Publications by authors named "You Wang"

356 Publications

OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.

Mol Med 2021 Apr 9;27(1):37. Epub 2021 Apr 9.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Objective: Ovarian tumour domain deubiquitinase with linear linkage specificity (OTULIN) is a potent negative regulator of the nuclear factor-κB (NF-κB) signalling pathway, and it plays a strong neuroprotective role following acute ischemic stroke. Electroacupuncture (EA) is an effective adjuvant treatment for reducing brain injury and neuroinflammation via the inhibition of NF-κB p65 nuclear translocation, but the underlying mechanism is not clear. The present study investigated whether OTULIN was necessary for EA to mitigate brain injury and glial cell activation in a transient middle cerebral artery occlusion (tMCAO) model in rats.

Methods: An acute ischaemic stroke model was established via tMCAO surgery in Sprague-Dawley (SD) rats. EA was performed once daily at "Baihui (GV 20)", "Hegu (LI 4)", and "Taichong (LR 3)" acupoints. The effect of EA on the spatiotemporal expression of OTULIN in the ischaemic penumbra of the cerebral cortex was detected within 7 days after reperfusion. The effects of OTULIN gene silencing on EA neurological deficits, cerebral infarct volume, neuronal damage, the activation of microglia and astrocytes, the contents of tumour necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), and the expression of p-IκBa, IκBa and nucleus/cytoplasm NF-κB p65 protein were assessed.

Results: EA treatment increased endogenous OTULIN expression, which peaked at 48 h. Enhanced OTULIN was primarily located in neurons, but a small amount of OTULIN was detected in microglia. OTULIN silencing obviously reversed EA neuroprotection, which was demonstrated by worsened neurobehavioural performance, cerebral infarct volume and neuronal injury. The inhibitory effect of EA on the NF-κB pathway was also attenuated by enhanced IκBα phosphorylation and NF-κB p65 nuclear translocation. EA partially inhibited the transformation of microglia and astrocytes from resting states to activated states and reduced the secretion of TNF-α, IL-1β and IL-6. However, these preventive effects were reversed after the silencing of OTULIN expression.

Conclusions: OTULIN provides a new potential therapeutic target for EA to alleviate acute ischaemic stroke-induced brain injury and the activation of glial cells, which are related to suppression of the NF-κB signalling pathway.
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http://dx.doi.org/10.1186/s10020-021-00297-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035756PMC
April 2021

The enrichment of neutrophil extracellular traps impair the placentas of systemic lupus erythematosus through accumulating decidual NK cells.

Sci Rep 2021 Mar 25;11(1):6870. Epub 2021 Mar 25.

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.

Despite the advances made in the management of pregnancies in women with systemic lupus erythematosus (SLE), the rate of adverse pregnancy outcomes is still higher than that in the general population. In the last few years, neutrophil extracellular traps (NETs) were proven to be detrimental in both autoimmune diseases and placental injury. We investigated whether NETs could be detected in the placentas of pregnant individuals with SLE and explored the relationship between NETs and decidual natural killer cells (dNKs), which comprise the majority of immune cells at the maternal-fetal interface, using clinical samples and animal models. In this study, we found that the infiltration of NETs and dNKs, especially CD56CD16 NK cells, was significantly increased in pregnant individuals with SLE with placental insufficiency. In the murine models of SLE, the number of dNKs was significantly decreased due to the decreased formation of NETs affected by Ly6G. Moreover, the histopathological placental injury was reduced, with a remarkable increase in fetal birth weight. This study shows that NETs may contribute to immunological disorder in the placenta and the pathological changes in pregnancies with SLE, which provides a research basis for further explorations of the mechanism of SLE in placental impairment.
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http://dx.doi.org/10.1038/s41598-021-86390-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994714PMC
March 2021

DNA methylation occurring in Cre-expressing cells inhibits loxP recombination and silences loxP-sandwiched genes.

New Phytol 2021 Mar 20. Epub 2021 Mar 20.

Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops; Biotechnology Research Center, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing, 400715, P. R. China.

● The low DNA recombination efficiency of site-specific recombinase systems in plants limits their application; however, the underlying mechanism is unknown. ● We evaluate the gene deletion performance of four recombinase systems (Cre/loxP, Flp/FRT, KD/KDRT, and B3/B3RT) in tobacco where the recombinases are under the control of germline-specific promoters. ● We find that the expression of these recombinases results mostly in gene silencing rather than gene deletion. Using the Cre/loxP system as a model, we reveal that the region flanked by loxP sites (floxed) is hypermethylated, which prevents floxed genes from deletion while silences the expression of the genes. We further show CG methylation alone in recombinase binding element of loxP site is unable to impede gene deletion; instead, CHH methylation in the crossover region is required to inhibit loxP recombination. ● Our study illustrates the important role of recombinase-induced DNA methylation in the inhibition of site-specific DNA recombination and uncovers the mechanism underlying the recombinase-associated gene silence in plants.
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http://dx.doi.org/10.1111/nph.17353DOI Listing
March 2021

p300/CBP inhibitor A-485 inhibits the differentiation of osteoclasts and protects against osteoporotic bone loss.

Int Immunopharmacol 2021 Feb 21;94:107458. Epub 2021 Feb 21.

