Publications by authors named "Yong Zhou"

1,584 Publications

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Lipidomic Alterations and PPAR Activation Induced by Resveratrol Lead to Reduction in Lesion Size in Endometriosis Models.

Oxid Med Cell Longev 2021 11;2021:9979953. Epub 2021 Sep 11.

The Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China.

Endometriosis is an estrogen-dependent chronic inflammatory disease that affects approximately 10% of women of reproductive age and up to 50% of women with infertility. The heterogeneity of the disease makes accurate diagnosis and treatment a clinical challenge. In this study, we generated two models of endometriosis: the first in rats and the second using human ectopic endometrial stromal cells (HEcESCs) derived from the lesion tissues of endometriosis patients. We then applied resveratrol to assess its therapeutic potential. Resveratrol intervention had significant efficacy to attenuate lesion size and to rectify aberrant lipid profiles of model rats. Lipidomic analysis revealed significant lipidomic alterations, including notable increases of sphingolipids and decreases of both glycerolipids and most phospholipids. Upon resveratrol application, both proliferation capacity and invasiveness parameters decreased, and the early apoptosis proportion increased for HEcESCs. The activation of PPAR was also noted as a factor potentially contributing to recovery from endometriosis in both models. Our study provides valuable insight into the mechanisms of resveratrol in endometriosis and therefore strengthens the potential for optimizing resveratrol treatment for this disease.
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http://dx.doi.org/10.1155/2021/9979953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452402PMC
September 2021

Increased Risk of Lymphoma in Men or the Elderly Infected with Tuberculosis.

Mediterr J Hematol Infect Dis 2021 1;13(1):e2021053. Epub 2021 Sep 1.

Department of Lymphoma, Xinjiang Medical University Cancer Hospital, Xinjiang, China.

Purpose: To identify factors associated with lymphoma in patients with prior infection.

Methods: A retrospective case-control analysis was performed in a highly tuberculosis (TB)-endemic area. Patients with a history of TB before the diagnosis of lymphoma were retrospectively identified. Inpatients with lymphoma (n=1,057) and pathologically confirmed benign diseases (n=12,916) were consecutively enrolled at Xinjiang Medical University Cancer Hospital between January 2016 and December 2019.

Results: The proportion of TB infection in patients with lymphoma (n=148, 14.0%) was significantly higher than that in the control (benign diseases) group (n=175, 1.4%) (p<0.0001). The frequencies of TB infection in patients with Hodgkin lymphoma, B-cell non-Hodgkin lymphoma (NHL), and T/NK-cell NHL were 13.6%, 14.6%, and 11.9%, respectively. Relatively high proportions of TB infection were found in patients with chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL), marginal zone B-cell lymphoma (MZBL), and diffuse large B-cell lymphoma (DLBCL), at 20.6%, 18.6% and 15.3%, respectively, compared to other subtypes of B-cell NHL. For T/NK-cell NHL, the proportions of TB infection in patients with peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS), and anaplastic large cell lymphoma (ALCL) were 18.2% and 20%, respectively. The multivariate analysis revealed that male sex was an adverse risk factor for lymphoma after tubercular infection. In addition, male sex and older age (>60 years) were associated with B-cell NHL.

Conclusion: A high proportion of TB infection was found in patients with lymphoma. In TB-infected patients, older age and male sex were associated with susceptibility to lymphoma, suggesting that screening programmes might be useful for the early detection of lymphoma. Keywords Lymphoma; tuberculosis; Burkitt's lymphoma; diffuse large B lymphoma; Hodgkin's disease.
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http://dx.doi.org/10.4084/MJHID.2021.053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425346PMC
September 2021

CD226 Is Required to Maintain Megakaryocytes/Platelets Homeostasis in the Treatment of Knee Osteoarthritis With Platelet-Rich Plasma in Mice.

Front Pharmacol 2021 30;12:732453. Epub 2021 Aug 30.

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China.

Platelet-rich plasma (PRP) is a platelet-based application used to treat osteoarthritis (OA) clinically. The co-stimulatory molecule CD226 is expressed in T cells, NK cells, and also platelets. However, exact effects of CD226 on platelets and whether its expression level influences PRP efficacy are largely unknown. Here, CD226PF4-Cre mice were obtained from mating CD226 mice with PF4-Cre mice. Blood samples and washed platelets were collected from the mice eyeballs to undergo routine blood tests and transmission electron microscopy. Differentially expressed proteins were detected by iTRAQ-based proteomics analysis. Animal OA models were established through surgical destabilization of the medial meniscus (DMM) for C57BL/6 wildtype mice, followed by PRP injection to evaluate the effects of platelet CD226 on PRP efficacy. The results showed that deletion of platelet CD226 increased the number of megakaryocytes (MKs) in bone marrow (BM) but reduced MKs in spleen, combined with significantly decreased platelet amounts, α-granule secretion, and reduced immature platelets; indicating that absence of platelet CD226 may disrupt MK/platelet homeostasis and arrested platelet release from MKs. Sequencing analysis showed abnormal ribosomal functions and much downregulated proteins in the absence of platelet CD226. Autophagy-related proteins were also reduced in the CD226-absent MKs/platelets. Moreover, deletion of platelet CD226 diminished the protective effects of PRP on DMM-induced cartilage lesions in mice, and PDGF restored it. Therefore, deficiency of platelet CD226 inhibited platelet maturation, secretion, and normal ribosomal functions, which may lead to depressed PRP efficacy on OA, suggesting that CD226 is required to regulate platelet growth, functions, and its application.
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http://dx.doi.org/10.3389/fphar.2021.732453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436152PMC
August 2021

Baseline Gut Metagenomic Functional Gene Signature Associated with Variable Weight Loss Responses following a Healthy Lifestyle Intervention in Humans.

mSystems 2021 Sep 14:e0096421. Epub 2021 Sep 14.

Institute for Systems Biologygrid.64212.33, Seattle, Washington, USA.

