Publications by authors named "Yongxia Zhang"

75 Publications

Comprehensive Landscape of Prognostic Significance and Immune Characteristics of Myosins in Squamous Cell Carcinoma of the Head and Neck.

J Immunol Res 2022 18;2022:5501476. Epub 2022 Apr 18.

School of Clinical Medicine, Weifang Medical University, Weifang, China.

Myosin superfamily, a large and diverse family of molecular motors important for cell motility and migration, has been illustrated to play contradictory roles during the development of several kinds of tumors. However, the function and prognostic values of MYOs in head and neck squamous cell carcinoma (HNSCC) still remain largely unknown. In the current manuscript, the expression levels and clinical data of MYOs in HNSCC were investigated by online databases, including Oncomine, GEPIA, GEO, TCGA, HPA, UALCAN, Kaplan-Meier plotter, and CancerSEA; we found that the expression levels of MYO1B, MYO5A, and MYO10 were significantly elevated in HNSCC tissues, which were also correlated with the unfavorable overall survival (OS) of the patients. Furthermore, MYO1B/MYO5A/MYO10 interacting genes were identified, and the protein-protein interaction (PPI) networks were constructed by STRING and GeneMANIA. The enrichment analysis revealed that MYO1B/MYO5A/MYO10 associated genes mainly participated in cell metastasis and EMT processes, which were also confirmed by cell functional experiments. At last, the ssGSEA method was conducted to investigate the extent of immune cell infiltration, and we found that both the expression of MYO1B/MYO5A/MYO10 were closely correlated with the infiltration of immune cells in HNSCC. These findings implied that MYO1B, MYO5A, and MYO10 as novel prognostic factors for HNSCC and provided new strategy for HNSCC treatment.
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http://dx.doi.org/10.1155/2022/5501476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038433PMC
April 2022

SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato.

Plant Biotechnol J 2022 Apr 11. Epub 2022 Apr 11.

Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China.

MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of SlMIR164A, one of the primary genes of Sly-miR164, in tomato. We show that SlMIR164A is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of SlMIR164A by CRISPR/Cas9-mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that SlMIR164A modulates fruit ripening and quality through specific target genes, SlNAM2 and SlNAM3, which control key regulators of chloroplast function and fruit ripening processes. MIR164 genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of SlMIR164A in ripening control, which will further our understanding of the actions of MIR164 family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of SlMIR164A exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture.
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http://dx.doi.org/10.1111/pbi.13824DOI Listing
April 2022

Diagnostic Value of Radiomics Analysis in Contrast-Enhanced Spectral Mammography for Identifying Triple-Negative Breast Cancer.

Front Oncol 2021 23;11:773196. Epub 2021 Dec 23.

Department of Medical Imaging, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Purpose: To evaluate the value of radiomics analysis in contrast-enhanced spectral mammography (CESM) for the identification of triple-negative breast cancer (TNBC).

Method: CESM images of 367 pathologically confirmed breast cancer patients (training set: 218, testing set: 149) were retrospectively analyzed. Cranial caudal (CC), mediolateral oblique (MLO), and combined models were built on the basis of the features extracted from subtracted images on CC, MLO, and the combination of CC and MLO, respectively, in the tumour region. The performance of the models was evaluated through receiver operating characteristic (ROC) curve analysis, the Hosmer-Lemeshow test, and decision curve analysis (DCA). The areas under ROC curves (AUCs) were compared through the DeLong test.

Results: The combined CC and MLO model had the best AUC and sensitivity of 0.90 (95% confidence interval: 0.85-0.96) and 0.97, respectively. The Hosmer-Lemeshow test yielded a non-significant statistic with of 0.59. The clinical usefulness of the combined CC and MLO model was confirmed if the threshold was between 0.02 and 0.81 in the DCA.

Conclusions: Machine learning models based on subtracted images in CESM images were valuable for distinguishing TNBC and NTNBC. The model with the combined CC and MLO features had the best performance compared with models that used CC or MLO features alone.
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http://dx.doi.org/10.3389/fonc.2021.773196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733550PMC
December 2021

The complete mitochondrial genome of (Lethierry 1885) (Hemiptera: Cicadellidae: Deltocephalinae: Chiasmini).

Mitochondrial DNA B Resour 2022 20;7(1):81-83. Epub 2021 Dec 20.

Anhui Province Key Laboratory of Integrated Pest Management on Crops, Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.

The complete mitochondrial genome of the widespread leafhopper species (Hemiptera: Cicadellidae: Deltocephalinae: Chiasmini) was obtained via next-generation sequencing. This mitochondrial genome is 14,832 bp in length with the 37 classical eukaryotic mitochondrial genes and a control region. All 13 protein-coding genes (PCGs) are initiated with ATN, except uses TTG as the start codon, and terminate with TAA or TAG with the exception of and which use a single T residue as the stop codon. Twenty-one of the 22 transfer RNA (tRNAs) genes have the typical clover-leaf structure except for . Unlike some other species of deltocephalinae, no tRNA rearrangements were detected. The monophyly of Cicadellidae and Deltocephalinae, as well as the monophyly of Chiasmini, with a sister relationship between and () is supported by Bayesian inference phylogenetic analyses based on 13 PCGs.
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http://dx.doi.org/10.1080/23802359.2021.2008834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725841PMC
December 2021

Sly-miR159 regulates fruit morphology by modulating GA biosynthesis in tomato.

Plant Biotechnol J 2022 05 28;20(5):833-845. Epub 2021 Dec 28.

Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China.

