Publications by authors named "Yong Peng"

654 Publications

Fatigue fractures after the COVID-19 quarantine.

Phys Sportsmed 2021 May 7. Epub 2021 May 7.

Department of Radiology, General Hospital of Ningxia Medical University Yinchuan, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00913847.2021.1927232DOI Listing
May 2021

observation of the crystal structure transition of Pt-Sn intermetallic nanoparticles during deactivation and regeneration.

Chem Commun (Camb) 2021 May 6. Epub 2021 May 6.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

The mechanism of the deactivation and regeneration of PtSn intermetallic compound nanoparticle (iNP) catalysts was studied by in situ TEM investigation. Our study reveals the reversible dynamic structural transition of the iNPs during deactivation and regeneration, which provides a direct correlation between the atomic structure and the catalytic activity of the iNPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc01181bDOI Listing
May 2021

Risky behaviors, psychological failures and kinematics in vehicle-to-powered two-wheeler accidents: Results from in-depth Chinese crash data.

Accid Anal Prev 2021 Apr 28;156:106150. Epub 2021 Apr 28.

Key Laboratory of Traffic Safety on Track of Ministry of Education, School of Traffic & Transportation Engineering, Central South University, Changsha, 410075, China.

As the use of powered two-wheeler (PTW) becomes increasingly prevalent, PTW accidents are emerging as a major threat to the people's life and property in China. Understanding the risky behaviors, psychological failures and kinematics in vehicle-to-PTW accidents is an important first step in addressing this issue. Here 69 vehicle-to-PTW accidents captured on video from the Traffic Accident Investigation and Research in China (TAIRC) database are selected and reconstructed. All accidents are categorized into different crash scenarios using a harmonized method. Accident causations are identified from the perspectives of praxeology and psychology. Kinematics characteristics, such as impact speed and relative position, are also analyzed. The results show the crossing accident bundle is the most frequent followed by rear, oncoming and run-up accident bundles, with proportions of 43.48 %, 27.54 %, 11.59 % and 17.39 % respectively. Accident causations of different crash scenarios have great differences whether in accident responsibilities or in psychological failures. For instance, the PTW riders who violate the traffic regulations need to be mainly responsible for most crossing accidents, whereas most rear accidents are blamed on drivers who fail to properly check their mirrors when they turn, turn around or change lanes. From the perspective of psychology, the perception failures encountered by both drivers and riders are a typical causation in crossing accidents, while it is a contributing factor in rear accidents that a failure of prognosis from the rider combined with a failure of perception from the driver. Visual obstruction exists widely in crossing and oncoming accident bundles. The impact speeds of vehicles and PTWs are often less than 40 km/h in all accident bundles. A wider sensing area (field of view = 90°, view detection range = 35 m) should be achieved to more effectively detect the conflict PTWs. These findings about vehicle-PTW accidents provide a stronger support for the development of prevention countermeasures and advanced driver assistance system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aap.2021.106150DOI Listing
April 2021

Effects of Concentration and Spin Speed on the Optical and Electrical Properties of Silver Nanowire Transparent Electrodes.

Materials (Basel) 2021 Apr 26;14(9). Epub 2021 Apr 26.

College of Materials Science and Technology, Nanjing University of Science and Technology, Nanjing 210014, China.

In this paper, silver nanowires (AgNWs) with a diameter of 40 nm and a length of 45 μm were dispersed into an ethanol solution to prepare AgNW solutions with concentrations of 1, 2, and 3 mg/mL, respectively. The AgNW solutions were then deposited on a glass substrate using spin-coating at 1000, 2000, and 3000 rpm for 45 s, respectively, to prepare transparent electrodes. The results showed that the distribution of AgNWs on the substrate increased in density with the increase in the AgNW solution concentration and the decrease in spin speed. The effect of concentration on the distribution of AgNWs was greater than that of the spin speed. The transmittance of each electrode was between 84.19% and 88.12% at 550 nm, the average sheet resistance was between 20.09 and 358.11 Ω/sq, the highest () was 104.42, and the lowest was 1.48%. The electrode prepared at 1000 rpm with a concentration of 2 mg/mL and that prepared at 3000 rpm with a concentration of 3 mg/mL were very similar in terms of the average sheet resistance, transmittance at 550 nm, , and ; thus, these two electrodes could be considered equivalent. The of the electrode was positively correlated with the spin speed at low concentration, but that relationship became inverse as the concentration rose. For the AgNWs used in this experiment with an aspect ratio of 1125, the concentration of the AgNW solution should reach at least 2 mg/mL to ensure that the of the electrode is greater than 35.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14092219DOI Listing
April 2021

Rapid and Low-Input Profiling of Histone Marks in Plants Using Nucleus CUT&Tag.

