Publications by authors named "Yong Yang"

2,172 Publications

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Source identification and comprehensive apportionment of the accumulation of soil heavy metals by integrating pollution landscapes, pathways, and receptors.

Sci Total Environ 2021 Apr 30;786:147436. Epub 2021 Apr 30.

College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of the Yangtze River), Ministry of Agriculture, China; Hubei Key Laboratory of Soil Environment and Pollution Remediation, Wuhan 430070, China.

Existing source apportionment methods for soil heavy metals fail to identify the actual landscapes related to pollutant sources and quantify their contributions to the accumulation of soil heavy metals. In this work, we propose a new source identification and apportionment approach for soil heavy metal accumulation by integrating pollution landscapes, pathways, and receptors. Datasets for soil lead (Pb) concentrations in Daye city, China, which was sampled in 2018, were used. First, based on the spatial distribution of Pb, the source landscapes were identified using GeoDetector and spatial analysis methods. Second, a source landscape apportionment model (SLAM) was developed considering both atmospheric deposition and surface runoff as diffusion pathways. Third, considering soil properties and topography as receptor attributes, ordinary least squares (OLS) and geographically weighted regression (GWR) models were employed to further adjust the soil Pb accumulation at receptor locations. The results showed that SLAM followed by the GWR model (SLAM-GWR) had the highest fitting accuracy. Then, the spatial distributions and ranges of contributions of each identified source landscape to Pb accumulation through different pathways were obtained. Finally, the advantages and disadvantages of the proposed approach were discussed.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147436DOI Listing
April 2021

lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis.

Mol Med Rep 2021 Jul 13;24(1). Epub 2021 May 13.

Department of Gastroenterology, Zhongda Hospital Southeast University, Medical College of Southeast University, Nanjing, Jiangsu 210000, P.R. China.

Gastric cancer is a serious malignant tumor. Despite progression in gastric cancer research in recent years, the specific molecular mechanism underlying the pathogenesis of the disease is not completely understood. Long non‑coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) affects the proliferation and metastasis of multiple types of tumor cells in colorectal cancer and breast cancer but its specific role in gastric cancer requires further investigation. The aim of the present study was to analyze the role of NEAT1 in gastric cancer. The expression of endoplasmic reticulum stress marker proteins and apoptosis‑related proteins in gastric cancer tissue and cell lines was analyzed using western blotting. The targeting relationship of NEAT1 and miR‑500a‑3p was analyzed using dual‑luciferase reporter assay. Cell proliferation was analyzed using CCK8 assay and colony formation assay while cell invasion was detected using Transwell assay. Cell apoptosis was analyzed using TUNEL staining and LC3 expression through immunofluorescent staining (IF). The results showed that lncRNA NEAT1‑overexpression gastric cancer cells were established to determine its effects on cell proliferation, invasion, apoptosis, autophagy and endoplasmic reticulum stress. Subsequently, microRNA (miR)‑500a was overexpressed in lncRNA NEAT1‑overexpression cells. Compared with the vector group, lncRNA NEAT1 overexpression significantly inhibited gastric cancer cell proliferation and invasion, but significantly promoted cell apoptosis. Furthermore, the results indicated that lncRNA NEAT1 targeted and downregulated the expression of miR‑500a‑3p, and miR‑500a‑3p targeted X‑box binding protein‑1 (XBP‑1) mRNA. lncRNA NEAT1 overexpression‑mediated inhibition of gastric cancer cell proliferation and invasion was significantly reversed by miR‑500a‑3p overexpression. Furthermore, compared with the vector group, the expression levels of endoplasmic reticulum stress‑related proteins (XBP‑1S/XBP‑1U ratio and 78‑kDa glucose‑regulated protein) and apoptosis‑related proteins (Bax and cleaved‑caspase‑3) were significantly upregulated by lncRNA NEAT1 overexpression; however, miR‑500a‑3p overexpression reversed lncRNA NEAT1 overexpression‑mediated effects on protein expression. The present study demonstrated that lncRNA NEAT1 inhibited gastric cancer cell proliferation and invasion, and promoted apoptosis by regulating the miR‑500a‑3p/XBP‑1 axis.
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http://dx.doi.org/10.3892/mmr.2021.12142DOI Listing
July 2021

IL-6 deficiency promotes colitis by recruiting Ly6C monocytes into inflamed colon tissues in a CCL2-CCR2-dependent manner.

Eur J Pharmacol 2021 May 9:174165. Epub 2021 May 9.

Center for New Drug Safety Evaluation and Research, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu, 211198, PR China. Electronic address:

Interleukin 6 (IL-6) is a pleiotropic cytokine that is elevated in inflammatory bowel disease. However, the role of IL-6 deficiency in colitis is not well-defined. Some IL-6 and IL-6 receptor antagonists are associated with severe gastrointestinal immune adverse effects, but the mechanisms of the effects are not clear. This study aimed to investigate the effect of IL-6 in ulcerative colitis in Il6 mice. Results indicated that physiological deficiency of IL-6 promoted the development of colitis. Moreover, IL-6 deficiency significantly increased the mRNA levels of monocytes chemokine Ccl2 and its receptor Ccr2 in colon tissues. Similarly, the percentage of Ly6C monocytes and neutrophils were increased in the colon of Il6 mice. Intestinal crypts more strongly increased the migration of Il6 macrophages than wild-type ones. Moreover, Il6 macrophages promoted the migration of neutrophils. Most importantly, RS102895, an antagonist of CCR2, diminished chemotaxis of macrophages and inhibited colitis in Il6 mice. Collectively, these results indicate that Il6 macrophages migrate to inflamed colon tissues and recruit neutrophils, thereby promoting the effect of Il6 on colitis. This study expands our understanding on the effect of IL-6 deficiency in colitis and the development of gastrointestinal immune adverse effects.
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http://dx.doi.org/10.1016/j.ejphar.2021.174165DOI Listing
May 2021

Identification of as the Key Gene Associated with Antivascular Endothelial Growth Factor Therapy in Glioblastoma Multiforme.

Genet Test Mol Biomarkers 2021 May 10. Epub 2021 May 10.

