ESCs), which highly depends on the mA-binding ability. Ythdc1 is required for sufficient rRNA synthesis and repression of the 2-cell (2C) transcriptional program in ESCs, which recapitulates the transcriptome regulation by the LINE1 scaffold. Detailed analyses revealed that YTHDC1 recognizes mA on LINE1 RNAs in the nucleus and regulates the formation of the LINE1-NCL partnership and the chromatin recruitment of KAP1. Moreover, the establishment of H3K9me3 on 2C-related retrotransposons is interrupted in Ythdc1-depleted ESCs and inner cell mass (ICM) cells, which consequently increases the transcriptional activities. Our study reveals a role of mA in regulating the RNA scaffold, providing a new model for the RNA-chromatin cross-talk.
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http://dx.doi.org/10.1007/s13238-021-00837-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160034PMC
June 2021

Network Pharmacology Analysis of ZiShenWan for Diabetic Nephropathy and Experimental Verification of Its Anti-Inflammatory Mechanism.

Drug Des Devel Ther 2021 15;15:1577-1594. Epub 2021 Apr 15.

Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.

Background: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD). The inflammatory response plays a critical role in DN. ZiShenWan (ZSW) is a classical Chinese medicinal formula with remarkable clinical therapeutic effects on DN, but its pharmacological action mechanisms remain unclear.

Aim: In this study, a network pharmacology approach was applied to investigate the pharmacological mechanisms of ZSW in DN therapy. Based on the results of network analysis, the core targets and signaling pathways related to anti-inflammatory effect were verified via experiments in vivo.

Methods: The candidate chemical ingredients of ZSW as well as its putative targets and known therapeutic targets of DN were acquired from appropriate databases. The "herb-ingredient-target" network for ZSW in DN treatment was established. The protein-protein interaction (PPI) network of potential targets was constructed to screen the core targets. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. In addition to biochemical and pathological indicators, the core targets and signaling pathways associated with inflammation were partially validated in db/db mice at molecular level.

Results: A total of 56 active ingredients in ZSW and 166 DN-related targets were selected from databases. A high proportion of core targets and top signaling pathways participate in inflammation. ZSW markedly alleviated renal injuries pathologically and regulated related biomarkers. In particular, ZSW significantly inhibited the exaggerated release of inflammatory cytokines such as interleukin (IL)-1β, IL-6, tumor necrosis factor receptor (TNF)-ɑ, and monocyte chemotactic protein (MCP)-1 as well as regulating p38 mitogen-activated protein kinases (MAPK) and phosphoinositide 3-kinase (PI3K)-protein kinase B (Akt) signaling pathways in db/db mice.

Conclusion: This study first comprehensively investigated the active ingredients, potential targets, and molecular mechanism of ZSW as a therapy for DN. ZSW achieved renoprotective effects in DN via regulation of multiple targets and signaling pathways, especially by alleviating inflammation. Results indicate that ZSW is a promising multi-target therapeutic approach for DN treatment.
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http://dx.doi.org/10.2147/DDDT.S297683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055297PMC
April 2021

Further Results on Adaptive Practical Tracking for High-Order Nonlinear Systems With Full-State Constraints.

IEEE Trans Cybern 2021 Apr 20;PP. Epub 2021 Apr 20.

In this article, an adaptive practical tracking control scheme is presented for full-state constrained high-order nonlinear systems. By skillfully introducing the adaptive gain, nonlinear transformed functions and sign functions into control design, a novel continuous state-feedback controller is constructed without imposing restrictive approximation techniques and feasibility conditions. Under mild assumptions, the boundedness of all the closed-loop signals can be guaranteed, full-state constraints are not transgressed for all time, and the tracking error tends to an arbitrarily small region of zero in a finite time.
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http://dx.doi.org/10.1109/TCYB.2021.3069865DOI Listing
April 2021

A Novel Pyramid Network with Feature Fusion and Disentanglement for Object Detection.

Comput Intell Neurosci 2021 15;2021:6685954. Epub 2021 Mar 15.

