Publications by authors named "Yongxia Wang"

73 Publications

The role of Nrf2 pathway in alleviating fluorine-induced apoptosis by different selenium sources in the chicken duodenum and jejunum.

Ecotoxicol Environ Saf 2021 Aug 27;224:112708. Epub 2021 Aug 27.

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology • College of Veterinary Medicine, Zhejiang A & F University, Linan 311300, China. Electronic address:

In order to evaluate the alleviative effects and molecular mechanisms of sodium selenite (SS) and selenomethionine (SM) on excessive apoptosis induced by high fluorine (HF) in the duodenum and jejunum of broilers, 720 1 day old Lingnan Yellow broilers were randomly divided into 4 groups (each group assigned 180 chickens with 6 replicates) and offered either a control diet or test diets (800 mg/kg F, HF group; 800 mg/kg F + 0.15 mg selenium (Se)/kg as SS (SS group) or SM (SM group)) for 50 days. High F intake significantly increased (P < 0.05) apoptosis rates of duodenum and jejunum by inducing oxidative stress and leading to mitochondrial damage. Selenomethionine supplementation effectively alleviated mitochondrial damage and severe apoptosis of duodenum and jejunum caused by HF through decreasing oxidative stress parameters. Selenomethionine added group significantly increased (P < 0.05) nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA and nuclear Nrf2 protein levels as well as Nrf2 downstream antioxidant enzymes expressions in the duodenum and jejunum when compared with the HF group. Selenomethionine was superior to SS in activating the Nrf2 pathway and reducing the apoptosis rate of duodenum. It was concluded that dietary SM supplementation could ameliorate F-induced excessive apoptosis by inducing the Nrf2 pathway. Our findings will bring a promising tactics for the utilization of SM as an efficient antioxidant additive for reducing the intestinal damage caused by fluorosis in poultry.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112708DOI Listing
August 2021

A universal CRISPR/Cas12a nucleic acid sensing platform based on proximity extension and transcription-unleashed self-supply crRNA.

Anal Chim Acta 2021 Sep 14;1176:338755. Epub 2021 Jun 14.

Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan, 571199, China. Electronic address:

The extraordinary genome-editing tool CRISPR/Cas12a has also been utilized as a powerful sensing technology owing to its highly-specificity and isothermal signal amplification. Nevertheless, the widespread application of Cas12a-based sensing methods in nucleic acid detection is limited by the targeting range and high undesired background. Herein, we established a universal Cas12a-based nucleic acid sensing strategy by using proximity extension and transcription-unleashed self-suppling of crRNA. The target was recognized and bound to a pair of adjacent probes, and then triggered the proximity-induced primer extension and transcription amplification to produce numerous crRNAs. The amplified abundant crRNAs assembled with Cas12a and dsDNA activators containing PAM to form a ternary complex, which trans-cleaved ssDNA-FQ reporters continuously to generate a strong fluorescent signal. Thus, the cascade enzymatic amplification was performed and subsequently applied for detecting target DNA down to 41.7 amol with a low nonspecific background. The application of this strategy in RNA detection has also been demonstrated, and it is expected to provide a universal and sensitive sensing platform for molecular diagnosis applications.
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http://dx.doi.org/10.1016/j.aca.2021.338755DOI Listing
September 2021

Cytokines and chemokines expression pattern in herpes simplex virus type-1 encephalitis.

Neurosci Lett 2021 Oct 12;763:136170. Epub 2021 Aug 12.

School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, China. Electronic address:

To explore the differently expressed cytokines and chemokines to understand the pathways that lead to herpes simplex encephalitis (HSE). Mice in the experimental group were inoculated intracranially with HSV-1. A high-throughput cytokine chip assay was employed to assess the expression of cytokines/chemokines in the mice brain. GO, KEGG, and PPIs analyses were used to investigate the biological process (BP), pathways and interaction network of the differently expressed proteins (DEPs) in HSE. 13 DEPs and various proteins-related signal pathways were identified in HSE, including three new factors (IL-1α, MIP-1γ, and sTNF RI). The proteins were mainly implicated in leukocyte activation and chemotaxis. Additionally, the DEPs constituted a pivotal protein interaction network where IL-6 might be a mediator. 13 DEPs and a series of related signal pathways were associated with the pathophysiological mechanisms responsible for HSE. IL-6 might be a key mediator in the inflammatory responses to the disease.
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http://dx.doi.org/10.1016/j.neulet.2021.136170DOI Listing
October 2021

The Saggy Ink Cap Medicinal Mushroom, Coprinus comatus (Agaricomycetes), Protein Attenuates Acute Alcoholic Liver Injury in Association with Changes in the Gut Microbiota of Mice.

Int J Med Mushrooms 2021 ;23(5):91-100

School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China.

This study aims to investigate the hepatoprotective effects of Coprinus comatus protein (CCP) in a mouse model of acute alcoholic liver injury by regulating gut microbiota dysbiosis. Mice were divided into four groups, including the control group (CG), alcohol group (AG), biphenyldicarboxylate group (BG), and protein group (PG). The results showed that alcohol can increase the liver organ index, which could be adjusted by CCP. At the same time, analysis of serum biochemical indexes (alanine aminotransferase, aspartate aminotransferase) and liver oxidative stress levels (glutathione) revealed that CCP significantly alleviated alcohol-induced hepatic inflammation. Sequencing of 16S rRNA showed that gut microbiota composition was changed significantly by alcohol treatment. However, CCP could mitigate dysbiosis of gut microbiota, such as increasing the proportion of Muribaculaceae, Lachnospiraceae, and Lactobacillaceae and reducing the proportion of Burkholderiaceae, Deferribacteraceae, Enterococcaceae, and Enterobacteriaceae. In conclusion, CCP can maintain gut microbiota stability to improve liver injury and is potentially a good candidate for dietary supplements against acute alcoholic liver injury.
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http://dx.doi.org/10.1615/IntJMedMushrooms.2021038229DOI Listing
January 2021

Development of a TaqMan-based real-time assay for the specific detection of canine astrovirus.