Department of Neurosurgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Vascular endothelial growth factors (VEGFs) are important for glioblastoma multiforme (GBM) growth and development. However, the effects of VEGF-targeting drugs in primary GBM remain poorly understood. We aimed to explore the key genes correlated with VEGF expression and prognosis and elucidate their potential implications in GBM anti-VEGF therapy. RNA-seq data with the corresponding clinicopathological information was retrieved from The Cancer Genome Atlas and the Chinese Glioma Genome Atlas. Weighted gene coexpression network analyses was performed on differentially expressed genes to construct coexpression modules and investigate their correlation with VEGFs. Functional enrichment analyses were performed based on the coexpressed genes from the most promising modules. CytoHubba and Kaplan-Meier analyses were implemented to identify the key genes in the modules of interest. The oncomine database, quantitative reverse transcription PCR, and the Human Protein Atlas were used to investigate the expression characteristics of the identified key genes. Four modules (cyan, green, purple, and tan) correlated significantly with VEGF expression. Enrichment analyses suggested that extracellular matrix-receptor interaction, growth factor binding, and the PI3K-Akt pathways were involved in VEGF expression. Four hub genes (, , , ) associated with VEGF member were identified. Among them, was regarded as the key gene associated with anti-VEGF therapy. Further, was upregulated in GBM compared to that in normal brain tissues. overexpression predicted a worse prognosis. was identified as the key gene associated with anti-VEGF therapy and may provide novel insight into GBM targeted therapy.
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http://dx.doi.org/10.1089/gtmb.2020.0279DOI Listing
May 2021

O3-Type NaCrO as a Superior Cathode Material for Sodium/Potassium-Ion Batteries Ensured by High Structural Reversibility.

ACS Appl Mater Interfaces 2021 May 10. Epub 2021 May 10.

State Key Laboratory for Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

O3-type NaCrO is attracting increasing attention as potential cathode material for sodium-ion batteries (SIBs). Bare NaCrO is usually synthesized by a solid-state reaction and suffers from serious capacity decay and poor power capability. Modification by coating is an effective method to improve the electrochemical properties, but it inevitably reduces the energy density. To avoid the decrease of energy density and optimize the electrochemical performance, a specific route, i.e., a freeze-drying-assisted sol-gel method, has been adopted to synthesize bare NaCrO in this work. Three-phase coexistence during charging is confirmed for the first time, which contributes to delaying the disappearance of the O3 phase and then improving the structural reversibility, resulting in superior cycle stability (∼50% capacity retention after 3000 cycles at 5C). Meanwhile, as-synthesized NaCrO delivers an outstanding rate capability (82.1 mAh g at 50C), which is attributed to the fast Na diffusivity and high electronic conductivity proved by density functional theory (DFT) calculations. It is worth mentioning that NaCrO also exhibits excellent electrochemical properties when used as a cathode for potassium-ion batteries (PIBs). This work provides new perspectives on the structural evolution of NaCrO, and the results are expected to contribute to the development of SIBs and PIBs.
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http://dx.doi.org/10.1021/acsami.1c04997DOI Listing
May 2021

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-grade Glioma.

Biosci Rep 2021 May 10. Epub 2021 May 10.

Guangdong Provincial People's Hospital, Guangzhou, China.

Synapse and synapse associated proteins (SAPs) play critical roles in various neurodegeneration diseases and brain tumors. However, in lower-grade gliomas (LGG), SAPs have not been explored systematically. Herein, we are going to explore SAPs expression profile and its clinicopathological significance in LGG which can offer new insights to glioma therapy. In this study, we integrate a list of SAPs that covered 231 proteins with synaptogenesis activity and post synapse formation. The LGG RNA-seq data were downloaded from GEO, TCGA and CGGA database. The prognosis associated SAPs in key modules of PPI (protein-protein interaction networks) was regarded as hub SAPs. Western blot, quantitative reverse transcription PCR (qRT-PCR) and immunochemistry results from HPA database were used to verify the expression of hub SAPs. There were 68 upregulated SAPs and 44 downregulated SAPs in LGG tissue compared with normal brain tissue. Data from function enrichment analysis revealed functions of differentially expressed SAPs in synapse organization and glutamatergic receptor pathway in LGGs. Survival analysis revealed that four SAPs, GRIK2, GABRD, GRID2 and ARC were correlate with the prognosis of LGG patients. Interestingly, we found that GABRD were upregulated in LGG patients with seizures, indicating that SAPs may link to the pathogenesis of seizures in glioma patients. The four-SAPs signature was revealed as an independent prognostic factor in gliomas. Our study presented a novel strategy to assess the prognostic risks of LGGs, based on the expression of SAPs.
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http://dx.doi.org/10.1042/BSR20210391DOI Listing
May 2021

Asymmetric Reduction of Aromatic α-Dehydroamino Acid Esters with Water as Hydrogen Source.

J Org Chem 2021 May 9. Epub 2021 May 9.

Key Laboratory of Chemistry in Ethnic Medicinal Resources (Yunnan Minzu University), State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650504, Yunnan, China.

The asymmetric reduction of aromatic α-dehydroamino acid esters with water as the hydrogen source was developed by a Rh/Cu co-catalytic system. The reaction tolerates various functional groups, providing a valuable synthetic tool to access chiral α-amino acid esters readily. Moreover, the present methodology also was applied in the cost-effective and easy to handle preparation of chiral deuterated α-amino esters by using DO.
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http://dx.doi.org/10.1021/acs.joc.1c00426DOI Listing
May 2021

Association of serum uric acid with mortality and cardiovascular outcomes in patients with hypertension: a meta-analysis.

J Thromb Thrombolysis 2021 May 9. Epub 2021 May 9.

Department of Cardiology, The Third Medical Center of PLA General Hospital, No. 69 Yongding Road, Haidian District, Beijing, 100039, China.

