Publications by authors named "Yong Jiang"

1,507 Publications

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Thrombolysis, time-to-treatment and in-hospital outcomes among young adults with ischaemic stroke in China: findings from a nationwide registry study in China.

BMJ Open 2022 Jun 24;12(6):e055055. Epub 2022 Jun 24.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Background And Purpose: We aimed to determine whether young adults (<50 years) with acute ischaemic stroke (AIS) are more likely to receive intravenous tissue plasminogen activator (IV tPA) and have shorter time to treatment than older patients with stroke.

Methods: We analysed data from the Chinese Stroke Center Alliance registry for patients with AIS hospitalised between August 2015 and July 2019. Patients were classified into two groups according to age: young adults (<50 years of age) and older adults (≥50 years of age).

Results: Of 793 175 patients with AIS admitted to 1471 hospitals, 9.1% (71 860) were young adults. Compared with older adults, a higher proportion of young adults received IV tPA among patients without contraindicaitons (7.2% vs 6.1%, adjusted OR (aOR) 1.13, 95% CI 1.10 to 1.17) and among patients without contraindications and with onset-to-door time ≤3.5 hours (23.6% vs 19.3%, aOR 1.20, 95% CI 1.15 to 1.24). We did not observe differences in onset-to-needle time (median hours 2.7 hours) or door-to-needle time (DNT) (median minutes 60 min) between young and older adults. The proportion of DNT ≤30 min, DNT ≤45 min and DNT ≤60 min in young and older IV tPA-treated patients were 16.9% vs 18.8%, 30.2% vs 32.8% and 50.2% vs 54.2%, respectively. Compared with older adults, young adults treated with IV tPA had lower odds of in-hospital mortality (0.5% vs 1.3%, aOR 0.54, 95% CI 0.35 to 0.82) and higher odds of independent ambulation at discharge (61.0% vs 53.6%, aOR 1.15, 95% CI 1.08 to 1.22), and the associations may be partly explained by stroke severity measured by the National Institutes of Health Stroke Scale score.

Conclusion: Young adults with AIS were more likely to receive IV tPA than older adults, although there was no difference between the two groups in time to treatment. Compared with older adults, young adults may had better in-hospital outcomes.
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http://dx.doi.org/10.1136/bmjopen-2021-055055DOI Listing
June 2022

Variations in Marine Bacterial and Archaeal Communities during an Green Tide in Coastal Qingdao Areas.

Microorganisms 2022 Jun 13;10(6). Epub 2022 Jun 13.

College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China.

Green tides caused by occur annually in the Yellow Sea, potentially influencing the marine microorganisms. Here, we focused on the variations in marine bacterial and archaeal communities during an green tide in coastal Qingdao areas with Illumina high-throughput sequencing analysis. Our results revealed that the diversity and structure of bacterial and archaeal communities, as well as the organization and structure of microbial co-occurrence networks, varied during the green tide. The decline phase may be favorable to the bacterial and archaeal diversity and richness. The bacterial community, as well as the archaeal community, showed clear variations between the outbreak and decline phases. A simpler and less connected microbial co-occurrence network was observed during the outbreak phase compared with the decline phase. and separately dominated the bacterial community during the outbreak and decline phase, and () dominated the archaeal community during the green tide. Combined with microbial co-occurrence network analysis, , and may be important organisms during the green tide. Temperature, chlorophyll content and salinity may have an important impact on the variations in bacterial and archaeal communities during the green tide.
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http://dx.doi.org/10.3390/microorganisms10061204DOI Listing
June 2022

Genome-Wide Association Study of Potential Meat Quality Trait Loci in Ducks.

Genes (Basel) 2022 May 31;13(6). Epub 2022 May 31.

College of Animal Science, Technology of Yangzhou University, Yangzhou 225009, China.

With continuously increasing living standards and health requirements of consumers, meat quality is becoming an important consideration while buying meat products. To date, no genome-wide association study (GWAS) for copy number variants (CNVs) and single nucleotide polymorphisms (SNPs) has been conducted to reveal the genetic effects on meat quality in ducks. This study analyzed the phenotypic correlation and heritability of fat, water, collagen, and protein content of duck breast muscle. To identify the candidate variants for meat quality, we performed a GWAS using 273 ducks from an F2 population. The results of the SNP GWAS showed that the , , and genes were associated with fat content; , , and with water content; , , , and with collagen production; and , , and with protein content. Additionally, 3, 7, 1, and 3 CNVs were associated with fat, water, collagen, and protein content, respectively, in duck breast muscle. The genes identified in this study can serve as markers for meat quality. Furthermore, our findings may help devise effective breeding plans and selection strategies to improve meat quality.
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http://dx.doi.org/10.3390/genes13060986DOI Listing
May 2022

Genome-Wide Association Study of Feed Efficiency Related Traits in Ducks.

Animals (Basel) 2022 Jun 13;12(12). Epub 2022 Jun 13.

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Feed efficiency (FE) is the most important economic trait in the poultry and livestock industry. Thus, genetic improvement of FE may result in a considerable reduction of the cost and energy burdens. As genome-wide association studies (GWASs) can help identify candidate variants influencing FE, the present study aimed to analyze the phenotypic correlation and identify candidate variants of the seven FE traits in ducks. All traits were found to have significant positive correlations with varying degrees. In particular, residual feed intake presented correlation coefficients of 0.61, 0.54, and 0.13 with feed conversion ratio, and feed intake, respectively. Furthermore, data from seven FE-related GWAS revealed 4 (FCR), 3 (FI), 36 (RFI), 6 (BWG), 8 (BW21), and 10 (BW42) SNPs were significantly associated with body weight gain, feed intake, residual feed intake, feed conversion ratio, and weight at 21 and 42 days, respectively. Candidate SNPs of seven FE trait-related genes were involved in galactose metabolism, starch, propanoate metabolism, sucrose metabolism and etc. Taken together, these findings provide insight into the genetic mechanisms and genes involved in FE-related traits in ducks. However, further investigations are warranted to further validate these findings.
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http://dx.doi.org/10.3390/ani12121532DOI Listing
June 2022

The combined effects of microplastics and the heavy metal cadmium on the marine periphytic ciliate Euplotes vannus.

Environ Pollut 2022 Jun 20:119663. Epub 2022 Jun 20.

