Publications by authors named "Yong-Mei Wang"

49 Publications

Leojaponin inhibits NLRP3 inflammasome activation through restoration of autophagy via upregulating RAPTOR phosphorylation.

J Ethnopharmacol 2021 Oct 9;278:114322. Epub 2021 Jun 9.

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Provincial Center for Research & Development of Natural Products, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China. Electronic address:

Ethnopharmacological Relevance: Duan Teng Yimu decoction is a Chinese herbal medicine compound with proven therapeutic effects on inflammasome-related diseases, such as rheumatoid arthritis. This decoction consists of three Chinese herbal medicines, including Leonurus japonicus (L. japonicus), which promotes the blood circulation and exhibits detumescence activity, traditionally curing gynecologic and inflammasome diseases.

Aim Of The Study: To explore the anti-inflammasome activity and the underlying mechanisms of action of the compounds from L. japonicus.

Materials And Methods: A series of compounds were isolated from L. japonicus. Their anti-inflammasome activities were evaluated in macrophages that were co-stimulated by lipopolysaccharide (LPS) and NLRP3 inflammasome inducers. NLRP3 inflammasome formation and apoptosis speck like containing a CARD (ASC) oligomerization were evaluated by immunofluorescent microscopy and Western blot analysis. The regulation of autophagy after treatment of this compound was also evaluated. Lastly, in vivo activity of Leojaponin was analyzed in a mouse acute gouty arthritis model.

Results: Here we show that Leojaponin, a diterpenoid compound from L. japonicus, suppressed lactate dehydrogenase and IL-1β release in Nigericin-stimulated macrophages in a pyroptosis model. Leojaponin inhibits NLRP3 inflammasome activation in both J774A.1 cells and bone marrow-derived macrophages in a dose dependent manner. Moreover, Leojaponin suppressed NLRP3-mediated ASC specks formation and ASC oligomerization. These activities of Leojaponin depend on restoration of autophagy via promoting RAPTOR phosphorylation. Furthermore, Leojaponin ameliorated monosodium urate (MSU)-induced acute gouty arthritis in vivo.

Conclusion: Our findings suggest that Leojaponin inhibits NLRP3 inflammasome activation through enhancing autophagy via RAPTOR phosphorylation, thereby highlighting Leojaponin as a potent drug for inflammasome-related diseases.
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http://dx.doi.org/10.1016/j.jep.2021.114322DOI Listing
October 2021

Multi-functional lanthanide coordination polymers for multi-modal detection of nitroaromatics and trace water in organic solvents.

J Colloid Interface Sci 2021 Sep 14;598:474-482. Epub 2021 Apr 14.

Academy of Advanced Interdisciplinary Research, School of Advanced Materials and Nanotechnology, Xidian University, Xi'an 710126, China. Electronic address:

Multifunctional materials used for multimodal sensors are of great significance both for enriching variety of materials and overcoming the shortcomings of traditional single signal sensors. This work reports the ultrafast preparation of multifunctional coordination polymer (CP) spheres with metalloligand Ru (II)[4,4'-(COOH)-bpy]▪Cl (L) and Gd ions. Gd-L CPs were used for multi-modal detection of nitroaromatic explosives and trace water in organic solvents with their red fluorescence and magnetic resonance response. The red fluorescence was derived from the ligand, which is electron-rich because of the bipyridine rings. There were different emission behaviors when Gd-L CPs reacted with nitroaromatic explosives that have various electron-deficient properties through electron transfer donor-acceptor mechanism. The changes in the emission depended closely on the nitroaromatic explosives ability to withdraw electrons. Gd-L CPs also showed magnetic resonance response with r of 12.1 mMs due to the integration of the paramagnetic property of Gd ions. Smart sensing of trace water in organic solvents was achieved by magnetic signals. The fluorescence of Gd-L CPs was enhanced and there was a tiny blue-shift in protonic solvents with increased polarity. The fluorescence sensor used in detecting trace water had a consistent result like the MR response. Thus, Gd-L CPs enabled the use of fluorescence and magnetic resonance sensors simultaneously for the first time to overcome the shortcoming of single modal sensor and expand the application of magnetic resonance relaxation.
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http://dx.doi.org/10.1016/j.jcis.2021.04.045DOI Listing
September 2021

Fabrication and application of biocompatible nanogenerators.

iScience 2021 Apr 5;24(4):102274. Epub 2021 Mar 5.

Academy of Advanced Interdisciplinary Research, School of Advanced Materials and Nanotechnology, Xidian University, Xi'an 710126, China.

As a new sustainable energy source, ubiquitous mechanical energy has received great attention and was successfully harvested by different types of nanogenerators. Among them, biocompatible nanogenerators are of particular interests due to their potential for biomedical applications. In this review, we provide an overview of the recent achievements in the fabrication and application of biocompatible nanogenerators. The development process and working mechanism of nanogenerators are introduced. Different biocompatible materials for energy harvesting, such as amino acids, peptide, silk protein, and cellulose, are discussed and compared. We then discuss different applications of biocompatible nanogenerators. We conclude with the challenges and potential research directions in this emerging field.
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http://dx.doi.org/10.1016/j.isci.2021.102274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010465PMC
April 2021

Sex-determining region Y-box 2-positive alveolar cells are responsive to bleomycin-induced lung injury.

Chin Med J (Engl) 2020 Sep 17;134(2):234-236. Epub 2020 Sep 17.

Department of Basic Medicine, Haihe Hospital, Tianjin University, Tianjin 300350, China.

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http://dx.doi.org/10.1097/CM9.0000000000001086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817345PMC
September 2020

Structurally Diverse Labdane Diterpenoids from and Their Anti-inflammatory Properties in LPS-Induced RAW264.7 Cells.

J Nat Prod 2020 09 16;83(9):2545-2558. Epub 2020 Sep 16.

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education; Yunnan Research & Development Center for Natural Products, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.

