Publications by authors named "Yong Zuo"

77 Publications

Glucose limitation activates AMPK coupled SENP1-Sirt3 signalling in mitochondria for T cell memory development.

Nat Commun 2021 07 16;12(1):4371. Epub 2021 Jul 16.

State Key Laboratory of Oncogenes and Related Genes, Renji Hospital Affiliated; Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Metabolic programming and mitochondrial dynamics along with T cell differentiation affect T cell fate and memory development; however, how to control metabolic reprogramming and mitochondrial dynamics in T cell memory development is unclear. Here, we provide evidence that the SUMO protease SENP1 promotes T cell memory development via Sirt3 deSUMOylation. SENP1-Sirt3 signalling augments the deacetylase activity of Sirt3, promoting both OXPHOS and mitochondrial fusion. Mechanistically, SENP1 activates Sirt3 deacetylase activity in T cell mitochondria, leading to reduction of the acetylation of mitochondrial metalloprotease YME1L1. Consequently, deacetylation of YME1L1 suppresses its activity on OPA1 cleavage to facilitate mitochondrial fusion, which results in T cell survival and promotes T cell memory development. We also show that the glycolytic intermediate fructose-1,6-bisphosphate (FBP) as a negative regulator suppresses AMPK-mediated activation of the SENP1-Sirt3 axis and reduces memory development. Moreover, glucose limitation reduces FBP production and activates AMPK during T cell memory development. These data show that glucose limitation activates AMPK and the subsequent SENP1-Sirt3 signalling for T cell memory development.
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http://dx.doi.org/10.1038/s41467-021-24619-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285428PMC
July 2021

Life History Recorded in the Vagino-cervical Microbiome Along with Multi-omics.

Genomics Proteomics Bioinformatics 2021 Jun 9. Epub 2021 Jun 9.

BGI-Shenzhen, Shenzhen 518083, China.

The vagina contains at least a billion microbial cells, dominated by lactobacilli. Here we perform metagenomic shotgun sequencing on cervical and fecal samples from a cohort of 516 Chinese women of reproductive age, and cervical, fecal, and salivary samples from a second cohort of 632 women. Factors such as pregnancy, delivery histories, cesarean section, and breast-feeding were all more important than menstrual cycle in shaping the microbiome, and such information would be necessary before trying to interpret differences between vagino-cervical microbiome data. Greater proportion of Bifidobacterium breve was seen with older age at sexual debut. The relative abundance of lactobacilli especially Lactobacillus crispatus was negatively associated with pregnancy history. Potential markers for lack of menstrual regularity, heavy flow, dysmenorrhea, and contraceptives were also identified. Lactobacilli were rare during breast-feeding or post-menopause. Other features such as mood fluctuations and facial speckles could potentially be predicted from the vagino-cervical microbiome. Gut and salivary microbiome, plasma vitamins, metals, amino acids, and hormones showed associations with the vagino-cervical microbiome. Our results offer an unprecedented glimpse into the microbiota of the female reproductive tract and call for international collaborations to better understand its long-term health impact other than in the settings of infection or pre-term birth.
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http://dx.doi.org/10.1016/j.gpb.2021.01.005DOI Listing
June 2021

P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint.

Cell Discov 2020 Apr 21;6(1):21. Epub 2020 Apr 21.

Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.

In response to DNA damage, p53-mediated signaling is regulated by protein phosphorylation and ubiquitination to precisely control G2 checkpoint. Here we demonstrated that protein SUMOylation also engaged in regulation of p53-mediated G2 checkpoint. We found that G2 DNA damage suppressed SENP3 phosphorylation at G2/M phases in p53-dependent manner. We further found that the suppression of SENP3 phosphorylation was crucial for efficient DNA damage/p53-induced G2 checkpoint and G2 arrest. Mechanistically, we identified Cdh1, a subunit of APC/C complex, was a SUMOylated protein at G2/M phase. SENP3 could de-SUMOylate Cdh1. DNA damage/p53-induced suppression of SENP3 phosphorylation activated SENP3 de-SUMOylation of Cdh. De-SUMOylation promoted Cdh1 de-phosphorylation by phosphatase Cdc14B, and then activated APC/C E3 ligase activity to ubiquitate and degrade Polo-like kinase 1 (Plk1) in process of G2 checkpoint. These data reveal that p53-mediated inhibition of SENP3 phosphorylation regulates the activation of Cdc14b-APC/C-Plk1 axis to control DNA damage-induced G2 checkpoint.
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http://dx.doi.org/10.1038/s41421-020-0154-2DOI Listing
April 2020

Single-Handed Double Helix and Spiral Platelet Formed by Racemate of Dissymmetric Cages.

Angew Chem Int Ed Engl 2021 Jun 26;60(27):15080-15086. Epub 2021 May 26.

Shanghai Key Laboratory of Electrical Insulation and Thermal Aging, Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.

Spontaneous deracemization has been used to separate homochiral domains from the racemic system. However, homochirality can only be referred to when the scales of these domains and systems are specified. To clarify this, we report self-assembly of racemates of dissymmetric cages DC-1 with a cone-shape propeller geometry, forming a centrosymmetric columnar crystalline phase (racemic at crystallographic level). Owing to their anisotropic geometry, the two enantiomers are packed in a frustrated fashion in this crystalline phase; single-handed double helices are observed (single-handedness at supramolecular level). The frustrated packing (layer continuity break-up) in turn facilitates screw dislocation during the crystal growth, forming left- or right-handed spiral platelets (symmetry-breaking at morphological level), although each platelet is composed of DC-1 racemates. The symmetry correlation between DC-1 molecules, the crystalline phase and spiral platelets, all exhibit C symmetry.
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http://dx.doi.org/10.1002/anie.202103821DOI Listing
June 2021

Transmission of a 112-Gbit/s 16-QAM over a 1440-km SSMF with parallel Kramers-Kronig receivers enabled by an overlap approach and bandwidth compensation.

