Publications by authors named "Yong Huang"

1,787 Publications

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Circular RNA ITCH Is a Tumor Suppressor in Clear Cell Renal Cell Carcinoma Metastasis through miR-106b-5p/PDCD4 Axis.

J Immunol Res 2021 20;2021:5524344. Epub 2021 Apr 20.

Department of Urology, Huai'an Second People's Hospital and The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.

High metastasis of clear cell renal cell carcinoma (ccRCC) significantly influenced survival rate of ccRCC patients. Here, we intended to investigate the impacts of circular RNA ITCH (circ-ITCH) on the metastasis of ccRCC. The expression of circ-ITCH in ccRCC tissues and cells was evaluated utilizing qRT-PCR. Transwell assay and wound healing were applied to investigate migration and invasion of ccRCC cells. Target gene prediction and screening and luciferase reporter gene assays were utilized to assess downstream target genes of circ-ITCH. Western blot was utilized to detect metastasis-related protein expression. A xenograft tumor model was established to evaluate the role of circ-ITCH in vivo. Results showed that circ-ITCH was low expressed in ccRCC tissues and cells. Downregulation circ-ITCH promoted cell migration, but overexpressing circ-ITCH inhibited cell migration and invasion in OSRC-2 and SW839 cells. Mechanism investigations claimed that circ-ITCH exerted its metastasis-inhibitory activity via sponging miR-106b-5p and regulating the expression of PDCD4. Conclusively, circ-ITCH suppresses ccRCC metastasis by enforcing PDCD4 expression through binding miR-106b-5p. circ-ITCH may function as a novel diagnostic target to suppress ccRCC metastasis.
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http://dx.doi.org/10.1155/2021/5524344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081612PMC
April 2021

Studies on the control effect of Bacillus subtilis on wheat powdery mildew.

Pest Manag Sci 2021 May 9. Epub 2021 May 9.

College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Background: Wheat powdery mildew is a worldwide fungal disease and one of the main diseases harming wheat production. Bacillus subtilis is a vital biocontrol bacteria with broad-spectrum antimicrobial activity. In this study, we systematically studied the control effect of B. subtilis on wheat powdery mildew.

Results: The control efficiency of 4×10 CFU/ml B. subtilis on wheat leaves was 71.75% in vitro and 70.31% in a pot experiment. 4×10 CFU/ml B. subtilis significantly inhibited spore germination (spore germination rate of 22.23%) and increase appresorium deformity (appresorium deformity rate of 69.33%), which was significantly different from the results in the sterile water-treatment. Through transcriptome sequencing (RNA-seq) analysis, we found that the differentially expressed genes (DEGs) were mainly enriched in the biosynthesis and metabolism of amino acids (including phenylalanine), carbon metabolism, the pentose phosphate pathway and other pathways. In particular, the plant hormone signal pathway gene nonexpressor of pathogenesis-related genes 1 (NPR1) was significantly upregulated.

Conclusion: B. subtilis at concentrations of 4 × 10 CFU/ml had a significant control effect on wheat powdery mildew and can inhibit the germination of the conidial germ tubes and the normal development of appressorium. B. subtilis may induce disease resistance in wheat to control wheat powdery mildew, and this effect is related to the SA-dependent signal pathway.
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http://dx.doi.org/10.1002/ps.6471DOI Listing
May 2021

Synthesis and preclinical evaluation of an AlF radiofluorinated bivalent PSMA ligand.

Eur J Med Chem 2021 May 1;221:113502. Epub 2021 May 1.

Department of Nuclear Medicine, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, Guangdong Province, 510515, China. Electronic address:

Prostate-specific membrane antigen (PSMA) has become as an outstanding prostate cancer-related target for diagnostic imaging and targeted radiotherapy. Clinical studies on a few PSMA radiotracers are currently underway to determine their efficacy as imaging agents to detect prostate cancer. To improve tumor retention and tumor-to-normal tissue contrast, we herein report the synthesis and preclinical evaluation of an AlF-labeled bivalent PSMA ligand (F-Bi-PSMA). F-Bi-PSMA was successful automated preparation and in vitro evaluation showed that F-Bi-PSMA was potent binding affinity, high specificity, and rapid internalization in PSMA-expressing cells. Biodistribution studies revealed a high and specific tumor uptake of 20.5 ± 3.5 %ID/g in 22Rv1 tumor-bearing mice. Furthermore, compared to the clinically used monomeric PSMA-targeting tracers, Ga-PSMA-11 and F-PSMA-1007, F-Bi-PSMA exhibited improved pharmacokinetics and higher tumor uptake, as well as better tumor-to-normal tissue contrast, resulting in considerably high imaging quality. Our findings indicated that the bivalent PSMA radioligand, F-Bi-PSMA, was successfully synthesized and ideal imaging properties.
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http://dx.doi.org/10.1016/j.ejmech.2021.113502DOI Listing
May 2021

Distinct functions of CAR-T cells possessing a dectin-1 intracellular signaling domain.

Gene Ther 2021 May 6. Epub 2021 May 6.

State Key Laboratory of Biotherapy/Collaborative Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Chimeric antigen receptor T (CAR-T) cell therapy has demonstrated remarkable efficacies in treating hematopoietic malignancies, but not in the solid tumors. Incorporating costimulatory signaling domains, such as ICOS or 4-1BB, can positively influence CAR-T cell functions and then the immune responses. These CAR-engineered T cells have showed their enhanced persistence and effector functions with improved antitumor activities, and provided a new approach for the treatment of solid tumors. Here, we designed novel 2nd generation CARs with a costimulatory signaling molecule, dectin-1. The impacts of dectin-1 signaling domain on CAR-T cells were evaluated in vitro and in vivo. Our data show that in vitro cytokine secretions by HER2 or CD19 specific CAR-T cells increase significantly via incorporating this dectin-1 signaling domain. Additional properties of these novel CAR-T cells are affected by this costimulatory domain. Compared with a popular reference (i.e., anti-HER2 CAR-T cells with 4-1BB), in vitro T cell functions and in vivo antitumor activity of the dectin-1 engineered CAR-T cells are similar to the 4-1BB based, and both are discrete to the mock T cells. Furthermore, we found that the CAR-T cells with dectin-1 show distinct phenotype and exhaustion marker expression. These collective results suggest that the incorporation of this new signaling domain, dectin-1, into the CARs may provide the clinical potential of the CAR-T cells through this signaling domain in treating solid tumors.
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http://dx.doi.org/10.1038/s41434-021-00257-7DOI Listing
May 2021

Which Criterion for Wound Drain Removal is Better Following Posterior 1-Level or 2-Level Lumbar Fusion With Instrumentation: Time Driven or Output Driven?

