Publications by authors named "Yong Xue"

253 Publications

COVID-19 pandemic decision support system for a population defense strategy and vaccination effectiveness.

Saf Sci 2021 Oct 5;142:105370. Epub 2021 Jun 5.

Department of Environmental Physics and Meteorology, National and Kapodistrian University of Athens, Panepistimioupolis, Bldg PHYS-V, GR-157 84 Athens, Greece.

The year 2020 ended with a significant COVID-19 pandemic, which traumatized almost many countries where the lockdowns were restored, and numerous emotional social protests erupted. According to the World Health Organization, the global epidemiological situation in the first months of 2021 deteriorated. In this paper, the decision-making supporting system (DMSS) is proposed to be an epidemiological prediction tool. COVID-19 trends in several countries and regions, take into account the big data clouds for important geophysical and socio-ecological characteristics and the expected potentials of the medical service, including vaccination and restrictions on population migration both within the country and international traffic. These parameters for numerical simulations are estimated from officially delivered data that allows the verification of theoretical results. The numerical simulations of the transition and the results of COVID-19 are mainly based on the deterministic approach and the algorithm for processing statistical data based on the instability indicator. DMSS has been shown to help predict the effects of COVID-19 depending on the protection strategies against COVID-19 including vaccination. Numerical simulations have shown that DMSS provides results using accompanying information in the appropriate scenario.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ssci.2021.105370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179249PMC
October 2021

Influence of elemental sulfur on cadmium bioavailability, microbial community in paddy soil and Cd accumulation in rice plants.

Sci Rep 2021 Jun 1;11(1):11468. Epub 2021 Jun 1.

ECO-Environment Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.

Cadmium (Cd) is highly toxic to living organisms and the contamination of Cd in paddy soil in China has received much attention. In the present study, by conducting pot experiment, the influence of S fertilizer (S) on rice growth, iron plaque formation, Cd accumulation in rice plants and bacterial community in rice rhizosphere soil was investigated. The biomass of rice plants was significantly increased by S addition (19.5-73.6%). The addition of S increased the formation of iron plaque by 24.3-45.8%, meanwhile the amount of Cd sequestered on iron plaque increased. In soil treated with 5 mg/kg Cd, addition of 0.2 g/kg S decreased the diffusive gradients in thin films (DGT) extractable Cd by 60.0%. The application of S significantly decreased the concentration of Cd in rice grain by 12.1% (0.1 g/kg) and 36.6% (0.2 g/kg) respectively. The addition of S significantly increased the ratio of Acidobacteria, Bacteroidetes in rice rhizosphere soil. Meanwhile, the ratio of Planctomycetes and Chloroflexi decreased. The results indicated that promoting Fe- and S-reducing and residue decomposition bacterial in the rhizosphere by S may be one biological reason for reducing Cd risk in the soil-rice system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-91003-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169911PMC
June 2021

Comparative study on the antituberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan.

Mil Med Res 2021 Jun 2;8(1):34. Epub 2021 Jun 2.

Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Tuberculosis Research Institute, the 8th Medical Center, Chinese PLA General Hospital, Beijing, 100091, China.

Background: The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis effects. The anti- tuberculosis effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH.

Methods: BALB/c mice aged 6-8 weeks were randomly divided into a normal control group, Tuberculosis (TB) model group, JHW treatment group, and NBXH treatment group. After 3 and 13 weeks of treatment, the therapeutic effect in each group was evaluated by comparing lung histopathology, lung and liver colony counts, the number of spots representing effector T cells secreting IFN-γ in an ELISPOT, and the levels of Th1, Th2, and Th17 cytokines, which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing.

Results: After 13 weeks of treatment, the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (P < 0.05). Compared with those in the TB model group, the lung colony counts in the JHW and NBXH groups were significantly decreased (P < 0.05), and the IL-2 and IL-4 levels in the NBXH group were significantly increased (P < 0.05). NBXH partly restored significant changes in gene expression caused by Mycobacterium tuberculosis (M. tuberculosis) infection. According to GO and KEGG analyses, the changes in biological process (BP), cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent, but they were mainly related to the immune response and inflammatory response in the mouse TB model.

Conclusions: NBXH had therapeutic effects similar to those of JHW in improving lung histopathology, reducing lung colony counts, and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by M. tuberculosis infection by regulating immune-related pathways, which clarified the mechanism of action of NBXH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40779-021-00324-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170785PMC
June 2021

Foxtail Millet Improves Blood Glucose Metabolism in Diabetic Rats through PI3K/AKT and NF-κB Signaling Pathways Mediated by Gut Microbiota.

Nutrients 2021 May 27;13(6). Epub 2021 May 27.

Key Laboratory of Plant Protein and Grain Processing, National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Foxtail millet (FM) is receiving ongoing increased attention due to its beneficial health effects, including the hypoglycemic effect. However, the underlying mechanisms of the hypoglycemic effect have been underexplored. In the present study, the hypoglycemic effect of FM supplementation was confirmed again in high-fat diet and streptozotocin-induced diabetic rats with significantly decreased fasting glucose (FG), glycated serum protein, and areas under the glucose tolerance test ( < 0.05). We employed 16S rRNA and liver RNA sequencing technologies to identify the target gut microbes and signaling pathways involved in the hypoglycemic effect of FM supplementation. The results showed that FM supplementation significantly increased the relative abundance of Lactobacillus and Ruminococcus_2, which were significantly negatively correlated with FG and 2-h glucose. FM supplementation significantly reversed the trends of gene expression in diabetic rats. Specifically, FM supplementation inhibited gluconeogenesis, stimulated glycolysis, and restored fatty acid synthesis through activation of the PI3K/AKT signaling pathway. FM also reduced inflammation through inhibition of the NF-κB signaling pathway. Spearman's correlation analysis indicated a complicated set of interdependencies among the gut microbiota, signaling pathways, and metabolic parameters. Collectively, the above results suggest that the hypoglycemic effect of FM was at least partially mediated by the increased relative abundance of Lactobacillus, activation of the PI3K/AKT signaling pathway, and inhibition of the NF-κB signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu13061837DOI Listing
May 2021

Protein structural properties and proteomic analysis of rice during storage at different temperatures.

