Publications by authors named "Yonghui Wang"

192 Publications

Efficacy and Safety Evaluation of Transurethral Resection of the Prostate versus Plasmakinetic Enucleation of the Prostate in the Treatment of Massive Benign Prostatic Hyperplasia.

Urol Int 2021 Feb 1:1-8. Epub 2021 Feb 1.

Department of Urology, Beijing TongRen Hospital, Capital Medical University, Beijing, China.

Background: Benign prostatic hyperplasia (BPH) is a common chronic progressive disease resulting in urinary obstruction in aging men. It comes to more and more patients with massive BPH with the aging of society and extension of life expectancy.

Objective: The aim of the study was to compare the clinical efficacy, safety, and complications between transurethral bipolar plasmakinetic enucleation of the prostate (PKEP) and transurethral resection of the prostate (TURP) in the treatment of massive BPH.

Design And Setting: Patients with BPH were divided into the PKEP group and the TURP group randomly. Intraoperative blood loss (BL), operation time (OT), resected tissue weight (RTW), gland resection ratio (GRR), postoperative indwelling ureter time (IUT), bladder fistula time (BFT) and hospital stay time (HST), preoperative and postoperative serum sodium concentration (SSC), hemoglobin concentration (HGB), prostate weight (PW), postvoid residual (PVR), maximum urinary flow rate (Qmax), international prostate symptom score (IPSS), quality of life (QOL), International Index of Erectile Function (IIEF), and other complications were analyzed and compared respectively.

Results: There was no statistical difference in preoperative IPSS, preoperative QOL score, preoperative PVR, preoperative Qmax, postoperative QOL score, postoperative PVR, postoperative Qmax, IPSS difference value (DV), Qmax DV, and PVR DV between the PKEP group and the TURP group (p > 0.05). OT, BL, IUT, BFT, HST, and postoperative IPSS in the PKEP group were significantly lower than that in the TURP group (p < 0.01). RTW and GRR in the PKEP group were significantly higher than that in the TURP group (p < 0.01). QOL DV in the PKEP group was higher than that in the TURP group (p < 0.05). There was statistical difference in SSC DV between the PKEP group and the TURP group (p < 0.05). There was significant statistical difference in postoperative PW, postoperative HGB, PW DV, and HGB DV between the PKEP group and the TURP group (p < 0.01). There was significant statistical difference in IPSS, QOL, PVR, and Qmax between postoperative value and preoperative value in both groups (p < 0.01). The incidence of transurethral resection syndrome, obturator nerve reflex, transient urinary incontinence, and retrograde ejaculation between the PKEP group and the TURP group has no statistical difference (p > 0.05). Capsule perforation, blood transfusion, secondary hemorrhage, bladder neck contracture, and urethral stricture in the PKEP group were lower than that in the TURP group (p < 0.05). Bladder spasm in the PKEP group was significantly lower than that in the TURP group (p < 0.01). There was no statistical difference in preoperative and postoperative IIEF-5, effective erectile frequency, telotism average tension, sustainable telotism average time, and sexual dissatisfaction between the PKEP group and the TURP group (p > 0.05).

Conclusions: PKEP and TURP have similar clinical efficacy in the treatment of massive BPH. PKEP has advantages in shorter OT, less BL, more GRR, and fewer complications, but the long-term therapeutic effect of PKEP needs further follow-up.
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http://dx.doi.org/10.1159/000511116DOI Listing
February 2021

The p53/miR-145a Axis Promotes Cellular Senescence and Inhibits Osteogenic Differentiation by Targeting Cbfb in Mesenchymal Stem Cells.

Front Endocrinol (Lausanne) 2020 11;11:609186. Epub 2021 Jan 11.

Department of Geriatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

The osteogenic differentiation capacity of senescent bone marrow mesenchymal stem cells (MSCs) is reduced. p53 not only regulates cellular senescence but also functions as a negative regulator in bone formation. However, the role of p53 in MSCs senescence and differentiation has not been extensively explored. In the present study, we investigated the molecular mechanism of p53 in MSCs senescence and osteogenic differentiation. We found that p53 was upregulated during cellular senescence and osteogenic differentiation of MSCs respectively induced by HO and BMP9. Similarly, the expression of p53-induced miR-145a was increased significantly. Furthermore, Overexpression of miR-145a in MSCs promoted cellular senescence and inhibited osteogenic differentiation. Then, we identified that p53-induced miR-145a inhibited osteogenic differentiation by targeting core binding factor beta (Cbfb), and the restoration of Cbfb expression rescued the inhibitory effects of miRNA-145a. In summary, our results indicate that p53/miR-145a axis exert its functions both in promoting senescence and inhibiting osteogenesis of MSCs, and the novel p53/miR-145a/Cbfb axis in osteogenic differentiation of MSCs may represent new targets in the treatment of osteoporosis.
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http://dx.doi.org/10.3389/fendo.2020.609186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829338PMC
January 2021

Hypermethylation of the PZP gene is associated with hepatocellular carcinoma cell proliferation, invasion and migration.

FEBS Open Bio 2021 Mar 21;11(3):826-832. Epub 2021 Feb 21.

Department of Medical Oncology, Lishui Municipal Central Hospital, Zhejiang Province, China.

Pregnancy zone protein (PZP), a member of the proteinase inhibitor I39 (-2-macroglobulin) family of proteins, is involved in the initiation and development of various tumors. The gene encoding PZP is hypermethylated and expressed at low levels in hepatocellular carcinoma (HCC) tissue and cells, but the function of PZP in HCC cells remains unclear. Here, we analyzed DNA methylation and mRNA expression of HCC in The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset. We identified 10 methylation-driven genes, of which PZP was significantly hypermethylated and poorly expressed in tumor tissue. We confirmed that PZP is highly methylated and poorly expressed in HCC cell lines via quantitative real-time PCR experiment and methylation-specific PCR. Furthermore, PZP markedly inhibited the proliferation, invasion and migration of HCC cells. These findings may provide a basis for exploring novel therapeutic targets for HCC.
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http://dx.doi.org/10.1002/2211-5463.13093DOI Listing
March 2021

Role of N-Cadherin in a Niche-Mimicking Microenvironment for Chondrogenesis of Mesenchymal Stem Cells .