School of Computer Science, South China Normal University, Guangzhou 510631, China.

In order to alleviate the scale variation problem in object detection, many feature pyramid networks are developed. In this paper, we rethink the issues existing in current methods and design a more effective module for feature fusion, called multiflow feature fusion module (MFM). We first construct gate modules and multiple information flows in MFM to avoid information redundancy and enhance the completeness and accuracy of information transfer between feature maps. Furtherore, in order to reduce the discrepancy of classification and regression in object detection, a modified deformable convolution which is termed task adaptive convolution (TaConv) is proposed in this study. Different offsets and masks are predicted to achieve the disentanglement of features for classification and regression in TaConv. By integrating the above two designs, we build a novel feature pyramid network with feature fusion and disentanglement (FFAD) which can mitigate the scale misalignment and task misalignment simultaneously. Experimental results show that FFAD can boost the performance in most models.
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http://dx.doi.org/10.1155/2021/6685954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987438PMC
March 2021

Association of folate intake and colorectal cancer risk in the postfortification era in US women.

Am J Clin Nutr 2021 Mar 19. Epub 2021 Mar 19.

Department of Nutrition, Harvard TH Chan School of Public Health, Boston, MA, USA.

Background: Folate may play a preventive role in the early stages of colorectal carcinogenesis, but long latencies may be needed to observe a reduction in colorectal cancer (CRC) incidence. In addition, concerns have been raised about the potential for cancer promotion with excessive folate intake, especially after the mandatory folic acid fortification in the United States in 1998.

Objective: We aimed to examine the association between folate intake in different chemical forms and CRC risk, especially in the postfortification era in the United States.

Design: We prospectively followed 86,320 women from the Nurses' Health Study (1980-2016). Folate intake was collected by validated food frequency questionnaires. CRC was self reported and confirmed by review of medical records. The association between the folate intake and CRC risk was assessed using Cox proportional hazards regression.

Results: We documented 1988 incident CRC cases during follow-up. Analyzing folate intake as a continuous variable, greater total folate intake 12-24 y before diagnosis was associated with lower risk of CRC (per increment of 400 dietary folate equivalents (DFE)/d, HR: 0.93, 95% CI: 0.85, 1.01 for 12-16 y; HR: 0.83, 95% CI: 0.75, 0.92 for 16-20 y; and HR: 0.87, 95% CI: 0.77, 0.99 for 20-24 y); and greater synthetic folic acid intake 16-24 y before diagnosis was also associated with a lower CRC risk (per increment of 400 DFE/d, HR: 0.91, 95% CI: 0.84, 0.99 for 16-20 y and HR: 0.91, 95% CI: 0.83-1.01 for 20-24 y). In the postfortification period (1998-2016), intake of total or specific forms of folate was not associated with CRC risk, even among multivitamin users.

Conclusions: Folate intake, both total and from synthetic forms, was associated with a lower risk of overall CRC after long latency periods. There was no evidence that high folate intake in the postfortification period was related to increased CRC risk in this US female population.
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http://dx.doi.org/10.1093/ajcn/nqab035DOI Listing
March 2021

Tonalide facilitates methane production from anaerobic digestion of waste activated sludge.

Sci Total Environ 2021 Jul 3;779:146195. Epub 2021 Mar 3.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University, Ministry of Education, Changsha 410082, PR China. Electronic address:

Tonalide (AHTN), a typical polycyclic musk and an emerging pollutant, was found to be enriched in waste activated sludge (WAS). However, the research of its effect on WAS anaerobic digestion was seldom available. This research therefore investigated the effect of AHTN on WAS anaerobic digestion and the underlying mechanism through batch experiments using either real WAS or synthetic wastewaters as the digestion substrates. The results indicated that when the concentration of AHTN increased from 0 to 1000 mg/kg TSS in WAS, the methane production increased linearly from 125.0 ± 2.2 to 162.9 ± 1.6 mL/g VSS, while the AHTN concentration further increased to 2000 mg/kg TSS, the methane production decreased to 146.2 ± 2.1 mL/g VSS. At the same time AHTN can facilitate the utility of volatile fatty acid (VFAs), especially acetate and propionate. It was further found that the degradation efficiency of AHTN in anaerobic digestion was 42.7%. The mechanism investigation demonstrated that AHTN can promote the solubilization, homoacetogenesis, acetogenesis and methanogenesis processes, leading to an increase in methane production. Further analysis revealed that methanogenic archaea mainly belonged to the genera of Methanosaeta and Metheanobacterium, and their relative abundance increased accordingly with the addition of AHTN.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146195DOI Listing
July 2021

Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model.

Sci Rep 2021 Mar 18;11(1):6401. Epub 2021 Mar 18.

Key Laboratory of TCM Health Cultivation of Beijing, Beijing University of Chinese Medicine, Beijing, China.

In a mouse model of Graves' disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protective effect of diosgenin against AIT in rats and the possible mechanism. The results showed that in the diosgenin-intervention group, compared to the model group, the expression of serum triiodothyronine, thyroxine, free triiodothyronine, and free thyroxine was decreased and that of thyroid-stimulating hormone was increased; these changes were accompanied by the downregulation of thyroglobulin, TSH receptor antibody and thyroid peroxidase expression in serum. Furthermore, transcriptome detection, RT-qPCR and immunohistochemistry verification revealed that in thyroid tissue, the relative mRNA and protein expression of cyclic adenosine 3',5'-monophosphate (cAMP), protein kinase A (PKA) and cAMP response element-binding protein (Creb) were increased and the mRNA expression of S100 calcium-binding protein A9 (S100A9) was decreased in the diosgenin groups. In summary, diosgenin alleviates the development of AIT, possibly via the activation of the cAMP/PKA/Creb pathway and downregulation of S100A9 gene expression.
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http://dx.doi.org/10.1038/s41598-021-85822-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973441PMC
March 2021

Substrate-Tuned Domino Annulation for Selective Synthesis of Poly-substituted Benzo[]imidazo[2,1-][2,7]naphthyridines and 3-Azaheterocyclic Substituted 2-Arylquinolines.

J Org Chem 2021 Mar 25;86(6):4747-4755. Epub 2021 Feb 25.

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People's Republic of China, 310058.

A domino annulation/oxidation of heterocyclic ketene aminals (HKAs) and 2-aminochalcones has been developed for the selective synthesis of poly-substituted benzo[]imidazo[2,1-][2,7]naphthyridines and 3-azaheterocyclic substituted 2-arylquinolines. These reactions proceed well under mild conditions without any additives. Plausible mechanisms for such a polycyclic ring system assembly were also proposed. Moreover, benzo[]imidazo[2,1-][2,7]naphthyridine displayed a fluorescence effect, demonstrating the potential applications in organic optical materials.
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http://dx.doi.org/10.1021/acs.joc.1c00112DOI Listing
March 2021

Oral administration of mangiferin ameliorates diabetes in animal models: a meta-analysis and systematic review.

Nutr Res 2021 03 25;87:57-69. Epub 2020 Dec 25.

Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing, 100029, China. Electronic address:

Although mangiferin has a number of documented beneficial effects, there are no systematic reviews or meta-analyses of its effects in diabetic animal models. To investigate the effects of oral administration of mangiferin on blood glucose levels, body weight, and total cholesterol and triglycerides levels in diabetic animal models, a meta-analysis was conducted and the underlying mechanisms were reviewed. Studies from 6 databases (PubMed, Web of Science, Embase, Cochrane Library, and CNKI (China National Knowledge Infrastructure), and Wanfang Med) were searched from inception to April 2020. After article screening, a total of 19 articles were included in this meta-analysis. The meta-analysis was performed using RevMan 5.3 and STATA 14.0 software. The overall pooled estimate of standardized mean difference (SMD) of mangiferin's effect on blood glucose was -1.27 (95% confidence interval [CI]: -1.71, -0.82, P < .00001). Body weight increased in lean diabetic animals with an SMD of 1.41 (95% CI: 0.57, 2.25; P = .001), while it decreased in obese diabetic animals with an SMD of -0.92 (95% CI: -1.69, -0.14; P = .02). Mangiferin intake reduced serum total cholesterol and triglycerides levels with SMDs of -1.02 (95% CI: -1.43, -0.61; P < .001) and -1.24 (95% CI: -1.70, -0.79; P < .001), respectively. The meta-analysis suggests that oral intake of mangiferin has a significant antidiabetic effect in animal models, and the systematic review suggested that this function might be attributed to its anti-inflammatory and antioxidative properties, as well as to its function of improving glycolipid metabolism and enhancing insulin signaling.
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http://dx.doi.org/10.1016/j.nutres.2020.12.017DOI Listing
March 2021

Ureteral polyps protruding from the urethra.

BMC Urol 2021 Feb 10;21(1):22. Epub 2021 Feb 10.

Department of Urology, Affiliated Hospital of Nantong University, No. 20 Xisi Road, Nantong, China.

Background: Ureteral fibro-epithelial polyp (UFP) is a rare benign ureteral tumor, and surgical removal of the polyps is still the preferred solution. Although many cases have reported polyps extending to the bladder, our case was the first to report a huge UFP that underwent endoscopic laser resection to highlight the urethra and cause severe end hematuria permanently.

Case Presentation: In 2019, a 37-year-old woman came to the hospital because of hematuria and a dark red extraurethral mass. CTU inspection showed: filling defect between the right ureter and the bladder at the entrance of the bladder. After ureteroscopy, it was found that the ureteral mass came out of the urethral orifice. Then, under the direct view of the ureteroscope, a Ho:YAG laser was used to remove the tumor by cutting off along the its base, and the patient was discharged 3 days after the operation.

Conclusion: Urethral polyps from the ureter should be considered in the differential diagnosis of urethral neoplasms. Ho:YAG laser resection under ureteroscopy is an effective option for treating UFP, but be careful of ureteral stricture after surgery.
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http://dx.doi.org/10.1186/s12894-021-00786-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877100PMC
February 2021

HMGB1 regulates ferroptosis through Nrf2 pathway in mesangial cells in response to high glucose.

Biosci Rep 2021 Feb;41(2)

Department of Endocrinology, The Second Hospital of Jilin University, Changchun 130041, China.

Ferroptosis, a novel type of programmed cell death, is involved in inflammation and oxidation of various human diseases, including diabetic kidney disease. The present study explored the role of high-mobility group box-1 (HMGB1) on the regulation of ferroptosis in mesangial cells in response to high glucose. Compared with healthy control, levels of serum ferritin, lactate dehydrogenase (LDH), reactive oxygen species (ROS), malonaldehyde (MDA), and HMGB1 were significantly elevated in diabetic nephropathy (DN) patients, accompanied with deregulated ferroptosis-related molecules, including long-chain acyl-CoA synthetase 4 (ACSL4), prostaglandin-endoperoxide synthase 2 (PTGS2), NADPH oxidase 1 (NOX1), and glutathione peroxidase 4 (GPX4). In vitro assay revealed that erastin and high glucose both induced ferroptosis in mesangial cells. Suppression of HMGB1 restored cellular proliferation, prevented ROS and LDH generation, decreased ACSL4, PTGS2, and NOX1, and increased GPX4 levels in mesangial cells. Furthermore, nuclear factor E2-related factor 2 (Nrf2) was decreased in DN patients and high glucose-mediated translocation of HMGB1 in mesangial cells. Knockdown of HMGB1 suppressed high glucose-induced activation of TLR4/NF-κB axis and promoted Nrf2 expression as well as its downstream targets including HO-1, NQO-1, GCLC, and GCLM. Collectively, these findings suggest that HMGB1 regulates glucose-induced ferroptosis via Nrf2 pathway in mesangial cells.
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http://dx.doi.org/10.1042/BSR20202924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897919PMC
February 2021

[Comparative study between electroacupuncture at Neima point and Neiguan (PC 6) and epidural nerve block for preemptive analgesia in patients undergoing thoracic surgery].