Department of Bone and Joint Surgery, Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, China. Electronic address:

Osteoporosis is one of the most common metabolic bone diseases among pre- and post-menopausal women. Despite numerous advances in the treatment of osteoporosis in recent years, the outcomes remain poor due to severe side effects. In this study, we investigated whether A-485, a highly selective catalytic p300/CBP inhibitor, could attenuate RANKL-induced osteoclast differentiation and explored the underlying molecular mechanisms. The protective role of A-485 in osteoporosis was verified using a mouse model of ovariectomy (OVX)-induced bone loss and micro-CT scanning. A-485 inhibited RANKL-induced osteoclast differentiation in vitro by reducing the number of tartrate-resistant acid phosphatase-positive osteoclasts without inducing significant cytotoxicity. In particular, A-485 dose-dependently disrupted F-actin ring formation and downregulated the expression of genes associated with osteoclast differentiation, such as CTSK, c-Fos, TRAF6, VATPs-d2, DC-STAMP, and NFATc1, in a time- and dose-dependent manner. Moreover, A-485 inhibited the RANKL-induced phosphorylation of MAPK pathways and attenuated OVX-induced bone loss in the mouse model while rescuing the loss of bone mineral density. Our in vitro and in vivo findings suggest for the first time that A-485 has the potential to prevent postmenopausal osteoporosis and could therefore be considered as a therapeutic molecule against osteoporosis.
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http://dx.doi.org/10.1016/j.intimp.2021.107458DOI Listing
February 2021

Risk of Spinal Hematoma After Lumbar Puncture.

JAMA 2021 02;325(8):787

Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

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http://dx.doi.org/10.1001/jama.2020.24595DOI Listing
February 2021

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study.

Biomed Environ Sci 2021 Jan;34(1):9-18

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Objective: The relationship between serum uric acid (SUA) levels and glycemic indices, including plasma glucose (FPG), 2-hour postload glucose (2h-PG), and glycated hemoglobin (HbA1c), remains inconclusive. We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.

Methods: The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study. A total of 105,922 community-dwelling adults aged ≥ 40 years underwent the oral glucose tolerance test and uric acid assessment. The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.

Results: A total of 30,941 men and 62,361 women were eligible for the current analysis. Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels, but with different inflection points in men and women. The thresholds for FPG, 2h-PG, and HbA1c for men and women were 6.5/8.0 mmol/L, 11.0/14.0 mmol/L, and 6.1/6.5, respectively (SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).

Conclusion: An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes, while the inflection points were reached earlier in men than in women.
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http://dx.doi.org/10.3967/bes2021.003DOI Listing
January 2021

Advances in precise regulation of CRISPR/Cas9 gene editing technology.

Yi Chuan 2020 Dec;42(12):1168-1177

Biotherapy Center, the Seventh Medical Center of PLA General Hospital, Beijing 100700, China.

Gene editing is a genetic engineering technology that can modify, delete, or insert a small piece of DNA at a specific point in the genome of cells and organisms. Gene editing technology holds great promises in the fields of disease treatment, gene function regulation, gene detection, drug research and development, and crop breeding. However, side effects, such as off-target editing, genotoxicity and other issues, have gradually emerged in the application. In the CRISPR (clustered regularly interspaced short palindromic repeats) system, the Cas9 nuclease can specifically recognize the target DNA by the base pairing of a guide RNA (gRNA) with the target DNA. Upon target recognition, the two DNA strands are cleaved by distinct domains of the Cas9 nuclease. Since both Cas9 nuclease and gRNA possess different characteristics in their own activities, recognition sites and binding ability to specific target, it is essential to precisely regulate the activity of Cas9 nuclease and gRNA in both time and space manners, thus preventing the risk of side effects and enhancing the precise regulation of the CRISPR/Cas9 gene editing technology. In this review, we summarize the advances in the precise control of gene editing, especially CRISPR/cas9 over several dimensions using fusion Cas9 proteins regulated by light, temperature and drugs, exploiting and screening anti-CRISPRs proteins, synthesizing and identifying small molecules- inhibitors, and developing other therapeutic agents, thereby providing a reference and research ideas for human disease treatment, crop and livestock improvement and prevention of biotechnology misuse.
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http://dx.doi.org/10.16288/j.yczz.20-069DOI Listing
December 2020

Photothermal Generation of Oxygen-Irrelevant Free Radicals with Simultaneous Suppression of Glutathione Synthesis for an Enhanced Photonic Thermodynamic Cancer Therapy.

ACS Biomater Sci Eng 2020 11 2;6(11):6186-6194. Epub 2020 Oct 2.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Reactive oxygen species (ROS) as one kind of oxygen-dependent toxic free radical has been widely adopted in ROS-mediated therapies (RMT). However, two shackles, known as tumor hypoxia and high intratumor glutathione (GSH) levels, respectively, limit RMT as the former prevents ROS from generating, while the latter scavenges the ROS generated. Herein, we developed a novel nanoplatform to simultaneously overcome the two shackles by facile preparation of mesoporous carbon nanospheres (MCNs) with AIBI and raloxifene loaded inside as an initiator and inhibitor, respectively, and phase-change materials (PCMs) capped outside as gatekeepers. Upon 808 nm NIR light irradiation, the photothermal effect of MCNs melted PCMs causing the loaded AIBI and raloxifene to be released, during which the AIBI was further decomposed into oxygen-irrelevant radicals to kill cancer cells, while the raloxifene suppressed GSH synthesis, synergistically enabling an enhanced photonic thermodynamic cancer therapy.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00889DOI Listing
November 2020

Image reconstruction through a multimode fiber with a simple neural network architecture.

Sci Rep 2021 Jan 13;11(1):896. Epub 2021 Jan 13.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.

Multimode fibers (MMFs) have the potential to carry complex images for endoscopy and related applications, but decoding the complex speckle patterns produced by mode-mixing and modal dispersion in MMFs is a serious challenge. Several groups have recently shown that convolutional neural networks (CNNs) can be trained to perform high-fidelity MMF image reconstruction. We find that a considerably simpler neural network architecture, the single hidden layer dense neural network, performs at least as well as previously-used CNNs in terms of image reconstruction fidelity, and is superior in terms of training time and computing resources required. The trained networks can accurately reconstruct MMF images collected over a week after the cessation of the training set, with the dense network performing as well as the CNN over the entire period.
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http://dx.doi.org/10.1038/s41598-020-79646-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806887PMC
January 2021

A partial hemi-resurfacing preliminary study of a novel magnetic resonance imaging compatible polyetheretherketone mini-prosthesis for focal osteochondral defects.