Recent human feeding studies have shown how the baseline taxonomic composition of the gut microbiome can determine responses to weight loss interventions. However, the functional determinants underlying this phenomenon remain unclear. We report a weight loss response analysis on a cohort of 105 individuals selected from a larger population enrolled in a commercial wellness program, which included healthy lifestyle coaching. Each individual in the cohort had baseline blood metabolomics, blood proteomics, clinical labs, dietary questionnaires, stool 16S rRNA gene sequencing data, and follow-up data on weight change. We generated additional targeted proteomics data on obesity-associated proteins in blood before and after intervention, along with baseline stool metagenomic data, for a subset of 25 individuals who showed the most extreme weight change phenotypes. We built regression models to identify baseline blood, stool, and dietary features associated with weight loss, independent of age, sex, and baseline body mass index (BMI). Many features were independently associated with baseline BMI, but few were independently associated with weight loss. Baseline diet was not associated with weight loss, and only one blood analyte was associated with changes in weight. However, 31 baseline stool metagenomic functional features, including complex polysaccharide and protein degradation genes, stress-response genes, respiration-related genes, and cell wall synthesis genes, along with gut bacterial replication rates, were associated with weight loss responses after controlling for age, sex, and baseline BMI. Together, these results provide a set of compelling hypotheses for how commensal gut microbiota influence weight loss outcomes in humans. Recent human feeding studies have shown how the baseline taxonomic composition of the gut microbiome can determine responses to dietary interventions, but the exact functional determinants underlying this phenomenon remain unclear. In this study, we set out to better understand interactions between baseline BMI, metabolic health, diet, gut microbiome functional profiles, and subsequent weight changes in a human cohort that underwent a healthy lifestyle intervention. Overall, our results suggest that the microbiota may influence host weight loss responses through variable bacterial growth rates, dietary energy harvest efficiency, and immunomodulation.
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http://dx.doi.org/10.1128/mSystems.00964-21DOI Listing
September 2021

Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome.

Immun Inflamm Dis 2021 Sep 13. Epub 2021 Sep 13.

Experimental Center of Clinical Research, Scientific Research Department, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China.

Introduction: To compare the saliva proteomes of experimental Sjögren's syndrome (ESS) model mice and healthy controls to identify potential diagnostic biomarkers for primary Sjögren's syndrome (pSS).

Methods: Proteins were extracted from the saliva of three ESS and three normal control mice using the data-independent acquisition technique. R language was used to identify the differentially expressed proteins (DEPs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to functionally annotate the DEPs. The protein-protein interaction (PPI) network was constructed and the core proteins were identified with the STRING website and Cytoscape software. The concentrations of Serpin family G member 1 (SERPING1), C3, complement factor H (CFH), fibrinogen alpha (FGA), and fibrinogen gamma (FGG) in saliva were determined by ELISA.

Results: A total of 1722 DEPs were identified in the saliva of the ESS mice relative to the controls, of which 50 showed significantly different expression levels between the two groups. SERPING1, C3, CFH, FGA, and FGG were significantly downregulated, and keratin 4 (Krt4) and transglutaminase 3 (TGM3) were upregulated in the saliva of ESS mice. The PPI network showed that SERPING1, C3, FGG, FGA, TGM3, and hemopexin (HPX) were the core proteins. ELISA results showed that the expression of C3, CFH, FGA, and SERPING1 were significantly downregulated in the saliva of ESS mice. However, the expression of FGG was a little downregulated but with no significant difference. SERPING1, FGG, and FGA may downregulate the complement C3 by inhibiting immune complement system, thereby promoting pSS progression.

Conclusions: The salivary proteome of ESS mice was markedly different from that of healthy controls, suggesting that salivary proteomics is a promising noninvasive diagnostic tool for pSS. SERPING1, C3, CFH, FGA, and FGG are potential biomarkers of pSS.
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http://dx.doi.org/10.1002/iid3.529DOI Listing
September 2021

LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1.

Cancer Cell Int 2021 Sep 8;21(1):478. Epub 2021 Sep 8.

Hunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, People's Republic of China.

Background: The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3's levels and role in esophageal squamous cell carcinoma (ESCC) has not been elucidated.

Methods: GACAT3 amounts were investigated in ESCC tissues and cell lines by qPCR. Its biological functions were examined by CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, transwell assay, and xenograft model establishment. The relationship between GACAT3 and miR-149 was assessed by dual-luciferase reporter assay.

Results: GACAT3 amounts were elevated in ESCC tissue and cell specimens. Functional studies showed that GACAT3 silencing reduced the proliferation, migration and invasion of cultured ESCC cells, and decreased tumor growth in mice. Furthermore, GACAT could directly interact with miR-149. In addition, colony formation and invasion assays verified that GACAT3 promotes ESCC tumor progression through miR-149. Moreover, GACAT3 acted as a competing endogenous RNA (ceRNA) to modulate FOXM1 expression.

Conclusions: These findings indicate that GACAT3 functions as an oncogene by acting as a ceRNA for miR-149 to modulate FOXM1 expression in ESCC, suggesting that GACAT3 might constitute a therapeutic target in ESCC.
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http://dx.doi.org/10.1186/s12935-021-02192-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424903PMC
September 2021

Hollow CuO nanospheres loaded with MoS/reduced graphene oxide nanosheets for ppb-level NO detection at room temperature.

J Hazard Mater 2021 Aug 26;416:126218. Epub 2021 May 26.

Key Laboratory of Optoelectronic Technology and Systems (Ministry of Education), Chongqing University, Chongqing 400044, China. Electronic address:

Low energy consumption, high sensing response and high selectivity are the important indexes of metal oxide semiconductor (MOS) gas sensors applied in many application fields. However, the high working temperature and poor selectivity of MOS sensors severely restrict their scope of application in the Internet of Things (IoT). Herein, ternary MoS-rGO-CuO (MG-Cu) composites with boosting ppb-level NO sensing characteristics are synthesized by combining hydrothermal method and soft-template method. The optimal proportion of MoS, rGO and CuO is systematically explored. The SEM and TEM analyses confirm the hollow CuO is anchored on the surface of MG. The gas sensing tests illustrate that optimum composite sensor exhibits highest response to 500 ppb NO at room temperature, which is 11 and 5 times higher compared to pure MoS and binary MG15, respectively. Besides, it displays excellent selectivity and superior stability. The synergy of shell-structure with abundant mesoporous, heterojunction construction and enhanced conductivity lead to the enhanced sensing performance of ternary sensor.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126218DOI Listing
August 2021

Integration of Molecular Inflammatory Interactome Analyses Reveals Dynamics of Circulating Cytokines and Extracellular Vesicle Long Non-Coding RNAs and mRNAs in Heroin Addicts During Acute and Protracted Withdrawal.

Front Immunol 2021 19;12:730300. Epub 2021 Aug 19.