Front Plant Sci 2021 12;12:634679. Epub 2021 Apr 12.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

Characterizing genome-wide histone posttranscriptional modifications and transcriptional factor occupancy is crucial for deciphering their biological functions. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful method for genome-wide profiling of histone modifications and transcriptional factor-binding sites. However, the current ChIP-seq experimental procedure in plants requires significant material and several days for completion. CUT&Tag is an alternative method of ChIP-seq for low-sample and single-cell epigenomic profiling using protein A-Tn5 transposase fusion proteins (PAT). In this study, we developed a nucleus CUT&Tag (nCUT&Tag) protocol based on the live-cell CUT&Tag technology. Our results indicate that nCUT&Tag could be used for histone modifications profiling in both monocot rice and dicot rapeseed using crosslinked or fresh tissues. In addition, both active and repressive histone marks such as H3K4me3 and H3K9me2 can be identified using our nCUT&Tag. More importantly, all the steps in nCUT&Tag can be finished in only 1 day, and the assay can be performed with as little as 0.01 g of plant tissue as starting materials. Therefore, our results demonstrate that nCUT&Tag is an efficient alternative strategy for plant epigenomic studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.634679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072009PMC
April 2021

Non-coding RNAs in human cancer.

Authors:
Yong Peng

Semin Cancer Biol 2021 Apr 21. Epub 2021 Apr 21.

Laboratory of Molecular Oncology, Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.semcancer.2021.04.010DOI Listing
April 2021

Construction of the Prediction Model for Locally Advanced Rectal Cancer Following Neoadjuvant Chemoradiotherapy Based on Pretreatment Tumor-Infiltrating Macrophage-Associated Biomarkers.

Onco Targets Ther 2021 13;14:2599-2610. Epub 2021 Apr 13.

Department of Colorectal Surgery, Fujian Medical University Union Hospital, Fuzhou, People's Republic of China.

Purpose: To assess the value of macrophage-related biomarkers (CD163, CD68, MCSF, and CCL2) for predicting the response to neo-chemoradiotherapy (NCRT) and the prognosis of locally advanced rectal cancer (LARC).

Methods: We enrolled 191 patients who underwent neoadjuvant chemoradiotherapy and radical resection between 2011 and 2015. Tumor tissues were collected before NCRT with a colonoscope and post-surgery and were subjected to immunohistochemical analysis.

Results: The expression levels of macrophage-related biomarkers (CD163, CD68, MCSF, and CCL2) were lower in the pathological complete response (pCR) group when compared with the non-pCR group (all P<0.05). Based on X-tile plots, we divided the tumors in two groups and found that lower pre-NCRT/post-surgical CD163, CD68, MCSF, CCL2 scores correlated with improved DFS. Cox regression analysis demonstrated that pre-NCRT CD163 (HR=1.008, 95% CI 1.003-1.013, =0.003) and MCSF (HR=2.187, 95% CI 1.343-3.564, =0.002) scores were independent predictors of DFS. Based on Cox multivariate analysis, we constructed a risk score model with a powerful ability to predict pCR in LARC patients. Moreover, COX regression analysis was performed to explore the role of the risk score in LARC patients. The results demonstrated that tumor size (HR=1.291, =0.041), worse pathological TNM stage (HR=1.789, =0.005, and higher risk score (HR=1.084, <0.001) were significantly associated with impaired disease-free survival. Based on the above results, a nomogram and decision curve analysis were generated.

Conclusion: The expression levels of macrophage-related biomarkers CD163, CD68, MCSF, and CCL2 were associated with chemoradiotherapy resistance and prognosis in LARC patients following NCRT. A risk score model was constructed which could be used to predict LARC outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S297263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053511PMC
April 2021

Atomic Sulfur Filling Oxygen Vacancies Optimizes H Absorption and Boosts the Hydrogen Evolution Reaction in Alkaline Media.

Angew Chem Int Ed Engl 2021 Apr 11. Epub 2021 Apr 11.

State Key Laboratory of Applied Organic Chemistry, Frontiers Science Center for Rare Isotopes, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

The hydrogen evolution reaction (HER) usually has sluggish kinetics in alkaline solution due to the difficulty in forming binding protons. Herein we report an electrocatalyst in which sulfur atoms are doping in the oxygen vacancies (V ) of inverse spinel NiFe O (S-NiFe O ) to create active sites with enhanced electron transfer capability. This electrocatalyst has an ultralow overpotential of 61 mV at the current density of 10 mA cm and long-term stability of 60 h at 1.0 Acm in 1.0 M KOH media. In situ Raman spectroscopy revealed that S sites adsorb hydrogen adatom (H*) and in situ form S-H*, which favor the production of hydrogen and boosts HER in alkaline solution. DFT calculations further verified that S introduction lowered the energy barrier of H O dissociation. Both experimental and theoretical investigations confirmed S atoms are active sites of the S-NiFe O .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202104055DOI Listing
April 2021

A new Kmeans clustering model and its generalization achieved by joint spectral embedding and rotation.

PeerJ Comput Sci 2021 30;7:e450. Epub 2021 Mar 30.

School of Computer Science and Technology, Hangzhou Dianzi University, Hangzhou, Zhejiang, China.

The Kmeans clustering and spectral clustering are two popular clustering methods for grouping similar data points together according to their similarities. However, the performance of Kmeans clustering might be quite unstable due to the random initialization of the cluster centroids. Generally, spectral clustering methods employ a two-step strategy of spectral embedding and discretization postprocessing to obtain the cluster assignment, which easily lead to far deviation from true discrete solution during the postprocessing process. In this paper, based on the connection between the Kmeans clustering and spectral clustering, we propose a new Kmeans formulation by joint spectral embedding and spectral rotation which is an effective postprocessing approach to perform the discretization, termed KMSR. Further, instead of directly using the dot-product data similarity measure, we make generalization on KMSR by incorporating more advanced data similarity measures and call this generalized model as KMSR-G. An efficient optimization method is derived to solve the KMSR (KMSR-G) model objective whose complexity and convergence are provided. We conduct experiments on extensive benchmark datasets to validate the performance of our proposed models and the experimental results demonstrate that our models perform better than the related methods in most cases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj-cs.450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022527PMC
March 2021

Clinical characteristics and in-hospital outcomes of patients receiving contemporary intensive cardiac care: retrospective study from a large centre in China.