J Orthop Translat 2021 Jan 20;26:67-73. Epub 2020 Mar 20.

Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Background: The use of partial articular resurfacing surgery with a mini-implant has been gradually increasing; the implant is mainly made of cobalt-chromium metal material, and cartilage changes cannot be monitored after implantation. Thus, we aimed to develop a novel local articular resurfacing polyetheretherketone (PEEK) mini-implant and investigate its feasibility for postoperative magnetic resonance imaging (MRI) monitoring of implant location, bone changes, and cartilage degeneration without artefacts.

Methods: Nine skeletally mature female standardised goats were used and divided into the sham, PEEK, and cobalt-chromium-molybdenum alloy (Co-Cr-Mo) groups. The animals underwent local articular resurfacing operation with Co-Cr-Mo alloy (Co-Cr-Mo group) and PEEK (PEEK group) mini-implants. X-ray, computed tomography, and MRI examinations were performed at 12 weeks postoperatively. The sham group underwent a similar surgical procedure to expose the femoral head but without implantation. Gross necropsy and surface topography measurement of the articular cartilage of the acetabulum were performed after sacrificing the animals. Imaging artefacts and opposing cartilage degeneration in the acetabulum were also examined.

Results: Cartilage damage occurred in both the Co-Cr-Mo and PEEK groups, and the damaged cartilage area was markedly larger in the Co-Cr-Mo group than in the PEEK group, as assessed by gross necropsy and histological staining. The mean surface roughness of the opposing cartilage was approximately 65.3, 117.4, and 188.4 ​μm ​at 12 weeks in the sham, PEEK, and Co-Cr-Mo groups, respectively. The Co-Cr-Mo mini-implant was visualised on radiographs, but computed tomography and MR images were markedly affected by artefacts, whereas the opposing cartilage and surrounding tissue were clear on MR images in the PEEK group. Opposing cartilage damage and subchondral bone marrow oedema could be detected by MRI in the PEEK group.

Conclusions: The PEEK mini-implant can be a novel alternative to the Co-Cr-Mo mini-implant in articular resurfacing to treat focal osteochondral defects with less cartilage damage. It is feasible to postoperatively monitor the PEEK implant location, surrounding bone changes, and opposing cartilage degeneration by MRI without artefacts.

The Translational Potential Of This Article: The use of MRI to monitor changes in the opposing cartilage after prosthesis implantation has not been widely applied because MR images are generally affected by artefacts generated by the metal prosthesis. This study revealed that the PEEK mini-implant can be a novel alternative to the Co-Cr-Mo mini-implant in articular resurfacing to treat focal osteochondral defects, and it is feasible to monitor the PEEK implant location, surrounding bone changes, and opposing cartilage damage/degeneration by MRI without artefacts postoperatively.
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http://dx.doi.org/10.1016/j.jot.2020.02.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773958PMC
January 2021

Group 11 Transition-Metal Halide Monolayers: High Promises for Photocatalysis and Quantum Cutting.

J Phys Chem Lett 2021 Jan 30;12(1):525-531. Epub 2020 Dec 30.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China.

Recently, two-dimensional (2D) metal halides have triggered an enormous interest for their tunable mechanical, electronic, magnetic, and topological properties, greatly enriching the family of 2D materials. Here, based on first-principles calculations, we report a systematic study of group 11 transition-metal halide MX (M = Cu, Ag, Au; X = Cl, Br, I) monolayers. Among them, CuBr, CuI, AgBr, and AgI monolayers exhibit high thermodynamic, dynamic, and mechanic stability. The four stable monolayers have a direct band gap of ∼3.12-3.36 eV and possess high carrier mobility (∼10 cm V s), suggestive of future photocatalysts for water splitting applications. What is more, the simulations of optical properties confirm that the stable MX monolayers hold the potential for further applications in ultraviolet optical devices and quantum cutting solar materials.
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http://dx.doi.org/10.1021/acs.jpclett.0c03138DOI Listing
January 2021

Engineering SnO nanorods/ethylenediamine-modified graphene heterojunctions with selective adsorption and electronic structure modulation for ultrasensitive room-temperature NO detection.

Nanotechnology 2021 Apr;32(15):155505

School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, People's Republic of China.

Ever-increasing concerns over air quality and the newly emerged internet of things (IoT) for future environmental monitoring are stimulating the development of ultrasensitive room-temperature gas sensors, especially for nitrogen dioxide (NO), one of the most harmful air pollution species released round-the-clock from power plants and vehicle exhausts. Herein, tin dioxide nanorods/ethylenediamine-modified reduced graphene oxide (SnO/EDA-rGO) heterojunctions with selective adsorption and electronic structure modulation were engineered for highly sensitive and selective detection of NO at room temperature. The modified EDA groups not only enable selective adsorption to significantly enrich NO molecules around the interface but also realize a favorable modulation of SnO/EDA-rGO electronic structure by increasing the Fermi level of rGO, through which the sensing performance of NO is synergistically enhanced. The response of the SnO/EDA-rGO sensor toward 1 ppm NO reaches 282%, which exceeds the corresponding SnO/rGO sensor by a factor of 2.8. It also exhibits a low detection limit down to 100 ppb, enhanced selectivity, and rapid response/recovery kinetics. This approach to designing a novel heterojunction with significantly enhanced chemical and electric effects may shed light on the future engineering of gas-sensing materials.
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http://dx.doi.org/10.1088/1361-6528/abd657DOI Listing
April 2021

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Cell Metab 2021 Apr 14;33(4):804-817.e5. Epub 2020 Dec 14.

