Publications by authors named "Yong Zhao"

1,695 Publications

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cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.

Protein Cell 2021 Oct 22. Epub 2021 Oct 22.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China.

As a sensor of cytosolic DNA, the role of cyclic GMP-AMP synthase (cGAS) in innate immune response is well established, yet how its functions in different biological conditions remain to be elucidated. Here, we identify cGAS as an essential regulator in inhibiting mitotic DNA double-strand break (DSB) repair and protecting short telomeres from end-to-end fusion independent of the canonical cGAS-STING pathway. cGAS associates with telomeric/subtelomeric DNA during mitosis when TRF1/TRF2/POT1 are deficient on telomeres. Depletion of cGAS leads to mitotic chromosome end-to-end fusions predominantly occurring between short telomeres. Mechanistically, cGAS interacts with CDK1 and positions them to chromosome ends. Thus, CDK1 inhibits mitotic non-homologous end joining (NHEJ) by blocking the recruitment of RNF8. cGAS-deficient human primary cells are defective in entering replicative senescence and display chromosome end-to-end fusions, genome instability and prolonged growth arrest. Altogether, cGAS safeguards genome stability by controlling mitotic DSB repair to inhibit mitotic chromosome end-to-end fusions, thus facilitating replicative senescence.
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http://dx.doi.org/10.1007/s13238-021-00879-yDOI Listing
October 2021

Spacer-based gap balancing is useful in total knee arthroplasty: a 3-year follow-up of a retrospective study.

J Orthop Surg Res 2021 Oct 21;16(1):633. Epub 2021 Oct 21.

Department of Orthopaedics, Liaocheng People's Hospital, 67 Dongchang West Road, Liaocheng, 252000, Shandong, China.

Background: Which technique, gap balancing or measured resection, can obtain better femoral component alignment and soft tissue balance in total knee arthroplasty (TKA) is still controversial. This study aimed to determine whether the gap balancing technique using a modified spacer block in TKA can result in better postoperative clinical outcomes than the measured resection technique.

Methods: A total of 124 patients who underwent consecutive primary TKA between May 2016 and August 2018 were retrospectively reviewed. The gap balancing technique assisted by a modified spacer block was used in 61 patients, and the measured resection technique was used in 63 patients. The surgical, imaging and knee function outcomes of the two groups were compared.

Results: The thickness of the posterior medial condyle bone resection using the modified spacer block tool in gap balancing was significantly larger than that of the MR technique (P = 0.001). Compared with the measured resection group, the gap balancing group had a greater external rotation resection angle of the femur (4.06 ± 1.10° vs. 3.19 ± 0.59°, P < 0.001°). Despite these differences, the mean ROM, KSS scores, and WOMAC scores at the 6-week, 1-year, and 2-year follow-ups were not significantly different. Postoperatively, there was no significant difference between the two groups in mechanical axis measurements (P = 0.275), the number of HKA outliers (P = 0.795) or the joint line displacement (P = 0.270).

Conclusion: The functional outcomes of the gap balancing technique based on the modified spacer are similar to those of measured resection at 3 years. Compared with the MR technique, the GB technique resulted in a greater external rotation resection angle and thicker posterior medial condylar cuts in TKA with knee varus.
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http://dx.doi.org/10.1186/s13018-021-02788-6DOI Listing
October 2021

A current collect-free Li1.2Ni0.13Co0.13Mn0.54O2 flexible film for high-performance lithium-ion batteries.

Nanotechnology 2021 Oct 15. Epub 2021 Oct 15.

Department of Physics, Nanchang University, 999 Xuefu Avenue, Honggutan District, Nanchang City, Jiangxi Province, Nanchang, Jiangxi, 330031, CHINA.

Due to the high demand for more convenient flexible devices, there are more requirements for higher performance of flexible batteries. The layered lithium-rich manganese-based Li1.2Ni0.13Co0.13Mn0.54O2 cathode material has the advantages of higher energy density, higher discharge capacity and environmentally friendly, so it can be used for high-performance flexible electrode cathode material. Its theoretical capacity can reach more than 250mAh g-1, which is higher than most cathode materials currently used in commercialization. Here we synthesize Li1.2Ni0.13Co0.13Mn0.54O2 (LNCM) cathode, and then use a simple method to make a current collect-free LNCM flexible film. This film has excellent flexibility and electrochemical performance. At 25 mA g-1, its initial discharge capacity reaches 314.0 mAh g-1. After 200 cycles of 500 mA g-1, its capacity retention rate is 82.1%, the attenuation is about 0.08% per cycle. Moreover, by bending at any position of the flexible film, it can still remain intact, and the soft-packaged battery made by the flexible film can still be used under the bending condition and keep the brightness of the LED lamp unchanged. This shows that using Li1.2Ni0.13Co0.13Mn0.54O2 to make high-performance flexible electrodes is a simple and effective method, which is expected to be practically applied to flexible electronic devices.
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http://dx.doi.org/10.1088/1361-6528/ac302aDOI Listing
October 2021

The effect and safety of levodopa alone versus levodopa sparing therapy for early Parkinson's disease: a systematic review and meta-analysis.

J Neurol 2021 Oct 15. Epub 2021 Oct 15.

Department of Neurosurgery, The Second Affiliated Hospital, Chongqing Medical University, 76 Linjiang Road, Chongqing, 400010, China.

Background: The best choice between levodopa alone and levodopa sparing medications for early Parkinson's disease (PD) remains controversial. We aimed to evaluate the effect and safety of levodopa alone and levodopa sparing therapy in symptom relief, neuroimage results and complications.

Methods: A systematic search was performed in PubMed, The Cochrane Library, EMBASE, and Web of Science for randomized controlled trials of early PD patients comparing levodopa-alone with levodopa-sparing therapy. The mean difference (MD) and the risk ratio (RR) were meta-analyzed.