Studies on the association of uric acid with mortality and cardiovascular outcomes in patients with hypertension have produced contradictory results. The objective of this meta-analysis was to evaluate the prognostic utility of uric acid in hypertensive patients. We comprehensively searched PubMed and Embase databases (until July 31, 2020) to identify eligible studies that reported the association of uric acid with mortality and cardiovascular outcomes in hypertensive patients. Outcome measures included cardiovascular or all-cause mortality, coronary artery disease (CAD), stroke, and major adverse cardiovascular events (MACEs). Eleven eligible studies of 49,800 hypertensive patients were identified. For patients with the highest than those with reference lower group of uric acid level, the pooled hazard ratio (HR) was 1.51 (95% confidence interval [CI] 1.12-2.02) for all-cause mortality, 1.68 (95% CI 1.28-2.20) for cardiovascular mortality, 1.31 (95% CI 1.10-1.55) for CAD, and 1.48 (95% CI 1.28-1.70) for MACEs. However, there was no significant association between elevated uric acid level and incident stroke in hypertensive patients. This meta-analysis indicates that elevated uric acid level is significantly associated with an increased risk of cardiovascular or all-cause mortality, CAD and MACEs in hypertensive patients. Hypertensive patients with highest uric acid level exhibited a tendency for reduced risk of stroke, but the difference is not significant.
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http://dx.doi.org/10.1007/s11239-021-02453-zDOI Listing
May 2021

COVID-19 Vaccine Hesitancy and Its Determinants Among Adults with a History of Tobacco or Marijuana Use.

J Community Health 2021 May 6. Epub 2021 May 6.

School of Public Health, University of Memphis, 201 Robison Hall, Memphis, TN, 38152, USA.

Decreasing COVID-19 vaccine hesitancy is an urgent challenge in the US. This study aimed to examine COVID-19 vaccine hesitancy among U.S. adults with a history of using tobacco products or marijuana. Data were collected online in 2020-2021 including respondents' willingness to accept a COVID-19 vaccine, factors that may promote vaccine acceptance, and prior history of influenza vaccination. Logistic regression models were conducted to estimate the associations between vaccine hesitancy and demographic characteristics, substance use, the impact of the COVID-19 pandemic, and prior influenza vaccination. Among 387 respondents, 49.1% were willing to receive a COVID-19 vaccine, 26.0% were unwilling, and 24.9% were "not sure." Blacks, suburban or rural residents, those who lived by themselves or with a family with five members or above, those who were not stressed because of the COVID-19 pandemic were more likely to say "no" to a COVID-19 vaccine. Respondents who never, only once, or during some years received an influenza vaccine were 7.0, 6.2, and 5.2 times more likely to say "no" to a COVID-19 vaccine than respondents who received an influenza vaccination every year or almost every year. The use of cigarettes, e-cigarettes, and marijuana, as well as heavy drinking of alcohol, were not associated with COVID-19 hesitancy. The associations between demographic factors and vaccine hesitancy were roughly similar for COVID-19 and influenza vaccines. Although various demographic groups have different levels of vaccine hesitancy and also have different factors to increase their acceptance, addressing misinformation related to COVID-19 vaccines, particularly concern about the safety of the vaccines is crucial in general.
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http://dx.doi.org/10.1007/s10900-021-00993-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101333PMC
May 2021

Development of Prognostic Evaluation Model to Predict the Overall Survival and Early Recurrence of Hepatocellular Carcinoma.

J Hepatocell Carcinoma 2021 29;8:301-312. Epub 2021 Apr 29.

The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian, 350025, People's Republic of China.

Background: The aberrant expressions of lncRNAs have been frequently demonstrated to be closely associated with the prognosis of patients in many cancer types including hepatocellular carcinoma (HCC). Integration of these lncRNAs might provide accurate evaluation of HCC. Therefore, this study aims to develop a novel prognostic evaluation model based on the expression of lncRNAs to predict the survival of HCC patients, postoperatively.

Patients And Methods: RNA sequencing (RNA-seq) analysis was performed for 61 HCC patients (training cohort) to screen prognosis-associated lncRNAs with univariate Cox regression and Log rank test analyses. Multivariate Cox regression analysis was then applied to establish the final model, which was further verified in a validation cohort (n=191). Moreover, performance of the mode was assessed with time-dependent receiver operating characteristic curve (tdROC), Harrell's c-index, and Gönen & Heller's K.

Results: After a serial statistical computation, a novel risk scoring model consisting of four lncRNAs and TNM staging was established, which could successfully divide the HCC patients into low-risk and high-risk groups with significantly different OS and RFS in both training and validation cohorts. tdROC analysis showed that this model achieved a high performance in predicting OS and 2-year RFS in both cohorts. Gene Set Enrichment Analysis revealed that HCC tumor tissues with high-risk score have stronger capacities in immune escape and resistance to treatment.

Conclusion: We successfully established a novel prognostic evaluation model, which exhibited reliable capacity in predicting the OS and early recurrence of HCC patients with relatively higher accuracy.
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http://dx.doi.org/10.2147/JHC.S303330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092946PMC
April 2021

An equity evaluation in stroke inpatients in regard to medical costs in China: a nationwide study.

BMC Health Serv Res 2021 May 5;21(1):425. Epub 2021 May 5.

School of Management, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, 100029, People's Republic of China.

Background: Stroke has always been a severe disease and imposed heavy financial burden on the health system. Equity in patients in regard to healthcare utilization and medical costs are recognized as a significant factor influencing medical quality and health system responsiveness. The aim of this study is to understand the equity in stroke patients concerning medical costs and healthcare utilization, as well as identify potential factors contributing to geographic variation in stroke patients' healthcare utilization and costs.

Methods: Covering 31 provinces in mainland China, our main data were a 5% random sample of stroke claims from Urban Employees Basic Medical Insurance (UEBMI) and Urban Residents Basic Medical Insurance (URBMI) from 2013 to 2016. The Theil index was employed to evaluate the equity in stroke patients in regard to healthcare utilization and medical costs, and the random-effect panel model was used to explore the impact of province-level factors (health resource factors, enabling factors, and economic factors) on medical costs and health care utilization.

Results: Stroke patients' healthcare utilization and medical costs showed significant differences both within and between regions. The UEBMI scheme had an overall lower Theil index value than the URBMI scheme. The intra-region Theil index value was higher than the inter-region Theil index, with the Theil index highest within eastern China, China's richest and most developed region. Health resource factors and enabling factors (represented by reimbursement rate and education attainment years) were identified significantly associated with medical costs (P < 0.05), but have no impact on average length of stay.