Sarah W. Stedman Nutrition and Metabolism Center and Duke Molecular Physiology Institute, Duke University Medical Center, Durham, NC 27701, USA; Department of Medicine, Endocrinology and Metabolism Division, Duke University Medical Center, Durham, NC 27701, USA; Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27701, USA. Electronic address:

Metabolic fuels regulate insulin secretion by generating second messengers that drive insulin granule exocytosis, but the biochemical pathways involved are incompletely understood. Here we demonstrate that stimulation of rat insulinoma cells or primary rat islets with glucose or glutamine + 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (Gln + BCH) induces reductive, "counter-clockwise" tricarboxylic acid (TCA) cycle flux of glutamine to citrate. Molecular or pharmacologic suppression of isocitrate dehydrogenase-2 (IDH2), which catalyzes reductive carboxylation of 2-ketoglutarate to isocitrate, results in impairment of glucose- and Gln + BCH-stimulated reductive TCA cycle flux, lowering of NADPH levels, and inhibition of insulin secretion. Pharmacologic suppression of IDH2 also inhibits insulin secretion in living mice. Reductive TCA cycle flux has been proposed as a mechanism for generation of biomass in cancer cells. Here we demonstrate that reductive TCA cycle flux also produces stimulus-secretion coupling factors that regulate insulin secretion, including in non-dividing cells.
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http://dx.doi.org/10.1016/j.cmet.2020.11.020DOI Listing
April 2021

Designing high-performance hypergolic propellants based on materials genome.

Sci Adv 2020 Dec 4;6(49). Epub 2020 Dec 4.

Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA.

A new generation of rocket propellants for deep space exploration, ionic liquid propellants, with long endurance and high stability, is attracting more and more attention. However, a major defect of ionic liquid propellants that restricts their application is the inadequate hypergolic reactivity between the fuel and the oxidant, and this defect results in local burnout and accidental explosions during the launch process. We propose a visualization model to show the features of structure, density, thermal stability, and hypergolic activity for estimating propellant performances and their application abilities. This propellant materials genome and visualization model greatly improves the efficiency and quality of developing high-performance propellants, which benefits the discovery of new advanced functional molecules in the field of energetic materials.
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http://dx.doi.org/10.1126/sciadv.abb1899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717915PMC
December 2020

Traditional Chinese Medicines alleviated myocardial ischemia by regulating Qi and promoting blood circulation.

J Tradit Chin Med 2020 12;40(6):974-982

Out-patient Department, Chinese PLA General Hospital, Beijing 100853, China.

Objective: To investigate the efficacy of Chinese medicines on Qi stagnation and blood stasis in rats with myocardial ischemia.

Methods: Fifty male Wistar rats were randomly divided into five groups (n = 10) as follows: (a) sham operation (Sham), (b) myocardial ischemia (Model), (c) treatment that regulates Qi (Qi), (d) treatment that promotes blood circulation (Blood), (e) treatment that both regulates Qi and promotes blood circulation (QB). The rat model was established via activities restriction for 6 h followed by tail clamp stimulation for 5 mins every day for 7 d and occlusion left coronary anterior descending artery. Afterwards rats were treated with medicines that regulate Qi and/or promote blood circulation via gavage for 14 d. Behavioral parameters were evaluated using open field and elevated plus-maze tests. The tongue color and sublingual vein were visually examined. Blood flow perfusion of tongue and auricle were detected using PIM Ⅱ. The mesenteric microcirculation was examined via capillaroscopy, and hemodynamics was assessed using a polygraph system. Serum homocysteine (Hcy), creatine kinase isoenzyme (CKMB) levels and endothelin-1 (ET-1) were measured. Hematoxylin and eosin staining and transmission electron microscopy were employed to detect the myocardial morphology and ultrastructure, respectively.

Results: Compared with findings in Sham group, rats in model group had coarse hair, dark mucosa of the lips and claw, low activity, and increased anxiety. Compared with findings in Model group, rats in the three treatment groups exhibited a lighter tongue color without an extended and varicose sublingual vein. There were significant increases of auricle blood flow perfusion in the Qi group and tongue bottom blood flow perfusion in the QB group. Compared with findings in Model rats, rats in Blood group exhibited improved mesenteric microcirculation associated with increased mesenteric blood flow and a larger arteriole diameter. Moreover, compared with findings in Model rats, Qi and QB rats exhibited increased left ventricular ± dp/dtmax, decreased serum CKMB, Hcy, ET-1 levels, and reduced myocardial ultrastructural damage.

Conclusion: Myocardial ischemia damage was suppressed by Traditional Chinese Medicines that regulate Qi and promote blood circulation.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2020.06.009DOI Listing
December 2020

Overcoming Planktonic and Intracellular -Associated Infection with a Cell-Penetrating Peptide-Conjugated Antimicrobial Peptide.

ACS Infect Dis 2020 12 25;6(12):3147-3162. Epub 2020 Nov 25.

Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China.

is a primary pathogen responsible for causing postoperative infections as it survives and persists in host cells, including osteoblasts and macrophages. These cells then serve as reservoirs resulting in chronic infections. Most traditional antibiotics have poor effects on intracellular because they cannot enter the cell. Herein, a cell-penetrating peptide TAT-KR-12 was derived from the trans-activating transcription (TAT) peptide and KR-12 (residues 18-29 of human cathelicidin LL-37). The TAT acts as a "trojan horse" to deliver KR-12 peptide into the cells to kill . Moreover, effective antibacterial properties and biocompatibility were observed , demonstrating that TAT-KR-12 is effective not only in eliminating planktonic , but also in eliminating intracellular cells . TAT-KR-12, as with LL-37, also elicits strong anti-inflammatory activities in LPS-stimulated macrophages, as demonstrated by significant inhibition of NO, TNF-α, and IL-1β expression and secretion from LPS-stimulated RAW264.7 cells. In the subcutaneous infection mouse model of planktonic and intracellular infections, the growth of is evidently inhibited without cytotoxicity. These results suggest that the novel antimicrobial TAT-KR-12 may prove to be an effective treatment option to overcome antibiotic resistance caused by intracellular bacterial infections.
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http://dx.doi.org/10.1021/acsinfecdis.0c00264DOI Listing
December 2020

Letter by Xu et al Regarding Article, "Cilostazol for Secondary Prevention of Stroke and Cognitive Decline: Systematic Review and Meta-Analysis".