National Health Commission (NHC) Key Laboratory of Drug Addiction Medicine (Kunming Medical University), The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Heroin addiction and withdrawal influence multiple physiological functions, including immune responses, but the mechanism remains largely elusive. The objective of this study was to investigate the molecular inflammatory interactome, particularly the cytokines and transcriptome regulatory network in heroin addicts undergoing withdrawal, compared to healthy controls (HCs). Twenty-seven cytokines were simultaneously assessed in 41 heroin addicts, including 20 at the acute withdrawal (AW) stage and 21 at the protracted withdrawal (PW) stage, and 38 age- and gender-matched HCs. Disturbed T-helper(T)1/T2, T1/T17, and T2/T17 balances, characterized by reduced interleukin (IL)-2, elevated IL-4, IL-10, and IL-17A, but normal TNF-α, were present in the AW subjects. These imbalances were mostly restored to the baseline at the PW stage. However, the cytokines TNF-α, IL-2, IL-7, IL-10, and IL-17A remained dysregulated. This study also profiled exosomal long non-coding RNA (lncRNA) and mRNA in the plasma of heroin addicts, constructed co-expression gene regulation networks, and identified lncRNA-mRNA-pathway pairs specifically associated with alterations in cytokine profiles and T1/T2/T17 imbalances. Altogether, a large amount of cytokine and exosomal lncRNA/mRNA expression profiling data relating to heroin withdrawal was obtained, providing a useful experimental and theoretical basis for further understanding of the pathogenic mechanisms of withdrawal symptoms in heroin addicts.
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http://dx.doi.org/10.3389/fimmu.2021.730300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416766PMC
August 2021

Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway.

Biol Pharm Bull 2021 Sep 1. Epub 2021 Sep 1.

Department of Nutrition and Food Hygiene, School of Public Health and Management, Chongqing Medical University.

Ampelopsin, a flavonoid with a wide variety of biological activities, has been proposed to be a potent antitumor agent. However, the mechanism by which Ampelopsin shows anti-breast cancer activity remains unclear. Therefore, this study will explore the mechanism of Ampelopsin's anti-breast cancer activity by culturing MDA-MB-231 and MCF-7 breast cancer cells. CCK-8 method and plate cloning method were used to detect the proliferation inhibition of breast cancer cells. Fluorescence microscopy was used to detect mitochondrial membrane potential (MMP). DCFH-DA method was used to determine the content of intracellular reactive oxygen species (ROS). Hoechst 33258 staining was used to detect the apoptotic morphological changes. Transmission electron microscope was used to observe the mitochondrial structure. Western Blot was used to detect the protein expression of Bax and Bcl-2. The results showed that Ampelopsin could significantly inhibit the proliferation of breast cancer cells, and promote cells apoptosis. In addition, the occurrence of apoptosis in breast cancer cells was associated with mitochondrial dysfunction, including the loss of mitochondrial membrane potential, the production of large amounts of reactive oxygen species, and the up-regulation of Bax/Bcl-2 expression. In conclusion, Ampelopsin-induced mitochondria damage leads to loss of mitochondria membrane potential, overproduction of ROS and activation of Bax, increasing mitochondria membrane permeability and ultimately inducing breast cell apoptosis. These findings provided a new perspective on the role of Ampelopsin in breast cancer prevention and treatment.
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http://dx.doi.org/10.1248/bpb.b21-00470DOI Listing
September 2021

Polarization conversion in anisotropic dielectric metasurfaces originating from bound states in the continuum.

Opt Lett 2021 Sep;46(17):4120-4123

We use the semianalytical Cartesian multipole method to investigate the light transmission and reflection spectra of anisotropic dielectric metasurfaces, and extend the multipole decompositions method to account for cross-polarization conversion effects. We observe sharp high- resonances arising from a distortion of symmetry-protected bound states in the continuum in asymmetric dielectric metasurfaces, i.e., quasibound states in the continuum. In addition, by further introducing in-plane symmetric breaking perturbation, the polarization conversions of linearly polarized light can be achieved through quasibound states in the continuum. With the aid of temporal coupled-mode theory, we can obtain the limit of cross-conversion under single high- resonance, i.e., 0.25. Our work will help to design dielectric metasurfaces to control the polarization states of light.
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http://dx.doi.org/10.1364/OL.431047DOI Listing
September 2021

T cell exhaustion is associated with the risk of papillary thyroid carcinoma and can be a predictive and sensitive biomarker for diagnosis.

Diagn Pathol 2021 Aug 31;16(1):84. Epub 2021 Aug 31.

Precision Medicine Center, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, Zhejiang, People's Republic of China.

Background: The incidence of papillary thyroid carcinoma (PTC) has been steadily increasing over the past decades. Hashimoto's thyroiditis (HT) is the most common autoimmune disease, and is related to the pathogenesis of PTC. Programmed death-1 (PD-1) is currently used for the treatment of PTC, but there are very few studies on the clinical value of PD-1 in the diagnosis and targeted therapy of PTC.

Methods: The expression of T, B, NK cells and PD-1 in the peripheral blood of 132 patients with PTC (PTC group), 48 patients with nodular goiter (NG group) and 63 healthy subjects (HP group) were detected by flow cytometry. The expression of plasma T3, T4, FT3, FT4, TSH, TGAb and TPO was detected by chemiluminescence immunoassay. Among 132 PTC, 49 PTC&HT and 83 PTC&noHT were included. Among 48 NG, 10 NG&HT and 38 NG&noHT were included. The expressions of programmed death- ligand1(PD-L1) in tumor tissues of PTC group and thyroid tissues of NG group, PD-1 and CD3 in tumor infiltration lymphocyte (TIL) were detected by immunohistochemistry.

Results: The expression of FT3, TGAb, CD3PD-1, CD3CD4PD-1 and CD3CD8PD-1 in PTC and NG was significantly higher than that in the HP group. Moreover, CD3PD-1, CD3CD4PD-1 and CD3CD8PD-1 expression had significant differences between the PTC group and the NG group. In addition, the expression of TGAb, TPO, CD3PD-1, CD3CD4PD-1 and CD3CD8PD-1 in PTC&HT group was significantly higher than that in the PTC&noHT group. While, the expression of B cells, CD3PD-1, CD3CD4PD-1 and CD3CD8PD-1 in PTC&HT group was higher than that in NG&HT group. PD-1 showed a significant correlation with PTC lymph node metastasis. CD3PD-1 and CD3CD4PD-1 was higher in N1 stage than in N0 stage. Immunohistochemical results showed that the expression of PD-1, CD3 and PD-L1 in PTC was significantly higher than that in NG.