Fruit morphology is an important agronomical trait of many crops. Here, we identify Sly-miR159 as an important regulator of fruit morphology in tomato, a model species of fleshy-fruit development. We show that Sly-miR159 functions through its target SlGAMYB2 to control fruit growth. Suppression of Sly-miR159 and overexpression of SlGAMYB2 result in larger fruits with a reduced length/width ratio, while loss of function of SlGAMYB2 leads to the formation of smaller and more elongated fruits. Gibberellin (GA) is a major phytohormone that regulates fruit development in tomato. We show the Sly-miR159-SlGAMYB2 pathway controls fruit morphology by modulating GA biosynthesis. In particular, we demonstrate that Sly-miR159 promotes GA biosynthesis largely through the direct repression of the GA biosynthetic gene SlGA3ox2 by SlGAMYB2. Together, our findings reveal the action of Sly-miR159 on GA biosynthesis as a previously unidentified mechanism that controls fruit morphology in tomato. Modulating this pathway may have potential applications in tomato breeding for manipulating fruit growth and facilitating the process of fruit improvement.
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http://dx.doi.org/10.1111/pbi.13762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055814PMC
May 2022

Efficient mutagenesis targeting the gene in mice using a combination of Cas9 protein and dual gRNAs.

Am J Transl Res 2021 15;13(10):12094-12106. Epub 2021 Oct 15.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University Nanjing 210046, Jiangsu, China.

We injected mouse zygotes with combinations of Cas9 protein, mRNA, and two gRNAs targeting a single exon of type I interferon receptor () to determine the gene targeting efficiencies. Cas9 protein produced on-target mutations more efficiently than mRNA when each was used with a single gRNA, regardless of which gRNA was used. When mRNA and Cas9 protein were co-injected, the on-target efficiency could reach 97.0% when both gRNAs were used, which was higher than when either gRNA was used alone (61.3% and 75.5%, respectively; P<0.05). Co-injection of Cas9 protein with both gRNAs produced the highest on-target mutation rate of any combination (100.0%). Most on-target mutations were deletions of 2 to 113 nucleotides, and there were few off-target mutations in mutant animals. The expression intensity of IFNAR1 was reduced in heterozygous mice (IF) and almost or completely absent in homozygous null mice compared with that in wild-type mice (IF and Western blot). When both gRNAs targeting were used simultaneously with two gRNAs targeting , the on-target editing efficiency on each gene was 96.8% and 85.5%, respectively. Co-injection of dual gRNAs and Cas9 protein is an efficient approach for knockout and multi-gene editing in mice and may be applied in other animal models and breeding livestock.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581890PMC
October 2021

Research on Taproots Identification Technology in Quality Intelligent Management System.

Comput Intell Neurosci 2021 16;2021:8292535. Epub 2021 Sep 16.

School of Mechanical and Electrical Engineering, Kunming University of Science and Technology, Kunming 650500, China.

In the quality intelligent management system, the big roots and fibrous roots cannot be cut automatically because the machine cannot distinguish the taproot, big roots, and fibrous roots of , resulting in the automatic cutting mechanism unable to obtain the control trajectory coordinate reference of the tool feed. To solve this problem, this paper proposes a visual optimal network model detection method, which uses the image detection method of marking anchor frames to improve the detection accuracy. A variety of deep learning network models are modified by the TensorFlow framework, and the best training model is optimized by comparing the results of training, testing, and verification data. This model is used to automatically identify the taproots and provide the control trajectory coordinate reference for the actuator that cuts big roots and fibrous roots automatically. The experimental results show that the optimal network model studied in this paper is effective and accurate in identifying the taproots of .
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http://dx.doi.org/10.1155/2021/8292535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463189PMC
September 2021

MicroRNA‑502‑3p promotes survival in macrophages by modulating the inflammatory response by targeting ROCK1.

Mol Med Rep 2021 Nov 3;24(5). Epub 2021 Sep 3.

Emergency Department, Linyi People's Hospital, Linyi, Shandong 276034, P.R. China.

Tuberculosis (TB) is caused by infection and has the highest mortality rate of any single infectious disease worldwide. The aim of the present study was to investigate the function of microRNA (miR)‑502‑3p in ‑infected macrophages. The Gene Expression Omnibus database was used to analyze miR‑502‑3p expression in patients with TB and healthy individuals. THP‑1 and RAW 264.7 cells were transfected with miR‑502‑3p mimic, miR‑502‑3p inhibitor, pcDNA3.1‑ROCK1 or their negative controls. The expression levels of miR‑502‑3p and inflammatory cytokines were evaluated using reverse transcription‑quantitative PCR. The colony‑forming unit assay was performed to assess the survival of in macrophages, and Toll‑like receptor (TLR)4/NF‑κB signaling pathway‑associated protein expression levels were detected by western blotting. The nuclear translocation of NF‑κB p65 was detected via immunocytochemistry. TargetScan was used to predict the binding sites between miR‑502‑3p and ROCK1. The interaction between miR‑502‑3p and Rho‑associated coiled‑coil‑forming protein kinase 1 (ROCK1) was confirmed using a dual‑luciferase reporter assay; ROCK1 was demonstrated to be a direct target gene of miR‑502‑3p. Results from the present study demonstrated that miR‑502‑3p expression was significantly increased during infection in macrophages. Upregulation of miR‑502‑3p expression levels significantly enhanced the survival of intracellular . IL‑6, TNF‑α, and IL‑1β mRNA expression levels were significantly upregulated during infection but were downregulated by miR‑502‑3p overexpression. Moreover, miR‑502‑3p mimics transfection significantly downregulated TLR4/NF‑κB signaling pathway‑associated protein expression and significantly reduced nuclear transcription of NF‑κB in ‑infected macrophages. ROCK1 overexpression reversed the miR‑502‑3p inhibitory effect on cytokine production in ‑infected macrophages. In conclusion, miR‑502‑3p/ROCK1 may serve an anti‑inflammatory role and may improve the survival of within macrophages, which may provide a promising therapeutic target for TB.
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http://dx.doi.org/10.3892/mmr.2021.12393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436224PMC
November 2021

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution.