Stroke 2020 12 23;51(12):e375-e376. Epub 2020 Nov 23.

Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, China.

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http://dx.doi.org/10.1161/STROKEAHA.120.031872DOI Listing
December 2020

Human Amniotic Epithelial Cells Alleviate a Mouse Model of Parkinson's Disease Mainly by Neuroprotective, Anti-Oxidative and Anti-Inflammatory Factors.

J Neuroimmune Pharmacol 2020 Nov 9. Epub 2020 Nov 9.

State Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, 200127, Shanghai, China.

Human amniotic epithelial cells (hAECs) have been reported to have neuroprotective roles in Parkinson's disease (PD) animal models. However, the molecular mechanism is not fully understood. The present study was designed to explore the possible mechanism by which hAECs ameliorate PD symptoms and the important paracrine factors produced by hAECs that attribute to the recovery of dopaminergic neurons. Thus, we performed in vivo and in vitro experiments with hAECs in PD models or lesioned dopaminergic neurons, respectively. First, hAECs were transplanted into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice and motor deficits were significantly attenuated. Second, the grafts prevented the loss of nigral dopaminergic neurons and promoted the outgrowth of neurites and striatal axon fibers in PD mice. In addition, decreased microglial activation, inflammatory factor levels and MPTP-induced excessive reactive oxygen species (ROS) levels were also observed in hAEC-treated PD mice. In vitro, we found that the conditioned medium (CM) from hAECs promoted the survival of mesencephalic dopaminergic neurons stimulated with 1-methyl-4-phenylpyridine (MPP) and induced neurite outgrowth. Next, analysis of hAEC-CM with an antibody array of 507 soluble target proteins revealed that the levels of many neurotrophic factors, growth factors, neuronal cell adhesion molecule (NrCAM) and anti-inflammatory factors were evidently high. In addition, antibody neutralization experiments showed that many of these factors contributed to the survival and growth of dopaminergic neurons and neurite outgrowth. More importantly, we found that the anti-inflammatory factor interleukin-1 receptor antagonist (IL-1ra) also augmented the survival of dopaminergic neurons, demonstrating for the first time an anti-oxidative and anti-inflammatory role of hAECs in PD mice, which represents a novel molecular mechanism of hAECs in the treatment of PD. The molecular mechanism of hAECs recovering lesioned dopaminergic neurons and attenuating PD symptoms. First, hAECs secret many neurotrophic factors, growth factors, and neuronal cell adhesion molecule (NrCAM) which promote the growth of the damaged dopaminergic neurons and their neurites. Second, hAECs produce many anti-inflammatory factors and other factors contributing to reducing the activation of microglia and suppressing the neuroinflammation. Third, hAECs reduce the excessive ROS levels by upregulating some anti-oxidative signals.
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http://dx.doi.org/10.1007/s11481-020-09969-wDOI Listing
November 2020

Depicting an energetic chain involved in physiological responses of blue mussel Mytilus edulis coping with BDE-47 exposure.

Chemosphere 2021 Apr 25;269:128736. Epub 2020 Oct 25.

Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No.5 Yushan Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Pilot Qingdao National Laboratory for Marine Science and Technology, No 1. Wenhai Road, Qingdao, China. Electronic address:

Depiction on an energetic chain in terms of assimilation, allocation and consumption as well as the linkage between energetic alteration and physiological process was performed in blue mussel Mytilus edulis coping with tetrabromodiphenyl ether (BDE-47) based on a 21-day bioassay to shed light on the possible mechanism from energetic perspective. The filtration was hindered along with BDE-47 concentration increment and the influence of digestion was suggested according to the combination of the digestive enzymatic activities' alteration and digestive gland tissue impairment, both of which decided the energy availability reduction. Energy consumption indicated by the electron transport system activity was firstly inhibited while was greatly increased with BDE-47 increment, and the cellular energy allocation and adenylate pool were decreased simultaneously. An energetic chain was thus depicted: it tended to reduce energy absorption, elevate the energy consumption and decrease the energy metabolism with BDE-47 exposure, and M. edulis adopted the energetic strategy with variation regarding to the stressing level, suggesting as the preference switched from protein utilization to lipid utilization with the concentration increment. A consistence was observed in index of growth and survival with the change of energy allocation, inferring the energetic involvement in sustaining the viability of the mussel.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128736DOI Listing
April 2021

Modulation format-independent optical performance monitoring technique insensitive to chromatic dispersion and polarization mode dispersion using a multi-task artificial neural network.

Opt Express 2020 Oct;28(22):32331-32341

We propose and experimentally demonstrate modulation format-independent optical performance monitoring (OPM) based on a multi-task artificial neural network (MT-ANN). Optical power measurements at a series of center wavelengths adjusted using a widely tunable optical bandpass filter (OBPF) are used as the input features for a MT-ANN to simultaneously realize high-precision optical signal-to-noise ratio (OSNR) and launch power monitoring and baud rate identification (BRI). This technique is insensitive to chromatic dispersion (CD) and polarization mode dispersion (PMD). The experimental verification in a 9-channel WDM system shows that for 10 Gbaud QPSK and 32 Gbaud PDM-16QAM signals with OSNR in the range of 1-30 dB, the OSNR mean absolute error (MAE) and root mean square error (RMSE) are 0.28 dB and 0.48 dB, respectively. For launch power in the range of 0-8 dBm, the MAE and RMSE of the launch power monitoring are 0.034 dB and 0.066 dB, respectively, and the identification accuracy for both baud rates is 100%. Furthermore, this technique utilizes a single MT-ANN instead of three ANNs to realize the simultaneous monitoring of three OPM parameters, which greatly reduces the cost and complexity.
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http://dx.doi.org/10.1364/OE.402939DOI Listing
October 2020

A20-Binding Inhibitor of NF-B 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats.