Food Chem 2021 Nov 9;361:130028. Epub 2021 May 9.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruit and Vegetable Processing, Beijing 100083, China; Key Laboratory of Plant Protein and Grain Processing, Beijing 100083, China. Electronic address:

Rice quality changes during storage, but there have been few studies of how rice proteins changes during aging. The present study characterized the structural properties of protein in stored rice and identified the mechanism of quality deterioration using proteomics. Compared with protein from newly harvested rice, the free sulfhydryl content of protein from stored rice was significantly reduced and the disulfide bond content and surface hydrophobicity was higher. Storage resulted in a loss of α-helix and β-sheet structures and increase in β-turn and random coil structures. High-molecular-weight protein subunits decomposed to produce low-molecular-weight subunits at 30 °C, while protein aggregation occurred at 70 °C. At 30 ℃ 157 differential proteins were found and 70 ℃ 395 such proteins occurred. Redox homeostasis, response to oxidative stress, glutathione metabolism, tricarboxylic acid cycle, glycolysis/gluconeogenesis, starch and sucrose metabolism, and fatty acid biosynthesis and degradation led to the different quality of stored rice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130028DOI Listing
November 2021

Electrochemical ammonia synthesis via nitrate reduction on Fe single atom catalyst.

Nat Commun 2021 May 17;12(1):2870. Epub 2021 May 17.

Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA.

Electrochemically converting nitrate, a widespread water pollutant, back to valuable ammonia is a green and delocalized route for ammonia synthesis, and can be an appealing and supplementary alternative to the Haber-Bosch process. However, as there are other nitrate reduction pathways present, selectively guiding the reaction pathway towards ammonia is currently challenged by the lack of efficient catalysts. Here we report a selective and active nitrate reduction to ammonia on Fe single atom catalyst, with a maximal ammonia Faradaic efficiency of ~ 75% and a yield rate of up to ~ 20,000 μg h mg (0.46 mmol h cm). Our Fe single atom catalyst can effectively prevent the N-N coupling step required for N due to the lack of neighboring metal sites, promoting ammonia product selectivity. Density functional theory calculations reveal the reaction mechanisms and the potential limiting steps for nitrate reduction on atomically dispersed Fe sites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23115-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128876PMC
May 2021

Peptides-Based Vaccine MP3RT Induced Protective Immunity Against Infection in a Humanized Mouse Model.

Front Immunol 2021 26;12:666290. Epub 2021 Apr 26.

Tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute for Tuberculosis Research, 8th Medical Center, Chinese People's Liberation Army General Hospital, Beijing, China.

Background: Tuberculosis (TB) is still a global infectious disease that seriously threatens human beings. The only licensed TB vaccine Bacille Calmette-Guérin (BCG)'s protective efficacy varies significantly among populations and regions. It is very urgent to develop more effective vaccines.

Methods: In this study, eleven candidate proteins of were selected to predict peptides with high-affinity binding capacity for the HLA-DRB1*01:01 molecule. The immunodominant peptides were identified with the enzyme-linked immunospot assay (ELISPOT) and linked in silico to result in a novel polypeptide vaccine in cells. The vaccine's protective efficacy was evaluated in humanized and wild-type C57BL/6 mice. The potential immune protective mechanisms were explored with Enzyme-linked Immunosorbent Assay (ELISA), flow cytometry, and ELISPOT.

Results: Six immunodominant peptides screened from 50 predicted peptides were used to construct a new polypeptide vaccine named MP3RT. After challenge with , the colony-forming units (CFUs), lung lesion area, and the number of inflammatory cells in humanized mice rather than wild-type mice vaccinated with MP3RT were significantly lower than these in mice immunized with PBS. The humanized mice vaccinated with MP3RT revealed significant increases in IFN-γ cytokine production, IFN-γ T lymphocytes, CD3IFN-γ T lymphocytes, and the MP3RT-specific IgG antibody.

Conclusions: Taken together, MP3RT is a promising peptides-based TB vaccine characterized by inducing high levels of IFN-γ and CD3IFN-γ T lymphocytes in humanized mice. These new findings will lay a foundation for the development of peptides-based vaccines against TB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.666290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108698PMC
April 2021

Pressure-Triggered Blue Emission of Zero-Dimensional Organic Bismuth Bromide Perovskite.

Adv Sci (Weinh) 2021 May 15;8(9):2004853. Epub 2021 Feb 15.

Green Catalysis Center and College of Chemistry Zhengzhou University Zhengzhou 450001 P. R. China.

Understanding the structure-property relationships in Zero-dimensional (0D) organic-inorganic metal halide perovskites (s) is essential for their use in optoelectronic applications. Moreover, increasing the emission intensity, particularly for blue emission, is considerably a challenge. Here, intriguing pressure-induced emission () is successfully achieved from an initially nonluminous 0D [(CHNH)BiBr]Br·CHCN ( ) upon compression. The emission intensity increases significantly, even reaching high-efficiency blue luminescence, as the external pressure is increased to 4.9 GPa. Analyses of the high-pressure experiments and first-principle calculations indicate that the observed PIE can be attributed to the enhanced exciton binding energy associated with [BiBr] octahedron distortion under pressure. This study of sheds light on the relationship between the structure and optical properties of s. The results may improve potential applications of such materials in the fields of pressure sensing and trademark security.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202004853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097370PMC
May 2021

Association Between ApoA1 Gene Polymorphisms and Antipsychotic Drug-Induced Dyslipidemia in Schizophrenia.

