Publications by authors named "Yong Sun"

751 Publications

Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug.

Int Immunopharmacol 2021 Oct 7;101(Pt A):108166. Epub 2021 Oct 7.

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. Electronic address:

Our previous studies demonstrated that Curc-mPEG454, a curcumin derivative modified with short-chain polyethylene glycol (PEG), not only increased the blood concentration of curcumin, but also retained its anti-inflammatory activity. Here, we aimed to evaluate the anti-fibrotic effect of Curc-mPEG454 on a rat liver fibrosis model induced by carbon tetrachloride (CCl), and to explore the underlying mechanisms by integrating our total liver RNA sequencing (RNA-seq) data with recent liver single-cell sequencing (scRNA-seq) studies. 50 mg/kg and 100 mg/kg Curc-mPEG454 treatment significantly reduced the elevation of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) induced by CCl, and the incidence of liver cirrhosis decreased from 75% to 37% and 35%, respectively. RNA-seq analysis revealed that Curc-mPEG454 significantly upregulated aldehyde oxidase 1 (AOX1) while downregulated cytochrome p450 26A1 (CYP26A1) and cytochrome p450 26B1 (CYP26B1) resulting in restoring liver retinoic acid (RA) level, increased glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) expression to synthesize hepatic glutathione (GSH), and inhibited liver inflammation via down-regulating the Prostaglandin E Synthase 2 (PTGES2)/prostacyclin E2 (PGE2) signaling. Integrating scRNA-seq data revealed that Curc-mPEG454 effectively inhibited the expansion of scar-associated macrophage subpopulation and scar-producing myofibroblasts in the damaged liver, and remodeled the fibrotic niche via regulation of ligand-receptor interactions including platelet-derived growth factor-B (PDGF-B)/platelet-derived growth factor receptor-α (PDGFR-α) signaling. As a multi-target prodrug, PEGylated curcumin deserves further attention and research.
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http://dx.doi.org/10.1016/j.intimp.2021.108166DOI Listing
October 2021

Bifunctional alginate/chitosan stabilized perfluorohexane nanodroplets as smart vehicles for ultrasound and pH responsive delivery of anticancer agents.

Int J Biol Macromol 2021 Sep 30. Epub 2021 Sep 30.

Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory School of Medicine, Atlanta, GA 30332, USA.

The combination of ultrasound and chemotherapy has been proposed as a promising strategy to achieve a better anticancer therapeutic efficacy. Here we present a facile strategy to construct novel bifunctional nanodroplets as smart vehicles for ultrasound and pH responsive delivery of anticancer agents. PFH is used as core and chitosan/alginate complexes are used as the stable shells of the nanodroplets. The effects of alginate/chitosan ratio, and the amount of surfactant as well as PFH on the size, size distribution, and encapsulation efficiency of nanodroplets are systematically investigated with the optimized formulation identified. The release of the encapsulated doxorubicin hydrochloride can be triggered by changing the pH value of the surrounding environment and the exposure to ultrasound. The nanodroplets also show strong ultrasound contrast via droplet-to-bubble transition as demonstrated by B-mode ultrasound imaging. The hemolytic activity and cytotoxicity are further studied, revealing the biocompatibility of the nanodroplets. The in vivo antitumor results demonstrate that the prepared droplets show excellent antitumor therapeutic efficacy and outstanding tumor-targeting ability. The proposed alginate/chitosan stabilized PFH nanodroplets represent an important advance in fabricating multifunctional therapeutic materials with great promises in the applications of combined antitumor therapies.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.166DOI Listing
September 2021

Metabolism of Phenolics of Roots under In Vitro Digestion and Colonic Fermentation as Well as Their In Vivo Antioxidant Activity in Rats.

Foods 2021 Sep 8;10(9). Epub 2021 Sep 8.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

Diels et Gilg is a herbaceous perennial species distributed mainly in southern China. The root (THR) has been prevalently consumed as a functional tea or dietary supplement. In vitro digestion models, including colonic fermentation, were built to evaluate the release and stability of THR phenolics with the method of HPLC-QqQ-MS/MS and UPLC-Qtof-MS/MS. From the oral cavity, the contents of total phenolic and flavonoid began to degrade. Quercetin-3-rutinoside, quercetin-3-glucoside, kaempferol-3-rutinoside, and kaempferol-3-glucoside were metabolized as major components and they were absorbed in the form of glycosides for hepatic metabolism. On the other hand, the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH) content were significantly increased, while malondialdehyde (MDA) content was decreased in plasma and tissues of rats treated with THR extract in the oxidative stress model. These results indicated that the THR extract is a good antioxidant substance and has good bioavailability, which can effectively prevent some chronic diseases caused by oxidative stress. It also provides a basis for the effectiveness of THR as a traditional functional food.
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http://dx.doi.org/10.3390/foods10092123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470164PMC
September 2021

Current progress on catalytic oxidation of toluene: a review.

Environ Sci Pollut Res Int 2021 Sep 27. Epub 2021 Sep 27.

Department of Chemical and Environmental Engineering, University of Nottingham Ningbo China, Ningbo, People's Republic of China.

Toluene is one of the pollutants that are dangerous to the environment and human health and has been sorted into priority pollutants; hence, the control of its emission is necessary. Due to severe problems caused by toluene, different techniques for the abatement of toluene have been developed. Catalytic oxidation is one of the promising methods and effective technologies for toluene degradation as it oxidizes it to CO and does not deliver other pollutants to the environment. This paper highlights the recent progressive advancement of the catalysts for toluene oxidation. Five categories of catalysts, including noble metal catalysts, transition metal catalysts, perovskite catalysts, metal-organic frameworks (MOFs)-based catalysts, and spinel catalysts reported in the past half a decade (2015-2020), are reviewed. Various factors that influence their catalytic activities, such as morphology and structure, preparation methods, specific surface area, relative humidity, and coke formation, are discussed. Furthermore, the reaction mechanisms and kinetics for catalytic oxidation of toluene are also discussed.
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http://dx.doi.org/10.1007/s11356-021-16492-9DOI Listing
September 2021

New Xanthones with Antiagricultural Fungal Pathogen Activities from the Endophytic Fungus L17.