Evid Based Complement Alternat Med 2020 13;2020:6980398. Epub 2020 Oct 13.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

A20-binding inhibitor of NF-B 1 (ABIN1) is an inhibitor of NF-B and exerts anti-inflammatory effect. Electroacupuncture (EA) is considered as a neuroprotective strategy by inhibiting neuroinflammatory damage after cerebral ischemia. This study was performed to explore the role of ABIN1 and investigate whether the ABIN1 is involved in the mechanism of EA in cerebral ischemia/reperfusion (I/R) rats. Male Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R) and received EA after reperfusion once a day. Lentivirus-mediated ABIN1 gene knockdown was used to detect the role of ABIN1 in neuroinflammation after I/R. ABIN1 expression, proinflammatory cytokine levels, microglial activation, neurological function, infarct volumes, and NF-B activation were assessed. ABIN1 expression was elevated in the peri-infarct cortex and was further upregulated by EA. ABIN1 knockdown increased the levels of proinflammatory cytokines and activation of microglia, worsened neurological deficits, and enlarged the infarct volume. Moreover, ABIN1 was blocked to partially reverse the neuroprotective effect of EA, and this treatment weakened the ability of EA to suppress NF-B activity. Based on these findings, ABIN1 is a potential suppressor of neuroinflammation and ABIN1 mediates the antineuroinflammatory effect of EA in cerebral I/R rats.
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http://dx.doi.org/10.1155/2020/6980398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582058PMC
October 2020

KR-12 coating of polyetheretherketone (PEEK) surface polydopamine improves osteointegration and antibacterial activity .

J Mater Chem B 2020 11;8(44):10190-10204

Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Polyetheretherketone (PEEK) is considered to be a promising bone implant material owing to its biocompatibility and elastic modulus, which is similar to that of natural bone. However, the clinical potential of PEEK is severely limited by its bioinertness, which leads to poor osseointegration, and the lack of antibacterial properties. In this study, the antimicrobial peptide, KR-12, was immobilized on the surface of PEEK implants with the assistance of polydopamine (PDA) to inhibit bacterial infection as well as to promote osteogenesis and osseointegration. Compared to unmodified PEEK, the PEEK with immobilized KR-12 showed significantly improved antibacterial activity against Staphylococcus aureus (ATCC 25923), both in vitro and in vivo. For the in vitro and in vivo evaluation of the osteogenic properties of modified PEEK, rat bone mesenchymal stem cells (rBMSCs) and a rat femoral defect model were used, respectively. The in vitro studies showed that compared to rBMSCs treated with unmodified PEEK, those treated with KR-12-coated PEEK exhibited improved adhesion, proliferation, and osteogenic differentiation. Moreover, micro-computed tomography and histological analysis suggested that the KR-12 coating promoted osteointegration in vivo in rat femurs. Taken together, these results suggest that the KR-12 coating could improve the antibacterial ability of pure or PDA-coated PEEK against Staphylococcus aureus (ATCC 25923), both in vitro and in vivo. Overall, KR-12 combined with the PDA film coating synergistically induced osteogenic effects both in vitro and in vivo. Thus, the surface-modified material, which exhibits both anti-bacterial and osteointegration properties, shows considerable potential for use as an orthopedic implant.
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http://dx.doi.org/10.1039/d0tb01899fDOI Listing
November 2020

Statin use and the risk of post-stroke seizures: A meta-analysis.

Seizure 2020 Dec 13;83:63-69. Epub 2020 Oct 13.

Department of Neurology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Purpose: The magnitude of association between statin use and post-stroke seizures (PSS) risk remains unclear. Therefore, the aim of this meta-analysis was to evaluate this issue.

Methods: We systematically searched electronic libraries, including Medline, Embase, and Cochrane databases, for relevant clinical studies. The main outcome was the risk of early PSS and the risk of post-stroke epilepsy (PSE). The pooled relative risks (RRs) and the corresponding 95% confidence intervals (CIs) were used to calculate the association between statin treatment and risks of early PSS and PSE.

Results: A total of 7 articles met our inclusion criteria and were included. For early PSS risk, statin use was associated with a lower risk of early PSS (RR 0.36, 95% CI 0.25-0.53; p < 0.001). Subgroup analyses based on the prescribing timing of statins showed that pre-stroke statin use was not associated with the risk of early PSS; post-stroke statin use was associated with a lower risk of early PSS (RR 0.37, 95% CI 0.25-0.54; p < 0.001). For PSE risk, statin use was associated with a lower risk of PSE (RR 0.62, 95% CI 0.42-0.92; p = 0.017). Further subgroup analyses based on the prescribing timing of statins indicated that pre-stroke statin use was not associated with the risk of PSE; post-stroke statin use was associated with a lower risk of PSE (RR 0.59, 95% CI 0.49-0.70; p < 0.001).

Conclusions: Statin treatment, especially the post-statin treatment, was associated with lower risks of early PSS and PSE.
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http://dx.doi.org/10.1016/j.seizure.2020.10.004DOI Listing
December 2020

Intraoperative 99mTc-MIBI-Guided Parathyroidectomy Improves Curative Effect of Parathyroidectomy, Bone Metabolism, and Bone Mineral Density.

Am Surg 2021 Mar 13;87(3):463-472. Epub 2020 Oct 13.

