Publications by authors named "Yong Gang Wang"

127 Publications

Predictive Value of Blood Ammonia in the Prognosis of Acute Liver Failure Evaluated by Receiver Operating Characteristic Curves.

Turk J Gastroenterol 2021 02;32(2):164-168

Critical Care Center, Fifth Medical Center of Chinese PLA Hospital, Beijing, China.

Background: To investigate the predictive value of blood ammonia (BLA) quantification in the prognosis of acute liver failure (ALF).

Methods: Seventy-one patients with ALF were enrolled and BLA concentration was measured in all patients. After following up for 28 days, patients were divided into two groups: the surviving group (n = 21) and the deceased group (n = 50). An independent-samples t-test was used to compare BLA concentrations between the two groups, and receiver operating characteristic curves were used to ¬evaluate the predictive value of BLA in the prognosis of ALF. A fourfold table analysis was performed with the determined BLA cutoff value.

Results: The average concentration of BLA in the deceased group was significantly higher compared with the surviving group (144.50 µmol/L vs. 106 µmol/L, respectively; P = .035). The cutoff BLA concentration for a good ALF prognosis was 122.5 µmol/L. The area under the curve was 0.659. Both the sensitivity and specificity were >0.6. The 95% CIs for sensitivity and specificity were 0.452-0.733 and 0.477-0.878, respectively. The fourfold table analysis revealed a positive predictive value of 83.3%, a negative predictive value of 42.9%, a misdiagnosis rate of 28.6%, and an accuracy of 63.4%.

Conclusion: With a cutoff BLA concentration of 122.5 µmol/L, the prognosis of ALF could be predicted with high sensitivity and specificity, a positive predictive value, a low misdiagnosis rate, and good accuracy.
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http://dx.doi.org/10.5152/tjg.2021.19663DOI Listing
February 2021

A case of urethrovaginal fistula caused by granulomatosis with polyangiitis mimicking malignancy.

ANZ J Surg 2021 Apr 1. Epub 2021 Apr 1.

Department of Immunology, The Royal Adelaide Hospital, Adelaide, South Australia, Australia.

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http://dx.doi.org/10.1111/ans.16794DOI Listing
April 2021

Linking cortical astrocytic neogenin deficiency to the development of Moyamoya disease-like vasculopathy.

Neurobiol Dis 2021 Jul 26;154:105339. Epub 2021 Mar 26.

Department of Neurosciences, Case Western Reserve University, Cleveland, OH, USA. Electronic address:

Moyamoya-like vasculopathy, the "puff of smoke"-like small vessels in the brain, is initially identified in patients with Moyamoya disease (MMD), a rare cerebrovascular disease, and later found in patients with various types of neurological conditions, including Down syndrome, Stroke, and vascular dementia. It is thus of interest to understand how this vasculopathy is developed. Here, we provided evidence for cortical astrocytic neogenin (NEO1) deficiency to be a risk factor for its development. NEO1, a member of deleted in colorectal cancer (DCC) family netrin receptors, was reduced in brain samples of patients with MMD. Astrocytic Neo1-loss resulted in an increase of small blood vessels (BVs) selectively in the cortex. These BVs were dysfunctional, with leaky blood-brain barrier (BBB), thin arteries, and accelerated hyperplasia in veins and capillaries, resembled to the features of moyamoya-like vasculopathy. Additionally, we found that both MMD patient and Neo1 mutant mice exhibited altered gene expression in their cortex in proteins critical for not only angiogenesis [e.g., an increase in vascular endothelial growth factor (VEGFa)], but also axon guidance (e.g., netrin family proteins) and inflammation. In aggregates, these results suggest a critical role of astrocytic NEO1-loss in the development of Moyamoya-like vasculopathy, providing a mouse model for investigating mechanisms of Moyamoya-like vasculopathy.
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http://dx.doi.org/10.1016/j.nbd.2021.105339DOI Listing
July 2021

Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing.

Arch Microbiol 2021 Jul 14;203(5):2699-2709. Epub 2021 Mar 14.

Agricultural Technology Extension Center of Kang County, Longnan, 746500, Kang County, China.

In this study, a higher metal ions-resistant bacterium, Stenotrophomonas rhizophila JC1 was isolated from contaminated soil in Jinchang city, Gansu Province, China. The Pb (120 mg/L) and Cu (80 mg/L) removal rate of the strain reached at 76.9% and 83.4%, respectively. The genome comprises 4268161 bp in a circular chromosome with 67.52% G + C content and encodes 3719 proteins. The genome function analysis showed czc operon, mer operon, cop operon, arsenic detoxification system in strain JC1 were contributed to the removal of heavy metals. Three efflux systems (i.e., RND, CDF, and P-ATPase) on strain JC1 genome could trigger the removal of divalent cations from cells. cAMP pathway and ABC transporter pathway might be involved in the transport and metabolism of heavy metals. The homology analysis exhibited multi-gene families such as ABC transporters, heavy metal-associated domain, copper resistance protein, carbohydrate-binding domain were distributed across 410 orthologous groups. In addition, heavy metal-responsive transcription regulator, thioredoxin, heavy metal transport/detoxification protein, divalent-cation resistance protein CutA, arsenate reductase also played important roles in the heavy metals adsorption and detoxification process. The complete genome data provides insight into the exploration of the interaction mechanism between microorganisms and heavy metals.
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http://dx.doi.org/10.1007/s00203-021-02271-0DOI Listing
July 2021

Prevention of Na Corrosion and Dendrite Growth for Long-Life Flexible Na-Air Batteries.

ACS Cent Sci 2021 Feb 18;7(2):335-344. Epub 2021 Jan 18.

Tianjin Key Laboratory of Advanced Functional Porous Materials, Institute for New Energy Materials and Low-Carbon Technologies, School of Materials Science and Engineering, Tianjin University of Technology, Binshui Xi dao 391, Xiqing District, 300384 Tianjin, China.