J Agric Food Chem 2021 Sep 19;69(38):11216-11224. Epub 2021 Sep 19.

Department of Natural Medicinal Chemistry and Pharmacognosy, School of Pharmacy, Qingdao University, Qingdao, Shandong 266021, People's Republic of China.

Six new xanthone dimers, diaporxanthones A-F (-), and an unusual xanthone monomer diaporxanthone G (), in addition to seven known analogues (-), were isolated and identified from endophytic L17 harbored in the fruits of the salt-tolerant plant . The chemical structures of these metabolites were elucidated on the basis of nuclear magnetic resonance, high-resolution electrospray ionization mass spectrometry, and reported data in the literature. Their absolute configurations were established by single-crystal X-ray diffraction analysis together with time-dependent density functional theory electronic circular dichroism calculations. Among these compounds, compounds and exhibited moderate antifungal activities against sp. and and compound showed significant cytotoxicity against all selected five cancer cell lines.
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http://dx.doi.org/10.1021/acs.jafc.1c03513DOI Listing
September 2021

Bioinspired supramolecular nanofiber hydrogel through self-assembly of biphenyl-tripeptide for tissue engineering.

Bioact Mater 2022 Feb 11;8:396-408. Epub 2021 Jun 11.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan, 610064, PR China.

Supramolecular nanofiber peptide assemblies had been used to construct functional hydrogel biomaterials and achieved great progress. Here, a new class of biphenyl-tripeptides with different C-terminal amino acids sequences transposition were developed, which could self-assemble to form robust supramolecular nanofiber hydrogels from 0.7 to 13.8 kPa at ultra-low weight percent (about 0.27 wt%). Using molecular dynamics simulations to interrogate the physicochemical properties of designed biphenyl-tripeptide sequences in atomic detail, reasonable hydrogen bond interactions and "FF" brick (phenylalanine-phenylalanine) promoted the formation of supramolecular fibrous hydrogels. The biomechanical properties and intermolecular interactions were also analyzed by rheology and spectroscopy analysis to optimize amino acid sequence. Enhanced L929 cells adhesion and proliferation demonstrated good biocompatibility of the hydrogels. The storage modulus of BPAA-AFF with 10 nm nanofibers self-assembling was around 13.8 kPa, and the morphology was similar to natural extracellular matrix. These supramolecular nanofiber hydrogels could effectively support chondrocytes spreading and proliferation, and specifically enhance chondrogenic related genes expression and chondrogenic matrix secretion. Such biomimetic supramolecular short peptide biomaterials hold great potential in regenerative medicine as promising innovative matrices because of their simple and regular molecular structure and excellent biological performance.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429915PMC
February 2022

Anaerobic digestion of corn straw pretreated by ultrasonic combined with aerobic hydrolysis.

Bioresour Technol 2021 Dec 21;341:125826. Epub 2021 Aug 21.

College of engineering Northeast Agriculture University, Harbin 15000, PR China. Electronic address:

Corn straw (CS) was pretreated by ultrasonic combined aerobic with biogas slurry as medium for anaerobic digestion (AD), that strengthened the degradation efficiency CS, varied in the composition of digestion slurry, thereby the methane production was increased. Central combinatorial design (CCD) test was used to treat CS at ultrasonic power (200, 400, and 600 W), time (10, 20, and 30 min) and AD for 25 days, at 37 ± 1℃. According to data showed that the pH and volatile fatty acids (VFAs) affected methane production directly. With an ultrasonic power 309 W, time 26 min, it reached the maximum content of VFAs with 16.24 g/L, the cumulative methane production achieved the highest with 198.56 mL/g VS, which was 46.73% higher than unpretreated raw material as CK. Ultrasonic-aerobic hydrolysis pretreatment can obtain higher VFAs and methane production content in a short period of time that is great significance to biogas engineering.
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http://dx.doi.org/10.1016/j.biortech.2021.125826DOI Listing
December 2021

Unveiling the catalytic ability of carbonaceous materials in Fenton-like reaction by controlled-release CaO nanoparticles for trichloroethylene degradation.

J Hazard Mater 2021 08 24;416:125935. Epub 2021 Apr 24.

State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Carbonaceous materials (CMs) have been applied extensively for enhancing the catalytic performance of environmental catalysts, however, the self-catalytic mechanism of CMs for groundwater remediation is rarely investigated. Herein, we unveiled the catalytic ability of various CMs via Fe(III) reduction through polyvinyl alcohol-coated calcium peroxide nanoparticles ([email protected]) for trichloroethylene (TCE) removal. Among selected CMs (graphite (G), biochar (BC) and activated carbon (AC)), BC and AC showed enhancement of TCE removal of 89% and 98% via both adsorption and catalytic degradation. BET and SEM analyses showed a higher adsorption capacity of AC (27.8%) than others. The generation of solution-Fe(II) and surface-Fe(II) revealed the reduction of Fe(III) on CMs-surface. The role of O-containing groups was investigated by the FTIR technique and XPS quantified the 52% and 57% surface-Fe(II) in BC and AC systems, respectively. EPR and quenching tests confirmed that both solution and surface-bound species (HO•, O• and O) contributed to TCE degradation. Acidic pH condition encouraged TCE removal and the presence of HCO negatively affected TCE removal than other inorganic ions. Both schemes ([email protected]/Fe(III)/BC and [email protected]/Fe(III)/AC) exhibited promising results in the actual groundwater, surfactant-amended solution, and removal of other chlorinated-pollutants, opening a new direction towards green environmental remediation for prolonged benefits.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125935DOI Listing
August 2021

Crucial Mutations of Spike Protein on SARS-CoV-2 Evolved to Variant Strains Escaping Neutralization of Convalescent Plasmas and RBD-Specific Monoclonal Antibodies.