ACS Biomater Sci Eng 2020 Jun 6;6(6):3491-3501. Epub 2020 May 6.

Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning 530021, China.

During the development of natural cartilage, mesenchymal condensation is the starting event of chondrogenesis, and mesenchymal stem cells (MSCs) experienced a microenvironment transition from primarily cell-cell interactions to a later stage, where cell-extracellular matrix (ECM) interactions dominate. Although micromass pellet culture has been developed to mimic mesenchymal condensation , the molecular mechanism remains elusive, and the transition from cell-cell to cell-ECM interactions has been poorly recapitulated. In this study, we first constructed MSC microspheres (MMs) and investigated their chondrogenic differentiation with functional blocking of N-cadherin. The results showed that early cartilage differentiation and cartilage-specific matrix deposition of MSCs in the group with the N-cadherin antibody were significantly postponed. Next, poly(l-lysine) treatment was transiently applied to promote the expression of N-cadherin gene, , and the treatment-promoted MSC chondrogenesis. Upon one-day culture in MMs with established cell-cell adhesions, collagen hydrogel-encapsulated MMs (CMMs) were constructed to simulate the cell-ECM interactions, and the collagen microenvironment compensated the inhibitory effects from N-cadherin blocking. Surprisingly, chondrogenic-differentiated cell migration, which has important implications in cartilage repair and integration, was found in the CMMs without N-cadherin blocking. In conclusion, our study demonstrated that N-cadherin plays the critical role in early mesenchymal condensation, and the collagen hydrogel provides a supportive microenvironment for late chondrogenic differentiation. Therefore, sequential presentations of cell-cell adhesion and cell-ECM interaction in an engineered microenvironment seem to be a promising strategy to facilitate MSC chondrogenic differentiation.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00149DOI Listing
June 2020

Efficacy and safety of prostatic artery embolization for benign prostatic hyperplasia: a systematic review and meta-analysis of randomized controlled trials.

Eur Radiol 2021 Jan 15. Epub 2021 Jan 15.

Department of Urology, Beijing Tongren Hospital, Capital Medical University, No1. Dongjiaomingxiang street, Dongcheng District, Beijing, 100730, China.

Objective: To investigate the efficacy and safety of prostatic artery embolization (PAE) vs. transurethral resection of the prostate (TURP) in patients affected by benign prostatic hyperplasia (BPH). We also reviewed mean changes from baseline in PAE at selected follow-up points.

Methods: PubMed, Web of Science, and Embase were searched up to May 1, 2020. Randomized controlled trials on PAE were collected according to specific inclusion and exclusion criteria. Meta-analyses were performed using RevMan 5.3, STATA 14, and GraphPad Prism 8. Pooled patient-reported scores and functional outcomes were calculated by using a fixed or random-effect model.

Results: Eleven articles met our selection criteria and ten independent patient series were included in the final analysis. Pooled estimates suggested no significant difference between TURP and PAE for patient-reported outcomes including International Prostate Symptom Score (2.32 (- 0.44 to 5.09)) and quality of life (0.18 (- 0.41 to 0.77)) at 12 months. PAE was less effective regarding improvements in most functional outcomes such as maximum flow rate, prostate volume, and prostate-specific antigen. Moreover, PAE may be associated with relatively fewer complications, lower cost, and shorter hospitalization. After the PAE procedure, the overall weighted mean differences for all outcomes except sexual health scores were significantly improved from baseline during follow-up to 24 months.

Conclusion: PAE is non-inferior to TURP with regard to improving patient-reported outcomes, though most functional parameters undergo more changes after TURP than after PAE. Moreover, PAE can significantly continue to relieve symptoms for 24 months without causing serious complications.

Key Points: • PAE is as effective as TURP in improving subjective symptom scores, with fewer complications and shorter hospitalization times. • PAE is inferior to TURP in the improvement of most functional outcomes. • Improvements due to PAE are durable during follow-up to 24 months.
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http://dx.doi.org/10.1007/s00330-020-07663-2DOI Listing
January 2021

The modulation of object-based attentional selection by facial expressions.

Q J Exp Psychol (Hove) 2021 Feb 8:1747021821991767. Epub 2021 Feb 8.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Human beings can show preferentially attentional bias to different facial expressions. However, it is unclear whether the modulation of selective attention by facial expressions is based on the face itself (object-based attention) or its location (space-based attention). This study aimed to test this problem by using faces with different emotional valences in the two-rectangle paradigm across three experiments. We found that there was no significant difference in space-based effect among the positive, neutral, and negative conditions. However, the object-based effect was larger for the negative condition than for the neutral and positive ones, because of its slower reaction times for the invalid different-object trials. The results indicated that the object-based attentional selection was modulated by facial expressions, and that faces expressing negative emotions hamper the disengagement of attention from the whole object (i.e., the face), instead of the certain location. Our study can further add support to the attentional prioritisation hypothesis over attentional spreading hypothesis.
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http://dx.doi.org/10.1177/1747021821991767DOI Listing
February 2021

Precise Molecular-Level Modification of Nafion with Bismuth Oxide Clusters for High-performance Proton-Exchange Membranes.

Angew Chem Int Ed Engl 2021 Mar 28;60(11):6076-6085. Epub 2021 Jan 28.

Key Lab of Polyoxometalate Science of Ministry of Education, Key Laboratory of Nanobiosensing and Nanobioanalys, Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P. R. China.

Fabricating proton exchange membranes (PEMs) with high ionic conductivity and ideal mechanical robustness through regulation of the membrane microstructures achieved by molecular-level hybridization remains essential but challenging for the further development of high-performance PEM fuel cells. In this work, by precisely hybridizing nano-scaled bismuth oxide clusters into Nafion, we have fabricated the high-performance hybrid membrane, Nafion-Bi -3 %, which showed a proton conductivity of 386 mS cm at 80 °C in aqueous solution with low methanol permeability, and conserved the ideal mechanical and chemical stabilities as PEMs. Moreover, molecular dynamics (MD) simulation was employed to clarify the structural properties and the assembly mechanisms of the hybrid membrane on the molecular level. The maximum current density and power density of Nafion-Bi -3 % for direct methanol fuel cells reached to 432.7 mA cm and 110.2 mW cm , respectively. This work provides new insights into the design of versatile functional polymer electrolyte membranes through polyoxometalate hybridization.
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http://dx.doi.org/10.1002/anie.202012079DOI Listing
March 2021

Discovery of tetrahydroquinolines and benzomorpholines as novel potent RORγt agonists.