Rechargeable Na-air batteries (NABs) based on abundant Na resources are generating great interest due to their high energy density and low cost. However, Na anode corrosion in ambient air and the growth of abnormal dendrites lead to insufficient cycle performance and safety hazards. Effectively protecting the Na anode from corrosion and inducing the uniform Na plating and stripping are therefore of vital importance for practical application. We herein report a NAB with in situ formed gel electrolyte and Na anode with trace residual Li. The gel electrolyte is obtained within cells through cross-linking Li ethylenediamine at the anode surface with tetraethylene glycol dimethyl ether (G4) from the liquid electrolyte. The gel can effectively prevent HO and O crossover, thus delaying Na anode corrosion and electrolyte decomposition. Na dendrite growth was suppressed by the electrostatic shield effect of Li from the modified Li layer. Benefiting from these improvements, the NAB achieves a robust cycle performance over 2000 h in opened ambient air, which is superior to previous results. Gelation of the electrolyte prevents liquid leakage during battery bending, facilitating greater cell flexibility, which could lead to the development of NABs suitable for wearable electronic devices in ambient air.
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http://dx.doi.org/10.1021/acscentsci.0c01560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908042PMC
February 2021

Intracranial chordoid glioma: A clinical, radiological and pathological study of 14 cases.

J Clin Neurosci 2020 Oct 18;80:267-273. Epub 2020 Sep 18.

Department of Neurosurgery, China National Clinical Research Center for Neurological Diseases (NCRC-ND), Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing Key Laboratory of Brian Tumor, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, PR China. Electronic address:

Background: Chordoid gliomas (CGs) are rare neuroepithelial tumors, which commonly arise from the anterior part of the third ventricle. Most studies on CGs included only one or two cases. To better understand the disease, we report 14 patients with pathologically confirmed CGs.

Method: The clinical characteristics, including radiological and histological examination, operative records, and prognoses were analyzed and reviewed.

Result: The case series included six male and eight female patients with an average age of 44.4 years. The most common preoperative symptom was headache (64.3%) and visual deterioration (57.1%). Radiological results showed that the third ventricle (12/14) was the most common site of the brain involved, and the lesions presented with solid (n = 9, 64.3%) or cystic-solid (n = 5, 35.7%) appearance. All patients were misdiagnosed as non-CG tumors. The operation approach was mainly determined by tumor location, thus trans-callosal approach (9/14) and trans-laminar terminalis approach were commonly used. Gross total resection (GTR) was achieved in all cases and none of them received any adjuvant therapy postoperatively. The most frequent postoperative complications were diabetes insipidus, electrolyte disturbance, hypopituitarism, cognitive dysfunction, and obstructive hydrocephalus. During an average follow-up period of 40.1 months, 2 cases (14.3%) were died of refractory hypopituitarism and pulmonary embolism, respectively. The preoperative symptoms and postoperative complications were all significantly improved in other 12 patients, and MRI showed no tumor recurrence.

Conclusion: According to our experience, we recommend GTR as the primary goal, which is associated with improved rates of tumor control and without increasing rates of postoperative complications.
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http://dx.doi.org/10.1016/j.jocn.2020.09.019DOI Listing
October 2020

Progress in clinical trials of cell transplantation for the treatment of spinal cord injury: how many questions remain unanswered?

Neural Regen Res 2021 Mar;16(3):405-413

Key Laboratory of Bone and Joint Diseases Research of Gansu Province, Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou, Gansu Province, China.

Spinal cord injury can lead to severe motor, sensory and autonomic nervous dysfunctions. However, there is currently no effective treatment for spinal cord injury. Neural stem cells and progenitor cells, bone marrow mesenchymal stem cells, olfactory ensheathing cells, umbilical cord blood stem cells, adipose stem cells, hematopoietic stem cells, oligodendrocyte precursor cells, macrophages and Schwann cells have been studied as potential treatments for spinal cord injury. These treatments were mainly performed in animals. However, subtle changes in sensory function, nerve root movement and pain cannot be fully investigated with animal studies. Although these cell types have shown excellent safety and effectiveness in various animal models, sufficient evidence of efficacy for clinical translation is still lacking. Cell transplantation should be combined with tissue engineering scaffolds, local drug delivery systems, postoperative adjuvant therapy and physical rehabilitation training as part of a comprehensive treatment plan to provide the possibility for patients with SCI to return to normal life. This review summarizes and analyzes the clinical trials of cell transplantation therapy in spinal cord injury, with the aim of providing a rational foundation for the development of clinical treatments for spinal cord injury.
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http://dx.doi.org/10.4103/1673-5374.293130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996007PMC
March 2021

Protective effects of Naoxintong capsule alone and in combination with ticagrelor and atorvastatin in rats with Qi deficiency and blood stasis syndrome.

Pharm Biol 2020 Dec;58(1):1006-1022

Guangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-market Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P. R. China.

Context: Naoxintong Capsule (NXT), a Chinese medicine, has been widely used for the treatment of coronary heart disease (CHD) in clinics.

Objective: This study evaluated the cardioprotective effects of NXT alone and in combination with ticagrelor (TIC) and atorvastatin (ATO).

Materials And Methods: Qi deficiency and blood stasis rats were established by 8 weeks high fat diet feeding and 16 days exhaustive swimming and randomly divided into seven groups, that is, NXT (250, 500 and 1000 mg/kg/d), TIC (20 mg/kg/d), ATO (8 mg/kg/d), NXT (500 mg/kg/d)+TIC (20 mg/kg/d) and NXT (500 mg/kg/d)+ATO (8 mg/kg/d) group, with oral administration for 12 weeks. The contents of TC, TG, LDL-C, HDL-C, IL-1β, IL-6, IL-8, TNF-α, AST, ALT, SOD, MDA, CK-MB, LDH, TXA2, PGI2, IgA, IgG, IgM and C3 in serum were measured.

Results: NXT + TIC group was significantly superior to the TIC group in decreasing the levels of TC (4.34 vs. 5.54), TG (3.37 vs. 4.66), LDL-C (1.21 vs. 1.35), LDH (4919.71vs. 5367.19) and elevating SOD level (248.54 vs. 192.04). NXT + ATO group was significantly superior to the ATO group in decreasing the levels of AST (195.931 vs. 241.63), ALT (71.26 vs. 83.16), LDH (4690.05 vs. 5285.82), TXA2 (133.73 vs. 158.67), IgG (8.08 vs. 9.80), C3 (2.03 vs. 2.35) and elevating the levels of HDL-C (1.19 vs. 0.91), SOD (241.91vs. 209.49).