Zhongguo Zhen Jiu 2021 Jan;41(1):59-64

Clinical Medical School/First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.

Objective: To compare the clinical effect between electroacupuncture (EA) at Neima point and Neiguan (PC 6) and epidural nerve block for preemptive analgesia in patients undergoing thoracic surgery.

Methods: Sixty patients with elective radical esophagectomy were randomly divided into a group A, a group B and a control group, 20 cases in each group. The patients in the group A were treated with injection of 20 mL 0.375% ropivacaine at epidural space 30 min before anesthesia induction, followed by normal anesthesia during operation; the patients in the group B were treated with 30 min EA at bilateral Neima point and Neiguan (PC 6) before anesthesia induction, followed by normal anesthesia during operation; the patients in the control group were treated with general anesthesia alone. Patient-controlled intravenous analgesia was applied for all the patients. The mean arterial pressure (MAP) and heart rate (HR) were recorded at the following time points: before acupuncture/epidural puncture (T), skin incision (T), extubation (T) and 2 h after operation (T); the dosage of anesthetics and extubation time were recorded; the plasma levels of β-endorphin (β-EP), 5-hydroxytryptamine (5-HT) and prostaglandin E (PGE) were measured at the following time points: T, T, 12 h after operation (T), 24 h after operation (T) and 48 h after operation (T). Visual analogue scale (VAS) was used to evaluate the analgesic effect.

Results: The MAP at T and T in the group A was lower than that in group B and control group (<0.05), and HR at T and T was lower than that in control group (<0.05). The MAP and HR at T and T in the group B were lower than those in the control group (<0.05). The dosage of remifentanil in the group A and group B was lower than that in the control group (<0.05), and extubation time was earlier than that in the control group (<0.05). The content of β-EP at T, T and T in the group B was higher than that in the group A and control group (<0.05); the contents of 5-HT and PGE at T, T and T in the group A and group B were lower than those in the control group (<0.05). The VAS scores at T, T and T in the group A and group were lower than those in the control group (<0.05).

Conclusion: The preemptive analgesia of EA at Neima point and Neiguan (PC 6) and epidural nerve block could both provide effective perioperative analgesia for thoracic surgery. The EA could better maintain intraoperative hemodynamics and has less physiological disturbance.
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http://dx.doi.org/10.13703/j.0255-2930.20200121-k0001DOI Listing
January 2021

Magnesium dietary intake and physical activity in Type 2 diabetes by gender in White, African-American and Mexican American: NHANES 2011-2014.

Endocrinol Diabetes Metab 2021 01 20;4(1):e00203. Epub 2020 Nov 20.

Department of Bioinformatics and Biostatistics School of Public Health and Information Sciences University of Louisville Louisville KY USA.

Aims: To analyse the causal relationships of nutrition intake and physical activity on haemoglobin A1c (HbA1C) in patients diagnosed with type 2 diabetes mellitus (T2DM) stratified by gender and ethnicity.

Materials And Methods: An historical cohort of patients with diagnosed T2DM (n = 2831) was extracted from the National Health and Nutrition Examination Survey (NHANES) 2011-2014 public database, including but not limited to, measurements of physical activity, nutrition, body mass index (BMI) and HbA1c. Multivariate analyses and path analyses were employed to estimate the regression coefficients and path coefficients () of causal path models of physical activity and nutrition intake on HbA1c stratified by gender and three ethnicity groups (ie non-Hispanic white, non-Hispanic black and Mexican American).