Front Immunol 2021 17;12:693775. Epub 2021 Aug 17.

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), Hefei, China.

Small number of SARS-CoV-2 epidemic lineages did not efficiently exhibit a neutralization profile, while single amino acid mutation in the spike protein has not been confirmed in altering viral antigenicity resulting in immune escape. To identify crucial mutations in spike protein that escape humoral immune response, we evaluated the cross-neutralization of convalescent plasmas and RBD-specific monoclonal antibodies (mAbs) against various spike protein-based pseudoviruses. Three of 24 SARS-CoV-2 pseudoviruses containing different mutations in spike protein, including D614G, A475V, and E484Q, consistently showed an altered sensitivity to neutralization by convalescent plasmas. A475V and E484Q mutants are highly resistant to neutralization by mAb B38 and 2-4, suggesting that some crucial mutations in spike protein might evolve SARS-CoV-2 variants capable of escaping humoral immune response.
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http://dx.doi.org/10.3389/fimmu.2021.693775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416052PMC
September 2021

Constrained-Spherical Deconvolution Tractography in the Evaluation of the Corticospinal Tract in Glioma Surgery.

Front Surg 2021 29;8:646465. Epub 2021 Jul 29.

Juha Hernesniemi International Neurosurgery Center, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, China.

Tractography has demonstrated utility for surgical resection in the setting of primary brain tumors involving eloquent white matter (WM) pathways. Twelve patients with glioma in or near eloquent motor areas were analyzed. The motor status was recorded before and after surgery. Two different tractography approaches were used to generate the motor corticospinal tract (CST): Constrained spherical deconvolution probabilistic tractography (CSD-Prob) and single tensor deterministic tractography (Tens-DET). To define the degree of disruption of the CST after surgical resection of the tumor, we calculated the percentage of the CST affected by surgical resection, which was then correlated with the postoperative motor status. Moreover, the fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) of the CST generated by the CSD-Prob and the Tens-DET was measured and compared between the ipsilesional and contralesional side. The CST was identified in all patients and its trajectory was displaced by the tumor. Only the CSD-Prob approach showed the CST with the characteristic fan-like projections from the precentral gyrus to the brainstem. Disruption of the CST was identified in 6/6 with postoperative motor deficit by CSD-Prob approach and in 5/6 in the Tens-DET. The degree of disruption was significantly associated with the motor deficit with the CSD-Prob approach ( = -0.88, = 0.021). However, with the Tens-DET approach the CST disruption did not show significant association with the motor function ( = -0.27, = 0.6). There was a significant decrease in FA ( = 0.006) and a significant increase in MD ( = 0.0004) and RD ( = 0.005) on the ipsilesional CST compared with the contralesional CST only with the CSD-Prob approach. CSD-Prob accurately represented the known anatomy of the CST and provided a meaningful estimate of microstructural changes of the CST affected by the tumor and its macrostructural damage after surgery. Newer surgical planning stations should include advanced models and algorithms of tractography in order to obtain more meaningful reconstructions of the WM pathways during glioma surgery.
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http://dx.doi.org/10.3389/fsurg.2021.646465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358074PMC
July 2021

Pulmonary function of healthy Korean children from three independent birth cohorts: Validation of the Global Lung Function Initiative 2012 equation.

Pediatr Pulmonol 2021 Oct 25;56(10):3310-3320. Epub 2021 Aug 25.

Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark.

Background And Objective: Global Lung Function Initiative (GLI) 2012 equations were developed to resolve the age-related disparity in interpreting spirometry results. Local validation of the equation is needed, especially in Northeast Asian children. This study evaluated the GLI equation in Korean children.

Methods: Spirometry indices (FEV1, FVC, FEV1/FVC, and FEF25%-75%) and clinical information were gathered from three population-based birth cohorts. Predicted GLI reference values and z scores of spirometry results were calculated for 1239 healthy children. The mean, standard deviation of z scores were compared with the expected 0 and 1. Probabilities of falling below the lower limit of normal (LLN) (z score: -1.64) were compared with the expected value 5%. GLI z scores were assessed according to low (<-2), normal (≥-2 and ≤2), and high (>2) BMI z score groups.

Results: Mean z scores significantly differed from 0 for FEV1/FVC in males (mean [95% confidence interval]: 0.18 [0.08, 0.27]) and forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) in females (-0.23 [-0.31, -0.15] and -0.26 [-0.36, -0.16], respectively). The standard deviation was larger than 1 for all variables in males and FVC and FEV1/FVC in females. The probability of falling below the LLN was significantly larger than 5% for FEV1 (12.13% [9.64, 14.77]), FVC (15.86% [13.06, 18.81]), and forced expiratory flow at 25%-75% of forced vital capacity (FEF25%-75%) (7.31% [5.29, 9.49]) in males and FVC (11.91% [9.40, 14.60]) in females. FEV1 and FVC z scores increased across low to high body mass index (BMI) groups, and FEV1/FVC decreased from low to high BMI groups.

Conclusion: GLI equations marginally differ from real-world values, which should be considered by pulmonologists in practice or research.
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http://dx.doi.org/10.1002/ppul.25622DOI Listing
October 2021

Centered Multi-Task Generative Adversarial Network for Small Object Detection.

Sensors (Basel) 2021 Jul 31;21(15). Epub 2021 Jul 31.

