Publications by authors named "Yong Zhang"

6,208 Publications

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Highly efficient blue quantum-dot light-emitting diodes based on a mixed composite of a carbazole donor and a triazine acceptor as the hole transport layer.

Phys Chem Chem Phys 2022 Jun 24. Epub 2022 Jun 24.

School of Semiconductor Science and Technology, South China Normal University, Guangzhou 510631, P. R. China.

Solution-processed thermally activated delayed fluorescence (TADF) exciplexes were employed as the hole transport layer (HTL) of blue quantum dot (QD) light-emitting diodes (QLEDs) by blending polymer donors of poly(N-vinylcarbazole) (PVK) with small molecular acceptors of 2,4,6-tris(biphenyl-3-yl)-1,3,5-triazine (T2T). As a result, the PVK:T2T HTL can harvest holes and electrons leaking from the QD active layer to form exciplex excitons and then this harvested exciton energy can be effectively transferred to the adjacent QD emitters through the Förster resonance energy-transfer process. Furthermore, the TADF exciplexes can enhance the hole mobility of the HTL due to the charge transfer process from the PVK donor to the T2T acceptor under an external electric field. The maximum current efficiency (CE) and external quantum efficiency (EQE) of the fabricated blue ZnCdS/ZnS core/shell QLEDs increase from 4.14 cd A and 7.33% for the PVK HTL to 7.73 cd A and 13.66% for the PVK:(5 wt%)T2T HTL, respectively. Our results demonstrate that the TADF exciplex HTL would be a facile strategy to design high-performance blue QLEDs.
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http://dx.doi.org/10.1039/d2cp01777fDOI Listing
June 2022

Apparent Diffusion Coefficient Map-Based Radiomics Features for Differential Diagnosis of Pleomorphic Adenomas and Warthin Tumors From Malignant Tumors.

Front Oncol 2022 7;12:830496. Epub 2022 Jun 7.

Department of MRI, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Purpose: The magnetic resonance imaging (MRI) findings may overlap due to the complex content of parotid gland tumors and the differentiation level of malignant tumor (MT); consequently, patients may undergo diagnostic lobectomy. This study assessed whether radiomics features could noninvasively stratify parotid gland tumors accurately based on apparent diffusion coefficient (ADC) maps.

Methods: This study examined diffusion-weighted imaging (DWI) obtained with echo planar imaging sequences. Eighty-eight benign tumors (BTs) [54 pleomorphic adenomas (PAs) and 34 Warthin tumors (WTs)] and 42 MTs of the parotid gland were enrolled. Each case was randomly divided into training and testing cohorts at a ratio of 7:3 and then was compared with each other, respectively. ADC maps were digitally transferred to ITK SNAP (www.itksnap.org). The region of interest (ROI) was manually drawn around the whole tumor margin on each slice of ADC maps. After feature extraction, the Synthetic Minority Oversampling TEchnique (SMOTE) was used to remove the unbalance of the training dataset. Then, we applied the normalization process to the feature matrix. To reduce the similarity of each feature pair, we calculated the Pearson correlation coefficient (PCC) value of each feature pair and eliminated one of them if the PCC value was larger than 0.95. Then, recursive feature elimination (RFE) was used to process feature selection. After that, we used linear discriminant analysis (LDA) as the classifier. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic performance of the ADC.

Results: The LDA model based on 13, 8, 3, and 1 features can get the highest area under the ROC curve (AUC) in differentiating BT from MT, PA from WT, PA from MT, and WT from MT on the validation dataset, respectively. Accordingly, the AUC and the accuracy of the model on the testing set achieve 0.7637 and 73.17%, 0.925 and 92.31%, 0.8077 and 75.86%, and 0.5923 and 65.22%, respectively.

Conclusion: The ADC-based radiomics features may be used to assist clinicians for differential diagnosis of PA and WT from MTs.
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http://dx.doi.org/10.3389/fonc.2022.830496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210443PMC
June 2022

Eliciting Anti-Cancer Immunity by Genetically Engineered Multifunctional Exosomes.

Mol Ther 2022 Jun 22. Epub 2022 Jun 22.

Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089; Department of Chemistry, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089; Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089; Research Center for Liver Diseases, University of Southern California, Los Angeles, CA 90089. Electronic address:

Exosomes are cell-derived nanovesicles involved in regulating intercellular communications. In contrast to conventional nanomedicines, exosomes are characterized by unique advantages for therapeutic development. Despite their major successes in drug delivery, the full potential of exosomes for immunotherapy remains untapped. Herein we designed genetically engineered exosomes featured with surfaced-displayed antibody targeting groups and immunomodulatory proteins. Through genetic fusions with exosomal membrane proteins, Expi293F cell-derived exosomes were armed with monoclonal antibodies specific for human T-cell CD3 and epidermal growth factor receptor (EGFR) as well as immune checkpoint modulators, programmed death 1 (PD-1) and OX40 ligand (OX40L). The resulting genetically engineered multifunctional immune-modulating exosomes (GEMINI-Exos) can not only redirect and activate T cells toward killing EGFR-positive triple negative breast cancer (TNBC) cells but also elicit robust anti-cancer immunity, giving rise to highly potent inhibition against established TNBC tumors in mice. GEMINI-Exos represent candidate agents for immunotherapy and may offer a general strategy for generating exosome-based immunotherapeutics with desired functions and properties.
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http://dx.doi.org/10.1016/j.ymthe.2022.06.013DOI Listing
June 2022

Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit ().

Int J Mol Sci 2022 Jun 19;23(12). Epub 2022 Jun 19.

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

Hardy kiwifruit () has recently become popular in fresh markets due to its edible skin and rich nutritional value. In the present study, different harvest stages of two cultivars, 'Issai' and 'Ananasnaya' ("Ana"), were chosen for investigating the effects of maturity on the quality of the fruit. Interestingly, Issai contained 3.34 folds higher ascorbic acid (AsA) content than Ana. The HPLC method was used to determine the AsA content of the two varieties and revealed that Issai had the higher content of AsA and DHA. Moreover, RNA sequencing (RNAseq) of the transcriptome-based expression analysis showed that 30 differential genes for ascorbate metabolic pathways were screened in Issai compared to Ana, which had 16 genes down-regulated and 14 genes up-regulated, while compared to the up-regulation of 8 transcripts encoding the key enzymes involved in the L-galactose biosynthesis pathway. Our results suggested that AsA was synthesized mainly through the L-galactose pathway in hardy kiwifruit.
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http://dx.doi.org/10.3390/ijms23126816DOI Listing
June 2022

Spatiotemporal Distribution and Evolution of Digestive Tract Cancer Cases in Lujiang County, China since 2012.