Conclusions: The present findings demonstrate that the combined use of NXT with TIC and ATO had better integrated regulating effects than TIC and ATO, respectively. The mechanism of action requires further research.
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http://dx.doi.org/10.1080/13880209.2020.1821066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534269PMC
December 2020

Astrocytic neogenin/netrin-1 pathway promotes blood vessel homeostasis and function in mouse cortex.

J Clin Invest 2020 12;130(12):6490-6509

Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.

Astrocytes have multiple functions in the brain, including affecting blood vessel (BV) homeostasis and function. However, the underlying mechanisms remain elusive. Here, we provide evidence that astrocytic neogenin (NEO1), a member of deleted in colorectal cancer (DCC) family netrin receptors, is involved in blood vessel homeostasis and function. Mice with Neo1 depletion in astrocytes exhibited clustered astrocyte distribution and increased BVs in their cortices. These BVs were leaky, with reduced blood flow, disrupted vascular basement membranes (vBMs), decreased pericytes, impaired endothelial cell (EC) barrier, and elevated tip EC proliferation. Increased proliferation was also detected in cultured ECs exposed to the conditioned medium (CM) of NEO1-depleted astrocytes. Further screening for angiogenetic factors in the CM identified netrin-1 (NTN1), whose expression was decreased in NEO1-depleted cortical astrocytes. Adding NTN1 into the CM of NEO1-depleted astrocytes attenuated EC proliferation. Expressing NTN1 in NEO1 mutant cortical astrocytes ameliorated phenotypes in blood-brain barrier (BBB), EC, and astrocyte distribution. NTN1 depletion in astrocytes resulted in BV/BBB deficits in the cortex similar to those in Neo1 mutant mice. In aggregate, these results uncovered an unrecognized pathway, astrocytic NEO1 to NTN1, not only regulating astrocyte distribution, but also promoting cortical BV homeostasis and function.
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http://dx.doi.org/10.1172/JCI132372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685758PMC
December 2020

Meta-analysis of the success rate of heartbeat recovery in patients with prehospital cardiac arrest in the past 40 years in China.

Mil Med Res 2020 07 7;7(1):34. Epub 2020 Jul 7.

Beijing Chaoyang District Jiangtai Community Health Service Center, Beijing, 100016, China.

Background: Systematic evaluation of the successful heartbeat recovery rate (HRR) in patients during the platinum ten minutes after cardiac arrest.

Methods: The databases of CNKI (January 1979-March 2019), Chongqing VIP (January 1989-March 2019), Wanfang (January 1990-March 2019) and Web of Science (1900-May 2020) were searched. To collect the clinical data of patients with cardiac arrest before hospitalization and analyze the cardiopulmonary resuscitation (CPR) at different times. Literature selection and data extraction were carried out by two researchers independently, and the meta package of R software (version 3. 61) was used for analysis.

Results: A total of 116 papers met the inclusion criteria, including 37,181 patients. Of these patients, 3367 had their heartbeats successfully restored. The results showed a high degree of heterogeneity (χ = 6999.21, P < 0.01, I = 97.6%). The meta-analysis was conducted using a random-effects model. The combined effect size was 0.199 (0.157-0.250). (1) According to the five CPR groups (International Cardiopulmonary Resuscitation Guide 2000, 2005, 2010, 2015 and other versions), the HRR of other versions [0.264 (0.176-0.375)] was higher than the International Cardiopulmonary Resuscitation 2005 edition [0.121 (0.092-0.158)]. (2) The rescue time was divided into the 0 to ≤5 min group, the 5 to ≤10 min group, the 10 to ≤15 min group, and the > 15 min group. The HRR were 0.417 (0.341-0.496), 0.143 (0.104-0.193), 0.049 (0.034-0.069), and 0.022 (0.009-0.051), respectively. The HRR was higher in the 0 to ≤5 min group than in the 5 to ≤10 min group, the 10 to ≤15 min group and the > 15 min group. There was no difference between the 10 to ≤15 min group and the > 15 min group. (3) When the groups were stratified with the cutoff of 10 min, the ≤10 min group HRR [0.250 (0.202-0.306)] was higher than the > 10 min group rate [0.041 (0.029-0.057)]. (4) The HRR of the telephone guidance group was [0.273 (0.227-0.325)] lower than that of the 0 to ≤5 min group [0.429 (0.347-0.516)] but higher than that of the 5 to ≤10 min group, the 10 to ≤15 min group, and the > 15 min group. (5) The HRR of the witness group [0.325 (0.216-0.458)] was not different from that of the 0 to ≤5 min group, but it was higher than those of the 5 to ≤10 min group, the 10 to ≤15 min group and the > 15 min group. (6) There was no significant difference HRR between the witnessed group, the telephone guidance group and the ≤10 min group.

Conclusions: (1) The HRR is time-sensitive, and early rescue can improve it. (2) CPR performed within the platinum ten minutes must be executed by the public, and other forces are auxiliary. (3) The concept of peri-cardiac arrest period (PCAP) should be established and improved to guide CPR.
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http://dx.doi.org/10.1186/s40779-020-00263-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339510PMC
July 2020

[Soil fertility characteristics and their influencing factors in tea plantations of Jiangxi Pro-vince, China].

Ying Yong Sheng Tai Xue Bao 2020 Apr;31(4):1163-1174

Wuyuan Bureau of Tea, Wuyuan 333200, Jiangxi, China.