Hortic Res 2021 Sep 1;8(1):183. Epub 2021 Sep 1.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

As one of the largest families of angiosperms, the Orchidaceae family is diverse. Dendrobium represents the second largest genus of the Orchidaceae. However, an assembled high-quality genome of species in this genus is lacking. Here, we report a chromosome-scale reference genome of Dendrobium chrysotoxum, an important ornamental and medicinal orchid species. The assembled genome size of D. chrysotoxum was 1.37 Gb, with a contig N50 value of 1.54 Mb. Of the sequences, 95.75% were anchored to 19 pseudochromosomes. There were 30,044 genes predicted in the D. chrysotoxum genome. Two whole-genome polyploidization events occurred in D. chrysotoxum. In terms of the second event, whole-genome duplication (WGD) was also found to have occurred in other Orchidaceae members, which diverged mainly via gene loss immediately after the WGD event occurred; the first duplication was found to have occurred in most monocots (tau event). We identified sugar transporter (SWEET) gene family expansion, which might be related to the abundant medicinal compounds and fleshy stems of D. chrysotoxum. MADS-box genes were identified in D. chrysotoxum, as well as members of TPS and Hsp90 gene families, which are associated with resistance, which may contribute to the adaptive evolution of orchids. We also investigated the interplay among carotenoid, ABA, and ethylene biosynthesis in D. chrysotoxum to elucidate the regulatory mechanisms of the short flowering period of orchids with yellow flowers. The reference D. chrysotoxum genome will provide important insights for further research on medicinal active ingredients and breeding and enhances the understanding of orchid evolution.
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http://dx.doi.org/10.1038/s41438-021-00621-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408244PMC
September 2021

Genome-Wide Identification and Expression Analysis of Transcription Factors in L.

Int J Mol Sci 2021 Aug 17;22(16). Epub 2021 Aug 17.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.

The family of B-box (BBX) transcription factors contains one or two B-BOX domains and sometimes also features a highly conserved CCT domain, which plays important roles in plant growth, development and stress response. Nevertheless, no systematic study of the gene family in L. has been undertaken. In this study, a set of six TF family genes from was identified based on transcriptomic sequences, and clustered into three clades according to phylogenetic analysis. A transient expression analysis revealed that all six BBX proteins were localized in the nucleus. A yeast one-hybrid assay demonstrated that has transactivational activity, while , , , and have no transcriptional activation ability. The transcript abundance of in different tissues was divided into two major groups. The expression of , , and was higher in leaves, whereas and was higher in roots. The stress response patterns of six were detected under phytohormone treatments and abiotic stresses. The results of this study lay the basis for further research on the functions of BBX gene family members in plant hormone and stress responses, which will promote their application in breeding.
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http://dx.doi.org/10.3390/ijms22168793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396303PMC
August 2021

The chromosome-level Stevia genome provides insights into steviol glycoside biosynthesis.

Hortic Res 2021 Jun 1;8(1):129. Epub 2021 Jun 1.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, Jiangsu, China.

Stevia (Stevia rebaudiana Bertoni) is well known for its very sweet steviol glycosides (SGs) consisting of a common tetracyclic diterpenoid steviol backbone and a variable glycone. Steviol glycosides are 150-300 times sweeter than sucrose and are used as natural zero-calorie sweeteners. However, the most promising compounds are biosynthesized in small amounts. Based on Illumina, PacBio, and Hi-C sequencing, we constructed a chromosome-level assembly of Stevia covering 1416 Mb with a contig N50 value of 616.85 kb and a scaffold N50 value of 106.55 Mb. More than four-fifths of the Stevia genome consisted of repetitive elements. We annotated 44,143 high-confidence protein-coding genes in the high-quality genome. Genome evolution analysis suggested that Stevia and sunflower diverged ~29.4 million years ago (Mya), shortly after the whole-genome duplication (WGD) event (WGD-2, ~32.1 Mya) that occurred in their common ancestor. Comparative genomic analysis revealed that the expanded genes in Stevia were mainly enriched for biosynthesis of specialized metabolites, especially biosynthesis of terpenoid backbones, and for further oxidation and glycosylation of these compounds. We further identified all candidate genes involved in SG biosynthesis. Collectively, our current findings on the Stevia reference genome will be very helpful for dissecting the evolutionary history of Stevia and for discovering novel genes contributing to SG biosynthesis and other important agronomic traits in future breeding programs.
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http://dx.doi.org/10.1038/s41438-021-00565-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166950PMC
June 2021

Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.

Sci Rep 2021 05 10;11(1):9901. Epub 2021 May 10.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.

Iris germanica L. is a perennial herbaceous plant that has been widely cultivated worldwide and is popular for its elegant and vibrantly colorful flowers. Selection of appropriate reference genes is the prerequisite for accurate normalization of target gene expression by quantitative real-time PCR. However, to date, the most suitable reference genes for flowering stages have not been elucidated in I. germanica. In this study, eight candidate reference genes were examined for the normalization of RT-qPCR in three I. germanica cultivars, and their stability were evaluated by four different algorithms (GeNorm, NormFinder, BestKeeper, and Ref-finder). The results revealed that IgUBC and IgGAPDH were the most stable reference genes in '00246' and 'Elizabeth', and IgTUB and IgUBC showed stable expression in '2010200'. IgUBC and IgGAPDH were the most stable in all samples, while IgUBQ showed the least stability. Finally, to validate the reliability of the selected reference genes, the expression patterns of IgFT (Flowering Locus T gene) was analyzed and emphasized the importance of appropriate reference gene selection. This work presented the first systematic study of reference genes selection during flower bud development and provided guidance to research of the molecular mechanisms of flowering stages in I. germanica.
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http://dx.doi.org/10.1038/s41598-021-89100-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110784PMC
May 2021

Corrigendum to: TCP5 controls leaf margin development by regulating KNOX and BEL-like transcription factors in Arabidopsis.