J Geriatr Cardiol 2021 Feb;18(2):94-103

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.

Background: Few studies from developed countries have quantitatively characterized the clinical characteristics and outcomes of patients receiving contemporary intensive cardiac care. We sought to investigate these data in patients admitted to a Chinese intensive cardiac care unit (ICCU).

Methods: We conducted a retrospective study using data from 2,337 consecutive admissions to the ICCU at a large centre in China from June 2016 to May 2017. Data were captured after systematic inspection of individual medical records regarding current demographics, primary diagnosis, comorbidities, illnesses severity, and in-hospital outcomes.

Results: The mean age was 65.6 ± 14.2 years, and females accounted for 32.0% of patients. The Charlson Comorbidity Index and Oxford Acute Severity of Illness Score were 2.4 ± 1.8 and 22.5 ± 10.4, respectively. The top reason for admission was ST-segment elevation myocardial infarction (32.0%), and nonischaemic heart diseases accounted for 31.2% of all primary diagnoses. Noncardiovascular diseases were prevalent in the ICCU population, including chronic illnesses and acute noncardiovascular critical illnesses (ANCIs); in particular, 21.7% of patients were marked by acute respiratory failure (14.6%), acute kidney injury (13.7%), sepsis (4.2%), or gastrointestinal bleeding (3.3%). The median length of stay in the ICCU and hospital were 1.1 days [interquartile range (IQR): 0.8-2.6 days] and 6.3 days (IQR: 3.8-10.9 days), respectively. The overall incidence of in-hospital death or discharge against medical advice under extremely critical conditions was 7.6% ( = 177). Multivariate logistic regression analysis showed that the complexity of chronic illnesses and incident ANCIs were strong independent determinants for in-hospital outcomes.

Conclusions: Remarkable patient diversity and breadth of critical illnesses were observed in a Chinese ICCU population. Particularly, noncardiovascular diseases were prevalent and associated with adverse outcomes. Reformation of organization and staffing practices may be considered to adapt to the changed landscape.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11909/j.issn.1671-5411.2021.02.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940964PMC
February 2021

Syphilitic Aortitis Causing Severe Bilateral Coronary Ostial Stenosis.

JACC Cardiovasc Interv 2021 Apr 17;14(7):e65-e67. Epub 2021 Mar 17.

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, People's Republic of China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcin.2021.01.023DOI Listing
April 2021

Porous Graphitic Carbons Containing Nitrogen by Structuration of Chitosan with Pluronic P123.

ACS Appl Mater Interfaces 2021 Mar 11;13(11):13499-13507. Epub 2021 Mar 11.

Instituto Universitario de Tecnología Química, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Av. de los Naranjos s/n, 46022 Valencia, Spain.

Using Pluronic P123 as a structure-directing agent and chitosan as a carbon precursor, different porous carbons with remarkable morphologies such as orthohedra or spheres with diametrically opposite holes are obtained. These particles of micrometric size are constituted by the stacking of thin sheets (60 nm) that become increasingly bent in the opposite sense, concave in the upper and convex in the bottom hemispheres, as the chitosan proportion increases. TEM images, after dispersion of the particles by sonication, show that besides micrometric graphene sheets, the material is constituted by nanometric onion-like carbons. The morphology and structure of these porous carbons can be explained based on the ability of Pluronic P123 to undergo self-assembly in aqueous solution due to its amphoteric nature and the filmogenic properties of chitosan to coat Pluronic P123 nanoobjects undergoing structuration and becoming transformed into nitrogen-doped graphitic carbons. XPS analysis reveals the presence of nitrogen in their composition. These porous carbons exhibit a significant CO adsorption capacity of above 3 mmol g under 100 kPa at 273 K attributable to their large specific surface area, ultraporosity, and the presence of basic N sites. In addition, the presence of dopant elements in the graphitic carbons opening the gap is responsible for the photocatalytic activity for H generation in the presence of sacrificial electron donors, reaching a H production of 63 μmol g in 24 h.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c19463DOI Listing
March 2021

A Self-Healing Ionic Liquid-Based Ionically Cross-Linked Gel Polymer Electrolyte for Electrochromic Devices.

Polymers (Basel) 2021 Feb 27;13(5). Epub 2021 Feb 27.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.