College of Marine Life Science, Ocean University of China, Qingdao, 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China. Electronic address:

Microplastics could be grazed by marine organisms and possibly transferred to higher trophic levels along the microbial loop. Due to their size and capacity to concentrate heavy metals that trigger joint toxic effects, microplastics (MPs) have already become a severe threat to marine organisms. The detrimental effects of MPs on large marine organisms have been studied, but the combined toxicity of MPs and cadmium (Cd) on protozoan ciliates remains unclear. In the present study, we selected different diameters and concentrations of polystyrene microspheres (PS-MPs) and Cd as model MPs and heavy metals to evaluate their single and combined effects on the periphytic marine ciliate Euplotes vannus in relation to carbon biomass and oxidative stress. The MPs were indeed ingested by Euplotes vannus and significantly reduced the abundance and carbon biomass of ciliate populations. Combined exposure to MPs and Cd not only increased the bioaccumulation of Cd in ciliates but also exacerbated the decrease in ciliate biomass by increasing oxidative stress and membrane damage. In comparison, the effects of nano-sized plastics (0.22 μm) were more harmful than those of micro-sized plastics (1.07 μm, 2.14 μm and 5.00 μm). A smaller size represents a higher potential for penetrating biological members and a stronger adsorption capacity for cadmium. These results provide new insight into the combined toxicity of microplastics and heavy metals on ciliated protozoa and lay a foundation for higher trophic levels and ecosystems.
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http://dx.doi.org/10.1016/j.envpol.2022.119663DOI Listing
June 2022

Backdoor Learning: A Survey.

IEEE Trans Neural Netw Learn Syst 2022 Jun 22;PP. Epub 2022 Jun 22.

Backdoor attack intends to embed hidden backdoors into deep neural networks (DNNs), so that the attacked models perform well on benign samples, whereas their predictions will be maliciously changed if the hidden backdoor is activated by attacker-specified triggers. This threat could happen when the training process is not fully controlled, such as training on third-party datasets or adopting third-party models, which poses a new and realistic threat. Although backdoor learning is an emerging and rapidly growing research area, there is still no comprehensive and timely review of it. In this article, we present the first comprehensive survey of this realm. We summarize and categorize existing backdoor attacks and defenses based on their characteristics, and provide a unified framework for analyzing poisoning-based backdoor attacks. Besides, we also analyze the relation between backdoor attacks and relevant fields (i.e., adversarial attacks and data poisoning), and summarize widely adopted benchmark datasets. Finally, we briefly outline certain future research directions relying upon reviewed works. A curated list of backdoor-related resources is also available at https://github.com/THUYimingLi/backdoor-learning-resources.
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http://dx.doi.org/10.1109/TNNLS.2022.3182979DOI Listing
June 2022

[Changes of non-structural carbohydrates and nitrogen contents of needles and twigs in var. plantations along an aridity gradient].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1518-1524

College of Life Sciences, Tianjin Normal University, Tianjin 300387, China.

With six var. plantations (Huinan, Xifeng, Fujia, Zhanggutai, Naiman and Wulanaodu) along an aridity gradient in the Horqin sandy land, we examined the changes in non-structural carbohydrates (NSCs) and nitrogen (N) contents of current and one-year-old needles and twigs, to explore the carbon supply and demand status as well as the nutrient accumulation strategies of var. under drought. The results showed that the contents of NSCs and soluble sugars in needles and twigs of var. plantations significantly decreased with increasing aridity. From the most humid site (Huinan) to the most aridity site (Wulanaodu), the soluble sugar contents in current and one-year old needles of var. decreased from 12.8% and 12.5% to 9.0% and 9.5%, respectively. The soluble sugar contents in current-year old twigs decreased from 15.6% to 9.2%. With increasing aridity, the starch contents in needles and twigs remained relatively stable, soluble sugars/starch ratio in current and one-year old needles decreased, the N contents in current and one-year old twigs significantly increased. The var. plantations in the Horqin sandy land consumed soluble sugar storage under drought, resulting in a risk of mortality from 'carbon starvation'. var. tended to maintain stable starch storage and accumulate N in twigs to cope with long-term drought stress.
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http://dx.doi.org/10.13287/j.1001-9332.202206.005DOI Listing
June 2022

Time Course for Benefit and Risk With Ticagrelor and Aspirin in Individuals With Acute Ischemic Stroke or Transient Ischemic Attack Who Carry CYP2C19 Loss-of-Function Alleles: A Secondary Analysis of the CHANCE-2 Randomized Clinical Trial.

JAMA Neurol 2022 Jun 21. Epub 2022 Jun 21.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Importance: Dual antiplatelet therapy (DAPT) with ticagrelor and aspirin has been found to be effective for secondary prevention after minor ischemic stroke or transient ischemic attack (TIA) in individuals who carry CYP2C19 loss-of-function (LOF) alleles; however, uncertainties remain about the time course of benefit and risk with ticagrelor and aspirin in these patients.

Objective: To obtain time-course estimates of efficacy and risk with ticagrelor and aspirin after minor stroke or TIA in individuals with CYP2C19 LOF alleles.

Design, Setting, And Participants: The Ticagrelor or Clopidogrel With Aspirin in High-risk Patients With Acute Nondisabling Cerebrovascular Events II (CHANCE-2) randomized clinical trial enrolled patients 40 years and older from 202 hospitals in China with acute minor stroke or TIA who carried CYP2C19 LOF alleles between September 23, 2019, and March 22, 2021, and were followed up for 90 days. All 6412 patients enrolled in the CHANCE-2 trial were included in this secondary analysis. Data were analyzed in October 2021.

Interventions: Ticagrelor (180 mg on day 1 followed by 90 mg twice daily on days 2-90) or clopidogrel (300 mg on day 1 followed by 75 mg daily on days 2-90). All patients received aspirin (75-300 mg on day 1 followed by 75 mg daily for 21 days).

Main Outcomes And Measures: The efficacy outcome was major ischemic event, defined as the composite of ischemic stroke or nonhemorrhagic death. Safety outcomes included moderate to severe bleeding and any bleeding.

Results: A total of 6412 patients were included (3205 in the ticagrelor and aspirin group and 3207 in the clopidogrel and aspirin group). The median (IQR) age was 65 (57-71) years, and 4242 patients (66%) were men. The reduction of major ischemic events with ticagrelor and aspirin predominately occurred in the first week (absolute risk reduction, 1.34%; 95% CI, 0.29 to 2.39) and attenuated but remained in the next 3 weeks (absolute risk reduction in the second week, 0.11%; 95% CI, -0.24 to 0.45; absolute risk reduction in the third week, 0.14%; 95% CI, -0.11 to 0.38; absolute risk reduction in the fourth week, 0.04%; 95% CI, -0.18 to 0.25). The risk of moderate to severe bleeding was consistently low in the ticagrelor and aspirin group. The absolute increase in any bleeding seen in the first week (0.87%; 95% CI, 0.25 to 1.50) remained in the next 3 weeks (absolute increase in the second week, 1.21%; 95% CI, 0.75 to 1.68; absolute increase in the third week, 0.33%; 95% CI, -0.05 to 0.72; absolute increase in the fourth week, 0.23%; 95% CI, -0.03 to 0.49).