Results: A significant causal path from increased physical activity to increased magnesium (Mg) intake to decreased HbA1c was found. In addition, increased physical activity significantly decreased BMI, which further decreased HbA1c. These results varied by gender and ethnicity but were directionally consistent. Physical activity decreased HbA1c through BMI for males and through Mg intake for females. Mexican American decreased HbA1c through Mg intake, while non-Hispanic black had an increased HbA1c due to its ethnicity and through increased BMI.

Conclusions: The beneficial effects of physical activity on decreased HbA1c were mediated through the increased Mg intake and decreased BMI. This aligned with recent investigations of the inverse causal association of Mg intake with insulin resistance and with decreased inflammation.
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http://dx.doi.org/10.1002/edm2.203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831210PMC
January 2021

Investor attention and cryptocurrency: Evidence from the Bitcoin market.

PLoS One 2021 1;16(2):e0246331. Epub 2021 Feb 1.

College of Economics, Shenzhen University, Shenzhen, Guangdong, China.

This paper adds to the growing literature of cryptocurrency and behavioral finance. Specifically, we investigate the relationships between the novel investor attention and financial characteristics of Bitcoin, i.e., return and realized volatility, which are the two most important characteristics of one certain asset. Our empirical results show supports in the behavior finance area and argue that investor attention is the granger cause to changes in Bitcoin market both in return and realized volatility. Moreover, we make in-depth investigations by exploring the linear and non-linear connections of investor attention on Bitcoin. The results indeed demonstrate that investor attention shows sophisticated impacts on return and realized volatility of Bitcoin. Furthermore, we conduct one basic and several long horizons out-of-sample forecasts to explore the predictive ability of investor attention. The results show that compared with the traditional historical average benchmark model in forecasting technologies, investor attention improves prediction accuracy in Bitcoin return. Finally, we build economic portfolios based on investor attention and argue that investor attention can further generate significant economic values. To sum up, investor attention is a non-negligible pricing factor for Bitcoin asset.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246331PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850507PMC
February 2021

Adaptive Neural Network Control for Full-State Constrained Robotic Manipulator With Actuator Saturation and Time-Varying Delays.

IEEE Trans Neural Netw Learn Syst 2021 Jan 27;PP. Epub 2021 Jan 27.

This article proposes an adaptive neural network (NN) control method for an n-link constrained robotic manipulator. Driven by actual demands, manipulator and actuator dynamics, state and input constraints, and unknown time-varying delays are taken into account simultaneously. NNs are employed to approximate unknown nonlinearities. Time-varying barrier Lyapunov functions are utilized to cope with full-state constraints. By resorting to saturation function and Lyapunov-Krasovskii functionals, the effects of actuator saturation and time delays are eliminated. It is proved that all the closed-loop signals are semiglobally uniformly ultimately bounded, full-state constraints and actuator saturation are not violated, and error signals remain within compact sets around zero. Simulation studies are given to demonstrate the validity and advantages of this control scheme.
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http://dx.doi.org/10.1109/TNNLS.2021.3051946DOI Listing
January 2021

Characterization and cytocompatibility of 3D porous biomimetic scaffold derived from rabbit nucleus pulposus tissue in vitro.

J Mater Sci Mater Med 2021 Jan 20;32(1). Epub 2021 Jan 20.

Department of Orthopaedics, General Hospital of Southern Theatre Command of PLA, Guangzhou, 510010, Guangdong, PR China.