Neuropsychiatr Dis Treat 2021 30;17:1289-1297. Epub 2021 Apr 30.

Department of Medical Laboratory, Huai'an Third People's Hospital, Huai'an, People's Republic of China.

Purpose: Dyslipidemia frequently occurs in schizophrenia patients treated with antipsychotic drugs (APDs), especially atypical APDs. Apolipoprotein A1 (ApoA1) plays a key role in lipid metabolism. The aim of this study was to investigate whether ApoA1 gene polymorphisms are associated with APD-induced dyslipidemia in schizophrenia patients.

Patients And Methods: A total of 1987 patients with schizophrenia were enrolled in this study. Serum lipid profiles were determined with a biochemistry analyzer. Genotyping for the rs5072 polymorphism of ApoA1 was performed with TaqMan assay. Logistic regression analysis was carried out to evaluate the relationship between ApoA1 gene polymorphisms and APD-induced dyslipidemia. The effects of drug classification (typical vs atypical APD) and drug regimen (monotherapy vs combination therapy) on serum lipid levels were also analyzed.

Results: A significant association was found between rs5072 and triglyceride (TG) levels in the recessive model of the logistic regression analysis (adjusted odds ratio [OR]=1.50, 95% confidence interval [CI]: 1.03, 2.17; P<0.05). TG level was significantly higher in patients treated with combination therapy (1.03 (0.71, 1.51) mmol/l) compared to monotherapy (0.93 (0.67, 1.43) mmol/l) and was also associated with sex. There were significant differences in TG levels among the three genotypes of ApoA1 rs5072 (GG, GA, and AA) in the whole study population and in patients treated with atypical APDs.

Conclusion: The ApoA1 rs5072 variant is associated with dysregulated TG metabolism in schizophrenia patients treated with APDs, which may increase susceptibility to dyslipidemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/NDT.S305200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096449PMC
April 2021

Oxytocinergic modulation of threat-specific amygdala sensitization in humans is critically mediated by serotonergic mechanisms.

Biol Psychiatry Cogn Neurosci Neuroimaging 2021 Apr 21. Epub 2021 Apr 21.

The Clinical Hospital of the Chengdu Brain Science Institute, School of Life Science and Technology, MOE Key Laboratory for Neuroinformation, University of Electronic Science and Technology of China, 611731 Chengdu, China. Electronic address:

Background: Overarching conceptualizations propose that the complex social-emotional effects of oxytocin (OXT) in humans are partly mediated by interactions with other neurotransmitter systems. Recent animal models suggest that the anxiolytic effects of OXT are critically mediated by the serotonin (5-HT) system, yet direct evidence in humans is lacking.

Methods: To determine the role of 5-HT in OXT-induced attenuation of amygdala threat reactivity and sensitization/ desensitization, we conducted a parallel-group randomized placebo-controlled double-blind experiment during which n = 121 healthy subjects underwent a transient decrease in 5-HT signaling via acute tryptophan depletion (ATD) or the corresponding placebo-control protocols before the administration of intranasal OXT or placebo intranasal spray, respectively. Mean and repetition-dependent changes in threat-specific amygdala reactivity towards threatening stimuli (angry faces) as assessed by fMRI served as the primary outcome.

Results: No treatment main or interaction effects on amygdala threat reactivity were observed, yet OXT switched bilateral amygdala threat sensitization to desensitization and this effect was significantly attenuated during decreased central 5-HT signaling via pretreatment with ATD CONCLUSIONS: The present findings provide the first evidence for a role of OXT in threat-specific amygdala desensitization in humans and suggest that these effects are critically mediated by the 5-HT system. OXT may have a therapeutic potential to facilitate amygdala desensitization and adjunct up-regulation of 5-HT neurotransmission may facilitate OXT's anxiolytic potential.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bpsc.2021.04.009DOI Listing
April 2021

The protective effect of hydrogen-rich water on rats with type 2 diabetes mellitus.

Mol Cell Biochem 2021 Apr 8. Epub 2021 Apr 8.

Key Laboratory of Advanced Technology for Materials of the Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.

The hydrogen-rich water (HW) has been reported to possess a beneficial role in patients with diabetes. However, a systemic evaluation with an appropriate animal model is necessary to reveal its mechanisms and efficacy. Herein, the protective effects of drinking HW on lipid and glucose metabolism, oxidative stress, and inflammation in type 2 diabetes mellitus (T2DM) rats were investigated. The well-modeled T2DM rats (induced by high-fat diet combined with low-dose streptozotocin (STZ) injection) were divided into two groups (n ≥ 15 of each): fed a high-fat diet and drinking distilled water or HW at a constant concentration above 1.0 ppm; normal rats were used as control group (n ≥ 10): fed a regular diet and drinking distilled water. Several biomarkers of lipid and glucose metabolism, oxidative stress ,and inflammation were evaluated after drinking distilled water or HW for 3 weeks. The effect of HW on liver, kidney, and spleen of T2DM rats was also analyzed by HE and Oil Red O staining. The results showed that drinking HW suppressed the increase in glucose, total cholesterol, oxidative stress, and inflammation. Moreover, HW also ameliorates hyperglycemia-induced liver, kidney, and spleen dysfunction. Overall, this study indicates that patients with T2DM may be able to improve their condition by supplementing HW as daily drinking water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11010-021-04145-xDOI Listing
April 2021

A Novel Machine Learning Strategy for the Prediction of Antihypertensive Peptides Derived from Food with High Efficiency.