Global Spine J 2021 May 4:21925682211013770. Epub 2021 May 4.

Department of Spine Surgery, ZhongDa Hospital, School of Medicine, Southeast University, Nanjing, China.

Study Design: Case-control study.

Objectives: To compare the outcomes of 2 different criteria (time driven and output driven) for wound drain removal and identify which one is better.

Methods: 743 patients who underwent posterior lumbar fusion with instrumentation involving 1 or 2 motion segments were enrolled in this study. Based on the different criteria for drain removal, the patients were divided into 2 groups. The drains were discontinued by time driven (postoperative day 2) in group I and output driven (<50 ml per day) in group II. Demographic characteristics, perioperative parameters and clinical outcomes were compared between the 2 groups.

Results: The demographic characteristics in both groups were comparable. The postoperative drain output, total blood loss, postoperative timing of ambulation, and postoperative duration of hospital stay in group I were lower than those in group II ( < 0.001). There was a higher proportion of patients requiring postoperative blood transfusion in group II, but not to a level of statistical significance ( = 0.054). There was no statistical significant difference in the incidence of surgical site infection (SSI) or symptomatic spinal epidural hematoma (SEH) between the 2 groups ( > 0.05).

Conclusions: This study reveals that there are more benefits of wound drain removal by time driven than that by output driven for patients undergoing posterior 1-level or 2-level lumbar fusion with instrumentation, including less postoperative drain output, less total blood loss, earlier postoperative timing of ambulation and less postoperative duration of hospital stay without increasing the incidence of postoperative SSI or symptomatic SEH.
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http://dx.doi.org/10.1177/21925682211013770DOI Listing
May 2021

CD11c participates in triggering acute graft-versus-host disease during bone marrow transplantation.

Immunology 2021 May 2. Epub 2021 May 2.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300020, China.

CD11c is a canonical dendritic cells (DCs) marker with poorly defined functions in the immune system. Here, we found that blocking CD11c on human peripheral blood mononuclear cell derived DCs (MoDCs) inhibited the proliferation of CD4 T cells and the differentiation into IFN-γ-producing T helper 1 (Th1) cells, which were critical in acute graft-versus-host disease (aGVHD) pathogenesis. Using allogeneic bone marrow transplantation (allo-BMT) murine models, we consistently found that CD11c-deficient recipient mice had alleviated aGVHD symptoms for the decreased IFN-γ-expressing CD4 Th1 cells and CD8 T cells. Transcriptional analysis showed that CD11c participated in several immune regulation functions including maintaining antigen presentation of APCs. CD11c-deficient bone marrow-derived DCs (BMDCs) impaired the antigen presentation function in coculture assay. Mechanistically, CD11c interacted with MHCII and Hsp90 and participated in the phosphorylation of Akt and Erk1/2 in DCs after multiple inflammatory stimulations. Therefore, CD11c played crucial roles in triggering aGVHD and might serve as a potential target for the prevention and treatment of aGVHD.
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http://dx.doi.org/10.1111/imm.13350DOI Listing
May 2021

[Expert Consensus for Thermal Ablation of Pulmonary Subsolid Nodules (2021 Edition)].

Zhongguo Fei Ai Za Zhi 2021 Apr 26. Epub 2021 Apr 26.

Department of Oncology, Tengzhou Central People's Hospital, Tengzhou 277500, China.

"The Expert Group on Tumor Ablation Therapy of Chinese Medical Doctor Association, The Tumor Ablation Committee of Chinese College of Interventionalists, The Society of Tumor Ablation Therapy of Chinese Anti-Cancer Association and The Ablation Expert Committee of the Chinese Society of Clinical Oncology" have organized multidisciplinary experts to formulate the consensus for thermal ablation of pulmonary subsolid nodules or ground-glass nodule(GGN). The expert consensus reviews current literatures and provides clinical practices for thermal ablation of GGN. The main contents include: (1) clinical evaluation of GGN, (2) procedures, indications, contraindications, outcomes evaluation and related complications of thermal ablation for GGN and (3) future development directions.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.101.14DOI Listing
April 2021

Optimal stereotactic body radiotherapy dosage for hepatocellular carcinoma: a multicenter study.

Radiat Oncol 2021 Apr 21;16(1):79. Epub 2021 Apr 21.

Department of Hepatobiliary Surgery, Guangxi Medical University Cancer Hospital, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.

Background: The optimal dose and fractionation scheme of stereotactic body radiation therapy (SBRT) for hepatocellular carcinoma (HCC) remains unclear due to different tolerated liver volumes and degrees of cirrhosis. In this study, we aimed to verify the dose-survival relationship to optimize dose selection for treatment of HCC.

Methods: This multicenter retrospective study included 602 patients with HCC, treated with SBRT between January 2011 and March 2017. The SBRT dosage was classified into high dose, moderate dose, and low dose levels: SaRT (BED ≥ 100 Gy), SbRT (EQD > 74 Gy to BED < 100 Gy), and ScRT (EQD < 74 Gy). Overall survival (OS), progression-free survival (PFS), local control (LC), and intrahepatic control (IC) were evaluated in univariable and multivariable analyses.

Results: The median tumor size was 5.6 cm (interquartile range [IQR] 1.1-21.0 cm). The median follow-up time was 50.0 months (IQR 6-100 months). High radiotherapy dose correlated with better outcomes. After classifying into the SaRT, SbRT, and ScRT groups, three notably different curves were obtained for long-term post-SBRT survival and intrahepatic control. On multivariate analysis, higher radiation dose was associated with improved OS, PFS, and intrahepatic control.