Results: Twenty-three articles with 4913 patients were included. Significantly greater benefit was detected for the levodopa group in the changes of Unified Parkinson's Disease Rating Scale part II (p < 0.00001), III (p < 0.00001), and total (p < 0.00001) scores, and the between-group MD in part III score increased over time. The loss of the radioligands uptake in levodopa-alone group was also increasingly greater over time. Patients treated with levodopa alone were at higher risk for wearing-off (p < 0.001) and dyskinesia (p < 0.001), but the RR for dyskinesia between the two groups decreased after 2 years of follow-up.

Conclusion: Levodopa-alone therapy might be superior in motor symptom relief than levodopa-sparing therapy for early PD patients, and the motor advantage of levodopa-alone might grow over time. Sparing therapy might be associated with less risk of wearing-off and dyskinesia, but the events between the two groups might not be different in the long run. Overall, levodopa alone therapy might bring more net benefit to early PD patients compared with levodopa sparing strategies. The clinical and imaging findings are conflicting, which requires further investigation.
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http://dx.doi.org/10.1007/s00415-021-10830-0DOI Listing
October 2021

ABO multiferroic perovskite materials for memristive memory and neuromorphic computing.

Nanoscale Horiz 2021 Oct 15. Epub 2021 Oct 15.

School of Artificial Intelligence and School of Materials and Energy, Southwest University, Chongqing 400715, China.

The unique electron spin, transfer, polarization and magnetoelectric coupling characteristics of ABO multiferroic perovskite materials make them promising candidates for application in multifunctional nanoelectronic devices. Reversible ferroelectric polarization, controllable defect concentration and domain wall movement originated from the ABO multiferroic perovskite materials promotes its memristive effect, which further highlights data storage, information processing and neuromorphic computing in diverse artificial intelligence applications. In particular, ion doping, electrode selection, and interface modulation have been demonstrated in ABO-based memristive devices for ultrahigh data storage, ultrafast information processing, and efficient neuromorphic computing. These approaches presented today including controlling the dopant in the active layer, altering the oxygen vacancy distribution, modulating the diffusion depth of ions, and constructing the interface-dependent band structure were believed to be efficient methods for obtaining unique resistive switching (RS) behavior for various applications. In this review, internal physical dynamics, preparation technologies, and modulation methods are systemically examined as well as the progress, challenges, and possible solutions are proposed for next generation emerging ABO-based memristive application in artificial intelligence.
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http://dx.doi.org/10.1039/d1nh00292aDOI Listing
October 2021

Systematic review and meta-analysis: Prevalence of diabetes among patients with tuberculosis in China.

Trop Med Int Health 2021 Oct 12. Epub 2021 Oct 12.

Global Health Research Center, Duke Kunshan University, Kunshan, China.

Objective: Tuberculosis (TB) and diabetes mellitus (DM) are both significant public health problems in China. Results of previous studies on the prevalence of DM among TB patients in China are inconsistent. We conducted this systematic review and meta-analysis to estimate the overall prevalence of DM among TB patients in China.

Methods: We systematically searched Excerpta Medica Database, PubMed, Global Health, Ovid MEDLINE(R), Chinese Biomedical Literature Service System and China Online Journals - Health & Medicine and included all observational studies reporting prevalence of DM among TB patients in China. The Cochran's Q-statistic and I were used to test heterogeneity. Finally, a random-effect model meta-analysis was conducted to estimate the pooled prevalence of DM among TB patients in China using R studio.

Results: We screened 7043 articles and identified 43 eligible studies. The pooled prevalence was 7.8% (95% prediction interval 1.6-30.5, I =99.2%). The highest prevalence was observed in Northeast China (21.9%) among four economic regions of China, followed by East Coast (8.3%), Western China (5.9%), with Central China having the lowest prevalence (5.1%). Higher prevalence was observed in urban (10.1%) than in rural (7.8%) areas, and in hospital-based (9.0%) than in population-based studies (6.9%).

Conclusions: This study suggests that the prevalence of DM among TB patients in China aligns with the overall DM prevalence among the public in China, and public health strategies to reduce the burden of TB-DM comorbidity and inequity are needed.
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http://dx.doi.org/10.1111/tmi.13686DOI Listing
October 2021

Robust adaptive filtering algorithms based on (inverse)hyperbolic sine function.

PLoS One 2021 11;16(10):e0258155. Epub 2021 Oct 11.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, Center for Information in Medicine, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Recently, adaptive filtering algorithms were designed using hyperbolic functions, such as hyperbolic cosine and tangent function. However, most of those algorithms have few parameters that need to be set, and the adaptive estimation accuracy and convergence performance can be improved further. More importantly, the hyperbolic sine function has not been discussed. In this paper, a family of adaptive filtering algorithms is proposed using hyperbolic sine function (HSF) and inverse hyperbolic sine function (IHSF) function. Specifically, development of a robust adaptive filtering algorithm based on HSF, and extend the HSF algorithm to another novel adaptive filtering algorithm based on IHSF; then continue to analyze the computational complexity for HSF and IHSF; finally, validation of the analyses and superiority of the proposed algorithm via simulations. The HSF and IHSF algorithms can attain superior steady-state performance and stronger robustness in impulsive interference than several existing algorithms for different system identification scenarios, under Gaussian noise and impulsive interference, demonstrate the superior performance achieved by HSF and IHSF over existing adaptive filtering algorithms with different hyperbolic functions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258155PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504740PMC
October 2021

Multistate active control RCS signature for the continuous adjustment absorber/reflector transformation applications.

Opt Express 2021 Jul;29(15):24151-24160

Smart structures with tunable electromagnetic characteristics are required for camouflaging high-value targets, such as land warfare moving equipment, in continuously changing complex electromagnetic environments. The targets must control their radar cross section (RCS) to avoid detection and tracking. Frequency selective surfaces (FSSs) are the next-generation smart structures in which active RCS control is realized via impedance loading. In this paper, a multistate transformable FSS absorber/reflector that operates in the 3.9-11.0 GHz band is introduced and analyzed. The reflectivity amplitude of this absorber/reflector could be smoothly changed from 0 to -10 dB in 6.0-8.0 GHz. Each unit cell of the FSS structure consisted of four symmetrical diamond-shaped patterns, and the adjacent units were connected by PIN diodes. The absorption intensity of the FSS could be changed from 0 to -10 dB by adjusting the PIN bias voltage, which was applied via a simple bias network. The multistate switching characteristic of the FSS was verified by simulations and measurements. The results showed that adjustable absorbing intensity and switchable working states are the desirable characteristics that allow high-value targets to adapt to changing electromagnetic environments. Our work will bridge the gap between the available stealth strategies and practical applications, such as moving stealth vehicles.
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http://dx.doi.org/10.1364/OE.431941DOI Listing
July 2021

Genetic Diversity and Drug Susceptibility Profiles of Multidrug-Resistant Tuberculosis Strains in Southeast China.