Intervertebral disc (IVD) degeneration is one of the most important causes of lower back pain. Tissue engineering provides a new method for the experimental treatment of degenerative disc diseases. This study aims to develop a natural, acellular, 3D interconnected porous scaffold derived from the extracellular matrix (ECM) of nucleus pulposus. The nucleus pulposus (NP) was decellularized by sequential detergent-nuclease methods, including physical crushing, freeze-drying and cross-linking. These 3D porous scaffolds were fabricated with a high porosity of (81.28 ± 4.10)%, an ideal pore size with appropriate mechanical properties. Rabbit bone marrow mesenchymal stem cells (rBMSCs) were seeded and cultured on the scaffolds. And the mechanical tests showed the compressive elastic modulus of the scaffolds cultured for 4 weeks reached 0.12 MPa, which was better than that of the scaffolds cultured for 2 weeks (0.07 MPa) and that of the control group (0.04 MPa). Scanning electron microscopy (SEM), histological assays, molecular biology assays revealed that the scaffolds could provide an appropriate microstructure and environment for the adhesion, proliferation, migration and secretion of seeded cells in vitro. As assays like histology, immunohistochemistry and the real-time qRT-PCR showed, NP-like tissues were preliminarily formed. In conclusion, the 3D porous scaffold derived from NP ECM is a potential biomaterial for the regeneration of NP tissues. A natural, acellular, 3D interconnected porous scaffold derived from the extracellular matrix (ECM) of nucleus pulposus was developed by sequential detergent-nuclease and freeze-drying method, which can reduce the damage of protein activity to the minimum. It is very similar to the composition and internal environment of the natural nucleus pulposus, because it derived from the natural nucleus pulposus. Scanning electron microscopy (SEM), histological assays, molecular biology assays revealed that the scaffolds could provide an appropriate microstructure and environment for the adhesion, proliferation, migration, and secretion of seeded cells in vitro.
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http://dx.doi.org/10.1007/s10856-020-06480-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817588PMC
January 2021

Minimizing salinity accumulation via regulating draw solute concentration in a bioelectrochemically assisted osmotic membrane bioreactor.

Chemosphere 2021 Jun 11;272:129613. Epub 2021 Jan 11.

School of Environment, Nanjing Normal University, Wenyuan Road 1, Nanjing, 210023, China. Electronic address:

A suitable draw solute (DS) concentration in bioelectrochemically assisted osmotic membrane bioreactor (BEA-OMBR) can convert the "negative effect" of salinity accumulation into a "beneficial effect" by using the reverse-fluxed DS as a buffer agent or a carbon source supplement. Herein, the effect of DS concentration from acid buffer solution (i.e., ammonium chloride, NHCl), alkaline buffer solution (i.e., sodium bicarbonate, NaHCO), and organic solution (i.e., sodium acetate, NaOAc) on salinity accumulation was systematically investigated. Salinity accumulation with NaHCO DS mainly derived from reversal fluxed sodium ion (Na, major contributor with DS concentration ≤0.25 M) and bicarbonate ion (main contributor with DS concentration ≥0.50 M): Na accumulation could be mitigated by Na transport dominant by electrically driven migration (i.e., 21.3-62.1% of reverse-fluxed Na), and bicarbonate accumulation could be reduced by buffer system. A medium-low concentration of 0.25 M NHCl DS had a better performance on current density of 165.0 ± 23.0 A m and COD removal efficiency of 91.5 ± 3.4% by taking advantage that 77.7 ± 1.3% of reverse-fluxed ammonium could be removed by biological treatment and ammonium transport. A high NaOAc DS concentration (i.e., ≥0.05 M) exhibited a higher current density of 145.3-146.0 A m but a lower COD removal efficiency due to the limited carbon source utilization capacity of anaerobic bacteria. Both concentration diffusion (20.9-28.3%) and electrically driven migration (29.5-39.4%) promoted reverse-fluxed Na transport to catholyte and thus mitigated Na accumulation in the feed/anolyte. These findings have provided an optimal DS concentration for BEA-OMBR operation and thus encourage its further development.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129613DOI Listing
June 2021

Effect of Shenkang on renal fibrosis and activation of renal interstitial fibroblasts through the JAK2/STAT3 pathway.

BMC Complement Med Ther 2021 Jan 6;21(1):12. Epub 2021 Jan 6.

Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing, 100029, China.

Background: Activation of renal fibroblasts is a critical mechanism in the process of renal fibrosis. As a commonly used herbal formula, Shenkang (SK) has been found to attenuate renal fibrosis and renal parenchyma destruction. However, the effect of SK on renal fibroblast activation in unilateral ureteral obstruction (UUO) mice and its molecular mechanism remain undetermined. The present study was performed to elucidate the effect of SK on renal fibroblast activation and renal fibrosis, as well as the potential underlying mechanism, in both NRK-49F cells and UUO mice.