An ionic liquid-based ionically cross-linked gel polymer electrolyte (GPE-ILs) was successfully synthesized using acrylic acid, 2-diethylaminoethyl methacrylate, methyl methacrylate, and ionic liquids. Electrochromic devices (ECDs) with an architecture of glass/FTO/WO/GPE-ILs/FTO/glass were fabricated by a laminating technology. The devices showed performances of large optical modulation of 49.9% at 650 nm, short switching times with the coloration time (tc) of 7 s and the bleaching time (tb) of 4 s, high coloration efficiency of 96.2 cm C, and cycling stability of 200 cycles. The GPE-ILs exhibits high ionic conductivity, superior thermal stability and good self-healing ability. GPE-ILs demonstrates an ionic conductivity of 3.19 × 10 S cm at 25 °C and the same ions migration behaviors with most widely used liquid electrolyte between -10 and 80 °C maintains more than 80% of its tensile strength after self-healing and received only 5% weight loss at 300 °C.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym13050742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957721PMC
February 2021

Investigation of the Ballistic Performance of GFRP Laminate under 150 m/s High-Velocity Impact: Simulation and Experiment.

Polymers (Basel) 2021 Feb 17;13(4). Epub 2021 Feb 17.

Hunan Industry Polytechnic, Changsha 410208, China.

The ballistic resistance of GFRP laminates subjected to high-velocity impact was studied. Based on the damage situation of GFRP laminate observed from the single-stage gas gun testing, the three-dimensional (3D) model combining strain rate effect and Hashin failure criterion was established, and the result presented good agreement between the simulation and experiment. Three factors, including layer angle, stacking sequence and proportion of different layer angles, were taken into consideration in the models. An orthogonal test method was used for the analysis, which can reduce the number of simulations effectively without sacrificing the accuracy of the result. The result indicated a correlation between the ballistic resistance and layouts of GFRP laminates, on which the stacking sequence contributed stronger influence. What was more, the laminate with layer angles 0°/90° and ±45° presented greater ballistic resistance than the other angle pairs, and adopting an equal proportion of different layer angles is helpful for GFRP laminates to resist impact as well.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym13040604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922781PMC
February 2021

Transcatheter and Surgical Aortic Valve Replacement in Patients With Previous Cardiac Surgery: A Meta-Analysis.

Front Cardiovasc Med 2020 10;7:612155. Epub 2021 Feb 10.

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.

Many patients who have aortic stenosis and are transcatheter aortic valve replacement (TAVR) candidates have underwent prior cardiac surgery (PCS). The aim of this study was to provide a robust summary comparison between patients with PCS who underwent TAVR vs. surgical aortic valve replacement (SAVR). We conducted a systematic review and meta-analysis of all published articles on PubMed/Medline, Ovid, EMBASE, and Scopus from 2002 to 2019. A total of 13 studies were finally included, yielding a total of 23,148 participants. There was no statistical difference with 30-day [OR: 1.02 (0.86-1.21)] or 1-year mortality [OR: 1.18 (0.86-1.61)] between the two groups. Subgroup analysis revealed that high-risk patients who underwent TAVR with the transapical approach were associated with increased risk of mortality [OR: 1.45 (1.00-2.11)]. However, those who underwent TAVR with endovascular approach had a comparable outcome with SAVR. Primary outcomes after endovascular TAVR were similar to those with SAVR and superior to transapical TAVR treatment group in patients with PCS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2020.612155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902485PMC
February 2021

Rationale and design of the OPTIMAL-REPERFUSION trial: A prospective randomized multi-center clinical trial comparing different fibrinolysis-transfer percutaneous coronary intervention strategies in acute ST-segment elevation myocardial infarction.

Clin Cardiol 2021 Apr 25;44(4):455-462. Epub 2021 Feb 25.

Department of Cardiology, West China Hospital of Sichuan University, Chengdu, China.

Primary percutaneous coronary intervention (PPCI), the preferred reperfusion strategy for all acute ST-segment elevation myocardial infarction (STEMI) patients, is not universally available in clinical practice. Pharmacoinvasive strategy has been proposed as a therapeutic option in patients with STEMI when timely PPCI is not feasible. However, pharmacoinvasive strategy has potential delay between clinical patency and complete myocardial perfusion. The optimal reperfusion strategy for STEMI patients with anticipated PPCI delay according to current practice is uncertain. OPTIMAL-REPERFUSION is an investigator-initiated, prospective, multicenter, randomized, open-label, superiority trial with blinded evaluation of outcomes. A total of 632 STEMI patients presenting within 6 hours after symptom onset and with an expected time of first medical contact to percutaneous coronary intervention (PCI) ≥120 minute will be randomized to a reduced-dose facilitated PCI strategy (reduced-dose fibrinolysis combined with simultaneous transfer for immediate invasive therapy with a time interval between fibrinolysis to PCI < 3 hours) or to standard pharmacoinvasive treatment. The primary endpoint is the composite of death, reinfarction, refractory ischemia, congestive heart failure, or cardiogenic shock at 30-days. Enrollment of the first patient is planned in March 2021. The recruitment is anticipated to last for 12 to 18 months and to complete in September 2023 with 1 year follow-up. The OPTIMAL-REPERFUSION trial will help determine whether reduced-dose facilitated PCI strategy improves clinical outcomes in patients with STEMI and anticipated PPCI delay. This study is registered with the ClinicalTrials.gov (NCT04752345).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/clc.23582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027583PMC
April 2021

Genomic evolution and diverse models of systemic metastases in colorectal cancer.

Gut 2021 Feb 25. Epub 2021 Feb 25.

Department of Gastrointestinal Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Objective: The systemic spread of colorectal cancer (CRC) is dominated by the portal system and exhibits diverse patterns of metastasis without systematical genomic investigation. Here, we evaluated the genomic evolution of CRC with multiorgan metastases using multiregion sequencing.