Infect Drug Resist 2021 28;14:3979-3989. Epub 2021 Sep 28.

Department of Bacteriology and Immunology, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, People's Republic of China.

Background: Multidrug-resistant tuberculosis (MDR-TB) isolates collected from Fujian province, China were assessed for molecular epidemiological characteristics. Analysis of isolate genotype profiles revealed that the Beijing genotype was associated with especially high drug resistance and community transmission rates.

Methods: A total of 119 MDR-TB isolates obtained from TB patients in Fujian province were typed using 24-locus mycobacterium interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and spoligotyping. Drug susceptibility testing of all isolates was conducted using the L-J proportion method, with pyrazinamide (PZA) susceptibility testing conducted using the Mycobacterium Growth Indicator Tube System 960 (MGIT 960).

Results: We obtained 26 spoligotypes for the 119 isolates examined in this work. Spoligotyping results revealed that 80 (67.2%) isolates possessed the Beijing family genotypic profiles. Patients aged 25-44 years and ≥45 years were most likely to be infected by non-Beijing genotypes. The percentage of clustered cases with both PZA and ofloxacin (OFLX) resistance was significantly greater than the corresponding percentage for non-clustered cases. Of 44 PZA-resistant isolates, 28 isolates (63.6%) harbored mutations, while mutations were only detected in 7 (9.3%) PZA-susceptible isolates.

Conclusion: Our data demonstrate that the Beijing genotype is the dominant lineage among MDR-TB strains circulating in Fujian. Thus, MDR-TB infections occurring within this province are not likely associated with recent transmission events. PZA and fluoroquinolone resistance profiles were found to be associated with clustered isolates. Mutation of is the main driver of MDR-TB PZA resistance and is associated with mutation sites scattered throughout the entire protein-coding region.
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http://dx.doi.org/10.2147/IDR.S331516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487280PMC
September 2021

Diffusion adaptive filtering algorithm based on the Fair cost function.

Sci Rep 2021 Oct 5;11(1):19715. Epub 2021 Oct 5.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, Center for Information in Medicine, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

To better perform distributed estimation, this paper, by combining the Fair cost function and adapt-then-combine scheme at all distributed network nodes, a novel diffusion adaptive estimation algorithm is proposed from an M-estimator perspective, which is called the diffusion Fair (DFair) adaptive filtering algorithm. The stability of the mean estimation error and the computational complexity of the DFair are theoretically analyzed. Compared with the robust diffusion LMS (RDLMS), diffusion Normalized Least Mean M-estimate (DNLMM), diffusion generalized correntropy logarithmic difference (DGCLD), and diffusion probabilistic least mean square (DPLMS) algorithms, the simulation experiment results show that the DFair algorithm is more robust to input signals and impulsive interference. In conclusion, Theoretical analysis and simulation results show that the DFair algorithm performs better when estimating an unknown linear system in the changeable impulsive interference environments.
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http://dx.doi.org/10.1038/s41598-021-99330-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492734PMC
October 2021

Epigenetic Silencing of BMP6 by the SIN3A-HDAC1/2 Repressor Complex Drives Melanoma Metastasis via FAM83G/PAWS1.

Mol Cancer Res 2021 Oct 5. Epub 2021 Oct 5.

Center for Discovery and Innovation, Hackensack University Medical Center, Nutley, New Jersey.

Aberrant epigenetic transcriptional regulation is linked to metastasis, a primary cause of cancer-related death. Dissecting the epigenetic mechanisms controlling metastatic progression may uncover important insights to tumor biology and potential therapeutic targets. Here, we investigated the role of the SIN3A histone deacetylase 1 and 2 (SIN3A-HDAC1/2) complex in cancer metastasis. Using a mouse model of melanoma metastasis, we found that the SIN3A-HDAC1/2 transcription repressor complex silences BMP6 expression, causing increased metastatic dissemination and tumor growth via suppression of BMP6-activated SMAD5 signaling. We further discovered that FAM83G/PAWS1, a downstream effector of BMP6-SMAD5 signaling, contributes critically to metastatic progression by promoting actin-dependent cytoskeletal dynamics and cell migration. Pharmacologic inhibition of the SIN3A-HDAC1/2 complex reduced the numbers of melanoma cells in the circulation and inhibited metastatic tumor growth by inducing disseminated cell dormancy, highlighting the SIN3A-HDAC1/2 repressor complex as a potential therapeutic target for blocking cancer metastasis. IMPLICATIONS: This study identifies the novel molecular links in the metastatic progression to target cytoskeletal dynamics in melanoma and identifies the SIN3A-HDAC1/2 complex and FAM83G/PAWS1 as potential targets for melanoma adjuvant therapy.
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http://dx.doi.org/10.1158/1541-7786.MCR-21-0289DOI Listing
October 2021

Exercise promotes angiogenesis by enhancing endothelial cell fatty acid use via liver-derived extracellular vesicle miR-122-5p.

J Sport Health Sci 2021 Oct 1. Epub 2021 Oct 1.

School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.

Background: Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects. Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs (miRNAs) after exercise and found that some of them act as exerkines. However, whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.

Methods: A 9-day treadmill training was used as an exercise model in C57BL/6 mice. Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p (miR-122-5p). Human umbilical vein endothelial cells were used in vitro.