Conclusion And Relevance: Among patients with minor stroke or TIA who carried CYP2C19 LOF alleles, benefit with ticagrelor and aspirin was present predominately in the first week, with additional small benefit accruing in the next 2 weeks.
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http://dx.doi.org/10.1001/jamaneurol.2022.1457DOI Listing
June 2022

Short-term effect of PM2.5 on stroke in susceptible populations: a case-crossover study.

Int J Stroke 2022 Jun 19:17474930221110024. Epub 2022 Jun 19.

Vascular Neurology, Department of Neurology, Beijing TianTan Hospital, Capital Medical University, Beijing, China 105738.

Background: Fine particulate matter (PM2.5) is a risk factor for stroke, and patients with pre-existing diseases appear to be particularly susceptible. We conducted a case-crossover study to examine the association between short-term exposure to fine particulate matter (PM2.5) and hospital admission for stroke in individuals with atrial fibrillation (AF), hypertension, diabetes, or hyperlipidemia.

Methods: Patients diagnosed with acute ischemic stroke (AIS) were recruited from 2015 to 2017 in Chinese Stroke Center Alliances. We estimated daily PM2.5 average exposures with a spatial resolution of 0.1° using a data assimilation approach combining satellite measurements, air model simulations, and monitoring values. Conditional logistic regression was used to assess PM2.5-related stroke risk in patients with pre-existing medical co-morbidities.

Results: A total of 155 616 patients diagnosed with AIS were admitted. Patients with a history of AF (n=15 430), hypertension (n=138 220), diabetes (n=43 737), or hyperlipidemia (n=16 855) were assessed separately. A 10 µg/m3 increase in daily PM2.5 was associated with a significant increase in AIS for individuals with AF at lag 4 (OR, 1.008; 95% CI, 1.002-1.014), and with hypertension (OR, 1.008; 95% CI, 1.006-1.010), diabetes (OR, 1.006; 95% CI, 1.003-1.010), and hyperlipidemia (OR, 1.007; 95% CI, 1.001-1.012) at lag 0-7. Elderly (≥65 years old) and female patients with AF had significantly higher associations at lag 5 (OR, 1.009; 95% CI, 1.002-1.015) and lag 5 (OR, 1.010; 95% CI, 1.002-1.018), respectively.

Conclusions: Short-term exposure to PM2.5 is significantly associated with hospital admission for stroke in individuals with pre-existing medical histories, especially in older or female patients with AF. Preventative measures to reduce PM2.5 concentrations are particularly important in individuals with other medical co-morbidities.
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http://dx.doi.org/10.1177/17474930221110024DOI Listing
June 2022

Microplastics shift macrobenthic community structures near a coastal nuclear power plant under construction in North East China.

J Hazard Mater 2022 Jun 10;437:129335. Epub 2022 Jun 10.

College of Marine Life Sciences, and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address:

Coastal sediments are considered to be a potential sink for microplastics, which mainly derived from the land-based sources. This study investigated microplastic pollution in the sediments of 20 stations near a nuclear power plant under construction in North East China and analyzed its impacts on macrobenthic communities. The average abundance of microplastics in three stations close to the nuclear power plant was 0.33 items/g, which was approximately 10 times higher than those in the distant stations. The clustering of microplastic characteristics (sizes, shapes, and colors) showed that the three stations near the plant were in one group, and the distant stations were in another group. A total of 105 macroinvertebrate species belonging to 7 phyla were identified in all stations, and the dominant phyla were Annelida and Arthropoda. Spearman rank correlation showed that rare taxa (83 species with a contribution <1 %) were positively correlated with microplastics and As, and redundancy analysis demonstrated that the distribution patterns of macrobenthic communities were similar to those of microplastics. Moreover, co-occurrence networks showed that the rare taxa were positively correlated with microplastics. Therefore, microplastics released from the nuclear power plant under construction might shift the structure of macrobenthic communities, especially the rare taxa.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129335DOI Listing
June 2022

Association between haemoglobin A1c and cerebral microbleeds in community-based stroke-free individuals: A cross-sectional study.

Diabetes Metab Res Rev 2022 Jun 10:e3557. Epub 2022 Jun 10.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Aims: The association between haemoglobin A1c (HbA1c) and cerebral microbleeds (CMBs) remains unclear. We aimed to investigate the association between HbA1c and CMBs in community-based individuals without stroke or transient ischaemic attack (TIA) and whether the association differs between individuals with and without diabetes mellitus (DM).

Materials And Methods: All individuals were recruited from a community in Beijing, China, from January 2015 to September 2019. All individuals completed a questionnaire and underwent blood tests and brain magnetic resonance imaging. A susceptibility-weighted imaging sequence was acquired to detect CMBs, which were defined as small, round and low-signal lesions with <10 mm diameter. The association between HbA1c and CMBs was analysed using multivariable logistic regression adjusted for demographics, medical history and blood sample test results. Subgroup analyses stratified by history of DM were performed.

Results: Of 544 recruited individuals, 119 (21.88%) had CMBs. HbA1c was independently associated with CMBs (odds ratio [OR], 1.51; 95% confidence interval [CI], 1.03-2.22). In 87 individuals with DM, multivariable logistic analysis showed that HbA1c was significantly associated with CMBs (OR, 1.67; 95% CI, 1.04-2.69), whereas in individuals without DM, no significant association was observed between HbA1c and CMBs (OR, 1.07; 95% CI, 0.50-2.30).

Conclusions: HbA1c was associated with CMBs in individuals without stroke or TIA, particularly in individuals with DM, suggesting that the status of glycaemic control warrants attention for the prevention of CMBs. It would be beneficial to manage HbA1c specifically to control the risk of CMBs, especially in individuals with DM.
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http://dx.doi.org/10.1002/dmrr.3557DOI Listing
June 2022

Rapamycin Attenuates Anxiety and Depressive Behavior Induced by Helicobacter pylori in Association with Reduced Circulating Levels of Ghrelin.

Neural Plast 2022 30;2022:2847672. Epub 2022 May 30.

Department of Gastroenterology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Background: Helicobacter pylori (H. pylori) infection is closely associated with depression and development of neuroinflammation. The aim of this study is to explore the relationship between H. pylori, depression, and circulating levels of ghrelin.

Methods: Mice were randomly divided into three groups: healthy control group (gavaged sterile saline and injected with saline, = 8); H. pylori+saline group (gavaged H. pylori and injected with saline, = 8); and H. pylori+rapa group (gavaged H. pylori and injected with rapamycin, = 8). Open field test (OFT), sucrose preference test (SPT), forced swim test (FST), and tail suspension test (TST) were used for anxiety and depressive behavior test. Western blotting was utilized to assess mTOR, p-mTOR, and GSMD expression, and serum ghrelin levels were estimated using ELISA.