J Virol Methods 2021 Oct 28;296:114247. Epub 2021 Jul 28.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China. Electronic address:

In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method to detect canine astrovirus in clinical samples was developed. Primers and probes were designed to target conserved regions of the complete viral genome sequence. The results showed that the proposed method can detect a minimum of 10 copy numbers. No cross-reactivity with other canine and feline viruses was observed. The coefficient of variation was <5%. Evaluation of the clinical samples showed that quantitative PCR had a 5.26 % higher positive detection rate than conventional PCR. These results indicate that the method developed in this study is highly reliable and suitable for veterinary clinical diagnosis and epidemiological investigations.
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http://dx.doi.org/10.1016/j.jviromet.2021.114247DOI Listing
October 2021

Exocellular polysaccharides extracted from mangrove fungus Paecilomyces Lilacinuson present anti-HSV-1 activity in mice.

J Virol Methods 2021 Jul 27;297:114246. Epub 2021 Jul 27.

Key Laboratory of Tropical Translational Medicine of Hainan Medical University, Ministry of Education, Haikou, Hainan, 571199, China. Electronic address:

This study examined the anti-HSV-1 activity of EPS extracts isolated from mangrove fungus Paecilomyces Lilacinuson after intraperitoneal administration in mice. Mice were experimentally infected with HSV-1 intracranially and treated intraperitoneally with three different doses of EPS extract (6 g/Kg, 8 g/Kg, and 10 g/Kg) for 7 days. One group of 15 mice was infected with HSV-1 but did not receive any treatment, while another group of 15 mice was mock-infected to remain a control group. Animals were observed twice a day for 14 days after virus infection, searching for clinical signs of weight loss, piloerection, isolation, or retardation movement. Compared with the mock-infected group, mortality was significantly increased (p < 0.05) in the virus-infected group and the groups that received 6 g/Kg and 8 g/Kg EPS extract. Interestingly, no significant differences in mortality were found between the 10 g/Kg EPS extract and the mock-infected group. Mortality in the 10 g/Kg EPS extract group was substantially improved compared with virus-infected(p < 0.05). Additionally, EPS extracts inhibited HSV-1 replication in the mice brain in a dose-dependent manner. Furthermore, the extracts decreased NF-κB protein and mRNA expression and the production of TNF-α in HSV-1-infected mice brain tissue. These effects were also dose-dependent. Our findings suggest that the EPS extract may be a potential candidate for developing an antiviral drug against HSV-1.
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http://dx.doi.org/10.1016/j.jviromet.2021.114246DOI Listing
July 2021

Serum metabolome and gut microbiome alterations in broiler chickens supplemented with lauric acid.

Poult Sci 2021 Sep 9;100(9):101315. Epub 2021 Jun 9.

College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, 311300, Hangzhou, China. Electronic address:

Antibiotic overuse in poultry husbandry poses a potential threat to meat safety and human health. Lauric acid (LA) is a primary medium-chain fatty acid (MCFA) with a strong antibacterial capacity. The goal of this study was to evaluate the beneficial effects of LA on the growth performance, immune responses, serum metabolism, and cecal microbiota of broiler chickens. One-day-old male Ross 308 broilers were randomly divided into 4 groups: CON, fed a basal diet; ANT, a basal diet supplemented with 75 mg/kg antibiotic; LA, a basal diet supplemented with 500 mg/kg LA; LA, a basal diet supplemented with 1000 mg/kg LA. The feeding period was 42 d. The results showed that LA significantly improved broiler growth and immune functions, as evidenced by increased body weight (BW) and average daily gain (ADG), enhanced intestinal mucosal barrier, upregulated immunoglobulins (IgA, IgM, and IgY), and downregulated inflammatory cytokines (IL-1β, IL-6, TNF-α, IL-4, and IL-10) (P < 0.05). HPLC/MS-based metabolome analysis revealed that the serum metabolites in the LA group differed from those of CON and ANT groups. LA markedly decreased the abundance of phosphatidylcholines (PCs), increased lysophosphatidylcholines (LysoPCs), and inhibited the sphingolipid metabolism pathway, indicating its capacity to modulate lipid metabolism. 16S rRNA sequencing indicated that LA significantly altered cecal microbiota composition by reducing Phascolarctobacterium, Christensenellaceae_R-7_group, and Bacteroides, and increasing Faecalibacterium and Ruminococcaceae_UCG-014 (P < 0.05). Furthermore, Spearman correlation analysis revealed that changes in metabolism and microbiota were highly correlated with the growth and immune indices; strong links were also found between lipid metabolism and microbial composition. Taken together, LA promotes broiler growth and immune functions by regulating lipid metabolism and gut microbiota. The above findings highlight the substantial potential of LA as a supplement in poultry diets and provide a new strategy to reduce antibiotic usage and improve food safety.
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http://dx.doi.org/10.1016/j.psj.2021.101315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318919PMC
September 2021

Metal-Nitrogen-Carbon Catalysts of Specifically Coordinated Configurations toward Typical Electrochemical Redox Reactions.

Adv Mater 2021 Aug 3;33(34):e2100997. Epub 2021 Jul 3.

State Key Lab of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.

