Publications by authors named "Yong Huang"

2,043 Publications

  • Page 1 of 1

Effect of monosultap on notochord development in zebrafish (Danio rerio) embryos.

Toxicology 2022 Aug 3:153276. Epub 2022 Aug 3.

Ganzhou Key Laboratory for Drug Screening and Discovery, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, 341000 Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Jinggangshan University, Ji'an, 343009, Jiangxi, China. Electronic address:

Monosultap (Mon) is a broad-spectrum insecticide used in agricultural production to control stem borers in rice fields. Currently, little evidence shows how Mon affects notochord development in zebrafish (Danio rerio). In our study, zebrafish embryos were exposed to 0.25, 0.5, and 0.75mg/L Mon to determine the effects of different concentrations of Mon on notochord development. Mon exposure reduced the body length, decreased the heart rate and hatchability, and induced notochord deformity in zebrafish. The effects of Mon exposure on the internal organization of the notochord and the structural abnormalities were determined based on histological staining of paraffinized tissue sections. Quantitative polymerase chain reaction (qPCR) and in situ hybridization findings revealed that the expression levels of genes related to notochord development (shha, col2a, and ptch2) showed an increasing trend in a concentration-dependent manner. An abnormal increase of apoptosis and cell proliferation in some parts of the notochord suggested that Mon exposure could cause developmental abnormality of the notochord. This study revealed the toxicity of Mon in notochord development. Our findings provide information in assessing the risk of Mon to the ecological environment and human health.
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http://dx.doi.org/10.1016/j.tox.2022.153276DOI Listing
August 2022

Multi-Omics Analysis of the Tumor Microenvironment in Liver Metastasis of Colorectal Cancer Identified FJX1 as a Novel Biomarker.

Front Genet 2022 19;13:960954. Epub 2022 Jul 19.

Department of Gastroenterology, Lianshui People's Hospital Affiliated to Kangda College of Nanjing Medical University, Huai'an, China.

Colorectal cancer incidence and mortality have increased in recent years, with more than half of patients who died of colorectal cancer developing liver metastases. Consequently, colorectal cancer liver metastasis is the focus of clinical treatment, as well as being the most difficult. The primary target genes related to colorectal cancer liver metastasis were via bioinformatics analysis. First, five prognosis-related genes, CTAG1A, CSTL1, FJX1, IER5L, and KLHL35, were identified through screening, and the prognosis of the CSTL1, FJX1, IER5L, and KLHL35 high expression group was considerably poorer than that of the low expression group. Furthermore, the clinical correlation analysis revealed that in distinct pathological stages T, N, and M, the mRNA expression levels of CSTL1, IER5L, and KLHL35 were higher than in normal tissues. Finally, a correlation study of the above genes and clinical manifestations revealed that FJX1 was strongly linked to colorectal cancer liver metastasis. FJX1 is thought to affect chromogenic modification enzymes, the Notch signaling system, cell senescence, and other signaling pathways, according to KEGG enrichment analysis. FJX1 may be a critical target in colorectal cancer metastasis, and thus has the potential as a new biomarker to predict and treat colorectal cancer liver metastases.
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http://dx.doi.org/10.3389/fgene.2022.960954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343787PMC
July 2022

An NHC-catalyzed [3+2] cyclization of β-disubstituted enals with benzoyl cyanides.

Chem Commun (Camb) 2022 Aug 4. Epub 2022 Aug 4.

Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China.

The NHC-catalyzed asymmetric [3+2] cyclization of benzoyl cyanides to homoenolate generated from enals was reported. This methodology leads to the efficient construction of a series of chiral cyclic compounds bearing vicinal quaternary stereocenters under mild reaction conditions. Additionally, the representative large-scale and derivatization reactions of the chiral cyclic products reveal the potential synthetic utility of this protocol.
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http://dx.doi.org/10.1039/d2cc04025eDOI Listing
August 2022

Anti-müllerian hormone as a predictor for live birth among women undergoing IVF/ICSI in different age groups: an update of systematic review and meta-analysis.

Arch Gynecol Obstet 2022 Jul 30. Epub 2022 Jul 30.

Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095, Jiefang Avenue, Wuhan, 430030, Hubei, People's Republic of China.

Purpose: To update the evidence of anti-müllerian hormone (AMH) as predictive factors for live birth outcome in women undergoing assisted conception and discover the modulating effect of age.

Methods: PubMed, Embase, Medline, and Web of Science were searched for studies published until June 2021. We included studies that measured serum AMH levels and reported the subsequent live birth outcomes. Random effects models and hierarchical summary receiver operating characteristics (HSROC) models were used. The QUADAS-2 checklist was employed to assess the quality of the included studies.

Results: We included 27 studies (27,029 women) investigating the relationship between AMH and live birth outcome after assisted conception. The diagnostic odds ratios (DOR) from random effects models were ruled out due to high heterogeneity. Our findings suggested that AMH was associated with live birth. The DOR was 2.21 (95% CI 1.89-2.59), and 2.49 (95% CI 1.26-4.91) for studies on women with unspecified ovarian reserve and women with low ovarian reserve, respectively. The DOR of those with advanced ages was 2.50 (95% CI 1.87-2.60). For younger women, the DOR was 1.41 (95% CI 0.99-2.02). HSROCs showed that AMH had no predictive ability towards live birth in women with diminished ovarian reserve or younger age. Exclusion of Chinese cohorts lowered the heterogeneity.