Int J Environ Res Public Health 2022 Jun 17;19(12). Epub 2022 Jun 17.

Key Laboratory of Earth Surface Processes and Response in the Yangtze-Huaihe River Basin, School of Geography and Tourism, Anhui Normal University, Wuhu 241002, China.

This study aims to analyze the spatiotemporal distribution and evolution of digestive tract cancer (DTC) in Lujiang County, China by using the geographic information system technology. Results of this study are expected to provide a scientific basis for effective prevention and control of DTC. The data on DTC cases in Lujiang County, China, were downloaded from the Data Center of the Center for Disease Control and Prevention in Hefei, Anhui Province, China, while the demographic data were sourced from the demographic department in China. Systematic statistical analyses, including the spatial empirical Bayes smoothing, spatial autocorrelation, hotspot statistics, and Kulldorff's retrospective space-time scan, were used to identify the spatial and spatiotemporal clusters of DTC. GM(1,1) and standard deviation ellipses were then applied to predict the future evolution of the spatial pattern of the DTC cases in Lujiang County. The results showed that DTC in Lujiang County had obvious spatiotemporal clustering. The spatial distribution of DTC cases increases gradually from east to west in the county in a stepwise pattern. The peak of DTC cases occurred in 2012-2013, and the high-case spatial clusters were located mainly in the northwest of Lujiang County. At the 99% confidence interval, two spatiotemporal clusters were identified. From 2012 to 2017, the cases of DTC in Lujiang County gradually shifted to the high-incidence area in the northwest, and the spatial distribution range experienced a process of "dispersion-clustering". The cases of DTC in Lujiang County will continue to move to the northwest from 2018 to 2025, and the predicted spatial clustering tends to be more obvious.
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http://dx.doi.org/10.3390/ijerph19127451DOI Listing
June 2022

Plant DNA Methylation Responds to Nutrient Stress.

Genes (Basel) 2022 May 31;13(6). Epub 2022 May 31.

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

Nutrient stress as abiotic stress has become one of the important factors restricting crop yield and quality. DNA methylation is an essential epigenetic modification that can effectively regulate genome stability. Exploring DNA methylation responses to nutrient stress could lay the foundation for improving plant tolerance to nutrient stress. This article summarizes the plant DNA methylation patterns, the effects of nutrient stress, such as nitrogen, phosphorus, iron, zinc and sulfur stress, on plant DNA methylation and research techniques for plant DNA methylation, etc. Our discussion provides insight for further research on epigenetics response to nutrient stress in the future.
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http://dx.doi.org/10.3390/genes13060992DOI Listing
May 2022

The Optimal Error Estimate of the Fully Discrete Locally Stabilized Finite Volume Method for the Non-Stationary Navier-Stokes Problem.

Entropy (Basel) 2022 May 30;24(6). Epub 2022 May 30.

School of Medical Information and Engineering, Southwest Medical University, Luzhou 646099, China.

This paper proves the optimal estimations of a low-order spatial-temporal fully discrete method for the non-stationary Navier-Stokes Problem. In this paper, the semi-implicit scheme based on Euler method is adopted for time discretization, while the special finite volume scheme is adopted for space discretization. Specifically, the spatial discretization adopts the traditional triangle P1-P0 trial function pair, combined with macro element form to ensure local stability. The theoretical analysis results show that under certain conditions, the full discretization proposed here has the characteristics of local stability, and we can indeed obtain the optimal theoretic and numerical order error estimation of velocity and pressure. This helps to enrich the corresponding theoretical results.
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http://dx.doi.org/10.3390/e24060768DOI Listing
May 2022

Urine Proteome in Distinguishing Hepatic Steatosis in Patients with Metabolic-Associated Fatty Liver Disease.

Diagnostics (Basel) 2022 Jun 7;12(6). Epub 2022 Jun 7.

Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.

Background: In patients with metabolic-associated fatty liver disease (MAFLD), hepatic steatosis is the first step of diagnosis, and it is a risk predictor that independently predicts insulin resistance, cardiovascular risk, and mortality. Urine biomarkers have the advantage of being less complex, with a lower dynamic range and fewer technical challenges, in comparison to blood biomarkers.

Methods: Hepatic steatosis was measured by magnetic resonance imaging (MRI), which measured the proton density fat fraction (MRI-PDFF). Mild hepatic steatosis was defined as MRI-PDFF 5-10% and severe hepatic steatosis was defined as MRI-PDFF > 10%.

Results: MAFLD patients with any kidney diseases were excluded. There were 53 proteins identified by mass spectrometry with significantly different expressions among the healthy control, mild steatosis, and severe steatosis patients. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of these significantly changed urinary molecular features correlated with the liver, resulting in the dysregulation of carbohydrate derivative/catabolic/glycosaminoglycan/metabolic processes, insulin-like growth factor receptor levels, inflammatory responses, the PI3K-Akt signaling pathway, and cholesterol metabolism. Urine alpha-1-acid glycoprotein 1 (ORM1) and ceruloplasmin showed the most significant correlation with the clinical parameters of MAFLD status, including liver fat content, fibrosis, ALT, triglycerides, glucose, HOMA-IR, and C-reactive protein. According to ELISA and western blot (30 urine samples, normalized to urine creatinine), ceruloplasmin (ROC 0.78, = 0.034) and ORM1 (ROC 0.87, = 0.005) showed moderate diagnostic accuracy in distinguishing mild steatosis from healthy controls. Ceruloplasmin (ROC 0.79, = 0.028) and ORM1 (ROC 0.81, = 0.019) also showed moderate diagnostic accuracy in distinguishing severe steatosis from mild steatosis.