Conclusions: If tolerated by normal tissue, we recommend SaRT (BED ≥ 100 Gy) as a first-line ablative dose or SbRT (EQD ≥ 74 Gy) as a second-line radical dose. Otherwise, ScRT (EQD < 74 Gy) is recommended as palliative irradiation.
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http://dx.doi.org/10.1186/s13014-021-01778-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058965PMC
April 2021

Shensu IV prevents glomerular podocyte injury in nephrotic rats via promoting lncRNA H19/DIRAS3-mediated autophagy.

Biosci Rep 2021 May;41(5)

Department of Nephrology, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.

Shensu IV is a Chinese prescription well-known for its function in treating chronic kidney diseases. However, the potential mechanisms underlying how Shensu IV exerts its effects remain unclear. In the present study, we investigated the effects of Shensu IV on glomerular podocyte injury in nephrotic rats and puromycin-induced injury in cultured podocytes, and assessed the associated molecular mechanisms. Liquid chromatography-mass spectrometry (LC-MS) results showed that the main components of Shensu IV were l-Carnitine, P-lysoPC (LPC) 16:0, Coumaroyl tyramine, Tetramethylpyrazine, LPC 18:1, Choline, (S,S)-Butane-2,3-diol, and Scopoletin. We further found that nephrotic rats displayed pathological alterations in kidney tissues and ultrastructural changes in glomerular podocytes; however, these effects were reversed with Shensu IV treatment. Compared with the control, the numbers of autophagosomes were markedly reduced in the model group, but not in the Shensu IV treatment group. Furthermore, the expression of p62 was significantly higher in the model group than in the controls, whereas the LC3-II/I ratio was significantly lower; however, these changes were not observed when Shensu IV was administered. The protective effects of Shensu IV were further confirmed in podocytes displaying puromycin-induced injury. Compared with control group, the expression of long non-coding RNA (lncRNA) H19, mTOR, p-mTOR, and p62 was significantly increased in the puromycin group, whereas that of distinct subgroup of the RAS family member 3 (DIRAS3) was significantly decreased, as was the LC3-II/I ratio. The opposite results were obtained for both shH19- and Shensu IV-treated cells. Collectively, our data demonstrated that Shensu IV can prevent glomerular podocyte injury in nephrotic rats and puromycin-treated podocytes, likely via promoting lncRNA H19/DIRAS3-regulated autophagy.
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http://dx.doi.org/10.1042/BSR20203362DOI Listing
May 2021

Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa.

Chin J Nat Med 2021 Apr;19(4):295-304

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address:

In this study, a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa (HPTLC-QDa) method for robust authentication of Ganoderma lucidum, a popular and valuable herbal medicine, has been developed. This method is simple and practical, which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface. The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid (5 : 5 : 0.2, V/V) and all bands were transferred to QDa system directly in situ using 80% methanol with 0.1% formic acid as desorption solvent. The acquired HPTLC-QDa spectra showed that luminous yellow band b3, containing ganoderic acid B/G/H and ganodeneric acid B, the major active components of Ganoderma, could be found only in G. lucidum and G. lucidum (Antler-shaped), but not in G. sinense and G. applanatum. Moreover, bands b13 and b14 with m/z 475/477 and m/z 475/491/495, respectively, could be detected in G. lucidum (Antler-shaped), but not in G. lucidum, thus allowing simple and robust authentication of G. lucidum with confused species. This method is proved to be simple, practical and reproducible, which can be extended to analyze other herbal medicines.
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http://dx.doi.org/10.1016/S1875-5364(21)60030-4DOI Listing
April 2021

Systematic analysis of JmjC gene family and stress--response expression of KDM5 subfamily genes in .

PeerJ 2021 31;9:e11137. Epub 2021 Mar 31.

Key Laboratory of Crop Epigenetic Regulation and Development, Hunan Province, Changsha, China.

Background: Jumonji C (JmjC) proteins exert critical roles in plant development and stress response through the removal of lysine methylation from histones. which originated from spontaneous hybridization by and , is the most important oilseed crop after soybean. In JmjC proteins of species, the structure and function and its relationship with the parents and model plant remain uncharacterized. Systematic identification and analysis for JmjC family in crops can facilitate the future functional characterization and oilseed crops improvement.

Methods: Basing on the conserved JmjC domain, JmjC homologs from the three species, (AA), (CC) and were identified from the database. Some methods, such as phylogenic analysis, chromosomal mapping, HMMER searching, gene structure display and Logos analysis, were used to characterize relationships of the JmjC homologs. Synonymous and nonsynonymous nucleotide substitutions were used to infer the information of gene duplication among homologs. Then, the expression levels of subfamily genes were checked under abiotic stress by qRT-PCR.

Results: Sixty-five JmjC genes were identified from genome, 29 from and 23 from . These genes were grouped into seven clades based on the phylogenetic analysis, and their catalytic activities of demethylation were predicted. The average retention rate of genes ( gene from (93.1%) and (82.6%)) exceeded whole genome level. JmjC sequences demonstrated high conservation in domain origination, chromosomal location, intron/exon number and catalytic sites. The gene duplication events were confirmed among the homologs. Many of the subfamily genes showed higher expression under drought and NaCl treatments, but only a few genes were involved in high temperature stress.

Conclusions: This study provides the first genome-wide characterization of JmjC genes in species. The exhibits higher conservation during the formation process of allotetraploid than the average retention rates of the whole genome. Furthermore, expression profiles of many genes indicated that subfamily genes are involved in stress response to salt, drought and high temperature.
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http://dx.doi.org/10.7717/peerj.11137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019318PMC
March 2021

Stereotactic body radiotherapy versus intensity-modulated radiotherapy for hepatocellular carcinoma with portal vein tumor thrombosis.

Hepatol Int 2021 Apr 5. Epub 2021 Apr 5.

Department of Radiation Oncology, Guangxi Medical University Cancer Hospital, No.71, River Road, Nanning, 530001, Guangxi Zhuang Autonomous Region, China.