Conclusions: This study revealed that AMH had better prediction for live birth in advanced-age women. AMH may have implicative predictive value for assisted conception counseling of couples of advanced ages.
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http://dx.doi.org/10.1007/s00404-022-06683-1DOI Listing
July 2022

The effects of Baduanjin and yoga exercise programs on physical and mental health in patients with Multiple Sclerosis: A randomized controlled trial.

Complement Ther Med 2022 Jul 26;70:102862. Epub 2022 Jul 26.

Department of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou, China; Clinical Medical College, Yangzhou University, Yangzhou, China. Electronic address:

Objective: To discuss whether Baduanjin and yoga exercise interventions improve motor function, posture control, and relieving fatigue and depression in MS patients. And to explore whether practicing Baduanjin benefits MS patients more than yoga.

Design: A prospective, randomized, controlled, three-arm trial comparing BDJ (n = 30), yoga (n = 30) and control group (n = 20).

Setting: Jiangsu Provincial Corps Hospital.

Intervention: Eligible participants were randomized to a 24-week Baduanjin or yoga intervention, or a usual activity control group. Balance, posture control and trunk movement were measured with the Berg Balance Scale (BBS) and Trunk Impairment Scale (TIS). Fatigue was measured using the Fatigue Severity Scale (FSS) and depressive symptoms via the Zung Self-Rating Depression Scale (SDS).

Results: For BBS and TIS, there were significant changes pre- to post- exercise in two exercise groups (P < 0.05), with greater increases in the Baduanjin exercise group (BDJ group). For the FSS, there were significant changes pre- to post- exercise in both the BDJ (P = 0.0292) and yoga groups (P = 0.0150). For the SDS, the pre- and post-exercise difference of the BDJ group was larger than the yoga group (P < 0.0001). On the other hand, we could not find any changes of the BBS, TIS, FSS, and SDS scores in the control group (p > 0.05).

Conclusion: The results suggest that practicing Baduanjin was more effective than yoga and that it is suitable for the MS patients.
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http://dx.doi.org/10.1016/j.ctim.2022.102862DOI Listing
July 2022

Artemisinin relieves osteoarthritis by activating mitochondrial autophagy through reducing TNFSF11 expression and inhibiting PI3K/AKT/mTOR signaling in cartilage.

Cell Mol Biol Lett 2022 Jul 28;27(1):62. Epub 2022 Jul 28.

Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213000, China.

Osteoarthritis (OA) is a widespread chronic degenerative joint disease characterized by the degeneration of articular cartilage or inflamed joints. Our findings indicated that treatment with artemisinin (AT) downregulates the protein levels of MMP3, MMP13, and ADAMTS5, which are cartilage degradation-related proteins in OA, and inhibits the expression of inflammatory factors in interleukin-1β (IL-1β)-stimulated chondrocytes. However, the mechanism of the role of AT in OA remains unclear. Here, we performed gene sequencing and bioinformatics analysis in control, OA, and OA + AT groups to demonstrate that several mRNA candidates were enriched in the PI3K/AKT/mTOR signaling pathway, and TNFSF11 was significantly downregulated after AT treatment. TNFSF11 was downregulated in the OA + AT group, whereas it was upregulated in rat OA tissues and OA chondrocytes. Therefore, we confirmed that TNFSF11 was the target gene of AT. In addition, our study revealed that AT relieved cartilage degradation and defection by activating mitochondrial autophagy via inhibiting the PI3K/AKT/mTOR signaling pathway in IL-1β-induced chondrocytes. Furthermore, an OA model was established in rats with medial meniscus destabilization. Injecting AT into the knee joints of OA rat alleviated surgical resection-induced cartilage destruction. Thus, these findings revealed that AT relieves OA by activating mitochondrial autophagy by reducing TNFSF11 expression and inhibiting PI3K/AKT/mTOR signaling.
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http://dx.doi.org/10.1186/s11658-022-00365-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331798PMC
July 2022

Transcriptomic Analysis of Sitophilus zeamais in Response to Limonene Fumigation.

Pest Manag Sci 2022 Jul 28. Epub 2022 Jul 28.

Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.

Background: Frequent application of chemical fumigants has contributed to the development of resistance in stored-product pests. Essential oils provide a novel and environmentally friendly alternative to conventional chemical pesticides. In this work, the fumigant activity of Taxodium 'zhongshansha' essential oil (TZEO) and main active components against Sitophilus zeamais were evaluated. In addition, the molecular mechanisms mediating the fumigant activity of limonene were assessed.

Results: TZEO showed strong fumigant activity against S. zeamais, with a 50% lethal concentration (LC ) of 22.90 μL/L air in 24 h. The main components of TZEO were identified using gas chromatography-mass spectrometry, the main active ingredient (limonene) showed an LC of 9.93 μL/L air in 24 h which had a serious dose-time-effect. The LC value of the positive control (aluminum phosphide) was 1.91 μL/L. In total, 3,982 upregulated and 3,067 downregulated genes were sequenced in limonene-fumigated S. zeamais, the genes related to metabolic detoxification were significantly enriched. The mortality rate of 7 day-old S. zeamai adult mediated with knockdown of SzCYP6MS5 and SzCYP6MS6 raised up to 65.67% and 67.65% after fumigated with limonene in 24 h, respectively. The results showed that SzCYP6MS5 and SzCYP6MS6 are closely involved to the detoxification of limonene.

