Publications by authors named "Yongbin Zhang"

119 Publications

Radiofrequency ablation reduces pain for knee osteoarthritis: a meta-analysis of randomized controlled trials.

Int J Surg 2021 Apr 18:105951. Epub 2021 Apr 18.

Department of Trauma center, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, 225000, China. Electronic address:

Background: Currently, there is poor evidence on the effect of radiofrequency ablation (RFA) on pain and knee function in patients with knee osteoarthritis (OA). We performed a meta-analysis on randomized controlled trials (RCTs) to determine the effectiveness and safety of RFA on pain and knee function in individuals with knee OA.

Methods: PubMed, EMBASE, Web of Science, Cochrane, Ovid and MEDLINE were systematically searched (up to March 20, 2021) to obtain literature focusing on the impact of RFA on knee OA, using the following keywords and their synonyms: "radiofrequency ablation", "neurotomy", "knee" and "osteoarthritis". Two authors independently evaluated the quality of the RCTs according to the Cochrane Handbook for Systematic Reviews of Interventions version. Pooled effects of this meta-analysis were calculated using STATA version 13.0.

Results: Eight RCTs were included for data extraction and meta-analysis. The present study indicated that there were significant differences between the two groups of patients who were treated or not treated with RFA on the pain intensity at 4 week (WMD= -0.504; 95% CI: -0.708 to -0.300; P < 0.001), 12 week (WMD= -0.280; 95% CI: -0.476 to -0.084; P = 0.005) and 24 week (WMD= -2.437; 95% CI: -4.742 to -0.132; P = 0.038). Furthermore, RFA was associated with improved outcome of the Western Ontario and McMaster Universities Arthritis index at 4 week (WMD= -3.189; 95% CI: -5.996 to -0.382, P = 0.026), 12 week (WMD= -3.706; 95% CI:-6.584 to -0.828, P =0.012) and 24 week (WMD= -2.437; 95% CI: -4.742 to -0.132). No serious adverse events were observed in all patients who received RFA (RD= -0.019; 95% CI: -0.053 to 0.016; P=0.294).

Conclusion: RFA showed better effectiveness in relieving pain and promoting function recovery in patients with knee OA. Considering the small sample size of the included studies, the results should be treated with caution.
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http://dx.doi.org/10.1016/j.ijsu.2021.105951DOI Listing
April 2021

Intestinal microbiota: A potential target for enhancing the antitumor efficacy and reducing the toxicity of immune checkpoint inhibitors.

Cancer Lett 2021 Jul 14;509:53-62. Epub 2021 Apr 14.

Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. Electronic address:

Accumulating evidence suggests that the intestinal microbiota is associated with the antitumor efficacy of immune checkpoint inhibitors (ICIs) and the occurrence of immune-related adverse events (irAEs) following ICI treatment. However, the mechanisms underlying these interactions remain unclear. Recent technological advances have allowed more extensive investigation into the interplay between the intestinal microbiota and the tumor immune microenvironment. Breakthroughs by two research groups revealed that Bifidobacterium enhanced the efficacy of ICIs via the stimulator of interferon genes (STING) and adenosine 2A receptor (AR) signaling pathways, highlighting the molecular mechanisms through which the intestinal microbiota modulates immunotherapy. In this review, we summarize recent findings related to the potential role and mechanisms of the gut microbiota in ICI therapy, available microbiota-targeting strategies, and ongoing clinical trials. Further we discuss the associated challenges that remain in this field of research. The current review aims to evaluate the potential of the intestinal microbiota in maximizing the antitumor efficacy of ICIs while minimizing their toxic effects and guiding the development of more specific treatment regimens.
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http://dx.doi.org/10.1016/j.canlet.2021.04.001DOI Listing
July 2021

Sensitivity analysis of acoustic eigenfrequencies by using a boundary element method.

J Acoust Soc Am 2021 Mar;149(3):2027

Institute of Sound and Vibration Research, Hefei University of Technology, Hefei, Anhui 230009, People's Republic of China.

This paper presents a boundary element-based scheme for the sensitivity analysis of acoustic eigenfrequencies of both interior and exterior acoustic systems. The nonlinear eigenvalue problem generated by the acoustic boundary element method is first reformulated into a generalized eigenvalue problem of reduced dimension through a contour integral approach. The sensitivity formulations for acoustic eigenfrequencies are then derived based on an adjoint method that uses both the right and left eigenvectors. The adaptive cross approximation in conjunction with the hierarchical matrices is used to reduce the solution burden of the boundary element systems. The Burton-Miller-type combined formulation is applied to shift the spurious eigenfrequencies and their sensitivities, and the strategies to identify the spurious results are suggested. Three numerical examples are used to verify the accuracy and applicability of the developed scheme.
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http://dx.doi.org/10.1121/10.0003622DOI Listing
March 2021

A Pyrene-Based Fluorescent Probe for Specific Detection of Cysteine and its Application in Living Cell.

J Fluoresc 2021 May 20;31(3):727-732. Epub 2021 Feb 20.

Scientific Instrument Center, Shanxi University, Taiyuan, 030006, China.