Conclusions: China's fragmented urban health insurance schemes require further reform to ensure better equity in healthcare utilization and medical costs for stroke patients. Improving education attainment, offering equal access to healthcare, allocating health resources reasonably and balancing health services prices in different regions also count.
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http://dx.doi.org/10.1186/s12913-021-06436-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097888PMC
May 2021

Repeated Neonatal Isoflurane Exposure is Associated with Higher Susceptibility to Chronic Variable Stress-induced Behavioural and Neuro-inflammatory Alterations.

Neuroscience 2021 May 2. Epub 2021 May 2.

Department of Anaesthesiology and Operating Theatre Services, Xiangya Hospital of Central South University, Changsha City, Hunan Province, PR China; Teaching and Research Section of Anesthesia and Critical Care Medicine, Xiangya Hospital of Central South University, Changsha City, Hunan Province, PR China. Electronic address:

Numerous studies have reported that prolonged or multiple exposures to anaesthetics in early life lead to detrimental effects on brain function, most having focused on neurocognitive function, and relatively few on long term neuropsychiatric performance. The present study investigated the impact of repeated neonatal isoflurane exposure on chronic variable stress (CVS)-induced psychiatric and behavioural outcomes together with CVS-related neuronal activity and neuro-inflammatory reactivity in relevant brain circuits. In the present study, C57BL/6J mice received either three exposures to isoflurane at postnatal days 7, 8, and 9 or a control exposure. From postnatal day 45, mice were exposed to a mild, 3-week, CVS paradigm or none and the CVS-related neuropsychiatric performance was evaluated using a series of behavioural tests. The neuronal activity in relevant brain regions was measured by ΔFosB immunopositivity and CVS-related neuroinflammation was assessed by analysing levels of pro-inflammatory cytokines IL-1α, IL-1β, IL-6, and TNF-α. In mice experiencing serial neonatal isoflurane exposure, we detected a significant enhancement in anxiety levels following CVS procedures, together with enhanced neuronal activity, and exacerbated neuroinflammation in the basolateral amygdaloid nuclei (BLA) and hippocampal dentate gyrus (DG) regions. No such change was found in control mice. These results indicate an association between early multiple isoflurane exposures in infant mice and susceptibility to a CVS-evoked anxious phenotype accompanied by enhanced neuronal activity in BLA and DG regions and high inflammatory reactivity in response to CVS.
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http://dx.doi.org/10.1016/j.neuroscience.2021.04.024DOI Listing
May 2021

Cost-effectiveness of postmastectomy hypofractionated radiation therapy vs conventional fractionated radiation therapy for high-risk breast cancer.

Breast 2021 Apr 19;58:72-79. Epub 2021 Apr 19.

State Key Laboratory of Molecular Oncology and Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China. Electronic address:

Background: The phase 3 NCT00793962 trial demonstrated that postmastectomy hypofractionated radiation therapy (HFRT) was noninferior to conventional fractionated radiation therapy (CFRT) in patients with high-risk breast cancer. This study assessed the cost-effectiveness of postmastectomy HFRT vs CFRT based on the NCT00793962 trial.

Methods: A Markov model was adopted to synthesize the medical costs and health benefits of patients with high-risk breast cancer based on data from the NCT00793962 trial. Main outcomes were discounted lifetime costs, quality-adjusted life-years (QALYs), and incremental cost-effectiveness ratio (ICER). We employed a time-dependent horizon from Chinese, French and USA payer perspectives. Model robustness was evaluated with one-way and probabilistic sensitivity analyses.

Results: Patients receiving CFRT versus HFRT gained an incremental 0.0163 QALYs, 0.0118 QALYs and 0.0028 QALYs; meanwhile an incremental cost of $2351.92, $4978.34 and $8812.70 from Chinese, French and USA payer perspectives, respectively. Thus CFRT versus HFRT yielded an ICER of $144,281.47, $420,636.10 and $3,187,955.76 per QALY from Chinese, French and USA payer perspectives, respectively. HFRT could maintain a trend of >50% probabilities of cost-effectiveness below a willingness-to-pay (WTP) of $178,882.00 in China, while HFRT was dominant relative to CFRT, regardless of the WTP values in France and the USA. Sensitivity analyses indicated that the ICERs were most sensitive to the parameters of overall survival after radiotherapy.

Conclusions: Postmastectomy HFRT could be used as a cost-effective substitute for CFRT in patients with high-risk breast cancer and should be considered in appropriately selected patients.
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http://dx.doi.org/10.1016/j.breast.2021.04.002DOI Listing
April 2021

Histone deacetylase 3 suppresses the expression of SHP-1 via deacetylation of DNMT1 to promote heart failure.

Life Sci 2021 Apr 28:119552. Epub 2021 Apr 28.

School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.

Aims: Heart failure (HF) is a progressive disease with recurrent hospitalizations and high mortality. However, the mechanisms underlying HF remain unclear. The present study aimed to explore the regulatory mechanism of histone deacetylase 3 (HDAC3) and DNA methyltransferase 1 (DNMT1)/Src homology domain 2-containing tyrosine phosphatase-1 (SHP-1) axis in HF.

Methods: The HF rat models and hypertrophy cell models were established. The characteristic parameters of the heart were detected by echocardiography. A multichannel physiological signal acquisition system was used to detect the hemodynamic parameters. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of HDAC3, DNMT1, and SHP-1 mRNAs, while Western blot was applied to analyze the expression of proteins. Masson staining was used to analyze the degree of collagen fiber infiltration. TdT-mediated DUTP nick end labeling (TUNEL) staining was performed to analyze the apoptosis of myocardial tissue cells. Co-immunoprecipitation (co-IP) was conducted to study the interaction between HDAC3 and DNMT1. Flow cytometry was used to analyze the apoptosis.

Key Findings: HDAC3 and DNMT1 were highly expressed in HF rat and hypertrophy cell models. HDAC3 modified DNMT1 through deacetylation to inhibit ubiquitination-mediated degradation, which promoted the expression of DNMT1. DNMT1 inhibited SHP-1 expression via methylation in the promoter region. In summary, HDAC3 modified DNMT1 by deacetylation to suppress SHP-1 expression, which in turn led to the development of cardiomyocyte hypertrophy-induced HF.