Conclusion: In this study, candidate genes affected by limonene treatment in S. zeamais were identified. These findings provided insights into the systemic metabolic response of S. zeamais to limonene and established a basis for the development of limonene as a botanical pesticide for the control of stored-product pests. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ps.7097DOI Listing
July 2022

Deformed Mediated Larval Incisor Lobe Development Causes Differing Feeding Behavior between Oriental Armyworm and Fall Armyworm.

Insects 2022 Jun 29;13(7). Epub 2022 Jun 29.

Anhui Province Key Laboratory of Crop Integrated Pest Management, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China.

Mandibular incisor lobes are important for insect feeding behavior, living habits and niche. However, the molecular regulation of insect incisor lobe development remains unknown. In this study, we found that two maize pests, oriental armyworm and fall armyworm , have different feeding patterns in maize, which are closely associated with the different development patterns of their incisor lobes. Different from first to sixth instar , which feed on leaf tissues and whorls with sharp incisor lobes, older instars of feed from leaf margins with no incisor lobes. Hox gene () is important for head appendages, but its function in incisor lobe development is not clear. Here, were identified from two armyworm species, and both were expressed highly in heads and eggs. Interestingly, the expression levels of were relatively high in larval mandibles and decreased dramatically from fourth-instar mandibles in . Knockdown of resulted in malformed mandibles with no incisor lobe in , making the larvae unable to perform window-feeding. However, RNAi of did not affect the mandibles and window-feeding pattern of , indicating the different roles of in these two species. Moreover, the mortality of new first instar increased after feeding ds but did not for feeding ds. These findings revealed that mediated the larval mandibular incisor lobe morphology, affecting its feeding pattern in , broadening the knowledge of functions in insect mandibles and feeding behavior.
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http://dx.doi.org/10.3390/insects13070594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320430PMC
June 2022

Generation of Gal-enhanced bifunctional tumor vaccine.

Acta Pharm Sin B 2022 Jul 9;12(7):3177-3186. Epub 2022 Mar 9.

National Center for International Research of Biotargeting Theranostics, Guangxi Key Laboratory of Biotargeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Biotargeting Theranostics, Guangxi Medical University, Nanning 530021, China.

Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis and high mortality. In this study, we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant MHCC97H cells expressing porcine -1,3-galactose epitopes (Gal) and endorphin extracellular domains (END) with dendritic cells (DCs) from healthy volunteers. END/Gal-MHCC97H/DC fusion cells induced cytotoxic T lymphocytes (CTLs) and secretion of interferon-gamma (IFN-). CTLs targeted cells expressing Gal and END and tumor angiogenesis. The fused cell vaccine can effectively inhibit tumor growth and prolong the survival time of human hepatoma mice, indicating the high clinical potential of this new cell based vaccine.
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http://dx.doi.org/10.1016/j.apsb.2022.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293690PMC
July 2022

Targeted Anti-Hepatocellular Carcinoma Research of Targeted Peptides Combined with Drug-Loaded Cell-Derived Microparticles.

J Biomed Nanotechnol 2022 Apr;18(4):1009-1018

National Center for International Research of Biotargeting Theranostics, Guangxi Key Laboratory of Biotargeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Biotargeting Theranostics, Guangxi Medical University, Nanning, Guangxi, 530021, China.

To conduct an anti-tumor research by using targeted drug-loaded cell-derived microparticles to target the tumor microenvironment and enhance NK cell killing function. In this experiment, we obtained HepG2 tumor cell-derived microparticles by physical extrusion, high speed centrifugation and filtration, modified the hepatocellular carcinoma targeting peptide SP94 on the surface of microparticles and encapsulated the TGF- inhibitor SB505124. Finally we validated and analyzed whether the new drug delivery system can target to tumor site and enhance the anti-tumor function of NK cells. This type of novel targeted cell-derived microparticles drug delivery system will provide a novel idea for tumor immunotherapy.
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http://dx.doi.org/10.1166/jbn.2022.3311DOI Listing
April 2022

[Pharmacokinetics and tissue distribution of four components from root bark of Caesalpinia decapetala in rats by UPLC-MS/MS].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(13):3629-3636

State Key Laboratory of Functions and Applications of Medicinal Plants, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University Guiyang 550004, China.

To identify the pharmacodynamic material basis of root bark of Caesalpinia decapetala extract and clarify the dynamic changes and distribution characteristics of the compounds in vivo.UPLC-MS/MS was used for simultaneous determination of 3-deoxysappanchalcone, isoliquiritigenin, protosappanin B, and protosappanin B-10-O-β-D-glucoside in plasma, heart, liver, spleen, lung, kidney, stomach and duodenum of rats, to further study the pharmacokinetics and tissue distribution of root bark of C.decapetala extract in rats.Statistical analysis of obtained data demonstrated that the established analytical methods of the four components in biological matrix met the requirements of biological sample determination.The pharmacokinetic parameters showed that the t_(1/2 z), T_(max), C_(max), AUC_(0-t), MRT_(0-t), and CL_(z/F) of each component were 4.57-13.47 h, 0.22-0.51 h, 27.60-6 418.38 μg·L~(-1), 112.45-11 824.25 h·μg·L~(-1), 3.89-9.01 h, and 9.85-96.87 L·h~(-1)·kg~(-1), respectively.The results of tissue distribution revealed that at different time points, the components were widely but unevenly distributed in the body.Specifically, they were more distributed in the stomach and duodenum, followed by liver, spleen, lung, and kidney, and the least distribution was observed in the heart.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220215.203DOI Listing
July 2022

Lipopeptide surfactin ameliorates the cell uptake of platensimycin and enhances its therapeutic effect on treatment of MRSA skin infection.