Opt Express 2021 Mar;29(6):8117-8129

We investigate the parallelized performance of the conventional Kramers-Kronig (KK) and without the digital up-sampling KK (WDU-KK) receivers in a 112-Gbit/s 16-ary quadrature amplitude modulation (16-QAM) system over a 1440-km standard single-mode fiber (SSMF). A joint overlap approach and bandwidth compensation filter (OLA-BC) architecture is presented to mitigate the edge effect caused by the Hilbert transform and the Gibbs phenomenon induced by the FIR filter, respectively. Moreover, the computational complexity of the OLA-BC based parallelized KK/WDU-KK receiver is also discussed. Parallelized KK/WDU-KK receivers based on the presented OLA-BC architecture can effectively mitigate the edge effect and the Gibbs phenomenon together with more than two orders of magnitude improvement in terms of bit-error-ratio (BER) compared with parallelized KK/WDU-KK receivers without OLA-BC receivers in back-to-back (B2B) case. Finally, we successfully transmit the 16-QAM signals over 960-km SSMF with a BER lower than 7% hard-decision forward error correction (HD-FEC) threshold (3.8 × 10) and 1440-km SSMF with a BER lower than 20% soft-decision FEC (SD-FEC) threshold (2 × 10).
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http://dx.doi.org/10.1364/OE.413623DOI Listing
March 2021

Polar coded probabilistic shaping PAM8 based on many-to-one mapping for short-reach optical interconnection.

Opt Express 2021 Mar;29(7):10209-10220

In this paper, a polar coded probabilistic shaping (PS) 8-ary pulse amplitude modulation (PAM8) based on many-to-one (MTO) mapping is investigated for short-reach optical interconnection. By ingeniously assigning parity bits to ambiguities positions, no extra PS redundancy and no complex distribution matcher are required in the scheme comparing to traditional probabilistic amplitude shaping (PAS). The noise distributions after different transmission distances are studied and an optimal clock recovery method for PS signal is proposed to degrade the impact of severe eye skew effect on BER performance. The experimental results show that up to 1.2 dB and 0.8 dB shaping gains are respectively achieved over back-to-back (BTB) and 2-km standard single mode fiber (SSMF) transmission. With the help of the proposed optimal clock recovery method in the PS scheme, the shaping gain is improved from 0.15 dB to 0.4 dB after 10-km transmission. Moreover, compared to low-density parity-check (LDPC) code, the polar coded PS-PAM8 can provide an additional coding gain of 2.2 dB with code length of 256, which proves the performance superiority of polar code in short code length. Therefore, the proposed polar coded PS-PAM8 with low complexity and satisfactory BER performance is believed to be an alternative solution for the cost-sensitive short-reach optical interconnection.
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http://dx.doi.org/10.1364/OE.418045DOI Listing
March 2021

Large-area display textiles integrated with functional systems.

Nature 2021 03 10;591(7849):240-245. Epub 2021 Mar 10.

State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China.

Displays are basic building blocks of modern electronics. Integrating displays into textiles offers exciting opportunities for smart electronic textiles-the ultimate goal of wearable technology, poised to change the way in which we interact with electronic devices. Display textiles serve to bridge human-machine interactions, offering, for instance, a real-time communication tool for individuals with voice or speech difficulties. Electronic textiles capable of communicating, sensing and supplying electricity have been reported previously. However, textiles with functional, large-area displays have not yet been achieved, because it is challenging to obtain small illuminating units that are both durable and easy to assemble over a wide area. Here we report a 6-metre-long, 25-centimetre-wide display textile containing 5 × 10 electroluminescent units spaced approximately 800 micrometres apart. Weaving conductive weft and luminescent warp fibres forms micrometre-scale electroluminescent units at the weft-warp contact points. The brightness between electroluminescent units deviates by less than 8 per cent and remains stable even when the textile is bent, stretched or pressed. Our display textile is flexible and breathable and withstands repeated machine-washing, making it suitable for practical applications. We show that an integrated textile system consisting of display, keyboard and power supply can serve as a communication tool, demonstrating the system's potential within the 'internet of things' in various areas, including healthcare. Our approach unifies the fabrication and function of electronic devices with textiles, and we expect that woven-fibre materials will shape the next generation of electronics.
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http://dx.doi.org/10.1038/s41586-021-03295-8DOI Listing
March 2021

Effect of the Annealing Atmosphere on Crystal Phase and Thermoelectric Properties of Copper Sulfide.

ACS Nano 2021 Mar 1;15(3):4967-4978. Epub 2021 Mar 1.

Catalonia Energy Research Institute - IREC, Sant Adrià de Besòs, 08930 Barcelona, Spain.