Background: It is unclear whether robotic stereotactic body radiotherapy (SBRT) is superior to intensity-modulated radiotherapy (IMRT) in advanced hepatocellular carcinoma (HCC). This study aimed to compare the long-term outcomes of SBRT with those of IMRT in HCCs with portal vein tumor thrombosis (PVTT).

Methods: We retrospectively evaluated 287 HCC patients with PVTT who underwent radiotherapy between January 2000 and January 2017. Of them, 154 and 133 patients were treated with IMRT and SBRT, respectively. Overall survival (OS), progression-free survival (PFS), intrahepatic control (IC), and local control (LC) were evaluated in univariable and propensity-score matched analyses.

Results: After matching, 102 well-paired patients were selected. There was no significant difference in the 6-, 12-, 24-, and 60-month cumulative OS (73.5, 42.9, 23.6, 7.6% vs. 72.4, 45.1, 29.8, 13.2%, p = 0.151), PFS (53.9, 29.3, 21.8, 7.5% vs. 54.5, 19.3, 12.0, 9.6%, p = 0.744), IC (61.4, 45.7, 39.0, 26.8% vs. 75.1, 45.8, 35.9, 28.7%, p = 0.144), and LC (85.2, 56.5, 52.1, 47.4% vs. 87.4, 65.2, 62.1, 62.1%, p = 0.191) between the IMRT and SBRT groups. A biologically effective dose assumed at an a/b ratio of 10 (BED) of ≥ 100 Gy was the optimal cutoff for predicting the OS, PFS, IC, and LC in the patients who received SBRT.

Conclusions: When high-precision tracking technology is available, SBRT appears to be a safe and more time-efficient treatment, achieving comparable OS, PFS, IC and LC to IMRT for local advanced HCC with PVTT. A BED ≥ 100 Gy is recommended if tolerated by normal tissue.
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http://dx.doi.org/10.1007/s12072-021-10173-yDOI Listing
April 2021

The Effects of Tai Chi Exercise Among Adults With Chronic Heart Failure: An Overview of Systematic Review and Meta-Analysis.

Front Cardiovasc Med 2021 18;8:589267. Epub 2021 Mar 18.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.

Tai chi (TC) is a popular form of exercise among adults with chronic heart failure (CHF), yet services are greatly underutilized. The aim of the current study was to identify and summarize the existing evidence and to systematically determine the clinical effectiveness of Tai Chi in the management of CHF using a systematic overview. Both English and Chinese databases were searched for systematic reviews (SRs)/meta-analyses (MAs) on TC for CHF from their inception to June 2020. The methodological quality, reporting quality, and risk of bias of SRs/MAs were assessed using Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2), the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist, and Risk of Bias in Systematic reviews (ROBIS), respectively. The evidence quality of outcome measures was assessed by the Grades of Recommendations, Assessment, Development and Evaluation (GRADE). Six SRs/MAs using a quantitative synthesis to assess various outcomes of TC in CHF were included in this overview. The methodological quality, reporting quality and risk of bias of the SRs/MAs and the evidence quality of the outcome measures are generally unsatisfactory. The limitations of the past SRs/MAs included the lack of either the protocol or registration, the list of excluded studies, and the computational details of meta-analysis were inadequately reported. The critical problems were that qualitative data synthesis relied on trials with small sample sizes and critical low quality. TC may be a promising complementary treatment for CHF. However, further rigorous and comprehensive SRs/MAs and RCTs are required to provide robust evidence for definitive conclusions.
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http://dx.doi.org/10.3389/fcvm.2021.589267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012482PMC
March 2021

Identification of an Immune-Related Risk Signature Correlates With Immunophenotype and Predicts Anti-PD-L1 Efficacy of Urothelial Cancer.

Front Cell Dev Biol 2021 18;9:646982. Epub 2021 Mar 18.

Department of Urology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Immune checkpoint inhibitor (ICI) treatment has been used to treat advanced urothelial cancer. Molecular markers might improve risk stratification and prediction of ICI benefit for urothelial cancer patients. We analyzed 406 cases of bladder urothelial cancer from The Cancer Genome Atlas (TCGA) data set and identified 161 messenger RNAs (mRNAs) as differentially expressed immunity genes (DEIGs). Using the LASSO Cox regression model, an eight-mRNA-based risk signature was built. We validated the prognostic and predictive accuracy of this immune-related risk signature in 348 metastatic urothelial cancer (mUC) samples treated with anti-PD-L1 (atezolizumab) from IMvigor210. We built an immune-related risk signature based on the eight mRNAs: ANXA1, IL22, IL9R, KLRK1, LRP1, NRG3, SEMA6D, and STAP2. The eight-mRNA-based risk signature successfully categorizes patients into high-risk and low-risk groups. Overall survival was significantly different between these groups, regardless if the initial TCGA training set, the internal TCGA testing set, all TCGA set, or the ICI treatment set. The hazard ratio (HR) of the high-risk group to the low-risk group was 3.65 ( < 0.0001), 2.56 ( < 0.0001), 3.36 ( < 0.0001), and 2.42 ( = 0.0009). The risk signature was an independent prognostic factor for prediction survival. Moreover, the risk signature was related to immunity characteristics. In different tumor mutational burden (TMB) subgroups, it successfully categorizes patients into high-risk and low-risk groups, with significant differences of clinical outcome. Our eight-mRNA-based risk signature is a stable biomarker for urothelial cancer and might be able to predict which patients benefit from ICI treatment. It might play a role in precision individualized immunotherapy.
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http://dx.doi.org/10.3389/fcell.2021.646982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012532PMC
March 2021

[Identification of Chinese medicine sea snake based on cytochrome B (Cytb)].

Zhongguo Zhong Yao Za Zhi 2021 Mar;46(5):1102-1116

Guangxi University of Chinese Medicine Nanning 530200,China.