Significance: This study provided potential therapeutic targets for HF treatment.
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http://dx.doi.org/10.1016/j.lfs.2021.119552DOI Listing
April 2021

Tumor Biological Feature and Its Association with Positive Surgical Margins and Apical Margins after Radical Prostatectomy in Non-Metastasis Prostate Cancer.

Curr Oncol 2021 Apr 13;28(2):1528-1536. Epub 2021 Apr 13.

Key Laboratory of Carcinogenesis and Translational Research (Mninistry of Education), Urological Department, Peking University Cancer Hospital & Institute, Beijing 100142, China.

Purpose: We assessed clinical and tumor biological features and evaluated their association with positive surgical margins (PSMs) and positive apical margins (PAMs) variability after radical prostatectomy (RP) in men with non-metastasis prostate cancer (nmPCa) in our institute.

Patients And Methods: During the period from January 2013 to December 2017, clinical and pathological data were collected in 200 patients with nmPCa undergoing RP in the Urological department of Peking University Cancer Hospital & Institute. Surgical and apical margins were stated negative and positive, separately. A dichotomous logistic regression model was used to assess clinical and tumor biological features including age, total prostate volume (TPV), biopsy positive cores (BPC), D'Amico risk grade, tumor clinical stage, International Society of Urologic Pathology (ISUP) grade, tPSA, f/t and pelvic lymph nodes (PLN) invasion, and their association with PSMs and PAMs was evaluated.

Results: Overall, men with nmPCa in this study had a high ISUP grade (58.5% grade 3-5), high risk grade (89.4%) and high clinical T stage (56% cT3-4). PSMs were detected in 106 patients; the rate of PSMs was 53%. Among patients with PSMs, 83% were PAMs; the overall rate of PAMs was 44%. Among patients with PSMs, high risk (OR, 1.439; = 0.023), cT3a (OR, 1.737; = 0.045), cT3b (OR, 5.286; < 0.001), cT4 (OR, 6.12; < 0.001), ISUP Grade 4 (OR, 2; = 0.034) and Grade 5 (OR, 6.167; < 0.001) and PLN invasion (OR, 6; = 0.019) were strongly associated with PSMs using a dichotomous logistic regression univariable model, and high risk (OR, 6; = 0.019), cT3a (OR, 5.116; = 0.048), cT3b (OR, 9.194; = 0.008), cT4 (OR, 4.58; = 0.01), ISUP Grade 4 (OR, 7.04; = 0.035), Grade 5 (OR, 16.514; = 0.002) and PLN invasion (OR, 5.516; = 0.03) were independently associated with PSMs by using multivariable analysis. Among patients with PAMs, cT3b (OR, 2.667; = 0.004), cT4 (OR, 3; = 0.034) and proportion of BPC (OR, 4.594; = 0.027) were strongly associated with PAMs by using a dichotomous logistic regression univariable model, and cT3b (OR, 3.899; = 0.02), cT4 (OR, 2.8; = 0.041) and proportion of BPC (OR, 5.247; = 0.04) were independently associated with PSMs by using multivariable analysis.

Conclusions: Patients with nmPCa in our institute had high risk, high ISUP grade and high clinical stage. Tumor biological factors were strongly associated with PSMs and PAMs, and PLN invasion was independently associated with PSMs. The risk factors influenced the status of surgical margins, and apical margins were different.
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http://dx.doi.org/10.3390/curroncol28020144DOI Listing
April 2021

SiC Nanowire-SiN Nanobelt Interlocking Interfacial Enhancement of Carbon Fiber Composites with Boosting Mechanical and Frictional Properties.

ACS Appl Mater Interfaces 2021 May 26;13(17):20746-20753. Epub 2021 Apr 26.

Faculty of Engineering, The University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.

Carbon fiber composites composed of carbon fiber and pyrolytic carbon (PyC) matrix have great potential application in the brakes of aircrafts, where the combination of high mechanical strength and excellent frictional properties are required. In this work, two-component silicon-based interlocking enhancements were designed and constructed into carbon fiber composites for boosting the mechanical and frictional properties. Specially, silicon carbide nanowires (SiCnws) and silicon nitride nanobelts (SiNnbs) could form interlocking architectures, where SiCnws are rooted firmly on the carbon fiber surface in the radial direction and SiNnbs integrate the PyC matrix with carbon fibers together via a networked shape. SiCnws-SiNnbs not only refine the PyC matrix but also promote the bonding of the fiber/matrix interface and the cohesion strength of the PyC matrix, thus enhancing the mechanical and frictional properties. Benefiting from the SiCnws-SiNnbs synergistic effect and interlocking enhancement mechanism, the interlaminar shear strength and compressive strength of carbon fiber composites increased by 88.41% and 73.40%, respectively. In addition, the friction coefficient and wear rate of carbon fiber composites decreased by 39.50% and 69.88%, respectively. This work could open up an interlocking enhancement strategy for efficiently fabricating carbon fiber composites and promoting mechanical and frictional properties that could be used in the brakes of aircrafts.
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http://dx.doi.org/10.1021/acsami.1c04682DOI Listing
May 2021

High-efficient L-lactic acid production from inedible starchy biomass by one-step open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908.

Bioprocess Biosyst Eng 2021 Apr 22. Epub 2021 Apr 22.

School of Bioengineering, Dalian University of Technology, No.2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province, 116024, People's Republic of China.