Eur J Med Chem 2021 Feb 19;211:113013. Epub 2020 Nov 19.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 ZhanghengRoad, Pudong, Shanghai, 201203, China. Electronic address:

The retinoic acid receptor-related orphan receptor γt (RORγt) is an important nuclear receptor that regulates the differentiation of Th17 cells and production of interleukin 17(IL-17). RORγt agonists increase basal activity of RORγt and could provide a potential approach to cancer immunotherapy. Herein, hit compound 1 was identified as a weak RORγt agonist during in-house library screening. Changes in LHS core of 1 led to the identification of tetrahydroquinoline compound 6 as a partial RORγt agonist (max. act. = 39.3%). Detailed structure-activity relationship on substituent of the LHS core, amide linker and RHS arylsulfonyl moiety was explored and a novel series of tetrahydroquinolines and benzomorpholines was discovered as potent RORγt agonists. Tetrahydroquinoline compound 8g (EC = 8.9 ± 0.4 nM, max. act. = 104.5%) and benzomorpholine compound 9g (EC = 7.5 ± 0.6 nM, max. act. = 105.8%) were representative compounds with high RORγt agonistic activity in dual FRET assay, and they showed good activity in cell-based Gal4 reporter gene assay and Th17 cell differentiation assay (104.5% activation at 300 nM of 8g; 59.4% activation at 300 nM of 9g). The binding modes of 8g and 9g as well as the two RORγt inverse agonists accidentally discovered were also discussed.
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http://dx.doi.org/10.1016/j.ejmech.2020.113013DOI Listing
February 2021

Pregnancy and COVID-19: what anesthesiologists should know.

Minerva Anestesiol 2021 01 24;87(1):77-84. Epub 2020 Nov 24.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an Shaanxi, China -

Introduction: Anesthetic management of parturients with COVID-19 is a big challenge to anesthesiologists. Limited data are available about COVID-19 during pregnancy; however, information on illnesses associated with SARS and MERS might provide insights into COVID-19's effects during pregnancy.

Evidence Acquisition: Evidence from previous reports from SARS and MERS, and from COVID-19 cases were reviewed. Concepts from guidelines from the government and academic societies were collected as well.

Evidence Synthesis: The evidence was described and summarized.

Conclusions: Principles to minimize the risk of infection as well as optimize patients' safety during obstetric anesthesia were found to include careful evaluation, tight protection, and multi-discipline-based strategy. Though vertical transmission of COVID-19 still needs more definitive evidence, strict isolation is necessary for the newborn of COVID-19 mothers. Psychological support for the parturients is also an important issue during COVID-19 pandemic.
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http://dx.doi.org/10.23736/S0375-9393.20.14647-9DOI Listing
January 2021

Deformable liposomal codelivery of vorinostat and simvastatin promotes antitumor responses through remodeling tumor microenvironment.

Biomater Sci 2020 Dec;8(24):7166-7176

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd, Shanghai 201203, China.

The tumor microenvironment (TME) and its major component tumor-associated macrophages (TAM) play a pivotal role in the development of non-small cell lung cancer (NSCLC). An epigenetic drug-based combinatory therapeutic strategy was proposed and a deformable liposome system (D-Lipo) was developed for vorinostat and simvastatin codelivery for remodeling the TME. The application of deformable liposomes in systemic cancer drug delivery has been underexplored and its potential in cancer therapy is largely unknown. This work revealed that D-Lipo exhibited an enhanced intratumor infiltration ability. The proposed therapeutic strategy was characterized by a chemo-free regimen and TME remodeling function. D-Lipo efficiently inhibited the growth of the xenografted lung tumor. The anti-tumor mechanisms involved the repolarization of TAM from the M2 to M1 phenotype, anti-angiogenesis, and the consequent TME remodeling. As a result, the amounts of the anti-tumor M1 macrophages and the cytotoxic CD8+ T cells increased, while the amounts of the pro-tumor M2 macrophages and regulatory T cells (Tregs) reduced. It provides a promising avenue for epigenetic drug-based combination therapy for treating solid tumors.
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http://dx.doi.org/10.1039/d0bm01516dDOI Listing
December 2020

The Impact of Icariside II on Human Prostate Cancer Cell Proliferation, Mobility, and Autophagy via PI3K-AKT-mTOR Signaling Pathway.

Drug Des Devel Ther 2020 8;14:4169-4178. Epub 2020 Oct 8.

Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, People's Republic of China.

Introduction: The flavonol glycoside icariside II (ICA II) has been shown to exhibit a range of anti-tumor properties. Herein, we evaluated the impact of ICA II on human prostate cancer cell proliferation, motility, and autophagy, and we further evaluated the molecular mechanisms underlying these effects.

Methods: We treated DU145 human prostate cancer cells with a range of ICA II doses and then assessed their proliferation via CCK-8 assay, while flow cytometry was used to monitor apoptosis and cell cycle progression. We further utilized wound healing and transwell assays to probe the impact of ICA II on migration and invasion, and assessed autophagy via laser confocal fluorescence microscopy. Western blotting was further utilized to measure LC3-II/I, Beclin-1, P70S6K, PI3K, AKT, mTOR, phospho-AKT, phospho-mTOR, and phospho-P70S6K levels, with qRT-PCR being used to evaluate the expression of specific genes at the mRNA level.

Results: We found that ICA II was capable of mediating the dose- and time-dependent suppression of DU145 cell proliferation, causing these cells to enter a state of cell cycle arrest and apoptosis. We further determined that ICA II treatment was associated with significant impairment of prostate cancer cell migration and invasion, whereas autophagy was enhanced in treated cells relative to untreated controls.

Conclusion: Our results indicate that ICA II treatment is capable of suppressing human prostate tumor cell proliferation and migration while enhancing autophagy via modulating the PI3K-AKT-mTOR signaling pathway. As such, ICA II may be an ideal candidate drug for the treatment of prostate cancer.
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http://dx.doi.org/10.2147/DDDT.S268524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549881PMC
October 2020

Derivation and propagation of spermatogonial stem cells from human pluripotent cells.