Conclusions: Ceruloplasmin and ORM1 are potential biomarkers in distinguishing mild and severe steatosis in MAFLD patients.
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http://dx.doi.org/10.3390/diagnostics12061412DOI Listing
June 2022

Comparative proteome and cis-regulatory element analysis reveals specific molecular pathways conserved in dog and human brains.

Mol Cell Proteomics 2022 Jun 20:100261. Epub 2022 Jun 20.

State Key Laboratory for Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. Electronic address:

Brain development and function is governed by precisely regulated protein expressions in different regions. To date, multiregional brain proteomes have been systematically analyzed only for adult human and mouse brains. To understand the underpinnings of brain development and function, we generated proteomes from six regions of the postnatal brain at three developmental stages of domestic dogs (Canis familiaris), which are special among animals in terms of their remarkable human-like social cognitive abilities. Quantitative analysis of the spatiotemporal proteomes identified region-enriched synapse types at different developmental stages and differential myelination progression in different brain regions. Through integrative analysis of inter-regional expression patterns of orthologous proteins and genome-wide cis-regulatory element frequencies, we found that proteins related with myelination and hippocampus were highly correlated between dog and human but not between mouse and human, although mouse is phylogenetically closer to human. Moreover, the global expression patterns of neurodegenerative disease and autism spectrum disorder-associated proteins in dog brain more resemble human brains than in mouse brain. The high similarity of myelination and hippocampus-related pathways in dog and human at both proteomic and genetic levels may contribute to their shared social cognitive abilities. The inter-regional expression patterns of disease-associated proteins in the brain of different species provide important information to guide mechanistic and translational study using appropriate animal models.
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http://dx.doi.org/10.1016/j.mcpro.2022.100261DOI Listing
June 2022

Ginsenoside Rg1 Mitigates Porcine Intestinal Tight Junction Disruptions Induced by LPS through the p38 MAPK/ Inflammasome Pathway.

Toxics 2022 May 27;10(6). Epub 2022 May 27.

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510630, China.

Inflammation leads to porcine tight junction disruption of small intestinal epithelial cells, resulting in intestinal dysfunction. Herein, we established lipopolysaccharide (LPS)-induced in-vivo and in-vitro inflammatory models. The results revealed that LPS induced tight junction disruption in IPEC-J2 cells by downregulating tight-junction-related protein zonula occludens-1 (ZO-1), occludin and claudin-1 expression, while ginsenoside Rg1 rescued such inhibition and abrogated the upregulated expression of phosphorylation p38 MAPK. The p38 MAPK inhibitor (SB203580) showed a similar effect with Rg1 and attenuated the LPS-induced inhibition of ZO-1, occludin and claudin-1 expression, which is consistent with the reduced expression of inflammasome and . Furthermore, the specific inhibitors of and result in increased expression of tight-junction-related protein, demonstrating that p38 MAPK signaling was associated with Rg1 suppression of tight junction disruption. Besides, LPS treatment decreased the expression of ZO-1, occludin and claudin-1 through p38 MAPK signaling, and caused abnormal morphological changes in murine ileum. Meanwhile, Rg1 attenuated the decreased expression of ZO-1, occludin and claudin-1 and partially alleviated LPS-induced morphological changes in murine ileum. In summary, these findings characterized a novel mechanism by which Rg1 alleviates LPS-induced intestinal tight junction disruption by inhibiting the p38 MAPK-mediated inflammasome pathway.
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http://dx.doi.org/10.3390/toxics10060285DOI Listing
May 2022

Recent Advances of Biochar-Based Electrochemical Sensors and Biosensors.

Biosensors (Basel) 2022 May 31;12(6). Epub 2022 May 31.

Provincial Key Laboratory of Rural Energy Engineering in Yunnan, Yunnan Normal University, Kunming 650500, China.

In the context of accelerating the global realization of carbon peaking and carbon neutralization, biochar produced from biomass feedstock via a pyrolysis process has been more and more focused on by people from various fields. Biochar is a carbon-rich material with good properties that could be used as a carrier, a catalyst, and an absorbent. Such properties have made biochar a good candidate as a base material in the fabrication of electrochemical sensors or biosensors, like carbon nanotube and graphene. However, the study of the applications of biochar in electrochemical sensing technology is just beginning; there are still many challenges to be conquered. In order to better carry out this research, we reviewed almost all of the recent papers published in the past 5 years on biochar-based electrochemical sensors and biosensors. This review is different from the previously published review papers, in which the types of biomass feedstock, the preparation methods, and the characteristics of biochar were mainly discussed. First, the role of biochar in the fabrication of electrochemical sensors and biosensors is summarized. Then, the analytes determined by means of biochar-based electrochemical sensors and biosensors are discussed. Finally, the perspectives and challenges in applying biochar in electrochemical sensors and biosensors are provided.
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http://dx.doi.org/10.3390/bios12060377DOI Listing
May 2022

Simple synthesis of green luminescent N-doped carbon dots for malachite green determination.

Anal Methods 2022 Jun 23. Epub 2022 Jun 23.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, Shanxi, China.

In this paper, novel N-doped carbon dots (N-CDs) were prepared from fuchsin basic and ethylenediamine tetraacetic acid-disodium salt (EDTA-2Na). The N-CDs were characterized by a series of techniques and it was found that the average particle size was 2.75 nm, and the surface had functional groups such as -NH and -COOH. Interestingly, N-CDs exhibited a fast and sensitive response to malachite green (MG), which may be due to the inner filter effect (IFE). A method for the detection of MG in water samples from Jinyang Lake was developed using N-CDs, with a limit of detection (LOD) as low as 27.28 nM. Furthermore, N-CDs were utilized in the biological imaging of .
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http://dx.doi.org/10.1039/d2ay00682kDOI Listing
June 2022

Weight Status Modulated Brain Regional Homogeneity in Long-Term Male Smokers.

Front Psychiatry 2022 6;13:857479. Epub 2022 Jun 6.

Department of Magnetic Resonance Imaging, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Tobacco smoking and being overweight could lead to adverse health effects, which remain an important public health problem worldwide. Research indicates that overlapping pathophysiology may contribute to tobacco addiction and being overweight, but the neurobiological interaction mechanism between the two factors is still unclear.