Results: In the OFT, the control mice moved more and exhibited a increase in crossing number relative to the H. pylori+saline mice (all < 0.05). Increased quantity of fecal boli can be indicative of increased anxiety and emotionality of the subject animal. H. pylori+saline mice exhibited an increase in fecal boli when compared to control mice and H. pylori+rapa mice ( < 0.05). H. pylori infected mice decreasing the expression of ghrelin. The protein levels of p-mTOR/mTOR in the gastric antrum mTOR signaling activation and low-level ghrelin in H. pylori-infect mice compared to those in control mice (all  <0.001). Compared with single H. pylori infection, mTOR inhibitors increased the ghrelin secretion of H. pylori infection to a certain extent ( < 0.05). The protein levels of GSDMD expression significantly increase in hippocampus of H. pylori-infected mice ( < 0.001). Rapamycin treatment inhibited expression of GSDMD in H. pylori-infected mice ( < 0.05).

Conclusions: H. pylori infection is associated with increased expression of mTOR and decreased circulating levels of ghrelin. Elevated pyroptosis in the brain and anxiety- and depressed-like behaviors occur when ghrelin levels are suppressed.
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http://dx.doi.org/10.1155/2022/2847672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170403PMC
June 2022

Isolation and Complete Sequence of One Novel Marine Bacteriophage PHS21 Infecting Pseudoalteromonas marina.

Curr Microbiol 2022 Jun 7;79(7):213. Epub 2022 Jun 7.

College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, People's Republic of China.

PHS21 against Pseudoalteromonas is isolated from Qingdao offshore seawater. The phage was characterized and identified by morphological examination, stability, whole genome sequencing, and bioinformatics analysis. Morphological analysis of PHS21 by transmission electron microscopy shows that belonged to the Siphoviridae family. PHS21 showed strong tolerance with a wide range of temperatures and pH. One-step growth assay indicates that the latent period is about 48 min and the burst size is approximately 218 PFU/cell (plaque forming unit/cell). Its complete genomic sequence is 35,802-bp long with 50 putative open reading frames. Phage PHS21 and PHS3 displayed a very close evolutionary relationship; however, having different DNA packaging proteins indicates that they may have already evolved distinct ways to package DNA in host cells. This study provides the detailed description and genomic characterization of a novel Pseudoalteromonas phage.
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http://dx.doi.org/10.1007/s00284-022-02886-6DOI Listing
June 2022

Effects of Acupuncture, Moxibustion, Cupping, and Massage on Sports Injuries: A Narrative Review.

Evid Based Complement Alternat Med 2022 28;2022:9467002. Epub 2022 May 28.

Guangzhou Panyu Central Hospital, Guangzhou 511400, China.

With the evolution of society, an increasing number of people have realized the importance of sports on human health. However, participation in sports is a double-edged sword as improperly exercising can lead to injury. Many athletes and patients with sports injuries choose traditional Chinese medicine (TCM) when modern medicine fails to relieve their musculoskeletal symptoms. TCM is a splendid legacy of Chinese civilization whose therapies are effective, economical, and convenient, with some administration by trained patients at home. This review analyzes the literature on the application of acupuncture, moxibustion, massage, and cupping in sports injuries to provide novel ideas for the application of TCM in sports medicine.
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http://dx.doi.org/10.1155/2022/9467002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167071PMC
May 2022

Effects of Noninvasive Brain Stimulation Combined With Antidepressants in Patients With Poststroke Depression: A Systematic Review and Meta-Analysis.

Front Pharmacol 2022 19;13:887115. Epub 2022 May 19.

Central Laboratory, Guangzhou Panyu Central Hospital, Guangzhou, China.

To evaluated the efficacy and safety of noninvasive brain stimulation (NIBS) combined with antidepressants in patients with poststroke depression (PSD). Seven databases were searched to identify randomized controlled trials of NIBS combined with antidepressants in the treatment of PSD based on the international classification of diseases (ICD-10) criteria and exclusion criteria. The retrieval time was from the database establishment to 31 October 2021. Two researchers independently screened the identified studies through the search strategy, extracted their characteristics, and evaluated the quality of the included literature. Cochrane Collaboration's tool was used to assess risk of bias. RevMan 5.3 software was applied for meta-analysis. A total of 34 randomized controlled trials were included, involving 2,711 patients with PSD. Meta-analysis showed that the total effective rate was higher in the combined therapy than the antidepressant alone [odds ratio (OR): 4.33; 95% confidence interval (CI): 3.07 to 6.11; < 0.00001]. The Hamilton depressive scale (HAMD) score was significantly lower in repeated transcranial magnetic stimulation (rTMS) (≤10 Hz) combined with antidepressant than in antidepressant alone [standard mean difference (SMD): -1.44; 95% CI: -1.86 to -1.03; < 0.00001]. No significant difference was seen in rTMS (>10 Hz) combined with antidepressant versus antidepressant alone (SMD: -4.02; 95% CI: -10.43 to 2.39; = 0.22). In addition, combination therapy more strongly improved the modified Barthel index (MBI) scale than antidepressants [mean difference (MD): 8.29; 95% CI: 5.23-11.35; < 0.00001]. Adverse effects were not significantly different between two therapies (OR: 1.33; 95% CI: 0.87 to 2.04; = 0.18). Low-frequency rTMS (≤10 Hz) combined with antidepressants tends to be more effective than antidepressants alone in patients with PSD, and there are no significant adverse effects. In addition, combined therapy may enhance quality of life after stroke. Combination therapy with high-frequency rTMS (>10 Hz) showed no advantage in treating PSD. The transcranial electrical stimulation (TES) combined with antidepressants might be more effective than antidepressants alone, which are needed to confirm by more clinical trials since the.
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http://dx.doi.org/10.3389/fphar.2022.887115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160966PMC
May 2022

Furmonertinib (AST2818) versus gefitinib as first-line therapy for Chinese patients with locally advanced or metastatic EGFR mutation-positive non-small-cell lung cancer (FURLONG): a multicentre, double-blind, randomised phase 3 study.

Lancet Respir Med 2022 Jun 2. Epub 2022 Jun 2.

Shanghai Allist Pharmaceuticals, Shanghai, China.

Background: Furmonertinib (AST2818) is an irreversible, selective, third-generation EGFR tyrosine-kinase inhibitor. We aimed to investigate the efficacy and safety of furmonertinib versus the first-generation EGFR tyrosine-kinase inhibitor gefitinib as first-line treatment in patients with EGFR mutation-positive locally advanced or metastatic non-small-cell lung cancer (NSCLC).