Stem Cell Res Ther 2020 09 23;11(1):408. Epub 2020 Sep 23.

State Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai, 200127, China.

Objectives: This study is designed to generate and propagate human spermatogonial stem cells (SSCs) derived from human pluripotent stem cells (hPSCs).

Methods: hPSCs were differentiated into SSC-like cells (SSCLCs) by a three-step strategy. The biological characteristics of SSCLCs were detected by immunostaining with antibodies against SSC markers. The ability of self-renewal was measured by propagating for a long time and still maintaining SSCs morphological property. The differentiation potential of SSCLCs was determined by the generation of spermatocytes and haploid cells, which were identified by immunostaining and flow cytometry. The transcriptome analysis of SSCLCs was performed by RNA sequencing. The biological function of SSCLCs was assessed by xeno-transplantation into busulfan-treated mouse testes.

Results: SSCLCs were efficiently generated by a 3-step strategy. The SSCLCs displayed a grape-like morphology and expressed SSC markers. Moreover, SSCLCs could be propagated for approximately 4 months and still maintained their morphological properties. Furthermore, SSCLCs could differentiate into spermatocytes and haploid cells. In addition, SSCLCs displayed a similar gene expression pattern as human GPR125 spermatogonia derived from human testicular tissues. And more, SSCLCs could survive and home at the base membrane of seminiferous tubules.

Conclusion: SSCLCs were successfully derived from hPSCs and propagated for a long time. The SSCLCs resembled their counterpart human GPR125 spermatogonia, as evidenced by the grape-like morphology, transcriptome, homing, and functional characteristics. Therefore, hPSC-derived SSCLCs may provide a reliable cell source for studying human SSCs biological properties, disease modeling, and drug toxicity screening.
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http://dx.doi.org/10.1186/s13287-020-01896-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509941PMC
September 2020

Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe.

Anal Chem 2020 09 26;92(18):12613-12621. Epub 2020 Aug 26.

School of Pharmacy & Minhang Hospital, Fudan University, Shanghai 201301, China.

Senescence-associated diseases have severely diminished the quality of life and health of patients. However, a sensitive assay of these diseases remains limited due to a lack of straightforward methods. Considering that senescence-associated β-galactosidase (SA-β-Gal) is overexpressed in senescent cells, the detection of SA-β-Gal in senescent cells and tissues might be a feasible strategy for the early diagnosis of SA diseases. In this study, a β-galactosidase-activatable nanoprobe was developed for the imaging of senescent cells and vasculature in atherosclerotic mice via real-time monitoring of β-Gal. was fabricated by encapsulating a newly designed NIR ratiometric probe within a poly(lactic--glycolic) acid (PLGA) core. Nanoprobe showed good accumulation in arteries, thus successfully visualizing senescent cells and vasculature in atherosclerotic mice by tail vein injection. Our findings indicated that nanoprobe holds great potential for the early diagnosis and therapy of atherosclerosis and other aging-associated diseases.
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http://dx.doi.org/10.1021/acs.analchem.0c02670DOI Listing
September 2020

Decreased low-frequency brain effective connectivity in seafarers during voyages: a functional near-infrared spectroscopy study.

Physiol Meas 2020 10 6;41(9):095003. Epub 2020 Oct 6.

Department of Physical Medicine and Rehabilitation, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People's Republic of China. School of Mechanical and Aerospace Engineering, Nanyang Technological University, 639798, Singapore.

Objective: During voyages, seafarers experience psychological problems that act to decrease operational safety. Psychological problems in seafarers can lead to changes in functional brain networks. This study investigated the low-frequency brain effective connectivity (EC) in seafarers during voyages by using the coupling strength (CS) of functional near-infrared spectroscopy (fNIRS) imaging.

Approach: This study recruited 15 seafarers (seafarer group) working on a container ship and 15 healthy age-matched controls (control group). The EC was assessed using dynamic Bayesian inference (DBI) of the oxygenated hemoglobin concentration (delta HbO) as measured through a 14-channel fNIRS system. These channels covered the left and right prefrontal cortices (LPFC/RPFC), left and right motor cortices (LMC/RMC), and left and right occipital lobes (LOL/ROL).

Main Results: The EC levels of LPFC to RMC (F = 4.239, p = 0.049), LPFC to ROL (F = 5.385, p = 0.028), LOL to RPFC (F = 11.128, p = 0.002), ROL to RPFC (F = 10.714, p = 0.003) and LMC to ROL (F= 6.136, p = 0.02) were significantly lower in the seafarer group than in the control group. Correlation analysis revealed that the patient health questionnaire-9 (PHQ-9) scores were positively correlated with the systolic blood pressure (SBP) values, delta HbO values and EC levels, respectively. Meanwhile, the correlation analysis revealed that the SBP values significantly positively correlated with the CS values.

Significance: Decreased EC levels may be a marker of psychological subhealth in seafarers. The approach combines fNIRS and PHQ-9 scores, providing a quantitative method for the assessment of mental health problems and further help with better rehabilitation designs in seafarers during voyages.
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http://dx.doi.org/10.1088/1361-6579/abad13DOI Listing
October 2020

Discovery of carboxyl-containing biaryl ureas as potent RORγt inverse agonists.

Eur J Med Chem 2020 Sep 5;202:112536. Epub 2020 Jul 5.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China. Electronic address:

GSK805 (1) is a potent RORγt inverse agonist, but a drawback of 1 is its low solubility, leading to a limited absorption in high doses. We have explored detailed structure-activity relationship on the amide linker, biaryl and arylsulfonyl moieties of 1 trying to improve solubility while maintaining RORγt activity. As a result, a novel series of carboxyl-containing biaryl urea derivatives was discovered as potent RORγt inverse agonists with improved drug-like properties. Compound 3i showed potent RORγt inhibitory activity and subtype selectivity with an IC of 63.8 nM in RORγ FRET assay and 85 nM in cell-based RORγ-GAL4 promotor reporter assay. Reasonable inhibitory activity of 3i was also achieved in mouse Th17 cell differentiation assay (76% inhibition at 0.3 μM). Moreover, 3i had greatly improved aqueous solubility at pH 7.4 compared to 1, exhibited decent mouse PK profile and demonstrated some in vivo efficacy in an imiquimod-induced psoriasis mice model.
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http://dx.doi.org/10.1016/j.ejmech.2020.112536DOI Listing
September 2020

Surface Siloxane-Modified Silica Materials Combined with Metal-Organic Frameworks as Novel MALDI Matrixes for the Detection of Low-MW Compounds.