School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Despite the breakthroughs in accuracy and efficiency of object detection using deep neural networks, the performance of small object detection is far from satisfactory. Gaze estimation has developed significantly due to the development of visual sensors. Combining object detection with gaze estimation can significantly improve the performance of small object detection. This paper presents a centered multi-task generative adversarial network (CMTGAN), which combines small object detection and gaze estimation. To achieve this, we propose a generative adversarial network (GAN) capable of image super-resolution and two-stage small object detection. We exploit a generator in CMTGAN for image super-resolution and a discriminator for object detection. We introduce an artificial texture loss into the generator to retain the original feature of small objects. We also use a centered mask in the generator to make the network focus on the central part of images where small objects are more likely to appear in our method. We propose a discriminator with detection loss for two-stage small object detection, which can be adapted to other GANs for object detection. Compared with existing interpolation methods, the super-resolution images generated by CMTGAN are more explicit and contain more information. Experiments show that our method exhibits a better detection performance than mainstream methods.
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http://dx.doi.org/10.3390/s21155194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347084PMC
July 2021

The first reported outbreak of an undetermined species of human infection with spotted fever group Rickettsia in Lu'an, China.

Acta Trop 2021 Nov 3;223:106072. Epub 2021 Aug 3.

Lu'an Municipal Center for Disease Control and Prevention, Lu'an, China. Electronic address:

Objective: On May 2, 2017, an outbreak of unexplained fever with rashes was reported in Lu'an, China. In this study, we aimed to identify the possible pathogens, epidemiological characteristics, and risk factors of this outbreak.

Methods: We conducted descriptive field epidemiological studies. Blood samples were tested using an indirect immunofluorescence assay for Rickettsia rickettsii antibody, and nested polymerase chain reaction and gene sequencing assays were performed.

Results: We recruited 39 cases who had symptomatic onset from April 20 to June 8, 2017. Among these, 9 were suspected cases, 18 were probable cases, and 12 were confirmed cases. No one died. The main clinical manifestations were fever (100%), rash (100%), fatigue (97.3%), myalgia (83.8%), and anorexia (83.8%). None of the patients died. Thirty-seven patients who were treated with antibiotics during hospitalization showed significant improvement. The cases were distributed across 14 townships in 2 counties. The median age was 59 (43.0-81.0) years, of which 93.3% had a history of tea picking (28/30), and 77.3% (17/22) had a history of tick bites. The mean incubation period was 5.0 days (2.0-13.0 days). Serum IgG titers were higher in convalescent patients than in the general population (p = 0.016). Phylogenetic analysis revealed that the ompA sequences of Rickettsia sp. Lu'an-2018 had an 86.8%-99.0% sequence identity with the 23 strains of Rickettsia found worldwide.

Conclusions: This was the first reported outbreak of an undetermined species of a human infection with the spotted fever group of Rickettsia in China, which might be caused by ticks biting local residents when picking tea.
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http://dx.doi.org/10.1016/j.actatropica.2021.106072DOI Listing
November 2021

Diagnostic accuracy of coronary computed tomography angiography-derived fractional flow reserve.

Biomed Eng Online 2021 Aug 4;20(1):77. Epub 2021 Aug 4.

Department of Cardiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, China.

Background: Fractional flow reserve (FFR) is a widely used gold standard to evaluate ischemia-causing lesions. A new method of non-invasive approach, termed as AccuFFRct, for calculating FFR based on coronary computed tomography angiography (CCTA) and computational fluid dynamics (CFD) has been proposed. However, its diagnostic accuracy has not been validated.

Objectives: This study sought to present a novel approach for non-invasive computation of FFR and evaluate its diagnostic performance in patients with coronary stenosis.

Methods: A total of 54 consecutive patients with 78 vessels from a single center who underwent CCTA and invasive FFR measurement were retrospectively analyzed. The CT-derived FFR values were computed using a novel CFD-based model (AccuFFRct, ArteryFlow Technology Co., Ltd., Hangzhou, China). Diagnostic performance of AccuFFRct and CCTA in detecting hemodynamically significant coronary artery disease (CAD) was evaluated using the invasive FFR as a reference standard.

Results: Diagnostic accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for AccuFFRct in detecting FFR ≤ 0.8 on per-patient basis were 90.7, 89.5, 91.4, 85.0 and 94.1%, respectively, while those of CCTA were 38.9, 100.0, 5.71, 36.5 and 100.0%, respectively. The correlation between AccuFFRct and FFR was good (r = 0.76 and r = 0.65 on per-patient and per-vessel basis, respectively, both p < 0.0001). Area under the curve (AUC) values of AccuFFRct for identifying ischemia per-patient and per-vessel basis were 0.945 and 0.925, respectively. There was much higher accuracy, specificity and AUC for AccuFFRct compared with CCTA.

Conclusions: AccuFFRct computed from CCTA images alone demonstrated high diagnostic performance for detecting lesion-specific ischemia, it showed superior diagnostic power than CCTA and eliminated the risk of invasive tests, which could be an accurate and time-efficient computational tool for diagnosing ischemia and assisting clinical decision-making.
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http://dx.doi.org/10.1186/s12938-021-00914-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340407PMC
August 2021

Corrigendum: Effective velocities of polaron spin states in monolayer transition metal dichalcogenides (2021235303).

J Phys Condens Matter 2021 Jul 30;33(40). Epub 2021 Jul 30.

Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, School of Science, Department of Physics, Tianjin University, Tianjin 300354, People's Republic of China.

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http://dx.doi.org/10.1088/1361-648X/ac1536DOI Listing
July 2021

[Role of Stem Cells and Their Biomimetic Matrix Microenvironment in Regenerative Repair of Articular Cartilage: A Review].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Jul;52(4):548-554

National Engineering Research Center for Biomedical Materials, Sichuan University, Chengdu 610064, China.