Conclusions: T cell exhaustion might act as a biomarker for the differential diagnosis of PTC and NG. Patients with PTC&HT have obvious T cell exhaustion and increased expression of PD-1, PD-L1.Targeting the PD-1/PD-L1 pathway could be a new approach to prevent malignant transformation from HT to PTC&HT in the future.
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http://dx.doi.org/10.1186/s13000-021-01139-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408957PMC
August 2021

Designing Ecological Security Patterns Based on the Framework of Ecological Quality and Ecological Sensitivity: A Case Study of Jianghan Plain, China.

Int J Environ Res Public Health 2021 08 8;18(16). Epub 2021 Aug 8.

The College of Urban & Environmental Sciences, Central China Normal University, Wuhan 430079, China.

Researchers and managers of natural resource conservation have increasingly emphasized the importance of maintaining a connected network of important ecological patches to mitigate landscape fragmentation, reduce the decline of biodiversity, and sustain ecological services. This research aimed to guide landscape management and decision-making by developing an evaluation framework to construct ecological security patterns. Taking the Jianghan Plain as the study area, we identified key ecological sources by overlaying the spatial patterns of ecological quality (biodiversity, carbon storage, and water yield) and ecological sensitivity (habitat sensitivity, soil erosion sensitivity, and water sensitivity) using the Integrated Valuation of Environmental Services and Tradeoffs (InVEST) model and the Chinese Soil Loss Equation Function. Ecological corridors were obtained by the least-cost path analysis method and circuit theory. A total of 48 ecological sources (3812.95 km), primarily consisting of water area, forestland, and cropland, were identified. Ninety-one ecological corridors were derived, with a total length of 2036.28 km. Forty barriers and 40 pinch points with the highest improvement coefficient scores or priority scores were selected. There were 11 priority corridors with very high levels of connectivity improvement potential and conservation priority, occupying 16.15% of the total length of corridors. The overall potential for ecological connectivity is high on the Jianghan Plain. Our framework offers a valuable reference for constructing ecological security patterns and identifying sites for ecological restoration at the regional scale.
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http://dx.doi.org/10.3390/ijerph18168383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392237PMC
August 2021

Management of heterotopic cesarean scar pregnancy with preservation of intrauterine pregnancy: A case report.

World J Clin Cases 2021 Aug;9(22):6428-6434

Department of Gynecology, Women's Hospital School of Medicine, Zhejiang University, Hangzhou 310006, Zhejiang Province, China.

Background: Heterotopic cesarean scar pregnancy (HCSP) is very rare and has a high risk of massive uterine bleeding. Preservation of concurrent intrauterine pregnancy (IUP) is one of the great challenges in the management of HCSP. No universal treatment protocol has been established when IUP is desired to be preserved.

Case Summary: We report a case of HCSP at 8 wk gestation in a 34-year-old woman with stable hemodynamics. A two-step intervention was applied. Selective embryo aspiration was performed first, and surgical removal of ectopic gestational tissue by suction and curettage was performed 2 d later. Both steps were performed under ultrasound guidance. The patient had an uneventful course, and a healthy baby was delivered at 34 wk gestation.

Conclusion: Selective embryo aspiration followed by suction and curettage was successful in the preservation of IUP in the management of HCSP. This approach is an alternative option for HCSP in the first trimester when the IUP is desired to be preserved.
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http://dx.doi.org/10.12998/wjcc.v9.i22.6428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362565PMC
August 2021

Allicin inclusions with α-cyclodextrin effectively masking its odor: Preparation, characterization, and olfactory and gustatory evaluation.

J Food Sci 2021 Sep 18;86(9):4026-4036. Epub 2021 Aug 18.

Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Allicin, a chemical found in functional foods, has a variety of beneficial bioactivities but the unpleasent odor and unstability hinder its applications. Isolating products from cyclodextrin (CD) complexation, using β-CD and its derivatives, is usually a time and energy-consuming process. Herein, a high-efficiency and eco-friendly preparation method of an inclusion ([email protected]α-CD) formed by allicin and α-CD was designed, which turned liquid allicin into crystal particles with high-speed stirring (10,000 r/min) at 25°C for 10 min in water. In vivo and in vitro masking evaluations showed that the inclusion particles could decrease the unpleasant odor of allicin. Molecular docking and experimental characterization results illustrated that the main reason of odor masking was due to the disulfide and thiocarbonyl groups of allicin being partially encapsulated by the cavity of α-CD. Compared with the physical mixture, the stability of allicin in [email protected]α-CD at 60°C for 10 days was 33-fold improved. Overall, this efficient strategy of inclusion provided a promising approach for the industrialization of allicin-related formulations. PRACTICAL APPLICATION: In this study, an environmentally friendly method of α-CD inclusion without the use of organic reagents was designed to solidify and stabilize allicin, which effectively masked the unpleasant odor and taste of allicin. It has contributed greatly to improving the compliance of consumers and provided a new and effective approach to broaden the application of allicin.
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http://dx.doi.org/10.1111/1750-3841.15882DOI Listing
September 2021

Targeted Downregulation of Increases Chemosensitivity of Lung Cancer Cells.

Genet Test Mol Biomarkers 2021 Aug;25(8):540-545

School of Public Health, Xiang Nan University, Chenzhou, China.

To investigate the expression of B cell lymphoma-2 () in lung cancer cells and the effect of the / axis on the chemosensitivity of lung cancer. Real-time quantitative PCR and western blotting were used to detect the expression of in human embryonic lung fibroblasts and lung cancer cells. The effects of siRNA directed against , in lung cancer tissue samples was detected by immunohistochemistry; these results were used to develop prognostic models. Bioinformatic analyses, dual luciferase reporter gene technology, and western blotting technology were used to explore the targeted regulation of on bcl-2. The effect of on the chemosensitivity of lung cancer cells was measured using the MTT assay. Compared with human embryonic lung fibroblasts, was highly expressed in the lung cancer cells, especially in H460 cells. After silencing with siRNA, the sensitivity of the cells to cisplatin (CDDP) increased. Immunohistochemical results and prognostic analysis revealed that high expression in lung cancer tissues was negatively correlated with prognosis of lung cancer patients; A dual luciferase reporter assay combined with western blotting confirmed that can bind to the 3' UTR region and regulate its expression. Overexpression of in lung cancer cells (H460 and A549) increased the sensitivity of these cells to CDDP. is upregulated in lung cancer cells, which is negatively correlated with the patient prognosis. affects the chemosensitivity of lung cancer cells by targeting . These data should provide a theoretical basis for refining the molecular mechanisms of chemoresistance in lung cancer.
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http://dx.doi.org/10.1089/gtmb.2021.0009DOI Listing
August 2021

SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas.