ACS Appl Mater Interfaces 2020 Aug 4;12(33):37793-37803. Epub 2020 Aug 4.

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.

Surface siloxane (3-aminopropyl triethoxysilane hydrolyzates)-modified silica materials were used as "initiators", which resulted in the release and desorption of intact molecules adsorbed on the surface of a matrix. A covalently cross-linked MIL-53(Al) material was used to enhance the ionization of analytes. Herein, we have provided an efficient matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) matrix strategy, which responded to both ion and laser irradiation with low background interference in the low-molecular-weight (MW) region. The matrixes MIL-53(Al), SBA-15@APTES, SiO@APTES, SBA-15@APTES@MOF, and SiO@APTES@MOF were synthetized and used for the analysis of a series of low-MW compounds to verify the effectiveness of the strategies. Compared to conventional matrixes, the surface-modified SBA-15@APTES@MOF and SiO@APTES@MOF had low background, high sensitivity, extensive applicability, good stability, and ultrahigh tolerance of salt concentrations. The detection limits of standard analytes were determined to range from 0.1 to 1 × 10 mg/mL for 16 amino acids as well as citric acid, reserpine, tetraethylammonium chloride, melamine, bisphenol A, and malachite green. These results could help in designing more efficient nanostructure-initiator materials and further promote the application of MALDI-TOF MS.
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http://dx.doi.org/10.1021/acsami.0c11404DOI Listing
August 2020

Adenosine A Receptor Antagonists for Cancer Immunotherapy.

J Med Chem 2020 11 30;63(21):12196-12212. Epub 2020 Jul 30.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.

Currently, the most promising therapeutic modality for cancer treatment is the blockade of immune checkpoint pathways, which has revolutionized cancer therapy in the past 15 years. Strategies targeting and modulating adenosine A receptor (AR), an emerging alternative immune checkpoint, have shown the potential to produce significant therapeutic effects. In this review, we describe the immunosuppressive activities of AR and AR in the tumor microenvironment (TME), followed by a summary and discussion of the structure-activity relationship (SAR) of the AR (and dual AR/AR) antagonists that have been experimentally confirmed to exert oncoimmunological effects. This review also provides an update on the compounds under clinical evaluation and insights into the ligand binding modes of the receptor.
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http://dx.doi.org/10.1021/acs.jmedchem.0c00237DOI Listing
November 2020

Intraoperative ultrasonography assistance for minimally invasive repair of the acute Achilles tendon rupture.

J Orthop Surg Res 2020 Jul 11;15(1):258. Epub 2020 Jul 11.

Department of Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250021, China.

Background: Minimally invasive repair is a better option for Achilles tendon rupture with low re-rupture and wound-related complications than conservative treatment or traditional open repair. The major problem is sural nerve injury. The purpose of this study was to evaluate the effect and advantage of the intraoperative ultrasonography assistance for minimally invasive repair of the acute Achilles tendon rupture.

Methods: A retrospective study was performed on 36 cases of acute Achilles tendon rupture treated with minimally invasive repair assisted with intraoperative ultrasonography from January 2015 to December 2017. The relationship of the sural nerve and small saphenous vein was confirmed on the preoperative MRI. The course of the small saphenous vein and the sural nerve was identified and marked by intraoperative ultrasonography. The ruptured Achilles tendon was repaired with minimally invasive Bunnell suture on the medial side of the small saphenous vein (SSV).

Results: All patients were followed up for at least 12 months. No sural nerve injury or other complications was found intraoperatively and postoperatively. All the patients returned to work and light sporting activities at a mean of 12.78 ± 1.40 weeks and 17.28 ± 2.34 weeks, respectively. The Mean American Orthopaedic Foot & Ankle Society (AOFAS) scores improved from 59.17 ± 5.31 preoperatively to 98.92 ± 1.63 at the time of 12 months follow-up. There was a statistically significant difference (P < 0.001). No patient complained of a negative effect on their life.

Conclusions: The minimally invasive repair assisted with intraoperative ultrasonography can yield good clinical outcomes, less surgical time, and less complications, especially sural nerve injury. It is an efficient, reliable, and safe method for acute Achilles tendon (AT) rupture.
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http://dx.doi.org/10.1186/s13018-020-01776-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353778PMC
July 2020

Metabolic modulation via mTOR pathway and anti-angiogenesis remodels tumor microenvironment using PD-L1-targeting codelivery.

Biomaterials 2020 10 12;255:120187. Epub 2020 Jun 12.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China; NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients, China; Zhongshan Branch, the Institute of Drug Research and Development, Chinese Academy of Sciences, Zhongshan, China. Electronic address:

Tumor microenvironment (TME) closely affects cancer progression by promoting cancer cell survival and proliferation, drug resistance, metastasis, and immunosuppression as well. Remodeling TME is a promising therapeutic strategy for anticancer. mTOR signaling is an essential regulator for cellular metabolism and tumor-associated macrophages (TAMs) repolarization. There is an integrated crosstalk among mTOR/metabolism/immunity. Angiogenesis can also regulate metabolism and immunity. Based on these, a potential therapeutic avenue was developed by targeting mTOR and angiogenesis to remodel tumor immune microenvironment (TIME). A dual-targeting delivery liposomal system was designed with dual-modification of PD-L1 nanobody and mannose ligands for co-delivering an mTOR inhibitor (rapamycin) and an anti-angiogenic drug (regorafenib). The liposomes were able to target both TAMs and cancer cells that overexpressed PD-L1 and mannose receptors. The liposomes efficiently reduced glycolysis, repolarized TAMs, inhibited angiogenesis, reprogrammed immune cells, and consequently arrested tumor growth.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120187DOI Listing
October 2020

Household air pollution from cooking and heating and its impacts on blood pressure in residents living in rural cave dwellings in Loess Plateau of China.