It is difficult for the articular cartilage to self-heal any damage it may incur due to its lack of nerves and blood vessels. Development in stem cell technology provides new prospects for articular cartilage regeneration. Currently, stem cells from different sources and their diverse applications have demonstrated different degrees of therapeutic effect and potential in articular cartilage repair. However, stem cells are highly sensitive to their microenvironment. Therefore, more and more researchers are focusing their attention on regulating stem cells and thus accelerating cartilage regeneration through the biomimetic microenvironment constructed by biologically functional scaffolds. We reviewed in this paper the sources of the stem cells used for cartilage repair, the application method of these stem cells, as well as the therapeutic effect, mechanism and limitations in the application of stem cells synergizing with the biomimetic microenvironment in promoting articular cartilage repair and regeneration. We hoped to provide suggestions for practical clinical research in the design and improvement of biofunctional cartilage repair scaffolds that synergize with stem cells.
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http://dx.doi.org/10.12182/20210760301DOI Listing
July 2021

Triclabendazole Induces Pyroptosis by Activating Caspase-3 to Cleave GSDME in Breast Cancer Cells.

Front Pharmacol 2021 8;12:670081. Epub 2021 Jul 8.

Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC/VUmc Location and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universitetit Amsterdam, Amsterdam Movement Science, Amsterdam, Netherlands.

Pyroptosis is a form of programmed cell death, in which gasdermin E (GSDME) plays an important role in cancer cells, which can be induced by activated caspase-3 on apoptotic stimulation. Triclabendazole is a new type of imidazole in fluke resistance and has been approved by the FDA for the treatment of fascioliasis and its functions partially acting through apoptosis-related mechanisms. However, it remains unclear whether triclabendazole has obvious anti-cancer effects on breast cancer cells. In this study, to test the function of triclabendazole on breast cancer, we treated breast cancer cells with triclabendazole and found that triclabendazole induced lytic cell death in MCF-7 and MDA-MB-231, and the dying cells became swollen with evident large bubbles, a typical sign of pyroptosis. Triclabendazole activates apoptosis by regulating the apoptoic protein levels including Bax, Bcl-2, and enhanced cleavage of caspase-8/9/3/7 and PARP. In addition, enhanced cleavage of GSDME was also observed, which indicates the secondary necrosis/pyroptosis is further induced by active caspase-3. Consistent with this, triclabendazole-induced GSDME-N-terminal fragment cleavage and pyroptosis were reduced by caspase-3-specific inhibitor (Ac-DEVD-CHO) treatment. Moreover, triclabendazole induced reactive oxygen species (ROS) elevation and increased JNK phosphorylation and lytic cell death, which could be rescued by the ROS scavenger (NAC), suggesting that triclabendazole-induced GSDME-dependent pyroptosis is related to the ROS/JNK/Bax-mitochondrial apoptotic pathway. Besides, we showed that triclabendazole significantly reduced the tumor volume by promoting the cleavage of caspase-3, PARP, and GSDME in the xenograft model. Altogether, our results revealed that triclabendazole induces GSDME-dependent pyroptosis by caspase-3 activation at least partly through augmenting the ROS/JNK/Bax-mitochondrial apoptotic pathway, providing insights into this on-the-market drug in its potential new application in cancer treatment.
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http://dx.doi.org/10.3389/fphar.2021.670081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297466PMC
July 2021

LINC02381 contributes to cell proliferation and hinders cell apoptosis in glioma by transcriptionally enhancing CBX5.

Brain Res Bull 2021 Nov 15;176:121-129. Epub 2021 Jul 15.

Department of Neurosurgery, Henan Provincial People's Hospital, No.7 Weiwu Road, Jinshui District, Zhengzhou, 450003, Henan, China. Electronic address:

Glioma, featured with high incidence and low survival rate, is the most common type of primary brain tumor, severely affecting human life worldwide. LINC02381 is an interesting lncRNA functioning as oncogenic lncRNA in some cancers but as tumor-suppressor in others, but no report demonstrates its association with and function in glioma. Intriguingly, we found in a bioinformatics website LncRNADisease that LINC02381 was closely related to malignant glioma, so this study aimed to figure out the expression and function of LINC02381 in glioma. By RT-qPCR, we confirmed LINC02381 upregulation in glioma cells. Functional experiments demonstrated that LINC02381 knockdown repressed glioma cell proliferation and induced apoptosis. Boinformatics tools and RT-qPCR revealed the positive correlation between LINC02381 and CBX5 in glioma cells. More importantly, we confirmed that LINC02381 could interact and work synergistically with CEBPβ to bind to CBX5 promoter and activate CBX5 transcriptionally. Additionally, rescue experiments indicated that CBX5 up-regulation reversed the decline in cell proliferation and the augment in cell apoptosis caused by LINC02381 knockdown. To conclude, LINC02381 could facilitate CBX5 transcription via interaction with CEBPβ, thus exerting its oncogenic role in glioma cells, which could contribute to better understanding of glioma.
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http://dx.doi.org/10.1016/j.brainresbull.2021.07.009DOI Listing
November 2021

Quantifying the effects of co-composting organic biomass mixtures with inorganic amendments to obtain value-added bio-products.

PLoS One 2021 14;16(7):e0253714. Epub 2021 Jul 14.

Department of Agricultural Engineering, Bahauddin Zakariya University, Multan, Pakistan.