CuS has become one of the most promising thermoelectric materials for application in the middle-high temperature range. Its advantages include the abundance, low cost, and safety of its elements and a high performance at relatively elevated temperatures. However, stability issues limit its operation current and temperature, thus calling for the optimization of the material performance in the middle temperature range. Here, we present a synthetic protocol for large scale production of covellite CuS nanoparticles at ambient temperature and atmosphere, and using water as a solvent. The crystal phase and stoichiometry of the particles are afterward tuned through an annealing process at a moderate temperature under inert or reducing atmosphere. While annealing under argon results in CuS nanopowder with a rhombohedral crystal phase, annealing in an atmosphere containing hydrogen leads to tetragonal CuS. High temperature X-ray diffraction analysis shows the material annealed in argon to transform to the cubic phase at . 400 K, while the material annealed in the presence of hydrogen undergoes two phase transitions, first to hexagonal and then to the cubic structure. The annealing atmosphere, temperature, and time allow adjustment of the density of copper vacancies and thus tuning of the charge carrier concentration and material transport properties. In this direction, the material annealed under Ar is characterized by higher electrical conductivities but lower Seebeck coefficients than the material annealed in the presence of hydrogen. By optimizing the charge carrier concentration through the annealing time, CuS with record figures of merit in the middle temperature range, up to 1.41 at 710 K, is obtained. We finally demonstrate that this strategy, based on a low-cost and scalable solution synthesis process, is also suitable for the production of high performance CuS layers using high throughput and cost-effective printing technologies.
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http://dx.doi.org/10.1021/acsnano.0c09866DOI Listing
March 2021

Effect of sevoflurane on iron homeostasis and toxicity in the brain of mice.

Brain Res 2021 04 2;1757:147328. Epub 2021 Feb 2.

Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050024, Hebei Province, China. Electronic address:

Sevoflurane (Sev), a commonly used volatile anesthetic, could induce nerve damage and cognitive deficiency. Oxidative stress induced by iron overload promotes nerve damage and cell apoptosis in the brain. This study revealed a new toxic mechanism of Sev to the brain occurred through the dysfunction of iron metabolism. Twelve-month-old C57BL/6 mice were randomly assigned to the following three groups: control group; 2% Sev (6 h) group; and Sev plus iron deficiency group. Iron levels and iron metabolism-related proteins and apoptosis-related factors in hippocampus and cortex tissues were detected by using synchrotron radiation micro-X-ray fluorescence (μ-XRF) and western blotting. Our results showed that a decline in cognitive function was observed in mice treated with Sev. Sev significantly induced iron accumulation through upregulating ferritin and downregulating transferrin receptor 1 which involved in ferroportin1 (Fpn1)/hepcidin pathway and increasing reactive oxygen species (ROS) and malondialdehyde (MDA) content of hippocampus and cortex. Sev aggravated BACE1 expression and Aβ accumulation. Changes in the ratio of Bcl2/Bax and Tau/p-Tau intensified the cell apoptosis in hippocampus and cortex tissues. Interestingly, the cognitive deficiency and neurotoxicity induced by Sev could be ameliorated significantly by feeding a low-iron diet to mice prior to anesthesia. The data uncovered a new lesion mechanism of Sev from the role of iron metabolism. This study also suggested that the reduction in iron levels could protect the brain against neurological damage induced by Sev.
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http://dx.doi.org/10.1016/j.brainres.2021.147328DOI Listing
April 2021

Improved polar decoding for optical PAM transmission via non-identical Gaussian distribution based LLR estimation.

Opt Express 2020 Dec;28(26):38456-38464

In this paper, an improved polar decoder based on non-identical Gaussian distributions is proposed and experimentally demonstrated for optical pulse amplitude modulation (PAM) interconnection. The principle of the polar coded PAM system is illustrated theoretically and the non-identical Gaussian distributions based log-likelihood ratio (LLR) estimation is introduced in the polar decoder to mitigate nonlinearity. Transmission systems of 28-Gbaud 4-level pulse amplitude modulation (PAM-4) and 8-level pulse amplitude modulation (PAM-8) based on commercial 10-GHz directly modulated laser (DML) are both demonstrated over 10-km standard single-mode fiber (SSMF) in C-band without dispersion compensation. Experimental results show that, aided by the improved polar decoder, the channel nonlinearity can be taken into consideration and additional sensitivity gains of 0.7 dB and 1 dB are respectively achieved compared with traditional polar decoder for PAM-4 and PAM-8 systems.
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http://dx.doi.org/10.1364/OE.403117DOI Listing
December 2020

Prenatal sevoflurane exposure: Effects of iron metabolic dysfunction on offspring cognition and potential mechanism.

Int J Dev Neurosci 2021 Feb 14;81(1):1-9. Epub 2020 Dec 14.

Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.

For decades, the neurotoxicity caused by anesthetics in mammalian brain development has gained increasing attention. Exposure to anesthetics leads to neurotoxicity and apoptosis of nerve cells, which in turn induces cognitive dysfunction. Although most of the data came from animal studies, general anesthetics have been shown to have adverse effects on cognitive function in infants and young children in recent years. This concern has led to a number of retrospective studies that observed an association between general anesthesia in pregnant women and neurobehavioral problems in fetuses or offspring. Every year, many pregnant women undergo non-obstetric anesthesia due to various reasons such as traffic accidents, fetal interventions, acute appendicitis, symptomatic cholelithiasis, and trauma. A matter of concern for these pregnant women is whether anesthesia has a detrimental effect on fetal brain development in the womb and whether the fetus has cognitive impairment after birth. In humans, the association of anesthetic exposure in infants with the long-term impairment of neurologic functions has been reported in several retrospective clinical studies. Recently, we have found that sevoflurane anesthesia during pregnancy in mice-induced cognitive impairment in the offspring by causing iron deficiency and inhibiting myelinogenesis. Sevoflurane is a commonly used general anesthetic in the hospitals, which can induce neurotoxicity and cause cognitive impairment in fetuses, infants, children, and adults. However, the exact mechanism of sevoflurane-induced damage to the central nervous system (CNS) is not fully understood. Based on our recent results, this paper reviewed the effects of sevoflurane on cognitive impairment and pathological changes such as neurogenesis, neuronal apoptosis, and iron metabolism dysfunction in the offspring.
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http://dx.doi.org/10.1002/jdn.10080DOI Listing
February 2021

Cardiomyocyte-specific deletion of Senp2 contributes to CVB3 viral replication and inflammation.