Environ Sci Pollut Res Int 2020 Oct 20;27(29):36677-36687. Epub 2020 Jun 20.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China.

Cave dwelling is an ancient and unique type of residence in the Loess Plateau of Northern China, where the economics are less-developed. The majority of the local dwellers rely on traditional solid fuels for cooking and heating, which can emit large amounts of particles into both indoor and outdoor environments. In this study, we measured the real-time household concentrations of PM and explored the association between personal daily PM exposure and blood pressure (BP). Cooking and heating activities with different energies made a great variation in the household PM air pollution, and residents using biomass had the highest personal PM exposure. Temperature and relative humidity are both significantly linear correlated with household PM air pollution. Besides, systolic blood pressure (SBP) was demonstrated to be positively associated with personal PM exposure: with each 10-μg/m incremental PM concentration when controlling all the other factors, SBP will increase by 0.36 mmHg (95% confident interval (CI) 0.05-0.0.77 mmHg). If solid fuels could be replaced with clean energies, personal PM exposure and SBP would reduce by more than 21% and 3.7%, respectively, calling for efficient intervention programs to mitigate household air pollution of cave dwellings and protect health of those residents.
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http://dx.doi.org/10.1007/s11356-020-09677-1DOI Listing
October 2020

Biotic stability mechanisms in Inner Mongolian grassland.

Proc Biol Sci 2020 06 3;287(1928):20200675. Epub 2020 Jun 3.

Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, People's Republic of China.

Biotic mechanisms associated with species diversity are expected to stabilize communities in theoretical and experimental studies but may be difficult to detect in natural communities exposed to large environmental variation. We investigated biotic stability mechanisms in a multi-site study across Inner Mongolian grassland characterized by large spatial variations in species richness and composition and temporal fluctuations in precipitation. We used a new additive-partitioning method to separate species synchrony and population dynamics within communities into different species-abundance groups. Community stability was independent of species richness but was regulated by species synchrony and population dynamics, especially of abundant species. Precipitation fluctuations synchronized population dynamics within communities, reducing their stability. Our results indicate generality of biotic stability mechanisms in natural ecosystems and suggest that for accurate predictions of community stability in changing environments uneven species composition should be considered by partitioning stabilizing mechanisms into different species-abundance groups.
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http://dx.doi.org/10.1098/rspb.2020.0675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341928PMC
June 2020

The Long Non-Coding RNA MALAT1 Enhances Ovarian Cancer Cell Stemness by Inhibiting YAP Translocation from Nucleus to Cytoplasm.

Med Sci Monit 2020 May 20;26:e922012. Epub 2020 May 20.

Department of Radiation Oncology, Lishui Municipal Central Hospital, Lishui, Zhejiang, China (mainland).

BACKGROUND The purpose of this work was to unearth the effects and underlying mechanism of long non-coding RNA (lncRNA) MALAT1 in ovarian cancer cell stemness. MATERIAL AND METHODS Western blot, quantitative polymerase chain reaction (qPCR) and sphere forming analysis were performed to evaluate the stem-like traits of cells and MALAT1-induced effects on ovarian cancer cell stemness. Cell viability was performed to evaluate MALAT1 role in the chemoresistance of ovarian cancer cells. RNA immunoprecipitation (RIP) and luciferase reporter analysis were constructed to investigate the underlying mechanisms. RESULTS Here, qPCR assay showed that MALAT1 level was remarkably higher in non-adherent spheres formed by adherent ovarian cancer cells, as well as cisplatin-resistant ovarian cancer cells. Additionally, MALAT1 knockdown reduced ovarian cancer cell stemness, characterized as the decrease of sphere forming ability, expression of stemness regulatory masters, and attenuation of cisplatin resistance. Moreover, MALAT1 interacted with yes-associated protein (YAP), inhibited its nuclear-cytoplasm translocation, promoted YAP protein stability and expression and thus increased its activity. Notably, rescuing expression of YAP attenuated the inhibition of MALAT1 knockdown on ovarian cancer cell stemness. CONCLUSIONS In conclusion, these results demonstrate a MALAT1/YAP axis responsible for ovarian cancer cell stemness.
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http://dx.doi.org/10.12659/MSM.922012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254939PMC
May 2020

Microarray profile of B cells from Graves' disease patients reveals biomarkers of proliferation.

Endocr Connect 2020 May 1;9(5):405-417. Epub 2020 May 1.

Department of Endocrinology, Fifth People's Hospital of Shanghai Fudan University, Shanghai, China.

B lymphocytes are the source of autoantibodies against the thyroid-stimulating hormone receptor (TSHR) in Graves' disease (GD). Characterization of autoimmune B-cell expression profiles might enable a better understanding of GD pathogenesis. To reveal this, the expression levels of long noncoding RNAs (lncRNAs) and mRNAs (genes) in purified B cells from patients with newly diagnosed GD and healthy individuals were compared using microarrays, which elucidated 604 differentially expressed lncRNAs (DE-lncRNAs) and 410 differentially expressed genes (DEGs). GO and pathway analyses revealed that the DEGs are mainly involved in immune response. A protein-protein interaction network presented experimentally validated interactions among the DEGs. Two independent algorithms were used to identify the DE-lncRNAs that regulate the DEGs. Functional annotation of the deregulated lncRNA-mRNA pairs identified 14 pairs with mRNAs involved in cell proliferation. The lncRNAs TCONS_00022357-XLOC_010919 and n335641 were predicted to regulate TCL1 family AKT coactivator A (TCL1A), and the lncRNA n337845 was predicted to regulate SH2 domain containing 1A (SH2D1A). TCL1A and SH2D1A are highly involved in B-cell proliferation. The differential expression of both genes was validated by qRT-PCR. In conclusion, lncRNA and mRNA expression profiles of B cells from patients with GD indicated that the lncRNA-mRNA pairs n335641-TCL1A, TCONS_00022357-XLOC_010919-TCL1A, and n337845-SH2D1A may participate in GD pathogenesis by modulating B-cell proliferation and survival. Therefore, the identified lncRNA and mRNA may represent novel biomarkers and therapeutic targets for GD.
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http://dx.doi.org/10.1530/EC-20-0045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7274554PMC
May 2020

Effect of icariside II and metformin on penile erectile function, glucose metabolism, reaction oxygen species, superoxide dismutase, and mitochondrial autophagy in type 2 diabetic rats with erectile dysfunction.