Design: Whole-exome sequencing was performed on multiple regions (n=74) of matched primary tumour, adjacent non-cancerous mucosa, liver metastasis and lung metastasis from six patients with CRC. Phylogenetic reconstruction and evolutionary analyses were used to investigate the metastatic seeding pattern and clonal origin. Recurrent driver gene mutations were analysed across patients and validated in two independent cohorts. Metastatic assays were performed to examine the effect of the novel driver gene on the malignant behaviour of CRC cells.

Results: Based on the migration patterns and clonal origins, three models were revealed (sequential, branch-off and diaspora), which not only supported the anatomic assumption that CRC cells spread to lung after clonally expanding in the liver, but also illustrated the direct seeding of extrahepatic metastases from primary tumours independently. Unlike other cancer types, polyphyletic seeding occurs in CRC, which may result in late metastases with intermetastatic driver gene heterogeneity. In cases with rapid dissemination, we found recurrent trunk loss-of-function mutations in , which is enriched in metastatic CRC and associated with poor overall survival. CRISPR/Cas9-mediated knockout of enhances the metastatic potential of CRC cells.

Conclusion: Our results provide genomic evidence for metastatic evolution and indicate that biopsy/sequencing of metastases may be considered for patients with CRC with multiorgan or late postoperative metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/gutjnl-2020-323703DOI Listing
February 2021

Effect of strain engineering on superlubricity in a double-walled carbon nanotube.

Phys Chem Chem Phys 2021 Mar;23(8):4988-5000

Key Laboratory of Traffic Safety on Track (Central South University), Ministry of Education, School of Traffic & Transportation Engineering, Central South University, Changsha, 410075, China. and Joint International Research Laboratory of Key Technology for Rail Traffic Safety, Central South University, Changsha, 410075, China.

Double-walled carbon nanotubes (DWCNTs) have received a great deal of attention due to their great potential in the field of superlubricity. However, this superlubricity is susceptible to failure in practical applications due to the introduction of various defects. Here, a novel method based on strain engineering is employed for achieving superlubricity in the DWCNT using molecular dynamics simulations. The DWCNT exhibits a superlow friction force when an inner tube slides against a stretched outer tube even with a low content of defects. However, strain engineering shows its limitation on superlubricity in the case of a large magnitude of strain or a high content of point defects. The mechanism of superlubricity in the DWCNT could be explained by the analysis of the energy barrier.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cp06052fDOI Listing
March 2021

Water-soluble green-emitting carbon nanodots with enhanced thermal stability for biological applications.

Nanoscale 2021 Feb;13(7):4301-4307

National and Local Joint Engineering Laboratory of Optical-Conversion Materials and Technology & School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, P.R. China.

High stability and water solubility of fluorescent nanomaterials are considered key factors to evaluate their feasibility for fundamental applications. Herein, water-soluble and thermally stable, green-emitting carbon nanodots (CNDs) have been synthesized via a facile hydrothermal method with an average size of 1.9 nm. CNDs showed green emission centered at 544 nm with the photo-luminescence quantum yield (PLQY) of up to 10.1% under the excitation of 400 nm. The obtained CNDs demonstrated high resistance towards photo-bleaching and an ionic (KCl) environment. Moreover, the aqueous solution of CNDs exhibited excellent stability under harsh thermal conditions from 10 °C to 80 °C. The as-prepared CNDs showed stable performance at high temperatures, even after keeping them at 80 °C for 30 min. Furthermore, the green emissive CNDs were incubated in T-ca cancer cells for bio-imaging applications. The results indicated that CNDs can served as an effective thermally-stable bio-imaging agent in T-ca cells at the physiological temperature range of 25 °C-45 °C. Green emission and excellent thermal stability make these CNDs promising fluorescent materials for potential applications in the medical field, which requires long-wavelength fluorescence and high-temperature imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr09131fDOI Listing
February 2021

Ionic Transport and Sieving Properties of Sub-nanoporous Polymer Membranes with Tunable Channel Size.

ACS Appl Mater Interfaces 2021 Feb 15;13(7):9015-9026. Epub 2021 Feb 15.

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China.

Bioinspired nanoporous membranes show great potential in ionic separation and water filtration by offering high selectivity with less permeation resistance. However, complex processes always limit their applications. Here, we report a convenient approach to introduce ionic selective channels in a micron-thick polycarbonate membrane through swift heavy ion irradiation accompanied by UV sensitization and pulsed-electrical etching. The characteristic dimension of channels was tuned through regulating energy loss of the incident ion and UV sensitization time of the membrane, resulting in the sub-nanoporous membranes with mean channel diameter ranging from <2.4 to 9.7 Å. These membranes showed the voltage-activated ionic transport properties associated with the dehydration effect, and the corresponding - characteristics were related to ionic strength, solution pH, ionic type, and channel diameter. It was found that the transmembrane conduction of multivalent ions was severely suppressed compared to monovalent ions, until the size of the membrane channel was comparable to the hydrated diameter of multivalent ions. Ionic sieving experiments also demonstrated the excellent ionic valence selectivity of the membrane. Even for the membrane with a channel diameter close to 1 nm, the Li/Mg separation ratio was still as high as 40, and an even higher separation ratio was found for Li/La (>3000).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c22689DOI Listing
February 2021

Multi-Scale Frequency Bands Ensemble Learning for EEG-Based Emotion Recognition.