J Antimicrob Chemother 2022 Jul 15. Epub 2022 Jul 15.

Xiangya International Academy of Translational Medicine at Central South University, Changsha, Hunan 410013, China.

Objectives: The rapid development of drug-resistant bacteria, especially MRSA, poses severe threats to global public health. Adoption of antibiotic adjuvants has proved to be one of the efficient ways to solve such a crisis. Platensimycin and surfactin were comprehensively studied to combat prevalent MRSA skin infection.

Methods: MICs of platensimycin, surfactin or their combinations were determined by resazurin assay, while the corresponding MBCs were determined by chequerboard assay. Growth inhibition curves and biofilm inhibition were determined by OD measurements. Membrane permeability analysis was conducted by propidium iodide staining, and morphological characterizations were performed by scanning electron microscopy. Finally, the therapeutic effects on MRSA skin infections were evaluated in scald-model mice.

Results: The in vitro assays indicated that surfactin could significantly improve the antibacterial performance of platensimycin against MRSA, especially the bactericidal activity. Subsequent mechanistic studies revealed that surfactin not only interfered with the biofilm formation of MRSA, but also disturbed their cell membranes to enhance membrane permeability, and therefore synergistically ameliorated MRSA cellular uptake of platensimycin. Further in vivo assessment validated the synergistic effect of surfactin on platensimycin and the resultant enhancement of therapeutical efficacy in MRSA skin-infected mice.

Conclusions: The combination of effective and biosafe surfactin and platensimycin could be a promising and efficient treatment for MRSA skin infection, which could provide a feasible solution to combat the major global health threats caused by MRSA.
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http://dx.doi.org/10.1093/jac/dkac228DOI Listing
July 2022

Cell metabolomics study on synergistic anti-hepatocellular carcinoma effect of Aidi injection combined with doxorubicin.

Biomed Chromatogr 2022 Jul 18:e5451. Epub 2022 Jul 18.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, China.

Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and the second most common cause of cancer deaths. This study aimed to explore the inhibitory effect and mechanism of Aidi injection (ADI) combined with doxorubicin (DOX) in HCC treatment. The drug concentrations in the combined therapy was determined by investigating the effect of various concentrations of ADI and DOX on the viability of H22 cells. The combination index was also calculated. A metabolomic strategy based on a ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) platform was established to analyze the metabolites. As a result, the combination index values were less than 1, indicating that the combination of ADI and DOX exerted a synergistic effect on HCC treatment. The combination of 40‰ ADI and 1 μmol/l DOX had the strongest inhibitory effect and was used for subsequent metabolomic analysis. A total of 19 metabolic markers were obtained in metabolomic analysis, including amino acids (l-glutamic acid, l-arginine and l-tyrosine), organic acids (succinic acid and citric acid), adenosine and hypoxanthine. Compared with the treatment using DOX or ADI alone, the combined therapy further regulated the levels of metabolic markers in HCC, which may be the reason for the synergistic effect. Seven metabolic pathways were significantly enriched, including phenylalanine, tyrosine and tryptophan biosynthesis, d-glutamine and d-glutamate metabolism, alanine, aspartate and glutamate metabolism, phenylalanine metabolism, arginine biosynthesis, the tricarboxylic acid cycle and purine metabolism. These findings demonstrated that ADI combined with DOX can effectively inhibit the viability of H22 cells, which may exert a synergistic antitumor effect by balancing the metabolism of amino acids and energy-related substances.
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http://dx.doi.org/10.1002/bmc.5451DOI Listing
July 2022

Metagenomics reveals the metabolism of polyphosphate-accumulating organisms in biofilm sequencing batch reactor: A new model.

Bioresour Technol 2022 Jul 12;360:127603. Epub 2022 Jul 12.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

This study assessed the impact of the operating conditions of the biofilm sequencing batch reactor (BSBR) on the community structure and the growth/metabolic pathways of its polyphosphate-accumulating organisms (PAOs). There are significant difference with reference to the enhanced biological phosphorus removal (EBPR) process. The leading PAOs in BSBR generally are capable of high affinity acetate metabolism, gluconeogenesis, and low affinity phosphate transport, and have various carbon source supplementation pathways to ensure the efficient circulation of energy and reducing power. A new model of the metabolic mechanism of PAOs in the BSBR was formulated, which features low glycogen metabolism with simultaneous gluconeogenesis and glycogenolysis and differs significantly from the classic mechanism based on Candidatus_Accumulibacter and Tetrasphaera. The findings will assist the efficient recovery of low concentration phosphate in municipal wastewater.
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http://dx.doi.org/10.1016/j.biortech.2022.127603DOI Listing
July 2022

Pharmacokinetics Study of Jin-Gu-Lian Prescription and Its Core Drug Pair ( (Oliv.) Rehd. et W and (Lour.) Harms) by UPLC-MS/MS.

Molecules 2022 Jun 23;27(13). Epub 2022 Jun 23.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.