56694 Department of Head and Neck Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

This study aimed to compare the postoperative effects of total parathyroidectomy plus forearm transplantation and radioguided parathyroidectomy on bone metabolism and bone mineral density (BMD). From June 2013 to October 2017, 67 patients with renal secondary hyperparathyroidism (SHPT) received surgical treatment. The control group included 30 cases of classical total parathyroidectomy plus forearm transplantation for SHPT. In the experimental group, 37 patients underwent 99mTc-MIBI-guided parathyroidectomy. Demographics, parathyroid hormone (PTH) level, blood calcium level, and pathological results were compared between the 2 groups. The curative effect of parathyroidectomy and its effect on BMD were also compared. The BMDs in the L1-L4 segments and femoral neck in both groups were significantly improved after operation (all < .05). The scores of the L1-L4 segments and femoral neck in both groups were significantly improved after operation (all < .05). The improvement in the score of the L4 in the experimental group was significantly higher than that in the control group ( < .05). No significant differences in the improvement in the L1-L3 segments and femoral neck were found between the 2 groups. Both traditional total parathyroidectomy plus forearm transplantation and 99mTc-MIBI-guided parathyroidectomy can improve PTH level, blood calcium level, phosphorus level, bone metabolism, and BMD to varying degrees in patients with SHPT. Compared with the traditional surgery, 99mTc-MIBI-guided parathyroidectomy can improve blood calcium and phosphorus metabolisms, reduce PTH level, and improve the scores of L4 to a greater extent.
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http://dx.doi.org/10.1177/0003134820951467DOI Listing
March 2021

Dissymmetric interface design of SnO/TiO side-by-side bi-component nanofibers as photoanodes for dye sensitized solar cells: Facilitated electron transport and enhanced carrier separation.

J Colloid Interface Sci 2021 Feb 16;583:24-32. Epub 2020 Sep 16.

Key Laboratory for Special Function Materials & Structural Design of the Ministry of Education, and School of Physical Science & Technology, Lanzhou University, Lanzhou 730000, China.

SnO/TiO type II heterojunctions are often introduced to enhance the separation efficiency of photogenerated carriers in photoelectrochemical electrodes, while most of these heterojunctions are of core-shell structure, which often limits the synergistic effect from the two components. In this work, dissymmetric SnO/TiO side-by-side bi-component nanofibers (SBNFs) with tunable composition ratios have been prepared by a novel needleless electrospinning technique with two V-shape connected conductive channels (V-channel electrospinning). Results show that this V-channel electrospinning technique is more stable, controllable and tunable for the large-scale preparation of SBNF materials compared to the traditional electrospinning using two side-by-side metal needles. And these SnO/TiO SBNFs are dissymmetric and comprised of a tiny SnO NF (tunable diameter within 20-80 nm) and a Sn-doped TiO NF (diameter of ~ 250 nm) with a side-by-side structure. Moreover, the dye-sensitized solar cells (DSSCs) based these dissymmetric SnO/TiO SBNFs show the maximum power conversion efficiency (PCE) of 8.3%, which is 2.59 times that of the ones based on the TiO NFs. Series of analyses indicate that the enhancements in PCE could mainly be due to the improved electron transport via SnO NFs and the enhanced carrier separation via dissymmetric SnO/TiO heterojunction interface. This research will give some new insight in the preparation of SBNFs for high-performance photoelectrochemical devices.
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http://dx.doi.org/10.1016/j.jcis.2020.09.017DOI Listing
February 2021

Inhibits Tumorigenesis of Renal Cell Carcinoma by Targeting .

Cancer Biother Radiopharm 2020 Sep 11. Epub 2020 Sep 11.

Department of Nephrology, Wuhan Third Hospital, Wuhan, China.

Renal cell carcinoma (RCC) is one of the most common and malignant tumors in the urinary system. This article set out with the aim of investigating the mechanism and clinical significance of in the RCC progression. Twenty-eight (28) paired RCC tissue samples and adjacent nontumor tissue samples, as well as RCC cell lines were used to measure the expression of and protein phosphatase 1 regulatory subunit 3C () transcript by real-time quantitative PCR. The target relationship between and was predicted by TargetScan and further verified by dual-luciferase reporter gene assay and RNA pull-down assay. Cell Counting Kit-8 (CCK-8) assay and BrdU ELISA assay were implemented to measure RCC cell viability and proliferation. In addition, caspase-3 activity assay and cell adhesion assay were implemented to measure RCC cell apoptosis and adhesion. was lowly expressed both in RCC tissues and cells, while upregulated was tested in RCC tissues and cells. In addition, was validated to directly target , thereby negatively regulating . Particularly, exerted a clear inhibitory effect on the malignant phenotypes of RCC cells by binding and inhibiting . , which served as a tumor suppressor, inhibited RCC progression by targeting and downregulating .
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http://dx.doi.org/10.1089/cbr.2020.3846DOI Listing
September 2020

Anomalous Melting Point of Multicharge Ionic Liquids: Structural, Electrostatic, and Orbital Properties of [Ln(NO)] (Ln = Ce, Pr) Anions.

Inorg Chem 2020 Sep 9;59(18):13700-13708. Epub 2020 Sep 9.

College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.