J Cancer 2021 13;12(18):5439-5453. Epub 2021 Jul 13.

Department of Epidemiology and Health Statistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.

Papillary thyroid carcinoma (PTC) is one of the fastest growing endocrine system malignant carcinomas detected over the past decade. Unfortunately, more than 25% of PTC patients are characterized by their aggressiveness and subsequent metastasis; these characteristics usually indicate poor prognosis. Recently, increasing evidence has suggested that solute carrier (SLC) transporters may play a pivotal role in the initiation, invasion and metastasis of human carcinoma. However, the expression and clinicopathological significance of SLC transporters in patients with PTC remains undetermined. In this study, we aimed to elucidate how the differential expression of SLC transporters affects clinicopathological features, as well as determine the possible regulatory signaling pathways involved. Three differentially expressed SLC transporters were screened from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database using a bioinformatics approach. The results indicated that high SLC34A2 and low SLC4A4 protein expression exhibited a higher percentage of capsular invasion and extra-thyroid metastasis in patients. Logistic regression analysis showed that high SLC34A2 expression in tumors was identified as an independent risk factor for capsular invasion [odds ratio (OR)=11.400, 95% confidence interval (CI)=1.733-74.995, P=0.011] and extra-thyroid metastasis (OR=4.920, 95%CI=1.234-19.623, P=0.024), while low SLC4A4 expression in tumors was only identified as independent risk factors for extra-thyroid metastasis (OR=8.568, 95%CI =1.186-61.906, P=0.033). Specifically, for tumors with capsular invasion and extra-thyroid metastasis, the protein expression staining of SLC34A2 was markedly enhanced in the cytoplasm of follicular epithelial cells, contrastingly, SLC4A4 expression was notably weakened in the cytomembrane and nucleus. Intriguingly, both high SLC34A2 and low SLC4A4 protein expression were significantly linked to a high urinary iodine concentration in patients with PTC. Mechanistically, compared with adjacent normal thyroids, p-ERK was significantly up-regulated by 17.8% in the invading tumor; p-ERK, p-JNK, and p-P38 were markedly up-regulated by 29.2%, 67.1%, and 38.9% for metastatic tumors, respectively. Importantly, SLC4A4 negatively correlated with p-JNK (r=-0.696, P= 0.004) and p-P38 (r=-0.534, P=0.049). In conclusion, we suggest that up-regulated SLC34A2 (mainly in the cytoplasm) and down-regulated SLC4A4 (mainly in the cytomembrane and nucleus), which might be attributed to excess iodine intake, were closely linked to extra-thyroid metastasis in PTCs. Furthermore, this effect of SLC4A4 may be through the activation of JNK/P38 MAPK signaling pathway. Future and gain- or loss-of-function experiments are needed to verify these findings and further elucidate the deeper molecular mechanisms.
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http://dx.doi.org/10.7150/jca.56730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364650PMC
July 2021

Hollow InVO Nanocuboid Assemblies toward Promoting Photocatalytic N Conversion Performance.

Adv Mater 2021 Aug 15:e2006780. Epub 2021 Aug 15.

Key Laboratory of Modern Acoustics (MOE), Institute of Acoustics, Jiangsu Key Laboratory for Nano Technology, Ecomaterials and Renewable Energy Research Center (ERERC), School of Physics, National Laboratory of Solid State Microstructures, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu, 210093, P. R. China.

The unique InVO mesocrystal superstructure, particularly with cubical skeleton and hollow interior, which consists of numerous nanocube building blocks, closely stacking by stacking, aligning by aligning, and sharing the same crystallographic orientations, is successfully fabricated. The synergy of a reaction-limited aggregation and an Ostwald ripening process is reasonably proposed for the growth of this unique superstructure. Both single-particle surface photovoltage and confocal fluorescence spectroscopy measurements demonstrate that the long-range ordered mesocrystal superstructures can significantly retard the recombination of electron-hole pairs through the creation of a new pathway for anisotropic electron flow along the inter-nanocubes. This promising charge mobility feature of the superstructure greatly contributes to the pronounced photocatalytic performance of the InVO mesocrystal toward fixation of N into NH with the quantum yield of 0.50% at wavelength of 385 nm.
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http://dx.doi.org/10.1002/adma.202006780DOI Listing
August 2021

Dissipation and dietary risk assessment of carbendazim and epoxiconazole in citrus fruits in China.

J Sci Food Agric 2021 Aug 10. Epub 2021 Aug 10.

College of Plant Protection, Hunan Agricultural University, Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Changsha, China.

Background: Carbendazim and epoxiconazole are widely applied to control anthracnose and sand bark fungal diseases in citrus. The residues of these two fungicides in citrus and their potential risk to consumers have generated much public concern. We therefore sought to investigate the dissipation, residue, and dietary risk assessment of carbendazim and epoxiconazole in citrus.

Results: The dissipation kinetics and residue levels of carbendazim and epoxiconazole in citrus under field conditions were measured using dispersive solid-phase extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry. The citrus samples were extracted with acetonitrile and purified by primary secondary amine sorbent. The mean recoveries of carbendazim and epoxiconazole ranged from 86.2 to 105.6% and relative standard deviations were ≤9.8%. The half-lives of carbendazim and epoxiconazole in whole citrus ranged from 2.0 to 18.0 days. Hazard quotient (HQ) and risk quotient (RQ) models were applied to whole citrus for dietary exposure risk assessment based on the terminal residue test. Hazard quotients ranged from 0.066 to 0.134% and RQs from 18.48 to 82.12%.

Conclusion: Carbendazim and epoxiconazole in citrus degraded rapidly following first-order kinetics models. The dietary risk of exposure to both carbendazim and epoxiconazole through citrus, based on HQ and RQ, was acceptable for human consumption. This study indicates scientifically validated maximum residue limits in citrus, which are currently lacking for epoxiconazole in China. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11474DOI Listing
August 2021

Woody encroachment happens via intensification, not extensification, of species ranges in an African savanna.

Ecol Appl 2021 Aug 9:e02437. Epub 2021 Aug 9.

Department of Ecology & Evolutionary Biology, Yale University, New Haven, Connecticut, 06511, USA.