Methods: NRK-49F cells were stimulated with 10 ng/ml TGF-β1 for 48 h. After SK treatment, the CCK-8 method was used to evaluate cell viability. Thirty-six C57BL/6 mice were randomly divided into the sham group, UUO group, angiotensin receptor blocker (ARB) group, and SK high-, moderate- and low-dose groups. UUO was induced in mice except those in the sham group. Drugs were administered 1 day later. On the 13th day, the fractional anisotropy (FA) value was determined by MRI to evaluate the degree of renal fibrosis. After 14 days, serum indexes were assessed. Hematoxylin and eosin (HE) and Sirius red staining were used to observe pathological morphology and the degree of fibrosis of the affected kidney. Western blotting and PCR were used to assess the expression of related molecules in both cells and animals at the protein and gene levels.

Results: Our results showed that SK reduced extracellular matrix (ECM) and α-smooth muscle actin (α-SMA) expression both in vitro and in vivo and attenuated renal fibrosis and the pathological lesion degree after UUO, suppressing JAK2/STAT3 activation. Furthermore, we found that SK regulated the JAK2/STAT3 pathway regulators peroxiredoxin 5 (Prdx5) in vitro and suppressor of cytokine signaling protein 1 (SOCS1) and SOCS3 in vivo.

Conclusions: These results indicated that SK inhibited fibroblast activation by regulating the JAK2/STAT3 pathway, which may be a mechanism underlying its protective action in renal fibrosis.
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http://dx.doi.org/10.1186/s12906-020-03180-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789243PMC
January 2021

Association of Multiple Dopamine D3 Receptor Gene 3'UTR Polymorphisms with Susceptibility to Parkinson's Disease and Clinical Efficacy of Piribedil Therapy.

Genet Test Mol Biomarkers 2021 Jan 28;25(1):20-30. Epub 2020 Dec 28.

Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.

To investigate the correlation between the Dopamine D3 receptor () 3'untranslated region (3'UTR) gene polymorphism and susceptibility to Parkinson's disease (PD) and the clinical effect of the DRD2 and DRD3 agonist piribedil treatment. Sanger sequencing was used to analyze the single nucleotide polymorphisms (SNPs) within the 3'UTR rs76126170, rs9868039, rs9817063, and rs3732790 loci of the gene in 284 PD patients and 284 controls. PD patients were treated with piribedil sustained-release tablets (50 mg) combined with levodopa and benserazide hydrochloride tablets three times daily (patients with first-diagnosed PD were only administrated with piribedil sustained-release tablets) for 3 months. The Unified Parkinson's Disease Rating Scale (UPDRS) and the Hoehn and Yahr disease stage were evaluated at baseline and after 3 months of treatment. The T allele carriers of the gene rs76126170 locus were more susceptible to PD than the C allele carriers (odds ratio [OR] = 3.44, 95% confidence interval [CI]: 2.46-4.80,  < 0.01). Carriers of the rs9868039 A allele had a decreased risk of PD compared to those with G allele (OR = 0.67, 95% CI: 0.53-0.86,  < 0.01). C allele carriers at rs9817063 were less likely to develop PD than those with T allele (OR = 0.74, 95% CI: 0.58-0.94,  = 0.02). No significant correlation was observed between the alleles or genotypes of the rs3732790 locus and PD susceptibility ( > 0.05). The various genotypes of the gene loci rs76126170, rs9868039, and rs9817063 in PD patients were associated with significant differences with regard to reduction of UPDRS scores and Hoehn and Yahr stage after 3 months of treatment ( < 0.05). The alleles and genotypes of the gene 3' UTR SNP loci rs76126170, rs9868039, and rs9817063 are associated with PD susceptibility and the clinical efficacy of piribedil treatment.
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http://dx.doi.org/10.1089/gtmb.2020.0195DOI Listing
January 2021