Foods 2021 Mar 6;10(3). Epub 2021 Mar 6.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Strategies to screen antihypertensive peptides with high throughput and rapid speed will doubtlessly contribute to the treatment of hypertension. Food-derived antihypertensive peptides can reduce blood pressure without side effects. In the present study, a novel model based on the eXtreme Gradient Boosting (XGBoost) algorithm was developed and compared with the dominating machine learning models. To further reflect on the reliability of the method in a real situation, the optimized XGBoost model was utilized to predict the antihypertensive degree of the k-mer peptides cutting from six key proteins in bovine milk, and the peptide-protein docking technology was introduced to verify the findings. The results showed that the XGBoost model achieved outstanding performance, with an accuracy of 86.50% and area under the receiver operating characteristic curve of 94.11%, which were better than the other models. Using the XGBoost model, the prediction of antihypertensive peptides derived from milk protein was consistent with the peptide-protein docking results, and was more efficient. Our results indicate that using the XGBoost algorithm as a novel auxiliary tool is feasible to screen for antihypertensive peptides derived from food, with high throughput and high efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods10030550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999667PMC
March 2021

Diagnostic model for the society safety under COVID-19 pandemic conditions.

Saf Sci 2021 Apr 11;136:105164. Epub 2021 Jan 11.

School of Environment Science and Spatial Informatics, University of Mining and Technology, Xuzhou, Jiangsu 221116, PR China.

The aim of this paper is to develop an information-modeling method for assessing and predicting the consequences of the COVID-19 pandemic. To this end, a detailed analysis of official statistical information provided by global and national organizations is carried out. The developed method is based on the algorithm of multi-channel big data processing considering the demographic and socio-economic information. COVID-19 data are analyzed using an instability indicator and a system of differential equations that describe the dynamics of four groups of people: susceptible, infected, recovered and dead. Indicators of the global sustainable development in various sectors are considered to analyze COVID-19 data. Stochastic processes induced by COVID-19 are assessed with the instability indicator showing the level of stability of official data and the reduction of the level of uncertainty. It turns out that the number of deaths is rising with the Human Development Index. It is revealed that COVID-19 divides the global population into three groups according to the relationship between Gross Domestic Product and the number of infected people. The prognosis for the number of infected people in December 2020 and January-February 2021 shows negative events which will decrease slowly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ssci.2021.105164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972928PMC
April 2021

Variations of Time Irreversibility of Heart Rate Variability Under Normobaric Hypoxic Exposure.

Front Physiol 2021 5;12:607356. Epub 2021 Mar 5.

School of Instrument Science and Engineering, Southeast University, Nanjing, China.

In the field of biomedicine, time irreversibility is used to describe how imbalanced and asymmetric biological signals are. As an important feature of signals, the direction of time is always ignored. To find out the variation regularity of time irreversibility of heart rate variability (HRV) in the initial stage of hypoxic exposure, the present study implemented 2 h acute normobaric hypoxic exposure on six young subjects who have no plateau or hypoxia experiences; oxygen concentration was set as 12.9%. Electrocardiogram (ECG) signals were recorded in the whole process and RR interval sequences were extracted. Mathematical operations were executed to transform the difference of adjacent RR intervals into proportion and distance with delay time to conduct time irreversibility analysis of HRV. The same calculating method was implemented on six items randomly picked out from the MIT-BIH normal sinus rhythm database as a control group. Results show that variation of time irreversibility of HRV in a hypoxic environment is different from that in a normoxic environment, time irreversibility indices of a hypoxic group decreases continually at a delay time of 1 and 2, and indices curves of time irreversibility gradually tend to be steady and gather with each other at a delay time of 3 or 4. The control group shows no consistent tendency no matter what the delay time is in the range of 1-4. Our study indicates that in short-time hypoxic exposure, as hypoxic time goes by, regulation of the cardiovascular autonomic nervous system weakens; regulation times and intensity of sympathetic and parasympathetic nerves tend to be equal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2021.607356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973363PMC
March 2021

Coproduction of hydrogen and lactic acid from glucose photocatalysis on band-engineered ZnCdS homojunction.

iScience 2021 Feb 28;24(2):102109. Epub 2021 Jan 28.

Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Drive, NW, Calgary, Alberta T2N 1N4, Canada.

Photocatalytic transformation of biomass into value-added chemicals coupled with co-production of hydrogen provides an explicit route to trap sunlight into the chemical bonds. Here, we demonstrate a rational design of ZnCdS solid solution homojunction photocatalyst with a pseudo-periodic cubic zinc blende (ZB) and hexagonal wurtzite (WZ) structure for efficient glucose conversion to simultaneously produce hydrogen and lactic acid. The optimized ZnCdS catalyst consists of a twinning superlattice, has a tuned bandgap, and displays excellent efficiency with respect to hydrogen generation (690 ± 27.6 μmol·h·g), glucose conversion (~90%), and lactic acid selectivity (~87%) without any co-catalyst under visible light irradiation. The periodic WZ/ZB phase in twinning superlattice facilitates better charge separation, while superoxide radical (⋅O) and photogenerated holes drive the glucose transformation and water oxidation reactions, respectively. This work demonstrates that rational photocatalyst design could realize an efficient and concomitant production of hydrogen and value-added chemicals from glucose photocatalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2021.102109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881236PMC
February 2021

Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22.

Appl Biochem Biotechnol 2021 Feb 19. Epub 2021 Feb 19.

College of Energy, Xiamen University, Xiamen, 361102, China.

Trichoderma orientalis (T. orientalis) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in Trichoderma reesei (T. reesei). However, the ace2 gene cannot be found in the genome of T. orientalis. Researches show that the mechanism of cellulase transcriptional regulation in T. orientalis keeps high similarity with T. reesei up till now. So, in this study, the ace2 of Trichoderma reesei QM9414 was heterologous expressed in T. orientalis EU7-22. As a result, xylanase activity and β-glucosidase activity of ace2 heterogeneous expression strains are improved and total cellulase activity is decreased. The result of qPCR is in accordance with enzyme activities. This study provides a reference for an in-depth study on transcriptional regulation mechanisms of T. orientalis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-021-03529-5DOI Listing
February 2021

Genome-wide identification, characterisation, and evolution of subfamily in nine Rosaceae species and expression analysis in mei ().