The identification of species primordium has been one of the hot issues in the identification of traditional Chinese medicine. Sea snake is one of the most valuable Chinese medicinal materials in China. In order to understand the origin and varieties of sea snake in the market, we studied the molecular identification of 46 sea snakes by cytochrome B(Cytb). After comparison and manual correction, the sequence length was 582 bp, and the content of A+T(58.9%) was higher than that of G+C(41.1%). There exist 197 variable sites and 179 parsimony-informative sites of the sequence. There are 44 kinds of sequence alignment with consistency equal to 100%, and 2 kinds equal to 96%. A total of 408 Cytb effective sequences were downloaded from GenBank database, with a total of 68 species. Phylogenetic tree of a total of 454 sea snake sequences with the samples in this study were constructed by neighbor-joining trees and Bayesian inference method, respectively, which can identify 42 samples of medicinal materials, while 4 samples can not be identified because of their low node support. The results showed that the species of the sea snake medicine were at least from 2 genera and 5 species, namely, Aipysurus eydouxii, Hydrophis curtus, H. caerulescen, H. curtus, H. ornatus and H. spiralis. This study suggested that the original species of commercial sea snake are very complex and can provide insight into the identification of sea snakes.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201221.101DOI Listing
March 2021

Emerging landscape of circHIPK3 and its role in cancer and other diseases (Review).

Mol Med Rep 2021 Jun 31;23(6). Epub 2021 Mar 31.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China.

Circular RNAs (circRNAs) are a special class of recently re‑discovered RNAs, which are covalently closed ring RNA molecules. circRNAs have been reported to possess multiple functions and are considered crucial regulators of several processes, and are therefore gaining increasing attention. In recent years, increasing evidence has shown that circRNAs are implicated in several crucial biological processes via regulation of gene expression, and their dysregulation is also associated with the development of numerous diseases, particularly acting as oncogenic or tumor‑suppressor molecules in cancer. Furthermore, circRNAs are involved in cell proliferation, differentiation, apoptosis, invasion and metastasis. In the present review, the biogenesis and functions of circRNAs are described, with a focus on the most recent research advances and the emerging roles of circular homeodomain‑interacting protein kinase 3 (circHIPK3) in human diseases. The present review may provide novel avenues for research on the roles of circHIPK3 as a clinical diagnostic and prognostic biomarker, as well as highlighting promising therapeutic targets for certain diseases and cancer.
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http://dx.doi.org/10.3892/mmr.2021.12048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025471PMC
June 2021

A Critical Overview of Systematic Reviews of Shenfu Injection for Heart Failure.

Cardiovasc Ther 2021 6;2021:8816590. Epub 2021 Mar 6.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province 510515, China.

Objectives: Shenfu Injection (SFI) was widely used in the treatment of heart failure (HF) in China. A plethora of systematic reviews/meta-analyses (SRs/MAs) has been conducted in this research area, although with scattered results. The purpose of this overview was to conduct a comprehensive review to summarize and critically evaluate the existing evidence.

Methods: Digital databases were searched for SRs/MAs up to January 28, 2021. Two authors independently screened the reviews and assessed the methodological quality of included SRs/MAs using Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2). Quality of evidence for outcomes evaluated within the reviews was appraised with the Grading of Recommendation, Assessment, Development, and Evaluation (GRADE).

Results: Thirteen SRs/MAs met the inclusion criteria. Based on AMSTAR-2, the quality of all SRs/MAs was critically low, because all of them have more than one critical domains that were unmet. Based on GRADE, the evidence quality of 24 outcome measures was low or very low, 27 outcome measures was moderate, and none outcome measure was high. Descriptive analysis showed that SFI was an effective and safe method for HF.

Conclusions: The use of SFI for the treatment of HF may be clinically effective and safe. However, this conclusion must be interpreted cautiously due to the generally low methodological quality and low evidence quality of the included SRs/MAs. More rigorously designed SRs/MAs and RCTs with high methodological quality are necessary for further proof.
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http://dx.doi.org/10.1155/2021/8816590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960068PMC
March 2021

Functional and structural analysis of cytokine-selective IL6ST defects that cause recessive hyper-IgE syndrome.

J Allergy Clin Immunol 2021 Mar 23. Epub 2021 Mar 23.

Translational Gastroenterology Unit, University of Oxford, Oxford, United Kingdom; Department of Paediatrics, University of Oxford, Oxford, United Kingdom; Oxford National Institute for Health Research Biomedical Research Centre, Oxford, United Kingdom.

Background: Biallelic variants in IL6ST, encoding GP130, cause a recessive form of hyper-IgE syndrome (HIES) characterized by high IgE level, eosinophilia, defective acute phase response, susceptibility to bacterial infections, and skeletal abnormalities due to cytokine-selective loss of function in GP130, with defective IL-6 and IL-11 and variable oncostatin M (OSM) and IL-27 levels but sparing leukemia inhibitory factor (LIF) signaling.

Objective: Our aim was to understand the functional and structural impact of recessive HIES-associated IL6ST variants.

Methods: We investigated a patient with HIES by using exome, genome, and RNA sequencing. Functional assays assessed IL-6, IL-11, IL-27, OSM, LIF, CT-1, CLC, and CNTF signaling. Molecular dynamics simulations and structural modeling of GP130 cytokine receptor complexes were performed.

Results: We identified a patient with compound heterozygous novel missense variants in IL6ST (p.Ala517Pro and the exon-skipping null variant p.Gly484_Pro518delinsArg). The p.Ala517Pro variant resulted in a more profound IL-6- and IL-11-dominated signaling defect than did the previously identified recessive HIES IL6ST variants p.Asn404Tyr and p.Pro498Leu. Molecular dynamics simulations suggested that the p.Ala517Pro and p.Asn404Tyr variants result in increased flexibility of the extracellular membrane-proximal domains of GP130. We propose a structural model that explains the cytokine selectivity of pathogenic IL6ST variants that result in recessive HIES. The variants destabilized the conformation of the hexameric cytokine receptor complexes, whereas the trimeric LIF-GP130-LIFR complex remained stable through an additional membrane-proximal interaction. Deletion of this membrane-proximal interaction site in GP130 consequently caused additional defective LIF signaling and Stüve-Wiedemann syndrome.