Results: Among these differentially expressed extracellular vesicle miRNAs, miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro. Exercise increased circulating levels of miR-122-5p, which was produced mainly by the liver and shuttled by extracellular vesicles in mice. Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles, and exercise-improved muscle performance in mice. Mechanistically, miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells, and miR-122-5p upregulated endothelial cell fatty-acid use by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase (AGPAT1). In addition, miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.

Conclusion: These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p, which enhances fatty acid use by targeting AGPAT1 in endothelial cells, highlighting the therapeutic potential of miR-122-5p in tissue repair.
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http://dx.doi.org/10.1016/j.jshs.2021.09.009DOI Listing
October 2021

LncRNA HIF1A-AS1 promotes gemcitabine resistance of pancreatic cancer by enhancing glycolysis through modulating the AKT/YB1/HIF-1α pathway.

Cancer Res 2021 Sep 30. Epub 2021 Sep 30.

Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

Gemcitabine (GEM) resistance is a major challenge for chemotherapy of pancreatic cancer (PC). Previous studies have reported on the role of lncRNA in tumorigenesis of PC, however, the involvement of lncRNA in the development of GEM resistance of PC remains unclear. In the present study, we demonstrated that the antisense RNA1 of HIF-1α (HIF1A-AS1) was significantly elevated in the GEM-resistant PC cells. Gain- and lost-of-function experiments validated that HIF1A-AS1 promoted GEM resistance of PC cells both in vitro and vivo. We further revealed that HIF1A-AS1 upregulated HIF-1α expression and thus promoted glycolysis to enhance GEM resistance of PC cells. Mechanistically, HIF1A-AS1 facilitated the interaction between serine/threonine kinase AKT and Y-box binding protein 1 (YB1), which promoted phosphorylation of YB1 (pYB1). Meanwhile, HIF1A-AS1 recruited pYB1 to HIF-1α mRNA which consequently promoted translation of HIF-1α. Furthermore, HIF-1α promoted HIF1A-AS1 transcription by directly binding to the HIF-1α response element in the promoter area of HIF1A-AS1 to form a positive feedback. Consistently, both HIF1A-AS1 and HIF-1α were upregulated in PC tissues and associated with poor overall survival. Together, our results underline a reciprocal loop of HIF1A-AS1 and HIF-1α which contributes to GEM resistance of PC and indicate that HIF1A-AS1 might serve as a novel therapeutic target for GEM resistance of PC.
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http://dx.doi.org/10.1158/0008-5472.CAN-21-0281DOI Listing
September 2021

Prevalence of anxiety and its associated factors among infertile patients after 'two-child' policy in Chongqing, China: a cross-sectional study.

Reprod Health 2021 Sep 30;18(1):193. Epub 2021 Sep 30.

Department of Reproductive Center, The First Affiliated Hospital of Chongqing Medical University, Youyi Road, Yuzhong, Chongqing, China.

Background: With the prevalence of infertility increasing every year around the world, it has seriously impacted the individual quality of family and social life. Anxiety is one of the most prevalent anxiety disorders among infertile patients. After the two-child policy, whether it affected the prevalence of anxiety is controversial. This study aimed to determine the prevalence of anxiety and its potential risk factors among Chinese infertile women after the enforcement of 'two-child policy'.

Methods: This cross-sectional study included 693 infertile patients in a reproductive medical center in Chongqing, China, between February 2016 and December 2018. Data was collected by Self-filling questionnaires including basic demographic information and the Generalized Anxiety Disorder-7 (GAD-7). SPSS statistical software (IBM SPSS version 25) was used to analyse the obtained data. Descriptive analysis was used to describe basic information and anxiety scores, the chi-square test and binary logistic regression were used to analyse the relationship between anxiety and other variables.

Results: The prevalence of anxiety among total infertile patients was 21.8%, and its 23.5% among first-child infertile patients (FI), and 18.4% among second-child infertile patients (SI) respectively (P > 0.05). Binary logistic regression showed that patients with lower education levels were more likely to have anxiety (P < 0.01). Patients with middle salary incomes were more likely to have anxiety (OR = 1.860, 95% CI: 1.068-3.238). Oral contraception taking history (OR = 1.778, 95% CI: 1.186-2.667), and history of allergy (OR = 2.098, 95% CI: 1.219-3.612) were associated with anxiety.

Conclusions: Under the full liberalization of the "two-child policy", the total prevalence of anxiety among Chinese infertile female is comparatively high. Low education levels, middle incomes, oral contraception taking and allergy history can be the related risk factors of anxiety. We promote that all infertile patients should be evaluated for the prevalence of anxiety, especially those with potential risks, and receive consultant or targeted treatment when needed.
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http://dx.doi.org/10.1186/s12978-021-01140-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482576PMC
September 2021

Nitrogen distribution and evolution during persulfate assisted hydrothermal carbonization of spirulina.

Bioresour Technol 2021 Sep 20;342:125980. Epub 2021 Sep 20.

College of Forestry, Henan Agricultural University, Zhengzhou, China; Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu 611756, China. Electronic address:

In this study, persulfate was used during hydrothermal processing of spirulina (160℃-220℃) for enhancement of nitrogen conversion. The nitrogen distribution in aqueous phase, hydrochar and biocrude-oil was evaluated, and the elemental composition and chemical forms of hydrochar were investigated. Results suggested that the addition of persulfate during hydrothermal processing of spirulina increased the atomic N/O of hydrochar for 1.2%-2.4%, whereas the NH-N concentration in liquid phase increased by approximately 67-155 mg/L regardless of temperature, suggesting that the persulfate could facilitate the organic nitrogen degradation and protein deamination. The N1s XPS analysis indicated that the protein-N, pyrrole-N, and inorganic-N ratio in spirulina were decreased, while more pyridine-N in hydrochar was formed, suggesting that more stable N forms were generated. In addition, the elementary composition also showed that more N was formed on the surface of hydrochar instead of the core.
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http://dx.doi.org/10.1016/j.biortech.2021.125980DOI Listing
September 2021

X-ray Pulsar Signal Denoising Based on Variational Mode Decomposition.

Entropy (Basel) 2021 Sep 8;23(9). Epub 2021 Sep 8.