To investigate soil fertility status and characteristics of typical tea plantations, we selec-ted 372 typical tea plantations of 21 areas across Jiangxi Province and analyzed the soil nutrient, spatial data, and their correlations with topography, soil type, elevation and plantation age. The results showed that soil pH, organic matter, alkaline nitrogen, available phosphorus, available potassium, total nitrogen, total phosphorus and total potassium of tea plantation in Jiangxi reached 53.9%, 60.1%, 56.1%, 22.9%, 38.5%, 43.7%, 11.1% and 95.5% of indices of high fertility, high efficiency and high yield tea plantation, respectively, with the available phosphorus showing a strong variation. Soil available copper, zinc, iron, manganese and boron reached 76.3%, 74.2%, 96.8%, 73.1% and 0.0% of the first-class standards for soil trace elements, respectively. Tea plantations with highest soil fertility located in central Jiangxi, followed by northeastern and northwestern Jiangxi, and lowest in southern Jiangxi. Soil pH was significantly positively correlated with organic matter, alkaline nitrogen, available phosphorus, available potassium, total nitrogen and total phosphorus but not for total potassium. For different topography, soil fertility was highest in the flat land, followed by the high mountains, and lowest in the mountains and hills. Across different soil types, soil fertility was higher in paddy soil, sandy soil and mountain yellow brown soil, followed by yellow soil, red-yellow soil and purple soil, and lowest in red soil. Soil pH, organic matter and total potassium increased while available phosphorus decreased with altitude. The organic matter, alkaline nitrogen, available phosphorus, total nitrogen and total phosphorus increased, but soil pH decreased with time. In summary, soil fertility of tea plantations in Jiangxi Province was generally good, with high organic matter, total potassium, available copper, zinc, iron and manganese. However, soil was acidic, available phosphorus and total phosphorus content was low, available boron was seriously limited. We suggest increase soil pH and potassium supply in central Jiangxi, increase potassium and nitrogen fertilizer supply in northeastern Jiangxi, increase organic matter and phosphorus fertilizer supply in northwestern Jiangxi, and increase nitrogen, phosphorus and potassium supply combined with organic fertilizers in southern Jiangxi. High mountain tea plantations should enhance available phosphorus and potassium supply. Mountain tea plantations should enhance nitrogen and phosphate supply. Tea plantations with red and yellow soil should increase pH and total potassium supply. Tea plantations with red soil should apply nitrogen, phosphorus and potassium fertilizers combined with organic fertilizers. Tea plantations with yellow soil and mountain yellow brown soil required additional phosphorus supply, and tea plantations with purple soil should increase soil organic matter supply. Tea plantations need to increase dolomite powder, physiological alkaline fertilizers and organic fertilizers to prevent soil acidification.
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http://dx.doi.org/10.13287/j.1001-9332.202004.022DOI Listing
April 2020

A rapid response team is associated with reduced overall hospital mortality in a Chinese tertiary hospital: a 9-year cohort study.

Ann Transl Med 2020 Mar;8(6):317

Department of Critical Care Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.

Background: Although the evidence for its effectiveness remains uncertainty, rapid response systems are implemented across many hospitals across the world. Increasingly, hospitals in China have recently started to adopt a medical emergency or rapid response team (RRT). Hence, we aimed to determine whether the implementation of an RRT in Chinese hospitals also improved outcomes.

Methods: Our hospital is a Joint Commission International (JCI) accredited, tertiary teaching hospital with 1,200 beds. We conducted a retrospective cohort study comparing 60 months after the implementation of the RRT (January 1, 2013, to December 31, 2017) and 36 months before implementation (January 1, 2009, to December 31, 2011). The outcomes included the overall hospital mortality and incidence of codes.

Results: We analyzed 144,673 non-obstetric hospital admissions and 1,269,621 patient days in the control period and 348,687 non-obstetric hospital admissions and 2,361,913 patient days after the RRT implementation. The RRT was activated 834 times (2.39 calls per 1,000 patients and 0.35 call per 1,000 patient-days). There was no difference in the code rate (0.23 0.17 per 1,000 patient days, P=0.379) between the two periods. Although the hospital mortality had remained stable around 3.0 per 1,000 patients from 2009 to 2011, there was a significant 40% decrease of overall hospital mortality from 2.95 to 1.77 per 1,000 non-obstetric patients after the implementation of RRT (P=0.001), and the annual mortality showed a consistent decrease (P=0.037 for the trend). Moreover, the increase of RRT activations was significantly correlated with the decrease of hospital mortality (P=0.025).

Conclusions: RRT implementation was associated with reduced overall hospital mortality in a Chinese tertiary hospital.
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http://dx.doi.org/10.21037/atm.2020.02.147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186685PMC
March 2020

Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex.

J Neuroinflammation 2019 Nov 26;16(1):235. Epub 2019 Nov 26.

Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.

Background: Vacuolar sorting protein 35 (VPS35), a critical component of retromer, is essential for selective endosome-to-Golgi retrieval of membrane proteins. It is highly expressed in microglial cells, in addition to neurons. We have previously demonstrated microglial VPS35's functions in preventing hippocampal, but not cortical, microglial activation, and in promoting adult hippocampal neurogenesis. However, microglial VPS35's role in the cortex in response to ischemic stroke remains largely unclear.

Methods: We used mice with VPS35 cKO (conditional knockout) in microglial cells and examined and compared their responses to ischemic stroke with control mice. The brain damage, cell death, changes in glial cells and gene expression, and sensorimotor deficits were assessed by a combination of immunohistochemical and immunofluorescence staining, RT-PCR, Western blot, and neurological functional behavior tests.

Results: We found that microglial VPS35 loss results in an increase of anti-inflammatory microglia in mouse cortex after ischemic stroke. The ischemic stroke-induced brain injury phenotypes, including brain damage, neuronal death, and sensorimotor deficits, were all attenuated by microglial VPS35-deficiency. Further analysis of protein expression changes revealed a reduction in CX3CR1 (CX3C chemokine receptor 1) in microglial VPS35-deficient cortex after ischemic stroke, implicating CX3CR1 as a potential cargo of VPS35 in this event.

Conclusion: Together, these results reveal an unrecognized function of microglial VPS35 in enhancing ischemic brain injury-induced inflammatory microglia, but suppressing the injury-induced anti-inflammatory microglia. Consequently, microglial VPS35 cKO mice exhibit attenuation of ischemic brain injury response.
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http://dx.doi.org/10.1186/s12974-019-1633-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880612PMC
November 2019

Complete genome sequence of Acinetobacter baumanni J1, a quorum sensing-producing algicidal bacterium, isolated from Eastern Pacific Ocean.