Widespread woody encroachment is a prominent concern for savanna systems as it is often accompanied by losses in productivity and biodiversity. Extensive ecosystem-level work has advanced our understanding of its causes and consequences. However, there is still debate over whether local management can override regional and global drivers of woody encroachment, and it remains largely unknown how encroachment influences woody community assemblages. Here, we examined species-level changes in woody plant distributions and size structure from the late 1980s to the late 2000s based on spatially intensive ground-based surveys across Kruger National Park, South Africa. This study region spans broad gradients in rainfall, soil texture, fire frequency, elephant density, and other topographic variables. Species-level changes in frequency of occurrence and size class proportion reflected widespread woody encroachment primarily by Dichrostachys cinerea and Combretum apiculatum, and a loss of large trees mostly of Sclerocarya birrea and Acacia nigrescens. Environmental variables determining woody species distributions across Kruger varied among species but did not change substantially between two sampling times, indicating that woody encroachers were thickening within their existing ranges. Overall, more areas across Kruger were found to have an increased number of common woody species through time, which indicated an increase in stem density. These areas were generally associated with decreasing fire frequency and rainfall but increasing elephant density. Our results suggest that woody encroachment is a widespread but highly variable trend across landscapes in Kruger National Park and potentially reflects an erosion of local heterogeneity in woody community assemblages. Many savanna managers, including in Kruger, aim to manage for heterogeneity in order to promote biodiversity, where homogenization of vegetation structure counters this specific goal. Increasing fire frequency has some potential as a local intervention. However, many common species increased in commonness even under near-constant disturbance conditions, which likely limits the potential for managing woody encroachment in the face of drivers beyond the scope of local control. Regular field sampling coupled with targeted fire management will enable more accurate monitoring of the rate of encroachment intensification.
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http://dx.doi.org/10.1002/eap.2437DOI Listing
August 2021

Is three-dimensional-printed custom-made ultra-short stem with a porous structure an acceptable reconstructive alternative in peri-knee metaphysis for the tumorous bone defect?

World J Surg Oncol 2021 Aug 8;19(1):235. Epub 2021 Aug 8.

Department of Orthopaedics, Orthopaedic Research Institute, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

Background: Long-lasting reconstruction after extensive resection involving peri-knee metaphysis is a challenging problem in orthopedic oncology. Various reconstruction methods have been proposed, but they are characterized by a high complication rate. The purposes of this study were to (1) assess osseointegration at the bone implant interface and correlated incidence of aseptic loosening; (2) identify complications including infection, endoprosthesis fracture, periprosthetic fracture, leg length discrepancy, and wound healing problem in this case series; and (3) evaluate the short-term function of the patient who received this personalized reconstruction system.

Methods: Between September 2016 and June 2018, our center treated 15 patients with malignancies arising in the femur or tibia shaft using endoprosthesis with a 3D-printed custom-made stem. Osseointegration and aseptic loosening were assessed with digital tomosynthesis. Complications were recorded by reviewing the patients' records. The function was evaluated with the 1993 version of the Musculoskeletal Tumor Society (MSTS-93) score at a median of 42 (range, 34 to 54) months after reconstruction.

Results: One patient who experienced early aseptic loosening was managed with immobilization and bisphosphonates infusion. All implants were well osseointegrated at the final follow-up examination. There are two periprosthetic fractures intraoperatively. The wire was applied to assist fixation, and the fracture healed at the latest follow-up. Two patients experienced significant leg length discrepancies. The median MSTS-93 score was 26 (range, 23 to 30).

Conclusions: A 3D-printed custom-made ultra-short stem with a porous structure provides acceptable early outcomes in patients who received peri-knee metaphyseal reconstruction. With detailed preoperative design and precise intraoperative techniques, the reasonable initial stability benefits osseointegration to osteoconductive porous titanium, and therefore ensures short- and possibly long-term durability. Personalized adaptive endoprosthesis, careful intraoperative operation, and strict follow-up management enable effective prevention and treatment of complications. The functional results in our series were acceptable thanks to reliable fixation in the bone-endoprosthesis interface and an individualized rehabilitation program. These positive results indicate this device series can be a feasible alternative for critical bone defect reconstruction. Nevertheless, longer follow-up is required to determine whether this technique is superior to other forms of fixation.
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http://dx.doi.org/10.1186/s12957-021-02355-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349501PMC
August 2021

Vacancy-defect modulated pathway of photoreduction of CO on single atomically thin AgInPS sheets into olefiant gas.

Nat Commun 2021 Aug 6;12(1):4747. Epub 2021 Aug 6.

Key Laboratory of Modern Acoustics (MOE), Institute of Acoustics, School of Physics, Jiangsu Key Laboratory of Nanotechnology, Eco-materials and Renewable Energy Research Center (ERERC), National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, China.

Artificial photosynthesis, light-driving CO conversion into hydrocarbon fuels, is a promising strategy to synchronously overcome global warming and energy-supply issues. The quaternary AgInPS atomic layer with the thickness of ~ 0.70 nm were successfully synthesized through facile ultrasonic exfoliation of the corresponding bulk crystal. The sulfur defect engineering on this atomic layer through a HO etching treatment can excitingly change the CO photoreduction reaction pathway to steer dominant generation of ethene with the yield-based selectivity reaching ~73% and the electron-based selectivity as high as ~89%. Both DFT calculation and in-situ FTIR spectra demonstrate that as the introduction of S vacancies in AgInPS causes the charge accumulation on the Ag atoms near the S vacancies, the exposed Ag sites can thus effectively capture the forming *CO molecules. It makes the catalyst surface enrich with key reaction intermediates to lower the C-C binding coupling barrier, which facilitates the production of ethene.
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http://dx.doi.org/10.1038/s41467-021-25068-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346554PMC
August 2021

Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay.

Foods 2021 Jul 20;10(7). Epub 2021 Jul 20.

College of Food Science, Southwest University, Chongqing 400715, China.

Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 μg kg for SEM, respectively, with the linear range of 0.08-0.41 μg L, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation <15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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http://dx.doi.org/10.3390/foods10071668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307092PMC
July 2021

Superfast Water Transport Zwitterionic Polymeric Nanofluidic Membrane Reinforced by Metal-Organic Frameworks.

Adv Mater 2021 Sep 4;33(38):e2102292. Epub 2021 Aug 4.

Center for Membrane and Water Science and Technology, Zhejiang University of Technology, Hangzhou, 310014, China.