Cysteine (Cys) is an essential amino acid in organism, which is transformed from methionine in vivo and participates in protein synthesis and cell redox process. Therefore, the detection of Cys is of great significance. In this work, a novel fluorescent probe, (E)-3-(2-chloroquinolin-3-yl)-1-(pyren-3-yl) prop-2-en-1-one (PAQ) was designed and synthesized to specifically detect Cys. The response mechanism of the reaction between PAQ and Cys was due to the addition reaction of Cys to α,β-unsaturated ketone of PAQ. Interestingly, the addition of Cys induced significant fluorescence intensity enhancement at 462 nm. PAQ exhibited favorable sensing properties towards Cys such as the low limit of detection (0.27 μM) and fast response speed (2 min). In addition, PAQ displayed high selectivity and anti-interference ability toward Cys among various analytes. Notably, PAQ has been successfully used to image exogenous and endogenous Cys in HeLa cells.
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http://dx.doi.org/10.1007/s10895-021-02703-6DOI Listing
May 2021

PRRX1 promotes lymph node metastasis of gastric cancer by regulating epithelial-mesenchymal transition.

Medicine (Baltimore) 2021 Feb;100(6):e24674

Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou.

Background: Gastric cancer has multiple metastasis pathways, of which lymph node metastasis plays a dominant role. However, the specific mechanism of lymph node metastasis is still not unclear.

Methods: The bioinformatics technology was utilized to mine gene chip data related to gastric cancer and Epithelial-Mesenchymal Transition (EMT) in a high-throughput gene expression database (Gene Expression Omnibus, GEO), we screened out all genes that have differential expression levels in gastric cancer tissues and in adjacent normal gastric mucosa tissues. The corresponding function package of R language software were performed for gene annotation and cluster analysis, then enrichment analysis of genes with differential expression and protein interaction network diagram for correlation analysis were performed, we finally screened out the paired related homeobox 1 gene (PRRX1) related to EMT. Next, we collected 65 metastatic lymph node samples and 93 gastric cancer tissue samples. The expression levels of PRRX1 and EMT-related protein E-cadherin (E-ca) and vimentin (Vim) in gastric cancer tissues and metastatic lymph node tissues were determined by immunohistochemistry (IHC) staining of streptavidin-peroxidase (SP). The expression differences of PRRX1, E-ca and Vim in gastric cancer tissues and metastatic lymph node tissues as well as the correlation were analyzed by the experimental data, and the clinical significance was analyzed in combination with the clinicopathological data.

Results: The PRRX1 expression levels in gastric cancer tissues are significantly higher than that in adjacent normal gastric mucosa tissues. The positive expression rates of PRRX1, Vim and E-ca in gastric cancer and in metastatic lymph node tissues were significantly different. Comparing with that in gastric cancer, expression of PRRX1 and Vim was significantly down-regulated, and E-ca expression was significantly up-regulated in metastatic lymph nodes.

Conclusion: PRRX1 may promote lymph node metastasis of gastric cancer by regulating EMT, and then affect the prognosis of patients. PRRX1 may be used as a new biological indicator to predict or prevent lymph node metastasis in gastric cancer.
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http://dx.doi.org/10.1097/MD.0000000000024674DOI Listing
February 2021

Targeted graphene oxide for drug delivery as a therapeutic nanoplatform against Parkinson's disease.

Biomater Sci 2021 Mar;9(5):1705-1715

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

There has been an exponential increase in the rate of incidence of Parkinson's disease (PD) with aging in the global population. PD, the second most common neurodegenerative disorder, results from damaged dopamine neurons in the substantia nigra pars compacta (SNpc), along with the deposition of abnormal α-synuclein (α-Syn), and the progressive degeneration of neurons in striatal regions. Despite extensive investigations to understand the pathophysiology of PD to develop effective therapies to restrict its progression, there is currently no cure for PD. Puerarin (Pue) is a natural compound with remarkable anti-PD properties. However, its poor pharmacological properties, including poor water solubility, inadequate bioavailability, and incomplete penetration of the blood-brain barrier (BBB) have restricted its use for the treatment of PD. Nevertheless, advancements in nanotechnology have revealed the potential advantages of targeted drug delivery into the brain to treat PD. Here, we used Pue-loaded graphene oxide (GO) nanosheets, which have an excellent drug-loading ability, modifiable surface functional groups, and good biocompatibility. Then, Pue was transported across the BBB into the brain using lactoferrin (Lf) as the targeting ligand, which could bind to the vascular endothelial receptor on the BBB. In vivo and in vitro results indicated that this multifunctional brain targeted drug delivery system (Lf-GO-Pue) was an effective and safe therapy for PD.
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http://dx.doi.org/10.1039/d0bm01765eDOI Listing
March 2021

Bufarenogin induces intrinsic apoptosis via Bax and ANT cooperation.

Pharmacol Res Perspect 2021 02;9(1):e00694

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.

Toads have high medicinal value and have been used for medicinal purposes since the Tang Dynasty period (7th-10th Century AD). Bufarenogin, an active anti-tumor constituent of toad venom, shows anti-tumor activity. In this study, we investigated the inhibitory effects of bufarenogin on the growth and metastasis of colorectal cancer (CRC), particularly its effects on mediating intrinsic signaling pathways that initiate apoptosis. An orthotopic CRC model was established in nude mice via surgical orthotopic implantation to investigate tumor growth. Immunohistochemistry, immunofluorescence, and Western blotting assays were performed to evaluate protein expression. The in vitro results revealed the anti-proliferative effect of bufarenogin against CRC cells. Bufarenogin caused cell death via apoptosis, as revealed by Annexin V/7-amino-actinomycin D double staining, which was verified using a pan-caspase inhibitor. Bufarenogin induced B-cell lymphoma 2-associated X protein (Bax)-dependent intrinsic apoptosis, as demonstrated by mitochondrial translocation of Bax and cytoplasm release of HCT116 wild-type cells and cytochrome C (soluble pro-apoptotic factors). Additionally, we showed that adenine-nucleotide translocator interacted with Bax. Bufarenogin induced intrinsic apoptosis through the cooperation of Bax and adenine-nucleotide translocator and inhibited the metastasis and growth of orthotopical CRC cells.
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http://dx.doi.org/10.1002/prp2.694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796793PMC
February 2021

Tissue-specific deformable image registration using a spatial-contextual filter.