Mar Genomics 2020 Aug 31;52:100719. Epub 2019 Oct 31.

The Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, Guangdong Province, PR China. Electronic address:

The Acinetobacter baumanni J1 isolated from surface water of the Eastern Pacific Ocean, demonstrated significant algicidal activity on the algae Alexandrium tamarense. Interestingly, this strain showed the ability to produce an acyl-homoserine lactone (AHL) quorum sensing molecule. To better understand its AHL producing mechanism and its ecological functions, the genome of A. baumanni strain J1 was completely sequenced. The genome contained a circular chromosome of 3,948,465 bp with an average GC content of 39.9 mol%. A total of 3707 protein coding genes, 41 tRNA genes and 16 rRNA genes were obtained. In silico genome annotation identified a LuxI putative gene located on contig 4. Subsequent thin-layer chromatography analysis indicated that C8-AHL could be produced by A. baumanni J1, which confirmed the authenticity of the LuxI gene. Taken together, this work describes an algicidal bacterium that is capable of producing an AHL molecule, which may represent a valuable tool for developing microbial methods to control harmful algae.
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http://dx.doi.org/10.1016/j.margen.2019.100719DOI Listing
August 2020

CNT-Decorated NaMnCo(PO)PO Microspheres as a Novel High-Voltage Cathode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2019 Aug 22;11(31):27813-27822. Epub 2019 Jul 22.

Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Institute of New Energy , Fudan University , Shanghai 200433 , China.

The Mn-based mixed polyanion is expected to be a promising cathode material for sodium-ion batteries applied to large-scale smart grid energy storage systems due to its stable three-dimensional crystal structure, low cost, and high energy density. Herein, a novel carbon nanotube (CNT)-modified mixed-polyanion material (NaMnCo(PO)PO) with a high voltage of 3.86 V is synthesized by a facile spray-drying method. The well-designed NaMnCo(PO)PO/C-CNTs microsphere has excellent electronic and ionic conductivity by virtue of the carbon nanotube conductive skeleton. The as-prepared NaMnCo(PO)PO/C-CNTs composite exhibits a reversible initial discharge capacity of 96.1 mA h g and an energy density of 371 Wh kg at 0.1 C. Furthermore, NaMnCo(PO)PO/C-CNTs and hard carbon are assembled into a full battery, which delivers an initial discharge capacity of 88.8 mA h g, a working voltage of 3.85 V, and a promising energy density of 249.9 Wh kg at 0.1 C. Therefore, the outstanding performance makes the NaMnCo(PO)PO/C-CNTs material a potential candidate for large-scale applications of sodium-ion batteries.
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http://dx.doi.org/10.1021/acsami.9b07595DOI Listing
August 2019

Predictors of survival in non-small cell lung cancer patients with pleural effusion undergoing thoracoscopy.

Thorac Cancer 2019 06 15;10(6):1412-1418. Epub 2019 May 15.

Department of Respiratory and Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

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http://dx.doi.org/10.1111/1759-7714.13086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558471PMC
June 2019

Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b.

Acta Pharmacol Sin 2019 Sep 26;40(9):1228-1236. Epub 2019 Apr 26.

Department of Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Bufalin, the major active component of the traditional Chinese medicine ChanSu obtained from the skin and parotid venom glands of toads, has long been known as an anticancer agent. Recent studies show that microRNAs (miRs) are involved in the anticancer activities of bufalin, while long non-coding RNAs (lncRNAs) are known to interact with miRNAs to regulate various biological functions. In this paper, we investigated the possible network related to the antimetastatic effect of bufalin in prostate cancer (PCa) cells. We demonstrated that bufalin (0.05-10 µM) dose-dependently suppressed the proliferation of prostate cancer DU145 and PC3 cells with IC values of 0.89 and 1.28 µM, respectively. Furthermore, bufalin treatment significantly suppressed the cell migration and invasion. To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells. Overexpression of HOTAIR counteracted the suppressing effects of bufalin on DU145 and PC3 cells. We then predicted and verified that HOTAIR upregulated FGFR1 expression by sponging miR-520b in PCa cells. In 40 patients with PCa bone metastasis, we used in situ hybridization or immunohistochemical assay to assess the HOTAIR and FGFR1 expression, which revealed that both HOTAIR and FGFR1 expression were significantly higher in bone metastasis tissues than in the primary PCa tissues. In addition, the level of serum HOTAIR was positively associated with the levels of serum bone metabolic markers (CTx, OST, B-ALP and PINP) and may serve as a reasonable biomarker for PCa bone metastasis. Taken together, this is the first study revealing that HOTAIR promotes PCa bone metastasis, and bufalin may be a promising candidate for the treatment of this disease.
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http://dx.doi.org/10.1038/s41401-019-0234-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786369PMC
September 2019

Comparison of neuroendocrine dysfunction in patients with adamantinomatous and papillary craniopharyngiomas.

Exp Ther Med 2019 Jan 12;17(1):51-56. Epub 2018 Nov 12.

Department of Endocrinology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.

Craniopharyngiomas (CPs) are rare epithelial tumors mainly located in the sellar/parasellar region. They may be classified into two major histological subtypes, which exhibit clinical and pathological differences: Adamantinomatous CP (ACP) and papillary CP (PCP). The aim of the present study was to compare the neuroendocrine dysfunction between ACP and PCP prior to and after surgical resection. According to their pathological classification, the patients were assigned to the ACP group or the PCP group. The neuroendocrine dysfunction in these two CP subtypes was evaluated using a scoring method prior to and after surgery. A total of 741 patients with CPs were included in the present study, of which 622 were ACP and 119 were PCP. The scores on the tumor mass effect, hypothalamic dysfunction and pituitary-target gland axis dysfunction, as well as the incidence of central diabetes insipidus in the PCP group were all significantly higher than those in the ACP group, regardless of whether the surgery had been performed or not (all P<0.05). After surgery, the scores on the tumor mass effect were significantly decreased in the ACP and PCP patients (all P<0.05), while the scores on hypothalamic dysfunction and pituitary-target gland axis dysfunction, as well as the incidence of central diabetes insipidus were all significantly increased in ACP and PCP patients (all P<0.05). Prior to and after surgery, the PCP variant exhibited a greater damage to the neuroendocrine function compared with the ACP variant.
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http://dx.doi.org/10.3892/etm.2018.6953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307520PMC
January 2019

Robust Negative Electrode Materials Derived from Carbon Dots and Porous Hydrogels for High-Performance Hybrid Supercapacitors.