J Exp Bot 2021 May;72(12):4590

Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 518055, PR China.

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http://dx.doi.org/10.1093/jxb/erab130DOI Listing
May 2021

Diagnostic nomogram based on intralesional and perilesional radiomics features and clinical factors of clinically significant prostate cancer.

J Magn Reson Imaging 2021 05;53(5):1550-1558

Department of Radiology, Yantai Yuhuangding Hospital, Qingdao University, Yantai, China.

Previous studies on the value of radiomics for diagnosing clinically significant prostate cancer (csPCa) only utilized intralesional features. However, the role of tumor microenvironment is important in tumor generation and progression. The aim of this study is to build and validate a nomogram based on perilesional and intralesional radiomics features and clinical factors for csPCa. This is a retrospective study, which included 140 patients who underwent prostate magnetic resonance imaging (MRI). This study used 3.0T T2-weighted imaging, apparent diffusion coefficient maps (derived from diffusion-weighted images), and dynamic contrast-enhanced MRI. Region of interest (ROI)s were segmented by two radiologists. Intralesional and combined radiomics signatures were built based on radiomics features extracted from intralesional and the combination of radiomics features extracted from intralesional and perilesional volumes. Serum total prostate-specific antigen level and combined radiomics signature scores were used to construct a diagnostic nomogram. Intraclass correlation efficient analysis was used to test intra- and inter-rater agreement of radiomics features. Min-max scalar was used for normalization. One-way analysis of variance or the Mann-Whitney U-test was used for univariate analysis. Receiver operating characteristic curve analysis, accuracy, balanced accuracy, and F1-score were used to evaluate radiomics signatures and the nomogram. Also, the nomogram was evaluated using decision curve analysis in testing cohort. Delong test was used to compare area under the curves (AUCs). Statistical significance was set at p < 0.05. In testing cohort, AUC, accuracy, balanced accuracy, and F1-score of combined radiomics signature (0.94, 0.83, 0.80, and 0.87, respectively) were all higher than that of intralesional radiomics signature (0.90, 0.77, 0.74, and 0.83, respectively). The difference between AUCs was insignificant (p of 0.19). AUC, accuracy, balanced accuracy, and F1-score of the nomogram were 0.96, 0.94, 0.95, and 0.95, respectively. Nomogram was clinically useful when threshold probability of a patient is higher than 0.06. Perilesional radiomics features improved the discrimination ability of the radiomics signature. Diagnostic nomogram had a good performance. LEVEL OF EVIDENCE: 3. TECHNICAL EFFICACY STAGE: 2.
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http://dx.doi.org/10.1002/jmri.27486DOI Listing
May 2021

Giant epidermal inclusion cyst with infection arising within the breast parenchyma: a case report.

J Int Med Res 2021 Mar;49(3):300060521997671

Department of Medical Imaging, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, P R China.

Epidermal inclusion cysts (EICs) of the breast develop in the deep breast parenchyma, and they are very rare. Only about 10 cases have been reported in the English-language literature to date. In this report, we present a rare case of a giant EIC with infection arising within the deep breast parenchyma. Unlike a typical EIC of the breast, the EIC in the present case was a cystic and solid lesion containing a large amount of liquid within the cyst and popcorn-like calcification in the wall. In this report, we describe the contrast-enhanced spectral mammography (CESM), ultrasonography, and computed tomography findings and provide a reference for the diagnosis of EICs. To the best of our knowledge, this is the first report of the CESM findings of an EIC. Our case illustrates that CESM has excellent performance similar to that of magnetic resonance imaging and is much more effective than conventional digital mammography. Additionally, our case indicates that precise correlation of CESM with ultrasonography findings contributes to the diagnosis of EICs. This rare case with multiple imaging findings will increase the awareness of EICs in the breast parenchyma.
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http://dx.doi.org/10.1177/0300060521997671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166397PMC
March 2021

Coaxial [email protected] Carbon Nanofibers Derived Hierarchical Electrodes for Efficient H Production Urea Electrolysis.

ACS Appl Mater Interfaces 2021 Jan 13;13(3):3937-3948. Epub 2021 Jan 13.

Key Laboratory of Micro-Systems and Microstructures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin 150080, P. R. China.

Electrochemical water splitting into hydrogen is a promising strategy for hydrogen production powered by solar energy. However, the cell voltage of an electrolyzer is still too high for practical application, which is mainly limited by the sluggish oxygen evolution reaction process. To this end, hybrid water electrolyzers have drawn tremendous attention. Herein, coaxial Ni/[email protected] nanofibers are directly grown on nickel foam (NF), which is highly active for hydrogen evolution reaction. Meanwhile, the [email protected] nanofibers on NF prepared in an Ar atmosphere display superior urea oxidation reaction performance to previously reported catalysts. The cell voltage is about 1.50 V in urea electrolysis to deliver a current density of 20 mA cm, lower than that of a traditional water electrolyzer (1.82 V). The current density is around 77% relative to its initial value of 20 mA cm after 20 h, superior to Pt/C|Ir/C-based urea electrolysis (14%). It is found that the synergistic effect between metallic Ni and NiS, as well as the interfacial effect between metal centers and N-doped carbon, favors the initial dissociation of HO and the adsorption/desorption of H* with thermal neutral Gibbs free energy. Meanwhile, the in-situ generated NiOOH on the outer surface of NiS possessed lower electrochemical activation energy for urea decomposition. Meanwhile, the abundant oxygen vacancies in electrodes could expose more active sites for the adsorption of intermediates, including H* and OOH*. It is also found that the hierarchical nanostructure of densely packed nanowires provides ideal electronic and ionic transport paths for fast electrocatalytic kinetics. The present work indicated that the modulation of compositions and hierarchical nanostructure is effective to prepare efficient catalysts for H production urea electrolysis.
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http://dx.doi.org/10.1021/acsami.0c19117DOI Listing
January 2021

H19/miR-107/HMGB1 axis sensitizes laryngeal squamous cell carcinoma to cisplatin by suppressing autophagy in vitro and in vivo.