A phytochemical study on the aerial parts of led to the isolation and identification of 38 labdane diterpenoids, including 18 new (, , , , -, , -, , ) and 20 known (-, -, , , -, , ) analogues. Their structures were elucidated based on physical data analysis, including 1D and 2D NMR, HRMS, UV, IR, and X-ray diffraction. The structure of the known compound was confirmed by single-crystal X-ray diffraction data. These compounds can be divided into furanolabdane (-), tetrahydrofuranolabdane (-), lactonelabdane (-), labdane (-), and -labdane (-) type diterpenoids. All compounds were screened by lipopolysaccharide (LPS)-induced nitric acid (NO) production in RAW264.7 cells to evaluate anti-inflammatory effects. Compounds , , -, -, -, and inhibited NO production with IC values lower than 50 μM, with compound being the most active, with an IC value of 3.9 ± 1.7 μM. Further studies show that compound inhibits pro-inflammatory cytokine production and IKK α/β phosphorylation and restores the IκB expression levels in the NF-κB signaling pathway.
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http://dx.doi.org/10.1021/acs.jnatprod.9b00597DOI Listing
September 2020

Apelin-13 attenuates high glucose-induced calcification of MOVAS cells by regulating MAPKs and PI3K/AKT pathways and ROS-mediated signals.

Biomed Pharmacother 2020 Aug 22;128:110271. Epub 2020 May 22.

College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address:

Vascular calcification (VC) is an inducement of many cardiovascular diseases. Clinic evidences have confirmed that diabetes was the independent risk factor for VC, and the mechanism has not been well explored. Apelin as a ligand molecule is widely found in the cardiovascular system and showed potential in inhibiting VC, but the inhibitory effect and mechanism of apelin-13 against high glucose-induced VC have not been investigated yet. Herein, apelin-13 was employed to inhibit high glucose-induced VC in mouse aortic vascular smooth muscle cells (MOVAS), and the underlying mechanism was explored. The results showed that apelin-13 significantly inhibited high glucose-induced cells proliferation, migration and invasion of MOVAS cells. Apelin-13 also effectively attenuated high glucose-induced calcification by inhibiting alkaline phosphatase (ALP) activity and expression. Further investigation revealed that apelin-13 dramatically suppressed high glucose-induced DNA damage through inhibiting reactive oxide species (ROS) generation. Moreover, apelin-13 also effectively improved high glucose-induced dysfunction of MAPKs and PI3K/AKT. Inhibition of ERK by inhibitor (U0126) significantly blocked high glucose-induced calcification, which further confirmed the significance of MAPKs. Taken together, these results suggested that apelin-13 had the potential to attenuate high glucose-induced calcification of MOVAS cells by inhibiting ROS-mediated DNA damage and regulating MAPKs and PI3K/AKT pathways. Our findings validated the strategy of using apelin-13 maybe a novel way in treating high glucose-mediated VC.
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http://dx.doi.org/10.1016/j.biopha.2020.110271DOI Listing
August 2020

Nitrate Esters Alleviated Coronary Atherosclerosis Through Inhibition of NF-κB-Regulated Macrophage Polarization Shift in Epicardial Adipose Tissue.

J Cardiovasc Pharmacol 2020 05;75(5):475-482

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Nitrate esters have been used in clinical practice for more than one century for the treatment of angina. Their clinical effectiveness is due to vasodilator activity in arteries through a method of delivering nitric oxide or a nitric oxide-like compound. Recently, an increasing numbers of functions of this molecule in biology and pathophysiology have been discovered. Macrophage polarization shift in epicardial adipose tissue (EAT) has been demonstrated to be correlated with the severity of coronary artery disease (CAD). In this study, we aimed to investigate whether nitrate esters could improve coronary atherosclerosis through inhibition of macrophage polarization shift in EAT. A case-control study enrolled 48 subjects in 2 groups: CAD patients with or without nitrate esters treatment. Infiltration of M1/M2 macrophages and the expressions of pro-inflammatory and anti-inflammatory cytokines in EAT and subcutaneous white adipose tissue were investigated by immunohistochemical stain among subjects undergoing coronary artery bypass graft surgery. The expression levels of metabolic genes were investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). We found that nitrate ester treatment significantly inhibited NF-кB activity and decreased macrophage infiltration and M1/M2 macrophage ratio in EAT in patients with CAD. The expressions of pro-inflammatory cytokines were significantly decreased, along with significantly elevated expressions of anti-inflammatory cytokines in CAD patients with nitrate ester treatment, corresponding EAT dysfunction was ameliorated and the severity of patients with CAD (Gensini score) was significantly decreased. The protective effects on macrophage polarization and EAT function through NF-кB activity inhibition suggested a potential mechanism of nitrate esters in alleviating the severity of CAD.
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http://dx.doi.org/10.1097/FJC.0000000000000818DOI Listing
May 2020

Color-tunable lanthanide metal-organic framework gels.

Chem Sci 2019 Feb 28;10(6):1644-1650. Epub 2018 Nov 28.

State Key Laboratory of Medicinal Chemical Biology , Tianjin Key Laboratory of Biosensing and Molecular Recognition , College of Chemistry , Nankai University , Tianjin , 300071 , China . Email:

Fluorescence significantly improves the performance of gels. Various strategies, such as embedment and crosslinking, have been used to integrate extrinsic luminophores into gel systems, but the procedures are usually complex. Herein, for the first time, we report gels with intrinsic and tunable emission color prepared with 5-boronoisophthalic acid (5-bop) and Eu, Tb, and/or Dy similar to the procedure for the preparation of metal-organic frameworks (MOFs). The single-metal gels exhibit intrinsic trichromatic fluorescence, due to which full-color emissions are readily obtained by tuning the type and/or ratio of Ln ions to prepare mixed-metal gels. The emission is governed by an antenna effect and is thus excited with single-wavelength at 275 nm. The nucleation-growth mechanism reveals that the Ln ions and 5-bop produce separated layers, which then grow anisotropically to form nanoribbons by high coordinated valence of Ln ions and biased carboxyl distribution as well as steric hindrance and hydrogen bonds of the boric acid group in 5-bop. The nanoribbons entangle together to generate chemical-physical hybrid gels. To the best of our knowledge, this is the first example of gels with inherent and tunable emission color. Due to their optical and viscoelastic properties, the gels have numerous potential applications such as tunable emission and multi-target detection.
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http://dx.doi.org/10.1039/c8sc04732dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369438PMC
February 2019

Biventricular pacing for treating heart failure in children: A case report and review of the literature.