Adv Mater 2019 Feb 9;31(5):e1806197. Epub 2018 Dec 9.

Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Laboratory of Advanced Materials, Fudan University, Shanghai, 200438, P. R. China.

Hybrid supercapacitors generally show high power and long life spans but inferior energy densities, which are mainly caused by carbon negative electrodes with low specific capacitances. To improve the energy densities, the traditional methods include optimizing pore structures and modifying pseudocapacitive groups on the carbon materials. Here, another promising way is suggested, which has no adverse effects to the carbon materials, that is, constructing electron-rich regions on the electrode surfaces for absorbing cations as much as possible. For this aim, a series of hierarchical porous carbon materials are produced by calcinating carbon dots-hydrogel composites, which have controllable surface states including electron-rich regions. The optimal sample is employed as the negative electrode to fabricate hybrid supercapacitors, which show remarkable specific energy densities (up to 62.8-90.1 Wh kg ) in different systems.
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http://dx.doi.org/10.1002/adma.201806197DOI Listing
February 2019

Black Phosphorus Stabilizing NaTiO/C Each Other with an Improved Electrochemical Property for Sodium-Ion Storage.

ACS Appl Mater Interfaces 2018 Oct 19;10(43):37163-37171. Epub 2018 Oct 19.

Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Institute of New Energy , Fudan University , Shanghai 200433 , China.

Sodium-ion batteries have increasingly been considered as an attractive alternative to lithium-ion batteries for large-scale applications. High specific capacity and suitable working potential anode materials are one of the keys to search for future developments. Here, a novel and stable sodium titanate/carbon-black phosphorus (NTO/C-BP) hybrids are first fabricated as a promising anode material for advanced sodium-ion batteries. Under the protection of argon (Ar) atmosphere, the direct high-energy mechanical milling of the BP nanoparticle and NTO/C results in the formation of NTO/C-BP hybrids. In other words, the BP nanoparticle can be interconnected with bare NTO by P-O-Ti bonds and/or form stable P-C bonds with the carbon coating layer on the surface of NTO. The NTO/C-BP hybrids are not only beneficial for enhancing specific capacity but also have a great protective effect on the exposure of BP to air by the synergistic effect between BP and NTO/C. The results show that the NTO/C-BP hybrids can deliver very high specific capacity (∼225 mA h g after 55 cycles at 20 mA g, ∼183 mA h g after 100 cycles at 100 mA g). It is expected from these scientific findings that forming stable P-C bonds and P-O-Ti bonds in this work can serve as a guidance to other Ti-based and P-based electrode materials for practical large-scale application of sodium-ion batteries.
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http://dx.doi.org/10.1021/acsami.8b14971DOI Listing
October 2018

NMDA receptors inhibit axonal outgrowth by inactivating Akt and activating GSK-3β via calcineurin in cultured immature hippocampal neurons.

Exp Cell Res 2018 10 31;371(2):389-398. Epub 2018 Aug 31.

Institute of Neuroscience and Institute of Hypoxia Medicine, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Neurons are highly polarized cells with an axon and dendritic arbors. It is still not well studied that how formation and elaboration of axon and dendrites is controlled by diffusible signaling factors such as glutamate via specific receptors. We found that N-methyl-D-aspartate (NMDA) receptors were enriched (stage 2-3) but decreased expression (stage 4-5) at tip of axon of cultured hippocampal neurons during distinct development stages. Inhibition of NMDA receptor activity by competitive antagonist DL-2-amino-5-phosphonovalerate (APV) or channel blocker MK801 promoted axonal outgrowth at the early stages, whereas inhibited dendritic development in later stages. Meanwhile, knockdown of NMDA receptors also promoted axonal outgrowth and branch in immature neurons. Furthermore, GluN2B but not GluN2A subunit inhibited axonal outgrowth in immature hippocampal neurons. Finally, we found that NMDA receptors inhibited axonal outgrowth by inactivating Akt and activating GSK-3β signaling in a calcineurin-dependent manner. Taken together, our results demonstrate that stabilization GSK-3β activation in the axon growth cone by Ca influx through NMDA receptors may be involved in regulation of axon formation in immature neurons at early stages.
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http://dx.doi.org/10.1016/j.yexcr.2018.08.033DOI Listing
October 2018

High 28-day mortality in critically ill patients with sepsis and concomitant active cancer.

J Int Med Res 2018 Dec 8;46(12):5030-5039. Epub 2018 Aug 8.

2 Department of Neurocritical Care Medicine, 2nd Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Objective: This study was performed to explore the characteristics and outcomes of patients with sepsis accompanied by active cancer who were admitted to the intensive care unit (ICU).

Methods: The baseline characteristics, infection profiles, and outcomes of patients with sepsis were retrospectively analyzed according to the presence of concomitant active cancer. The association between concomitant active cancer and 28-day mortality was explored.

Results: Of 23,956 patients with sepsis, 1574 (6.6%) had concomitant active cancer. The most common type was digestive (30.7%). The 28-day mortality ranged from 41.9% to 81.5%. Patients with active cancer had a significantly higher Simplified Acute Physiology Score II and significantly shorter length of ICU stay. Respiratory (32.9%), genitourinary (31.0%), and bloodstream (17.0%) infections were most common. Escherichia coli was the most frequent gram-negative pathogenic bacteria. The 28-day mortality rate was significantly higher in patients with than without active cancer. Concomitant active cancer was associated with increased 28-day mortality in patients with sepsis. Hematological malignancy was associated with a significantly higher risk of death than solid tumors.