Transl Androl Urol 2020 Apr;9(2):355-366

Andrology Center, Peking University First Hospital, Beijing 100034, China.

Background: Icariside II (ICAII) is a flavonoid isolated from herb Epimedium that has been shown to improve erectile function in rats. However, ICAII's underlying mechanism remains unclear.

Methods: Type 2 diabetes mellitus erectile dysfunction (T2DMED) rats were induced by single intraperitoneal injection of 25 mg/kg streptozotocin (STZ) and fed a high-fat, high-sugar, and high-calorie diet for 8 weeks. In the control and T2DMED groups, rats were administered with normal saline; in the metformin (MET) group, rats were administered with MET at 0.2 g/kg/day; and in the ICAII + MET group, rats were administered with ICA II at 10 mg/kg/day and MET for 0.2 g/kg/day. The deposition of advanced glycation end products (AGEs), expression of receptor for AGEs (RAGEs), reactive oxygen species (ROS), and superoxide dismutase (SOD) activity, and corpus cavernosum smooth muscle cells (CCSMCs) mitochondrial autophagy were measured. We also evaluated the expression of LC3-II/I, Beclin-1, P70S6K, PI3K, AKT, mTOR, phospho-AKT, phospho-mTOR, and phospho-P70S6K.

Results: ICAII and MET can improve erectile function, and decrease the levels of fasting plasma glucose (FPG), hemoglobin A1c (HbA1c), and AGEs in rats with T2DMED. Furthermore, ICAII and MET can decrease excessive CCSMC mitochondrial autophagy and the level of RAGE and oxidant stress in rats with T2DMED. ICAII and MET may enhance signaling via the PI3K-AKT-mTOR pathway in order to reduce the excessive mitochondrial autophagy of CCSMCs.

Conclusions: ICAII may effectively improve penile erectile function via decreasing excessive CCSMCs mitochondrial autophagy, RAGE, and oxidant stress. Furthermore, ICAII may enhance signaling via the PI3K-AKT-mTOR pathway in order to reduce excessive CCSMC mitochondrial autophagy.
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http://dx.doi.org/10.21037/tau.2020.02.07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214985PMC
April 2020

Wholist-analytic cognitive styles modulate object-based attentional selection.

Q J Exp Psychol (Hove) 2020 Oct 24;73(10):1596-1604. Epub 2020 May 24.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Previous studies have shown that individual difference plays an important role in the object-based cueing effect observed in experiments on attentional selection. A wide range of studies have also used the theory of cognitive style to explain individual strategies in most cognitive processes. However, the characteristics of individuals that modulate object-based attentional selection are still unclear. To investigate the modulation of object-based attention by cognitive style, this study used a classic two-rectangle paradigm and compared space-based effects and object-based effects for individuals who had been preselected as wholists or analysts in terms of cognitive style. The results revealed that a space-based effect was obtained for both wholist and analytic individuals. However, an object-based effect was obtained only for analysts and not wholists, regardless of object orientation. The results further indicated that a wholist versus analytic cognitive style can modulate object-based attention by way of perceptual grouping. Our study provides the first evidence that object-based attention can indeed be influenced by individual characteristics and extends traditional model of cognitive style by indicating that wholist individuals tend to group two or more objects in a scene into one larger gestalt.
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http://dx.doi.org/10.1177/1747021820921345DOI Listing
October 2020

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma.

Mol Med Rep 2020 Jun 10;21(6):2395-2404. Epub 2020 Apr 10.

Department of Oncology, Lishui Municipal Central Hospital, Lishui, Zhejiang 323000, P.R. China.

Hepatocellular carcinoma (HCC) has become a major cause of cancer‑related mortality worldwide. Circular RNAs (circRNAs) are non‑coding RNAs that serve important roles in multiple cancers. However, the role of circRNAs in HCC remains largely unknown. In the present study, a circRNA microarray dataset of HCC samples, GSE97332, was downloaded from the gene expression omnibus database. Following data preprocessing, differentially expressed circRNAs between HCC tissues and normal tissues were determined using GEO2R. The circRNA‑miRNA interactions were predicted by the miRanda database. The miRTarbase database was used to search for target genes of the miRNAs. A circRNA‑miRNA‑mRNA network was constructed using Cytoscape based on the obtained circRNA, miRNA and mRNA. In this network, the upregulated circRNA hsa_circRNA_100084 was found to be involved in a competing endogenous relationship of hsa_circRNA_100084‑hsa‑miR‑23a‑5p‑ insulin‑like growth factor 2 (IGF2). The differential expression of hsa_circRNA_100084, hsa‑miR‑23a‑5p and IGF2 in HCC tissues and liver cancer cells was validated by reverse transcription‑quantitative PCR. Additionally, the interactions between hsa‑miR‑23a‑5p with hsa_circRNA_100084 and IGF2 were validated by dual‑luciferase reporter assays. Knocking down hsa_circRNA_100084 inhibited the proliferation, migration and invasion of liver cancer cells, while the simultaneous overexpression of IGF2 reversed the effects of hsa_circRNA_100084 knockdown. The results show that hsa_circRNA_100084 could promote the expression of IGF2 by acting as a sponge of hsa‑miR‑23a‑5p in liver cancer cells.
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http://dx.doi.org/10.3892/mmr.2020.11069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185283PMC
June 2020

Why Do you Often Stop your Impulsive Behavior at the Last Moment?

Psychon Bull Rev 2020 Jun;27(3):498-503

School of Psychology, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China.