PeerJ 2021 4;9:e10785. Epub 2021 Feb 4.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.

Rosaceae is an important family containing some of the highly evolved fruit and ornamental plants. Abiotic stress responses play key roles in the seasonal growth and development of plants. However, the molecular basis of stress responses remains largely unknown in Rosaceae. Abscisic acid (ABA) is a stress hormone involving abiotic stress response pathways. The ABRE-binding factor/ABA-responsive element-binding protein (ABF/AREB) is a subfamily of the basic domain/leucine zipper (bZIP) transcription factor family. It plays an important role in the ABA-mediated signaling pathway. Here, we analyzed the subfamily genes in nine Rosaceae species. A total of 64 genes were identified, including 18, 28, and 18 genes in the Rosoideae, Amygdaloideae, and Maloideae traditional subfamilies, respectively. The evolutionary relationship of the subfamily genes was studied through the phylogenetic analysis, the gene structure and conserved motif composition, Ka/Ks values, and interspecies colinearity. These gene sets were clustered into four groups. In the () promoters, several -elements related to light, hormone, and abiotic stress response were predicted. expressed in five different tissues, except , which expressed only in buds. In the dormancy stages, , , and showed differential expression. The expression of , and was positively correlated with the ABA concentration. Except for , all the were sensitive to ABA. Several ABRE elements were contained in the promoters of , , , . Based on the findings of our study, we speculate that may play a role in flower bud dormancy in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.10785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868070PMC
February 2021

Extraction, purification, bioactivity and pharmacological effects of capsaicin: a review.

Crit Rev Food Sci Nutr 2021 Feb 16:1-29. Epub 2021 Feb 16.

College of Food Science & Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetable Processing, Ministry of Agricultural and Rural Affairs, China Agricultural University, Beijing, China.

Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), a well-known vanilloid, which is the main spicy component in chili peppers, showing several biological activities and the potential applications range from food flavorings to therapeutics. Traditional extraction of capsaicin by organic solvents was time-consuming, some new methods such as aqueous two-phase method and ionic liquid extraction method have been developed. During past few decades, an ample variety of biological effects of capsaicin have been evaluated. Capsaicin can be used in biofilms and antifouling coatings due to its antimicrobial activity, allowing it has a promising application in food packaging, food preservation, marine environment and dental therapy. Capsaicin also play a crucial role in metabolic disorders, including weight loss, pressure lowing and insulin reduction effects. In addition, capsaicin was identified effective on preventing human cancers, such as lung cancer, stomach cancer, colon cancer and breast cancer by inducing apoptosis and inhibiting cell proliferation of tumor cells. Previous research also suggest the positive effects of capsaicin on pain relief and cognitive impairment. Capsaicin, the agonist of transient receptor potential vanilloid type 1 (TRPV1), could selectively activate TRPV1, inducing Ca influx and related signaling pathways. Recently, gut microbiota was also involved in some diseases therapeutics, but its influence on the effects of capsaicin still need to be deeply studied. In this review, different extraction and purification methods of capsaicin, its biological activities and pharmacological effects were systematically summarized, as well as the possible mechanisms were also deeply discussed. This article will give an updated and better understanding of capsaicin-related biological effects and provide theoretical basis for its further research and applications in human health and manufacture development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10408398.2021.1884840DOI Listing
February 2021

Protein Isolates from Raw and Cooked Foxtail Millet Attenuate Development of Type 2 Diabetes in Streptozotocin-Induced Diabetic Mice.

Mol Nutr Food Res 2021 03 22;65(6):e2000365. Epub 2021 Jan 22.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.

Scope: Millet protein has received much attention due to its beneficial role in alleviating metabolic disease symptoms. This study aims to investigate the role and molecular mechanism of foxtail millet protein isolates, including protein isolates from raw and cooked foxtail millet in alleviating diabetes, including gut microbiota and intracellular signal pathways.

Methods And Results: Protein isolates from raw and cooked foxtail millet are orally administered to streptozotocin (STZ)-induced diabetic mice for 5 weeks before hypoglycemic effect evaluation. The results show that foxtail millet protein isolates improve glucose intolerance and insulin resistance in diabetic mice. However, only the protein isolate from cooked foxtail millet reverse the weight loss trend and alleviate lipid disorders in diabetic mice. Besides, 16S rRNA sequencing show that both raw and cooked foxtail millet protein isolates altered diabetes-induced gut dysbiosis. In addition, western blotting analysis indicated that the protein isolate from cooked foxtail millet increases the expression levels of glucagon-like peptide-1 receptor (GLP-1R), phosphoinositide 3-kinase (PI3K), and phosphoinositide-protein kinase B (p-AKT)/AKT while the protein isolate from raw foxtail millet downregulates stearoyl-coenzyme A desaturase 1 (SCD1) level.

Conclusion: Both raw and cooked foxtail millet protein isolates can exert hypoglycemic effects in diabetic mice through rewiring glucose homeostasis, mitigating diabetes-induced gut dysbiosis, and affecting the GLP-1R/PI3K/AKT pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mnfr.202000365DOI Listing
March 2021

The Effects of Earthworms on Fungal Diversity and Community Structure in Farmland Soil With Returned Straw.

Front Microbiol 2020 17;11:594265. Epub 2020 Dec 17.

Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Background: To promote the decomposition of returned straw, reduce the incidence of soil-borne diseases caused by returned straw, and accelerate the conversion of straw carbon into soil carbon, we inoculated earthworms into fields with returned straw. The earthworms accelerated straw degradation and promoted carbon conversion. However, the impact of externally inoculated earthworms on the farmland soil ecosystem, especially the structure and the function of its microbial community, remains unclear.