Co-digestion of organic biomass mixed with inorganic amendments could have an impact on composting dynamics. Various studies highlighted fertilizers' role as an additive to lesser the nitrogen loss, while some studies focused on the addition of fertilizers to enhance the efficiency. The changes in carbon, nitrogen components, and humic substances during the organic-inorganic co-compost process were seldom studied. Clarifying these changes might help improve the production process and compost nutrients contents. Thus, this study's purpose is to investigate the effects of inorganic amendments on compost characteristics, compost temperature, biochemical methane production (BMP), and nutritional contents. The inorganic phosphorous (P), sulfur (S), and sulfur solubilizing agent (SSA) were added to Farmyard manure (FYM) mixed with biodegradable waste (BW), including wheat straw, corn stalks, and green lawn waste. The P and S amended treatments were carried out into two sets, with and without SSA. The mixed feedstocks were added in the insulated RBC composting pit (15 x 15 x 10 feet). The compost material's moisture content was maintained 50-65% during the entire composting process for optimum waste digestion i.e., the moisture content (MC) of FYM was 82.7% and for BW ranged 8.8-10.2%, while the C/N ratio was found 10.5 for FYM, 74.5 for wheat straw, 83.5 for corn stalks, and 84.8 for lawn waste. At the condition of compost maturity, the inorganic amendments have no significant effect on composted material's moisture content. The maximum organic matter of 69.7% and C/N ratio of 44.6 was measured in T1. On the 6th day of composting, the temperature reached to thermophilic range (>45 oC) in all the treatments due to aeration of compost increased microbial activities and waste decomposition rate and decreased gradually to mesophilic range (35-45 oC) because the supply of high-energy compounds becomes exhausted. The highest temperature was reached in T4 (58 oC) and lowest in CT (47 oC). The significantly maximum methane of 8.95 m3 and biogas burning was 818 minutes in CT, followed by T1 and T4. The results of this study revealed that P enriched compost is a feasible and sustainable way to overcome P deficiency in the soil as well as in plants and best way to use low-grade P and organic waste material.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253714PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279329PMC
July 2021

Stimuli-responsive biphenyl-tripeptide supramolecular hydrogels as biomimetic extracellular matrix scaffolds for cartilage tissue engineering.

Acta Biomater 2021 09 8;131:128-137. Epub 2021 Jul 8.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan, 610064, PR China.

Supramolecular hydrogel composed of aromatic short peptide gelator was an attractive biomaterial owing to its simple and convenient synthetic route, nano-fibrillar microstructure resembling natural collagen fibers and intelligent response to external stimulus. Herein, stimuli-responsive biphenyl-tripeptide supramolecular hydrogels was prepared to simulate extracellular matrix scaffolds by temperature switch, ion induction and pH switch. The amino acid arrangement substantially affected gelation behavior, only BPAA-βAFF and BPAA-FFβA could form nanostructured supramolecular hydrogels with 8-10 nm nanotubes or nanofibers by potential intermolecular hydrogen bond interactions and π-π stacking. The minimum gelation concentration (MGC) and maximum storage modulus were 0.4 mM (0.023 wt%) and around 8.2 KPa. The two supramolecular hydrogels could support adhesion and proliferation of L929 cells. Moreover, the BPAA-βAFF hydrogel promoted proliferation and ECM secretion of chondrocytes in vitro, and facilitate the phenotype maintenance of hyaline cartilage. All the results demonstrated that BPAA-βAFF hydrogel hold great potential application prospects in cartilage tissue engineering. STATEMENT OF SIGNIFICANCE: Diphenylalanine was served as a core segment conjugating with 4-biphenylacetic acid (BPAA) to produce biphenyl-tripeptide compounds with transforming amino sequence, and multiple external stimuli was applied to study the gelation properties of the aromatic short peptide gelators. "FF" brick (phenylalanine-phenylalanine) was crucial for formation of fibrous supramolecular hydrogels. Meanwhile, the sequence of amino acids arrangement also had an essential effect on the gelation behavior. Optimal BPAA-βAFF with ultra-low minimum gelation concentration (0.4 mM, about 0.023 wt%) and similar microstructure to extracellular matrix (ECM) of nature cartilage tissue could promote the proliferation and ECM secretion of chondrocytes in vitro, and facilitate the formation of hyaline cartilage.
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http://dx.doi.org/10.1016/j.actbio.2021.07.007DOI Listing
September 2021

Overexpression of hsa_circ_0008274 inhibited the progression of lung adenocarcinoma by regulating HMGA2 via sponging miR-578.

Thorac Cancer 2021 08 8;12(16):2258-2264. Epub 2021 Jul 8.

Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.

Background: Circular RNAs (circRNAs) had been identified as a non-coding RNA associated with many types of cancer in recent years. However, the involvement of hsa_circ_0008274 in lung adenocarcinoma (LUAD) has not been explored. The aim of our research is to explore the biological mechanism and function of hsa_circ_0008274 in LUAD.

Methods: The hsa_circ_0008274, miR-578, and high mobility group AT-Hook 2 (HMGA2) mRNA expression levels were detected via qRT-PCR. Cell Counting Kit-8 (CCK-8) Transwell assay and wound healing assay were performed to measure the cell proliferation, invasion, and migration ability. Luciferase reporter and Western blotting experiments were performed to characterize the competing endogenous RNA (ceRNA) mechanism of hsa_circ_0008274.

Results: Our findings determined that the expression of hsa_circ_0008274 in LUAD was significantly decreased. Cell experiments showed that overexpressed hsa_circ_0008274 could reduce the proliferation and invasion ability of LUAD cells. Moreover, miRNA-578 could identify as a miRNA sponge of hsa_circ_0008274. Overexpressed hsa_circ_0008274 reduced the proliferation and invasion of LUAD cells caused by miR-578 mimics. Increasing the expression of miR-578 can aggravate the proliferation and invasion of LUAD cells and block the inhibition of proliferation and invasion of LUAD cells mediated by overexpressed hsa_circ_0008274. Subsequent data indicate that HMGA2 of the tumor-promoting gene is the target gene of miR-578. The upregulation of HMGA2 partially reversed the tumor inhibitory effect of LUAD cells induced by overexpressed hsa_circ_0008274 or miR-578 mimics.

Conclusions: In summary, our data show that the overexpression of hsa_circ_0008274 repressed the proliferation and invasion of LUAD through downregulating miR-578 and activating HMGA2.
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http://dx.doi.org/10.1111/1759-7714.14059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364990PMC
August 2021

Emergence of a young case infected with avian influenza A (H5N6) in Anhui Province, East China during the COVID-19 pandemic.