The purpose of this study was to establish a simplified operational process for lactic acid (LA) production from inedible starchy biomass by open fermentation using thermotolerant Lactobacillus rhamnosus DUT1908. One step simultaneous liquefaction, saccharification and fermentation (SLSF) was proposed to produce LA using aging paddy rice with hull (APRH) as feedstock. First, a robust microbial strain was obtained by adaptive laboratory evolution under high temperature stress. As a result, L. rhamnosus DUT1908 showed high thermotolerance up to 50 °C and high efficiency of substrate utilization. Then, the performance of this thermotolerant L-lactic acid producing strain was demonstrated. Finally, various fermentation strategies were compared for LA production from APRH, including simultaneous saccharification and fermentation (SSF) and SLSF. In one-step open SLSF process, 107.8 g/L lactic acid was obtained with a productivity of 3.4 g/(L.h) and a yield to theoretical glucose of 0.89 g/g. This is the highest yield and productivity of lactic acid reported on starchy residues, and provides an efficient route for the development of high value-added products.
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http://dx.doi.org/10.1007/s00449-021-02573-zDOI Listing
April 2021

Magnetic-Field Directed Vapor-Phase Assembly of Low Fractal Dimension Metal Nanostructures: Experiment and Theory.

J Phys Chem Lett 2021 Apr 22;12(16):4085-4091. Epub 2021 Apr 22.

Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, United States.

While gas-phase synthesis techniques offer a scalable approach to production of metal nanoparticles, directed assembly is challenging due to fast particle diffusion rates that lead to random Brownian aggregation. This work explores an electromagnetic-levitation technique to generate metal nanoparticle aggregates with fractal dimension () below that of diffusion limited assembly. We demonstrate that in addition to levitation and induction heating, the external magnetic field is sufficient to compete with random Brownian forces, which enables the formation of altered fractals. Ferromagnetic metals (Fe, Ni) form chain-like aggregates, while paramagnetic Cu forms compact nanoparticle aggregates with higher values. We have also employed a Monte Carlo simulation to evaluate the necessary field strength to form linear chains in the gas phase.
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http://dx.doi.org/10.1021/acs.jpclett.0c03463DOI Listing
April 2021

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury.

Mol Med Rep 2021 06 21;23(6). Epub 2021 Apr 21.

Department of Pathophysiology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

Our previous study reported that microRNA (miR)‑30a‑5p upregulation under hypoxia postconditioning (HPostC) exert a protective effect on aged H9C2 cells against hypoxia/reoxygenation injury via DNA methyltransferase 3B‑induced DNA hypomethylation at the miR‑30a‑5p gene promoter. This suggests that miR‑30a‑5p may be a potential preventative and therapeutic target for ischemic heart disease in aged myocardium. The present study aimed to investigate the underlying mechanisms of miR‑30a‑5p transcription in aged myocardium in ischemic heart disease. Cardiomyocytes were treated with 8 mg/ml D‑galactose for 9 days, and then exposed to hypoxic conditions. Cell viability was determined using a cell viability assay. Expression levels of histone deacetylase 2 (HDAC2), LC3B‑II/I, beclin‑1 and p62 were detected via reverse transcription‑quantitative PCR and western blotting. Chromatin immunoprecipitation‑PCR and luciferase reporter assays were performed to evaluate the effect of c‑Myc binding and activity on the miR‑30a‑5p promoter in senescent cardiomyocytes following HPostC. It was found that HPostC enhanced the acetylation levels of H3K14 at the miR‑30a‑5p gene promoter in senescent cardiomyocytes, which attributed to the decreased expression of HDAC2. In addition, c‑Myc could positively regulate miR‑30a‑5p transcription to inhibit senescent cardiomyocyte autophagy. Mechanically, it was observed that increased H3K14 acetylation level exposed to romidepsin facilitated c‑Myc binding to the miR‑30a‑5p gene promoter region, which led to the increased transcription of miR‑30a‑5p. Taken together, these results demonstrated that HDAC2‑mediated H3K14 hyperacetylation promoted c‑Myc binding to the miR‑30a‑5p gene promoter, which contributed to HPostC senescent cardioprotection.
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http://dx.doi.org/10.3892/mmr.2021.12107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097758PMC
June 2021

The pan-cancer noninvasive screening and early detection by epigenetic techniques.

Epigenomics 2021 May 20;13(9):649-652. Epub 2021 Apr 20.

Department of Radiotherapy, The Eighth Medical Center of The Chinese PLA General Hospital, Beijing 100091, PR China.

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http://dx.doi.org/10.2217/epi-2021-0063DOI Listing
May 2021

Serological investigation of asymptomatic cases of SARS-CoV-2 infection reveals weak and declining antibody responses.

Emerg Microbes Infect 2021 Apr 19:1-28. Epub 2021 Apr 19.

CAS Key Laboratory of Special Pathogens & State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.

Without an effective vaccine against SARS-CoV-2, the build-up of herd immunity through natural infection has been suggested as a means to control COVID-19. Although population immunity is typically estimated by the serological investigation of recovered patients, humoral immunity in asymptomatic subjects has not been well studied, although they represent a large proportion of all SARS-CoV-2 infection cases. In this study, we conducted a serosurvey of asymptomatic infections among food workers and performed serological and cellular response analyses of asymptomatic subjects in Wuhan, the original epicenter of the COVID-19 outbreak. Our data showed that up to 5.91% of the food workers carried SARS-CoV-2 IgG antibodies asymptomatically; however, in 90.4% of them, the antibody level declined over a 2-week period. IgM and IgG antibodies, including neutralizing antibodies, were significantly lower in asymptomatic subjects than in recovered symptomatic patients with similar disease courses. Furthermore, the asymptomatic subjects showed lymphopenia and a prominent decrease in the B-cell population, as well as a low frequency of antibody-secreting cells and a low cytokine response. These factors probably contributed to the low and unsustained antibody levels in asymptomatic subjects. Our results show that asymptomatic subjects are likely to be vulnerable to SARS-CoV-2 reinfection, and neither the proportion of population immunity nor the breadth of immune responses is sufficient for herd immunity.
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http://dx.doi.org/10.1080/22221751.2021.1919032DOI Listing
April 2021

Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection.

Nanomicro Lett 2021 Dec 13;13:109. Epub 2021 Apr 13.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200032 People's Republic of China.

The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.

Supplementary Information: The online version contains supplementary material available at 10.1007/s40820-021-00620-8.
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http://dx.doi.org/10.1007/s40820-021-00620-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042470PMC
December 2021

A Novel Methodology for Semi-automatic Measurement of Arterial Stiffness by Doppler Ultrasound: Clinical Feasibility and Reproducibility.