Methods: The current study used a mixed sample design, including the following four groups: (i) overweight long-term smokers ( = 24); (ii) normal-weight smokers ( = 28); (iii) overweight non-smokers ( = 19), and (iv) normal-weight non-smokers ( = 28), for a total of 89 male subjects. All subjects underwent resting-state functional magnetic resonance imaging (rs-fMRI). Regional homogeneity (ReHo) was used to compare internal cerebral activity among the four groups. Interaction effects between tobacco addiction and weight status on ReHo were detected using a two-way analysis of variance, correcting for age, years of education, and head motion.

Results: A significant interaction effect between tobacco addiction and weight status is shown in right superior frontal gyrus. Correlation analyses show that the strengthened ReHo value in the right superior frontal gyrus is positively associated with pack-year. Besides, the main effect of tobacco addiction is specially observed in the occipital lobe and cerebellum posterior lobe. As for the main effect of weight status, the right lentiform nucleus, left postcentral gyrus, and brain regions involved in default mode network (DMN) survived.

Conclusions: These results shed light on an antagonistic interaction on brain ReHo between tobacco addiction and weight status in the right superior frontal gyrus, which may be a clinical neuro-marker of comorbid tobacco addiction and overweight. Our findings may provide a potential target to develop effective treatments for the unique population of comorbid tobacco addiction and overweight people.
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http://dx.doi.org/10.3389/fpsyt.2022.857479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207237PMC
June 2022

Two distinct subtypes of obsessive compulsive disorder revealed by a framework integrating multimodal neuroimaging information.

Hum Brain Mapp 2022 Jun 21. Epub 2022 Jun 21.

Department of Magnetic Resonance Imaging, the First Affiliated Hospital of Zhengzhou University, China.

Patients with obsessive compulsive disorder (OCD) exhibit tremendous heterogeneity in structural and functional neuroimaging aberrance. However, most previous studies just focus on group-level aberrance of a single modality ignoring heterogeneity and multimodal features. On that account, we aimed to uncover OCD subtypes integrating structural and functional neuroimaging features with the help of a multiview learning method and examined multimodal aberrance for each subtype. Ninety-nine first-episode untreated patients with OCD and 104 matched healthy controls (HCs) undergoing structural and functional MRI were included in this study. Voxel-based morphometric and amplitude of low-frequency fluctuation (ALFF) were adopted to assess gray matter volumes (GMVs) and the spontaneous neuronal fluctuations respectively. Structural/functional distance network was obtained by calculating Euclidean distance between pairs of regional GMVs/ALFF values across patients. Similarity network fusion, one of multiview learning methods capturing shared and complementary information from multimodal data sources, was used to fuse multimodal distance networks into one fused network. Then spectral clustering was adopted to categorize patients into subtypes. As a result, two robust subtypes were identified. These two subtypes presented opposite GMV aberrance and distinct ALFF aberrance compared with HCs while shared indistinguishable clinical and demographic features. In addition, these two subtypes exhibited opposite structure-function difference correlation reflecting distinct adaptive modifications between multimodal aberrance. Altogether, these results uncover two objective subtypes with distinct multimodal aberrance and provide a new insight into taxonomy of OCD.
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http://dx.doi.org/10.1002/hbm.25951DOI Listing
June 2022

Microscopic Replantation of Penile Glans Amputation Due to Circumcision.

J Vis Exp 2022 Jun 3(184). Epub 2022 Jun 3.

Department of Urology, Daping Hospital, Army Medical University;

Circumcision using a disposable stapler is becoming quite popular in China. However, improper surgical procedures also bring the risk of penile glans amputation, which is a very rare iatrogenic genital injury. Such complication is conventionally treated by simple hemostasis to achieve self-healing, early gross replantation, or delayed plastic surgery. However, these may lead to obvious unfavorable outcomes such as amputated glans loss, necrosis, malformation healing, or urethral orifice stenosis. In the present study, we adopted microscopic replantation as an emergency approach to achieve the precise anastomoses and anatomic reconstruction of penile glans. The goal of this protocol is to present a detailed emergency management strategy with meticulous surgical skills for the penile glans amputation. The postoperative results showed that the original shape of the glans was perfectly restored with satisfactory cosmetic appearance. The micturition function was completely restored to normal without any obvious complications. There was also no significant reduction in the sensation of amputated glans area. Hence, early meticulous microscopic replantation as soon as possible is an ideal emergency management strategy for the penile glans amputation due to circumcision.
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http://dx.doi.org/10.3791/63691DOI Listing
June 2022

Exploring the Action Mechanism of the Active Ingredient of Quercetin in on the Mouse Mastitis Model Based on Network Pharmacology and Molecular Biology Validation.

Evid Based Complement Alternat Med 2022 10;2022:4236222. Epub 2022 Jun 10.

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.

Aim: The aim of this study is to explore the mechanism of action of quercetin, the main active anti-inflammatory component of , in the prevention and treatment of mastitis.

Methods: Prediction of the main active ingredients and key anti-inflammatory targets of using a network pharmacology platform and molecular biology validation of the results. Observation of histopathological changes in the mouse mammary gland by hematoxylin-eosin staining(H&E) method, quantitative real-time PCR(qPCR), and Western blot (WB) to detect the expression levels of relevant inflammatory factors mRNA and protein.

Results: A total of 7 active ingredients and 42 key targets were obtained from the network pharmacological analysis of , with quercetin as the main core ingredient and tumor necrosis factor(TNF), serine threonine protein kinase1(AKT1), and interleukin6(IL6) as the core targets; H&E results showed that pathological changes were reduced to different degrees in the dose group compared to the model group. The qPCR results showed that the relative expression of and mRNA in the high dose group on day 3 and the high and medium dose groups on day 7 were not significantly different compared with the blank group ( > 0.05), and the difference between the dose groups on day 5 was significant ( < 0.05). WB results showed that the difference in nuclear factor kappa-B(NF-B) protein expression in the medium and low dose groups on day 7 was significant compared with the blank group ( < 0.05), the difference in 5 and 7 days, significant differences in AKT1 protein expression between the middle and low dose groups ( < 0.05), nonsignificant differences in the TNF protein expression between the high dose groups on day 7 ( > 0.05), and significant differences in the IL6 protein expression between the middle and low dose groups on days 3 and 7 ( < 0.05).