Int Immunopharmacol 2020 Nov 12;88:106941. Epub 2020 Sep 12.

Department of Children's Genetics and Infectious Diseases Laboratory, Dongguan Institute of Pediatrics, Dongguan, Guangdong 510000, China; Department of Respiratory Medicine, Dongguan Children's Hospital, Dongguan, Guangdong 510000, China. Electronic address:

Viral myocarditis (VMC) is characterized by cardiac inflammation and excessive inflammatory responses after viral infection. SENP2, a deSUMO-specific protease, has been reported to regulate antiviral innate immunity. This study aimed to investigate whether SENP2 affects CVB3-induced VMC. We generated a CVB3-induced VMC mouse model in 6-week-old cardiomyocyte-specific Senp2 knockout mice. The mice were sacrificed at days 0, 2, 4 and 6 after CVB3 infection. The survival rate, body weight, myocardial histopathological changes, viral load, cytokine levels and antiviral gene expression in cardiac tissues of both groups were investigated. Our study indicated that the expression of Senp2 in primary cardiomyocytes was upregulated by CVB3 infection. Moreover, deletion of Senp2 in the heart exacerbated CVB3 infection-induced myocarditis, facilitated CVB3 viral replication and downregulated the expression of antiviral proteins. In conclusion, our findings suggest a protective role for SENP2 in CVB3-induced VMC.
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http://dx.doi.org/10.1016/j.intimp.2020.106941DOI Listing
November 2020

Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages.

Cell Death Dis 2020 10 30;11(10):934. Epub 2020 Oct 30.

Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Acute respiratory distress syndrome (ARDS) is common in intensive care units (ICUs), although it is associated with high mortality, no effective pharmacological treatments are currently available. Despite being poorly understood, the role of programmed cell death protein 1 (PD-1) and PD-ligand 1 (PD-L1) axis in ARDS may provide significant insights into the immunosuppressive mechanisms that occur after ARDS. In the present study, we observed that the level of soluble PD-L1 (sPD-L1), a potential activator of the PD-1 pathway, was upregulated in survivors of direct ARDS than in non-survivors. Administration of sPD-L1 in mice with direct ARDS relieved inflammatory lung injury and improved the survival rate, indicating the protective role of sPD-L1 in direct ARDS. Using high-throughput mass cytometry, we found a marked decrease in the number of lung monocyte-derived macrophages (MDMs) with proinflammatory markers, and the protective role of sPD-L1 diminished in ARDS mice with monocyte/macrophage depletion. Furthermore, PD-1 expression increased in the MDMs of patients and mice with direct ARDS. Finally, we showed that sPD-L1 induced MDM apoptosis in patients with direct ARDS. Taken together, our results demonstrated that the engagement of sPD-L1 on PD-1 expressing macrophages resulted in a decrease in pro-inflammatory macrophages and eventually improved direct ARDS. Our study identified a prognostic indicator for patients with direct ARDS and a potential target for therapeutic development in direct ARDS.
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http://dx.doi.org/10.1038/s41419-020-03139-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596316PMC
October 2020

Selective Methanol-to-Formate Electrocatalytic Conversion on Branched Nickel Carbide.

Angew Chem Int Ed Engl 2020 Nov 9;59(47):20826-20830. Epub 2020 Sep 9.

Molecular Electrochemistry Laboratory, Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, P. R. China.

A methanol economy will be favored by the availability of low-cost catalysts able to selectively oxidize methanol to formate. This selective oxidation would allow extraction of the largest part of the fuel energy while concurrently producing a chemical with even higher commercial value than the fuel itself. Herein, we present a highly active methanol electrooxidation catalyst based on abundant elements and with an optimized structure to simultaneously maximize interaction with the electrolyte and mobility of charge carriers. In situ infrared spectroscopy combined with nuclear magnetic resonance spectroscopy showed that branched nickel carbide particles are the first catalyst determined to have nearly 100 % electrochemical conversion of methanol to formate without generating detectable CO as a byproduct. Electrochemical kinetics analysis revealed the optimized reaction conditions and the electrode delivered excellent activities. This work provides a straightforward and cost-efficient way for the conversion of organic small molecules and the first direct evidence of a selective formate reaction pathway.
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http://dx.doi.org/10.1002/anie.202004301DOI Listing
November 2020

Amphiphilic Organic Cages: Self-Assembly into Nanotubes and Enhanced Anion-π Interactions.

Chempluschem 2020 05;85(5):906-909

Frontiers Science Centre for Transformative Molecules Shanghai Key Laboratory of Electrical Insulation and Thermal Aging School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, P. R. China.

An amphiphilic organic cage was synthesized and used as self-assembly synthon for the fabrication of novel functional supramolecular structures in solution. The transmission electron microscopy (TEM) results showed that this amphiphilic cage self-assembled in aqueous solution into unilamellar nanotubes with a diameter of 29±4 nm at a concentration of 0.05 mg mL . Interestingly, the self-assembly of this cage significantly enhanced the anion-π interactions as indicated by a remarkable increasement of association constant (K ) between Cl and this amphiphilic cage after self-assembly. In specific, K was increased from 223 M for discrete cages in methanol to 6800 M for aggregated cages after self-assembly in water at the same concentration of 2.26×10  M. A mechanism based on a synergistic effect was proposed in order to explain this self-assembly process through enhanced anion-π interactions.
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http://dx.doi.org/10.1002/cplu.202000143DOI Listing
May 2020

P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint.

Cell Discov 2020 21;6:21. Epub 2020 Apr 21.

1Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China.