Sensors (Basel) 2021 Feb 10;21(4). Epub 2021 Feb 10.

School of Computer Science and Technology, Hangzhou Dianzi University, Hangzhou 310018, China.

Emotion recognition has a wide range of potential applications in the real world. Among the emotion recognition data sources, electroencephalography (EEG) signals can record the neural activities across the human brain, providing us a reliable way to recognize the emotional states. Most of existing EEG-based emotion recognition studies directly concatenated features extracted from all EEG frequency bands for emotion classification. This way assumes that all frequency bands share the same importance by default; however, it cannot always obtain the optimal performance. In this paper, we present a novel multi-scale frequency bands ensemble learning (MSFBEL) method to perform emotion recognition from EEG signals. Concretely, we first re-organize all frequency bands into several local scales and one global scale. Then we train a base classifier on each scale. Finally we fuse the results of all scales by designing an adaptive weight learning method which automatically assigns larger weights to more important scales to further improve the performance. The proposed method is validated on two public data sets. For the "SEED IV" data set, MSFBEL achieves average accuracies of 82.75%, 87.87%, and 78.27% on the three sessions under the within-session experimental paradigm. For the "DEAP" data set, it obtains average accuracy of 74.22% for four-category classification under 5-fold cross validation. The experimental results demonstrate that the scale of frequency bands influences the emotion recognition rate, while the global scale that directly concatenating all frequency bands cannot always guarantee to obtain the best emotion recognition performance. Different scales provide complementary information to each other, and the proposed adaptive weight learning method can effectively fuse them to further enhance the performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21041262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916620PMC
February 2021

Extracorporeal Membrane Oxygenation for SARS-CoV-2 Acute Respiratory Distress Syndrome: A Retrospective Study From Hubei, China.

Front Med (Lausanne) 2020 12;7:611460. Epub 2021 Jan 12.

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The data on long-term outcomes of patients infected by SARS-CoV-2 and treated with extracorporeal membrane oxygenation (ECMO) in China are merely available. A retrospective study included 73 patients infected by SARS-CoV-2 and treated with ECMO in 21 intensive care units in Hubei, China. Data on demographic information, clinical features, laboratory tests, ECMO durations, complications, and living status were collected. The 73 ECMO-treated patients had a median age of 62 (range 33-78) years and 42 (63.6%) were males. Before ECMO initiation, patients had severe respiratory failure on mechanical ventilation with a median PO/FiO of 71.9 [interquartile range (IQR), 58.6-87.0] mmHg and a median PCO of 62 [IQR, 43-84] mmHg on arterial blood analyses. The median duration from symptom onset to invasive mechanical ventilation, and to ECMO initiation was19 [IQR, 15-25] days, and 23 [IQR, 19-31] days. Before and after ECMO initiation, the proportions of patients receiving prone position ventilation were 58.9 and 69.9%, respectively. The median duration of ECMO support was 18.5 [IQR 12-30] days. During the treatments with ECMO, major hemorrhages occurred in 31 (42.5%) patients, and oxygenators were replaced in 21 (28.8%) patients. Since ECMO initiation, the 30-day mortality and 60-day mortality were 63.0 and 80.8%, respectively. In Hubei, China, the ECMO-treated patients infected by SARS-CoV-2 were of a broad age range and with severe hypoxemia. The durations of ECMO support, accompanied with increased complications, were relatively long. The long-term mortality in these patients was considerably high.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2020.611460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835137PMC
January 2021

Controlling Nutritional Status (CONUT) Score as a New Indicator of Prognosis in Patients With Hilar Cholangiocarcinoma Is Superior to NLR and PNI: A Single-Center Retrospective Study.

Front Oncol 2020 11;10:593452. Epub 2021 Jan 11.

Department of General Surgery, Nanchong Central Hospital, The Second Clinical College of North Sichuan Medical College, Nanchong, China.

Background: Currently, many nutritional indicators, including controlling nutritional status score (CONUT), can be used to assess a patient's nutritional status and have been reported as reliable predictors of multiple malignancies. However, the value of CONUT score in predicting postoperative outcomes in patients with hilar cholangiocarcinoma has not been explored. In this study, its predictive value will be discussed and compared with the known predictors the neutrophil lymphocyte ratio (NLR) and prognostic nutritional index (PNI).

Methods: Preoperative CONUT scores, PNI and NLR levels of 94 Hilar cholangiocarcinoma (HCCA) patients who underwent radical-intent resection of hepatobiliary surgery in our hospital from March 2010 to April 2019 were retrospectively collected and analyzed. They were grouped according to their optimal cutoff value and the prognostic effects of patients in each group were compared respectively.

Results: CONUT was more frequent in patients with Clavien-Dindo classification of ≥IIIa (P = 0.008) and Bile leakage presence (P = 0.011). Kaplan-Meier curves analyzing the relationship between CONUT, PNI, and NLR values and HCCA patient survival (including total survival (OS) and recurrence-free survival (RFS) showed significant differences between groups (P <0.001). Meanwhile, multi-factor analysis found that Degree of cure, PNI, NLR, and preoperative CONUT score were independent prognostic factors for OS and RFS. The predictive power of CONUT score was higher than that of NLR and PNI based on time-dependent receiver operating Characteristic (ROC) analysis and the net reclassification index (NRI) and integrated discriminatory index (IDI) values (P < 0.05).