Jin-Gu-Lian (JGL) is traditionally used by Miao for the treatment of rheumatism arthralgia. At the same time, the combination of (Oliv.) Rehd. et W (SC) and (Lour.) Harms (AC), the core drug pair (CDP) in the formula of JGL, is used at high frequencies in many Miao medicine prescriptions for rheumatic diseases. However, previous research lacks the pharmacokinetic study of JGL, and study on the compatibility of its CDP with other medicinal herbs in the formula is needed. This study aims to establish a simple, rapid, and sensitive Ultra Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous determination of four main bioactive components of JGL in rat plasma, including Salidroside (Sal), Anabasine (Ana), Chlorogenic Acid (CA), and Protocatechuic Acid (PCA), and compare the pharmacokinetic properties of two groups of rats after being orally administrated with JGL and its CDP extracts, respectively. The results showed that area under the plasma concentration-time curve (AUC), mean retention time (MRT), and clearance rate (CL), of Sal, Ana, CA and PCA in the two groups of rats were changed in different degrees. The CDP combined with other drugs could significantly increase the absorption of Sal and Ana, prolong its retention time in vivo, and may accelerate the absorption rate of CA and PCA. This indicated that the combination of CDP and other herbs may affect the pharmacokinetics process of active components in vivo, increase the exposure and bioavailability of compounds in the JGL group, and prolong the retention time, which may be the reason why JGL has a better inhibitory effect on inflammatory cytokines, providing a viable orientation for the compatibility investigation of herb medicines.
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http://dx.doi.org/10.3390/molecules27134025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268445PMC
June 2022

P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.

Cancers (Basel) 2022 Jul 1;14(13). Epub 2022 Jul 1.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215000, China.

Lung cancer is associated with the greatest number of cancer-related deaths worldwide. Lung adenocarcinoma (LUAD) accounts for 85% of all cases of lung cancer. Despite recent advances in treatment, the 5-year survival rate remains less than 15%. Thus, the diagnostic and therapeutic role of LUAD remain to be further studied. The prolyl 3-hydroxylase family member 4 (P3H4) is involved in various cancers, but little is known about its role in LUAD. Our study demonstrated that the P3H4 gene was upregulated in LUAD. Clinically, the expression of P3H4 was positively correlated with an advanced TNM stage and shorter survival. Functionally, P3H4 plays a significant role in the metastasis and proliferation of LUAD both in vitro and in vivo. Mechanistically, P3H4 might interact with EGFR to regulate the metabolic substances. Our study indicated that P3H4 is a critical gene in the malignant progression of LUAD and represents a potential biomarker and therapeutic target.
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http://dx.doi.org/10.3390/cancers14133243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264976PMC
July 2022

A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?

Front Bioeng Biotechnol 2022 20;10:912073. Epub 2022 Jun 20.

J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States.

The gap between and assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed . A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between and assays and guide how vascular biologists and physiologists might think about the microcirculation.
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http://dx.doi.org/10.3389/fbioe.2022.912073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252339PMC
June 2022

Proton Self-Enhanced Hydroxyl-Enriched Cerium Oxide for Effective Arsenic Extraction from Strongly Acidic Wastewater.

Environ Sci Technol 2022 07 6;56(14):10412-10422. Epub 2022 Jul 6.

National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, P. R. China.

Acid recycling and arsenic recovery from strongly acidic wastewater are goals of the metallurgical industry to reduce carbon emissions. In this study, arsenic was recovered using a hydroxyl-enriched CeO adsorbent, and the adsorption mechanism in a strongly acidic solution was investigated. The adsorption capacities of 88.59 mg/g for As(III) and 126.211 mg/g for As(V) at pH 1.0 are the highest reported values to date. It is revealed that the hydroxyl groups on the CeO surface can buffer hydrogen ions, and the isoelectric point of the material can be reduced to pH 1.52. The binding energy of arsenic is -1.25 eV for the hydroxyl-enriched CeO and -2.24 eV for CeO without hydroxyl groups. Additionally, the protonated hydroxyl groups reduce the oxidation energy of As(III) and promote the adsorption of arsenic by forming new active sites in the strongly acidic solution. Nearly 98.11% of arsenic (initial concentration is 886.8 mg/L) is removed within 24 h without pH adjustment, indicating the feasibility of hydroxyl-enriched CeO for recovering arsenic and acid. This work investigated the adsorption and proton-enhanced oxidation mechanism of arsenic by hydroxyl-enriched CeO in strongly acidic wastewater.
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http://dx.doi.org/10.1021/acs.est.2c02675DOI Listing
July 2022

Therapeutic Potential of Triptolide in Treating Bone-Related Disorders.

Front Pharmacol 2022 15;13:905576. Epub 2022 Jun 15.

Department of Spinal Surgery, Hubei Provincial Hospital of TCM, Wuhan, China.