Metal-nitrogen-carbon (M-N-C) material with specifically coordinated configurations is a promising alternative to costly Pt-based catalysts. In the past few years, great progress is made in the studies of M-N-C materials, including the structure modulation and local coordination environment identification via advanced synthetic strategies and characterization techniques, which boost the electrocatalytic performances and deepen the understanding of the underlying fundamentals. In this review, the most recent advances of M-N-C catalysts with specifically coordinated configurations of M-N (x = 1-6) are summarized as comprehensively as possible, with an emphasis on the synthetic strategy, characterization techniques, and applications in typical electrocatalytic reactions of the oxygen reduction reaction, oxygen evolution reaction, hydrogen evolution reaction, CO reduction reaction, etc., along with mechanistic exploration by experiments and theoretical calculations. Furthermore, the challenges and potential perspectives for the future development of M-N-C catalysts are discussed.
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http://dx.doi.org/10.1002/adma.202100997DOI Listing
August 2021

Systematic Evaluation of Clinical Efficacy and Platelet Function of Sofren Injection in the Treatment of Angina Pectoris.

Evid Based Complement Alternat Med 2021 26;2021:5591137. Epub 2021 Mar 26.

The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan 450000, China.

To systematically evaluate the efficacy and safety of sofren injection combined with conventional Western medicine in the treatment of angina pectoris. Randomized controlled trials (RCTs) on the treatment of angina pectoris with sofren injection combined with Western medicine were collected by searching PubMed, the Cochrane Library, Embase, Web of Science, CNKI, Wanfang Database, Weipu Database, and China Biomedical Literature Service System (CBM) by computer with the retrieval time from establishment of database to August 2020. After literature screening according to the predetermined inclusion and exclusion criteria, data of eligible studies were extracted, and then, a meta-analysis was conducted with the RevMan 5.3 software. The results of meta-analysis showed that the combination of sofren injection and Western medicine improved the platelet aggregation rate of patients (MD = -5.53, 95% CI (-6.42, -4.64), < 0.00001), PAI-1 (SMD = -2.29, 95% CI (-2.57, -2.01), < 0.00001), TXB2 (MD = -11.91, 95% CI (-14.50, -9.32), < 0.00001), duration of angina attack (MD = -2.01, 95% CI (-3.14, -0.87), =0.0005), ECG symptoms (RR = 1.29, 95% CI (1.20, 1.37), < 0.00001), whole blood viscosity (MD = -1.07, 95% CI (-1.66, -0.48), =0.0004), plasma viscosity (MD = -0.27, 95% CI (-0.35, -0.20), < 0.00001), fibrinogen (MD = -0.67, 95% CI (-0.84, -0.50), < 0.00001), whole blood high shear viscosity (MD = -1.04, 95% CI (-1.30, -0.79), < 0.00001), whole blood low shear viscosity (MD = -2.03, 95% CI (-2.53, -1.53), < 0.00001), CRP (MD = -1.96, 95% CI (-3.01, -0.91), =0.0003), IL-6 (MD = -2.79, 95% CI (-4.02, -1.55), < 0.00001), and TNF- (MD = -17.34, 95% CI (-25.86, -8.81), < 0.00001) and better than the Western medicine group, and there was no statistical significance in the incidence of adverse reactions between the two groups (=0.48). The clinical application of sofren injection combined with conventional Western medicine in the treatment of angina pectoris is clear and safe, so it is recommended for clinical application.
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http://dx.doi.org/10.1155/2021/5591137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019373PMC
March 2021

Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes.

J Cancer 2021 2;12(7):2050-2058. Epub 2021 Feb 2.

Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan, China.

Colorectal cancer (CRC) is one of the most common cancers with high mortality worldwide. Uncontrolled growth is an important hallmark of CRC. However, the mechanisms are poorly understood. Syntaxin 2 (STX2) expression was analyzed in 160 cases of paraffin-embedded CRC tissue by immunohistochemistry, in 10 cases of fresh CRC tissue by western blot, and in 2 public databases. Gain- and loss-of-function analyses were used to investigate the biological function of STX2 in CRC growth. Exosomes isolation, characterization, Co-immunoprecipitation (Co-IP), flow cytometry and fluorescence were conducted to study the molecular mechanism of STX2 in CRC growth. The expression of STX2 was obviously up-regulated in human CRC tissues. Overexpression of STX2 increased the growth of CRC cells and . Downregulation of STX2 repressed the growth of CRC. STX2 modulated exosomes secretion of CRC cells which might correlated with Rab8a expression. The secreted exosomes could be ingested by CRC cells, and ultimately promoted the growth of CRC by arresting the tumor cells at S phase. Our data provide evidence that STX2 promotes CRC growth by increasing exosomes secretion of CRC cells; And the modulation of STX2 in exosomes secretion correlates with Rab8a. Thus, our study identified a new mechanism of STX2 in CRC growth and may provide a possible strategy for CRC therapy.
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http://dx.doi.org/10.7150/jca.51494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974533PMC
February 2021

Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.

Int J Biol Macromol 2020 Dec 25;165(Pt B):2621-2629. Epub 2020 Oct 25.

School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address:

Many effects of Coprinus comatus are attributed to its polysaccharide components. Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs). The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated. At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared. The structural analysis exhibited that there were slight differences in the two prepared polysaccharides. However, both ICPs and CBPs could be utilized by these two strains. Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05). Therefore, this work demonstrates that ICPs could be an equivalent replacer for CBPs.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.163DOI Listing
December 2020

Silicate-Enhanced Heterogeneous Flow-Through Electro-Fenton System Using Iron Oxides under Nanoconfinement.

Environ Sci Technol 2021 03 24;55(6):4045-4053. Epub 2021 Feb 24.