In response to DNA damage, p53-mediated signaling is regulated by protein phosphorylation and ubiquitination to precisely control G2 checkpoint. Here we demonstrated that protein SUMOylation also engaged in regulation of p53-mediated G2 checkpoint. We found that G2 DNA damage suppressed SENP3 phosphorylation at G2/M phases in p53-dependent manner. We further found that the suppression of SENP3 phosphorylation was crucial for efficient DNA damage/p53-induced G2 checkpoint and G2 arrest. Mechanistically, we identified Cdh1, a subunit of APC/C complex, was a SUMOylated protein at G2/M phase. SENP3 could de-SUMOylate Cdh1. DNA damage/p53-induced suppression of SENP3 phosphorylation activated SENP3 de-SUMOylation of Cdh. De-SUMOylation promoted Cdh1 de-phosphorylation by phosphatase Cdc14B, and then activated APC/C E3 ligase activity to ubiquitate and degrade Polo-like kinase 1 (Plk1) in process of G2 checkpoint. These data reveal that p53-mediated inhibition of SENP3 phosphorylation regulates the activation of Cdc14b-APC/C-Plk1 axis to control DNA damage-induced G2 checkpoint.
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http://dx.doi.org/10.1038/s41421-020-0154-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171148PMC
April 2020

Robust DNA-Bridged Memristor for Textile Chips.

Angew Chem Int Ed Engl 2020 07 25;59(31):12762-12768. Epub 2020 May 25.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, and Laboratory of Advanced Materials, Fudan University, Shanghai, 200438, China.

Electronic textiles may revolutionize many fields, such as communication, health care and artificial intelligence. To date, unfortunately, computing with them is not yet possible. Memristors are compatible with the interwoven structure and manufacturing process in textiles because of its two-terminal crossbar configuration. However, it remains a challenge to realize textile memristors owing to the difficulties in designing advanced memristive materials and achieving high-quality active layers on fiber electrodes. Herein we report a robust textile memristor based on an electrophoretic-deposited active layer of deoxyribonucleic acid (DNA) on fiber electrodes. The unique architecture and orientation of DNA molecules with the incorporation of Ag nanoparticles offer the best-in-class performances, e.g., both ultra-low operation voltage of 0.3 V and power consumption of 100 pW and high switching speed of 20 ns. Fundamental logic calculations such as implication and NAND are demonstrated as functions of textile chips, and it has been thus integrated with power-supplying and light emitting modules to demonstrate an all-fabric information processing system.
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http://dx.doi.org/10.1002/anie.202004333DOI Listing
July 2020

Sevoflurane anesthesia during pregnancy in mice induces cognitive impairment in the offspring by causing iron deficiency and inhibiting myelinogenesis.

Neurochem Int 2020 05 6;135:104693. Epub 2020 Feb 6.

Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, 050024, Hebei Province, China. Electronic address:

Maternal anesthetic exposure during pregnancy is associated with an increased risk of cognitive impairment in offspring. The balance of cerebral iron metabolism is essential for the development of brain tissue. Iron deficiency affects the myelinogenesis and nerve tissue development, especially in fetus or infant, which has a key role in cognitive function. We aimed to investigate whether maternal sevoflurane (Sev) exposure caused cognitive impairment in offspring through inducing iron deficiency and inhibiting myelinogenesis. Pregnant mice (gestation stage day 14) were treated with 2% Sev for 6 h. Cognitive function of offspring mice was determined by the Morris water maze and Context fear conditioning test. Iron levels were assayed by Perl's iron staining and synchrotron imaging. Hippocampus and cortex tissues or cerebral microvascular endothelial cells of offspring mice (postnatal day 35) were harvested and subjected to Western blot and/or immunhistochemistry to assess ferritin, transferrin receptor 1(TfR1), Ferroportin-1 (FpN1), myelin basic protein (MBP), tight junction protein ZO-1, occludin, and claudin-5 levels. Beginning with postnatal day 30, the offspring were treated with iron therapy for 30 days, and the indicators above were tested. Our results showed Sev dramatically decreased the iron levels of brain and impaired cognitive function in offspring mice. Sev decreased the expression of heavy chain ferritin (FtH), light chain ferritin (FtL), MBP, ZO-1, occludin, claudin-5, and FpN1, and increased TfR1 in hippocampus and cortex or cerebral microvascular endothelial cells of offspring mice, indicating that Sev caused the iron deficiency and impaired the myelinogenesis in the brain of offspring. Interestingly, iron therapy prompted the myelinogenesis and improved impaired cognitive function at postnatal day 60. Our research uncovered a new mechanism which showed that iron deficiency induced by Sev and myelin formation disorder due to decreased iron of brain may be an important risk factor for cognitive impairment in offspring. It was necessary for offspring to be supplied iron supplement whose mother suffered exposure to sevoflurane during pregnancy.
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http://dx.doi.org/10.1016/j.neuint.2020.104693DOI Listing
May 2020

Monodisperse CoSn and NiSn Nanoparticles Supported on Commercial Carbon as Anode for Lithium- and Potassium-Ion Batteries.

ACS Appl Mater Interfaces 2020 Jan 17;12(4):4414-4422. Epub 2020 Jan 17.

Catalonia Institute for Energy Research - IREC , Sant Adrià de Besòs , 08930 Barcelona , Spain.