World J Clin Cases 2019 Feb;7(3):396-404

Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.

Background: Cardiac resynchronization therapy (CRT) can be used as an escalated therapy to improve heart function in patients with cardiac dysfunction due to long-term right ventricular pacing. However, guidelines are only targeted at adults. CRT is rarely used in children.

Case Summary: This case aimed to implement biventricular pacing in one child with heart failure who had a left ventricular ejection fraction < 35% at 4 years after implantation of an atrioventricular sequential pacemaker due to atrioventricular block. Postoperatively, echocardiography showed atrial sensing ventricular pacing and QRS wave duration of 120-130 ms, and cardiac function significantly improved after upgrading pacemaker.

Conclusion: Patients whose cardiac function is deteriorated to a level to upgrade to CRT should be upgraded to reverse myocardial remodeling as soon as possible.
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http://dx.doi.org/10.12998/wjcc.v7.i3.396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369388PMC
February 2019

Pharmacologic prevention of postoperative delirium after on-pump cardiac surgery: A meta-analysis of randomized trials.

Medicine (Baltimore) 2018 Oct;97(43):e12771

Department of Substance-Related Disorders, Division of Psychiatry, Hefei No.4 People's Hospital, Anhui Mental Health Center.

Background: Postoperative delirium is a prevalent and disabling mental disorder in patients undergoing on-pump cardiac surgery. There is some evidence that the use of pharmacological interventions may reduce the risk of developing of postoperative delirium. Therefore, the aim of this meta-analysis was to determine the effect of pharmacologic agents for the prevention postoperative delirium after cardiac surgery.

Methods: Randomized controlled trials (RCTs) were identified through a systematic literature search of electronic databases and article references up to October 2016. End points included incidence of postoperative delirium, severity of postoperative delirium, cognitive disturbances of postoperative delirium, duration of postoperative delirium, length of stay in intensive care unit (ICU) and hospital, and short-term mortality.

Results: A total of 14 RCTs with an aggregate of 14,139 patients were included. The results of the present meta-analysis show that pharmacologic agents significantly decrease postoperative delirium [relative risk (RR), 0.83; 95% confidence interval (95% CI), 0.75-0.91, P < .00001] and duration of postoperative delirium (RR = -0.37, 95% CI = -0.47 to -0.27, P < .00001) after on-pump cardiac surgery. In addition, subgroup analysis shows that dexamethasone and dexamethasone were associated with a trend toward a reduction in postoperative delirium (RR, 0.45; 95% CI, 0.30-0.66, P < .0001; RR, 0.80; 95% CI, 0.68-0.93, P = .003, respectively). However, our results fail to support the assumption that pharmacologic prophylaxis is associated with a positively reduction in short-term mortality, length of ICU, or hospital stay.

Conclusion: This meta-analysis suggests that the perioperative use of pharmacologic agents can prevent postoperative delirium development in patients undergoing cardiac surgery. However, there remain important gaps in the evidence base on a few small studies with multiple limitations. Further large-scale, high-quality RCTs are needed in this area.
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http://dx.doi.org/10.1097/MD.0000000000012771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6221693PMC
October 2018

Anticounterfeiting Quick Response Code with Emission Color of Invisible Metal-Organic Frameworks as Encoding Information.

ACS Appl Mater Interfaces 2018 Jul 21;10(26):22445-22452. Epub 2018 Jun 21.

State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry , Nankai University , Tianjin , 300071 , China.

Counterfeiting is a global epidemic that is compelling the development of new anticounterfeiting strategy. Herein, we report a novel multiple anticounterfeiting encoding strategy of invisible fluorescent quick response (QR) codes with emission color as information storage unit. The strategy requires red, green, and blue (RGB) light-emitting materials for different emission colors as encrypting information, single excitation for all of the emission for practicability, and ultraviolet (UV) excitation for invisibility under daylight. Therefore, RGB light-emitting nanoscale metal-organic frameworks (NMOFs) are designed as inks to construct the colorful light-emitting boxes for information encrypting, while three black vertex boxes were used for positioning. Full-color emissions are obtained by mixing the trichromatic NMOFs inks through inkjet printer. The encrypting information capacity is easily adjusted by the number of light-emitting boxes with the infinite emission colors. The information is decoded with specific excitation light at 275 nm, making the QR codes invisible under daylight. The composition of inks, invisibility, inkjet printing, and the abundant encrypting information all contribute to multiple anticounterfeiting. The proposed QR codes pattern holds great potential for advanced anticounterfeiting.
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http://dx.doi.org/10.1021/acsami.8b06901DOI Listing
July 2018

Lab-on-MOFs: Color-Coded Multitarget Fluorescence Detection with White-Light Emitting Metal-Organic Frameworks under Single Wavelength Excitation.

Anal Chem 2018 05 23;90(9):5758-5763. Epub 2018 Apr 23.

State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry , Nankai University , Tianjin , 300071 , China.