Conclusion: CONUT score may be of some clinical reference value in evaluating postoperative prognosis of HCCA patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.593452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829909PMC
January 2021

Publisher Correction: A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity.

Nature 2021 Feb;590(7844):E23

Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41586-020-03108-4DOI Listing
February 2021

Cytoplasmic SHMT2 drives the progression and metastasis of colorectal cancer by inhibiting β-catenin degradation.

Theranostics 2021 1;11(6):2966-2986. Epub 2021 Jan 1.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China.

Serine hydroxymethyltransferase 2 (SHMT2) plays a critical role in serine-glycine metabolism to drive cancer cell proliferation. However, the nonmetabolic function of SHMT2 in tumorigenesis, especially in human colorectal cancer (CRC) progression, remains largely unclear. SHMT2 expression in human CRC cells was identified by western blot and immunofluorescence assay. The CRC cell proliferation, migration, and invasion after SHMT2 knockdown or overexpression were explored through and assays. Immunofluorescence, mRNA-seq, co-immunoprecipitation, chromatin immunoprecipitation-qPCR and immunohistochemistry assays were used to investigate the underlying mechanisms behind the SHMT2 nonmetabolic function. We demonstrated that SHMT2 was distributed in the cytoplasm and nucleus of human CRC cells. SHMT2 knockdown resulted in the significant inhibition of CRC cell proliferation, which was not restored by serine, glycine, or formate supplementation. The invasion and migration of CRC cells were suppressed after SHMT2 knockdown. Mechanistically, SHMT2 interacted with β-catenin in the cytoplasm. This interaction inhibited the ubiquitylation-mediated degradation of β-catenin and subsequently modulated the expression of its target genes, leading to the promotion of CRC cell proliferation and metastasis. Notably, the lysine 64 residue on SHMT2 (SHMT2) mediated its interaction with β-catenin. Moreover, transcription factor TCF4 interacted with β-catenin, which in turn increased expression, forming an SHMT2/β-catenin positive feedback loop. xenograft experiments confirmed that SHMT2 promoted the growth and metastasis of CRC cells. Finally, the level of SHMT2 was found to be significantly increased in human CRC tissues. The SHMT2 level was correlated with an increased level of β-catenin, associated with CRC progression and predicted poor patient survival. Taken together, our findings reveal a novel nonmetabolic function of SHMT2 in which it stabilizes β-catenin to prevent its ubiquitylation-mediated degradation and provide a potential therapeutic strategy for CRC therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.48699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806468PMC
January 2021

CircBA1 derived from BCR-ABL fusion gene inhibits cell proliferation in chronic myeloid leukemia.

Cancer Commun (Lond) 2021 Jan 14;41(1):79-82. Epub 2021 Jan 14.

Department of Gastroenterology and Hepatology, West China Hospital of Sichuan University, Chengdu, Sichuan, 610041, P. R. China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cac2.12120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819548PMC
January 2021

Effect of Linker Distribution in the Photocatalytic Activity of Multivariate Mesoporous Crystals.

J Am Chem Soc 2021 02 12;143(4):1798-1806. Epub 2021 Jan 12.

Functional Inorganic Materials Team, Instituto de Ciencia Molecular (ICMol), Universitat de València, Paterna, 46980 València, Spain.

The use of Metal-Organic Frameworks as crystalline matrices for the synthesis of multiple component or multivariate solids by the combination of different linkers into a single material has emerged as a versatile route to tailor the properties of single-component phases or even access new functions. This approach is particularly relevant for Zr-MOFs due to the synthetic flexibility of this inorganic node. However, the majority of materials are isolated as polycrystalline solids, which are not ideal to decipher the spatial arrangement of parent and exchanged linkers for the formation of homogeneous structures or heterogeneous domains across the solid. Here we use high-throughput methodologies to optimize the synthesis of single crystals of UiO-68 and UiO-68-TZDC, a photoactive analogue based on a tetrazine dicarboxylic derivative. The analysis of the single linker phases reveals the necessity of combining both linkers to produce multivariate frameworks that combine efficient light sensitization, chemical stability, and porosity, all relevant to photocatalysis. We use solvent-assisted linker exchange reactions to produce a family of UiO-68-TZDC binary frameworks, which respect the integrity and morphology of the original crystals. Our results suggest that the concentration of TZDC in solution and the reaction time control the distribution of this linker in the sibling crystals for a uniform mixture or the formation of core-shell domains. We also demonstrate how the possibility of generating an asymmetric distribution of both linkers has a negligible effect on the electronic structure and optical band gap of the solids but controls their performance for drastic changes in the photocatalytic activity toward proton or methyl viologen reduction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c09015DOI Listing
February 2021

Novel Recurrent Altered Genes in Chinese Patients With Anaplastic Thyroid Cancer.

J Clin Endocrinol Metab 2021 Mar;106(4):988-998

Department of Thyroid and Parathyroid Surgery, Laboratory of thyroid and parathyroid disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: Anaplastic thyroid cancer (ATC) is a rare but lethal malignancy, and few systematic investigations on genomic profiles of ATC have been performed in Chinese patients.

