Publications by authors named "Yongyan Liao"

7 Publications

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The characterization, expression and activity analysis of three superoxide dismutases in Eriocheir hepuensis under azadirachtin stress.

Fish Shellfish Immunol 2021 Oct 18;117:228-239. Epub 2021 Aug 18.

Beibu Gulf University, Qinzhou, Guangxi, 530005, PR China; School of Marine Sciences, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530005, PR China. Electronic address:

Superoxide dismutase (SOD) can effectively eliminate of excess ROS, which causes oxidative damage to lipids, proteins, and DNA. In this study, we cloned the CuZn-SOD, cMn-SOD1, and cMn-SOD2 genes in Eriocheir hepuensis, and found that the coding sequence (CDS) lengths were 627 bp, 861 bp and 1062 bp, which encoded 208, 286, and 353 amino acids, respectively. Phylogenetic analysis indicated that all SOD genes were evolutionarily conserved, while cMn-SOD2 had an extra gap (67 amino acids) in the conserved domain compared with cMn-SOD1 without huge changes in the tertiary structure of the conserved domain, suggesting that cMn-SOD2 may be a duplicate of cMn-SOD1. qRT-PCR showed that the three SOD genes were widely expressed in all the tested tissues, CuZn-SOD and cMn-SOD1 were mostly expressed in the hepatopancreas, while cMn-SOD2 was mostly expressed in thoracic ganglia. Under azadirachtin stress, the oxidation index of surviving individuals, including the T-AOC, SOD activity, and MDA contents increased in the early stage and then remained steady except for a decrease in MDA contents in the later stage. qRT-PCR showed that the three SOD genes displayed the same trends as SOD activity in surviving individuals, and the highest expressions of CuZn-SOD in the hepatopancreas, heart, and gill were 14.16, 1.41, and 30.87 times that of the corresponding control group, respectively. The changes were 1.35, 5.77 and 3.33 fold for cMn-SOD1 and 1.62, 1.71 and 1.79 fold for cMn-SOD2, respectively. However, the activity and expression of SOD genes in dead individuals were lower than that observed in surviving individuals. These results reveal that SOD plays a significant role in the defence against azadirachtin-induced oxidative stress.
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http://dx.doi.org/10.1016/j.fsi.2021.08.010DOI Listing
October 2021

Metabolic scaling: individual versus intraspecific scaling of Nile tilapia (Oreochromis niloticus).

J Comp Physiol B 2021 07 2;191(4):721-729. Epub 2021 May 2.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, China.

We examined intraspecific scaling of the resting metabolic rate (RMR) of Nile tilapia (Oreochromis niloticus) under different culture conditions and further explored the allometric relationships between organ mass (heart, liver, brain, gills, viscera, and red muscles) and blood parameters (erythrocyte size and red blood cell counts) and body mass. Oreochromis niloticus were bred in individual and group cultures. The scaling exponent of the RMR in the individual cultures was b = 0.620-0.821 (n = 30) and that in the group culture was b = 0.770 [natural logarithm (ln) RMR = 0.770 ln M - 1.107 (n = 76)]. The results of the two experimental methods were similar and were not significantly different from 0.75 (3/4), as predicted by the metabolic theory of ecology. The active and inactive organs were scaled with body mass by an exponent of 0.940 and 1.012, respectively. There was no significant relationship between the blood parameters and body mass. These results suggest that the differences in the culture methods may not have affected the allometric scaling of O. niloticus metabolism. The proportion of active and inactive organs contributed to allometric changes in the metabolic rate with body mass. Red blood cells in fish are not generally representative, and cell size can only partially explain the allometric scaling of metabolism.
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http://dx.doi.org/10.1007/s00360-021-01376-8DOI Listing
July 2021

Intestinal Tract Microbe Communities Associated with Horseshoe Crabs from Beibu Gulf, China.

Curr Microbiol 2020 Nov 14;77(11):3330-3338. Epub 2020 Sep 14.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, 535011, Guangxi, China.