Conclusion: Quercetin, the main active ingredient of , may act in the prevention and treatment of mastitis by inhibiting the expression of inflammatory factors in phosphoinositol 3-kinase(PI3K)-AKT and NF-B signaling pathways and showa a significant dose-dependent effect. This study provides theoretical basis and clues for the control of mastitis in dairy cows.
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http://dx.doi.org/10.1155/2022/4236222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205729PMC
June 2022

Enhancing the Phytoremediation of Heavy Metals by Combining Hyperaccumulator and Heavy Metal-Resistant Plant Growth-Promoting Bacteria.

Front Plant Sci 2022 2;13:912350. Epub 2022 Jun 2.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Heavy metals (HMs) have become a major environmental pollutant threatening ecosystems and human health. Although hyperaccumulators provide a viable alternative for the bioremediation of HMs, the potential of phytoremediation is often limited by the small biomass and slow growth rate of hyperaccumulators and HM toxicity to plants. Here, plant growth-promoting bacteria (PGPB)-assisted phytoremediation was used to enhance the phytoremediation of HM-contaminated soils. A PGPB with HM-tolerant (HMT-PGPB), sp. PGP15 was isolated from the rhizosphere of a cadmium (Cd) hyperaccumulator, . Pot experiments demonstrated that inoculation with strain PGP15 could significantly increase the growth of . More importantly, strain PGP15 markedly improved Cd accumulation in while alleviating Cd-induced stress in . Specifically, PGP15 inoculation significantly decreased the contents of HO, MDA, and in , while the activities (per gram plant fresh weight) of SOD, APX, and CAT were significantly increased in the PGP15-inoculated plants compared with the control sample. These results suggested that the interactions between strain PGP15 and could overcome the limits of phytoremediation alone and highlighted the promising application potential of the PGPB-hyperaccumulator collaborative pattern in the bioremediation of HM-contaminated soils. Furthermore, the PGP15 genome was sequenced and compared with other strains to explore the mechanisms underlying plant growth promotion by HMT-PGPB. The results showed that core genes that define the fundamental metabolic capabilities of strain PGP15 might not be necessary for plant growth promotion. Meanwhile, PGP15-specific genes, including many transposable elements, played a crucial role in the adaptive evolution of HM resistance. Overall, our results improve the understanding of interactions between HMT-PGPB and plants and facilitate the application of HMT-PGPB in the phytoremediation of HM-contaminated soils.
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http://dx.doi.org/10.3389/fpls.2022.912350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201774PMC
June 2022

Neoadjuvant Pembrolizumab and Chemotherapy in Resectable Esophageal Cancer: An Open-Label, Single-Arm Study (PEN-ICE).

Front Immunol 2022 2;13:849984. Epub 2022 Jun 2.

Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University, Xi'an, China.

Background: In this single-arm study, the efficacy and safety of neoadjuvant pembrolizumab plus chemotherapy were evaluated in patients with resectable esophageal squamous cell carcinoma (ESCC).

Methods: This study included patients with ESCC of clinical stages II-IVA who underwent surgery within 4 to 6 weeks after completing treatment with pembrolizumab (200 mg) combined with a conventional chemotherapy regimen (3 cycles). The safety and efficacy of this combination treatment were evaluated as primary endpoints of the study.

Results: From April 2019 to August 2020, a total of 18 patients (including 14 men) were enrolled, of whom 13 patients progressed to surgery. Postoperative pathology revealed a major pathological response (MPR) in 9 cases (9/13, 69.2%) and a pathological complete response (pCR) in 6 cases (6/13, 46.2%). Five patients (5/18, 27.8%) experienced serious treatment-related adverse events (AEs) of grades 3-4. At the time of data cutoff (Mar 25, 2022), the shortest duration of follow-up was 17.8 months. Programmed death-ligand 1 (PD-L1) expression in pretreatment specimens was not significantly associated with the percentage of residual viable tumor (RVT) (r=-0.55, P=0.08). Changes in counts of CD68 macrophage between pre- and post-treatment specimens were weakly correlated with RVT (r=0.71; P=0.07), while a positive correlation was observed between postoperative forkhead box P3-positive (Foxp3)T cells/CD4Tcells ratios and RVT (r=0.84, P0.03).

Conclusions: The combination of neoadjuvant immunotherapy and chemotherapy for ESCC is associated with a high pathological response and immunologic effects in the tumor microenvironment (TME). It has acceptable toxicity and great efficacy, suggesting a strong rationale for its further evaluation in randomized clinical trials (RCTs).

Trial Registration: ChiCTR2100048917.
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http://dx.doi.org/10.3389/fimmu.2022.849984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202755PMC
June 2022

Altered Effective Connectivity of Resting-State Networks by Tai Chi Chuan in Chronic Fatigue Syndrome Patients: A Multivariate Granger Causality Study.

Front Neurol 2022 3;13:858833. Epub 2022 Jun 3.

Department of Rehabilitation, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Numerous evidence has shown that patients with chronic fatigue syndrome (CFS) have changes in resting brain functional connectivity, but there is no study on the brain network effect of Tai Chi Chuan intervention in CFS. To explore the influence of Tai Chi Chuan exercise on the causal relationship between brain functional networks in patients with CFS, 21 patients with CFS and 19 healthy controls were recruited for resting-state functional magnetic resonance imaging (rs-fMRI) scanning and 36-item Short-Form Health Survey (SF-36) scale assessment before and after 1month-long training in Tai Chi Chuan. We extracted the resting brain networks using the independent component analysis (ICA) method, analyzed the changes of FC in these networks, conducted Granger causality analysis (GCA) on it, and analyzed the correlation between the difference causality value and the SF-36 scale. Compared to the healthy control group, the SF-36 scale scores of patients with CFS were lower at baseline. Meanwhile, the causal relationship between sensorimotor network (SMN) and default mode network (DMN) was weakened. The above abnormalities could be improved by Tai Chi Chuan training for 1 month. In addition, the correlation analyses showed that the causal relationship between SMN and DMN was positively correlated with the scores of Role Physical (RP) and Bodily Pain (BP) in CFS patients, and the change of causal relationship between SMN and DMN before and after training was positively correlated with the change of BP score. The findings suggest that Tai Chi Chuan is helpful to improve the quality of life for patients with CFS. The change of Granger causality between SMN and DMN may be a readout parameter of CFS. Tai Chi Chuan may promote the functional plasticity of brain networks in patients with CFS by regulating the information transmission between them.
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http://dx.doi.org/10.3389/fneur.2022.858833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203735PMC
June 2022

COVID-19 Pandemic Impact on Cardiometabolic Markers in Adults in Chongqing, China: A Retrospective Cohort Study.