Multitarget assay with single detection modality from individual stimulation mode has aroused great attention. Here, we realize multitarget detection with the color change as signal source from single white light-emitting metal-organic frameworks (MOFs) under single wavelength excitation for the first time. The white light-emitting MOFs are prepared by carefully tuning the ratio of Eu, Tb, and Dy ions to obtain trimetal MOFs; 5-boronoisophthalic acid (5-bop) provides additional selectivity as ligand. Based on antenna effect, 5-bop is excited to produce its triplet-state, which sensitizes the Ln ions for the white emission. Thus, all of the fluorescence emission is achieved under single wavelength excitation at 275 nm, while any factors affecting the procedure result in the modulation of emission for diverse wavelengths. The trimetal MOFs display distinctive emission colors after interacting with different targets, including metal ions, anions, small molecule, and even biomolecule as a Lab-on-MOFs system. This system shows higher integration degree and simpler preparation and sensing procedures than other multitarget detection strategies.
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http://dx.doi.org/10.1021/acs.analchem.8b00086DOI Listing
May 2018

Multifunctional mixed-metal nanoscale coordination polymers for triple-modality imaging-guided photodynamic therapy.

Chem Sci 2017 May 10;8(5):3891-3897. Epub 2017 Mar 10.

State Key Laboratory of Medicinal Chemical Biology , Tianjin Key Laboratory of Biosensing and Molecular Recognition , College of Chemistry , Nankai University , Tianjin , 300071 , China . Email:

The one-step self-assembly preparation of multifunctional gadolinium (Gd)-ytterbium (Yb) mixed-metal nanoscale coordination polymers (NCPs) with Ru[4,4'-(COOH)bpy] ( , bpy = bipyridyl) as a ligand is reported. The Gd/Yb ratio in the NCPs is easily tuned by their ratio in the precursors while the self-limiting growth is realized with the high coordination valence and rigid steric structure of the precursors. The inherent properties of the precursors, including the magnetic resonance (MR) response of Gd, the X-ray attenuation properties of Yb, and the red fluorescence and the singlet-oxygen generation of , are well retained in the mixed-metal NCPs. fluorescence-MR-X-ray computed tomography (CT), triple-modality imaging and photodynamic therapy (PDT) are achieved using the mixed-metal NCPs as a probe. The triple-modality imaging integrates the high sensitivity of red fluorescence imaging, the deep penetration of MR imaging, and the 3D spatial resolution of CT imaging, thus providing comprehensive and complementary imaging information and facilitating the efficient imaging-guided PDT. For the first time, triple-modality imaging and a PDT agent were prepared with an easy and robust procedure, a tunable mixed-metal ratio, a high yield, and endogenous signal units.
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http://dx.doi.org/10.1039/c6sc05616dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465558PMC
May 2017

Highly oxygenated lanostane-type triterpenoids and their bioactivity from the fruiting body of Ganoderma gibbosum.

Fitoterapia 2017 Jun 11;119:1-7. Epub 2017 Mar 11.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, and State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China. Electronic address:

Eight new highly oxygenated lanostane triterpenes, gibbosic acids A-H (1-8), along with three known ones (9-11), were isolated from the fruiting body of Ganoderma gibbosum. The structures of new isolates were assigned by NMR and HRESIMS experiments. The absolute configurations of 1 were further confirmed by single crystal X-ray diffraction data and computational ECD methods. Immunoregulatory effect and anti-inflammatory activities of these compounds were screened in murine lymphocyte proliferation assay and in lipopolysaccharide (LPS)-stimulated RAW-264.7 macrophages, respectively. Compound 2 exhibited immunostimulatory effect both in lymphocyte proliferation assay without any induction and ConA-induced mitogenic activity of T-lymphocyte, and the proportion of lymphocyte proliferation at the concentration of 0.1μM are 20.01% and 21.40%, respectively.
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http://dx.doi.org/10.1016/j.fitote.2017.03.007DOI Listing
June 2017

Fluorescent Imaging-Guided Chemotherapy-and-Photodynamic Dual Therapy with Nanoscale Porphyrin Metal-Organic Framework.

Small 2017 05 28;13(17). Epub 2017 Feb 28.

State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, 300071, China.

Imaging-guided therapy systems (IGTSs) are revolutionary techniques used in cancer treatment due to their safety and efficiency. IGTSs should have tunable compositions for bioimaging, a suitable size and shape for biotransfer, sufficient channels and/or pores for drug loading, and intrinsic biocompatibility. Here, a biocompatible nanoscale zirconium-porphyrin metal-organic framework (NPMOF)-based IGTS that is prepared using a microemulsion strategy and carefully tuned reaction conditions is reported. A high content of porphyrin (59.8%) allows the achievement of efficient fluorescent imaging and photodynamic therapy (PDT). The 1D channel of the Kagome topology of NPMOFs provides a 109% doxorubicin loading and pH-response smart release for chemotherapy. The fluorescence guiding of the chemotherapy-and-PDT dual system is confirmed by the concentration of NPMOFs at cancer sites after irradiation with a laser and doxorubicin release, while low toxicity is observed in normal tissues. NPMOFs are established as a promising platform for the early diagnosis of cancer and initial therapy.
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http://dx.doi.org/10.1002/smll.201603459DOI Listing
May 2017

Cystic Bronchiectasis Imitating Pulmonary Aspergilloma.

Chin Med J (Engl) 2016 09;129(17):2137-8

Department of Basic Medicine, Tianjin Institute of Respiratory Diseases, Tianjin 300350; Department of Pathology, Tianjin Haihe Hospital, Tianjin 300350, China.

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http://dx.doi.org/10.4103/0366-6999.189051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009603PMC
September 2016

Two new phenolic compounds from the seeds of Machilus yunnanensis.

J Asian Nat Prod Res 2016 Oct 7;18(10):952-8. Epub 2016 Jun 7.

a State Key Laboratory of Phytochemistry and Plant Resources in West China , Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201 , PR China.