School of Automation Science and Electrical Engineering, Beihang University (BUAA), Beijing 100191, China.

Pulsars, especially X-ray pulsars detectable for small-size detectors, are highly accurate natural clocks suggesting potential applications such as interplanetary navigation control. Due to various complex cosmic background noise, the original pulsar signals, namely photon sequences, observed by detectors have low signal-to-noise ratios (SNRs) that obstruct the practical uses. This paper presents the pulsar denoising strategy developed based on the variational mode decomposition (VMD) approach. It is actually the initial work of our interplanetary navigation control research. The original pulsar signals are decomposed into intrinsic mode functions (IMFs) via VMD, by which the Gaussian noise contaminating the pulsar signals can be attenuated because of the filtering effect during signal decomposition and reconstruction. Comparison experiments based on both simulation and HEASARC-archived X-ray pulsar signals are carried out to validate the effectiveness of the proposed pulsar denoising strategy.
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http://dx.doi.org/10.3390/e23091181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465729PMC
September 2021

Chiral Hybrid Perovskite Single-Crystal Nanowire Arrays for High-Performance Circularly Polarized Light Detection.

Adv Sci (Weinh) 2021 Sep 24:e2102065. Epub 2021 Sep 24.

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Circularly polarized light (CPL) detection has emerged as a key technology for various optoelectronics. Chiral hybrid perovskites (CHPs) that combine CPL-sensitive absorption induced by chiral organic ligands and superior photoelectric properties of perovskites are promising candidates for direct CPL detection. To date, most of the CHP detectors are made up of polycrystalline thin-film, which results in a rather limited discrimination of CPL due to the existence of redundant impurities and intrinsic defect states originating from rapid crystallization process. Here, it is developed a direct CPL detector with high photocurrent and polarization selectivity based on low-defect CHP single-crystal nanowire arrays. Large-scale CHP nanowires are obtained through a micropillar template-assisted capillary-bridge rise approach. Thanks to the high crystallinity and ordered crystallographic alignment of these arrays, a CPL photodetector with high light on/off ratio of 1.8 × 10 , excellent responsivity of 1.4 A W , and an outstanding anisotropy factor of 0.24 for photocurrent has been achieved. These results would provide useful enlightenment for direct CPL detection in high-performance chiral optoelectronics.
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http://dx.doi.org/10.1002/advs.202102065DOI Listing
September 2021

Evaluating the mitigation strategies of COVID-19 by the application of the CO emission data through high-resolution agent-based computational experiments.

Environ Res 2021 Sep 21:112077. Epub 2021 Sep 21.

College of Systems Engineering, National University of Defense Technology (NUDT), 410073, 109 Deya Road, Kaifu District, Changsha City, Hunan province, China. Electronic address:

The negative consequences, such as healthy and environmental issues, brought by rapid urbanization and interactive human activities result in increasing social uncertainties, unreliable predictions, and poor management decisions. For instance, the Coronavirus Disease (COVID-19) occurred in 2019 has been plaguing many countries. Aiming at controlling the spread of COVID-19, countries around the world have adopted various mitigation and suppression strategies. However, how to comprehensively eva luate different mitigation strategies remains unexplored. To this end, based on the Artificial societies, Computational experiments, Parallel execution (ACP) approach, we proposed a system model, which clarifies the process to collect the necessary data and conduct large-scale computational experiments to evaluate the effectiveness of different mitigation strategies. Specifically, we established an artificial society of Wuhan city through geo-environment modeling, population modeling, contact behavior modeling, disease spread modeling and mitigation strategy modeling. Moreover, we established an evaluation model in terms of the control effects and economic costs of the mitigation strategy. With respect to the control effects, it is directly reflected by indicators such as the cumulative number of diseases and deaths, while the relationship between mitigation strategies and economic costs is built based on the CO emission. Finally, large-scale simulation experiments are conducted to evaluate the mitigation strategies of six countries. The results reveal that the more strict mitigation strategies achieve better control effects and less economic costs.
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http://dx.doi.org/10.1016/j.envres.2021.112077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452542PMC
September 2021

The Effects of Adoptively Transferred IL-23/IL-18-Polarized Neutrophils on Tumor and Collagen-Induced Arthritis in Mice.

J Inflamm Res 2021 16;14:4669-4686. Epub 2021 Sep 16.

Department of State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.

Background: Neutrophils present great diverse phenotypes in various microenvironments and play different immune regulatory functions. Neutrophils generally classified into inflammatory phenotype N1 and anti-informatory phenotype N2. Our recent studies showed that IL-23 alone stimulated neutrophils to express IL-17A, IL-17F and IL-22 and displayed a gene transcriptional profile similar to Th17 cells. In the present study, we tried to identify potential cytokines to promote IL-23-induced neutrophil polarization.

Methods: Mouse bone marrow-derived neutrophils and human peripheral blood neutrophils were treated with IL-23 (10 ng/mL) plus IL-18 (25 ng/mL) to induce Th17-like subset in vitro and detected by real-time PCR, flow cytometry, ELISA, immunofluorescence and RNA-seq assays. In vivo, collagen-induced arthritis (CIA) mouse model and EL4 tumor-bearing mouse model were used to characterize the potential roles of N(IL-23+IL-18) in inflammation and tumor.

Results: Real-time PCR, ELISA and flow cytometry assays showed that IL-18 could significantly enhance IL-23-induced IL-17A, IL-17F and IL-22 expressions in mouse and human neutrophils in a synergistic way, although IL-18 alone failed to induce these cytokines expression. RNA-seq and molecular studies showed that the polarization of N(IL-23+IL-18) is mainly mediated by the JNK/p38-STAT3-BATF signaling pathway. Adoptive transfer of the induced N(IL-23+IL-18) neutrophils significantly accelerated the tumor growth in EL4 tumor-bearing mice and enhanced disease progression in the CIA mouse model. IL-17A-deficient N(IL-23+IL-18) neutrophils failed to enhance the CIA pathogenesis in this model, suggesting that IL-17A may be involved in the N(IL-23+IL-18) neutrophils-promoted arthritis in mice.