Front Public Health 2022 3;10:859488. Epub 2022 Jun 3.

Department of Health Management, The Second Hospital Affiliated With Chongqing Medical University, Chongqing, China.

The influx of COVID-19 infection and government-enforced lockdowns and social isolation changed people's lifestyles. Concerns regarding the health impact of the COVID-19 pandemic due to the new sedentary lifestyle. This study aims to investigate the impact of the COVID-19 pandemic on cardiovascular health factors. A retrospective observational study was conducted using historical medical records. The cohort consisted of healthy adults (without chronic non-communicable diseases) over 18 years of age who have undertaken a health examination at the Chongqing Medical University from 2019 to 2020. The analysis of covariance (ANCOVA) test was used to compare variables between 2019 and 2020. The effect of exposure time to COVID-19 on cardiometabolic markers was analyzed using multiple linear regression models. 29,773 participants took part in this study. The average age was 42.5 ± 13.44 years at baseline, and the average follow-up period was 12.7 ± 2.8 months. Analysis showed that weight, BMI, waist circumference, hip circumference, WHR, fasting blood glucose, TG, LDL, uric acid, and liver enzymes increased significantly during the COVID-19 pandemic ( < 0.05). This study showed evidence that the COVID-19 pandemic and its control measures negatively impacted cardiometabolic profiles.
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http://dx.doi.org/10.3389/fpubh.2022.859488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204008PMC
June 2022

[Distribution characteristics of 46 elements in drinking water in Ankang area in 2020].

Wei Sheng Yan Jiu 2022 May;51(3):437-462

Beijing Center for Disease Prevention and Control, Beijing 100013, China School of Public Health, Capital Medical University, Beijing 100069, China.

Objective: To understand the distribution characteristics of 46 elements in drinking water in Ankang area of Shanxi Province.

Methods: A total of 46 elements in drinking water samples collected in Ankang area during dry and wet seasons in 2020 were determined by inductively coupled plasma mass spectrometry(ICP-MS). According to the "drinking water hygiene standards"(GB 5749-2022) and "food safety national standards". the 46 elements were classified As general chemical indexes(Al, Mn, Fe, Cu, Zn), toxicological indexes(Cr, As, Cd, Hg, Pb, Ba, B, Mo, Ni, Sb, Be, Ag, Tl), new reference indexes(U, V), and major elements(K, Ca, N) A, Mg), trace elements(Li, Co, Se, Sr, Sn) and rare earth elements(Sc, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Th) were analyzed and described.

Results: The maximum values of Al and Fe in drinking water in Ankang area were 1.21 and 0.98 mg/L, exceeding the limits of 0.2 and 0.3 mg/L. In dry season, the median content of Fe in drinking water of different water sources was higher in groundwater than in surface water. The Al and Fe of surface water were higher than that of groundwater in wet season. The toxicological indexes all met the standard requirements, and there was no significant difference among districts and counties. The median content of Na in drinking water of different water sources was higher in groundwater than in surface water, while Mg, K and Ca were higher in surface water than in groundwater. The maximum value of the newly added reference index U was 0.015 mg/L lower than the limit standard 0.03 mg/L, and the maximum value of V was 0.019 mg/L higher than the limit standard 0.01 mg/L, but the median of both indexes were low. The median content of Li in drinking water of different water sources was that surface water was higher than groundwater, while Se and Sr were higher in groundwater than surface water. The maximum content of Se was 0.016 mg/L in Ziyang County, and Sr content was generally higher. The content of rare earth elements was low, mostly below the detection.

Conclusion: The drinking water quality in Ankang area is excellent and rich in strontium. However, the general chemical indexes Al, Fe and the newly added appendix index V in some sampling points exceed the limit standard, so supervision and monitoring should be strengthened.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.016DOI Listing
May 2022

Mast cell leukemia with novel BRAF variant and concomitant atypical KIT variant.

Cancer Genet 2022 May 24;266-267:33-36. Epub 2022 May 24.

Department of Pathology, Penn State Hershey Medical Center, Hershey, PA, United States of America.

Mast cell leukemia (MCL) is a leukemic variant of systemic mastocytosis defined by mast cells ≥ 20% of marrow nucleated cells. Its incidence is < 1% of all systemic mastocytosis cases [1]. Clinical characteristics and treatment of the disease are not well established and overall prognosis is very poor. We report a case of de novo mast cell leukemia with novel BRAF variant, concomitant KIT exon 9 missense mutation and complex cytogenetic abnormalities. After careful review of the literature we have not found any prior reports of concomitant BRAF and KIT variants, and complex cytogenetic abnormalities in MCL. This case provides evidence that MCL can have wide spectrum of genetic abnormalities as well as accumulation of mutations in various genes including BRAF. This finding may have significant implications for the understanding of pathogenesis, diagnosis, as well as targeted therapy of MCL.
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http://dx.doi.org/10.1016/j.cancergen.2022.05.040DOI Listing
May 2022

Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.

J Biol Chem 2022 Jun 14:102142. Epub 2022 Jun 14.