Methods: We analyzed the effects of straw return and earthworms on the diversity of fungal populations and the community structure of dominant fungal taxa in soil by quantifying fungal population size and community composition PCR amplification of internal transcribed spacer genes and 18S rRNA gene sequencing.

Results: The results showed that earthworm inoculation significantly accelerated the degradation of rice straw and promoted the conversion of straw carbon to soil carbon. Both fungal abundance and α-diversity (Sobs and Shannon indices) were higher in the plots with surface straw but without earthworms than in those inoculated with earthworms and in the CK. Principal component analysis indicated that straw return increased the diversity and the abundance of the fungal community, whereas earthworms inhibited this expansion of the fungal community caused by straw return. Interestingly, the overall differences in fungal community composition were smallest in plots with straw return, while the dominant fungal community features in plots inoculated with earthworms were closer to those of the CK.

Conclusion: Generally, straw return stimulated unclassified_K_fungi, , and with strong cellulolytic ability. In contrast, the abundances of , unclassified_c_Sordariomycetes, unclassified_f_Lasiosphaeriaceae, and were higher in the plots inoculated with earthworms and in the CK. Furthermore, evolutionary analysis showed that the evolution of soil fungal communities tended to diverge after straw return, and the evolutionary directions of fungal species in the plots inoculated with earthworms were similar to those in the CK.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.594265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773728PMC
December 2020

Remote Sensing Evaluation of Total Suspended Solids Dynamic with Markov Model: A Case Study of Inland Reservoir across Administrative Boundary in South China.

Sensors (Basel) 2020 Dec 3;20(23). Epub 2020 Dec 3.

College of Engineering and Technology, University of Derby, Derby DE22 1GB, UK.

Accurate and quantitative assessment of the impact of natural environmental changes and human activities on total suspended solids (TSS) concentration is one of the important components of water environment protection. Due to the limits of traditional cross-sectional point monitoring, a novel water quality evaluation method based on the Markov model and remote sensing retrieval is proposed to realize the innovation of large-scale spatial monitoring across administrative boundaries. Additionally, to explore the spatiotemporal characteristics and driving factors of TSS, a new three-band remote sensing model of TSS was built by regression analysis for the inland reservoir using the synchronous field spectral data, water quality samples and remote sensing data in the trans-provincial Hedi Reservoir in the Guangdong and Guangxi Provinces of South China. The results show that: (1) The three-band model based on the OLI sensor explained about 82% of the TSS concentration variation (R2=0.81, N=34,  p value<0.01) with an acceptable validation accuracy (RMSE=6.24 mg/L,MRE=18.02%, N=15), which is basically the first model of its kind available in South China. (2) The TSS concentration has spatial distribution characteristics of high upstream and low downstream, where the average TSS at 31.54 mg/L in the upstream are 2.5 times those of the downstream (12.55 mg/L). (3) Different seasons and rainfall are important factors affecting the TSS in the upstream cross-border area, the TSS in the dry season are higher with average TSS of 33.66 mg/L and TSS are negatively correlated with rainfall from upstream mankind activity. Generally, TSS are higher in rainy seasons than those in dry seasons. However, the result shows that TSS are negatively correlated with rainfall, which means human activities have higher impacts on water quality than climate change. (4) The Markov dynamic evaluation results show that the water quality improvement in the upstream Shijiao Town is the most obvious, especially in 2018, the improvement in the water quality level crossed three levels and the TSS were the lowest. This study provided a technical method for remote sensing dynamic monitoring of water quality in a large reservoir, which is of great significance for remediation of the water environment and the effective evaluation of the river and lake chief system in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20236911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730694PMC
December 2020

Distinct roles of nucleosome sliding and histone modifications in controlling the fidelity of transcription initiation.

RNA Biol 2021 Jan 28:1-11. Epub 2021 Jan 28.

Department of Biology, Saint Louis University, St. Louis, Missouri, USA.

Regulation of gene expression starts from the transcription initiation. Regulated transcription initiation is critical for generating correct transcripts with proper abundance. The impact of epigenetic control, such as histone modifications and chromatin remodelling, on gene regulation has been extensively investigated, but their specific role in regulating transcription initiation is far from well understood. Here we aimed to better understand the roles of genes involved in histone H3 methylations and chromatin remodelling on the regulation of transcription initiation at a genome-scale using the budding yeast as a study system. We obtained and compared maps of transcription start site (TSS) at single-nucleotide resolution by nAnT-iCAGE for a strain with depletion of MINC (Mot1-Ino80C-Nc2) by Mot1p and Ino80p anchor-away () and a strain with loss of histone methylation () to their wild-type controls. Our study showed that the depletion of MINC stimulated transcription initiation from many new sites flanking the dominant TSS of genes, while the loss of histone methylation generates more TSSs in the coding region. Moreover, the depletion of MINC led to less confined boundaries of TSS clusters (TCs) and resulted in broader core promoters, and such patterns are not present in the mutant. Our data also exhibits that the MINC has distinctive impacts on TATA-containing and TATA-less promoters. In conclusion, our study shows that MINC is required for accurate identification of bona fide TSSs, particularly in TATA-containing promoters, and histone methylation contributes to the repression of transcription initiation in coding regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15476286.2020.1860389DOI Listing
January 2021

Hypoglycemic Effect of Prolamin from Cooked Foxtail Millet () on Streptozotocin-Induced Diabetic Mice.

Nutrients 2020 Nov 11;12(11). Epub 2020 Nov 11.