Chemical constituents investigation on the seeds of Machilus yunnanensis led to two new phenolic compounds 8-O-acetyl-phenylethanoid-4-O-β-D-glucopyranoside (1) and (E)-2,3-bis(4-hydroxyphenyl)acrylaldehyde (2), together with 16 known compounds. Their structures were elucidated on the basis of spectroscopic data analysis (IR, MS, 1D, and 2D NMR). Meanwhile, compounds 1-3, 6-13, 17, and 18 were evaluated for vasorelaxant effects on the rat endothelium-intact thoracic aorta rings precontracted with phenylephrine (PE) or KCl. The bioassay results showed that compound 17 had significant vasorelaxant effect on the endothelium-intact thoracic aorta rings precontracted with KCl.
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http://dx.doi.org/10.1080/10286020.2016.1187139DOI Listing
October 2016

Comparison of transducers with different frequencies in breast contrast-enhanced ultrasound (CEUS) using SonoVue as contrast agent.

Br J Radiol 2016 Jul 4;89(1063):20151050. Epub 2016 May 4.

1 Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan, China.

Objective: To explore the effectiveness of different transducers in breast contrast-enhanced ultrasound (CEUS) using SonoVue(®) (Bracco, Plan-Les-Ouates, Switzerland) as the contrast agent.

Methods: Breast CEUS was performed in 51 patients with 51 breast lesions using a low-frequency transducer (probe C5-1) and a high-frequency transducer (probe L12-5) separately. All image processes were reviewed for the presence of local blood perfusion defects and surrounding vessels. McNemar's test was conducted to compare the detection effectiveness between these two transducers.

Results: Pathological results revealed 38 malignant and 13 benign lesions. The two transducers showed no difference in detecting benign lesions. Among malignant lesions, CEUS conducted by probe C5-1 (frequency range from 1 to 5 MHz) presented 23 (60.5%) lesions with local blood perfusion defects and 26 (68.4%) lesions with surrounding vessels. Meanwhile, probe L12-5 (frequency range from 5 to 12 MHz) showed only 12 (31.6%) lesions with local blood perfusion defects and 12 (31.6%) lesions with surrounding vessel. Probe C5-1 was more sensitive than probe L12-5 in detecting malignant CEUS characteristics (p-value < 0.05).

Conclusion: The low-frequency transducer was more sensitive than the high-frequency transducer in breast CEUS using SonoVue as the contrast agent. A new contrast agent with a higher resonance frequency, specially designed for high-frequency transducers, may be helpful in improving the clinical value of breast CEUS.

Advances In Knowledge: The first study comparing different frequency transducers in breast CEUS of the same patient lesions. We brought out the requirement for CEUS contrast agents which are more suitable for high-frequency examinations.
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http://dx.doi.org/10.1259/bjr.20151050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5257312PMC
July 2016

Glycemic variability in normal glucose tolerance women with the previous gestational diabetes mellitus.

Diabetol Metab Syndr 2015 24;7:82. Epub 2015 Sep 24.

Department of Endocrinology, The Second Affiliated Hospital of Nantong University, No. 6 North Hai-er-xiang Road, Nantong, 226001 China.

Background: Women with previous gestational diabetes mellitus (pGDM) and postpartum normal glucose tolerance (NGT) may carry impaired islet β cell secretion, insulin resistance and subsequent altered glucose homeostasis. And certain normoglycemic groups at risks of diabetes were presented with elevated glycemic variability. The aim of study was to investigate the glycemic variability in NGT women with pGDM.

Methods: Total 48 NGT women with pGDM (pGDM group) and 48 age- and BMI-matched NGT women without pGDM (control group) were recruited in the study. Integrated β cell function was assessed with the Insulin Secretion-Sensitivity Index-2 (ISSI-2) derived from oral glucose tolerance test. All subjects were monitored using the continuous glucose monitoring system for consecutive 72 h. The multiple parameters of glycemic variability included the mean blood glucose (MBG), standard deviation of blood glucose (SDBG), mean of daily differences (MODD), mean amplitude of glycemic excursions (MAGE) and the incremental areas above preprandial glucose values (AUCpp).

Results: The pGDM group had a higher MBG (6.5 ± 0.9 vs. 5.9 ± 0.8 mmol/L, p < 0.05), SDBG (1.3 ± 0.3 vs. 0.9 ± 0.2 mmol/L, p < 0.05), MODD (1.4 ± 0.3 vs. 1.1 ± 0.2 mmol/L, p < 0.05), MAGE (2.7 ± 0.4 vs. 1.8 ± 0.5 mmol/L, p < 0.05), and AUCpp (26.8 ± 3.4 vs. 19.2 ± 3.2 mmol/L·h, p < 0.05), when compared to the control group, and the differences remained significant after adjusting for anthropometric indices and metabolic risk factors. Islet β cell function index ISSI-2 in the pGDM group was lower than in the control group (p < 0.05). MBG, SDBG, MODD, MAGE and AUCpp were all negatively associated with ISSI-2 in the pGDM group (r = -0.31, -0.30, -0.34, -0.48 and -0.54, respectively, p < 0.05), and the correlations remained significant after adjusting for anthropometric indices and metabolic risk factors.

Conclusions: Normal glucose tolerance women with pGDM were presented with elevated glycemic variability, which may be associated with impaired islet β cell function.
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http://dx.doi.org/10.1186/s13098-015-0077-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581077PMC
September 2015

Effect of vitamin D on clinical and biochemical parameters in polycystic ovary syndrome women: A meta-analysis.

J Obstet Gynaecol Res 2015 Nov 14;41(11):1791-802. Epub 2015 Sep 14.

Department of Thoracic Surgery, Hebei Chest Hospital, Shijiazhuang, China.

Aim: Using a meta-analysis framework, we investigated the association between the serum level of vitamin D and the risk of polycystic ovary syndrome (PCOS) and further examined the therapeutic effect of vitamin D on the clinical features of PCOS.

Material And Methods: Multiple databases were searched to retrieve studies. We chose clinical studies that investigated the relation between the serum level of vitamin D and the risk of PCOS or the therapeutic effect of vitamin D on PCOS. The search results were screened according to strict inclusion and exclusion criteria to select high-quality studies for inclusion. Statistical analyses were carried out using stata 12.0.