Intentional inhibition, a critical ability of human cognitive control, is the capacity to internally withhold a to-be-performed action. In the voluntary decision-making process, the cognitive system continuously generates intentional inhibition to ensure appropriate actions. However, the temporal dynamic of intentional inhibition is unclear. This study investigated the role of temporal preparation in internally generated intentional inhibition using a novel task that involved a modified free-choice task and a foreperiod paradigm. The experiment included Go, No-Go and free-choice trials, and temporal preparation was manipulated by varying the duration and constancy of the interval between the warning stimulus and target stimulus. The results showed that a high degree of temporal preparation can strengthen the intentional decision to inhibit action on free choices. By demonstrating that intentional inhibition is enhanced with increased temporal preparation, the present study illustrates how the cognitive decision-making system controls the flexibility and strategy in human behavior.
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http://dx.doi.org/10.3758/s13423-020-01727-6DOI Listing
June 2020

Effect of superfine grinding on physicochemical and antioxidant properties of soybean residue powder.

Food Sci Nutr 2020 Feb 27;8(2):1208-1214. Epub 2020 Jan 27.

Food and Bioengineering College Xuchang University Xuchang Henan China.

Soybean residue is an underutilized, nutrient-rich by-product of soybean processing. To enhance its value, we subjected soybean residue to superfine grinding and measured the resulting physiochemical properties and antioxidant activities. We prepared powders with particle sizes of 115.35, 77.93, 39.38, 25.01, and 20.44 μm. As particle size decreased, the surface area (from 96.46 to 198.32 m/kg) and swelling capacity (from 2.05 to 10.62 ml/g) increased. Conversely, we observed decreases in the surface-number mean (from 23.07 to 11.20 μm), volume-surface mean (from 141.70 to 27.96 μm), angles of repose (from 48.30° to 31.46°), water holding capacity (from 7.86 to 4.39 g/g), and oil binding capacity (from 1.78 to 1.42 g/g). The water solubility index and antioxidant activity (reducing power and free radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid)) improved as particle size decreased. In conclusion, superfine grinding improved some properties of soybean residue. Additionally, our findings provide theoretical support for using superfine grinding in industrial food applications.
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http://dx.doi.org/10.1002/fsn3.1409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020326PMC
February 2020

Effect of Icariside II and Metformin on Penile Erectile Function, Histological Structure, Mitochondrial Autophagy, Glucose-Lipid Metabolism, Angiotensin II and Sex Hormone in Type 2 Diabetic Rats With Erectile Dysfunction.

Sex Med 2020 Jun 5;8(2):168-177. Epub 2020 Mar 5.

Beijing Lu He Hospital, Capital Medical University, Department of Urology, Beijing, China. Electronic address:

Introduction: Type 2 diabetes mellitus erectile dysfunction (T2DMED) is one of the common complications of type 2 diabetes mellitus (T2DM). Icariside II (ICA II), a flavonoid derived from Epimedium, has been shown to improve erectile function in T2DMED rats.

Aim: To investigate the effect of ICA II and metformin (MET) on penile erectile function, mitochondrial autophagy, glucose-lipid metabolism in rats with T2DMED.

Methods: In the control and T2DMED groups, rats were administered normal saline. In the MET group, rats were administered MET for 0.2 g/kg/day. In the ICA II+MET group, rats were administered ICA II for 10 mg/kg/day and MET for 0.2 g/kg/day.

Results: The number of mating rats, number of erectile rats, erection rate, erection frequency, intracorneal pressure, and intracorneal pressure/mean arterial pressure in the ICA II+MET group and control group were significantly higher than corresponding values in than T2DMED group. The absolute values of fasting plasma glucose, glycated haemoglobin in the ICA II+MET group, MET group, and control group were significantly lower than in the T2DMED group. The advanced glycation end product (AGE) values in the ICA II+MET group and the MET group were lower than in the T2DMED group. The receptors for the AGE values and angiotensin II values in the ICA II+MET group were lower than in the T2DMED and MET groups. The high-density lipoprotein values, testosterone values, nitric oxide synthase activity, and cyclic guanosine monophosphate content in the ICA II+MET and control groups were higher than in the T2DMED group. The low-density lipoprotein values, triglyceride values, estradiol values, and total cholesterol values in the ICA II+MET and control groups were lower than in the T2DMED group.

Conclusion: ICA II could increase erectile function and smooth muscle cell/collagen fibril proportions, decreased mitochondrial autophagy, and AGE concentrations and improve lipid metabolism, nitric oxide synthase activity, cyclic guanosine monophosphate content, testosterone, estradiol, and Ang II in rat with T2DMED. Zhang J, Li S, Zhang S, et al. Effect of Icariside II and Metformin on Penile Erectile Function, Histological Structure, Mitochondrial Autophagy, Glucose-Lipid Metabolism, Angiotensin II and Sex Hormone in Type 2 Diabetic Rats With Erectile Dysfunction. J Sex Med 2020;8:168-177.
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http://dx.doi.org/10.1016/j.esxm.2020.01.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261708PMC
June 2020

The influence of object similarity on real object-based attention: The disassociation of perceptual and semantic similarity.

Acta Psychol (Amst) 2020 Apr 3;205:103046. Epub 2020 Mar 3.

School of Psychology, Shaanxi Normal University, Xi'an 710062, China; Shaanxi Provincial Key Laboratory of Behavior & Cognitive Neuroscience, Xi'an 710062, China. Electronic address:

Perceptual and semantic similarity have an impact on object-based attention for the geometric objects. However, no previous studies have disassociated perceptual properties from the semantic ones of real objects that combine perceptual and semantic properties. It is unclear whether the perceptual and semantic similarity of real objects jointly or independently guides attentional deployment. The aim of the present study was to explore the influence of object similarity on object-based attention by using a variant of the two-rectangle paradigm and disassociating the perceptual and semantic similarity of real objects. The results indicated that when the semantic of objects was similar, the object-based effect was larger for the perceptually dissimilar condition than for the perceptually similar condition, because of slower response to invalid different-object location in a dissimilar condition. Moreover, when the perception of objects was similar, the object-based effect was larger for the semantically dissimilar condition than for the semantically similar condition, due to slower response to invalid different-object location in a dissimilar condition. These results suggest that perceptual and semantic similarity can independently guide attentional allocation to real objects and the similarity may constrain the object-based attention in a way of grouping. The current study implies that the attentional prioritization hypothesis is more flexible and effective to explain the real object-based attention and also has some implication to advertising design.
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http://dx.doi.org/10.1016/j.actpsy.2020.103046DOI Listing
April 2020