The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Herein, a silicate-enhanced flow-through electro-Fenton system with a nanoconfined catalyst was rationally designed and demonstrated for the highly efficient, rapid, and selective degradation of antibiotic tetracycline. The key active component of this system is the FeO nanoparticle filled carbon nanotube (FeO-in-CNT) filter. Under an electric field, this composite filter enabled HO generation, which was converted to reactive oxygen species accompanied by the redox cycling of Fe/Fe. The presence of the silicate electrolyte significantly boosted the HO yield by preventing the O-O bond dissociation of the adsorbed OOH*. Compared with the surface coated FeO on the CNT (FeO-out-CNT) filter, the FeO-in-CNT filter demonstrated 1.65 times higher value toward the degradation of the antibiotic tetracycline. Electron paramagnetic resonance and radical quenching tests synergistically verified that the dominant radical species was the O or HO· in the confined FeO-in-CNT or unconfined FeO-out-CNT system, respectively. The flow-through configuration offered improved tetracycline degradation kinetics, which was 5.1 times higher (at flow rate of 1.5 mL min) than that of a conventional batch reactor. Liquid chromatography-mass spectrometry measurements and theoretical calculations suggested reduced toxicity of fragments of tetracycline formed. This study provides a novel strategy by integrating state-of-the-art material science, Fenton chemistry, and microfiltration technology for environmental remediation.
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http://dx.doi.org/10.1021/acs.est.1c00349DOI Listing
March 2021

Triptonide is a reversible non-hormonal male contraceptive agent in mice and non-human primates.

Nat Commun 2021 02 23;12(1):1253. Epub 2021 Feb 23.

Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, USA.

There are no non-hormonal male contraceptives currently on the market despite decades of efforts toward the development of "male pills". Here, we report that triptonide, a natural compound purified from the Chinese herb Tripterygium Wilfordii Hook F displays reversible male contraceptive effects in both mice and monkeys. Single daily oral doses of triptonide induces deformed sperm with minimal or no forward motility (close to 100% penetrance) and consequently male infertility in 3-4 and 5-6 weeks in mice and cynomolgus monkeys, respectively. Male fertility is regained in ~4-6 weeks after cessation of triptonide intake in both species. Either short- or long-term triptonide treatment causes no discernable systematic toxic side effects based on histological examination of vital organs in mice and hematological and serum biochemical analyses in monkeys. Triptonide appears to target junction plakoglobin and disrupts its interactions with SPEM1 during spermiogenesis. Our data further prove that targeting late spermiogenesis represents an effective strategy for developing non-hormonal male contraceptives.
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http://dx.doi.org/10.1038/s41467-021-21517-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902613PMC
February 2021

Protective Effects of Shenfuyixin Granule on HO-Induced Apoptosis in Neonatal Rat Cardiomyocytes.

Evid Based Complement Alternat Med 2021 29;2021:6654457. Epub 2021 Jan 29.

The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China.

Shenfuyixin granule (SFYXG, i.e., Xinshuaikang granule) is a prescription, commonly used in the clinical experience, which plays a significant role in the treatment of heart failure. The purpose of this present research was to investigate the protective effect of SFYXG, and the mechanism about anti-HO-induced oxidative stress and apoptosis in the neonatal rat cardiomyocytes. Myocardial cells, as is well known, were divided into 4 groups: normal, model, SFYXG, and coenzyme Q10 group, respectively. Cells viability was determined by MTT assay. Flow cytometry and AO/EB staining were implemented to test the apoptosis rate and intracellular reactive oxygen species (ROS) level. Mitochondrion membrane potential (MMP) was evaluated by JC-1 fluorescence probe method. The myocardial ultrastructure of mitochondrion was measured by electron microscope. The related mRNA expression levels of Bax, Bcl-2, Caspase-3, caspase-8, and caspase-9 were detected by real-time polymerase chain reaction (PCR). Also, the expression levels of Bax and Bcl-2 protein were detected by Western blot, and the expression levels of caspase-3, caspase-8, and caspase-9 protein were tested by caspase-Glo®3 Assay, caspase-Glo®8 Assay, and caspase-Glo®9 Assay, respectively. GAPDH was used as the internal reference gene/protein. The results revealed that SFYXG (0.5 mg/ml) raised the viability of myocardial cell, weakened the apoptosis rate and ROS level, corrected the mitochondrion membrane potential stability, and improved cell morphology and ultrastructure of myocardial mitochondrion. Furthermore, SFYXG upregulated the antiapoptosis gene of Bcl-2, but downregulated the proapoptosis genes of Bax, caspase-3, and caspase-9. In conclusion, SFYXG could appear to attenuate myocardial injury by its antioxidative and antiapoptosis effect.
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http://dx.doi.org/10.1155/2021/6654457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867454PMC
January 2021

Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts.

Front Immunol 2020 8;11:610934. Epub 2020 Dec 8.

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, The Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, China.

Grape seed is rich in vitamin E, flavonoids, and proanthocyanidins and has the potential to be used as an antibiotic substitute in broilers. We investigated the effects of grape seed proanthocyanidin extract (GSPE) on growth performance, immune responses, cecal microflora, and serum metabolism in early stage broilers. Data indicated that GSPE improved broiler growth performance by strengthening antioxidant capacity, enhancing immune responses, and increasing cecal short chain fatty acids. 16S rRNA sequencing indicated that GSPE changed the predominant cecal microflora and induced the metabolism of amino acids, lipids, and carbohydrates. An UPLC-Q-TOF/MS-based metabolomics analysis identified 23 serum metabolites (mainly related to lipid, amino acid, and alkaloid) were extremely changed by GSPE treatment. The correlations between the changes of cecal microflora and serum metabolites in birds fed with GSPE were analyzed. Hence, GSPE potentially provides active ingredients that may be used as antibiotic substitute and reduces environmental pollution by grape by-products.
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http://dx.doi.org/10.3389/fimmu.2020.610934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753974PMC
June 2021

Betulinic Acid Inhibits Endometriosis Through Suppression of Estrogen Receptor β Signaling Pathway.