Monodisperse CoSn and NiSn nanoparticles were prepared in solution and supported on commercial carbon black. The obtained nanocomposites were applied as anodes for Li- and K-ion batteries. [email protected] delivered stable average capacities of 850, 650, and 500 mAh g at 0.2, 1.0, and 2.0 A g, respectively, well above those of commercial graphite anodes. The capacity of [email protected] retained up to 575 mAh g at a current of 1.0 A g over 200 continuous cycles. Up to 74.5 and 69.7% pseudocapacitance contributions for Li-ion batteries were measured for [email protected] and [email protected], respectively, at 1.0 mV s. [email protected] was further tested in full-cell lithium-ion batteries with a LiFePO cathode to yield a stable capacity of 350 mAh g at a rate of 0.2 A g. As electrode in K-ion batteries, [email protected] composites presented a stable capacity of around 200 mAh g at 0.2 A g over 400 continuous cycles, and [email protected] delivered a lower capacity of around 100 mAh g over 300 cycles.
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http://dx.doi.org/10.1021/acsami.9b16418DOI Listing
January 2020

Realization of linear-mapping between polarization Poincaré sphere and orbital Poincaré sphere based on stress birefringence in the few-mode fiber.

Opt Express 2019 Nov;27(24):35537-35547

Based on the spatial profiles and polarization states evolution process of the first-order modes resulted from stress-induced birefringence in the few-mode fiber (FMF), we analyze the mapping relationship between the input polarization states represented on polarization PS and the output spatial profiles represented on the orbital PS of the FMF with respect to the magnitude and orientation of birefringence. When the input mode lobe orientation and the phase differences between the four eigenmodes of FMF induced by the stress birefringence satisfy a given condition, the mapping relationship between the input polarization PS and the output orbital PS is linear. Thus, the arbitrary points on the orbit PS can be generated at the output of stressed FMF by controlling the polarization state of the input modes. Then we experimentally verify that, an electrical single-mode polarization controller, a mode converter for converting fundamental mode to higher-order mode, a polarization controller mounting a coil of two-mode fiber and a polarizer can be employed to generate arbitrary first-order spatial modes on the orbital PS by controlling the input single-mode polarization states. The positions on the orbital PS of the generated first-order modes, which are obtained by calculating the three normalized Stokes parameters of output modes, agree well with the simulation ones. The correlation coefficients between the theoretical mode profiles and the experimental ones are higher than 80%. Since the spatial profile evolutions depend on the variations of the input polarization states, a potential advantage of this method is high-speed switching among desired first-order modes by using the commercial devices switching the state of polarization.
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http://dx.doi.org/10.1364/OE.27.035537DOI Listing
November 2019

Spatially correlated MIMO for exploiting the capacity of NLOS ultraviolet turbulent channels.

Opt Express 2019 Oct;27(21):30639-30652

In order to break through the inherent limitation of channel capacity, we employ a correlated multiple input multiple output (MIMO) framework over non-line-of-sight (NLOS) ultraviolet (UV) turbulent channels. Subsequently, a method of segmented correlation is proposed to define the spatial correlation of UV MIMO structures. Furthermore, the correlation and channel capacity are studied with varying elevation angle, turbulence intensity, signal-to-noise ratio (SNR) and number of transceiver in pairs (NTPs). The results show that this correlated MIMO framework can obtain higher channel capacity by reducing the elevation angle of the transceivers or increasing turbulence intensity or NTPs. Moreover, channel capacity increases with the SNR. But at low SNR, a larger gain of channel capacity can be provided. Therefore, this paper develops the capacity of UV turbulent channels and provides a guide for optimal parameter configuration of correlated UV MIMO.
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http://dx.doi.org/10.1364/OE.27.030639DOI Listing
October 2019

Propofol prevents oxidative stress and apoptosis by regulating iron homeostasis and targeting JAK/STAT3 signaling in SH-SY5Y cells.

Brain Res Bull 2019 11 28;153:191-201. Epub 2019 Aug 28.

Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050024, Hebei Province, China. Electronic address:

The present study aimed to test the hypothesis that propofol (PRO) could exert a neuroprotective effect via inhibiting oxidative stress induced by iron accumulation. Human SH-SY5Y cells were pretreated with ferric citrate (FAC), and then were protected by PRO. Cell viability was measured by MTT method. Iron levels were assayed by ICP-MS. Cell apoptosis was examined by TUNEL and digital holographic technique. Malondialdehyde (MDA), reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) depolarization were measured by MDA, DCFH-DA and JC-1 kits, respectively. The expression of proteins or genes involved in iron metabolism such as ferritin, TfR1, DMT1, Fpn1 and hepcidin, and other apoptosis-related proteins including Bcl2, Bax, Bid, Cox2, IL-6, JAK1 and STAT3 were detected by western blot. Our results showed low concentration of PRO (5 μM) could significantly prevent FAC induced apoptosis via inhibiting oxidative stress and iron accumulation. PRO suppressed the increase of ROS and MDA and decrease of MMP induced by FAC. PRO significantly down-regulated the expression of ferritin and up-regulated the expression of TfR1and Fpn1, but had no effect of DMT1. Furthermore, this effect was not done by PRO chelating iron. Meanwhile, PRO suppressed the inflammatory response through inhibiting IL-6 and Cox2 expression and activating JAK/STAT3 signaling induced by iron overload. In conclusion, here we demonstrated a new antioxidation mechanism of PRO. PRO could protect against nerve cell injury induced by overload of iron through regulating iron metabolism and inhibiting stress oxidative and inflammation reaction pathways by targeting JAK/STAT3 signaling.
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http://dx.doi.org/10.1016/j.brainresbull.2019.08.018DOI Listing
November 2019

SENP2 Suppresses Necdin Expression to Promote Brown Adipocyte Differentiation.