J Med Virol 2021 10 14;93(10):5998-6007. Epub 2021 Jul 14.

Microbiological Laboratory, Anhui Provincial Center for Disease Control and Prevention, Hefei, Anhui, China.

In the context of the coronavirus disease 2019 pandemic, we investigated the epidemiological and clinical characteristics of a young patient infected by avian influenza A (H5N6) virus in Anhui Province, East China, and analyzed genomic features of the pathogen in 2020. Through the cross-sectional investigation of external environment monitoring (December 29-31, 2020), 1909 samples were collected from Fuyang City. It was found that the positive rate of H5N6 was higher than other areas obviously in Tianma poultry market, where the case appeared. In addition, dual coinfections were detected with a 0.057% polymerase chain reaction positive rate the surveillance years. The virus was the clade 2.3.4.4, which was most likely formed by genetic reassortment between H5N6 and H9N2 viruses. This study found that the evolution rates of the hemagglutinin and neuraminidase genes of the virus were higher than those of common seasonal influenza viruses. The virus was still highly pathogenic to poultry and had a preference for avian receptor binding.
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http://dx.doi.org/10.1002/jmv.27179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426836PMC
October 2021

Adsorption of tetracycline by seed gum and seed gum/Carboxymethyl chitosan aerogel.

Environ Technol 2021 Jul 8:1-12. Epub 2021 Jul 8.

State Key Laboratory of Bio-fibers and Eco-textiles, College of Mechanical and Electrical Engineering, Qingdao University, Qingdao, People's Republic of China.

In this study, novel aerogels of seed gum (NPG) and seed gum/Carboxymethyl chitosan (NPG/CMC) were prepared by freeze-drying method for removing tetracycline (TC) from water. Scanning electron microscope (SEM), X-ray diffraction (XRD),Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and Brunauer-Emmett-Teller (BET) were used to characterize structure and morphology of NPG and NPG/CMC aerogels. The average pore diameter of NPG and NPG/CMC were 3.04 and 1.2 nm, the specific surface areas were 2.67 and 0.73 m/g, respectively. The maximum adsorption capacity of NPG and NPG/CMC aerogels for TC based on Langmuir isotherm was 266.7 and 332.23 mg/g respectively. Through thermodynamic and kinetic studies, it was found that the adsorption processes of the two adsorbents were spontaneous and followed the pseudo-second-order kinetic model. And the process of NPG adsorption of TC was endothermic, while NPG/CMC was exothermic.
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http://dx.doi.org/10.1080/09593330.2021.1946166DOI Listing
July 2021

Corrigendum to "Reduction-sensitive polymeric micelles as amplifying oxidative stress vehicles for enhanced antitumor therapy" [Colloids Surf. B: Biointerfaces 203 (2021) 111733].

Colloids Surf B Biointerfaces 2021 Oct 17;206:111924. Epub 2021 Jun 17.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China.

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http://dx.doi.org/10.1016/j.colsurfb.2021.111924DOI Listing
October 2021

Rapid detection of cellulose and hemicellulose contents of corn stover based on near-infrared spectroscopy combined with chemometrics.

Appl Opt 2021 May;60(15):4282-4290

The feasibility of near-infrared spectroscopy (NIRS) combined with chemometrics for the rapid detection of the cellulose and hemicellulose contents in corn stover is discussed. Competitive adaptive reweighted sampling (CARS) and genetic simulated annealing algorithm (GSA) were combined (CARS-GSA) to select the characteristic wavelengths of cellulose and hemicellulose and to reduce the dimensionality and multicollinearity of the NIRS data. The whole spectra contained 1845 wavelength variables. After CARS-GSA optimization, the number of characteristic wavelengths of cellulose (hemicellulose) was reduced to 152 (260), accounting for 8.24% (14.09%) of all wavelengths. The coefficients of determination of the regression models for predicting the cellulose and hemicellulose contents were 0.968 and 0.996, the root mean square errors of prediction (RMSEPs) were 0.683 and 0.648, and the residual predictive deviations (RPDs) were 5.213 and 16.499, respectively. The RMSEP of the cellulose and hemicellulose regression models was 0.152 and 0.190 lower for CARS-GSA than for the full-spectrum, and the RPD was increased by 0.949 and 3.47, respectively. The results showed that the CARS-GSA model substantially reduced the number of characteristic wavelengths and significantly improved the predictive ability of the regression model.
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http://dx.doi.org/10.1364/AO.418226DOI Listing
May 2021

Bioinspired polysaccharide hybrid hydrogel promoted recruitment and chondrogenic differentiation of bone marrow mesenchymal stem cells.

Carbohydr Polym 2021 Sep 19;267:118224. Epub 2021 May 19.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan 610064, PR China; College of Biomedical Engineering, Sichuan University, 29 Wangjiang Road, Chengdu 610064, PR China.

Cartilage regeneration by biomimetic cartilage matrix with synchronously recruited stem cells was one of ideal strategies. Inspired by catechol for proteins adhesion, dopamine modified polysaccharide hybrid hydrogel (HD-C) was prepared by integrating collagen I (Col I) and hyaluronic acid derivatives (HA-DN) with sulfhydryl modified polysaccharide hybrid hydrogel (HS-C) as control. Because of double-crosslinking architecture, HD-C hydrogel was endowed with a more compact pore structure, higher mechanical properties and water retention ability in comparison with those of HS-C hydrogel. Meanwhile, it significantly promoted the proliferation and spread of rabbit bone marrow stem cells (rBMSCs), and accelerated cartilaginous matrix secretion. RT-PCR results also verified higher related gene expression of chondrogenesis (Sox 9, Agg and Col II). Moreover, HD-C hydrogel could enhance the enrichment and migration of rBMSCs in vitro by potential functional protein adsorption mechanisms, and this phenomenon was further confirmed by more rBMSCs migration in short-term joint implantation experiments in vivo.
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http://dx.doi.org/10.1016/j.carbpol.2021.118224DOI Listing
September 2021

Liberation of insoluble-bound phenolics from lentil hull matrices as affected by Rhizopus oryzae fermentation: Alteration in phenolic profiles and their inhibitory capacities against low-density lipoprotein (LDL) and DNA oxidation.