Cell Biol Int 2021 Mar 31;45(3):674-685. Epub 2020 Dec 31.

Department of Otolaryngology Head and Neck Surgery, Hainan Branch of Chinese PLA General Hospital, Sanya, Hainan, China.

Laryngeal squamous cell carcinoma (LSCC) is the most common malignant tumor, which occurs in the head and neck. Current treatments for LSCC are all largely weakened by increasing drug resistance. Our study aimed to investigate the effects of long noncoding RNA (lncRNA) H19 on drug resistance in LSCC. In our study, we found that the level of H19 was sharply upregulated in LSCC tissues and drug-resistant cells compared with the control. Besides, the expression of high-mobility group B1 (HMGB1) was elevated, and microRNA107 (miR-107) was suppressed in drug-resistant cells compared with the control. Further study revealed that the interference of H19 by short hairpin RNA (shRNA) effectively suppressed high autophagy level and obvious drug resistance in drug-resistant cells. Besides that, miR-107 was predicted as a target of H19 and inhibiting effects of H19 shRNA on autophagy and drug resistance were both reversed by miR-107 inhibitor. Moreover, HMGB1 was predicted as a target of miR-107 in LSCC cells and knockdown of HMGB1 was able to suppress autophagy and drug resistance in LSCC cells. In addition, our investigation demonstrated that H19 shRNA exerted an inhibiting effect on autophagy and drug resistance by downregulating HMGB1 by targeting miR-107. Finally, the in vivo experiment revealed that LV-H19 shRNA strongly suppressed drug resistance compared with the usage of cisplatin individually. Taken together, our research indicated an H19-miR-107-HMGB1 axis in regulating the autophagy-induced drug resistance in LSCC in vitro and in vivo, providing novel targets for molecular-targeted therapy and broadening the research for LSCC.
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http://dx.doi.org/10.1002/cbin.11520DOI Listing
March 2021

TCP5 controls leaf margin development by regulating KNOX and BEL-like transcription factors in Arabidopsis.

J Exp Bot 2021 02;72(5):1809-1821

Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, PR China.

Development of leaf margins is an important process in leaf morphogenesis. CIN-clade TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF) transcription factors are known to have redundant roles in specifying leaf margins, but the specific mechanisms through which individual TCP genes function remain elusive. In this study, we report that the CIN-TCP gene TCP5 is involved in repressing the initiation and outgrowth of leaf serrations by activating two key regulators of margin development, the Class II KNOX factor KNAT3 and BEL-like SAW1. Specifically, TCP5 directly promotes the transcription of KNAT3 and indirectly activates the expression of SAW1. We also show that TCP5 regulates KNAT3 and SAW1 in a temporal- and spatial- specific manner that is largely in accordance with the progress of formation of serrations. This regulation might serve as a key mechanism in patterning margin morphogenesis and in sculpting the final form of the leaf.
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http://dx.doi.org/10.1093/jxb/eraa569DOI Listing
February 2021

Identification of GH1 gene family fgt members in Stevia rebaudiana and their expression when grown in darkness.

Mol Biol Rep 2020 Nov 24;47(11):8739-8746. Epub 2020 Oct 24.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.

Stevia rebaudiana Bertoni is an important economic crop that is well known for its secondary metabolites, steviol glycosides (SGs), found in leaves. Because the enzymes of deglycosylation (glycoside hydrolases) play important roles in SGs biosynthetic processes, our study is focused on the functions of β-glucosidases in SGs catabolism in stevia. We cloned and characterized 19 stevia GH1 genes based on transcriptomic sequences. The 19 genes were divided into five putative subfamilies in Arabidopsis. Conserved motifs in the SrGH1 proteins were analysed using the online motif-based sequence analysis tool, MEME. Most of the identified proteins contain the conserved 'TFNEP' motif (contains the catalytic acid/base) and 'ITENG' motif (contains the catalytic nucleophile). Furthermore, the steviol glycoside content and expression of these 19 genes were characterized under constant darkness. The dark treatment lowered the steviol glycoside content significantly, while SrBGLU16 responded to darkness and was markedly upregulated. This study is the first transcriptome-wide analysis of the GH1 family in Stevia rebaudiana. The sequences of 19 SrGH1 members and their expression when grown in darkness were characterized. Among the 19 genes, SrBGLU16 was markedly upregulated by darkness. Thus, we identified SrBGLU16 for further investigation as a possible steviol glycoside beta-glucosidase.
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http://dx.doi.org/10.1007/s11033-020-05920-7DOI Listing
November 2020

Plant U-box E3 ligases PUB25 and PUB26 control organ growth in Arabidopsis.

New Phytol 2021 01 18;229(1):403-413. Epub 2020 Sep 18.

Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, 518071, China.