Methods: The FURLONG study is a multicentre, double-blind, randomised, phase 3 study done in 55 hospitals across mainland China. We enrolled patients who were aged 18 years or older and had histologically confirmed, locally advanced or metastatic, stage IIIB, IIIC, or IV unresectable NSCLC with EGFR exon 19 deletions or exon 21 Leu858Arg mutation on tissue biopsy confirmed by a central laboratory. Eligible patients were stratified according to EGFR mutation (exon 19 deletions or exon 21 Leu858Arg) and CNS metastases (with or without) and randomly assigned (1:1) to receive either oral furmonertinib (80 mg/day) or oral gefitinib (250 mg/day) in 21-day cycles until disease progression, the occurrence of intolerable toxicities, withdrawal of consent, or other discontinuation reasons judged by the investigators. Investigators, clinicians, participants, independent review centre (IRC) members, the sponsor, and those analysing the data were all masked to treatment allocation. The primary endpoint was IRC-assessed progression-free survival and, along with safety, was analysed in the full analysis set, which comprised all randomly assigned patients who had received at least one dose of study drug. This study is registered with ClinicalTrials.gov, NCT03787992, and is ongoing for survival follow-up.

Findings: Between May 30, 2019, and Dec 5, 2019, 750 patients were screened, of whom 358 were randomly assigned to receive either furmonertinib and gefitinib-matching placebo (n=178) or gefitinib and furmonertinib-matching placebo (n=180). 178 patients randomly assigned to furmonertinib and 179 patients randomly assigned to gefitinib were treated and were included in the full analysis set. Median follow-up was 21·0 months (IQR 18·0-23·5) in the furmonertinib group and 21·0 months (18·0-23·5) in the gefitinib group. Median IRC-assessed progression-free survival was 20·8 months (95% CI 17·8-23·5) in the furmonertinib group and 11·1 months (9·7-12·5) in the gefitinib group (hazard ratio 0·44, 95% CI 0·34-0·58; p<0·0001). Treatment-related adverse events of a grade 3 or more occurred in 20 (11%) of 178 patients in the furmonertinib group and in 32 (18%) of 179 patients in the gefitinib group. Treatment-related serious adverse events were reported in ten (6%) patients in the furmonertinib group and in 11 (6%) patients in the gefitinib group. Ten (6%) patients in the furmonertinib group and three (2%) patients in the gefitinib group died due to adverse events, which were all judged to be possibly unrelated to study treatment by the investigators.

Interpretation: Furmonertinib showed superior efficacy compared with gefitinib as first-line therapy in Chinese patients with EGFR mutation-positive NSCLC, along with an acceptable safety profile without new signals. Furmonertinib is a new potential treatment option for this population.

Funding: Shanghai Allist Pharmaceuticals and the China National Major Project for New Drug Innovation.

Translation: For the Chinese translation of the abstract see Supplementary Materials section.
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http://dx.doi.org/10.1016/S2213-2600(22)00168-0DOI Listing
June 2022

Effect of Hypertension on Efficacy and Safety of Ticagrelor-Aspirin Versus Clopidogrel-Aspirin in Minor Stroke or Transient Ischemic Attack.

Stroke 2022 Jun 3:101161STROKEAHA122038662. Epub 2022 Jun 3.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, China. (A.W., X.M., H.L., X.X., J.J., J.L., Yilong Wang, X.Z., Z.L., Y.J., L.L., Yongjun Wang).

Background: Hypertension is a risk factor of poor stroke outcomes and associated with antiplatelet resistance. This study aimed to explore the efficacy and safety of ticagrelor-aspirin versus clopidogrel-aspirin in patients with different hypertension status, using randomized trial data from the CHANCE-2 trial (Clopidogrel in High-Risk Patients With Acute Nondisabling Cerebrovascular Events-II).

Methods: A total of 6412 patients with minor stroke or transient ischemic attack who carried loss-of-function alleles were enrolled and randomized to either ticagrelor-aspirin or clopidogrel-aspirin group. Hypertension status were classified into no, newly diagnosed, and previously diagnosed hypertension according to medical history, blood pressure, and antihypertensive medications during hospitalization. The primary efficacy and safety outcomes were stroke recurrence and moderate to severe bleeding risk within 90-day follow-up.

Results: Ticagrelor-aspirin was associated with reduced risk of new stroke in patients without hypertension (32 [4.8%] versus 60 [7.2%]; hazard ratio, 0.55 [95% CI, 0.35-0.86]), but not in those with a newly diagnosed hypertension (20 [5.3%] versus 36 [9.1%]; hazard ratio 0.59 [95% CI, 0.33-1.07]), or those with a previously diagnosed hypertension (139 [7.0%] versus 147 [7.4%]; hazard ratio, 0.93 [95% CI, 0.74-1.18]) compared with clopidogrel-aspirin (=0.04 for interaction). The risk of bleeding for ticagrelor-aspirin was not associated with hypertension status (0.1% versus 0.4%; 0.3% versus 0.5%, 0.4% versus 0.3%, =0.50 for interaction). All the efficacy and safety outcomes between treatments did not differ by blood pressure levels on admission.

Conclusions: In the CHANCE-2 trial, patients without hypertension received a significantly greater benefit from ticagrelor- aspirin than those with previous hypertension after minor stroke or transient ischemic attack, and a similar benefit trend was observed in those with newly diagnosed hypertension.

Registration: URL: http://www.

Clinicaltrials: gov; Unique identifier: NCT04078737.
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http://dx.doi.org/10.1161/STROKEAHA.122.038662DOI Listing
June 2022

Transcriptome Sequencing to Identify Important Genes and lncRNAs Regulating Abdominal Fat Deposition in Ducks.

Animals (Basel) 2022 May 13;12(10). Epub 2022 May 13.

Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.

Abdominal fat deposition is an important trait in meat-producing ducks. F2 generations of 304 Cherry Valley and Runzhou Crested White ducks were studied to identify genes and lncRNAs affecting abdominal fat deposition. RNA sequencing was used to study abdominal fat tissue of four ducks each with high or low abdominal fat rates. In all, 336 upregulated and 297 downregulated mRNAs, and 95 upregulated and 119 downregulated lncRNAs were identified. Target gene prediction of differentially expressed lncRNAs identified 602 genes that were further subjected to Gene Ontology and KEGG pathway analysis. The target genes were enriched in pathways associated with fat synthesis and metabolism and participated in biological processes, including Linoleic acid metabolism, lipid storage, and fat cell differentiation, indicating that these lncRNAs play an important role in abdominal fat deposition. , , , , , , , , , , , , and were involved in the regulation of abdominal fat deposition. , , , , , , , and were regulated by the lncRNAs TCONS_00038080, TCONS_0033547, TCONS_00066773, XR_001190174.3, XR_003492471.1, XR_003493494.1, XR_001192142.3, XR_002405656.2, XR_002401822.2, XR_003497063.1, and so on. This study lays foundations for exploring molecular mechanisms underlying the regulation of abdominal fat deposition in ducks and provides a theoretical basis for breeding high-quality meat-producing ducks.
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http://dx.doi.org/10.3390/ani12101256DOI Listing
May 2022

Recent Advances in Dual-Atom Site Catalysts for Efficient Oxygen and Carbon Dioxide Electrocatalysis.