Conclusions: Concomitant active cancer was associated with higher 28-day mortality in patients with sepsis requiring ICU admission. Hematological malignancy was associated with a higher risk of death than solid tumors.
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http://dx.doi.org/10.1177/0300060518789040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300972PMC
December 2018

Development and validation of a nomogram for predicting survival in patients with gastrointestinal stromal tumours.

Eur J Surg Oncol 2018 10 20;44(10):1657-1665. Epub 2018 Jul 20.

Department of Intervention and Vascular Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou Cancer Medical Center, Suzhou, Jiangsu, 215001, China. Electronic address:

Background: This study aimed to develop and validate nomograms for predicting long-term overall survival (OS) and cancer-specific survival (CSS) in gastrointestinal stromal tumours (GISTs).

Methods: Patients diagnosed with GISTs between 2004 and 2015 were selected for the study from the Surveillance, Epidemiology, and End Results (SEER) database. Patients were randomly separated into the training set and the validation set. Multivariate analysis was used on the training set to obtain independent prognostic factors to build nomograms for predicting 3- and 5-year OS and CSS. The discrimination and calibration plots were used to evaluate the predictive accuracy of the nomograms.

Results: Data for a total of 5622 patients with GISTs were collected from the SEER database. Nomograms were established based on variables that were significantly associated with OS and CSS identified by the Cox regression model. The nomograms for predicting OS and CSS displayed better discrimination power than did the SEER stage and Tumour-Node-Metastasis (TNM) staging systems (7th edition) in the training set and validation set. Calibration plots of the nomograms indicated that OS and CSS closely corresponded to actual observation.

Conclusions: The nomograms were able to more accurately predict 3- and 5-year OS and CSS of patients with GISTs than were existing models.
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http://dx.doi.org/10.1016/j.ejso.2018.07.004DOI Listing
October 2018

Long-term survival of the middle and lower thoracic esophageal cancer patients after surgical treatment through left or right thoracic approach.

J Thorac Dis 2018 May;10(5):2648-2655

Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Background: Esophageal cancer is one of the most prevalent malignancies with a high incidence and mortality in China, the main treatment for esophageal cancer at present is still surgery-based multimodality treatment, and surgery is still the most effective measure. However, the modes of surgical treatment for esophageal cancer have been diverse. The surgical approaches can be mainly divided into the left thoracic approach and right thoracic approach in China. The long-term survival of the patients treated through right approach was reported better than that through left thoracic approach, but until now no statistically significant difference was found between two approaches, especially, for those with middle and lower thoracic esophageal cancer without suspected upper mediastinal lymph node metastasis in preoperative examinations, no definite conclusion have been made on selection of the approach, therefore, this studies try to compare the long-term survival between two approaches .

Methods: The data of 402 cases with complete resection and two-field lymph node dissection from January, 2011 to December, 2011 in the Cancer Hospital, Chinese Academy of Medical Sciences was retrospectively reviewed and analyzed. Propensity score matching (PSM) analysis and life-table in SPSS 22.0 and Stata 14.0 were used to analyze the survival.

Results: Totally, 402 cases were surgically treated either via left or right thoracic approach. The overall 5-year survival rate of this series was 38%, it was 37% in 281 cases surgically treated through left approach, and 39% in 121 cases through right approach (P=0.908). The 5-year survival of 256 patients without suspected lymph node metastasis in the upper mediastinum based on the preoperative examinations surgically treated through left approach was 38% versus 43% of 88 cases through right approach (P=0.404). After PSM, the 5-year survival of 110 cases surgically treated through left approach was 32% versus 40% of another matched 110 cases through right approach (P=0.146). for the patients without suspected lymph node metastasis in the upper mediastinum based on preoperative examinations, the 5-year survival of 88 surgically treated through left approach was 33% versus 44% of another matched 88 cases through right approach (P=0.239).

Conclusions: For the middle and lower thoracic esophageal cancer patients, whether or not who has suspected lymph node metastasis in the upper mediastinum based on preoperative CT and EUS, the surgical treatment through right thoracic approach can achieve better but not significantly better overall survival than that through left thoracic approach. Further prospective randomized clinical trials are still needed to verify this disputed issue on approach selection.
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http://dx.doi.org/10.21037/jtd.2018.04.45DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006125PMC
May 2018

A nomogram to predict overall survival for biliary tract cancer.

Cancer Manag Res 2018 14;10:1535-1541. Epub 2018 Jun 14.

Department of Intervention and Vascular Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou Cancer Medical Center, Suzhou, China.

Background: The aim of the study was to develop and validate a nomogram to predict overall survival (OS) in biliary tract cancer (BTC).

Patients And Methods: Patients diagnosed with BTC between 2004 and 2014 were selected for the study from the Surveillance, Epidemiology, and End Results (SEER) database. All patients were randomly allocated to 2 sets, the training set (n = 8,869) and the validation set (n = 8,766), for the purposes of validation. The prognostic effects of each variable were examined using univariate and multivariate analyses. Cox regression models and a nomogram were developed based on significant prognostic factors. The predictive and discriminatory capacity of the nomogram was evaluated by Harrell's concordance index (C-index) and calibration plots.

Results: Data of 17,635 patients with BTC were collected from the SEER database. Age; race; tumor site; tumor grade; T, N, and M stage; marital status; and therapy were associated with survival in the multivariate models. All these factors were integrated to construct the nomogram. The nomogram for predicting OS displayed better discrimination power than the tumor-node-metastasis (TNM) stage system 6th edition in the training set and validation set. The calibration curve indicated that the nomogram was able to accurately predict 3- and 5-year OS.

Conclusion: This predictive model has the potential to provide an individualized risk estimate of survival in patients with BTC.
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http://dx.doi.org/10.2147/CMAR.S163291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005298PMC
June 2018

Hyperbaric oxygen inhibits production of CD3+ T cells in the thymus and facilitates malignant glioma cell growth.

J Int Med Res 2018 Jul 22;46(7):2780-2791. Epub 2018 May 22.

1 Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.