Front Endocrinol (Lausanne) 2020 10;11:604648. Epub 2020 Dec 10.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Endometriosis is an inflammatory gynecological disorder characterized by endometrial tissue growth located outside of the uterine cavity in addition to chronic pelvic pain and infertility. In this study, we aim to develop a potential therapeutic treatment based on the pathogenesis and mechanism of Endometriosis. Our preliminary data showed that the expression of estrogen receptor β (ERβ) was significantly increased, while ERα was significantly decreased, in endometriotic cells compared to normal endometrial cells. Further investigation showed that betulinic acid (BA) treatment suppressed ERβ expression through epigenetic modification on the ERβ promoter, while had no effect on ERα expression. In addition, BA treatment suppresses ERβ target genes, including superoxide dismutase 2 (SOD2), nuclear respiratory factor-1 (NRF1), cyclooxygenase 2 (COX2), and matrix metalloproteinase-1 (MMP1), subsequently increasing oxidative stress, triggering mitochondrial dysfunction, decreasing elevated proinflammatory cytokines, and eventually suppressing endometriotic cell proliferation, mimicking the effect of ERβ knockdown. On the other hand, gain of ERβ by lentivirus infection in normal endometrial cells resulted in increased cell proliferation and proinflammatory cytokine release, while BA treatment diminished this effect through ERβ suppression with subsequent oxidative stress and apoptosis. Our results indicate that ERβ may be a major driving force for the development of endometriosis, while BA inhibits Endometriosis through specific suppression of the ERβ signaling pathway. This study provides a novel therapeutic strategy for endometriosis treatment through BA-mediated ERβ suppression.
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http://dx.doi.org/10.3389/fendo.2020.604648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759155PMC
June 2021

The Prebiotic-Like Effects of Polysaccharides on Gut Microbiota in Normal Mice and Those with Acute Alcoholic Liver Injury: A Comparative Study.

Evid Based Complement Alternat Med 2020 28;2020:2027570. Epub 2020 Nov 28.

School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.

This study aims to investigate the prebiotic-like effects of polysaccharides (CCP) on gut microbiota. Mice were divided into four groups: normal group (NG), alcohol group (AG), polysaccharides group (PG), and alcohol + polysaccharides group (APG). The gut microbiota structure of feces was analyzed by determining the V3-V4 region sequence in 16S rDNA. The results showed CCP could increase the diversity of gut microbiota. Compared with NG, PG had a significantly higher relative abundance of Firmicutes and Lactobacillaceae and a lower abundance of Rikenellaceae. These changes in gut microbiota result in positive effects on gut due to a series of prebiotic-like effects of CCP. At the same time, CCP could improve some adverse changes in gut microbiota caused by acute alcohol intake, such as the increased proportion of Firmicutes, Bacteroidetes, Muribaculaceae, and Lachnospiraceae and the decreased proportion of Rikenellaceae. In conclusion, the CCP has certain prebiotic effects not only on normal mice but also on mice with acute alcoholic liver injury.
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http://dx.doi.org/10.1155/2020/2027570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719511PMC
November 2020

Advances in the extraction, purification, structural-property relationships and bioactive molecular mechanism of Flammulina velutipes polysaccharides: A review.

Int J Biol Macromol 2021 Jan 2;167:528-538. Epub 2020 Dec 2.

School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address:

With the further research in recent years, Flammulina velutipes (F. velutipes), an edible mushroom, has great application value in many fields. As one of the main bioactive components in F. velutipes, polysaccharide has a series of functions such as anti-oxidation, immune regulation, anti-inflammation, liver protection, anti-tumor, anti-hyperlipidemia and so on. In this paper, the current progress in the extraction, purification, structural characteristics and bioactivities of F. velutipes polysaccharides (FVPs) were reviewed. Meanwhile, the structural-property relationships of FVPs were further discussed. In addition, based on in vitro and in vivo experiments, the possible mechanisms of bioactivities of FVPs were summarized. In order to understand FVPs more comprehensively, the application status and the future research work of FVPs were also introduced. Finally, we hope that our research can provide a reference for further research and development of FVPs.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.208DOI Listing
January 2021

Cu/S-Occupation Bifunctional Oxygen Catalysts for Advanced Rechargeable Zinc-Air Batteries.

ACS Appl Mater Interfaces 2020 Nov 12;12(47):52836-52844. Epub 2020 Nov 12.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Environmental Science and Engineering, Donghua University, 2999 Ren'min North Road, Shanghai 201620, P. R. China.

The design and synthesis of low-cost and highly efficient bifunctional catalysts is an inevitable path for rechargeable zinc-air batteries (rZABs). In this work, double-carbon co-supported Co-based oxide with the Cu and S substitutions are synthesized by a one-step hydrothermal method and formed a unique honeycomb structure. As expected, the (Cu, Co)[email protected] exhibits high oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) activity with low overpotential (0.86 V), high power density (215 mW cm), and long-term discharge stability (115 h). The (Cu, Co)[email protected] rZAB also shows a stronger charge-discharge durability with a very low voltage gap of merely 0.5 V than that of Pt/C+RuO. The high catalytic performances are attributed to these following reasons: (i) the porous morphology and hierarchical structure with plentiful "catalytic buffer", which accelerates the mass transfer; (ii) a high-speed electronic transmission network established by CN and carbon nanotube (CNT), enhancing the conductivity; (iii) the strong synergistic effect between (Cu, Co)[email protected] and CN, which improves the kinetics of ORR/OER; and (iv) the controllable occupation of Cu ions and S ions, which effectively regulates the CoO surface and increases the active site density. This work not only offers a promising ORR/OER electrode for rZAB but also provides a new pathway to understand the improvement mechanism for catalysts by the bi-ion substitutions.
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http://dx.doi.org/10.1021/acsami.0c16760DOI Listing
November 2020

Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila.