Comput Med Imaging Graph 2021 Mar 29;88:101849. Epub 2020 Dec 29.

Department of Radiation Physics, the University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX, 77030, USA. Electronic address:

Intensity-based deformable registration with spatial-invariant regularization generally fails when distinct motion exists across different types of tissues. The purpose of this work was to develop and validate a new regularization approach for deformable image registration that is tissue-specific and able to handle motion discontinuities. Our approach was built upon a Demons registration framework, and used the image context supplementing the original spatial constraint to regularize displacement vector fields in iterative image registration process. The new regularization was implemented as a spatial-contextual filter, which favors the motion vectors within the same tissue type but penalizes the motion vectors from different tissues. This approach was validated using five public lung cancer patients, each with 300 landmark pairs identified by a thoracic radiation oncologist. The mean and standard deviation of the landmark registration errors were 1.3 ± 0.8 mm, compared with those of 2.3 ± 2.9 mm using the original Demons algorithm. Particularly, for the case with the largest initial landmark displacement of 15 ± 9 mm, the modified Demons algorithm had a registration error of 1.3 ± 1.1 mm, while the original Demons algorithm had a registration error of 3.6 ± 5.9 mm. We also qualitatively evaluated the modified Demons algorithm using two difficult cases in our routine clinic: one lung case with large sliding motion and one head and neck case with large anatomical changes in air cavity. Visual evaluation on the deformed image created by the deformable image registration showed that the modified Demons algorithm achieved reasonable registration accuracy, but the original Demons algorithm produced distinct registration errors.
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http://dx.doi.org/10.1016/j.compmedimag.2020.101849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902440PMC
March 2021

High-Specific Fluorescence Probe for SO Detection and Bioimaging.

J Fluoresc 2021 Mar 4;31(2):363-371. Epub 2021 Jan 4.

Scientific Instrument Center, Shanxi University, Taiyuan, 030006, People's Republic of China.

It is well known that sulfite (SO) plays an indispensable role in various physiological processes. Abnormal levels of SO can trigger a wide variety of diseases involving respiratory, nervous and cardiovascular systems. Hence, it is necessary to find an efficient approach for detection of SO. In this study, a pyrene derivative, (E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl)phenyl acrylate (PPA), was designed and synthesized for monitoring SO. The probe possessed simple synthetic steps, excellent anti-interference ability and specific response to SO in the presence of other substances. The reaction between PPA and SO was ascribed to Michael addition and the detection mechanism was confirmed by HRMS spectra analysis and FTIR analysis. Additionally, PPA responded linearly to detect SO within the rang of 0-100 μM. The limit of detection was calculated as low as 0.17 μM in accordance with the recommendation of IUPAC (CDL =3sb/m). Notably, PPA was further applied in biological imaging in HepG2 cells, which provided a possibility to monitor SO in vivo.
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http://dx.doi.org/10.1007/s10895-020-02662-4DOI Listing
March 2021

Cockroach as a Vector of Blastocystis sp. is Risk for Golden Monkeys in Zoo.

Korean J Parasitol 2020 Oct 22;58(5):583-587. Epub 2020 Oct 22.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian City 271000, Shandong Province, China.

Blastocystis sp. is a kind of protozoa living in the intestinal tract of human and animals, which will cause intestinal diseases such as diarrhea, abdominal distension and vomiting. This paper was aimed to understand the infection of Blastocystis sp. In golden monkeys and the transmission path in North China. Thirty-seven feces samples from golden monkeys and 116 cockroach samples from Shijiazhuang Zoo were collected from July to October 2019 for PCR analysis of Blastocystis sp. Genetic diversity analysis was further conducted on the samples with positive PCR results. The results showed that the infection rate was 48.7% (18/37) in golden monkeys and 82.8% (96/116) in cockroaches, respectively. The genetic evolution analysis based on small subunit ribosomal RNA demonstrated that three subtypes (ST) of Blastocystis sp. including ST1, ST2, and ST3 existed in the intestinal tract of golden monkeys, while only ST2 was detected in the intestinal tract of cockroaches. This paper may provide supports for the quarantine and control of Blastocystis sp. for the zoo in Northern China.
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http://dx.doi.org/10.3347/kjp.2020.58.5.583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672233PMC
October 2020

The reduction performance of double bonds regulated by the competition of push-pull electron groups to realize the colorimetric and fluorescence recognition of hypochlorous acid.

Analyst 2020 Nov;145(22):7297-7302

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.

Based on its reducibility, the double bond can act as a reaction site for hypochlorous acid (HOCl), which had been demonstrated by a great deal of work. Nevertheless, the reactivity is influenced by the adjacent chemical environment. Therefore, in this work, we constructed a probe (QI) by methoxy-substituted quinoline conjugating dicyanoisoflurone, in which dicyano and pyridine N act as electron-withdrawing groups and the methoxy acts as an electron-donating group, to regulate their adjacent C[double bond, length as m-dash]C reactivity. The "push-pull" electron effect between the methoxy group and the pyridine N led to the C[double bond, length as m-dash]C bond being passivated. On the other hand, another C[double bond, length as m-dash]C bond was activated by the strong electron-pulling effect of the dicyano group. Thus, the previously weak intramolecular charge transfer became stronger after the dicyano adjacent to the C[double bond, length as m-dash]C was oxidized by HOCl, and showed a strong emission shifted from 570 to 520 nm along with a color change. The reaction mechanism was verified by mass spectrometry, NMR and theoretical calculation, and further bioimaging demonstrated the practical application of the probe.
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http://dx.doi.org/10.1039/d0an01551bDOI Listing
November 2020

A New Strategy: Distinguishable Multi-substance Detection, Multiple Pathway Tracing Based on a New Site Constructed by the Reaction Process and Its Tumor Targeting.