Plant organs often grow into a genetically determined size and shape. How organ growth is finely regulated to achieve a well defined pattern is a fascinating, but largely unresolved, question in plant research. We utilised the Arabidopsis petal to study the genetic control of plant organ growth, and identify two closely related U-box E3 ligases PUB25 and PUB26 as important growth regulators by screening the targets of the petal-specific growth-promoting transcription factor RABBIT EARS (RBE). We showed that PUB25 is directly controlled by RBE in petal development in a spatial- and temporal-specific manner and acts as a major target to mediate RBE's function in petal growth. We also showed that PUB25 and PUB26 repress petal growth by restricting the period of cell proliferation, and their regulation appears to be independent of other plant E3 ligase genes implicated in growth control. PUB25 and PUB26 are among the first U-box E3 ligases shown to function in plant growth control. Furthermore, as they were also found to play a vital role in plant stress responses, PUB25 and PUB26 may act as a key hub to integrate developmental and environmental signals for balancing growth and defence in plants.
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http://dx.doi.org/10.1111/nph.16885DOI Listing
January 2021

The Clinical and Molecular Characterization of Gastric Cancer Patients in Qinghai-Tibetan Plateau.

Front Oncol 2020 30;10:1033. Epub 2020 Jun 30.

Institute of Sanjiangyuan National Park, Chinese Academy of Sciences, Xining, China.

Gastric cancer was the fifth most common malignancy and the third deadliest cancer (738,000 deaths in 2018) in the world. The analysis of its molecular characteristics has been complicated by histological and intratumor heterogeneity. Furthermore, the previous studies indicate that the incidence of gastric cancer shows wide geographical variation. As the largest and highest region in China, Qinghai-Tibetan Plateau (QTP) is one of the important global biodiversity hotspots. Here, to better understand the mechanism of gastric cancer and offer the targeted therapeutic strategies specially designed for patients in QTP, we collect tumor and blood samples from 30 primary gastric adenocarcinoma cancer patients at Qinghai Provincial People's Hospital. We discuss the clinical and molecular characteristics for these patients that have been ascribed to the unique features in this place, including high altitude (the average height above sea level is around 4,000 m), multi-ethnic groups, and the specific ways of life or habits (such as eating too much beef and mutton, have alcohol and cigarette problem, et al.). By comparing with the western gastric cancer patients collected from TCGA data portal, some unique characteristics for patients in QTP are suggested. They include high incidence in younger people, most of tumor are located in body, most of SNP are detected in chromosome 7, and the very different molecular atlas in minor ethnic groups and Han Chinese. These characteristics will provide the unprecedented opportunity to increase the efficacy for diagnosis and prognosis of gastric cancer in QTP. Furthermore, to suggest the targeted therapeutics specially designed for these 30 patients, an adapted kernel-based learning model and a compilation of pharmacogenomics data of 462 patient-derived tumor cells (PDCs) that illustrate the diverse genetic and molecular backgrounds of cancer patients, were introduced. In conclusion, our study offers a big opportunity to better understand the mechanism of gastric cancer in QTP and guide the optimal patient-tailored therapy for them.
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http://dx.doi.org/10.3389/fonc.2020.01033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339979PMC
June 2020

Oxygen Vacancy-rich Ni/[email protected] Nanosheets with Schottky Heterointerface for Efficient Urea Oxidation Reaction.

ChemSusChem 2020 Sep 4;13(18):5004-5014. Epub 2020 Aug 4.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, P. R. China.

H production via electrocatalytic water splitting is greatly hindered by the sluggish oxygen evolution reaction (OER). The urea oxidation reaction (UOR) draws specific attention not only because of its lower theoretical voltage of 0.37 V compared with OER (1.23 V), but also for treating sewage water. Herein, Ni/NiO nanosheets with an ultrathin N-doped C layer containing a Schottky Ni and NiO heterointerface is constructed. Because of the self-driven charge redistribution at the heterointerface, janus charge domains are successfully created to drive the cleavage of urea molecules. Meanwhile, the synergistic effect between N-doped C and Ni/NiO restrains the deactivation of active sites in alkaline solution. The catalyst displays 1.35 V for UOR at 10 mA/cm , 0.27 V lower than that of OER. The final potential increase is only 2 mV after long-term stability test of 12 h for UOR, much smaller than the uncoated sample (38 mV). The present work shows that C-coated transition metal nanomaterials with oxygen vacancies and a Schottky heterointerface are promising candidates for simultaneously boosting UOR with both high activity and long-term stability.
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http://dx.doi.org/10.1002/cssc.202001185DOI Listing
September 2020

Quantitative proteomic analysis reveals complex regulatory and metabolic response of Iris lactea Pall. var. chinensis to cadmium toxicity.

J Hazard Mater 2020 12 11;400:123165. Epub 2020 Jun 11.

Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, MA 01003, USA. Electronic address:

Cadmium pollution has become a serious environmental problem. Iris lactea var. chinensis showed strong Cd tolerance and accumulation ability, which has significant potential to be applied for the phytoremediation of Cd-contaminated soil. However, the lack of molecular information on the mechanism of I. lactea response to Cd limited the improvement of phytoremediation efficiency. In this study, label-free proteomics analysis of Cd response in I. lactea showed that there were 163 and 196 differentially expressed proteins (DEPs) in the shoots and roots, respectively. Bioinformatics analysis indicated the DEPs responding to Cd stress mainly involved in signal transduction, ion transport, redox etc., and participate in the pathway of amino acid biosynthesis, lignin biosynthesis, glycerolipid metabolism and glutathione metabolism. Besides, differential expression of seven DEPs was validated via gene expression analysis. Finally, we found that a Cd-induced mannose-specific lectin (IlMSL) from I. lactea enhanced the Cd sensitivity and increased Cd accumulation in yeast. The results of this study will enhance our understanding of the molecular mechanism of Cd tolerance and accumulation in I. lactea and ultimately provide valuable resources for using Cd tolerant genes for developing efficient strategies for phytoremediation of Cd-contaminated soils or limiting Cd accumulation in food crops.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123165DOI Listing
December 2020

Electronic coupling between molybdenum disulfide and gold nanoparticles to enhance the peroxidase activity for the colorimetric immunoassays of hydrogen peroxide and cancer cells.