Methods: Fifty-four ATC patients in West China Hospital between 2010 to 2020 were retrospectively analyzed, while 29 patients with available samples were sequenced by whole-exome sequencing (WES). The associations between genomic alterations and clinical characteristics were statistically evaluated.

Results: The median overall survival was 3.0 months in the entire cohort, which was impacted by multiple clinical features, including age, tumor size, and different treatment strategies. In the WES cohort, totally 797 nonsilent mutations were detected; the most frequently altered genes were TP53 (48%), BRAF (24%), PIK3CA (24%), and TERT promoter (21%). Although these mutations have been well-reported in previous studies, ethnic specificity was exhibited in terms of mutation frequency. Moreover, several novel significantly mutated genes were identified including RBM15 (17%), NOTCH2NL (14%), CTNNA3 (10%), and KATNAL2 (10%). WES-based copy number alteration analysis also revealed a high frequent gain of NOTCH2NL (41%), which induced its increased expression. Gene mutations and copy number alterations were enriched in phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin (mTOR), NOTCH, and WNT pathways.

Conclusions: This study reveals shared and ethnicity-specific genomic profiles of ATC in Chinese patients and suggests NOTCH2NL may act as a novel candidate driver gene for ATC tumorigenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1210/clinem/dgab014DOI Listing
March 2021

Case Report: ST-Segment Elevation in a Man With Acute Pericarditis.

Front Cardiovasc Med 2020 23;7:609691. Epub 2020 Dec 23.

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.

Acute pericarditis is a rapid inflammatory condition of the pericardium with both infectious and non-infectious etiology. Most acute pericarditis is self-limited, with a small portion evolving rapidly. The definitive diagnosis of acute pericarditis often requires detailed physical examination, ECG, echocardiography, blood analysis and chest X-ray. It's usually challenging to distinguish acute pericarditis from ST-elevated myocardial infarction (STEMI) due to the similar ECG characteristics (ST segment change). Here we present a case of purulent pericarditis probably caused by esophageal perforation. A 52 year-old male presented with chest pain and dyspnea for 16 h. ST-segment elevation and positive cardiac markers lead to the initial diagnosis of ST-elevated myocardial infarction. Coronary angiography demonstrated normal coronary artery, while transthoracic echocardiography (TTE) showed massive pericardial effusion. Then, pericardiocentesis was performed with 250 ml of yellowish-green pus-like fluid extracted. A detailed history examination revealed a week history of possible esophageal perforation caused by a fishbone. And a further computed tomography (CT) demonstrated the presence of pneumomediastinum, and effusions in mediastinum, which lead to the diagnosis of purulent pericarditis. However, the patient's family refused further treatment and the patient died soon after discharge. The differential diagnosis of chest pain should include acute pericarditis, which can be equally critical and fatal. And it's important to note the peculiar characteristics of acute pericarditis, which include concave and diffused ST-segment elevation, PR segment depression, and the ratio of ST-segment elevation to T wave >0.24 in lead V6. Moreover, comprehensive medical history and physical examination are crucial to the differential diagnosis of chest pain patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2020.609691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793765PMC
December 2020

Tracheostomy in 80 COVID-19 Patients: A Multicenter, Retrospective, Observational Study.

Front Med (Lausanne) 2020 17;7:615845. Epub 2020 Dec 17.

Department of Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.

The outbreak of coronavirus disease 2019 (COVID-19) has led to a large and increasing number of patients requiring prolonged mechanical ventilation and tracheostomy. The indication and optimal timing of tracheostomy in COVID-19 patients are still unclear, and the outcomes about tracheostomy have not been extensively reported. We aimed to describe the clinical characteristics and outcomes of patients with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia who underwent elective tracheostomies. The multi-center, retrospective, observational study investigated all the COVID-19 patients who underwent elective tracheostomies in intensive care units (ICUs) of 23 hospitals in Hubei province, China, from January 8, 2020 to March 25, 2020. Demographic information, clinical characteristics, treatment, details of the tracheostomy procedure, successful weaning after tracheostomy, and living status were collected and analyzed. Data were compared between early tracheostomy patients (tracheostomy performed within 14 days of intubation) and late tracheostomy patients (tracheostomy performed after 14 days). A total of 80 patients were included. The median duration from endotracheal intubation to tracheostomy was 17.5 [IQR 11.3-27.0] days. Most tracheotomies were performed by ICU physician [62 (77.5%)], and using percutaneous techniques [63 (78.8%)] at the ICU bedside [76 (95.0%)]. The most common complication was tracheostoma bleeding [14 (17.5%)], and major bleeding occurred in 4 (5.0%) patients. At 60 days after intubation, 31 (38.8%) patients experienced successful weaning from ventilator, 17 (21.2%) patients discharged from ICU, and 43 (53.8%) patients had died. Higher 60 day mortality [22 (73.3%) vs. 21 (42.0%)] were identified in patients who underwent early tracheostomy. In patients with SARS-CoV-2 pneumonia, tracheostomies were feasible to conduct by ICU physician at bedside with few major complications. Compared with tracheostomies conducted after 14 days of intubation, tracheostomies within 14 days were associated with an increased mortality rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2020.615845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793766PMC
December 2020