Department of Molecular Biology and Genetics, Universitetsbyen 81, Aarhus University, DK-8000 Aarhus C, Denmark. Electronic address:

The bacterial stringent response involves wide-ranging metabolic reprogramming aimed at increasing long-term survivability during stress conditions. One of the hallmarks of the stringent response is the production of a set of modified nucleotides, known as alarmones, which affect a multitude of cellular pathways in diverse ways. Production and degradation of these molecules depend on the activity of enzymes from the RelA/SpoT homologous (RSH) family, which come in both bifunctional (containing domains to both synthesize and hydrolyze alarmones) and monofunctional (consisting of only synthetase or hydrolase domain) variants, of which the structure, activity, and regulation of the bifunctional RSHs have been studied most intensely. Despite playing an important role in guanosine nucleotide homeostasis in particular, mechanisms of regulation of the small alarmone hydrolases (SAH) are still rather unclear. Here, we present crystal structures of SAH enzymes from Corynebacterium glutamicum (RelH) and Leptospira levettii (RelH) and show that while being highly similar, structural differences in substrate access and dimer conformations might be important for regulating their activity. We propose that a varied dimer form is a general property of the SAH family, based on current structural information as well as prediction models for this class of enzymes. Finally, subtle structural variations between monofunctional and bifunctional enzymes point to how these different classes of enzymes are regulated.
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http://dx.doi.org/10.1016/j.jbc.2022.102142DOI Listing
June 2022

Colorectal Cancer Screening With High Risk-Factor Questionnaire and Fecal Immunochemical Tests Among 5, 947, 986 Asymptomatic Population: A Population-Based Study.

Front Oncol 2022 30;12:893183. Epub 2022 May 30.

Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, China.

Background: The recent uptrend in colorectal cancer (CRC) incidence in China is causing an increasingly overwhelming social burden. And its occurrence can be effectively reduced by sensitizing CRC screening for early diagnosis and treatment. However, a large number of people in China do not undergo screening due to multiple factors. To address this issue, since 2012, a CRC screening program has been initiated in Tianjin.

Methods: Residents aged 40-74 years were eligible for CRC screening. The first was to complete the high-risk factor questionnaire (HRFQ) and undergo fecal immunochemical test (FIT). Then those with a positive result in any of the two screening methods were recommended for a free colonoscopy.

Results: The detection rate of intestinal diseases increased with age, had a male predominance, and was higher in residents from central urban areas and those with primary school above education level. The sensitivity of predicting CRC after colonoscopy in the high-risk group was 76.02%; the specificity was 25.33%.A significant decrease in the detection rate of intestinal disease, CRC and advanced adenoma was observed from positive FIT, the high-risk group and positive HRFQ, 47.13%, 44.79%, 42.30%; 3.15%, 2.44%, 1.76%; 7.72%, 6.42%, 5.08%, in that order, while no inter-group difference was found for the detection of polyps. In addition, the different combinations of HRFQ and FIT can enroll more high-risk population than FIT or (and) HRFQ only, and thus detect more intestinal diseases (include CRC/AA/Polyp).

Conclusion: The superimposition of different screening method for HRFQ and FIT is an effective strategy for the detection of CRC, AA, and Polyp, compared to HRFQ or FIT alone. However, further improvements in screening and interventions are needed to promote colonoscopy compliance.
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http://dx.doi.org/10.3389/fonc.2022.893183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195590PMC
May 2022

Distribution of extracellular matrix related proteins in normal and cryptorchid ziwuling black goat testes.

Anim Reprod 2022 1;19(2):e20220005. Epub 2022 Jun 1.

Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, College of Veterinary Medicine, Gansu Agricultural University, Lan Zhou, Gansu Province, China.

The Ziwuling black goat is an indigenously in China, their offspring are frequently affected by congenital cryptorchidism. The extracellular matrix (ECM) contains cytokines and growth factors that regulate the development of the testis, and component changes often result in pathological changes. Cryptorchidism is closely related to structural changes in ECM. In this study, the histochemical staining, immunohistochemical, immunofluorescence and Western blot combined with semi-quantitative analysis was used to describe the distribution of the important ECM components Collagen type IV (Col IV), laminin (LN)and heparan sulfate proteoglycans (HSPG) in the normal and cryptorchid testes of Ziwuling black goats. Results showed that: The histochemical staining showed that the dysplasia of seminiferous tubules and decreased number of Sertoli cells in cryptorchidism, as well as sparse collagen fiber. Meanwhile, the distribution of reticular fibers is relatively rich. Furthermore, the PAS and AB staining in the interstitial vessels and lamina propria of seminiferous tubules is weak. The immunohistochemical and immunofluorescence revealed that Col IV, LN was strongly expressed in Leydig, Sertoli cells of normal testes and moderately positive in the spermatogonia and spermatids, but HSPG was not expressed in the spermatogonia. However, cryptorchidism, the expression of Col IV, LN and HPSG in Leydig, Sertoli cells significantly decreased, as well as the expression of Col IV and LN in capillary endothelial cells, but HSPG was moderately expressed in spermatogonia. Based on these data, the underdevelopment of spermatogenic epithelium, decreased synthesis function of collagen fibers and Leydig cells develop usually in the cryptorchidism were shown to be closely related to the abnormal metabolism of Col IV and LN. The positive expressed of HSPG in the spermatogonia of cryptorchid testes is related to the compensatory development of spermatogonia.
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http://dx.doi.org/10.1590/1984-3143-AR2022-0005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170007PMC
June 2022

Metagenomic Analysis Revealed Differences in Composition and Function Between Liquid-Associated and Solid-Associated Microorganisms of Sheep Rumen.

Front Microbiol 2022 27;13:851567. Epub 2022 May 27.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

The rumen microbiota plays a key role in the utilization of plant materials by ruminants, yet little is known about the key taxa and their genetic functions of the rumen sub-environment involved in the ruminal degradation process. Understanding the differences in the composition and function of ruminal microbiota in the liquid-associated (LA) and solid-associated (SA) systems is needed to further study and regulate rumen function and health. In this study, rumen contents of nine sheep were collected to separate LA and SA systems with elution and centrifugal precipitation. Metagenome sequencing was used to investigate the differences in microbial composition and genetic functions of LA and SA systems, with special emphasis on their degradational potential toward carbohydrates. Results showed that the dominant species composition was similar between the two systems, but SA microorganisms had a higher relative abundance than LA microorganisms in all taxa. The concentration of fiber-degrading bacteria, such as , and , was higher and was lower in the SA vs. LA system. Additionally, SA microorganisms dominated in cellulose degradation, while LA microorganisms were more important in starch utilization based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO)'s functional categories and Carbohydrate-Active Enzymes (CAZymes). In general, SA microorganisms are more abundant and important in metabolic functions than LA, such as carbohydrate and amino acid metabolisms. In summary, the key differential biomarkers between LA and SA systems were , and . Ruminal microbes degraded carbohydrates synergistically with SA, thus, more focusing on cellulose and hemicellulose, while LA is more important to starch.
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http://dx.doi.org/10.3389/fmicb.2022.851567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197192PMC
May 2022

Sustainable power generation for at least one month from ambient humidity using unique nanofluidic diode.