Cell Rep 2019 08;28(8):2004-2011.e4

State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, Shanghai 200025, China; Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address:

Brown adipose tissue (BAT) is a thermogenic organ that maintains body temperature and energy homeostasis. Transcriptional regulation plays an important role in the program of brown adipogenesis. However, it remains unclear how the transcriptional events are controlled in this program. In this study, we analyze an SENP2 BAT conditional knockout mouse model and find that SENP2-mediated de-SUMOylation is essential for BAT development. SENP2 catalyzes de-SUMOylation of cAMP response element-binding protein (CREB) to suppress Necdin expression, which induces brown adipocyte differentiation and brown adipogenesis. Mechanistically, we find that SUMOylation enhances CREB interaction with serine/threonine protein phosphatase 2A (PP2A) to de-phosphorylate CREB, which activates Necdin transcription. SENP2 deficiency enhances the expression of Necdin to inhibit brown adipocyte differentiation. Therefore, we reveal a crucial role of SENP2-mediated de-SUMOylation of CREB in suppression of Necdin expression during brown adipose development and brown adipogenesis.
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http://dx.doi.org/10.1016/j.celrep.2019.07.083DOI Listing
August 2019

SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.

Mol Cell 2019 08 10;75(4):823-834.e5. Epub 2019 Jul 10.

Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; State Key Laboratory of Oncogenes and Related Genes, Renji Hospital Affiliated, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China. Electronic address:

Sirt3, as a major mitochondrial nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, is required for mitochondrial metabolic adaption to various stresses. However, how to regulate Sirt3 activity responding to metabolic stress remains largely unknown. Here, we report Sirt3 as a SUMOylated protein in mitochondria. SUMOylation suppresses Sirt3 catalytic activity. SUMOylation-deficient Sirt3 shows elevated deacetylation on mitochondrial proteins and increased fatty acid oxidation. During fasting, SUMO-specific protease SENP1 is accumulated in mitochondria and quickly de-SUMOylates and activates Sirt3. SENP1 deficiency results in hyper-SUMOylation of Sirt3 and hyper-acetylation of mitochondrial proteins, which reduces mitochondrial metabolic adaption responding to fasting. Furthermore, we find that fasting induces SENP1 translocation into mitochondria to activate Sirt3. The studies on mice show that Sirt3 SUMOylation mutation reduces fat mass and antagonizes high-fat diet (HFD)-induced obesity via increasing oxidative phosphorylation and energy expenditure. Our results reveal that SENP1-Sirt3 signaling modulates Sirt3 activation and mitochondrial metabolism during metabolic stress.
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http://dx.doi.org/10.1016/j.molcel.2019.06.008DOI Listing
August 2019

SIRT3 deacetylase activity confers chemoresistance in AML via regulation of mitochondrial oxidative phosphorylation.

Br J Haematol 2019 10 24;187(1):49-64. Epub 2019 Jun 24.

Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Acute myeloid leukaemia (AML) cells possess metabolism profiles, such as higher rates of oxidative phosphorylation and dependence on fatty acid oxidation for survival, and are dependent on the sophisticated regulation of reactive oxygen species (ROS) generation for survival, drug resistance and stemness maintenance. We found that sensitivity of primary AML cells to cytarabine correlated with SOD2 acetylation and the ability of the drug to induce mitochondrial ROS. The SOD2 deacetylase, SIRT3, protected AML cells from chemotherapy as shown by inhibited apoptosis via inhibited drug-induced production of mitochondrial ROS. SIRT3 significantly decreased nicotinamide adenine dinucleotide phosphate (NADP)/reduced NADP ratio and increased reduced glutathione/oxidized glutathione ratio. Furthermore, SIRT3 enhanced oxidative phosphorylation (OxPhos) in AML cells under both basic and cytarabine-treated conditions. A xenograft mouse model showed that SIRT3 overexpressing AML cells and patient-derived xenograft mice bearing high SIRT3 deacetylase activity were more resistant to chemotherapy in vivo. SIRT3 inhibitor displayed synergy with cytarabine to ablate AML cells in vitro and in mouse models. Taken together, our study showed that SIRT3 is capable of reprograming mitochondrial metabolism towards OxPhos and downregulating ROS generation, which contribute to the chemoresistance of AML cells. SIRT3 can be utilized as a potential therapeutic target to improve the anti-leukaemic efficacy of standard chemotherapeutic agents for AML.
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http://dx.doi.org/10.1111/bjh.16044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790595PMC
October 2019

SUMO-Specific Protease 1 Is Critical for Myeloid-Derived Suppressor Cell Development and Function.

Cancer Res 2019 08 11;79(15):3891-3902. Epub 2019 Jun 11.

Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Myeloid-derived suppressor cells (MDSC) can suppress immunity and promote tumorigenesis, and their abundance is associated with poor prognosis. In this study, we show that SUMO1/sentrin-specific peptidase 1 (SENP1) regulates the development and function of MDSC. SENP1 deficiency in myeloid cells promoted MDSC expansion in bone marrow, spleen, and other organs. MDSC showed stronger immunosuppressive activity than MDSC; we observed no defects in the differentiation of myeloid precursor cell in mice. Mechanistically, SENP1-mediated regulation of MDSC was dependent on STAT3 signaling. We identified CD45 as a specific STAT3 phosphatase in MDSC. CD45 was SUMOylated in MDSC and SENP1 could deconjugate SUMOylated CD45. In MDSC, CD45 was highly SUMOylated, which reduced its phosphatase activity toward STAT3, leading to STAT3-mediated MDSC development and function. These results reveal a suppressive function of SENP1 in modulating MDSC expansion and function via CD45-STAT3 signaling axis. SIGNIFICANCE: These findings show that increased SUMOylation of CD45 via loss of SENP1 suppresses CD45-mediated dephosphorylation of STAT3, which promotes MDSC development and function, leading to tumorigenesis.
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http://dx.doi.org/10.1158/0008-5472.CAN-18-3497DOI Listing
August 2019

Transcriptomic analysis reveals the molecular mechanism of Alzheimer-related neuropathology induced by sevoflurane in mice.