Salts composed of multicharged cations/anions usually exhibit a large lattice energy and strong Coulomb force, which results in high melting points. However, an increasing number of highly charged ionic liquids exceed expectations based on conventional experience; even their melting points are much lower than those found for simple ionic liquids composed of monovalent ions. To further study this phenomenon, we studied a group of stable ionic liquids containing tricharged [Ce(NO)] and [Pr(NO)] anions. The structures for [Cmim][Ce(NO)] and [Cmim][Pr(NO)] were determined by single-crystal X-ray diffraction with triclinic and 1̅ space groups. The electrostatic potential density per unit ion surface and volume was proposed and calculated. Additionally, theoretical analysis based on Hirshfeld surface and charge decomposition was carried out to explore the intermolecular interaction and electronic structure of the lanthanide anions. The electrostatic and orbital properties were found to be more useful for understanding the melting points of highly charged salts compared with the sole use of lattice energy. The electrostatic potential density per unit ion surface and volume showed a linear relationship with the melting point of ionic liquids composed of monovalent to trivalent ions. These structure-melting point relationships will be beneficial for expounding new low-melting-point ionic liquids with a wide liquidus range.
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http://dx.doi.org/10.1021/acs.inorgchem.0c02078DOI Listing
September 2020

Simulation and experimental investigation of dust-collecting performances of different dust exhaust hoods.

J Air Waste Manag Assoc 2020 12 28;70(12):1367-1377. Epub 2020 Oct 28.

School of Chemical Engineering and Technology, China University of Mining and Technology , Xuzhou, People's Republic of China.

High-efficiency dust collection for open dust source has always been an important and difficult issue for air quality control at the workplace. This study performed simulations and experiments on three kinds of dust exhaust hoods, namely, updraft, side-draft and air-curtain exhaust hoods. Results show these three kinds of exhaust hoods varied significantly in dust-capturing efficiency. The dust-capturing efficiency of the updraft exhaust hood was the lowest (56.8%) while the air-curtain exhaust hood performed best in dust collection, with a dust-capturing efficiency of 93.8%. For a rectangular air-curtain exhaust hood with a size of 900 mm x 1200 mm, the dust-capturing efficiency first increased and gradually tended to stabilize with increasing air-curtain velocity and suction airflow rate. According to the present research results, the reasonable long-side air-curtain velocity and suction airflow rate were in the range 4 ~ 6.27 m/s and 5.4 ~ 9 m/min, respectively. Under these conditions, dust-capturing efficiency can be as high as 79.6-86.5%. : This study performed simulations and experiments on three kinds of dust exhaust hoods: updraft, side-draft and air-curtain. Simulation results show the dust-capturing efficiency of the air-curtain exhaust hood is best of 93.8%. Experimental results show under reasonable conditions, the dust-capturing efficiency of a rectangular air-curtain exhaust hood is 79.6-86.5%. This research is very meaningful, which not only has important innovation in the study of micro mechanism of dust collectors but provides powerful theoretical and experimental support for technological innovation.
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http://dx.doi.org/10.1080/10962247.2020.1813841DOI Listing
December 2020

Targeted Delivery of CXCL9 and OX40L by Mesenchymal Stem Cells Elicits Potent Antitumor Immunity.

Mol Ther 2020 12 8;28(12):2553-2563. Epub 2020 Aug 8.

State Key Laboratory of Oncogenes and Related Genes, Renji-Med-X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200127, China; Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address:

Major obstacles in immunotherapies include toxicities associated with systemic administration of therapeutic agents, as well as low tumor lymphocyte infiltration that hampers the efficacies. In this study, we report a mesenchymal stem cell (MSC)-based immunotherapeutic strategy in which MSCs specifically deliver T/natural killer (NK) cell-targeting chemokine CXCL9 and immunostimulatory factor OX40 ligand (OX40L)/tumor necrosis factor superfamily member 4 (TNFSF4) to tumor sites in syngeneic subcutaneous and azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced spontaneous colon cancer mouse models. This approach generated potent local antitumor immunity by increasing the ratios of tumor-infiltrating CD8 T and NK cells and production of antitumor cytokines and cytolytic proteins in the tumor microenvironment. Moreover, it improved the efficacy of programmed death-1 (PD-1) blockade in a syngeneic mouse model and significantly suppressed the growth of major histocompatibility complex class I (MHC class I)-deficient tumors. Our MSC-based immunotherapeutic strategy simultaneously recruits and activates immune effector cells at the tumor site, thus overcoming the problems with toxicities of systemic therapeutic agents and low lymphocyte infiltration of solid tumors.
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http://dx.doi.org/10.1016/j.ymthe.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704751PMC
December 2020

Effects of soft pellet creep feed on pre-weaning and post-weaning performance and intestinal development in piglets.

Anim Biosci 2021 Apr 24;34(4):714-723. Epub 2020 Jun 24.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.

Objective: This study aimed to determine the effects of soft pellet creep feed (SPCF) on growth performance and intestinal development in piglets.

Methods: A total of 18 sows and their litters of crossbred piglets (14±2 days, 3.73±0.72 kg) were assigned to one of three dietary groups receiving i) powder creep feed (PCF), ii) hard pellet creep feed (HPCF) or iii) SPCF during the pre-weaning period. After weaning, piglets were selected for continuous evaluation of the three diets on growth performance and intestinal health.

Results: In the pre-weaning period, the average daily feed intake and average daily dry matter intake were significantly higher in the SPCF group than the HPCF group (p<0.05). In the post-weaning and entire experimental period, the different diets had no significant effect on growth performance. At 10 d after weaning, the serum glucose concentration was lower in the SPCF group (p<0.05) than the other groups; a higher (p<0.05) villus height and lower (p<0.05) crypt depth in the jejunum were also observed in the SPCF group than the other groups; Meanwhile, in the duodenum and jejunum, the SPCF group had a higher (p<0.05) villus height to crypt depth ratio than the other groups; Furthermore, the higher (p<0.05) threshold cycle values of lactic acid bacteria and lower (p<0.05) threshold cycle values of Clostridium, Enterobacter and Escherichia coli were also observed in the SPCF group, and the sucrase and maltase activity was higher (p<0.05) in the SPCF group than the other groups in duodenum and ileum.

Conclusion: The SPCF improved pre-weaning feed intake and decreased the negative effects of weaning stress in the intestine in piglets.
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http://dx.doi.org/10.5713/ajas.20.0034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961284PMC
April 2021