Nat Commun 2022 Jun 16;13(1):3484. Epub 2022 Jun 16.

Tribology Research Institute, School of Mechanical Engineering, Southwest Jiaotong University, Chengdu, 610031, PR China.

The continuous energy-harvesting in moisture environment is attractive for the development of clean energy source. Controlling the transport of ionized mobile charge in intelligent nanoporous membrane systems is a promising strategy to develop the moisture-enabled electric generator. However, existing designs still suffer from low output power density. Moreover, these devices can only produce short-term (mostly a few seconds or a few hours, rarely for a few days) voltage and current output in the ambient environment. Here, we show an ionic diode-type hybrid membrane capable of continuously generating energy in the ambient environment. The built-in electric field of the nanofluidic diode-type PN junction helps the selective ions separation and the steady-state one-way ion charge transfer. This directional ion migration is further converted to electron transportation at the surface of electrodes via oxidation-reduction reaction and charge adsorption, thus resulting in a continuous voltage and current with high energy conversion efficiency.
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http://dx.doi.org/10.1038/s41467-022-31067-zDOI Listing
June 2022

Implementation of sodium alginate-FeO to localize undiagnosed small pulmonary nodules for surgical management in a preclinical rabbit model.

Sci Rep 2022 Jun 15;12(1):9979. Epub 2022 Jun 15.

Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.

Many methods are used to locate preoperative small pulmonary nodules. However, deficiencies of complications and success rates exist. We introduce a novel magnetic gel for small pulmonary nodules localization in rabbit model, and furtherly evaluate its safety and feasibility. Rabbits were used as the experimental objects. A magnetic gel was used as a tracer magnet, mixed as sodium alginate-FeO magnetic fluid and calcium gluconate solution. In short-term localization, a coaxial double-cavity puncture needle was applied to inject the gel into the lung after thoracotomy, and a pursuit magnet made of Nd-Fe-B permanent magnetic materials was used to attract the gel representing location of the nodule. In long-term localization, the gel was injected under X-ray guidance. Imaging changes to the lung were observed under X-ray daily. Thoracotomy was performed to excise tissue containing the gel, and hematoxylin-eosin staining was used to observe the tissue on postoperative days 1, 3, 5, and 7. Observe tissues morphology of heart, liver, spleen, and kidney in the same way. The gel was formed after injection and drew lung tissue to form a protrusion from the lung surface under the applied magnetic field. No complication was observed. The shape and position of the gel had not changed when viewed under X-ray. Pathological analysis showed the gel had a clear boundary without diffusion of magnetic fluid. All tissues retained good histologic morphology and no magnetic fluid was observed. Our study preliminarily suggested that the technique using sodium alginate-FeO magnetic gel to locate small pulmonary nodules with guidance of X-ray, and to search for them under an applied magnetic field during the operation is safe and feasible.
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http://dx.doi.org/10.1038/s41598-022-13884-wDOI Listing
June 2022

Warming and spring precipitation addition change plant growth pattern but have minor effects on growing season mean gross ecosystem productivity in an alpine meadow.

Sci Total Environ 2022 Jun 13;841:156712. Epub 2022 Jun 13.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China; National Agricultural Experimental Station for Agricultural Environment, Nagqu, China. Electronic address:

Gross ecosystem productivity (GEP) plays an important role in global carbon cycling. However, how plant phenology and growth rate regulate GEP under climate change is unclear. Based on an in situ manipulative experiment using open top chambers from 2015 to 2018, we measured whole year warming and spring precipitation addition effects on plant phenology, plant growth rate and GEP. Our results showed that warming delayed plant green up (4 days) and withering (5 days), while spring precipitation addition advanced green up 13 days and did not change withering. Warming delayed the timing of the fast-growing phase 7 days, shortened length of the fast-growing phase 7 days and marginally increased the growth rate. Spring precipitation addition advanced the timing of the fast-growing phase 6 days, but did not change the length of the fast-growing phase or the growth rate. Both whole year warming and spring precipitation addition have not significantly affected growing season mean GEP. GEP is positively correlated with plant growth rate and negatively correlated with the length of the fast-growing phase. We provide an evidence that although warming did not change growing season mean productivity, it delayed plant fast-growing phase. Our findings suggest that management approaches for increasing water availability before the fast-growing phase should be intensified to increase ecosystem carbon uptake and grass supply for animal husbandry in spring.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156712DOI Listing
June 2022

Supramolecular confinement pyrolysis to carbon-supported Mo nanostructures spanning four scales for hydroquinone determination.

J Hazard Mater 2022 Jun 9;437:129327. Epub 2022 Jun 9.

College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address:

Metal nanostructures with high atom utilization, abundant active sites, and special electron structures should be beneficial to the electrochemical monitoring of hydroquinone (HQ), a highly toxic environmental pollutant. However, traditional nanostructures, especially non-noble metals generally suffer from severe aggregation, or consist of a mixture of nanoparticles and nanoclusters, resulting in low detection sensitivity. Herein, we precisely control the size of Mo-based nanostructures spanning four scales (viz. MoC nanoparticles, MoC nanodots, Mo nanoclusters and Mo single atoms) anchored on N, P, O co-doped carbon support. The detection sensitivity of four samples toward the HQ follows the orders of Mo single atoms>MoC nanodots>Mo nanoclusters>MoC nanoparticles. The catalytic ability of four catalysts is investigated, also showing the same order. The supported Mo single atoms show superior electro-sensing performance for HQ with wide linear range (0.02-200 μM) and low detection limit (0.005 μM), surpassing most previously reported catalysts. Moreover, the coexistence of dihydroxybenzene isomers of catechol (CC) and resorcinol (RC) does not interfere with the detection of HQ on the Mo single-atom sensor. This work opens up a polyoxometalate-based confinement pyrolysis approach to constructing ultrafine metal-based nanostructures spanning multiple-scales for efficient electrochemical applications.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129327DOI Listing
June 2022
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