J Am Chem Soc 2020 10 13;142(43):18706-18714. Epub 2020 Oct 13.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, P. R. China.

In recent years, it has become a trend to employ organic molecular fluorescent probes with multireaction sites for the distinguishable detection and biological imaging of similar substances. However, the introduction of multireaction sites brought great challenges to organic synthesis, and at the same time, often destroyed the conjugated structure of the molecules, leading to an unsatisfactory fluorescence emission wavelength not conducive to practical application. As the eternal theme of life, metabolism goes on all the time. Metabolism is a series of ordered chemical reactions that occurs in the organism to maintain life. Chemical reactions in metabolism can be summarized as metabolic pathways. Simultaneous monitoring of different metabolic pathways of the same substance poses a lofty challenge to the probe. Here, we developed a new strategy: to construct new sites through the preliminary reactions between probes and some targets, which can be used to further distinguish among targets or detect their metabolites, so as to realize the simultaneous visualization tracer of multiple metabolic pathways. By intravenous injection, it revealed that the probe containing benzopyrylium ion can target tumors efficiently, and thiols are highly expressed in tumors compared to other tissues (heart, lung, kidney, liver, etc.). The consumption of thiols by the probe could not prevent tumor growth, suggesting that the tumor cure was not correlated with thiol concentration. The construction of new sites in the reaction process is a novel idea in the pursuit of multiple reaction sites, which will provide more effective tools for solving practical problems.
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http://dx.doi.org/10.1021/jacs.0c10210DOI Listing
October 2020

Relationship between PRRX1, circulating tumor cells, and clinicopathological parameter in patients with gastric cancer.

J BUON 2020 May-Jun;25(3):1455-1462

Department of Surgical Oncology, Gansu Province People's Hospital, Lanzhou 730000, China.

Purpose: Paired related homoeobox 1 (PRRX1) has been identified as a new epithelial-mesenchymal transition (EMT) inducer in gastric cancer and that PRRX1 upregulation is closely correlated with gastric cancer metastasis. In addition, circulating tumor cells (CTCs) play an important role in the process of gastric cancer's distant metastasis. Our study aimed to correlate Prrx1, CTCs and the clinicopathological parameters in primary gastric cancer patients.

Methods: Expressions of PRRX1 in a sample of 95 gastric carcinoma and adjacent nontumorous tissues were detected by immunohistochemistry. Then the integrated subtraction enrichment and immunostaining fluorescence in situ hybridization (SET-imFISH) platform were applied to detect and characterize CTCs in patients with gastric cancer. Finally, their correlations with clinicopathological parameters could be analyzed.

Results: The positive rate of PRRX1in gastric cancer was 56.84% and the rate was 36.84% in adjacent normal gastric mucosa, which was confirmed to be statistically significant. In the meantime, both the expression of PRRX1 and the positive rate of CTCs did not significantly correlate with age, gender or histologic type (p>0.05) but significantly related to tumor size, grade of differentiation, lymph node invasion, vascular invasion, metastasis status, depth of tumor invasion, lymph node metastasis and TNM stage (p<0.05). Besides, there was a close relationship between the PRRX1 of gastric cancer and the CTCs of peripheral blood specimens of cancer patients with the correlation coefficient 0.322.

Conclusion: Gastric cancer tissues showed that the level of PRRX1 expression was higher compared to the adjacent normal gastric mucosa. Both the expression of PRRX1 and the positive rate of CTCs significantly correlated with clinicopathological parameters. In addition, there was a positive correlation relationship between the PRRX1 of gastric cancer and the CTCs of peripheral blood specimens of cancer patients. These findings demonstrate that higher-level expression of PRRX1 in gastric cancer tissues increased the amount of CTCs in peripheral blood and facilitated the invasion and metastasis in patients with gastric cancer. Meanwhile, it gave some clues to clinical treatment. CTCs may contribute to promotion in diagnosis, therapy monitoring and prognosis of gastric cancer.
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February 2021

Carbazole-conjugated-coumarin by enone realizing ratiometric and colorimetric detection of hypochlorite ions and its application in plants and animals.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Dec 11;243:118813. Epub 2020 Aug 11.

Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China. Electronic address:

Detection of hypochlorite ions (ClO) in the organisms is of great significance for finding effective treatments for inflammations and diseases. Recently, fluorescent probes have aroused wide public concern as one of the effective tools for detecting molecules and ions. Nevertheless, due to low sensitivity and poor biocompatibility, the effect of fluorescent probes for biological imaging is still not ideal. For this, we developed a novel ratiometric fluorescent probe, 7-(diethylamino)-3-((E)-3-(9-ethyl-9H-carbazol-3-yl)acryloyl)-2H-chromen-2-one (DCC), which could be used for colorimetric detection of ClO. Study showed that, the detection mechanism of DCC is that probe can be rapidly oxidized to an enoic acid by ClO, resulting in a series of changes in spectral properties. This mechanism was confirmed experimentally and verified by theoretical calculations. It is worth mentioning that DCC has not only been successfully applied to the detection of exogenous and endogenous OCl in living cells, but also used for the detection of ClO in zebrafish, and Arabidopsis.
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http://dx.doi.org/10.1016/j.saa.2020.118813DOI Listing
December 2020

Combination of four clinical indicators predicts the severe/critical symptom of patients infected COVID-19.