J Colloid Interface Sci 2020 Oct 6;578:366-378. Epub 2020 Jun 6.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No. 92 West Dazhi Street, Nan Gang District, Harbin 150001, People's Republic of China; Key Laboratory of Microsystems and Microstructures Manufacturing, Harbin Institute of Technology, No.2 Yikuang Street, Nan Gang District, Harbin 150080, People's Republic of China; Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya str. Moscow 119991, Russian Federation. Electronic address:

Peroxidase nanoenzymes exhibit a specific affinity toward substrates, thereby demonstrating application potential for realizing the colorimetric immunoassays of hydrogen peroxide (HO), which can be further used as a probe for imaging cancer cells. To enhance the intrinsic peroxidase activity of molybdenum sulfide (MoS) nanomaterials, gold (Au) nanoparticles with an average diameter of approximately 2.1 nm were modified on a MoS/carbon surface (denoted as MoS/C-Au) via ascorbic acid reduction. MoS/C-Au can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to generate a blue oxidation product in the presence of HO; this product exhibits peroxidase-like activities, superior to those of most existing MoS-based nanoenzymes. Furthermore, MoS/C-Au exhibits a high detection capability for HO in the range of 1 × 10 to 2 × 10 mol/L (R = 0.99), and the lowest detection limit is 1.82 µmol/L in a sodium acetate and acetic acid buffer solution. Steady state kinetics studies indicate that the catalytic mechanism is consistent with the ping-pong mechanism. Given its strong absorption peak at 652 nm in the visible region, MoS/C-Au can be used to image cancer cells due to the enhanced permeability and retention effect. Our findings demonstrate that the synergistic electronic coupling between multiple components can enhance the peroxidase activity, which can facilitate the development of an effective, facile, and reliable method to perform colorimetric immunoassays of HO and cancer cells.
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http://dx.doi.org/10.1016/j.jcis.2020.06.001DOI Listing
October 2020

A beta-glucosidase gene from Stevia rebaudiana may be involved in the steviol glycosides catabolic pathway.

Mol Biol Rep 2020 May 20;47(5):3577-3584. Epub 2020 Apr 20.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.

We herein report the preparation of a full-length raucaffricine-O-beta-D-glucosidase gene of stevia rebaudiana Bertoni (named SrRG1, GenBank accession number MK920450). Sequence analysis indicated SrRG1 consists of a 1650 bp open reading frame encoding a protein of 549 amino acids. Its deduced amino acid sequence showed a high identity of 82% with a raucaffricine-O-beta-D-glucosidase from H. annuus of glycoside hydrolase family 1. The expression pattern analyzed by real-time quantitative PCR showed no significant difference among different tissues, developmental stages, and cultivars under normal growth conditions. Furthermore, the gene function of SrRG1 was preliminarily studied by agrobacterium-mediated transformation on instantaneous expression. In the test of agrobacterium-mediated transformation on instantaneous expression, it was observed that overexpression of SrRG1 increased the accumulation of steviol content and decreased the major components and total SGs contents. Such results demonstrated that SrRG1 may participate in the steviol glycosides catabolic pathway. However, the effect of silencing construct infiltration on steviol and SGs content was not significant and its expression pattern was constitutive, which most probably, attributed the hydrolysis of SGs to the secondary activity of SrRG1. This study firstly identified the bate-glucosidase in stevia and advances our understanding of steviol glycosides hydrolyzation.
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http://dx.doi.org/10.1007/s11033-020-05450-2DOI Listing
May 2020

FeO hard templating to assemble highly wrinkled graphene sheets into hierarchical porous film for compact capacitive energy storage.

RSC Adv 2019 Jun 27;9(35):20107-20112. Epub 2019 Jun 27.

School of Material and Chemical Engineering, Zhengzhou University of Light Industry Zhengzhou 450001 PR China

Highly wrinkled graphene film (HWGF) with high packing density was synthesized by combining an electrostatically self-assembling process, a vacuum filtration-induced film assembling process and capillary compression. FeO nanoparticles were used as a low-cost and environment-friendly hard template. Hierarchical porosity and high packing density were achieved with the aid of capillary compression in the presence of FeO nanoparticles. This strategy enables integration of highly wrinkled graphene sheets to form highly compact carbon electrodes with a continuous ion transport network. The generated HWGF exhibited a high packing density of 1.53 g cm, a high specific surface area of 383 m g and a hierarchically porous structure. The HWGF delivered a high capacitance of 242 F g and 370 F cm at 0.2 A g in 6 M KOH aqueous electrolyte system with excellent rate capability (202 F g and 309 F cm retained at 20 A g). The capacity retention rate reached 97% after 10 000 cycles at 1 A g. The HWGF-based supercapacitor exhibited a high energy density of 17 W h kg at the power density of 49 W kg. Such high capacitive performances could be attributed to the highly dense but porous graphene assemblies composed of highly wrinkled graphene sheets.
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http://dx.doi.org/10.1039/c9ra02132aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065504PMC
June 2019

Dosimetry comparison between a 3D printed minimally invasive guidance template and free implantation in the brachytherapy treatment of postoperative recurrent cervical carcinoma.

Cancer Manag Res 2019 30;11:5013-5018. Epub 2019 May 30.

Department of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin, People's Republic of China.