Triptolide, a diterpene triepoxide, is a pharmacologically active compound isolated from a Chinese medicinal herb Hook F (TwHF). Triptolide has attracted considerable attention in recent times due to its multiple biological and pharmaceutical activities, with an emphasis on therapeutic importance in the treatment of diverse disorders. With essential medicinal implications, TwHF's extracts have been used as anti-inflammatory, antiproliferative, antioxidative, and immunosuppressive agents for centuries, with continuous and relevant modifications to date to enhance its utility in several diseases and pathophysiology. Here, in this review, we accentuate the studies, highlighting the effects of triptolide on treating bone-related disorders, both inflammatory and cancerous, particularly osteosarcoma, and their manifestations. Based on this review, future avenues could be estimated for potential research strategies, molecular mechanisms, and outcomes that might contribute toward reinforcing new dimensions in the clinical application of triptolide in treating bone-related disorders.
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http://dx.doi.org/10.3389/fphar.2022.905576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240268PMC
June 2022

Transforaminal Endoscopic Lumbar Discectomy for Lumbosacral Junction Adolescent Lumbar Disc Herniation with High Iliac Crests.

Orthop Surg 2022 Jun 29. Epub 2022 Jun 29.

Department of Spine Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

Objective: To investigate the clinical results of transforaminal endoscopic lumbar discectomy for lumbosacral junction adolescent lumbar disc herniation with high iliac crest.

Methods: From February 2014 to September 2020, a retrospective analysis of 96 patients less than 21 years old with intervertebral disc herniation was carried out. We enrolled 44 patients diagnosed with lumbosacral junction disc herniation with high iliac crest who required transforaminal endoscopic lumbar discectomy. Pain in the back and the lower extremity was scored on Numeric Rating Scales (NRS) scores. Patient outcomes were graded as excellent, good, fair, and poor using modified MacNab criteria. The NRS scores before and after the operation were compared using the Wilcoxon two-sample test.

Results: There were 30 male patients and 14 females. One patient underwent repeat surgery for an intervertebral disc pseudocyst. The NRS scores decreased significantly in both early and late follow-up evaluations (p < 0.05). At the last follow-up, 42 patients (95.45%) had an excellent outcome, one patient (2.27%) had a good outcome, and one patient (2.27%) had a fair outcome. The overall success rate was 97.7%.

Conclusion: This study's data suggest that targeted individualized foraminoplasty can effectively overcome the lumbosacral anatomical obstacles, and transforaminal endoscopic lumbar discectomy is an effective and valid option for lumbosacral junction adolescent lumbar disc herniation with high iliac crest.
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http://dx.doi.org/10.1111/os.13355DOI Listing
June 2022

SEMA6D, Negatively Regulated by miR-7, Contributes to C28/I2 chondrocyte's Catabolic and Anabolic Activities via p38 Signaling Pathway.

Oxid Med Cell Longev 2022 16;2022:9674221. Epub 2022 Jun 16.

Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou 213000, China.

MiR-7 has been recognized as an osteoarthritis (OA-)-promoting factor, but the specific downstream pathway of miR-7 still remains unknown. Further investigation of the molecular regulatory mechanism of miR-7 might help develop a novel therapeutic method for OA. In this study, we revealed that Semaphorin 6D (SEMA6D) was a direct target gene of miR-7 and presented a negative regulatory relation with SEMA6D and . SEMA6D could improve OA in rat OA models, as indicated by H&E and Safranin O-Fast green staining, and also CT analysis. Further evaluation of SEMA6D suggested that SEMA6D promotes the anabolism and reduces the catabolism of C28/I2 chondrocytes via inhibiting the activation of the p38 pathway. The present research illustrated that SEMA6D is a negatively regulatory factor of miR-7 and a pivotal mediator of catabolism and anabolism in C28/I2 chondrocytes. SEMA6D exerts its function via inhibiting the activation of the p38 pathway.
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http://dx.doi.org/10.1155/2022/9674221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225841PMC
June 2022

Solution-Processed Perovskite/Metal-Oxide Hybrid X-Ray Detector and Array with Decoupled Electronic and Ionic Transport Pathways.

Small Methods 2022 Jun 26:e2200500. Epub 2022 Jun 26.

State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310007, China.

Lead halide perovskites possess heavy elements and excellent mobility-lifetime (µτ) product, becoming desirable candidates for X-ray detectors. However, current perovskite photoconduction detectors (PCDs) with vertical geometry, where electronic signals and mobile ions share the same conduction path, are facing with extremely challenging ion-migration issue. Herein, a hybrid X-ray detector device structure, in which perovskite is vertically stacked onto an indium oxide (In O ) transistor with lateral transport geometry is designed, perovskite mainly acts as X-ray sensitizer to activate In O conduction channel, the actual electrical signal is conducted and collected in the lateral metal-oxide device. With the decoupled ionic and electronic transportation, hybrid detectors are insensitive to the ionic motion of perovskite, hence demonstrating no hysteresis and almost no shifting of baseline that are often observed in PCDs, hybrid detectors also exhibit reduced dark current, improved response time, and four times higher photocurrent signals. Finally, array integration of hybrid detectors and preliminary X-ray imaging is realized. The work provides an effective device strategy in addition to the mere material alternations to attain high-performance perovskite-based X-ray detectors and arrays.
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http://dx.doi.org/10.1002/smtd.202200500DOI Listing
June 2022

Effects of Substrate Roughness on Microstructure and Fatigue Behavior of Plasma Electrolytic Oxidation-Coated Ti-6Al-4V Alloy.

Materials (Basel) 2022 Jun 15;15(12). Epub 2022 Jun 15.

Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China.