Conclusion: Our data provide a structural basis to understand clinical phenotypes in patients with IL6ST variants.
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http://dx.doi.org/10.1016/j.jaci.2021.02.044DOI Listing
March 2021

The Tree Shrew as a Model for Cancer Research.

Front Oncol 2021 9;11:653236. Epub 2021 Mar 9.

National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Bio-targeting Theranostics, Guangxi Medical University, Nanning, China.

Animal disease models are necessary in medical research, and an appropriate animal model is of great importance for studies about the prevention or treatment of cancer. The most important thing in the selection of animal models is to consider the similarity between animals and humans. The tree shrew () is a squirrel-like mammal which placed in the order Scandentia. Whole-genome sequencing has revealed that tree shrews are extremely similar to primate and humans than to rodents, with many highly conserved genes, which makes the data from studies that use tree shrews as models more convincing and the research outcomes more easily translatable. In tumor research, tree shrews are often used as animal models for hepatic and mammary cancers. As research has progressed, other types of tree shrew tumor models have been developed and exhibit clinical manifestations similar to those of humans. Combining the advantages of both rodents and primates, the tree shrew is expected to be the most powerful animal model for studying tumors.
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http://dx.doi.org/10.3389/fonc.2021.653236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985444PMC
March 2021

Neoadjuvant Chemoradiotherapy Does Not Contribute to Worse Survival in Pathological Node-Negative Rectal Cancer.

Front Oncol 2021 8;11:649313. Epub 2021 Mar 8.

Department of General Surgery, Jiangdu People' s Hospital Affiliated to Yangzhou University Medical School, Yangzhou, China.

The prognostic significance of ypN0 rectal cancer with comparison to pN0 disease still remains poorly defined. This study aimed to compare the prognosis of ypN0 and pN0 rectal cancer. Eligible patients were identified from the SEER18 registries research database (the latest data up to date was on April 15, 2019). Propensity score (PS) matching was usually performed to reduce the imbalance and potential confounding that were introduced by inherent differences between the groups. The cause-specific survival (CSS) was analyzed to evaluate the prognostic prediction of ypN0 and pN0 groups using the Kaplan-Meier method with the log-rank test. Cox proportional hazard model was also used to identify independent prognostic variables. In total, 26,832 patients diagnosed with pN0 or ypN0 rectal cancer were confirmed as the final cohort, including 7,237 (27.0%) patients with radiation and 19,595 (73.0%) patients without radiation prior to surgery. The median follow-up time was up to 81 months. After adjusting for other prognostic factors, neoadjuvant radiotherapy was not an independent prognostic variable of CSS (HR = 1.100, 95%CI = 0.957-1.265, = 0.180, using pN0 group as the reference). ypN0 rectal cancer was strongly associated with worse pathological diagnoses compared with pN0 rectal cancer, contributing to worse oncologic outcomes. However, the receipt of neoadjuvant chemoradiotherapy was not an independent prognostic factor of worse prognosis in pathological node-negative patients. Our study could give guidance to the treatment of ypN0 rectal cancer.
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http://dx.doi.org/10.3389/fonc.2021.649313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982457PMC
March 2021

An enhanced strategy integrating offline superimposed two-dimensional separation with mass defect filter and diagnostic ion filter: Comprehensive characterization of steroid alkaloids in Fritillariae Pallidiflorae Bulbus as a case study.

J Chromatogr A 2021 Apr 3;1643:462029. Epub 2021 Mar 3.

Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Science, Haike Road 501, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China. Electronic address:

The inherent complexity of traditional Chinese medicines necessitates the application of multi-dimensional information to accomplish comprehensive profiling and confirmative identification of their chemical components. In this study, we display an enhanced strategy by integrating offline superimposed two-dimensional separation (S-2D-LC) with mass defect filter and diagnostic ion filter to comprehensively characterize the alkaloid composition of Fritillariae Pallidiflorae Bulbus (FPB). The superimposed HILIC × RP and UPCC × RP offline two-dimensional liquid chromatography system was constructed with superior orthogonality (R=0.004 and R=0.001) for chromatographic separation. In total, 70 fractions were collected after the first-dimensional chromatographic separation (HILIC and UPCC) and then analyzed by the second-dimensional reversed phase (RP) liquid chromatography coupled with Q-TOF/MS/MS in FAST DDA acquisition mode. A four-step interpretation strategy combining mass defect filter with diagnostic ion filter was developed to rapidly characterize alkaloids in Fritillaria species. Ultimately, a sum of 529 Fritillaria alkaloids were characterized from two botanical origins of FPB. The integrated strategy is practical to efficiently expose and comprehensively characterize more trace and isomeric components in complex herbal medicines.
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http://dx.doi.org/10.1016/j.chroma.2021.462029DOI Listing
April 2021

Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.

Exp Ther Med 2021 May 25;21(5):412. Epub 2021 Feb 25.

Department of Traditional Chinese Traumatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, P.R. China.

Vasodilator-stimulated phosphoprotein (VASP) is essential for osteoclast differentiation, and reduced VASP expression results in depressed osteoclast differentiation. Previously, we demonstrated the importance of VASP and Ras-related C3 botulinum toxin substrate 1 interactions in osteosarcoma cell migration and metastasis using Mg-63 and Saos2 cells. However, the molecular details of the functional role of VASP in cell motility and migration remain to be elucidated. The present study demonstrated that VASP affects the expression of αV-integrin, tartrate-resistant acid phosphatase (TRAP) and lamellipodia protrusion in RAW 264.7 murine macrophage cells. The RAW 264.7 mouse monocyte macrophage cell line was used as an osteoclast precursor. RAW 264.7 cells were treated with 50 ng/ml of receptor activator of nuclear factor κ-Β ligand (RANKL) in order to induce cell differentiation (osteoclastogenesis). Small interfering RNA (siRNA) was used to silence VASP, and RT-PCR and western blotting were used to determine the expression for genes and proteins, respectively. TRAP staining as a histochemical marker for osteoclast and fluorescent microscopy for lamellipodia protrusion was performed. RANKL treatment significantly increased the gene and protein expression of VASP, αV-integrin and TRAP in RAW 264.7 cells. Silencing of VASP significantly reduced the RANKL-induced expression of αV-integrin, TRAP and lamellipodia protrusion. In addition, knockdown of VASP attenuated RANKL-stimulated activation of NF-κB, c-Fos and nuclear factor of activated T cells cytoplasmic 1 transcription factors, and the phosphorylation of the p65 and IκBα. These results suggest the critical role of VASP in regulating osteoclast differentiation, which should be further explored in osteosarcoma research.
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http://dx.doi.org/10.3892/etm.2021.9856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967814PMC
May 2021

[Biological Conversion Mechanism of Sulfate Reduction Ammonium Oxidation in ANAMMOX Consortia].