Conclusion: The Th17-type subpopulation N(IL-23+IL-18) has pro-tumor and pro-inflammatory properties. Recognizing the different functional polarization of neutrophils would significantly help us to understand the distinctive protective/pathological roles of neutrophils in physiological and different pathological situations.
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http://dx.doi.org/10.2147/JIR.S329528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453247PMC
September 2021

Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials.

mSystems 2021 Sep 21:e0072521. Epub 2021 Sep 21.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

The neonatal period is a crucial time during development of the mammalian small intestine. Moreover, neonatal development and maturation of the small intestine are exceptionally important for early growth, successful weaning, and postweaning growth and development, in order to achieve species-specific milestones. Although several publications recently characterized intestinal epithelial cell diversity at the single-cell level, it remains unclear how differentiation and molecular interactions take place between types and subtypes of epithelial cells during the neonatal period. A single-cell RNA sequencing (scRNA-seq) survey of 40,186 ileal epithelial cells and proteomics analysis of ileal samples at 6 time points in the swine neonatal period were performed. The results revealed previously unknown developmental changes: specific increases in undifferentiated cells, unique enterocyte differentiation, and time-dependent reduction in secretory cells. Moreover, we observed specific transcriptional factors, ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and gut mucosal microbiota playing vital roles in ileal development during the neonatal window. This work offers new comprehensive information regarding ileal development throughout the neonatal period. Reference to this data set may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies. We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process. Our results may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies.
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http://dx.doi.org/10.1128/mSystems.00725-21DOI Listing
September 2021

6-Gingerol protects against cerebral ischemia/reperfusion injury by inhibiting NLRP3 inflammasome and apoptosis via TRPV1 / FAF1 complex dissociation-mediated autophagy.

Int Immunopharmacol 2021 Sep 17;100:108146. Epub 2021 Sep 17.

Department of Pathology, Chongqing Medical University, Chongqing 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China. Electronic address:

Background: Our previous studies demonstrated that autophagy alleviates cerebral I/R injury by inhibiting NLRP3 inflammasome-mediated inflammation. 6-Gingerol, a phenolic compound extracted from ginger, was reported to possess potent antiapoptotic and anti-inflammatory activities and is associated with autophagy. However, the effects of 6-Gingerol in cerebral I/R injury have not been elucidated, and whether they involve autophagy-induced NLRP3 inflammasome inhibition remains unclear.

Methods: Adult male Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion (MCAO) for 1 h, followed by reperfusion for 24 h. 6-Gingerol and 3-methyladenine (3-MA) were injected intraperitoneally, and si-TRPV1 was injected via the lateral ventricle. Cerebral infarct volume, brain edema, neurological deficits, HE and Nissl were used to evaluate the morphological and functional changes of brain tissue, respectively. TRPV1, FAF1, autophagy related (LC3II/I, P62, Beclin1), inflammation related (NLRP3, cleaved-caspase-1, caspase-1, cleaved-IL-1β, IL-1β, cleaved-IL-18, IL-18) and apoptosis related (Bcl-2, Bax, cleaved-caspase-3) proteins were assessed by Western blot, immunofluorescence staining and coimmunoprecipitation, respectively. Enzyme linked immunosorbent assay (ELISA) was used to evaluate the changes in the expression levels of interleukin-1 (IL-1β) and interleukin-18(IL-18), respectively. The degree of neuronal apoptosis was evaluated by TUNEL staining. Neuronal ultrastructure was examined by transmission electron microscopy.

Result: 6-Gingerol treatment significantly reduced cerebral infarct volume, improved brain edema and neurological scores, and reversed brain histomorphological damage after I/R injury. In addition, 6-Gingerol significantly reduced NLRP3 inflammasome-derived inflammation and neuronal apoptosis and upregulated autophagy. The autophagy inhibitor 3-MA rescued the effects of 6-Gingerol on the NLRP3 inflammasome and apoptosis. Moreover, the findings illustrated that 6-Gingerol inhibited autophagy-induced NLRP3 inflammasome activation and apoptosis through the dissociation of TRPV1 from FAF1.

Conclusion: In brief, 6-Gingerol exerts antiapoptotic and anti-inflammatory effects via TRPV1/FAF1 complex dissociation-mediated autophagy during cerebral I/R injury. Therefore, 6-Gingerol may be an effective drug for the treatment of I/R injury.
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http://dx.doi.org/10.1016/j.intimp.2021.108146DOI Listing
September 2021

The Development and Survival of Thymic Epithelial Cells Require TSC1-Dependent Negative Regulation of mTORC1 Activity.

J Immunol 2021 Oct 17;207(8):2039-2050. Epub 2021 Sep 17.

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China;

Thymic epithelial cells (TECs) are critical for the development and generation of functionally competent T cells. Until now, the mechanism that regulates the survival of TECs is poorly understood. In the current study, we found that controls the homeostasis of medullary TECs (mTECs) by inhibiting lysosomal-mediated apoptosis pathway in mice. TEC-specific deletion of predominately decreased the cell number of mTECs and, to a lesser content, affected the development cortical TECs. The defect of mTECs caused by deficiency in mice impaired thymocyte development and peripheral T cell homeostasis. Mechanistically, deficiency did not affect the cell proliferation of mTECs but increased the apoptosis of mTECs significantly. RNA-sequencing analysis showed that pathways involved in lysosomal biogenesis, cell metabolism, and apoptosis were remarkably elevated in -deficient mTECs compared with their wild-type counterparts. -deficient mTECs exhibited overproduction of reactive oxygen species and malfunction of lysosome, with lysosome membrane permeabilization and the release of cathepsin B and cathepsin L to the cytosol, which then lead to Bid cleaved into active truncated Bid and subsequently intrinsic apoptosis. Finally, we showed that the impaired development of mTECs could be partially reversed by decreasing mTORC1 activity via haploinsufficiency of Thus, is essential for the homeostasis of mTECs by inhibiting lysosomal-mediated apoptosis through mTORC1-dependent pathways.
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http://dx.doi.org/10.4049/jimmunol.2100463DOI Listing
October 2021

Cutaneous manifestations in children with SARS-CoV-2 infection and/or COVID-19: what do we know after 10 months under this pandemic?