Nanofluidics derived from low-dimensional nanosheets and protein nanochannels are crucial for advanced catalysis, sensing, and separation. However, polymer nanofluidics is halted by complicated preparation and miniaturized sizes. This work reports the bottom-up synthesis of modular nanofluidics by confined growth of ultrathin metal-organic frameworks (MOFs) in a polymer membrane consisting of zwitterionic dopamine nanoparticles (ZNPs). The confined growth of the MOFs on the ZNPs reduces the chain entanglement between the ZNPs, leading to stiff interfacial channels enhancing the nanofluidic transport of water molecules through the membrane. As such, the water permeability and solute selectivity of [email protected] are one magnitude improved, leading to a record-high performance among all polymer nanofiltration membranes. Both the experimental work and the molecular dynamics simulations confirm that the water transport is shifted from high-friction-resistance conventional viscous flow to ultrafast nanofluidic flow as a result of rigid and continuous nanochannels in [email protected]
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http://dx.doi.org/10.1002/adma.202102292DOI Listing
September 2021

A Nomogram for Predicting Acute Respiratory Failure After Cervical Traumatic Spinal Cord Injury Based on Admission Clinical Findings.

Neurocrit Care 2021 Aug 3. Epub 2021 Aug 3.

Department of Neurosurgery, Qingdao Municipal Hospital, No. 5 Donghai Zhong Road, Qingdao, 266071, Shandong, People's Republic of China.

Objectives: Acute respiratory failure (ARF) is a common medical complication in patients with cervical traumatic spinal cord injury (TSCI). To identify independent predictors for ARF onset in patients who underwent cervical TSCI without premorbid respiratory diseases and to apply appropriate medical supports based on accurate prediction, a nomogram relating admission clinical information was developed for predicting ARF during acute care period.

Methods: We retrospectively reviewed clinical profiles of patients who suffered cervical TSCI and were emergently admitted to Qingdao Municipal Hospital from 2014 to 2020 as the training cohort. Univariate analysis was performed using admission clinical variables to estimate associated factors and a nomogram for predicting ARF occurrence was generated based on the independent predictors from multivariate logistic regression analysis. This nomogram was assessed by concordance index for discrimination and calibration curve with internal-validated bootstrap strategy. Receiver operating characteristic curve was conducted to compare the predictive accuracy between the nomogram and the traditional gold standard, which combines neuroimaging and neurological measurements by using area under the receiver operating characteristic curve (AUC). An additional 56-patient cohort from another medical center was retrospectively reviewed as the test cohort for external validation of the nomogram.

Results: 162 patients were eligible for this study and were included in the training cohort, among which 25 individuals developed ARF and were recorded to endure more complications. Despite the aggressive treatments and prolonged intensive care unit cares, 14 patients insulted with ARF died. Injury level, American Spinal Injury Association Impairment Scale (AIS) grade, admission hemoglobin (Hb), platelet to lymphocyte ratio, and neutrophil percentage to albumin ratio (NPAR) were independently associated with ARF onset. The concordance index of the nomogram incorporating these predictors was 0.933 in the training cohort and 0.955 in the test cohort, although both calibrations were good. The AUC of the nomogram was equal to concordance index, which presented better predictive accuracy compared with previous measurements using neuroimaging and AIS grade (AUC 0.933 versus 0.821, Delong's test p < 0.001). Similar significant results were also found in the test cohort (AUC 0.955 versus 0.765, Delong's test p = 0.034). In addition, this nomogram was translated to a Web-based calculator that could generate individual probability for ARF in a visualized form.

Conclusions: The nomogram incorporating the injury level, AIS grade, admission Hb, platelet to lymphocyte ratio, and NPAR is a promising model to predict ARF in patients with cervical TSCI who are absent from previous respiratory dysfunction. This nomogram can be offered to clinicians to stratify patients, strengthen evidence-based decision-making, and apply appropriate individualized treatment in the field of acute clinical care.
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http://dx.doi.org/10.1007/s12028-021-01302-4DOI Listing
August 2021

Engineering Self-Reconstruction via Flexible Components in Layered Double Hydroxides for Superior-Evolving Performance.

Small 2021 Sep 3;17(38):e2101671. Epub 2021 Aug 3.

Institute of Optoelectronics and Nanomaterials, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

Most transition metal-based catalysts for electrocatalytic oxygen evolution reaction (OER) undergo surface reconstruction to generate real active sites favorable for high OER performance. Herein, how to use self-reconstruction as an efficient strategy to develop novel and robust OER catalysts by designing pre-catalysts with flexible components susceptible to OER conditions is proposed. The NiFe-based layered double hydroxides (LDHs) intercalated with resoluble molybdate (MoO ) anions in interlayers are constructed and then demonstrated to achieve complete electrochemical self-reconstruction (ECSR) into active NiFe-oxyhydroxides (NiFeOOH) beneficial to alkaline OER. Various ex situ and in situ techniques are used to capture structural evolution process including fast dissolution of MoO and deep reconstruction to NiFeOOH upon simultaneous hydroxyl invasion and electro-oxidation. The obtained NiFeOOH exhibits an excellent OER performance with an overpotential of only 268 mV at 50 mA cm and robust durability over 45 h, much superior to NiFe-LDH and commercial IrO benchmark. This work suggests that the ECSR engineering in component-flexible precursors is a promising strategy to develop highly active OER catalysts for energy conversion.
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http://dx.doi.org/10.1002/smll.202101671DOI Listing
September 2021

Autophagy-Related Three-Gene Prognostic Signature for Predicting Survival in Esophageal Squamous Cell Carcinoma.

Front Oncol 2021 15;11:650891. Epub 2021 Jul 15.

Department of Oncology, Cancer Institute, Peking University Shenzhen Hospital, Shenzhen Peking University-Hong Kong University of Science and Technology (PKU-HKUST) Medical Center, Shenzhen, China.