Objective Hyperbaric oxygen (HBO) is an emerging complementary alternative medical approach in glioma treatment. However, its mode of action is unknown, so this was investigated in the present study. Methods We constructed an intracranial glioma model of congenic C57BL/6J mice. Glioma growth under HBO stimulation was assessed by bioluminescent imaging and magnetic resonance imaging. Flow cytometry assessed direct effects of HBO on reactive oxygen species (ROS) signaling of transplanted glioma cells and organs, and quantified mature T cells and subgroups in tumors, the brain, and blood. Results HBO promoted the growth of transplanted GL261-Luc glioma in the intracranial glioma mouse model. ROS signaling of glioma cells and brain cells was significantly downregulated under HBO stimulation, but thymus ROS levels were significantly upregulated. CD3+ T cells were significantly downregulated, while both Ti/Th cells (CD3+CD4+) and Ts/Tc cells (CD3+CD8+) were inhibited in tumors of the HBO group. The percentage of regulatory T cells in Ti/Th (CD3+CD4+) cells was elevated in the tumors and thymuses of the HBO group. Conclusion HBO induced ROS signaling in the thymus, inhibited CD3+ T cell generation, and facilitated malignant glioma cell growth in vivo in the intracranial glioma mouse model.
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http://dx.doi.org/10.1177/0300060518767796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124287PMC
July 2018

Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo.

Molecules 2018 May 16;23(5). Epub 2018 May 16.

Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post-Marketed TCM, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

is traditionally used as a folk medicine to treat cholestasis and hepatitis. Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of and has been rarely studied. The aim of the present study was to evaluate the antioxidant activity and hepatoprotective potential of Q7R. In the in vitro experiments, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assays were first performed to assess the antioxidant properties of Q7R, and then a H₂O₂-induced oxidative damage cellular model was used to determine the cytoprotective and antioxidant properties of Q7R in human liver L-02 cells. In the in vivo experiment, the hepatoprotective activity of Q7R was evaluated by carbon tetrachloride (CCl₄)-induced liver damage model in mice. The results of the three in vitro assays (DPPH, ABTS and FRAP) demonstrated that Q7R significantly exhibited antioxidant activity. The cell experiment results showed that Q7R possessed cytoprotective and antioxidant effects on H₂O₂-treated L-02 cells. In the in vivo experiments, Q7R suppressed the up-regulation of serum activities of ALT, AST, LDH and triglyceride (TG) levels with dose-dependency. Q7R down-regulated the production of MDA and increased the hepatic GSH content and antioxidant enzymes CAT activities. Hepatic morphological analysis was also performed to confirm the biochemical changes. In summary, these results suggested that Q7R could be considered as a potential source of natural antioxidants, and may become a promising candidate for the treatment of liver injury in the future.
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http://dx.doi.org/10.3390/molecules23051188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100316PMC
May 2018

Chromatogram-Bioactivity Correlation-Based Discovery and Identification of Three Bioactive Compounds Affecting Endothelial Function in Ginkgo Biloba Extract.

Molecules 2018 05 3;23(5). Epub 2018 May 3.

Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed TCM, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 135 Xingangxi Road, Guangzhou 510275, China.

Discovery and identification of three bioactive compounds affecting endothelial function in Extract (GBE) based on chromatogram-bioactivity correlation analysis. Three portions were separated from GBE via D101 macroporous resin and then re-combined to prepare nine GBE samples. 21 compounds in GBE samples were identified through UFLC-DAD-Q-TOF-MS/MS. Correlation analysis between compounds differences and endothelin-1 (ET-1) in vivo in nine GBE samples was conducted. The analysis results indicated that three bioactive compounds had close relevance to ET-1: Kaempferol-3--α-l-glucoside, 3--{2--{6--[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Quercetin isomers, and 3--{2--{6--[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Kaempferide. The discovery of bioactive compounds could provide references for the quality control and novel pharmaceuticals development of GRE. The present work proposes a feasible chromatogram-bioactivity correlation based approach to discover the compounds and define their bioactivities for the complex multi-component systems.
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http://dx.doi.org/10.3390/molecules23051071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102599PMC
May 2018

Practical gigahertz quantum key distribution robust against channel disturbance.

Opt Lett 2018 May;43(9):2030-2033

Quantum key distribution (QKD) provides an attractive solution for secure communication. However, channel disturbance severely limits its application when a QKD system is transferred from the laboratory to the field. Here a high-speed Faraday-Sagnac-Michelson QKD system is proposed that can automatically compensate for the channel polarization disturbance, which largely avoids the intermittency limitations of environment mutation. Over a 50 km fiber channel with 30 Hz polarization scrambling, the practicality of this phase-coding QKD system was characterized with an interference fringe visibility of 99.35% over 24 h and a stable secure key rate of 306 k bits/s over seven days without active polarization alignment.
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http://dx.doi.org/10.1364/OL.43.002030DOI Listing
May 2018

Preparative expression and purification of a nacreous protein N16 and testing its effect on osteoporosis rat model.

Int J Biol Macromol 2018 May 10;111:440-445. Epub 2018 Jan 10.

Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed TCM, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China. Electronic address:

N16, a nacreous protein isolated from Pinctada martensii, is related to nacreous layer formation. Our previous study indicated that N16 showed dual regulatory effects by inducing osteoblast biomineralization as well as inhibiting osteoclast formation. In order to obtain large quantity of N16 for animal experiment and clinical trial, a fermentation and preparative purification method was established. The N16 cDNA was cloned to a BL21(DE3)plysE-pET32a vector and grown in a 20 L fermenter. The medium, temperature, pH and dissolved oxygen (DO) were optimized. N16 was expressed in inclusion bodies. It was denatured and refolded in 8 M urea buffer and purified to 97% purity by passing through a gel filtration column. The glucocorticoid induced osteoporosis (GIO) rat model was used to investigate the anti-osteoporosis activity of N16 in vivo. Results showed that the decrease of the bone mineral density (BMD) and the ultimate load was significantly relieved after N16 treatment. N16 displayed dual regulatory effects by promoting osteogenesis as well as inhibiting bone resorption in vivo. Our work will contribute to further clinical studies on N16 for osteoporosis treatment.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.01.054DOI Listing
May 2018
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