J Clin Virol 2020 07 13;128:104431. Epub 2020 May 13.

Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China. Electronic address:

Background: Despite the death rate of COVID-19 is less than 3%, the fatality rate of severe/critical cases is high, according to World Health Organization (WHO). Thus, screening the severe/critical cases before symptom occurs effectively saves medical resources.

Methods And Materials: In this study, all 336 cases of patients infected COVID-19 in Shanghai to March 12th, were retrospectively enrolled, and divided in to training and test datasets. In addition, 220 clinical and laboratory observations/records were also collected. Clinical indicators were associated with severe/critical symptoms were identified and a model for severe/critical symptom prediction was developed.

Results: Totally, 36 clinical indicators significantly associated with severe/critical symptom were identified. The clinical indicators are mainly thyroxine, immune related cells and products. Support Vector Machine (SVM) and optimized combination of age, GSH, CD3 ratio and total protein has a good performance in discriminating the mild and severe/critical cases. The area under receiving operating curve (AUROC) reached 0.9996 and 0.9757 in the training and testing dataset, respectively. When the using cut-off value as 0.0667, the recall rate was 93.33 % and 100 % in the training and testing datasets, separately. Cox multivariate regression and survival analyses revealed that the model significantly discriminated the severe/critical cases and used the information of the selected clinical indicators.

Conclusion: The model was robust and effective in predicting the severe/critical COVID cases.
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http://dx.doi.org/10.1016/j.jcv.2020.104431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219384PMC
July 2020

Alkali-metal organomagnesiate complexes as catalysts for highly chemoselective crossed-Tishchenko reactions.

Dalton Trans 2020 May;49(19):6418-6424

Scientific Instrument Center, Shanxi University, Taiyuan, 030006, P. R. China. and Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, P. R. China.

Five new heterobimetallic magnesiates bearing bidentate dianionic pyrrolyl ligands have been synthesized through co-complexation with alkali-metal reagents and di-n-butylmagnesium. Single-crystal X-ray structural analysis of these complexes revealed a variety of intriguing bonding modes. These alkali-metal (Li, Na, and K) organomagnesiates were utilized as catalysts for cross-coupling Tishchenko reactions with two different aldehydes. The sodium alkyl magnesiate complex {nBuMg[2-(Me3CNCH2)C4H3N]Na(Et2O)}∞ (3) was identified as a competent catalyst, as it exhibited higher catalytic activities and chemoselectivity under mild conditions.
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http://dx.doi.org/10.1039/d0dt01114bDOI Listing
May 2020

Dual-site functionalized NIR fluorescent material for a discriminative concentration-dependent response to SO in cells and mice.

J Mater Chem B 2019 03 18;7(11):1945-1950. Epub 2019 Feb 18.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy, Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.

Sulfur dioxide (SO), as an important anti-oxidant and gaseous signaling molecule, plays fundamental roles in the regulation of intracellular signaling and cell death cellular bioenergetics. The accurate determination of the SO levels in an organism is a critical step in establishing its biological functions. Therefore, there is a high demand for developing efficient analytical methods for monitoring the wide range of concentration changes of SO in living systems. Herein, for the first time, we have successfully constructed a dual-site functionalized NIR fluorescent material (NIR-SP), which is capable of monitoring low concentrations of SO with red fluorescence responses and high concentrations of SO with blue fluorescence enhancements. Further studies absolutely confirmed that the discriminative response was due to the fact that the reactivity of the unsaturated C[double bond, length as m-dash]C double bond (site I) in NIR-SP was superior to that of the aldehyde group (site II). Furthermore, detection in a concentration-dependent manner was demonstrated by fluorescent imaging in cells and mice. We envision that this strategy would lead to insights into SO concentration-dependent biological effects and cell signals.
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http://dx.doi.org/10.1039/c8tb03253jDOI Listing
March 2019

Cytotoxicity and genotoxicity of cadmium oxide nanoparticles evaluated using in vitro assays.

Mutat Res 2020 Feb - Mar;850-851:503149. Epub 2020 Jan 28.

Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA. Electronic address:

Cadmium oxide nanoparticles (CdO NPs) are among some of the most studied and industrially used metal oxide NPs. They have been widely used for industrial application, such as paint pigments and electronic devices, and medical therapeutics. With increasing use of CdO NPs and concerns for their potential adverse effects on the environment and public health, evaluation of the cytotoxicity and genotoxicity of CdO NPs becomes very important. To date, there is a limited understanding of the potential hazard brought by CdO NPs and a lack of information and research, particularly on the genotoxicity assessment of these NPs. In this study, 10 nm CdO core-PEG stabilized NPs were synthesized, characterized and used for evaluation of CdO NPs' cytotoxicity and genotoxicity. Release of cadmium ions (Cd) from the CdO NPs in cell culture medium, cellular uptake of the NPs, and the endotoxin content of the particles were measured prior to the toxicity assays. Cytotoxicity was evaluated using the MTS assay, ATP content detection assay, and LDH assay. Genotoxicity was assessed using the Ames test, Comet assay, micronucleus assay, and mouse lymphoma assay. The cytotoxicity of cadmium chloride (CdCl) was also evaluated along with that of the CdO NPs. The results showed that endotoxin levels within the CdO NPs were below the limit of detection. CdO NPs induced concentration-dependent cytotoxicity in TK6 and HepG2 cells with the MTS, ATP and LDH assays. Although the genotoxicity of CdO NPs was negative in the Ames test, positive results were obtained with the micronucleus, Comet, and mouse lymphoma assays. The negative response of CdO NPs with the Ames test may be the result of unsuitability of the assay for measuring NPs, while the positive responses from other genotoxicity assays suggest that CdO NPs can induce chromosomal damage, single or double strand breaks in DNA, and mutations. The toxicity of the CdO NPs results from the NPs themselves and not from the released Cd, because the ions released from the NPs were minimal. These results demonstrate that CdO NPs are cytotoxic and genotoxic and provide new insights into risk assessment of CdO NPs for human exposure and environmental protection.
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http://dx.doi.org/10.1016/j.mrgentox.2020.503149DOI Listing
April 2020

Mitochondria-targeted reversible ratiometric fluorescent probe for monitoring SO/HCHO in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 12;234:118253. Epub 2020 Mar 12.

Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. Electronic address:

Sulfur dioxide (SO) maintains a certain steady state balance in the body, high concentration SO will be harmful to human health. Seeking a suitable detection method to monitor sulfur dioxide in real time becomes an urgent requirement owing to the transient nature of sulfur dioxide in organisms. Here, a novel NIR ratiometric fluorescent probe for detection of SO was developed based on a conjugation of coumarin and indol salt with excellent water solubility. The probe Mito-CI displayed highly sensitive (69 nM), fast response time (30 s), large Stokes shift (174 nm) and the NIR fluorescence emission wavelength (655 nm). In the reversibility process of the SO-probe Mito-CI system induced by HCHO in vitro was also detected. Besides, cell imaging showed that Mito-CI possesses mitochondria-targeted ability. Particularly, Mito-CI was proved to reversibly detect SO/HCHO in living cells.
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http://dx.doi.org/10.1016/j.saa.2020.118253DOI Listing
June 2020

Measurement-based study on characterizing symmetric and asymmetric respiratory motion interplay effect on target dose distribution in the proton pencil beam scanning.

J Appl Clin Med Phys 2020 Apr 14;21(4):59-67. Epub 2020 Mar 14.

Department of Radiation Oncology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Pencil beam scanning proton therapy makes possible intensity modulation, resulting in improved target dose conformity and organ-at-risk (OAR) dose sparing. This benefit, however, results in increased sensitivity to certain clinical and beam delivery parameters, such as respiratory motion. These effects can cause plan degeneration, which could lead to decreased tumor dose or increased OAR dose. This study evaluated the measurements of proton pencil beam scanning delivery made with a 2D ion chamber array in solid water on a 1D motion platform, where respiratory motion was simulated using sine and cosine waves representing sinusoidal symmetric and realistic asymmetric breathing motions, respectively. Motion amplitudes were 0.5 cm and 1 cm corresponding to 1 cm and 2 cm of maximum respiratory excursions, respectively, with 5 sec fixed breathing cycle. The treatment plans were created to mimic spherical targets of 3 cm or 10 cm diameter located at 5 cm or 1 cm depth in solid water phantom. A reference RBE dose of 200 cGy per fraction was delivered in 1, 5, 10, and 15 fractions for each dataset. We evaluated dose conformity and uniformity at the center plane of targets by using the Conformation Number and the Homogeneity Index, respectively. Results indicated that dose conformity as well as homogeneity was more affected by motion for smaller targets. Dose conformity was better achieved for symmetric breathing patterns than asymmetric breathing patterns regardless of the number of fractions. The presence of a range shifter with shallow targets reduced the motion effect by improving dose homogeneity. While motion effects are known to be averaged out over the course of multifractional treatments, this might not be true for proton pencil beam scanning under asymmetrical breathing pattern.
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http://dx.doi.org/10.1002/acm2.12846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170285PMC
April 2020

Extract of Danggui-Shaoyao-San ameliorates cognition deficits by regulating DHA metabolism in APP/PS1 mice.

J Ethnopharmacol 2020 May 18;253:112673. Epub 2020 Feb 18.

Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun District, Guangzhou, 510405, China. Electronic address:

Ethnopharmacological Relevance: The traditional Chinese medicine formula Danggui-Shaoyao-San (DSS) has been reported to show therapeutic effect on alleviating the symptoms of Alzheimer's disease (AD).

Aim Of The Study: The present study aims to investigate the relation between DSS treatment of AD and DHA metabolism and evaluates its neuroprotective effect on cognitive in APP/PS1 mice.

Material And Methods: DSS (1.6, 3.2, 6.4 g/kg/day) or Aricept (3 mg/kg/day) was orally administered (i.g.) to APP/PS1 mice, and saline was orally administered to Wild-type (WT) male mice as control group. Then, the Morris water maze (MWM) test, Y-maze spontaneous alternation test, open filed test and fear conditioning test were conducted for evaluation of learning and memory abilities. The DHA content was assessed by HPLC-MS/MS. Physiological indices were determined, including triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), ROS level, activity of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), PEG2, TXB2 and LTB4. The expressions of COX-1, COX-2, cPLA2, iPLA2, 15-LOX, and were assessed by Western blot.

Results: APP/PS1 mice showed serious cognitive impairment in behavioral tests. However, treatment of DSS extract significantly ameliorated the cognitive deficits of APP/PS1 mice. Biochemical measurements showed the increases in TG, TC, LDL-c and the decrease in HDL-c in APP/PS1 mice compared with WT mice, and DSS extract significantly retarded these changes. Low content of DHA, low expression of iPLA2 and 15-LOX were observed both in hippocampus and cortex of APP/PS1 mice, while DSS extract significantly restored these changes. Additionally, the abnormal activity of SOD and ROS level, the decreased levels of MDA and GSH were observed in APP/PS1 mice, while DSS extract prominently lessened these changes. Moreover, DSS extract decreased the level of PEG2, TXB2 and LTB4 and also attenuated the expression of cPLA2, COX-1 and COX-2 in hippocampus as well as cortex of APP/PS1 mice.