Ultrasound Med Biol 2021 Apr 12. Epub 2021 Apr 12.

Department of Ultrasound Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

The conventional manual approach to measurement of aortic pulse wave velocity (PWV) by Doppler ultrasonography is time consuming and operator dependent. Here we report a new semi-automated methodology for more efficient and objective measurement of aortic PWV and results of tests of its clinical feasibility and reproducibility. Carotid-femoral pulse wave velocity (cfPWV) was measured in 50 patients with suspected coronary artery disease (aged 59.2 ± 10.0 y, 36 males) by three independent observers, including two experienced sonographers and one cardiologist without ultrasonographic experience. The cfPWV measured by the semi-automatic method (cfPWV) was compared with reference values obtained by averaging measurements by two experienced sonographers using the conventional standard manual method (cfPWV). Measurements of cfPWV were feasible in all 50 patients and exhibited excellent agreement with averaged cfPWV from the two experienced sonographers, with an intraclass correlation coefficient (ICC) of 0.915 (95% confidence interval: 0.876-0.942). The inexperienced observer-measured cfPWV did not differ from the cfPWV measured by the two experienced sonographers (8.04 ± 1.29 vs. 8.14 ± 1.32 m/s, p > 0.05), with a high consistency by ICC of 0.877 (0.793-0.928). Bland-Altman plots further illustrated the good agreement between the two methods and good intra- and inter-observer reproducibility. Time consumption for cfPWV measurement using the new method was significantly less than that for the manual method (122 ± 35 s vs. 455 ± 105 s, p < 0.0001), saving about 73% of the time. This new semi-automatic methodology for aortic PWV measurement not only has an accuracy similar to that of the conventional standard manual method but is also highly feasible and time saving. It may provide a reliable, simple and reproducible approach to arterial stiffness evaluation in clinical settings.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.03.004DOI Listing
April 2021

Anesthetic management for resection of a giant emphysematous bulla: a case report.

J Int Med Res 2021 Apr;49(4):3000605211001989

Department of Anesthesiology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou 317000, P.R. China.

Anesthetic management for patients with a giant emphysematous bulla (GEB) is challenging. This case report describes a patient who developed 95% pulmonary compression by a GEB. A 14-Ga indwelling catheter was placed in the GEB before surgery to allow for slow re-expansion of the collapsed lung tissue. This prevented rupture of the GEB during anesthesia. Additionally, positive-pressure ventilation was performed to reduce the risk of re-expansion pulmonary edema. This respiratory management strategy may be beneficial for patients with a GEB who develop pulmonary dysfunction during thoracic surgery.
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http://dx.doi.org/10.1177/03000605211001989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059042PMC
April 2021

The Exploration of Novel Pharmacophore Characteristics and Multidirectional Elucidation of Structure-Activity Relationship and Mechanism of Sesquiterpene Pyridine Alkaloids from Based on Computational Approaches.

Evid Based Complement Alternat Med 2021 24;2021:6676470. Epub 2021 Mar 24.

Chongqing Academy of Chinese Materia Medica, No. 34 Nanshan Road, Nan'an District, Chongqing 400065, China.

Sesquiterpene pyridine alkaloids are a large group of highly oxygenated sesquiterpenoids, which are characterized by a macrocyclic dilactone skeleton containing 2-(carboxyalkyl) nicotinic acid and dihydro--agarofuran sesquiterpenoid, and are believed to be the active and less toxic components of . In this study, 55 sesquiterpene pyridine alkaloids from were subjected to identification of pharmacophore characteristics and potential targets analysis. Our results revealed that the greatest structural difference of these compounds was in the pyridine ring and the pharmacophore model-5 (Pm-05) was the best model that consisted of three features including hydrogen bond acceptor (HBA), hydrogen bond donor (HBD), and hydrophobic (HY), especially hydrophobic group located in the pyridine ring. It was proposed that 2-(carboxyalkyl) nicotinic acid part possessing a pyridine ring system was not only a pharmacologically active center but also a core of structural diversity of alkaloids from . Furthermore, sesquiterpene pyridine alkaloids from were predicted to target multiple proteins and pathways and possibly played essential roles in the cure of Alzheimer's disease, breast cancer, Chagas disease, and nonalcoholic fatty liver disease (NAFLD). They also had other pharmacological effects, depending on the binding interactions between pyridine rings of these compounds and active cavities of the target genes platelet-activating factor receptor (PTAFR), cannabinoid receptor 1 (CNR1), cannabinoid receptor 1 (CNR2), squalene synthase (FDFT1), and heat shock protein HSP 90-alpha (HSP90AA1). Taken together, the results of this present study indicated that sesquiterpene pyridine alkaloids from are promising candidates that exhibit potential for development as medicine sources and need to be promoted.
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http://dx.doi.org/10.1155/2021/6676470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012133PMC
March 2021

Correlation between early brain natriuretic peptide level and mortality in cancer patients with septic shock.

Ann Palliat Med 2021 Apr 23;10(4):4214-4219. Epub 2021 Mar 23.

Department of Hepato-Pancreato-Biliary Surgery, Peking University Cancer Hospital & Institute, Beijing, China.

Background: This study aimed to examine the correlation between early brain natriuretic peptide (BNP) levels and mortality in cancer patients with septic shock.

Methods: A retrospective analysis of 159 cancer patients with septic shock admitted to the intensive care unit (ICU) from Dec. 2012 to Dec. 2019 was performed. BNP levels and other variables, including blood lactate (Lac), procalcitonin (PCT), white blood cell (WBC) counts, acute physiology and chronic health status system II scores (APACHE-II scores) were collected within 24 hours after ICU admission. According to 28-day mortality, patients were divided into a death group (60 cases) and a survival group (99 cases). All variables were compared by univariate analysis, and then a multiple logistic regression analysis was performed on the variables that showed significant differences. Receiver operating characteristic curve (ROC curve) analysis was used to evaluate the predictive value of BNP on mortality in cancer patients with septic shock.