Results: Seventeen studies were eligible in this meta-analysis. The levels of 25-hydroxyvitamin D and the quantitative insulin-sensitivity check index in the PCOS group were remarkably lower than in the controls, whereas the homeostasis model assessment of insulin resistance in the PCOS group was markedly higher than in the controls. No statistically significant difference was observed in serum parathyroid hormone levels between the two groups. The 25-hydroxyvitamin D levels were significantly elevated after PCOS patients received vitamin D3 treatment, but serum parathyroid hormone concentration, homeostasis model assessment of insulin resistance and quantitative insulin-sensitivity check index did not show any significant changes, indicating a lack of therapeutic response.

Conclusion: Our results suggest that the serum level of vitamin D is associated with the risk of PCOS, but the therapeutic effect of vitamin D on PCOS remains to be further explored.
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http://dx.doi.org/10.1111/jog.12793DOI Listing
November 2015

Sitagliptin alleviated myocardial remodeling of the left ventricle and improved cardiac diastolic dysfunction in diabetic rats.

J Pharmacol Sci 2015 Mar 23;127(3):260-74. Epub 2014 Dec 23.

Department of Cardiology, The Qilu Hospital of Shandong University, Jinan, Shandong, 250033, PR China. Electronic address:

Objective: Sitagliptin, a dipeptidyl peptidase IV (DPP-Ⅳ) inhibitor, has a biological role in improving the serum levels of glucagon-like peptide 1 (GLP-1). Hence, we sought to determine the effect of sitagliptin on myocardial inflammation, collagen metabolism, lipid content and myocardial apoptosis in diabetic rats.

Materials And Methods: The type 2 diabetic rat model was induced by low-dose streptozotocin and a high-fat diet. Characteristics of diabetic rats were evaluated by electrocardiography, echocardiography and blood analysis. Cardiac inflammation, fibrosis, cardiomyocyte density, lipid accumulation, and receptor-interacting protein kinase 3 (RIP3) level, related to apoptosis, were detected by histopathologic analysis, RT-PCR and western blot analysis to evaluate the effects of sitagliptin on myocardial remodeling of the left ventricle.

Results: Diabetic rats showed myocardial hypertrophy or apoptosis, inflammation, lipid accumulation, myocardial fibrosis, elevated collagen content, RIP3 overexpression, and left-ventricular dysfunction. Sitagliptin could reverse the overexpression of RIP3 and alleviate cellular apoptosis in myocardial tissues. It could significantly improve left-ventricular systolic pressure and +dp/dt max, reduce the E/E' ratio, left ventricular end diastolic pressure, -dp/dt max and Tau in diabetic rats.

Conclusions: Sitagliptin might have a myocardial protective effect by inhibiting apoptosis, inflammation, lipid accumulation and myocardial fibrosis in diabetic rats, for a potential role in improving left-ventricular function in diabetes.
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http://dx.doi.org/10.1016/j.jphs.2014.12.007DOI Listing
March 2015

Systematic and endothelial inflammation and endothelial progenitor cell levels in emphysematous rats exposed to intermittent hypoxia.

Respir Care 2015 Feb 13;60(2):279-89. Epub 2015 Jan 13.

Respiratory Department, Tianjin Medical University General Hospital, Tianjin, China.

Background: This study aimed to develop an overlap syndrome rat model with intermittent hypoxia (IH) exposure as seen in obstructive sleep apnea, on a base of preexisting emphysema caused by 16 wk of smoke exposure to determine whether IH and emphysema existing simultaneously play overlapped roles on systematic/endothelial inflammation and endothelial damage.

Methods: Sixty male Wistar rats were divided into 4 groups of 15 each, labeled according to exposure conditions as control, IH, emphysema, and overlap groups. In these animals, electroencephalogram monitoring and preliminary experiments to obtain arterial blood gas values were performed. Serum concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-6, TNF-α and IL-6 concentrations in the culture medium, Ras homology A mRNA expression levels of endothelial cells from right common carotid artery, and ratio of carotid intima-media thickness of whole thickness of vascular wall expressed in percent (C-IMT) (%) values were evaluated. Subsequently, circulating endothelial progenitor cells (EPCs) within rat peripheral blood and bone marrow were measured with flow cytometry.

Results: The serum and endothelial concentrations of TNF-α and IL-6 and the levels of endothelial Ras homology A mRNA have statistically significant results described as overlap>emphysema>IH>control. The levels of EPCs in rat peripheral blood and bone marrow have statistically significant results described as overlap>IH>emphysema>control. C-IMT (%) values from right common carotid artery are the highest in the overlap group and the lowest in the control group. There is no statistical difference when comparing the IH and the emphysema groups.

Conclusions: Regardless of whether IH and emphysema exposure are mechanistically synergistic, this overlap elicits a more severe systematic/endothelial inflammation and endothelial damage; meanwhile, a robust mobilization of EPCs is demonstrated, which is not to mean a robust adherent and repairing capability.
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http://dx.doi.org/10.4187/respcare.03492DOI Listing
February 2015

One-pot organocatalytic enantioselective Michael/Povarov domino strategy for the construction of spirooctahydroacridine-3,3'-oxindole scaffolds.

Chemistry 2014 May 20;20(20):5899-904. Epub 2014 Mar 20.

State Key Laboratory of Elemento-Organic Chemistry, Department of Chemistry, Nankai University, Tianjin 300071 (China), Fax: (+86) 22-23504439.

An asymmetric organocatalytic one-pot strategy for the construction of spirooctahydroacridine-3,3'-oxindole scaffolds has been successfully developed by means of a domino Michael/Povarov reaction sequence. The one-pot protocol affords the chiral spirocyclohexaneoxindoles bearing an octahydroacridine motif with five stereocenters in good to high yields (up to 89 % yield) with excellent to perfect diastereoselectivities (up to >20:1 d.r.) and enantioselectivities (up to >99 % ee). This highly efficient one-pot domino procedure will allow diversity-oriented syntheses of this intriguing class of compounds with potential biological activities.
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http://dx.doi.org/10.1002/chem.201402002DOI Listing
May 2014

Circulating levels of β2-microglobulin and cystatin C are associated with left atrial size: additional link between the kidney and the heart.