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive malignant tumors in China, and its prognosis remains poor. Autophagy is an evolutionarily conserved catabolic process involved in the occurrence and development of ESCC. In this study, we described the expression profile of autophagy-related genes (ARGs) in ESCC and developed a prognostic prediction model for ESCC patients based on the expression pattern of ARGs. We used four ESCC cohorts, GSE53624 (119 samples) set as the discovery cohort, The Cancer Genome Atlas (TCGA) ESCC set (95 samples) as the validation cohort, 155 ESCC cohort, and Oncomine cohort were used to screen and verify differentially expressed ARGs. We identified 34 differentially expressed genes out of 222 ARGs. In the discovery cohort, we divided ESCC patients into three groups that showed significant differences in prognosis. Then, we analyzed the prognosis of 34 differentially expressed ARGs. Three genes [poly (ADP-ribose) polymerase 1 (PARP1), integrin alpha-6 (ITGA6), and Fas-associated death domain (FADD)] were ultimately obtained through random forest feature selection and were constructed as an ARG-related prognostic model. This model was further validated in TCGA ESCC set. Cox regression analysis confirmed that the three-gene signature was an independent prognostic factor for ESCC patients. This signature effectively stratified patients in both discovery and validation cohorts by overall survival ( = 5.162E-8 and = 0.052, respectively). We also constructed a clinical nomogram with a concordance index of 0.713 to predict the survival possibility of ESCC patients by integrating clinical characteristics and the ARG signature. The calibration curves substantiated fine concordance between nomogram prediction and actual observation. In conclusion, we constructed a new ARG-related prognostic model, which shows the potential to improve the ability of individualized prognosis prediction in ESCC.
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http://dx.doi.org/10.3389/fonc.2021.650891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321089PMC
July 2021

Comment on "Associations Between Tea and Cancer Risk in Two Umbrella Reviews".

Adv Nutr 2021 07;12(4):1594

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.1093/advances/nmab042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321839PMC
July 2021

Circ_0000520 contributes to triple-negative breast cancer progression through mediating the miR-1296/ZFX axis.

Thorac Cancer 2021 Sep 29;12(18):2427-2438. Epub 2021 Jul 29.

Department of Breast Surgery, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi City, China.

Background: Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer with a high incidence of local recurrence and metastasis. Circular RNAs (circRNAs) are implicated in the pathomechanism of TNBC. Here, we investigated the function of circ_0000520 in TNBC and its associated mechanism.

Methods: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were used to measure RNA and protein expression. Cell proliferation was analyzed by cell counting kit-8 (CCK8) assay, flow cytometry and colony formation assay. Cell apoptosis was assessed by flow cytometry. Cell migration ability was analyzed by transwell migration and wound healing assays. Transwell invasion assay was conducted to analyze the invasion ability. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA-pulldown assay were performed to verify the interaction between microRNA-1296 (miR-1296) and circ_0000520 or zinc finger protein X-linked (ZFX). Xenograft mice model was established to analyze the role of circ_0000520 in xenograft tumor growth in vivo.

Results: Circ_0000520 expression was upregulated in TNBC tissues and cell lines. Circ_0000520 knockdown suppressed the proliferation, migration, and invasion whereas induced the apoptosis of TNBC cells. miR-1296 was verified as a target of circ_0000520, and circ_0000520 silencing-mediated suppressive effects on the malignant potential of TNBC cells were partly overturned by miR-1296 knockdown. miR-1296 interacted with the 3' untranslated region (3'UTR) of ZFX, and ZFX overexpression partly reversed miR-1296 overexpression-mediated effects in TNBC cells. Circ_0000520 absence reduced ZFX expression by upregulating miR-1296 in TNBC cells. Circ_0000520 silencing suppressed xenograft tumor growth in vivo.

Conclusions: Circ_0000520 contributed to TNBC development by binding to miR-1296 to induce ZFX expression.
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http://dx.doi.org/10.1111/1759-7714.14085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447912PMC
September 2021

Influence of patellar denervation on anterior knee pain and knee function following total knee replacement: A systematic review and meta-analysis.

ANZ J Surg 2021 Jul 21. Epub 2021 Jul 21.

Department of Orthopaedics, The People's Hospital, Guang'an, China.

Background: Previous studies have reached mixed results regarding the effects of patellar denervation with electrocautery (PD) on total knee replacement (TKR). This systematic review and meta-analysis aimed to summarize all available literatures to investigate the influence of PD on postoperative anterior knee pain (AKP) and knee function after TKR.

Methods: Electronic databases, including PubMed, Cochrane Library and Embase, were searched from their inception to March 2021. Randomized controlled trials (RCT) and quasi-randomized controlled trials (quasi-RCT) comparing PD and non-patellar denervation (NPD) in TKR were selected, and the Cochrane risk of bias tool was used to assess the quality of included trials. AKP prevalence was defined as the primary outcome.

Results: A total of 12 RCTs and one quasi-RCT enrolled 1895 knees proved eligible. PD knees had significantly lower AKP prevalence than NPD knees (odds ratio [OR] = 0.54; 95% confidence intervals [95% CI], 0.36-0.81; p = 0.003). There was no difference between PD and NPD in terms of visual analogue scale for knee pain and range of motion, American knee society knee score, American knee society function score, patellar feller score, Oxford knee score for knee function. The results of subgroup analysis based on follow-up duration and patella resurfacing were in accordance with the results. PD knees were not associated with a higher risk of complication or revision.

Conclusion: PD can significantly reduce the AKP prevalence following TKR without increasing the risk of complication and revision. Although the pain relief effect of PD may not be associated with improved knee function after TKR, this procedure is preferred in both patella resurfacing and patella non-resurfacing TKR.
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http://dx.doi.org/10.1111/ans.17078DOI Listing
July 2021

Plasma metabolites changes in male heroin addicts during acute and protracted withdrawal.

Aging (Albany NY) 2021 07 19;13(14):18669-18688. Epub 2021 Jul 19.

NHC Key Laboratory of Drug Addiction Medicine, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.

Background: Heroin addiction and withdrawal have been associated with an increased risk for infectious diseases and psychological complications. However, the changes of metabolites in heroin addicts during withdrawal remain largely unknown.

Methods: A total of 50 participants including 20 heroin addicts with acute abstinence stage, 15 with protracted abstinence stage and 15 healthy controls, were recruited. We performed metabolic profiling of plasma samples based on ultraperformance liquid chromatography coupled to tandem mass spectrometry to explore the potential biomarkers and mechanisms of heroin withdrawal.

Results: Among the metabolites analyzed, omega-6 polyunsaturated fatty acids (linoleic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid), omega-3 polyunsaturated fatty acids (docosahexaenoic acid, docosapentaenoic acid), aromatic amino acids (phenylalanine, tyrosine, tryptophan), and intermediates of the tricarboxylic acid cycle (oxoglutaric acid, isocitric acid) were significantly reduced during acute heroin withdrawal. Although majority of the metabolite changes could recover after months of withdrawal, the levels of alpha-aminobutyric acid, alloisoleucine, ketoleucine, and oxalic acid do not recover.

Conclusions: In conclusion, the plasma metabolites undergo tremendous changes during heroin withdrawal. Through metabolomic analysis, we have identified links between a framework of metabolic perturbations and withdrawal stages in heroin addicts.
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http://dx.doi.org/10.18632/aging.203311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351709PMC
July 2021
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