Conclusions: Based on these results, we suggest that DSS play a positive effective role in increasing DHA content by up-regulating iPLA2 and 15-LOX, resulting in ameliorating oxidative stress and inflammation and finally ameliorating cognition deficits in APP/PS1 mice.
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http://dx.doi.org/10.1016/j.jep.2020.112673DOI Listing
May 2020

Heat Stroke in Cell Tissues Related to Sulfur Dioxide Level Is Precisely Monitored by Light-Controlled Fluorescent Probes.

J Am Chem Soc 2020 02 29;142(6):3262-3268. Epub 2020 Jan 29.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science , Shanxi University , Taiyuan 030006 , China.

Heat stroke (HS) can cause serious organism damage or even death. Early understanding of the mechanism of heat cytotoxicity can prevent or treat heat stroke related diseases. In this work, probe was synthesized, characterized, and used for sulfur dioxide (SO) detection in lysosomes. PBS solutions of probe at pH 5.0 present a marked broad emission band in the green zone (535 nm). After UV irradiation, the spiropyran group in isomerizes to the merocyanine form (), which presented a weak red-shifted emission at 630 nm. In addition, photocontrolled isomerization of to generated a C═C-C═N fragment able to react, through a Michael addition, with SO to yield a highly emissive adduct with a marked fluorescence in the green channel (535 nm). In vitro studies showed a remarkable selectivity of photoactivated to SO with a limit of detection as low as 4.7 μM. MTT viability assays demonstrated that the is nontoxic to HeLa cells and can be used to detect SO in lysosomes. Taking advantage of this, the sensor is successfully applied to image increasing SO values in lysosomes during heat shock for the first time. Moreover, we also confirmed that the increased SO can protect the small intestine against damage induced by heat shock through regulating oxidative stress in cells and mice.
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http://dx.doi.org/10.1021/jacs.9b13936DOI Listing
February 2020

Seroprevalence of and in dairy cows in Hebei province, China.

Anim Biotechnol 2020 Jan 16:1-3. Epub 2020 Jan 16.

College of Life Science, Hebei Normal University, Shijiazhuang, China.

() and () are protozoan parasites that cause severe disease in animals and significant economic losses for farmers worldwide. is considered to be a serious parasite for dairy cows, while infection is a serious parasitic disease that can infect people and livestock. The aim of the present study was to investigate the seroprevalence of and in dairy cows in Hebei province, China. A total of 723 dairy cow sera samples from three regions in Hebei were analyzed by enzyme-linked immunosorbent assays (ELISA). The results revealed that 19.92% (144/723) of the individual serum samples were seropositive for , 37.34% (270/723) of the individual serum samples were seropositive for , and 8.7% (63/723) of the individual serum samples were seropositive for mixed infection. A higher seroprevalence of was found in the present study, when compared to previous data. To the best of the author's knowledge, the present study is the first to detect and antibodies in the vast areas of Hebei province, China.
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http://dx.doi.org/10.1080/10495398.2020.1714636DOI Listing
January 2020

Fast detecting hypochlorous acid based on electron-withdrawing group promoted oxidation and its biological applications in cells and root tips of plants.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Mar 28;229:118001. Epub 2019 Dec 28.

Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

Hypochlorous acid, a type of reactive oxygen species, has been shown to play an important role in organisms. Nowadays, there are many kinds of fluorescence detecting mechanisms to detect hypochlorous acid in vivo. Due to the high selectivity, the mechanism of using the strong oxidation of hypochlorous acid to break carbon‑carbon double bonds has been favored by many scientists. However, the reported probes of breaking carbon‑carbon double bonds still had drawback such as slow response. Based on this, we introduced electron-withdrawing group malonitrile to accelerate the oxidation of hypochlorous acid, resulting in reaction time less than 150 s. Meanwhile, the probe exhibited excellent selectivity, optical stability, high sensitivity and the detection limit as low as 0.19 μM. More importantly, we also successfully proved the potential application of the probe for the detection of intracellular ClO living cells and Arabidopsis root tip by fluorescence imaging.
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http://dx.doi.org/10.1016/j.saa.2019.118001DOI Listing
March 2020

A novel strategy: A consecutive reaction was used to distinguish in the presence of statins between normal cells and cancer cells.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Mar 23;229:117987. Epub 2019 Dec 23.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

Statins, as the most commonly drugs could reduce the concentration of low-density lipoprotein cholesterol, have been proved to elevate the HS generation in cells. Besides, the abnormal levels of biothiols might lead to cancer. Therefore, it is worth considering how to combine the characteristics of the two diseases to realize the detection of cancer cells. Based on this view, we developed a multiresponse fluorescent probe for the detection of hydrogen sulfide (HS) and biothiols successively based on theoretical calculation. It is interesting that the fluorescence intensity of the probe reacting HS and biothiols successively was significantly higher than that of probe reacting either of them. Based on this view, we further explored the biological application of the probe and found that the probe had obvious signal response to cancer cells than the normal cells in the presence of fluvastatin. This interesting finding might provide a new insight into cancer cell recognition.
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http://dx.doi.org/10.1016/j.saa.2019.117987DOI Listing
March 2020

A water-soluble fluorescent probe for the detection of thiophenols in water samples and in cells imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Mar 7;229:117905. Epub 2019 Dec 7.

Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. Electronic address:

Water pollution is the main cause of death of aquatic organisms such as fish et al. Content of thiophenols in water samples is an important indicator for assessing the degree of water pollution. The development of fluorescent probes with high selectivity and high sensitivity to detect thiophenols in water samples is extremely important in both environmental and life sciences. Although several fluorescent probes for thiophenols detection have been reported in recent years, most of them required the assistance of organic solvents to remedy the restriction caused by the poor water solubility of the probe, which did not fully reflect the actual situation of thiophenols in actual water samples. To fully overcome this shortage, we modified the 1,8-naphthylimide moiety with carboxyl to obtain a water-soluble fluorescent probe which could react with thiophenols specifically through nucleophilic aromatic substitution reaction (SAr) reaction with turn-on fluorescent responses. The corresponding detection limit was 71 nM. Supported by the spectroscopic changes, test strips based on the probe could detect thiophenols quantificationally and conveniently. At the same time, the probe could detect thiophenols in water sample with quantitative recovery. Besides, cell imaging experiments demonstrated the possibility of the probe to detect thiophenols in living cells.
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http://dx.doi.org/10.1016/j.saa.2019.117905DOI Listing
March 2020

A new strategy for the fluorescence discrimination of Cys/Hcy and GSH/HS simultaneously colorimetric detection for HS.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Feb 26;227:117537. Epub 2019 Oct 26.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China. Electronic address:

The development of fluorescent probes enabling distinguishable detection Cys, Hcy, GSH and HS is still a considerable challenge owing to their similar functional group with comparable reactivity. In this work, a novel fluorescent probe FHC-O-NBD has been synthesized, and a practicable strategy for the fluorescence discrimination of Cys/Hcy and GSH/HS, especially the colorimetric detection for HS have been presented. FHC-O-NBD reacted with Cys/Hcy to produce two fluorescent emissions at 486 nm and 550 nm, while for GSH/HS, only one fluorescent signal at 486 nm appeared. And, only upon addition of HS, the color of the system changed from colorless to pink. So it can serve as a colorimetric probe for HS by "naked eye". Furthermore, FHC-O-NBD can selectively distinguish Cys/Hcy and GSH/HS in living cells, meaning it has great potential in biological applications.
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http://dx.doi.org/10.1016/j.saa.2019.117537DOI Listing
February 2020

A Fluorescent Probe Based on Pyrene Ring for Detecting Cys and its Application in Biology.

J Fluoresc 2019 Sep 14;29(5):1241-1248. Epub 2019 Oct 14.

Research Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China.

The identification of thiols has become a research hotspot due to its role in biological systems. In this work, we simply constructed a turn-on fluorescent probe named 3-(5-bromopyridin-3-yl)-1-(pyren-1-yl) prop-2-en-1-one, that a combination of pyrene ring and substituted pyridine via the connection of α, β-unsaturated ketone. Cys can destroy the space effect by Michael addition reaction, which makes the fluorescence intensity changes. Furthermore, the probe featured excellent selectivity and high sensitivity (the detection limit was 0.52 μM) by addition of Cys. Moreover, this probe suggested a potential for imaging in vivo owing to the successful imaging of the probe in HepG2 cells, zebrafish, and Arabidopsis thaliana.
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http://dx.doi.org/10.1007/s10895-019-02441-wDOI Listing
September 2019

An Insight Into the Molecular Mechanism of Berberine Towards Multiple Cancer Types Through Systems Pharmacology.

Front Pharmacol 2019 6;10:857. Epub 2019 Aug 6.

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China.

Over the past several decades, natural products with poly-pharmacological profiles have demonstrated promise as novel therapeutics for various complex diseases, including cancer. Berberine (PubChem CID: 2353), a soliloquies quaternary alkaloid, has been validated to exert powerful effects in many cancers. However, the underlying molecular mechanism is not yet fully elucidated. In this study, we summarized the molecular effects of berberine against multiple cancers based on current available literatures. Furthermore, a systems pharmacology infrastructure was developed to discover new cancer indications of berberine and explore their molecular mechanisms. Specifically, we incorporated 289 high-quality protein targets of berberine by integrating experimental drug-target interactions (DTIs) extracted from literatures and computationally predicted DTIs inferred by network-based inference approach. Statistical network models were developed for identification of new cancer indications of berberine through integration of DTIs and curated cancer significantly mutated genes (SMGs). High accuracy was yielded for our statistical models. We further discussed three typical cancer indications (hepatocarcinoma, lung adenocarcinoma, and bladder carcinoma) of berberine with new mechanisms of actions (MOAs) based on our systems pharmacology framework. In summary, this study systematically provides a powerful strategy to identify potential anti-cancer effects of berberine with novel mechanisms from a systems pharmacology perspective.
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http://dx.doi.org/10.3389/fphar.2019.00857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691338PMC
August 2019

Discrete image recovery via stochastic resonance in optically induced photonic lattices.

Sci Rep 2019 Aug 14;9(1):11815. Epub 2019 Aug 14.

State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.

We demonstrate numerically the discrete image recovery via stochastic resonance in optically induced photonic lattices. The underlying signals are regularly reinforced at the expense of scattering noise with the interplay of the periodic potentials and the self-focusing nonlinearity. We founded that the energy redistribution tends to be periodic and the signal reinforcement is promoted with the help of periodic potentials. The lattice intensity levels, applied voltages, and correlation lengths are important parameters to influence the recovery effects. The dynamic nonlinear evolution including intensity and power spectrum is modeled according to the two-dimensional quasi-particle motion model. Our results suggest a potential technology to detect the noisy images.
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http://dx.doi.org/10.1038/s41598-019-48313-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694193PMC
August 2019