Food Chem 2021 Nov 3;363:130275. Epub 2021 Jun 3.

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada. Electronic address:

Fermentation is an effective non-thermal food processing operation used for enhancing the nutritional and functional properties of food. HPLC-ESI-MS/MS analysis and inhibitory capacity of the soluble- and insoluble-bound phenolics in lentil hulls in retarding the oxidation of LDL and DNA strand scission were determined following fermentation. In HPLC-ESI-MS/MS analysis, most insoluble-bound phenolics in lentil hulls were significantly decreased, indicating their liberation from the cell wall matrix upon fermentation. However, the released insoluble-bound phenolics did not show an efficient conversion into the bioavailable soluble phenolics as reflected in the inhibitory capacity against oxidation of LDL and DNA strands. The low efficiency in bioconversion from insoluble-bound to soluble phenolics might be due to the loss of the released bound phenolics during the fermentation process. Following the alterations of individual insoluble-bound phenolics in legumes upon fermentation in this work may fill the existing gap in the related areas.
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http://dx.doi.org/10.1016/j.foodchem.2021.130275DOI Listing
November 2021

LncRNA Bmp1 promotes the healing of intestinal mucosal lesions via the miR-128-3p/PHF6/PI3K/AKT pathway.

Cell Death Dis 2021 06 9;12(6):595. Epub 2021 Jun 9.

Department of Burn Surgery, the Affiliated Huaihai Hospital of Xuzhou Medical University, Xuzhou, 221004, Jiangsu Province, China.

Intestinal mucosal injuries are directly or indirectly related to many common acute and chronic diseases. Long non-coding RNAs (lncRNAs) are expressed in many diseases, including intestinal mucosal injury. However, the relationship between lncRNAs and intestinal mucosal injury has not been determined. Here, we investigated the functions and mechanisms of action of lncRNA Bmp1 on damaged intestinal mucosa. We found that Bmp1 was increased in damaged intestinal mucosal tissue and Bmp1 overexpression was able to alleviate intestinal mucosal injury. Bmp1 overexpression was found to influence cell proliferation, colony formation, and migration in IEC-6 or HIEC-6 cells. Moreover, miR-128-3p was downregulated after Bmp1 overexpression, and upregulation of miR-128-3p reversed the effects of Bmp1 overexpression in IEC-6 cells. Phf6 was observed to be a target of miR-128-3p. Furthermore, PHF6 overexpression affected IEC-6 cells by activating PI3K/AKT signaling which was mediated by the miR-128-3p/PHF6 axis. In conclusion, Bmp1 was found to promote the expression of PHF6 through the sponge miR-128-3p, activating the PI3K/AKT signaling pathway to promote cell migration and proliferation.
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http://dx.doi.org/10.1038/s41419-021-03879-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190101PMC
June 2021

Psychometric Assessment of the Chinese Version of the Indian Vision Functioning Questionnaire Based on the Method of Successive Dichotomizations.

Transl Vis Sci Technol 2021 06;10(7)

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: The purpose of this study was to assess whether a Chinese translated version of the 33-item Indian Vision Function Questionnaire (IND-VFQ-33) forms a valid measurement scale and to evaluate its psychometric properties based on the method of successive dichotomizations (MSD).

Methods: The English version of the IND-VFQ-33 was translated, back translated, and cross-culturally adapted for use in China. It was interviewer administered to patients with cataracts. MSD, a polytomous Rasch model that estimates ordered thresholds, was used to assess and optimize psychometric properties of the overall scale and three subscales separately.

Results: One hundred and seventy-nine patients provided complete responses. After the removal of 2 misfitting items, a revised 31-item overall scale demonstrated adequate precision (person reliability [PR] = 0.92) and no misfitting items. The general functioning subscale fit the MSD model well after removing two misfitting items. The psychosocial impact subscale and the visual symptoms subscale were not considered further due to poor measurement precision. After addressing psychometric deficiencies, a 31-item overall scale (IND-VFQ-31-CN) and a 19-item general functioning subscale (IND-VFQ-GF-19-CN) were developed.

Conclusions: The original IND-VFQ-33 required re-engineering to form valid measures for use in China. The revised overall scale and general functioning subscale demonstrated adequate MSD based psychometric properties.

Translational Relevance: The revised IND-VFQ-33 is a valid patient-reported outcome assessment for Chinese patients with cataract based on MSD analysis.
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http://dx.doi.org/10.1167/tvst.10.7.8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196417PMC
June 2021

Pathogenic mechanism, detection methods and clinical significance of group B .

Future Microbiol 2021 Jun 8;16:671-685. Epub 2021 Jun 8.

Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, Shandong, 264200, PR China.

Group B (GBS) is the main pathogen of perinatal infection. It can lead to adverse pregnancy, maternal infection, premature delivery, abortion, stillbirth and a series of adverse maternal and infant outcomes such as neonatal sepsis, meningitis or pneumonia during delivery. In order to reduce the infection of perinatal pregnant and the adverse pregnancy outcome, more attention should be paid in the clinical practice, screening efforts, universal detection of GBS infection for pregnant women and preventive treatment for the possible mother infant infection. In this study, the biological characteristics, immunophenotype, major pathogenic mechanism, laboratory test methods and clinical significance of GBS are summarized.
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http://dx.doi.org/10.2217/fmb-2020-0189DOI Listing
June 2021
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