Small Methods 2022 May 23:e2200408. Epub 2022 May 23.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230029, P. R. China.

Atomically dispersed metal catalysts have been widely used in electrocatalysis because of their outstanding catalytic activity and high atomic utilization efficiency. As an extension of single-atom catalysts (SACs), dual-atom catalysts (DACs) provide new insights for the development of atomic-scale catalysts. Higher metal loading and more flexible active sites endow DACs with improved catalytic performance as well as optimized reaction mechanism model. In this review, DACs are firstly classified according to their configurations and metal sites. Subsequently, the synthetic strategies and characterization techniques of DACs are introduced. Furthermore, the applications of DACs are exemplified in various electrocatalytic reactions, including oxygen reduction reaction, oxygen evolution reaction and carbon dioxide reduction reaction. Finally, the prospects to be expected and challenges to be faced with are discussed.
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http://dx.doi.org/10.1002/smtd.202200408DOI Listing
May 2022

Identification of Angiogenesis-Related Prognostic Biomarkers Associated With Immune Cell Infiltration in Breast Cancer.

Front Cell Dev Biol 2022 6;10:853324. Epub 2022 May 6.

Department of Breast Cancer Center, Chongqing University Cancer Hospital, Chongqing, China.

This study aimed to explore the prognostic value of angiogenesis-related genes (ARGs) and their association with immune cell infiltration (ICI) in breast cancer (BC). Transcriptome data of BC were obtained from the TCGA and GEO databases. Differentially expressed ARGs were identified by the limma package. The identification of key genes and construction of the risk score model were performed by univariate and multivariate Cox regression algorithms. The prognostic value of the risk score was assessed by ROC curves and nomogram. GO, KEGG pathway, and GSEA were used to investigate the biological functions of differentially expressed genes (DEGs), and CIBERSORT, ssGSEA, and xCell algorithms were performed to estimate the ICI in high-risk and low-risk groups. The correlations between prognostic biomarkers and differentially distributed immune cells were assessed. Moreover, a ceRNA regulatory network based on prognostic biomarkers was constructed and visualized by Cytoscape software. A total of 18 differentially expressed ARGs were identified between tumor and adjacent normal tissue samples. TNFSF12, SCG2, COL4A3, and TNNI3 were identified as key prognostic genes by univariate and multivariate Cox regression analyses. The risk score model was further constructed based on the four-gene signature and validated in GSE7390 and GSE88770 datasets. ROC curves and nomogram indicated that the risk score had good accuracy for determining BC patient survival. Biological function analysis showed that DEGs in high- and low-risk groups had a high enrichment in immune-related biological processes and signaling pathways. Moreover, significantly different ICIs were found between high- and low-risk groups, such as memory B cells, CD8 T cells, resting memory CD4 T cells, follicular helper T cells, regulatory T cells, monocytes, M2 macrophages, and neutrophils, and each prognostic biomarker was significantly correlated with one or more immune cell types. The current study identified novel prognostic ARGs and developed a prognostic model for predicting survival in patients with BC. Furthermore, this study indicated that ICI may act as a bond between angiogenesis and BC. These findings enhance our understanding of angiogenesis in BC and provide novel guidance on developing therapeutic targets for BC patients.
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http://dx.doi.org/10.3389/fcell.2022.853324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121305PMC
May 2022

Metal nanoparticles increased the lag period and shaped the microbial community in slurry-electrode microbial electrosynthesis.

Sci Total Environ 2022 Sep 16;838(Pt 1):156008. Epub 2022 May 16.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Concerns about energy crisis and CO emission have motivated the development of microbial electrosynthesis (MES); recent studies have showed the potential of novel slurry-electrode MES. In this study, the effect of nonprecious metal nanoparticles (NPs) on the performance of slurry-electrode MES was systematically evaluated in terms of chemical production, physicochemical properties, electrochemical characterization, and microbial community. Ni and Cu NPs increased the lag period from 6 to 15 days for acetate production, while Mo NPs showed no apparent effect. However, these metal NPs slightly affected the final total acetate production (ca. 10 g L), Faradic efficiency (ca. 50%), net water flux across the anion exchange membrane (ca. 6 mL d), or electrochemical characterization of catholyte. BRH-c20a was enriched as the dominated microbe (>48%), and its relative abundance was largely affected by the addition of metal NPs. This study demonstrates that metal NPs affect the performance of biocathodes, mainly by shaping the microbial community.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156008DOI Listing
September 2022

Rational fabrication of a DNA walking nanomachine on graphene oxide surface for fluorescent bioassay.

Biosens Bioelectron 2022 Sep 10;211:114349. Epub 2022 May 10.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, PR China. Electronic address:

DNA nanomachines, a delicate type of molecular machines, have been a research hotspot in biotechnology and materials. Here a two-dimensional (2D) DNA walking nanomachine with high working efficiency and low cost was easily assembled by using graphene oxide (GO) as the working platform for precisely fluorescent bioassay through the binding of target hepatitis B virus DNA (HBV-DNA) and the driving force of Exonuclease III (Exo III). The presence of HBV-DNA made continuous Exo III digestion of the FAM-modified DNA (FAM-DNA) in double-strand DNA (dsDNA) part in a burnt-bridge mechanism to output a "one-to-more" amplified signal. Accordingly, a 2D DNA walking nanomachine with simple operation and high cost-performance ratio was constructed. The walking speed of nanomachine was found to be regulated by loading DNA density on single sheet of GO. Furthermore, this nanomachine had low background since the dual energy transfer including fluorescence resonance energy transfer (FRET) from FAM to BHQ1 and the long-range resonance energy transfer (LrRET) from FAM to GO, making the biosensing applications highly promising.
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http://dx.doi.org/10.1016/j.bios.2022.114349DOI Listing
September 2022

Association between high-sensitivity C-reactive protein, functional disability, and stroke recurrence in patients with acute ischaemic stroke: A mediation analysis.

EBioMedicine 2022 Jun 13;80:104054. Epub 2022 May 13.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; National Center for Healthcare Quality Management in Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Vascular Neurology, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Chinese Institute for Brain Research, Beijing, China. Electronic address:

Background: Post-stroke inflammation biomarker high-sensitivity C-reactive protein (hsCRP) increases cerebral infarct size and results in functional disability directly, it also contributes to the formation and maturation of atherosclerotic plaques, which increase the risk of stroke recurrence and results in functional disability indirectly. However, no study has quantified how much functional disability was mediated by stroke recurrence.

