Publications by authors named "Yong Jie Andrew Chan"

2 Publications

  • Page 1 of 1

Environmental stresses induce karyotypic instability in colorectal cancer cells.

Mol Biol Cell 2019 01 31;30(1):42-55. Epub 2018 Oct 31.

Institute of Medical Biology, Singapore 138648, Republic of Singapore.

Understanding how cells acquire genetic mutations is a fundamental biological question with implications for many different areas of biomedical research, ranging from tumor evolution to drug resistance. While karyotypic heterogeneity is a hallmark of cancer cells, few mutations causing chromosome instability have been identified in cancer genomes, suggesting a nongenetic origin of this phenomenon. We found that in vitro exposure of karyotypically stable human colorectal cancer cell lines to environmental stress conditions triggered a wide variety of chromosomal changes and karyotypic heterogeneity. At the molecular level, hyperthermia induced polyploidization by perturbing centrosome function, preventing chromosome segregation, and attenuating the spindle assembly checkpoint. The combination of these effects resulted in mitotic exit without chromosome segregation. Finally, heat-induced tetraploid cells were on the average more resistant to chemotherapeutic agents. Our studies suggest that environmental perturbations promote karyotypic heterogeneity and could contribute to the emergence of drug resistance.
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http://dx.doi.org/10.1091/mbc.E18-10-0626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337910PMC
January 2019

Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis.

Sci Rep 2018 02 13;8(1):2890. Epub 2018 Feb 13.

Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Polyploidization, a common event during the evolution of different tumours, has been proposed to confer selective advantages to tumour cells by increasing the occurrence of mutations promoting cancer progression and by conferring chemotherapy resistance. While conditions leading to polyploidy in cancer cells have been described, a general mechanism explaining the incidence of this karyotypic change in tumours is still missing. In this study, we tested whether a widespread tumour microenvironmental condition, low pH, could induce polyploidization in mammalian cells. We found that an acidic microenvironment, in the range of what is commonly observed in tumours, together with the addition of lactic acid, induced polyploidization in transformed and non-transformed human cell lines in vitro. In addition, we provide evidence that polyploidization was mainly driven through the process of endoreduplication, i.e. the complete skipping of mitosis in-between two S-phases. These findings suggest that acidic environments, which characterize solid tumours, are a plausible path leading to polyploidization of cancer cells.
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http://dx.doi.org/10.1038/s41598-018-20186-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811548PMC
February 2018