Int J Dermatol 2021 Sep 16. Epub 2021 Sep 16.

Department of Clinical Laboratory, Children's Hospital Affiliated to Capital Institute of Pediatrics, Beijing, China.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global crisis. However, cutaneous manifestations in children with SARS-CoV-2 infection have received little attention.

Objective: Our study was aimed to present clinical and cutaneous manifestations of children with SARS-CoV-2 and to provide the basis for early diagnosis.

Methods: We analyzed the clinical data of COVID-19 infected (CI) children worldwide from December 2019 to October 6, 2020, by using search terms "COVID-19," "SARS-CoV-2," "Coronavirus" in combination with "cutaneous," "lesion," "rash," "skin," "dermatology," "epidermis," "dermis," "multisystem inflammatory syndrome." We collected and analyzed the general information, clinical symptoms, cutaneous manifestations, laboratory examination results, history of close contact with CI patients or suspected CI patients, and outcome in CI children.

Results: Among 90 CI children, there were 46 boys (59%) and 32 girls (41%). Erythema was the most frequent lesion, followed by conjunctivitis, and edematous lesions. Face was the most commonly affected location including lips, conjunctivae, tongue, eyes, and eyelids. Sixty-three (73.3%) patients had multisystem inflammatory syndrome (MIS-C). As for clinical symptoms, 70 (81.4%) CI children suffered fever, and 34 (39.5%) patients had lung involvement. Meanwhile, 65.1% of patients had cardiac involvement, 4.7% of patients were asymptomatic, and 71.6% of patients received intravenous immunoglobulin, as well as 31.1% of CI children received systemic corticosteroids. Three children were dead. The most frequent route of infection was familial clustering. As silent virus carriers of CI children, it is important to find out the clinical and cutaneous manifestations in CI children to prevent and control COVID-19 transmission.
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http://dx.doi.org/10.1111/ijd.15882DOI Listing
September 2021

[email protected] Core-Shell fibers as cathode host material for High-Performance Lithium-Sulfur batteries.

J Colloid Interface Sci 2021 Aug 28;607(Pt 1):655-661. Epub 2021 Aug 28.

Key Laboratory of Bioinspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry, Beihang University, Beijing 100191, PR China. Electronic address:

Owing to the low cost, high energy density, and high theoretical specific capacity, lithium-sulfur batteries have been deemed as a potential choice for future energy storage devices. However, they also have suffered from several scientific and technical issues including low conductivity, polysulfides migration, and volume changes. In this study, [email protected] core-shell fibers were fabricated through combination of coaxial electrospinning and selective vulcanization method. The core-shell fibers are able to efficiently host sulfur, confine polysulfides, and accelerate intermediates conversion. This electrode delivers an initial specific capacity of 1181.1 mAh g and a high capacity of 736.5 mAh g after 300 cycles with high coulombic efficiency over 99.5% (capacity decay of 0.06% per cycle). This strategy of isolating interactant and selective vulcanization provides new ideas for effectively constructing heterostructure materials for lithium-sulfur batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.08.171DOI Listing
August 2021

Parents' and adolescents' perceptions of parental involvement and their relationships with depression among Chinese middle school students during the COVID-19 pandemic.

Child Youth Serv Rev 2021 Oct 5;129:106190. Epub 2021 Aug 5.

Aoti Zhonglu Campus of the Middle School Affiliated to Shandong University, Jinan, China.

Although psychological stress that adolescents may encounter during the COVID-19 pandemic has been of increasing interest to scholars, few studies have examined the profound impact that parents give to adolescents when staying indoors. This study surveyed 1,550 students and their parents from eight middle schools in eastern China. We employed multiple linear regressions with school fixed effects to examine the different perceptions of parental involvement between parents and children, and the relationships between these different types of parental involvement and depression in middle school students. Results indicated that discrepancies existed in their perceptions of behavioral aspects of parental involvement, including parental academic involvement, parent-teacher communication, and parent-child communication. Most saliently, higher levels of parental academic involvement (B = 0.051,  < 0.05) and lower levels of parent-child communication (B = -0.084,  < 0.05) perceived by students were associated with higher levels of depression. These findings contribute to the understanding of the association between parental involvement and students' mental health during the COVID-19 pandemic.
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http://dx.doi.org/10.1016/j.childyouth.2021.106190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421081PMC
October 2021

Accumulated Lattice Strain as an Internal Trigger for Spontaneous Pathway Selection.

J Am Chem Soc 2021 Sep 10;143(37):15319-15325. Epub 2021 Sep 10.

RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Multicomponent crystallization is universally important in various research fields including materials science as well as biology and geology, and presents new opportunities in crystal engineering. This process includes multiple kinetic and thermodynamic events that compete with each other, wherein "external triggers" often help the system select appropriate pathways for constructing desired structures. Here we report an unprecedented finding that a lattice strain accumulated with the growth of a crystal serves as an "internal trigger" for pathway selection in multicomponent crystallization. We discovered a "spontaneous" crystal transition, where the kinetically preferred layered crystal, initially formed by excluding the pillar component, carries a single dislocation at its geometrical center. This crystal "spontaneously" liberates a core region to relieve the accumulated lattice strain around the dislocation. Consequently, the liberated part becomes dynamic and enables the pillar ligand to invade the crystalline lattice, thereby transforming into a thermodynamically preferred pillared-layer crystal.
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http://dx.doi.org/10.1021/jacs.1c06854DOI Listing
September 2021

Safety and Accuracy of Suction Rectal Biopsy in Preterm Infants.

Front Pediatr 2021 20;9:642342. Epub 2021 Aug 20.

Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Most pediatric surgeons give little attention to the diagnosis of Hirschsprung disease (HD) in preterm infants. We aimed to explore the safety and accuracy of suction rectal biopsy (SRB) for diagnosing HD in preterm infants. A retrospective review was conducted of 45 preterm patients who underwent SRB from 2015 to 2019 in our hospital. We collected the clinical characteristics and pathology results of the patients and information on follow-up. The sensitivity and specificity of SRB for HD diagnosis were calculated. The median gestational age of the patients was 35 weeks (range: 28.9-36.9 weeks), the median gestational age at biopsy was 38.6 weeks (range: 33.4-60.0 weeks), and the median weight was 2,790 g (range: 1,580-4,100 g). Fifteen patients (33.3%) were positive for HD, which was confirmed after pull-through surgery. Ganglion cells were present in 30 patients. The diagnosis of HD was excluded in 29 patients after discharge follow-up. The sensitivity of SRB ranged from 93.7 to 100%, and the specificity was 100%. No complications occurred after SRB among the patients whose biopsy age was <37 weeks (10 patients) or biopsy weight was <2,000 g (five patients). SRB is accurate and safe for diagnosing HD in late preterm infants.
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http://dx.doi.org/10.3389/fped.2021.642342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417823PMC
August 2021

Higher Dietary Se Intake Is Associated With the Risk of New-Onset Fracture: A National Longitudinal Study for 20 Years.

Front Nutr 2021 18;8:719147. Epub 2021 Aug 18.

School of Public Health and Management, Chongqing Medical University, Chongqing, China.

The association between dietary selenium (Se) intake and osteoporosis-related fractures remains inconsistent. We aimed to examine the dose relationship between Se intake and incident fracture among Chinese adults. The dietary data were retrieved from the China Health and Nutrition Survey conducted between 1991 and 2011, and 17,150 participants aged above 20 were included. A 3-day, 24-h recall of food intake was performed to assess cumulative average dietary Se intake. The fracture was based on self-report in each survey between 1997 and 2011. The association between Se intake and fracture was tested by Cox regression, and the non-linear association was examined by restricted cubic splines (RCS). There were 976 fracture cases during a mean of 10.2 years follow-up. In a fully adjusted Cox model, across the quartiles of Se intake, the hazard ratios (HRs) for fracture were 1.07 (95% CI .86-1.33), 1 (reference), 1.25 (95% CI 1.02-1.53), and 1.33 (95% CI 1.07-1.65). RCS showed a parabolic association ( non-linear = 0.037) between Se and fracture for men as well as a U-shape dose-response ( non-linear = 0.04) between Se and fracture for subjects living in highly urbanized areas. In conclusion, there is a non-linear association between selenium intake and fracture, with higher intake associated with increased risk. The shape of the association varies by gender and urbanization level.
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http://dx.doi.org/10.3389/fnut.2021.719147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416262PMC
August 2021

LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.

Cell Death Dis 2021 09 3;12(9):830. Epub 2021 Sep 3.

Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

The majority of long non-coding RNAs (lncRNAs) have been discovered to be overexpressed in pancreatic cancer (PC) and served as promoters in the tumorigenesis of PC, while the inhibitory functions of lncRNAs in the development of PC have not been fully elucidated yet. LncRNA microarray was adopted to analyze the differential expression of lncRNAs in PC tissues and that in normal peritumoral (NP) tissues. Functional role of lncRNA BM466146.1 on PC was evaluated by gain- and loss-of-function experiments in vivo and in vitro. RNA pull-down, RNA immunoprecipitation, luciferase reporter, and Chromatin-immunoprecipitation assays were performed to assess the mechanism of ZNFTR, respectively. The correlation between the expression of ZNFTR and various clinicopathological characteristics was accessed in PC specimens. This study displayed lncRNA BM466146.1 was downregulated in PC tissues and functioned as a suppressor through regulating the expression of adjacent gene Zinc finger protein 24 (ZNF24), which was assigned as ZNFTR. Mechanistically, ZNFTR interacted with activating transcription factor 3 (ATF3) and sequestered ATF3 away from the ZNF24 promoter, which consequently increased the expression of ZNF24. Further, ZNF24 inhibited the proliferative, metastatic, and pro-angiogenic abilities of PC cells by suppressing transcription of vascular endothelial growth factor A (VEGFA). Therefore, the downregulation of ZNFTR in PC led to the decreased expression of ZNF24, which further resulted in the upregulation of VEGFA to facilitate the development of PC. Meanwhile, ZNFTR was transcriptionally inhibited by the HIF-1α/HDAC1 complex-mediated deacetylation. Clinical results further demonstrated that the low expression of ZNFTR was associated with poor overall survival time. Taken together, our results implicated that ZNFTR was a hypoxia-responsive lncRNA, and functioned as an inhibitor by modulating ATF3/ZNF24/VEGFA pathway in PC.
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http://dx.doi.org/10.1038/s41419-021-04119-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417266PMC
September 2021

Randomly Induced Phase Transformation in Silk Protein-Based Microlaser Arrays for Anticounterfeiting.

Adv Mater 2021 Oct 3;33(42):e2102586. Epub 2021 Sep 3.

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Anticounterfeiting labels based on physical unclonable functions (PUFs) exhibit high security with unreplicable code outputs, making them an ideal platform to realize unbreakable anticounterfeiting. Although various schemes are proposed for PUF labels, the utilization of natural randomness suffers from unpredictable signal extraction sites, which poses a challenge to efficient and convenient authentication for practical anticounterfeiting applications. Here, a covert optical PUF-based cryptographic protocol from silk protein-based microlaser (SML) arrays that possess hidden randomness of lasers for unclonable lasing signals as well as a defined location for efficient identification is proposed. The initial SMLs are patterned by casting laser dye-doped regenerated silk fibroin solution, resulting in a uniform microlaser array with regulated positions. With the SML array as substrate, random methanol microdroplets are stochastically sprayed on the SML array, which eventually induces uneven lasing signal changes of the patterned microlasers. The treated SML array possesses the deterministic readout sites of laser signals and unrepeatable signal distribution characteristics, which can guarantee efficient authentication and high security when serving as an anticounterfeiting label.
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http://dx.doi.org/10.1002/adma.202102586DOI Listing
October 2021
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