Methods: Patients with acute ischaemic stroke within 7 days and admitted to 169 hospitals in the Third China National Stroke Registry were analyzed. Blood samples were collected within 24 h of admission. Stroke recurrence and functional disability (defined as a modified Rankin scale score ≥ 2) were assessed via face-to-face interviews at three months. Mediation analysis under the counterfactual framework was performed to examine the potential causal chain in which stroke recurrence may mediate the relationship between hsCRP and functional outcome. Sensitivity analyses were performed across different subgroups and on different scales of hsCRP measurement.

Findings: Of the 7603 analyzed patients (mean [SD] age, 62.3 [11.3] years; 2392 [31.5%] women), the median (interquartile range [IQR]) of NIHSS score was 3.0 (1.0-6.0). The median (IQR) level of hsCRP was 1.73 (0.81-4.38) mg/L. A total of 496 (6.5%) cases of stroke recurrence and 1884 (24.8%) cases of functional disability were observed at the 90-day follow-up. Each SD increase in the concentration of hsCRP was associated with an increased risk of stroke recurrence (adjusted odds ratio [aOR], 1.11; 95% CI, 1.04-1.18) and disability (aOR, 1.14; 95% CI, 1.08-1.20) within 90 days. Of 1884 functionally disabled patients, only 16.0 % (n = 302) of patients experienced stroke recurrence before functional disability. Stroke recurrence during follow-up explained 16.52% (95% CI, 5.79%-27.25%) of the relationship between hsCRP and functional disability. Sensitivity analyses in different subgroups and on different scales of hsCRP measurement showed comparable results.

Interpretation: Stroke recurrence mediates less than 20% of the association between hsCRP and functional disability at 90 days among patients with acute ischaemic stroke. In addition to typical secondary prevention strategies for preventing stroke recurrence, more attention should be paid to novel anti-inflammatory therapy to improve functional outcomes.

Funding: Beijing Natural Science Foundation, the National Key R&D Program of China, the National Natural Science Foundation of China, and the Beijing Municipal Science & Technology Commission.
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http://dx.doi.org/10.1016/j.ebiom.2022.104054DOI Listing
June 2022

Cardiac Segmentation Method Based on Domain Knowledge.

Ultrason Imaging 2022 May 14;44(2-3):105-117. Epub 2022 May 14.

Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.

Echocardiography plays an important role in the clinical diagnosis of cardiovascular diseases. Cardiac function assessment by echocardiography is a crucial process in daily cardiology. However, cardiac segmentation in echocardiography is a challenging task due to shadows and speckle noise. The traditional manual segmentation method is a time-consuming process and limited by inter-observer variability. In this paper, we present a fast and accurate echocardiographic automatic segmentation framework based on Convolutional neural networks (CNN). We propose FAUet, a segmentation method serially integrated U-Net with coordinate attention mechanism and domain feature loss from VGG19 pre-trained on the ImageNet dataset. The coordinate attention mechanism can capture long-range dependencies along one spatial direction and meanwhile preserve precise positional information along the other spatial direction. And the domain feature loss is more concerned with the topology of cardiac structures by exploiting their higher-level features. In this research, we use a two-dimensional echocardiogram (2DE) of 88 patients from two devices, Philips Epiq 7C and Mindray Resona 7T, to segment the left ventricle (LV), interventricular septal (IVS), and posterior left ventricular wall (PLVW). We also draw the gradient weighted class activation mapping (Grad-CAM) to improve the interpretability of the segmentation results. Compared with the traditional U-Net, the proposed segmentation method shows better performance. The mean Dice Score Coefficient (Dice) of LV, IVS, and PLVW of FAUet can achieve 0.932, 0.848, and 0.868, and the average Dice of the three objects can achieve 0.883. Statistical analysis showed that there is no significant difference between the segmentation results of the two devices. The proposed method can realize fast and accurate segmentation of 2DE with a low time cost. Combining coordinate attention module and feature loss with the original U-Net framework can significantly increase the performance of the algorithm.
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http://dx.doi.org/10.1177/01617346221099435DOI Listing
May 2022

Essen score in the prediction of cerebrovascular events compared with cardiovascular events after ischaemic stroke or transient ischaemic attack: a nationwide registry analysis.

J Geriatr Cardiol 2022 Apr;19(4):265-275

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: The Essen risk score improves stratification of patients with acute ischemic stroke by early stroke recurrence. Recent study showed it could also predict myocardial infarction (MI). This study aimed to compare the Essen score's ability to predict cerebrovascular events with compared cardiovascular events.

Methods: We included patients with acute ischaemic stroke or transient ischaemic attack within seven days from the Third China National Stroke Registry. One-year cumulative event rates of combined vascular events (a composite of MI, stroke recurrence or vascular death) and cardiac events (a composite of MI, heart failure or cardiac death) was estimated using the Kaplan-Meier method. The predictive value of the Essen score was assessed with C-statistics. In multivariate Cox regression analyses, we assessed whether Essen score, etiological subtype and imaging parameters were associated with outcomes.

Results: Of 13,012 patients were included, the cumulative one-year event rates were 10.03% for combined vascular events and 0.77% for cardiac events, respectively. Compared with those with an Essen score < 3, patients with an Essen score ≥ 3 were more likely to have a subsequent combined vascular event [hazard ratio (HR) = 1.39, 95% CI: 1.24-1.55] and cardiac events (HR = 2.30, 95% CI: 1.53-3.44). The score tended to be more predictive of the risk of MI (C-statistic = 0.63, 95% CI: 0.55-0.71) and cardiac events (C-statistic = 0.62, 95% CI: 0.56-0.67) than stroke recurrence (C-statistic = 0.55, 95% CI: 0.54-0.57) and combined vascular events (C-statistic = 0.56, 95% CI: 0.54-0.57). In multivariable analysis after adjusted Essen score, patients with multiple acute infarctions or single acute infarctions and large artery atherosclerosis subtype were independently associated with an increased risk of combined vascular events. While the cardioembolism subtype was associated with an increased risk of cardiac events.

Conclusions: The Essen score is potentially more suitable for risk stratification of cardiovascular events than cerebrovascular events. Moreover, future predictive tools should take brain imaging findings and cause of stroke into consideration.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2022.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068593PMC
April 2022

LncRNA CRART16/miR-122-5p/FOS axis promotes angiogenesis of gastric cancer by upregulating VEGFD expression.

Aging (Albany NY) 2022 05 10;14(9):4137-4157. Epub 2022 May 10.

Department of General Surgery, Peking University First Hospital, Beijing 100034, China.

Background: We previously identified a novel lncRNA, CRART16, that could induce cetuximab resistance in colorectal cancer cells. This study explored the relationship of CRART16 expression to gastric cancer progression and the molecular mechanisms involved.