Results: BNP, APACHE-II score, Lac, and PCT in the death group were significantly higher than those in the survival group (P<0.05). Multiple logistic regression analysis of these four variables indicated that BNP, APACHE-II score and Lac were independent risk predictors of mortality in these patients (P<0.05). The BNP level at 899.6 pg/mL predicted mortality with a sensitivity of 76.7% and a specificity of 84.7%. The area under the ROC curve was 0.86±0.03 (P<0.05) for BNP, which was significantly larger than that of the APACHE-II score (P<0.05) and Lac (P<0.05).

Conclusions: BNP was an independent risk factor for mortality in cancer patients with septic shock, and had a higher predictive value than the APACHE-II score and Lac.
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http://dx.doi.org/10.21037/apm-20-2212DOI Listing
April 2021

Comparative life cycle assessment of emergency disposal scenarios for medical waste during the COVID-19 pandemic in China.

Waste Manag 2021 Mar 26;126:388-399. Epub 2021 Mar 26.

College of Materials and Engineering, Tianjin Chengjian University, Tianjin 300384, China.

The COVID-19 pandemic attracts concerns globally and leads to an exponential increase in medical waste generation, and disposal of medical waste is an urgent need for preventing the epidemic spread. Emergency disposal scenarios of medical waste generated during the COVID-19 pandemic require a systematic assessment to quantify their potential environmental impacts. The environmental impacts and key factors of three movable disposal scenarios (i.e. incineration disposal vehicle, movable steam and microwave sterilization equipment both followed by co-incineration with municipal solid waste) were quantified via life cycle assessment approach. Furthermore, the environmental impacts of three movable disposal and two co-incineration scenarios were compared via life cycle assessment by expanding system boundaries. The results show that co-incineration with municipal solid waste has the lowest environmental impacts due to environmental benefits produced by power generation, while co-incineration with hazardous waste is the highest due to the high energy consumption. Energy consumption (i.e. kerosene, electricity and diesel) are the key factors for three movable disposal scenarios. For movable steam and microwave sterilization equipment followed by co-incineration with municipal solid waste, power generation from incinerating disinfected medical waste has significant beneficial environmental impacts due to avoided impacts of electricity consumption. The recommendations for improvement of the emergency disposal and management of medical waste during the COVID-19 pandemic globally and other serious epidemic in the future are provided.
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http://dx.doi.org/10.1016/j.wasman.2021.03.034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997725PMC
March 2021

Effective remediation of petrochemical originated pollutants using engineered materials with multifunctional entities.

Chemosphere 2021 Mar 29;278:130405. Epub 2021 Mar 29.

Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, 64849, Mexico. Electronic address:

The highly robust, effective, and sustainable remediation of hydrocarbon-contaminated wastewater matrices, which is mainly generated from petroleum and related petrochemical industries, is of supreme interest. Owing to the notable presence of suspended solids, oil, and grease, organic matter, highly toxic elements, high salts, and recalcitrant chemicals, crude oil emulsions, and hydrocarbon-contaminated wastewater are considered a potential threat to the environments, animals, plants, and humans. To effectively tackle this challenging issue, magnetic hybrid materials assembled at nano- and micro-scale with unique structural, chemical, and functional entities are considered robust candidates for demulsification purposes. The current research era on magnetic materials has superwettability, leading to an effective system of superwettability, which is vibrant and promising. The wettability of magnetic and magnetic hybrid materials explaining the theme of superhydrophobicity and superhydrophilicity under the liquid. Herein, we reviewed the applications of magnetic nanoparticles (MNPs) as effective demulsifiers. The demulsifier wettability, dose, pH, salinity, and surface morphology of compelling, magnetic nanoparticles are the main hidden factors in effective demulsifiers. There is a comprehensive discussion on the reuse and recyclability of MNPs after oil, water separation. Furthermore, the main challenges, coupled with the magnetic nanoparticles in the effective separation of emulsions, are intensified in detail. This review will compare the current literature and the utilization of MNPs for the demulsification of oil and water emulsions. This is envisioned that the MNPs would be critical in the petroleum and petrochemical industry to effectively eliminate water from a crude oil emulsion.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130405DOI Listing
March 2021

Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by Excretory Secretory Products of Might Be Related to Serine Protease.

Front Microbiol 2021 17;12:634185. Epub 2021 Mar 17.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, China.

The physical barrier is composed of epithelial cells which are joined together through intercellular connections. It serves to prevent pathogenic microorganisms from departing the intestinal lumen to invade the host. The excretory secretory (ES) products of are critical for invasion. However, whether ES products of can act on the intestinal barrier is still unknown. In this study, the role of ES products of muscle larvae (-ML-ES) in host invasion was studied by establishing an cell monolayers model. Barrier integrity analysis by a transmembrane resistance test and a paracellular permeability assay revealed that the -ML-ES was able to destroy barrier function. It occurred a reduction in the expression of tight junction (TJ) proteins, which was induced by serine protease. Furthermore, Western bolt analysis indicated that -ML-ES reduced the expression of TJ proteins the MAPK signaling pathway. Based on these data, we conclude that serine protease are likely the main factors from -ML-ES that affect host intestinal barrier integrity by reducing the expression of TJs the P38-MAPK signaling pathway. Serine protease in -ML-ES might be a key invasion factor in .
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http://dx.doi.org/10.3389/fmicb.2021.634185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013981PMC
March 2021

The complete chloroplast genome of (Sapotaceae), an endemic and endangered timber species in Hainan Island, China.

Mitochondrial DNA B Resour 2021 Mar 15;6(3):755-757. Epub 2021 Mar 15.

Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, China.

Chun & F.C.How is an endangered and endemic species in Hainan Island, and it was ranked as a VU (Vulnerable) species in China. In this study, we reported its complete chloroplast (cp) genome sequence based on high throughput sequencing data. The complete cp genome was 159,630 bp in length, containing two short inverted repeat (IRs) of 26,093 bp, a large single copy (LSC) region of 88,846 bp and a small single copy (SSC) region of 18,598 bp. Totally, the cp genome contained 131 genes, including 86 protein coding genes (PCG), eight rRNA genes and 37 tRNAs. The GC contents of this genome was 36.8%. A maximum likelihood (ML) phylogenetic analysis indicates that is closely related to .
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http://dx.doi.org/10.1080/23802359.2021.1878954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971245PMC
March 2021