Key Laboratory of Plant Protein and Grain Processing, National Engineering Research Center for Fruits and Vegetables Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Millet proteins have been demonstrated to possess glucose-lowering and lipid metabolic disorder modulation functions against diabetes; however, the molecular mechanisms underlying their anti-diabetic effects remain unclear. The present study aimed to investigate the hypoglycemic effect of prolamin from cooked foxtail millet (PCFM) on type 2 diabetic mice, and explore the gut microbiota and serum metabolic profile changes that are associated with diabetes attenuation by PCFM. Our diabetes model was established using a high-fat diet combined with streptozotocin before PCFM or saline was daily administrated by gavage for 5 weeks. The results showed that PCFM ameliorated glucose metabolism disorders associated with type 2 diabetes. Furthermore, the effects of PCFM administration on gut microbiota and serum metabolome were investigated. 16S rRNA gene sequencing analysis indicated that PCFM alleviated diabetes-related gut microbiota dysbiosis in mice. Additionally, the serum metabolomics analysis revealed that the metabolite levels disturbed by diabetes were partly altered by PCFM. Notably, the decreased D-Glucose level caused by PCFM suggested that its anti-diabetic potential can be associated with the activation of glycolysis and the inhibition of gluconeogenesis, starch and sucrose metabolism and galactose metabolism. In addition, the increased serotonin level caused by PCFM may stimulate insulin secretion by pancreatic β-cells, which contributed to its hypoglycemic effect. Taken together, our research demonstrated that the modulation of gut microbiota composition and the serum metabolomics profile was associated with the anti-diabetic effect of PCFM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu12113452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696583PMC
November 2020

Beneficial effects of mung bean seed coat on the prevention of high-fat diet-induced obesity and the modulation of gut microbiota in mice.

Eur J Nutr 2021 Jun 1;60(4):2029-2045. Epub 2020 Oct 1.

College of Food Science and Nutritional Engineering, China Agricultural University, No.17, Qinghua East Road, Haidian District, Beijing, 100083, China.

Purpose: Our recent study has reported that whole mung bean showed better beneficial effects on high-fat diet (HFD)-induced obesity and gut microbiota disorders when compared with the decorticated mung bean at the same intervention dose level, suggesting that the mung bean seed coat (MBC) may play a crucial role in its health benefits. This study aims to investigate whether MBC has beneficial benefits on the prevention of HFD-induced obesity and the modulation of gut microbiota in mice when it was supplemented in HFD.

Methods: Herein, male C57BL/6 J mice were fed with normal control diet, HFD, and HFD supplemented with MBC (3-6%, w/w) for 12 weeks. The changes in physiological, histological, biochemical parameters, serum endotoxin, proinflammatory cytokines, and gut microbiota composition of mice were determined to assess the ability of MBC to alleviate HFD-induced obesity and modulate gut microbiota disorders in mice.

Results: MBC supplementation exhibited significant reductions in the HFD-induced adiposity, fat accumulation, serum lipid levels, lipopolysaccharide, and proinflammatory cytokines concentrations (P < 0.05), which was accompanied by improvements in hepatic steatosis and adipocyte size. Especially, the elevated fasting blood glucose and insulin resistance were also significantly improved by MBC supplementation (P < 0.05). Furthermore, high-throughput sequencing of the 16S rRNA gene revealed that MBC could normalize HFD-induced gut microbiota dysbiosis. MBC not only could promote the bloom of Akkermansia, but also restore several HFD-dependent taxa (Blautia, Ruminiclostridium_9, Bilophila, and unclassified_f_Ruminococcaceae) back to normal status, co-occurring with the decreases in obesity-related indices.

Conclusions: This study provides evidence that MBC may be mainly responsible for the beneficial effects of whole mung bean on preventing the HFD-induced changes, thus enlarging the application value of MBC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00394-020-02395-xDOI Listing
June 2021

Earthworms accelerate rice straw decomposition and maintenance of soil organic carbon dynamics in rice agroecosystems.

PeerJ 2020 17;8:e9870. Epub 2020 Sep 17.

Institute of Eco-Environmental and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Background: To promote straw degradation, we inoculated returned farmland straw with earthworms (). Increasing the number of earthworms may generally alter soil organic carbon (SOC) dynamics and the biological activity of agricultural soils.

Methods: We performed soil mesocosm experiments with and without earthworms to assess the decomposition and microbial mineralization of returned straw and soil enzyme activity across different time periods.

Results: When earthworms were present in soil, the surface residues were completely consumed during the first four weeks, but when earthworms were absent, most of the residues remained on the soil surface after 18 weeks. On day 28, the SOC content was significantly higher in the treatment where both earthworms and residue had been added. The SOC content was lower in the treatment where earthworms but no residue had been added. The organic carbon content in water-stable macroaggregates showed the same trend. During the first 14 weeks, the soil basal respiration was highest in the treatments with both residues and earthworms. From weeks 14 to 18, basal respiration was highest in the treatments with residues but without earthworms. We found a significant positive correlation between soil basal respiration and soil dissolved organic carbon content. Earthworms increased the activity of protease, invertase, urease and alkaline phosphatase enzymes, but decreased β-cellobiohydrolase, β-glucosidase and xylosidase activity, as well as significantly reducing ergosterol content.

Conclusion: The primary decomposition of exogenous rice residues was mainly performed by earthworms. Over a short period of time, they converted plant carbon into soil carbon and increased SOC. The earthworms played a key role in carbon conversion and stabilization. In the absence of exogenous residues, earthworm activity accelerated the decomposition of original organic carbon in the soil, reduced SOC, and promoted carbon mineralization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.9870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502234PMC
September 2020

Perturbations of gut microbiota in gestational diabetes mellitus patients induce hyperglycemia in germ-free mice.

J Dev Orig Health Dis 2020 12 14;11(6):580-588. Epub 2020 Sep 14.

Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China.