Methods: We evaluated CRART16 expression in gastric cancer tissues and adjacent normal tissues from the TCGA database and our hospital. Besides, we assessed its relationship with the overall survival (OS) of patients with gastric cancer. The effects of CRART16 on gastric cancer angiogenesis were determined by endothelial tube formation assay, spheroid sprouting assay, HUVEC invasion assay, and chick embryo chorioallantoic membrane (CAM) assay. The involvement of the lncRNA CRART16/miR-122-5p/FOS axis was analyzed by western blotting and dual-luciferase reporter assay. The functions of CRART16 were confirmed in xenograft mouse models.

Results: We found that CRART16 was substantially overexpressed in gastric cancer tissues compared with normal tissues, based on the TCGA database and our clinical samples. High expression of CRART16 correlated with more advanced tumor stages and poor prognosis. Overexpression of CRART16 in gastric cancer cells promoted proliferation, colony formation, angiogenesis, and bevacizumab resistance , and it promoted tumor growth and angiogenesis , and vice versa. CRART16 was found to downregulate miR-122-5p by acting as a sponge, upregulating the target oncogene FOS. Afterward, the increased FOS expression led to the upregulation of VEGFD.

Conclusion: Our findings demonstrate that CRART16 promotes angiogenesis and , and CRART16 is a prognostic marker and therapeutic target in gastric cancer.
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http://dx.doi.org/10.18632/aging.204078DOI Listing
May 2022

Roughness Effect of Cu on Electrocatalytic CO Reduction towards C H.

Chem Asian J 2022 May 10:e202200380. Epub 2022 May 10.

College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, P. R. China.

Electrochemical reduction of CO to produce valuable multi-carbon products is a promising avenue for promoting CO conversion and achieving renewable energy storage, and it has also attracted considerable attention recently. However, the synthesis of Cu electrode with a controllable electrochemical active surface area (ECSA) to understand its role in CO reduction to C H remains challenging. Herein, a series of Cu electrodes with different ECSA is synthesized through a simple oxidation-reduction approach. We reveal that the improved selectivity of C H is proportional to the ECSA of Cu in the low ECSA range, and a further increase in ECSA has a negligible effect on its selectivity. The enlarged surface area could strengthen the local pH effect near the surface of Cu electrode and suppress the generation of C products as well as H . The study provides a feasible strategy to rationally design electrocatalysts with high electrochemical CO reduction performances.
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http://dx.doi.org/10.1002/asia.202200380DOI Listing
May 2022

Intertwined Relation between the Endoplasmic Reticulum and Mitochondria in Ischemic Stroke.

Oxid Med Cell Longev 2022 28;2022:3335887. Epub 2022 Apr 28.

Department of Neurosurgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

In ischemic stroke (IS), accumulation of the misfolded proteins in the endoplasmic reticulum (ER) and mitochondria-induced oxidative stress (OS) has been identified as the indispensable inducers of secondary brain injury. With the increasing recognition of an association between ER stress and OS with ischemic stroke and with the improved understanding of the underlying molecular mechanism, novel targets for drug therapy and new strategies for therapeutic interventions are surfacing. This review discusses the molecular mechanism underlying ER stress and OS response as both causes and consequences of ischemic stroke. We also summarize the latest advances in understanding the importance of ER stress and OS in the pathogenesis of ischemic stroke and discuss potential strategies and clinical trials explicitly aiming to restore mitochondria and ER dynamics after IS.
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http://dx.doi.org/10.1155/2022/3335887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072026PMC
May 2022

Community assembly and co-occurrence network complexity of pelagic ciliates in response to environmental heterogeneity affected by sea ice melting in the Ross Sea, Antarctica.

Sci Total Environ 2022 Aug 4;836:155695. Epub 2022 May 4.

College of Marine Life Science & Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao 266003, China. Electronic address:

In the Southern Ocean, the living environment of organisms has changed due to the dramatic increase in melting sea ice and the loss of glaciers, which have consequently caused substantial changes in biodiversity. Samples of pelagic ciliates from 13 sites were collected as bioindicators to demonstrate the relationship between spatial distribution patterns and environmental heterogeneity affected by sea ice melting and to reveal the community assembly mechanisms in the Ross Sea. Univariate analyses and multivariate analyses were effective tools demonstrating clear spatial patterns and providing a sufficient explanation to interpret strong correlations between pelagic ciliate communities and environmental variations, especially the distribution pattern of nutrients and Chl a. Moreover, environmental heterogeneity might affect the co-occurrence network complexity of ciliate communities. Furthermore, our results also indicated that stochastic processes play a significant role in the community assembly of pelagic ciliates. This study examined the controlling mechanisms of environmental heterogeneity affected by sea ice melting on pelagic ciliate communities and provided explanations for the community assembly of pelagic ciliates in polar marine ecosystems.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155695DOI Listing
August 2022

Prevalence and In-hospital outcomes of diabetes among acute ischemic stroke patients in china: results from the Chinese Stroke Center Alliance.

J Neurol 2022 May 5. Epub 2022 May 5.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, No 119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.

Background: Patients with ischemic stroke and diabetes are classified as extreme risk for secondary prevention, with much attention and specific management. However, the up-to-date information regarding the burden of diabetes in acute ischemic stroke (AIS) patients is lacking in China, and evidence for an association between diabetes and in-hospital outcomes after AIS remains controversial.

Methods: This quality improvement study was conducted at 1,476 participating hospitals in the Chinese Stroke Center Alliance between 2015 and 2019. Prevalence of diabetes was evaluated in the overall study population and different subgroups. The association between diabetes and in-hospital outcomes in AIS patients was analyzed by using multivariable logistic regression analysis and propensity score-matched analysis.

Results: Of 838,229 patients with AIS, 286,252 (34.2%) had diabetes/possible diabetes. The prevalence of diabetes/possible diabetes was higher in women than in men (37.6% versus 32.1%). Patients with diabetes/possible diabetes had higher rates of adverse in-hospital outcomes than those without. Multivariable analysis revealed a significant association between diabetes/possible diabetes and adverse in-hospital outcomes (all-cause mortality: odds ratio [OR], 1.30 [95% confidence interval [CI], 1.23-1.38]; major adverse cardiovascular events (MACEs): OR, 1.08 [95% CI, 1.06-1.10]) in AIS patients. The excess risk of in-hospital outcomes still remained in AIS patients with diabetes/possible diabetes after propensity score-matching analysis (all-cause mortality: OR, 1.26 [95% CI, 1.17-1.35]; MACEs: OR, 1.07 [95% CI, 1.05-1.10]).

Conclusion: Diabetes was highly prevalent among AIS patients in China and associated with worse in-hospital outcomes. Greater efforts to increase targeted approach to secondary prevention treatments of diabetes in AIS patients are warranted.
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http://dx.doi.org/10.1007/s00415-022-11112-zDOI Listing
May 2022
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