J Cell Biochem 2019 10 27;120(10):17555-17565. Epub 2019 May 27.

Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, China.

Anesthetics could induce cognitive dysfunctions, such as Alzheimer's disease in humans or mice. However, the precise molecular mechanism is unclear. Sevoflurane is a common anesthetic widely used in clinical practice. Here, we demonstrated the induction of cognitive dysfunction induced by Sev in mice to corroborate the signaling pathway and the differentially expressed genes (DEGs) followed by analyzing their functions. The cognitive function of mice was measured by the Morris water maze test. Transcriptomic data were annotated with Illumina HiSeq. 2000. Further, the changes in related proteins or genes were analyzed by western blotting and real-time quantitative polymerase chain reaction. Our results showed that Sev could cause a decline in cognitive competence in mice. The transcriptomic data indicated that adding up to 566 genes were upregulated and 1073 genes were downregulated. The genes of Plin4, Lcn2, Lrg1, Foxf1, and Ctla2a were significantly upregulated, while the genes of Arc, Npas4, Egr2, Hes5, and Cdh9 were downregulated dramatically. The Gene Ontology term with the highest enrichment of DEGs are involved in the regulation of cellular and macromolecule metabolism and cation and nucleic acid binding, respectively. The Kyoto encyclopedia of genes and genomes analysis indicated that the mitogen-activated protein kinases (MAPK) pathway was one of the most important metabolic pathways. In addition, the metabolic pathways related to cognitive function, such as the nervous system and neurodegenerative disease showed significant changes. Furthermore, we found that p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase of the MAPK signaling pathway played important roles in this process. In conclusion, these results provide the first important clues for identifying the DEGs and signaling pathways in the hippocampus due to a Sev-induced cognitive deficiency in mice.
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http://dx.doi.org/10.1002/jcb.29020DOI Listing
October 2019

Joint clock recovery and feed-forward equalization for PAM4 transmission.

Opt Express 2019 Apr;27(8):11385-11395

With the rapid development of cloud services, data-center applications and the Internet of Things, short-reach communications have attracted much more attention in recent years. 4-level pulse amplitude modulation (PAM4) is a promising modulation format to provide both high data rate and relatively low cost for short-reach optical interconnects. In this paper, a joint clock recovery and feed-forward equalization algorithm (CR-FFE) is proposed to simultaneously eliminate the inter-symbol interference (ISI) and track large sampling clock offset (SCO) in PAM4 transmission. The algorithm estimates timing error according to the difference between two tap coefficients of fractionally spaced equalizers, thus solving the problem of incompatible prerequisites between clock recovery and channel equalization. A 10GHz directly modulated laser (DML) based 50-Gbit/s PAM4 transmission experiment is implemented to investigate the performance of the proposed algorithm. Experimental results show that the proposed CR-FFE algorithm can resist SCO up to 1000 ppm after 40 km standard single-mode fiber (SSMF) transmission under the 2x10 SD-FEC BER threshold, which is dramatically improved comparing with that of 20 ppm in traditional CR cascaded by FFE algorithm.
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http://dx.doi.org/10.1364/OE.27.011385DOI Listing
April 2019

Generation of LP/LP modes with tunable mode lobe orientation controlled by polarization states.

Opt Express 2019 Apr;27(9):13150-13159

We propose and experimentally demonstrate a novel scheme to generate LP/LP modes with tunable mode lobe orientation (MLO). Wherein, the MLOs have an excellent linear relationship with the linearly-polarized states of input fundamental modes. The proposed scheme is composed of a polarization controller (PC), a mode converter, a mode and polarization controller (PMC) which is twined with the few mode fiber (FMF) and a polarizer. Experimental results show that the deviations of MLOs between generated LP/LP modes and simulated ones are less than 3.5 and 8 degrees over C band. Since polarization control up to nanosecond scale is available with GaAs or lithium based electro-optic modulator, the proposed scheme could enable nanosecond time scale MLO control, which would be immensely useful for optical trapping, fiber sensors and optical communications.
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http://dx.doi.org/10.1364/OE.27.013150DOI Listing
April 2019

Crystallographically textured SnSe nanomaterials produced from the liquid phase sintering of nanocrystals.

Dalton Trans 2019 Mar;48(11):3641-3647

Catalonia Energy Research Institute - IREC, Sant Adria de Besòs, 08930 Barcelona, Spain.

We report the thermoelectric performance of p-type nanocrystalline SnSe obtained from the liquid phase sintering of blends of SnSe nanocrystals and Te nanorods. A cycled hot press procedure at a temperature above the Te melting point promoted the formation of crystallographically textured SnSe nanomaterials with relative densities up to 93%. After consolidation, part of this Te was found within the SnSe lattice and part remained as elemental Te between the SnSe grains. The presence of Te during the SnSe consolidation resulted in SnSe nanomaterials with higher electrical conductivities and lower Seebeck coefficients and thermal conductivities. By adjusting the amount of Te, thermoelectric figures of merit (ZT) up to 1.4 at 790 K were measured in the direction of the uniaxial pressure, coinciding with the preferential a crystallographic axis. While this value matches the highest ZT value reported at this temperature for SnSe in the [100] crystal direction, the ZT values of the consolidated SnSe along the bc plane were relatively lower due to moderately low thermal conductivities in this plane.
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http://dx.doi.org/10.1039/c8dt04414gDOI Listing
March 2019
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