Shifts in the maternal gut microbiota have been implicated in the development of gestational diabetes mellitus (GDM). Understanding the interaction between gut microbiota and host glucose metabolism will provide a new target of prediction and treatment. In this nested case-control study, we aimed to investigate the causal effects of gut microbiota from GDM patients on the glucose metabolism of germ-free (GF) mice. Stool and peripheral blood samples, as well as clinical information, were collected from 45 GDM patients and 45 healthy controls (matched by age and prepregnancy body mass index (BMI)) in the first and second trimester. Gut microbiota profiles were explored by next-generation sequencing of the 16S rRNA gene, and inflammatory factors in peripheral blood were analyzed by enzyme-linked immunosorbent assay. Fecal samples from GDM and non-GDM donors were transferred to GF mice. The gut microbiota of women with GDM showed reduced richness, specifically decreased Bacteroides and Akkermansia, as well as increased Faecalibacterium. The relative abundance of Akkermansia was negatively associated with blood glucose levels, and the relative abundance of Faecalibacterium was positively related to inflammatory factor concentrations. The transfer of fecal microbiota from GDM and non-GDM donors to GF mice resulted in different gut microbiota colonization patterns, and hyperglycemia was induced in mice that received GDM donor microbiota. These results suggested that the shifting pattern of gut microbiota in GDM patients contributed to disease pathogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S2040174420000768DOI Listing
December 2020

INO80C Remodeler Maintains Genomic Stability by Preventing Promiscuous Transcription at Replication Origins.

Cell Rep 2020 09;32(10):108106

Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA. Electronic address:

The proper coordination of transcription with DNA replication and repair is central for genomic stability. We investigate how the INO80C chromatin remodeling enzyme might coordinate these genomic processes. We find that INO80C co-localizes with the origin recognition complex (ORC) at yeast replication origins and is bound to replication initiation sites in mouse embryonic stem cells (mESCs). In yeast, INO80C recruitment requires origin sequences but does not require ORC, suggesting that recruitment is independent of pre-replication complex assembly. In both yeast and ESCs, INO80C co-localizes at origins with Mot1 and NC2 transcription factors, and genetic studies suggest that they function together to promote genome stability. Interestingly, nascent transcript sequencing demonstrates that INO80C and Mot1 prevent pervasive transcription through origin sequences, and absence of these factors leads to formation of new DNA double-strand breaks. We propose that INO80C and Mot1/NC2 function through distinct pathways to limit origin transcription, maintaining genomic stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2020.108106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540730PMC
September 2020

Humanistic care and psychological counseling on psychological disorders in medical students after COVID-19 outbreak: A protocol of systematic review.

Medicine (Baltimore) 2020 Aug;99(33):e21484

First Ward of Orthopedics Department, First Affiliated Hospital of Jiamusi University, Jiamusi, China.

Background: The objective of this study is to investigate the effects of humanistic care and psychological counseling (HCPC) on psychological disorders (PD) in medical students after coronavirus disease 2019 (COVID-19) outbreak.

Methods: We will search randomized controlled trials or case-controlled studies of HCPC on PD in medical students after COVID-19 outbreak in the following electronic databases: PUBMED/MEDLINE, EMBASE, Cochrane Library, CINAHL, AMED, WANGFANG, and CNKI. The time is restricted from the construction of each database to the present. All process of study selection, data collection, and study quality evaluation will be carried out by two independent authors. Any different opinions will be solved by a third author through discussion. We will employ RevMan 5.3 software to conduct statistical analysis.

Results: This study will provide a better understanding of HCPC on PD in medical students after COVID-19 outbreak.

Conclusions: This study may offer strong evidence for clinical practice to treat PD in medical students after COVID-19 outbreak.

Study Registration: CRD42020193199.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000021484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437756PMC
August 2020

Microstructure and Texture Evolution of AZ31 Alloy Prepared by Cyclic Expansion Extrusion with Asymmetrical Extrusion Cavity at Different Temperatures.

Materials (Basel) 2020 Aug 25;13(17). Epub 2020 Aug 25.

School of Materials Science and Engineering, North University of China, Taiyuan 030051, China.

This work is to study the microstructure and texture evolution of AZ31 alloy prepared by cyclic expansion extrusion with an asymmetrical extrusion cavity (CEE-AEC) at different deformation temperatures. The result shows AZ31 alloy undergoes continuous dynamic recrystallization (CDRX) and discontinuous dynamic recrystallization (DDRX) during CEE-AEC processing. At the initial stage of deformation, AZ31 alloys exhibit similar bimodal microstructure of coarse deformed grains surrounded by fine DRXed grains. As the passes increase, the cumulative strain increases, and the coarse grains of all samples are almost replaced by fine equiaxed grains. The average grain sizes and the basal texture intensities of the deformed samples increase as the deformation temperature increases. In addition, due to the existence of an asymmetrical cavity, as the passes increase, the basal textures of all samples are deflected with maximum intensities increase, and even an unusual bimodal texture is formed, resulting in a soft orientation that is easy to basal slip.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13173757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504158PMC
August 2020

In Vitro Starch Digestibility and Estimated Glycemic Index of Mung Bean (Vigna radiata L.) as Affected by Endogenous Proteins and Lipids, and Exogenous Heat-Processing Methods.

Plant Foods Hum Nutr 2020 Dec;75(4):547-552

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.

The aim of this study was to investigate the in vitro starch digestibility and estimated glycemic index (eGI) of mung bean (Vigna radiata L.) as affected by endogenous proteins and lipids, and exogenous heat-processing methods. Results showed that the in vitro starch digestibility and eGI were significantly increased after the removal of protein, lipid, or both (P < 0.05). Moreover, the effects of endogenous proteins and lipids on the in vitro starch digestibility and eGI of mung bean followed the order: both protein and lipid removal > protein removal > lipid removal. In addition, heat-processing could make the slowly digestible starch and resistant starch convert to the rapidly digestible starch. The effects of different exogenous heat-processing methods on in vitro starch digestibility and eGI in the present study showed the following order: germination combined with cooking > high-pressure cooking > extrusion cooking > ordinary cooking. This study provided a crucial reference for the human with potential hyperglycemia to choose mung bean treated with ordinary cooking and without further protein and/or lipid removal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11130-020-00845-9DOI Listing
December 2020