Until now, there has been little research on the intestinal microbial community of horseshoe crabs. To fill this gap, we investigated the microbiome composition of the Chinese horseshoe crab, Tachypleus tridentatus, and the mangrove horseshoe crab, Carcinoscorpius rotundicauda. We sequenced the 16S rRNA gene of intestinal bacterial species and compared the microbial community structure and diversity. Next, we show that the total effective bacterial sequence was 36,865 reads, and the average annotated operational taxonomic unit (OTU) number was 240. Through hierarchical clustering analysis and principal coordinate analysis samples from two horseshoe crab species, we found that the intestinal flora of the same horseshoe crab species was relatively concentrated, while the microbiome of a different horseshoe crab species were significantly separated. Cluster analysis showed that two samples, one from Chinese horseshoe crabs and one from mangrove horseshoe crabs, had similar microbial community structure, while other samples were relatively discrete. The gut microbiota of the mangrove horseshoe crab were dominated by the phyla Tenericutes (42.71%), Firmicutes (24.27%), and Proteobacteria (20.39%), while the top three phyla in the Chinese horseshoe crab intestinal tract were Tenericutes (57.19%), Proteobacteria (22.14%), and Bacteroidetes (7.38%). To intuitively understand the similarity and overlap of the OTU composition of each group, we performed Venn diagram analysis. The two species shared 284 OTUs, accounting for 81.8% of the total. This indicates that although there is high similarity between mangrove and Chinese horseshoe crab in gastrointestinal microbial community structure, there are also some differences, which deserve further discussion.
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http://dx.doi.org/10.1007/s00284-020-02140-xDOI Listing
November 2020

sp. nov., a mangrove soil actinobacterium.

Int J Syst Evol Microbiol 2020 Mar;70(3):1800-1804

College of Marine Science, Beibu Gulf University, Qinzhou 535011, PR China.

A novel strain (SSL-25) was isolated from mangrove soil sampled at QinzhouBay, PR China. The isolate was observed to be Gram-stain-positive and to form greyish-white aerial mycelia that differentiated into straight spore chains with smooth-surfaced spores on International Project 2 medium. The cell-wall peptidoglycan was determined to contain ll-diaminopimelicacid. The cell-wall sugars were glucose and mannose. The predominant menaquinones were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The major polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and several unidentified phospholipids. The predominant cellular fatty acids were C, iso-C and summed feature 3 (Cω7/Cω6). The genome size of strain SSL-25 was 8.1 Mbp with a G+C content of 71.5 mol%. Phylogenetic analysis indicated that strain SSL-25 is closely related to NRRL 18488 (99.4 % sequence similarity). However, the digital DNA-DNA hybridization (39.8 %) and average nucleotide identity (91.3 %) values between them showed that it represents a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain SSL-25 from NRRL 18488. Therefore, based on these results, it is concluded that strain SSL-25 represents a novel species, for which the name sp. nov. is proposed. The type strain is SSL-25 (=CICC 11054=JCM33585).
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http://dx.doi.org/10.1099/ijsem.0.003974DOI Listing
March 2020

The complete mitochondrial genome of .

Mitochondrial DNA B Resour 2019 Sep 11;4(2):2942-2943. Epub 2019 Sep 11.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, China.

is a common and important component of mangrove ecosystem. In this study, the mitogenome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15,710 bp was 31.37% for A, 34.91% for T, 19.47% for G, 14.25% for C, and its GC content was 33.72%. The mitogenome was composed of 13 protein-coding genes, 22 tranfer RNAs, and 2 ribosomal RNAs. Polygenetic analysis showed that the was more close to and . We speculated that the was evolved from freshwater species.
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http://dx.doi.org/10.1080/23802359.2019.1662744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707012PMC
September 2019

The complete mitochondrial genome of (Stimpson, 1860).

Mitochondrial DNA B Resour 2019 Sep 6;4(2):2834-2835. Epub 2019 Sep 6.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, Guangxi Autonomous Regions, China.

is a common and important shrimp species in the shallow waters of Indo-West Pacific. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15968 bp was 35.16% (5614 bp) for A, 33.51% (5351 bp) for T, 11.54% (1842 bp) for G, 19.80% (3161 bp) for C, and its GC content was 31.34%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 transfer RNAs, 1 D-loop and 2 ribosomal RNAs. Polygenetic analysis showed that the was more closed to .
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http://dx.doi.org/10.1080/23802359.2019.1660279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706880PMC
September 2019

The complete mitochondrial genome of (Philippi, 1848).

Mitochondrial DNA B Resour 2019 Jul 24;4(2):2742-2743. Epub 2019 Jul 24.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, Guangxi Autonomous Regions, China.

is a common and important component of mangrove ecosystem. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15633 bp was 31.14% for A, 35.70% for T, 16.65% for G, 16.51% for C, and its GC content was 33.16%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 tranfer RNAs, and 2 ribosomal RNAs. Polygenetic analysis showed that the was more closed to than and . We may speculate that the is evolved from freshwater species.
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http://dx.doi.org/10.1080/23802359.2019.1644549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706624PMC
July 2019
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