Ceramic coatings were prepared by plasma electrolytic oxidation (PEO) on four different surface roughness' of Ti-6Al-4V alloys. The effects of substrate roughness on the microstructure and fatigue behavior were investigated. Microstructural characterization was carried out by scanning electron microscopy (SEM) and a laser scanning confocal microscope. In addition, an X-ray diffractometer (XRD) and a U-X360 stress meter were used to analyze the phase composition and residual stress properties of the coatings. The microstructure of coatings revealed the growth mechanism of the coatings. The larger and deeper grooves of the substrate promoted the nucleation and growth of the PEO coating, but the defects (cracks and pores) of the oxide layer became more serious. The fatigue test indicated a significant influence of substrate roughness on the fatigue life under low cyclic stress. The fatigue damage of PEO coatings decreases as the surface roughness of substrates decreases because of the synergistic effect of the coating surface defects and coating/substrate interface roughness. Substrate roughness influences the quality and fatigue performance of the oxide layer.
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http://dx.doi.org/10.3390/ma15124256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228109PMC
June 2022

Density Functional Theory Studies on the Hydrolysis of Levoglucosenone to 5-Hydroxymethylfurfural.

J Phys Chem A 2022 Jul 22;126(26):4248-4254. Epub 2022 Jun 22.

Joint International Research Laboratory of Biomass Energy and Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China.

Selective conversion of lignocellulosic biomass-derived chemicals is of critical significance for sustainable fine and commodity chemical industries. Cellulose-derived levoglucosenone (LGO) has a promising potential for producing 5-hydroxymethylfurfural (HMF) with a substantial yield under acid conditions, but the mechanism is unidentified. Herein, we disclose the mechanism of LGO conversion to HMF in the aqueous phase without and with HSO as a catalyst by density functional theory (DFT) calculations for the first time. Results showed that LGO first forms 6,8-dioxabicyclo[3.2.1]-octane-2,4,4-triol (DH) via two sequential hydration reactions occurring at the C═C bond and then the ketone group. The use of HSO as a catalyst significantly reduced the free energy barriers of LGO and DH conversion to HMF, with a free energy barrier of 115 kJ/mol for LGO → HMF compared to that of 91 kJ/mol for DH → HMF, demonstrating that DH is easier for HMF formation.
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http://dx.doi.org/10.1021/acs.jpca.2c03169DOI Listing
July 2022

A novel fluorescent probe for real-time imaging of thionitrous acid under inflammatory and oxidative conditions.

Redox Biol 2022 08 17;54:102372. Epub 2022 Jun 17.

Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University (Army Medical University), Chongqing, 400038, China. Electronic address:

Thionitrous acid (HSNO), a crosstalk intermediate of two crucial gasotransmitters nitric oxide and hydrogen sulfide, plays a critical role in redox regulation of cellular signaling and functions. However, real-time and facile detection of HSNO with high selectivity and sensitivity remains highly challenging. Herein we report a novel fluorescent probe (SNP-1) for HSNO detection. SNP-1 has a simple molecular structure, but showing strong fluorescence, a low detection limit, a broad linear detection range (from nanomolar to micromolar concentrations), ultrasensitivity, and high selectivity for HSNO in both aqueous media and cells. Benefiting from these unique features, SNP-1 could effectively visualize changes of HSNO levels in mouse models of acute ulcerative colitis and renal ischemia/reperfusion injury. Moreover, the good correlation between colonic HSNO levels and disease activity index demonstrated that HSNO is a promising new diagnostic agent for acute ulcerative colitis. Therefore, SNP-1 can serve as a useful fluorescent probe for precision detection of HSNO in various biological systems, thereby facilitating mechanistic studies, therapeutic assessment, and high-content drug screening for corresponding diseases.
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http://dx.doi.org/10.1016/j.redox.2022.102372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214870PMC
August 2022

Associations between intake of starchy and non-starchy vegetables and risk of hepatic steatosis and fibrosis.

Hepatol Int 2022 Aug 21;16(4):846-857. Epub 2022 Jun 21.

Department of Nutrition, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.

Background: Current dietary guidelines generally treat all types of vegetables the same. However, whether specific vegetables are more beneficial or deleterious for preventing chronic liver disease (CLD) remains uncertain.

Methods: We investigated the associations between starchy and non-starchy vegetables and the odds of hepatic steatosis and fibrosis in a US nationwide cross-sectional study. Diet was assessed by the 24-h dietary recalls. Hepatic steatosis and fibrosis were defined based on vibration-controlled transient elastography (TE). Multiple logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs).

Results: Among 4170 participants with reliable TE test, 1436 were diagnosed with steatosis, 255 with advanced fibrosis. Increased intake of total starchy vegetables was associated with higher odds of steatosis (OR per 1-SD increment 1.11, 95% CI 1.01-1.24) and advanced fibrosis (OR = 1.39, 95% CI 1.15-1.69). Similar positive associations were observed for potatoes. Conversely, intakes of total non-starchy (OR = 0.82, 95% CI 0.71-0.95) and dark-green vegetables (OR = 0.89, 95% CI 0.82-0.97) were inversely associated with steatosis prevalence. Replacing 5% of energy from starchy vegetables (OR = 0.65, 95% CI 0.44-0.97) or potatoes (OR = 0.65, 95% CI 0.43-0.97) with equivalent energy from dark-green vegetables was associated with lower odds of steatosis.