Huan Jing Ke Xue 2021 Mar;42(3):1477-1487

School of Environment Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

Sulfate reduction with ammonium oxidation (SRAO) in laboratory ANAMMOX reactors was considered as an autotrophic process mediated by ANAMMOX bacteria (AnAOB), in which ammonium, as an electron donor, was oxidized by the electron acceptor sulfate. This process was developed based on the transformations of nitrogenous and sulfurous compounds observed in natural environments. Reported results vary widely for conversion mole ratios (ammonium/sulfate) as do intermediate and final products of the sulfate reduction. Thus, hypotheses surrounding biological conversion pathways of ammonium and sulfate in ANAMMOX consortia are implausible. In this study, continuous reactor experiments and batch tests were conducted under micro-aerobic (-100 mV < ORP < 0 mV, 0.5 mg·L < DO < 1 mg·L), anoxic (-300 mV < ORP < -100 mV, 0.2 mg·L < DO < 0.5 mg·L) and anaerobic (ORP < -300 mV, DO < 0.2 mg·L) conditions with different inoculated sludge (ANAMMOX sludge and mixed sludge) to verify the SRAO phenomena and identify possible pathways of substrate conversion. The key findings were that SRAO occurred only where SRB existed under anoxic condition, and was absent under anaerobic conditions with ANAMMOX consortia. The analysis of the microbial community and functional gene expression showed that ammonium oxidation by AAOB coupled with sequential ANAMMOX is possibly responsible for the loss of ammonium under anoxic condition. Organic substances released through microbial decay contributed to heterotrophic sulfate conversion by SRB. AnAOB do not possess the ability to oxidize ammonium with sulfate as the electron acceptor. SRAO could, in fact, involve a combination of aerobic ammonium oxidation, ANAMMOX, and heterotrophic sulfate reduction processes, which are mediated via AOB, AnAOB, and SRB.
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http://dx.doi.org/10.13227/j.hjkx.202008105DOI Listing
March 2021

[Adsorption Behavior of Phosphate by CaO Remolded Sediment].

Huan Jing Ke Xue 2021 Mar;42(3):1424-1432

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

This study simulated the state of CaO loss after in situ coverage and examined the bottom 2 cm of sediment after restoration. Observations and elemental analysis of the sediment using scanning electron microscopy (SEM) and X-ray energy spectrometry (EDX) were also performed. The CaO remodeling notably changed the structure of the sediment; most of the organic matter and iron-manganese oxide attached to the sediment surface was removed, the porosity of the sediment particles increased, and the Ca content was also increased. CaO remodeling stabilized the endogenous phosphorus in the sediment; total phosphorous (TP) was reduced by approximately 20% and potential active phosphorus content was reduced by approximately 30%. Furthermore, the contents of Ca-P and Res-P were significantly increased. The amount of phosphorus released from the remodeled sediment under anaerobic conditions was significantly lower than the original sediment, indicating that the CaO remodeling greatly reduced the risk of endogenous phosphorus release. The Langmuir model was more suitable than the Freundlich and Dubinin-Radushkevich models for describing the isothermal adsorption behavior of the CaO remodeling, which significantly improved the adsorption capacity of the sediment with respect to phosphate from 1.44 mg·g to 20.91 mg·g. The mechanism of adsorption was switched from chemical adsorption to physicochemical adsorption. In addition, the adsorption kinetics of the CaO remodeled sediment with respect to phosphate could be best described using the quasi-second-order kinetic model.
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http://dx.doi.org/10.13227/j.hjkx.202007221DOI Listing
March 2021

[Effect of Oxidation Strengthening on In-situ Phosphorus Immobilization of Calcium Hydroxide].

Huan Jing Ke Xue 2021 Feb;42(2):723-731

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

In this research, calcium hydroxide[Ca(OH)] and hydrogen peroxide (HO) were injected into the bottom mud in the form of plum blossom scatterers to investigate the effect on the control of endogenous phosphorus. The results showed that Ca(OH) used singly effectively immobilized in the order of 90% of endogenous phosphorus approximately 20 mm below the sediment-water interface (SWI); however, at the same time, the anaerobic environment was enhanced, resulting in the transformation of stable phosphorus to easily released phosphorus and the accumulation of potential active phosphorus. Nevertheless, the addition of HO greatly reduced the amount of potential active phosphorus in deep sediments after adding Ca(OH). The vertical diffusion depth of Ca(OH) in the sediments was significantly increased, having an influence across the depth range of 0-40mm below the SWI; the improvement at depths greater than 40 mm was not notable, which was mainly attributed to an 18-fold increase of redox potential due to the addition of the oxidant. The change of phosphorus forms in the sediment also demonstrated the excellent immobilization effect of the oxidant on phosphorus. In the 0-20 mm layer, the content of readily released phosphorus decreased significantly, while compared with a control test, Ca-P increased by approximately 10%. However, at greater depths, the amount of easily released phosphorus decreased and the rate of Ca-P increase gradually slowed.
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http://dx.doi.org/10.13227/j.hjkx.202007114DOI Listing
February 2021

A tumor microenvironment-induced absorption red-shifted polymer nanoparticle for simultaneously activated photoacoustic imaging and photothermal therapy.

Sci Adv 2021 Mar 19;7(12). Epub 2021 Mar 19.

State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.