Curr Microbiol 2021 Jan 11;78(1):329-337. Epub 2020 Nov 11.

Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.

Phage therapy is an alternative approach to overcome the problem of multidrug-resistant bacteria. Here, a novel bacteriophage AhyVDH1, which infects Aeromonas hydrophila 4572, was isolated and its morphology, one-step growth curve, lytic activity, stability under various conditions, and genome were investigated. Transmission electron microscopy revealed that AhyVDH1 has an icosahedral head 49 nm in diameter and a contractile tail 127 nm in length, suggesting that it belongs to the family Myoviridae. AhyVDH1 showed strong adsorption to the surface of A. hydrophila 4572 (90% in 10 min). The latent period of AhyVDH1 was shown to be 50 min, and the burst size was 274 plaque-forming unit/infected cell. AhyVDH1 was stable at 30 °C for 1 h and lost infectivity after20 min of heating at 60 °C. Infectivity remained unaffected at pH 6-7 for 1 h, while the bacteriophage was inactivated at pH < 4 or > 11. AhyVDH1 has a 39,175-bp genome, with a 58% G + C content and 59 open reading frames. BLAST analysis indicated that the genome sequence of phage AhyVDH1 was related to that of Aeromonas phage Ahp2. Both time and MOI-dependent in vitro A. hydrophila growth inhibition were observed with AhyVDH1.Re-growth of the host bacteria appeared about 12 h after treatment, suggesting its potential therapeutic value in treating A. hydrophila infections, but phage cocktails should be developed.
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http://dx.doi.org/10.1007/s00284-020-02279-7DOI Listing
January 2021

Bimetallic Sulfide with Controllable Mg Substitution Anchored on CNTs as Hierarchical Bifunctional Catalyst toward Oxygen Catalytic Reactions for Rechargeable Zinc-Air Batteries.

ACS Appl Mater Interfaces 2020 Aug 5;12(33):37164-37172. Epub 2020 Aug 5.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Environmental Science and Engineering, Donghua University, 2999 Ren'min North Road, Shanghai 201620, P. R. China.

The exploitation of high-efficiency and cheap bifunctional cathode electrocatalyst is of significant importance to rechargeable zinc-air batteries. In this paper, a bimetallic sulfide coupled with a CNT ((Co, Mg)[email protected]) hybrid catalyst is developed via a proposed vulcanization process. The (Co, Mg)[email protected]) with controllable Mg substitution has a tailored crystal structure (amorphous and crystalline), which catalyzes the oxygen reduction/evolution reaction (ORR/OER). The active sites of [email protected] are activated by doping Mg ions, which accelerates the kinetics of the oxygen adsorption for ORR and oxygen desorption for OER. Meanwhile, the hybrid catalyst exhibits a unique hierarchal morphology and a "catalytic buffer", which further accelerate the mass transfer of catalytic processes. In addition, the outer wall of CNTs as substrate effectively avoid the agglomeration of (Co, Mg)S particles by reasonably providing adsorption sites. The inner and outer walls of CNTs form a high-speed conduction pathway, quickly transferring the electrons produced by oxygen catalytic reactions. As a result, the (Co, Mg)[email protected] exhibit an ORR performance comparable with commercial catalyst Pt/C-RuO and remarkable OER performance ( = 1.59 V). The high power density of 268 mW cm and long-term charge/discharge stability of the zinc-air battery proves the feasibility of (Co, Mg)[email protected] application in high-power devices.
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http://dx.doi.org/10.1021/acsami.0c09385DOI Listing
August 2020

Multiplex real-time SYBR Green I PCR assays for simultaneous detection of 15 common enteric pathogens in stool samples.

Mol Cell Probes 2020 10 18;53:101619. Epub 2020 Jun 18.

Chinese PLA Center for Disease Control and Prevention, Beijing, 100071, China. Electronic address:

Diarrheal diseases account for more than 50% of foodborne diseases worldwide, the majority of which occur in infants and young children. The traditional bacterial detection method is complex and time-consuming; therefore, it is necessary to establish a rapid and convenient detection method that can detect multiple pathogens simultaneously. In this study, we developed a set of five multiplex real-time SYBR Green I PCR assays to simultaneously detect 15 common enteric pathogens based on the Homo-Tag Assisted Non-Dimer system. These assays effectively reduced primer-dimer formation and improved the stability, uniformity, and amplification efficiency of multiplex PCR. The detection limit of the multiplex SYBR Green I PCR system was approximately 10-10 CFU/mL for stool specimens. Furthermore, we vitrified heat-unstable components on the cap of a reaction tube, showing that Taq DNA polymerase, dNTPs, primers, and SYBR Green I remained stable at 25 °C. In summary, we developed multiplex SYBR Green I PCR assays that can simultaneously detect 15 enteric pathogens. This method is comprehensive, rapid, inexpensive, accurate, and simple and displays high specificity.
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http://dx.doi.org/10.1016/j.mcp.2020.101619DOI Listing
October 2020

Comparative Analysis of Fungal Diversity in Rhizospheric Soil from Wild and Reintroduced Estimated via High-Throughput Sequencing.

Plants (Basel) 2020 May 8;9(5). Epub 2020 May 8.

Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, China.

is a critically endangered species and considered a "plant species with extremely small populations" (PSESP). It is an endemic species in southeastern Yunnan Province, China, with reproductive barriers. Rhizosphere fungi play a crucial role in plant growth and health. However, the composition, diversity, and function of fungal communities in wild and reintroduced rhizospheres remain unknown. In this study, Illumina sequencing of the internal transcribed spacer 2 (ITS2) region was used to analyze rhizospheric soil samples from wild and reintroduced . Thirteen phyla, 45 classes, 105 orders, 232 families, and 433 genera of fungi were detected. Basidiomycota and Ascomycota were dominant across all samples. The fungal community composition was similar between the wild and reintroduced rhizospheres, but the fungal taxa relative abundances differed. The fungal community richness was higher in the reintroduced rhizosphere than in the wild rhizosphere, but the diversity showed the opposite pattern. Soil nutrients and leaf litter significantly affected the fungal community composition and functional diversity. Here, the composition, structure, diversity, and ecological functions of the fungal communities in the rhizospheres of wild and reintroduced were elucidated for the first time, laying a foundation for future research and endangered species protection.
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http://dx.doi.org/10.3390/plants9050600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284792PMC
May 2020

Berberine Ameliorates Prenatal Dihydrotestosterone Exposure-Induced Autism-Like Behavior by Suppression of Androgen Receptor.

Front Cell Neurosci 2020 9;14:87. Epub 2020 Apr 9.

Department of Child Psychiatry, Kangning Hospital of Shenzhen, Shenzhen, China.

Many epidemiology studies have shown that maternal polycystic ovary syndrome (PCOS) results in a greater risk of autism spectrum disorders (ASD) development, although the detailed mechanism remains unclear. In this study, we aimed to investigate the potential mechanism and provide a possible treatment for PCOS-mediated ASD through three experiments: Experiment 1: real-time PCR and western blots were employed to measure gene expression in human neurons, and the luciferase reporter assay and chromatin immunoprecipitation (ChIP) was used to map the responsive elements on related gene promoters. Experiment 2: pregnant dams were prenatally exposed to dihydrotestosterone (DHT), androgen receptor (AR) knockdown (shAR) in the amygdala, or berberine (BBR), and the subsequent male offspring were used for autism-like behavior (ALB) assay followed by biomedical analysis, including gene expression, oxidative stress, and mitochondrial function. Experiment 3: the male offspring from prenatal DHT exposed dams were postnatally treated by either shAR or BBR, and the offspring were used for ALB assay followed by biomedical analysis. Our findings showed that DHT treatment suppresses the expression of estrogen receptor β (ERβ) and superoxide dismutase 2 (SOD2) through AR-mediated hypermethylation on the ERβ promoter, and BBR treatment suppresses AR expression through hypermethylation on the AR promoter. Prenatal DHT treatment induces ERβ suppression, oxidative stress and mitochondria dysfunction in the amygdala with subsequent ALB behavior in male offspring, and AR knockdown partly diminishes this effect. Furthermore, both prenatal and postnatal treatment of BBR partly restores prenatal DHT exposure-mediated ALB. In conclusion, DHT suppresses ERβ expression through the AR signaling pathway by hypermethylation on the ERβ promoter, and BBR restores this effect through AR suppression. Prenatal DHT exposure induces ALB in offspring through AR-mediated ERβ suppression, and both prenatal and postnatal treatment of BBR ameliorates this effect. We conclude that BBR ameliorates prenatal DHT exposure-induced ALB through AR suppression, this study may help elucidate the potential mechanism and identify a potential treatment through using BBR for PCOS-mediated ASD.
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http://dx.doi.org/10.3389/fncel.2020.00087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161090PMC
April 2020

Supported Atomically-Precise Gold Nanoclusters for Enhanced Flow-through Electro-Fenton.

Environ Sci Technol 2020 05 20;54(9):5913-5921. Epub 2020 Apr 20.

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.

Gold (Au) has been considered catalytically inert for decades, but recent reports have described the ability of Au nanoparticles to catalyze HO decomposition in the Haber-Weiss cycle. Herein, the design and demonstration of a flow-through electro-Fenton system based on an electrochemical carbon nanotube (CNT) filter functionalized with atomically precise Au nanoclusters (AuNCs) is described. The functionality of the device was then tested for its ability to catalyze antibiotic tetracycline degradation. In the functional filters, the Au core of AuNCs served as a high-performance Fenton catalyst; while the AuNCs ligand shells enabled CNT dispersion in aqueous solution for easy processing. The hybrid filter enabled HO production and catalyzed the subsequent HO decomposition to HO·. The catalytic function of AuNCs lies in their ability to undergo redox cycling of Au/Au under an electric field. The atomically precise AuNCs catalysts demonstrated superior catalytic activity to larger nanoparticles; while the flow-through design provided convection-enhanced mass transport, which yielded a superior performance compared to a conventional batch reactor. The adsorption behavior and decomposition pathway of HO on the filter surfaces were simulated by density functional theory calculations. The research outcomes provided atomic-level mechanistic insights into the Au-mediated Fenton reaction.
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http://dx.doi.org/10.1021/acs.est.0c00427DOI Listing
May 2020

Tongmai Yangxin intervening in myocardial remodeling after PCI for coronary heart disease: study protocol for a double-blind, randomized controlled trial.

Trials 2020 Mar 20;21(1):287. Epub 2020 Mar 20.

Center of Cardiology, The First Affiliated Hospital of Henan University of TCM, 19 Renmin Road, Jinshui District, Zhengzhou, 450100, Henan Province, China.

Background: Coronary heart disease (CHD) has become a common cardiovascular disease that seriously threatens the health of people. As reperfusion in the early phase and drug therapy, especially percutaneous coronary intervention (PCI), have become widely used in the clinic, the mortality of acute myocardial infarction in the short term has been reduced significantly. In addition, in 40%-56% of patients who experience myocardial infarction, cardiac dysfunction occurs and about 25%-33% develop heart failure.

Methods: This study was designed as a multicenter, double-blind, randomized, placebo-controlled, parallel-group, superiority trial. Participants were randomly assigned in a 1:1 ratio through a centrally controlled, computer-generated, simple randomization schedule. The primary outcome was left ventricular end-diastolic volume index = left ventricular end-diastolic volume/body surface area. The combined secondary outcomes include traditional Chinese medicine syndrome score, echocardiogram results, 6-minute walk test results, Seattle Angina Questionnaire score, cardiac magnetic resonance imaging results, biological indicators, dynamic electrocardiogram results, and experiment event rate. Assessments will be performed at baseline and at 4, 8, and 12 weeks after randomization.