Clin Nephrol 2013 Sep;80(3):168-76

Objective: More attention is being paid to the relationship between kidney dysfunction and cardiovascular events Characteristic features include renal dysfunction, left ventricular (LV) and left atrial (LA) enlargement. The aim of this study is to evaluate the relationships between circulating levels of β2-microglobulin (β2-m) and cystatin C and left atrial size in patients with coronary artery disease.

Materials And Methods: We recruited 300 patients who presented with chest tightness or chest pain and subsequently underwent coronary angiography. Of these, 202 patients were diagnosed with coronary artery disease (CAD) and 98 patients without CAD (non-CAD). Laboratory measurements included liver, kidney function (urea nitrogen, creatinine, β2-m and cystatin C), fasting glucose, and lipid analysis. CrCl were calculated according to Cockroft-Gault formula. Echocardiology was used to evaluate the cardiac structure and function.

Results: Significant differences of β2-m and cystatin C exist and no difference of creatinine and CrCl existed between the two groups. LA diameters were positively related to circulating levels of β2-m in the CAD group (r = 0.452, p < 0.001) and non-CAD group (r = 0.360, p < 0.001), and the similar relationships between LAD and circulating levels of cystatin C in the CAD group (r = 0.302, p < 0.001) and non-CAD group (r = 0.243, p = 0.016). LA diameters were negatively related to CrCl in both groups. After multivariate logistic regression analysis, the data indicated that the independent cardiovascular risk factors of LA enlargement for the patients with CAD were age, BMI, systolic blood pressure, LV mass, LVEDD, E/A, Em/Am, CrCl, and circulating levels of β2-m (OR = 1.630, 95% CI: 1.115 - 2.384, p = 0.012), cystatin C (OR = 4.504, 95% CI: 1.478 - 13.726, p = 0.008).

Conclusions: A linear correlation exists between circulating levels of β2-m or cystatin C and LA diameters. Higher circulating levels of β2-m or cystatin C are independent cardiovascular risk factors of LA enlargement in patients with CAD, and could be a link between the kidney and the heart.
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http://dx.doi.org/10.5414/CN107689DOI Listing
September 2013

Quantitative analysis of oleic acid and three types of polyethers according to the number of hydroxy end groups in Polysorbate 80 by hydrophilic interaction chromatography at critical conditions.

J Chromatogr A 2013 Jan 3;1272:73-80. Epub 2012 Dec 3.

Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, No. 188 Xingwan Rd, Nanchang 330004, China.

A quantitative characterization of Polysorbate 80 is crucial for its many applications. In this paper we report a quick RP-HPLC method for the quantitative determination of Polysorbate 80. The hydrolysis of Polysorbate 80 to release oleic acid and three types of polyethers was first carried out. A chromatographic method based on liquid chromatography at critical conditions (LCCC) was then developed for an endgroup-based separation of low-molecular-mass polyether. With this method the polyether, irrespective of its molecular-mass, is separated according to endgroups (functionality) due to interactions of the polar endgroups with the hydrophilic stationary phase. The different types of polyethers and oleic acid were identified using on-line electrospray ionization mass spectrometry and quantified by evaporative light scattering detection.
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http://dx.doi.org/10.1016/j.chroma.2012.11.070DOI Listing
January 2013

Highly efficient enantioselective construction of bispirooxindoles containing three stereocenters through an organocatalytic cascade Michael-cyclization reaction.

Chemistry 2013 Jan 18;19(5):1747-53. Epub 2012 Dec 18.

State Key Laboratory of Elemento-Organic Chemistry, Department of Chemistry, Nankai University, Tianjin 300071, PR China.

Bispirooxindole derivatives containing three stereocenters, including two spiro quaternary centers, were synthesized in a high-yielding, atypically rapid, and stereocontrolled cascade Michael-cyclization reaction between methyleneindolinones and isothiocyanato oxindoles catalyzed by a bi- or multifunctional organocatalyst. Mild conditions were used to construct bispirooxindoles with excellent enantio- and diastereomeric purities within less than 1 min. Catalyst reconfiguration offered access to the opposite enantiomer. This exceptionally highly efficient procedure will allow diversity-oriented syntheses of this intriguing class of compounds with potential biological activities.
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http://dx.doi.org/10.1002/chem.201203221DOI Listing
January 2013

[Inhibitory effects of thymoquinone on human pancreatic carcinoma orthotopically implanted in nude mice].

Authors:
Yong-Mei Wang

Zhonghua Yi Xue Za Zhi 2011 Nov;91(44):3111-4

Second Department of Medicine, Dezhou Municipal Hospital, Shandong, China.

Objective: To explore the anti-neoplastic and anti-metastatic effects of thymoquinone on the model of human pancreatic carcinoma established by surgical orthotopic implantation (SOI) in nude mice.

Methods: After a 3-week implantation, 40 mice were randomized into 4 groups: control group, feed with 1% ethanol; low-dose thymoquinone group (L-TQ 5 mg/kg BW daily by i.g.), high-dose thymoquinone group (H-TQ 20 mg/kg BW daily by i.g.) and gemcitabine group (GEM 100 mg/kg BW twice a week by i.p.) (n = 10 each). All treatment sessions lasted for 2 weeks. At Week 8 post-implantation, tumor weight, inhibition rate and the presence of metastasis were evaluated respectively after sacrificing. Immunohistochemistry was used to detect the positive expressions of Ki-67, XIAP (X-linked inhibitor of apoptosis protein) and MMP-9 (matrix metalloproteinase 9) in tumors.