This study aimed to investigate the dosimetry difference between a 3D printed minimally invasive guidance template and conventional free implantation in brachytherapy of postoperative recurrent cervical carcinoma under the guidance of computed tomography (CT). A total of 21 cases of patients with recurrent cervical cancer after operation were enrolled from January 2017 to June 2018. After external irradiation treatment in 1.8-Gy fractions to 45 Gy, patients were randomly divided into two groups to receive brachytherapy: 11 cases were assisted by a 3D-printed minimally invasive guidance template, and the other 10 cases were free implantation. In the template group, needles were inserted according to the main guide channel of the template commissioned in medical photosensitive resin, while patients in the other group were treated with bare hands under the guidance of CT, which was used in both groups to adjust the position and depth of the implant needles. After transmission of the CT images into the Oncentra® Brachy TPS system, the target organs and organs at risk were delineated for further treatment. The D value of the high-risk clinical target volume in the template group was 6.30±0.21 Gy while that in the other group was 6.07±0.32 Gy (<0.05). In addition, the D2cm (illuminated dose of 2 cm of organ at risk) value of the bladder, rectum, sigmoid colon, and bowel was significantly decreased in the template group as compared to the free group (<0.05). The number of needles used for each treatment in the template group was 5.71±1.82, while that for the free injection group was 7.78±2.35 (<0.05). Compared with conventional free implantation, the 3D printed minimally invasive guidance template-assisted treatment has an obvious dosimetry advantage in the treatment of postoperative recurrent cervical carcinoma, with shorter time of implantation and better repeatability.
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http://dx.doi.org/10.2147/CMAR.S195829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6551446PMC
May 2019

Evaluation of microRNA expression profiling in highly metastatic laryngocarcinoma cells.

Acta Otolaryngol 2018 Dec 31;138(12):1105-1111. Epub 2019 Jan 31.

f Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , China.

Background: Until now, little is known about the role of miRNAs in the invasion and metastasis of Laryngeal squamous cell carcinoma (LSCC).

Objectives: This study aimed to explore the relationship between microRNA and the invasion and metastasis of LSCC.

Material And Methods: The highly metastatic laryngocarcinoma cells were obtained from the established animal model with spontaneous lymph node metastasis of LSCC in our previous study. MicroRNA expression profiling and bioinformatic analysis were performed to analyze the microRNA expression changes in the highly metastatic laryngocarcinoma cells and the parental tumor cells (HEP-2). RT-PCR was performed for further validation of the result of microarray.

Results: A total of 40 microRNAs were found to be significantly altered in the highly metastatic laryngocarcinoma cells compared to controls. Bioinformatic analysis identified that 19 key microRNAs might involve in LSCC development. Moreover, RT-PCR confirmed that miR-25, miR-100, miR-125b-5p and let-7g were differentially expressed in different laryngocarcinoma cells and human tumor specimens.

Conclusions And Significance: Our findings suggest that microRNA play an important role in the invasion and metastasis of LSCC, and provide the clues for studying the function of microRNA as well as opportunities to analyze the complex molecular abnormalities driving LSCC progression.
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http://dx.doi.org/10.1080/00016489.2018.1508887DOI Listing
December 2018

Facile fabrication of hierarchical film composed of Co(OH)@Carbon nanotube core/sheath nanocables and its capacitive performance.

RSC Adv 2018 Nov 15;8(67):38550-38555. Epub 2018 Nov 15.

School of Material and Chemical Engineering, Zhengzhou University of Light Industry Zhengzhou 450001 PR China

A hierarchical film composed of Co(OH)@carbon nanotube (CNT) core/sheath nanocables (CCNF) was generated a simple and rapid electrophoretic deposition method. It is found that the Co(OH) sheath was uniformly anchored on the surface of conductive CNT core. The Co(OH) sheath, with a thickness of ∼20 nm, was composed of numerous very tiny nanoparticles. Such a unique nanostructure endows the CCNF with a high surface area of 126 m g and a hierarchical porosity, resulting in a large accessible surface area for redox activity. As expected, the CCNF exhibits high specific capacitance and excellent rate performance. Its specific capacitance reached 1215 F g under a low current density of 1 A g and was maintained at 832 F g when the current density was increased 20 times to 20 A g. A high capacitance retention of 99.3% was achieved after 10 000 cycles at 1 A g. Such intriguing capacitive behavior is attributed to the synergistic effect of the CNT core and the Co(OH) sheath.
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http://dx.doi.org/10.1039/c8ra07031hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090639PMC
November 2018

Transcriptome analysis reveals complex response of the medicinal/ornamental halophyte Iris halophila Pall. to high environmental salinity.

Ecotoxicol Environ Saf 2018 Dec 7;165:250-260. Epub 2018 Sep 7.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China. Electronic address:

The remediation and subsequent use of saline-alkaline land are of great significance to ecological environment construction and sustainable agricultural development. Iris halophila Pall. is a salt-tolerant medicinal and ornamental plant, which has good application prospects in the ecological construction of saline-alkaline land; therefore, study of the molecular mechanisms of salt tolerance in I. halophila has important theoretical and practical value. To evaluate the molecular mechanism of the response of I. halophila to salt toxicity, I. halophila seedlings were treated with salt (300 mM NaCl) and subjected to deep RNA sequencing. The clean reads were obtained and assembled into 297,188 unigenes. Among them, 1120 and 100 salt-responsive genes were identified in I. halophila shoots and roots, respectively. Among them, the key flavonoid and lignin biosynthetic genes, hormone signaling genes, sodium/potassium ion transporter genes, and transcription factors were analyzed and summarized. Quantitative reverse-transcription PCR analysis strengthened the reliability of the RNA sequencing results. This work provides an overview of the transcriptomic responses to salt toxicity in I. halophila and identifies the responsive genes that may contribute to its reduced salt toxicity. These results lay an important foundation for further study of the molecular mechanisms of salt tolerance in I. halophila and related species.
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http://dx.doi.org/10.1016/j.ecoenv.2018.09.003DOI Listing
December 2018
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