Discussion: This trial will demonstrate that the addition of a Tongmai Yangxin pill (TMYX) to conventional treatment will intervene in the development of cardiac remodeling and cardiac dysfunction.

Trial Registration: This study was registered in the Chinese Clinical Trial Registry on 7 May 2019. The registration number is ChiCRT1900023023 (http://www.chictr.org.cn/showproj.aspx?proj=12370).
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http://dx.doi.org/10.1186/s13063-020-4208-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085139PMC
March 2020

MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.

Biomed Res Int 2020 9;2020:4983420. Epub 2020 Jan 9.

Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan, China.

Background: Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown.

Methods: Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Moreover, Spearman's correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC.

Results: The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR-384.

Conclusion: The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3'-UTR of PRKACB gene.
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http://dx.doi.org/10.1155/2020/4983420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973191PMC
September 2020

Insight into the Effect of TDMs on the Tribological Behaviors of the Ionic Liquid Composite Films.

Materials (Basel) 2020 Jan 2;13(1). Epub 2020 Jan 2.

College of Environmental Science and Engineering, Donghua University, 2999 Ren'min North Road, Shanghai 201620, China.

Ionic liquid (IL) combined with 2D materials has evoked considerable attention in the field of lubrication applications because of their speical structure and outstanding lubrication properties. However, the ambiguous effect of the 2D materials on the friction and anti-wear properties of the IL needs futher study. Here, we have obtained two families of IL composite films with additives of MoS 2 and graphene via a combined process of spin-coated and curing, and the distinction of the effects of two additives on the tribological performance of the IL films was studied. The friction tests showed that the friction coefficient and anti-wear life of the IL films were greatly enhanced after the addition of MoS 2 or graphene, which could be attributed to the improved load-carrying capacity and the second lubrication phase. Under a low addition content, graphene had more advantages to reduce the friction of the films, and MoS 2 was more beneficial to the tribological properties with the additional content increased. The films with low friction and good anti-wear properties may be valuable for the rational design of lubrication films for the practical engineering applications.
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http://dx.doi.org/10.3390/ma13010191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981414PMC
January 2020

Long Non-coding RNA LINC01787 Drives Breast Cancer Progression via Disrupting miR-125b Generation.

Front Oncol 2019 5;9:1140. Epub 2019 Nov 5.

Department of Pathology, Xinxiang Medical University, Xinxiang, China.

Breast cancer is still the most common and leading cause of cancer-related deaths in women worldwide. Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) have shown key regulator roles in various cancers. Previous reports have identified miR-125b as a critical tumor suppressor in breast cancer. However, the role of lncRNAs in breast cancer is far from well-characterized. In this study, we identified a novel lncRNA LINC01787, which specifically binds pre-miR-125b, inhibits the binding between DICER and pre-miR-125b, represses the processing of pre-miR-125b by DICER, and therefore induces pre-miR-125b accumulation and represses mature miR-125b generation. Functional assays showed that LINC01787 promotes breast cancer cell proliferation and migration and breast cancer xenograft growth , which is abolished by the mutation of pre-miR-125b binding sites on LINC01787 or overexpression of miR-125b. Furthermore, LINC01787 is up-regulated in breast cancer tissues and is associated with advanced stages and poor survival. The expression of LINC01787 is inversely associated with that of miR-125b in breast cancer tissues. In conclusion, our findings identified a novel up-regulated and oncogenic lncRNA LINC01787 in breast cancer, which binds pre-miR-125b and represses mature miR-125b generation. Our data suggests LINC01787 as a potential prognostic biomarker and therapeutic target for breast cancer.
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http://dx.doi.org/10.3389/fonc.2019.01140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848230PMC
November 2019

Cysteine-rich intestinal protein 1 silencing alleviates the migration and invasive capability enhancement induced by excessive zinc supplementation in colorectal cancer cells.

Am J Transl Res 2019 15;11(6):3578-3588. Epub 2019 Jun 15.

Department of Pathology, Xinxiang Medical University Xinxiang 453000, Henan Province, China.

Cysteine-rich intestinal protein 1 (CRIP1) is overexpressed in colorectal cancer (CRC) tissues and functions as an oncogene in regulating the migration and invasion of CRC cells. However, the underlying mechanism is unclear. CRIP1 has a role in zinc absorption and functions as an intracellular zinc transport protein. Here, we aimed to focus on the function of zinc and its underlying mechanism in CRC and determine whether CRIP1 promotes invasion and CRC metastasis through excessive zinc-induced epithelial-mesenchymal transition (EMT) by affecting the phosphorylated glycogen synthase kinase (GSK)-3beta. The results showed that ZnSO (Zn) supplementation in medium increased the labile intracellular zinc content. Furthermore, excessive Zn supplementation activated the GSK3/mTOR signaling pathway in both SW620 and LoVo cells, and excessive Zn supplementation promoted migration, invasion, and EMT of SW620 and LoVo cells. This migration promotion was alleviated by the specific mTOR inhibitor rapamycin, indicating that the GSK3/mTOR signaling pathway was involved in this process. CRIP1 silencing increased the labile intracellular zinc content and inhibited EMT and GSK3/mTOR signaling pathway. CRIP1 silencing alleviated the zinc supplementation effects on migration, invasion, EMT, and GSK3/mTOR signaling pathway. In conclusion, excessive Zn promotes migration and invasion capabilities of SW620 and LoVo cells through GSK3/mTOR signaling pathway-induced EMT.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614615PMC
June 2019
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