Results: The tumor growth-inhibiting rates of L-TQ group, H-TQ group and GEM group were 42.57%, 64.11% and 54.77% respectively. The rates of metastasis in L-TQ group and H-TQ group were 60% and 50% versus GEM group (100%). And the differences had statistical significance (P < 0.05). The positive rates of Ki-67 in group L-TQ and group H-TQ were 37% ± 3% and 32% ± 2% versus group GEM (55% ± 4%). And the differences had statistical significance (P < 0.05). The positive rates of XIAP in group L-TQ and group H-TQ were 30% ± 3% and 28% ± 5% versus group GEM (56% ± 4%). And the differences had statistical significance (P < 0.05). The positive rates of MMP-9 in group L-TQ and group H-TQ were 57% ± 7% and 53% ± 2% versus group GEM (73% ± 4%). And the differences had statistical significance (P < 0.05).

Conclusion: Thymoquinone has the anti-neoplastic and anti-metastatic effects on the human pancreatic carcinoma in nude mice. And the above effects may be correlated with the down-regulated expressions of XIAP and MMP-9.
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November 2011

Association of lipoprotein-associated phospholipase A₂ with characteristics of vulnerable coronary atherosclerotic plaques.

Yonsei Med J 2011 Nov;52(6):914-22

Department of Cardiology, The Second Hospital of Shandong University, Jinan, China.

Purpose: Lipoprotein-associated phospholipase A₂ (Lp-PLA₂) is an inflammatory enzyme expressed in atherosclerotic plaques. We investigated the association of circulating Lp-PLA₂ with characteristics of vulnerable coronary atherosclerotic plaques.

Materials And Methods: We recruited 113 patients with either unstable angina (UA, n=59) and stable angina (SA, n=54) by coronary angiography. Thirty-six healthy subjects served as controls. Intravascular ultrasound (IVUS) was used to evaluate the characteristics of coronary atherosclerotic plaque, and serum Lp-PLA₂ concentration was measured as well.

Results: Lp-PLA₂ concentration was significantly higher in both UA and SA patients [(396±36) μg/L and (321±39) μg/L, respectively] compared with the controls [(127 ± 49) μg/L, p<0.01], and higher in UA than SA group. IVUS findings showed that remodeling index (RI) (0.91 ± 0.15 vs. 0.85 ± 0.11, p=0.005) and eccentricity index (EI) (0.73 ± 0.16 vs. 0.65 ± 0.22, p=0.039) were larger in UA than in SA group, and fibrous caps were thicker in SA than UA group [(0.91 ± 0.23) mm vs. (0.63 ± 0.21) mm, p=0.032]. Moreover, Lp-PLA₂ correlated positively with EI (r=0.439, p<0.01) and RI (r=0.592, p<0.05) in UA group. There was an inverse relationship between Lp-PLA₂ and fibrous cap thickness in both UA (r=-0.587, p<0.001) and SA (r=-0.318, p<0.05) groups. The independent risk factors in UA group were Lp-PLA₂ (OR=1.055, 95% CI: 1.03-1.08, p=0.013), LDL-cholesterol (OR=0.032, 95% CI: 0.00-0.05, p=0.041) and fibrous cap thickness (OR=0.008, 95% CI: 0.00-0.45, p=0.019). Lp-PLA₂ was strongly associated with both EI and fibrous cap thickness in both groups.

Conclusion: Serum level of Lp-PLA₂ is associated with both eccentricity index and fibrous cap thickness in both UA and SA groups. Elevated levels of circulating Lp-PLA₂ might to be a strong risk factor and more serious for unstable angina than stable angina.
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http://dx.doi.org/10.3349/ymj.2011.52.6.914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220251PMC
November 2011

[Spectral calibration of the atmosphere ultraviolet imaging spectrograph using a PtNe lamp].

Guang Pu Xue Yu Guang Pu Fen Xi 2010 Dec;30(12):3302-5

Lab for Space Environment Exploration, Center for Space Science and Applied Research, Chinese Academy of Sciences, Beijing 100190, China.

The atmospheric ultraviolet imaging spectrograph (AUVIS) is an ultraviolet imaging spectrograph that uses a two-dimensional charge-coupled device detector to collect both the spectrum and the swath perpendicular to the flight direction. The design of the system components, the principle, and the main specifications of the AUVIS are introduced. The calibration theory was studied. Considering the characteristics of the AUVIS, a PtNe hollow cathode lamp was chosen as the calibration source, a calibration setup was established, and the AUVIS was calibrated. After the experimental data processing, a function of pixel number-wavelength was established. The uncertainty of the spectral calibration is 0.043 nm.
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December 2010

Optimizing ultrasonic ellagic acid extraction conditions from infructescence of Platycarya strobilacea using response surface methodology.

Molecules 2010 Nov 4;15(11):7923-32. Epub 2010 Nov 4.

Institute of Chemical Industry of Forest Products, CAF, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. on Forest Chemical Engineering, SFA, Key Lab. of Biomass Energy and Material, Nanjing 210042, China.

The infructescence of Platycarya strobilacea is a rich source of ellagic acid (EA) which has shown antioxidant, anticancer and antimutagen properties. Response surface methodology (RSM) was used to optimize the conditions for ultrasonic extraction of EA from infructescence of P. strobilacea. A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. The content of EA in the extracts was determined by HPLC with UV detection. Three independent variables such as ultrasonic extraction temperature (°C), liquid:solid ratio (mL/g), and ultrasonic extraction time (min) were investigated. The experimental data obtained were fitted to a quadratic equation using multiple regression analysis and also analyzed by appropriate statistical methods. The 3-D response surface and the contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimum ultrasonic extraction conditions were as follows: ultrasonic extraction temperature 70 °C, liquid:solid ratio 22.5, and ultrasonic extraction time 40 min. Under these conditions, the experimental percentage value was 1.961%, which is in close agreement with the value predicted by the model.
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http://dx.doi.org/10.3390/molecules15117923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259309PMC
November 2010
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