Conclusions: These findings support the recommendation to limit starchy vegetable intake and increase non-starchy vegetable intake in CLD prevention, and provide evidence for the potential health benefit from dietary substitution of non-starchy vegetables for starchy vegetables.
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http://dx.doi.org/10.1007/s12072-022-10368-xDOI Listing
August 2022

Case report: Type II odontoid fracture in the ankylosing spondylitis patient: Successful treatment by anterior odontoid screw fixation.

Asian J Surg 2022 Jun 16. Epub 2022 Jun 16.

Department of Orthopaedic, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.012DOI Listing
June 2022

Total Hip Arthroplasties for Hartofilakidis Type C1 and C2 High Hip Dislocations Demonstrate Similar Survivorship and Clinical Function at Minimum 10-year Follow-up With Cementless Implants.

J Arthroplasty 2022 Jun 14. Epub 2022 Jun 14.

Department of Orthopedics, General Hospital of Chinese PLA, Beijing, China; National Clinical Research Center for Orthopedics, Sports Medicine & Rehabilitation, Beijing, China.

Background: This study aimed to determine the differences in survivorship, clinical function, and complications among patients who have Hartofilakidis Type C1 or C2 developmental dysplasia of the hips and underwent total hip arthroplasty (THA) with cementless implants.

Methods: This retrospective study identified 84 Hartofilakidis Type C hips that underwent THA between 2002 and 2011 with a minimum 10-year follow-up (mean, 13 years, range, 10 to 19 years). Survivorship, latest Harris Hip Scores and satisfaction levels, radiographic outcomes (eg, implant stability, rate and length of subtrochanteric shortening transverse osteotomy, leg-length discrepancy, cup position, and orientation), as well as complications (eg, dislocation, periprosthetic fracture, periprosthetic joint infection) were compared to analyze the differences between Hartofilakidis C1 and C2 hips.

Results: Between C1 and C2 hips, no difference existed in the 15-year cumulative Kaplan-Meier survivorship, with the endpoint defined as any reoperation (93.1 versus 90.8%), aseptic loosening combined with periprosthetic joint infection (93.1 versus 96.2%), or aseptic loosening (94.8 versus 96.2%), latest Harris Hip Score (87.1 versus 86.1%), vertical (6.1 versus 6.0 mm) and horizontal (11.5 versus 10.3 mm) distance to the anatomic center of rotation, postoperative leg length discrepancy (11.2 versus 15.5 mm), dislocation (5.2 versus 11.5%), stem aseptic loosening (6.9 versus 7.7%), periprosthetic fracture (3.4 versus 7.7%), and intraoperative femoral fracture (32.8 versus 23.1%). However, Type C2 hips demonstrated more severe preoperative leg length discrepancy (66.9 versus 42.5 mm) and required a higher percentage of subtrochanteric shortening transverse osteotomies (84.6 versus 36.2%) that were longer (33.7 versus 26.47 mm) than the Type C1 hips.

Conclusion: With cementless cups positioned near the anatomic acetabular center and cementless stems combined with subtrochanteric shortening transverse osteotomies, THAs for the Hartofilakidis Type C1 and C2 hips demonstrated similar survivorship, clinical function, and complications.
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http://dx.doi.org/10.1016/j.arth.2022.06.005DOI Listing
June 2022

Shifts of the new functional marker gene (pahE) of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial population and its relationship with PAHs biodegradation.

J Hazard Mater 2022 09 6;437:129305. Epub 2022 Jun 6.

State Key Joint Laboratory on Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Identification of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial populations and understanding their responses to PAHs are crucial for the designing of appropriate bioremediation strategies. In this study, the responses of PAHs-degrading bacterial populations to different PAHs were studied in terms of the compositions and abundance variations of their new functional marker gene (pahE) by gene-targeted metagenomic and qPCR analysis. Overall, PAHs species significantly affected the composition and abundance of pahE gene within the PAHs-degrading bacteria in each treatment and different pahE of PAHs-degrading bacteria involved in the different stages of PAHs degradation. Noted that new pahE genotypes were also discovered in all PAHs treatment groups, indicating that some potential new PAHs-degrading bacterial genera were also involved in PAHs degradation. Besides, all three PAH removal rates were significantly positively related with pahE gene abundances (R = 0.908 ~ 0.922, p < 0.01), demonstrating that pahE could be a good indicator of PAHs degradation activity or potential. This is the first study focusing on the dynamic changes of the pahE gene within PAHs-degrading bacterial community during the degradation of PAHs in mangrove sediment, providing novel insights into the use of pahE gene as the functional marker to indicate PAH degradation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129305DOI Listing
September 2022

Degradation of mirubactin to multiple siderophores with varying Fe(III) chelation properties.

Org Biomol Chem 2022 06 29;20(25):5066-5070. Epub 2022 Jun 29.

Xiangya International Academy of Translational Medicine, Central South University, Changsha, Hunan, 410013, P. R. China.

Three siderophores mirubactins B-D (4-6) were identified as the degradation products of previously isolated mirubactin (1). Their structures were revealed by HR-ESI-MS/MS, NMR analyses, and density functional calculations, among which 4 contains an unusual cyclic amidine functionality. Cyclic voltammetry showed that 5 and 6 have reduced iron complexing capacity. Mirubactin (1) and Fe(III) could also form a stable complex, which may be an ingenious approach to compete for iron acquisition by the producing organisms.
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http://dx.doi.org/10.1039/d2ob00942kDOI Listing
June 2022
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