Tumor microenvironment-responsive therapy has enormous application potential in the diagnosis and treatment of cancer. The glutathione (GSH) level has been shown to be significantly increased in tumor tissues. Thus, GSH can be used as an effective endogenous molecule for diagnosis and tumor microenvironment-activated therapy. In this study, we prepared a tumor microenvironment-induced, absorption spectrum red-shifted, iron-copper co-doped polyaniline nanoparticle (Fe-Cu@PANI). The Cu(II) in this nanoparticle can undergo a redox reaction with GSH in tumors. The redox reaction induces a red shift in the absorption spectrum of the Fe-Cu@PANI nanoparticles from the visible to the near-infrared region accompanying with the etching of this nanoparticle, which simultaneously activates tumor photoacoustic imaging and photothermal therapy, thereby improving the accuracy of in vivo tumor imaging and the efficiency of photothermal therapy. The nanoparticle prepared in this study has broad application prospects in the diagnosis and treatment of cancer.
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http://dx.doi.org/10.1126/sciadv.abe3588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978424PMC
March 2021

Semisynthesis and Biological Evaluation of Platencin Thioether Derivatives: Dual FabF and FabH Inhibitors against MRSA.

ACS Med Chem Lett 2021 Mar 15;12(3):433-442. Epub 2021 Feb 15.

Xiangya International Academy of Translational Medicine at Central South University, Changsha, Hunan 410013, China.

The discovery and clinical use of multitarget monotherapeutic antibiotics is regarded as a promising approach to reduce the development of antibiotic resistance. Platencin (PTN), a potent natural antibiotic initially isolated from a soil actinomycete, targets both FabH and FabF, the initiation and elongation condensing enzymes for bacterial fatty acid biosynthesis. However, its further clinical development has been hampered by poor pharmacokinetics. Herein we report the semisynthesis and biological evaluation of platencin derivatives - with potent antibacterial activity against methicillin-resistant in vitro. Some of these PTN analogues showed similar yet distinct interactions with FabH and FabF, as shown by molecular docking, differential scanning fluorometry, and isothermal titration calorimetry. Compounds , , , and were further evaluated in a mouse peritonitis model, among which showed in vivo antibacterial activity comparable to that of PTN. Our results suggest that semisynthetic modification of PTN is a rapid route to obtain active PTN derivatives that might be further developed as promising antibiotics against drug-resistant major pathogens.
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http://dx.doi.org/10.1021/acsmedchemlett.0c00653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957939PMC
March 2021

Minimum 7-year Follow-up of A Porous Coated Trabecular Titanium Cup Manufactured with Electron Beam Melting Technique in Primary Total Hip Arthroplasty.

Orthop Surg 2021 Mar 16. Epub 2021 Mar 16.

Department of Orthopaedic Surgery, Peking University People's Hospital, Beijing, China.

Objectives: To investigate the cup survivorship, patient satisfaction level, clinical function, and radiographic outcomes of patients who underwent total hip arthroplasty (THA) using electron beam melting (EBM)-produced porous coated titanium cups at mid-term follow up.

Methods: A total of 32 patients (32 hips) from five hospitals in China who underwent primary THA using EBM-produced trabecular titanium cups between May and December 2012 were retrospectively reviewed. The inclusion criteria were: (i) patients who underwent THA with the use of EBM-produced cups with possible 7-year follow up; and (ii) patients with follow-up information, including the cup survivorship, patient satisfaction level, and clinical outcomes such as Harris hip score. The exclusion criteria were: (i) patients with neuropathic diseases; and (ii) patients who underwent THA due to neoplastic disease. Five (15.6%) patients were lost to follow up before the 7-year follow-up and, thus, were excluded; none of these patients died due to disease associated with the THA or had undergone removal of their cups as of our last evaluation. The mean age and body mass index of the patients were 59.37 (range: 38.00-69.00) years and 24.51 (range: 16.50-34.10) kg/m , respectively. Thirteen (48.1%) of the patients were female.

Results: The average duration of follow-up was 93.48 (range: 89.00-99.00) months. The median Harris hip score improved from 42.00 (interquartile range: 37.00-49.00) to 97.00 (interquartile range: 92.00-97.00) at the latest follow up (P < 0.001). A total of 18 (66.7%) patients rated their satisfaction level as very satisfied, 6 (22.2%) as satisfied, 2 (7.4%) as neutral and 1 (3.7%) as dissatisfied. No intraoperative or postoperative complications were identified. At the latest follow up, all cups were considered to have achieved osteointegration fixation, with three or more of the five signs evident in the most recent X-ray. However, three cups revealed radiolucent lines with a width of less than 1 mm. The median vertical and horizontal distances between the latest postoperative center of rotation relative to the anatomic center of rotation were 2.50 (interquartile range: -3.10, 6.94) mm superiorly and 3.26 (interquartile range: -8.12, 2.38) mm medially, respectively, at the most recent postoperative follow up. Kaplan-Meier survivorship analysis of cups, with the endpoint defined as postoperative radiolucent lines of less than 1 mm in width in at least two zones, reveals that the 8.25-year survival was 96.3% (95% confidence interval: 76.49%-99.47%).

Conclusion: The mid-term follow-up of patients who underwent primary THA using EBM-produced porous coated titanium cups demonstrated favorable patient satisfaction, good clinical function, excellent survivorship, and adequate biological fixation.
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http://dx.doi.org/10.1111/os.12846DOI Listing
March 2021

Percutaneous injection of platelet-rich plasma to treat atrophic nonunion after internal fixation of ulnar fracture: a case report.

Nagoya J Med Sci 2021 Feb;83(1):201-208

The First Clinical College, Zhejiang Chinese Medical University, Hangzhou, China.

Non-union is a serious postoperative complication of fracture. Early detection and intervention can avoid revision surgery. Platelet-rich plasma releases many active tissue factors and has potential to promote fracture healing. Percutaneous injection of platelet-rich plasma at the fracture site may avoid surgical treatment when non-union occurs. We present a case of atrophic non-union of an ulna fracture treated conservatively with percutaneous injection of platelet-rich plasma.
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http://dx.doi.org/10.18999/nagjms.83.1.201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938104PMC
February 2021