Publications by authors named "Yong Cheng"

438 Publications

Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression.

Nat Genet 2021 May 6. Epub 2021 May 6.

Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Pinpointing functional noncoding DNA sequences and defining their contributions to health-related traits is a major challenge for modern genetics. We developed a high-throughput framework to map noncoding DNA functions with single-nucleotide resolution in four loci that control erythroid fetal hemoglobin (HbF) expression, a genetically determined trait that modifies sickle cell disease (SCD) phenotypes. Specifically, we used the adenine base editor ABEmax to introduce 10,156 separate A•T to G•C conversions in 307 predicted regulatory elements and quantified the effects on erythroid HbF expression. We identified numerous regulatory elements, defined their epigenomic structures and linked them to low-frequency variants associated with HbF expression in an SCD cohort. Targeting a newly discovered γ-globin gene repressor element in SCD donor CD34 hematopoietic progenitors raised HbF levels in the erythroid progeny, inhibiting hypoxia-induced sickling. Our findings reveal previously unappreciated genetic complexities of HbF regulation and provide potentially therapeutic insights into SCD.
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http://dx.doi.org/10.1038/s41588-021-00861-8DOI Listing
May 2021

Nanoparticle-based applications for cervical cancer treatment in drug delivery, gene editing, and therapeutic cancer vaccines.

Wiley Interdiscip Rev Nanomed Nanobiotechnol 2021 May 4:e1718. Epub 2021 May 4.

Department of Radiation Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Cervical cancer is a leading cause of gynecological tumor related deaths worldwide. The applications of conventional approaches such as chemoradiotherapy and surgery are restricted due to their side effects and drug resistances. Although immune checkpoint inhibitors (ICIs) have emerged as novel choices, their clinical response rates are rather limited. To date there is a lack of effective treatment regimens for patients with metastatic or recurrent cervical cancer. Recently nanomaterials like liposomes, dendrimers, and polymers are considered as promising delivery carriers with advantages of tumor-specific administration, reduced toxicity, and improved biocompatibility. Here, we review the applications of nanoparticles in the fields of drug delivery, CRISPR based genome-editing and therapeutic vaccines in cervical cancer treatment. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease.
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http://dx.doi.org/10.1002/wnan.1718DOI Listing
May 2021

A highly promising high-nickel low-cobalt lithium layered oxide cathode material for high-performance lithium-ion batteries.

J Colloid Interface Sci 2021 Apr 8;597:334-344. Epub 2021 Apr 8.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address:

Reducing cobalt dependency has attracted great interest for lithium batteries manufacturing due to limited cobalt resources and high prices. A highly promising LiNiCoMnO (NCM60535) high-nickel low cobalt lithium layered oxide cathode material is successfully prepared by systematically examining the two key synthesis conditions of pH and annealing temperature. The obtained materials exhibit a uniform size distribution, good spherical morphology, clear structure, and homogeneous element distribution. NCM60535 shows competitive electrochemical properties: when compared with the LiNiCoMnO, with a higher output specific capacity and cycling stability at 4.3 V low voltage; when compared with the LiNiCoMnO, with a comparable discharge capacity but relatively poor cycling stability at 4.5 V high voltage. A new type of electrolyte that combines high lithium salt concentration, EC-free solvent system, and VC and LiPOF functional additives is designed and greatly improves the electrochemical properties of the material under high voltage. Moreover, it also delivers superior electrochemical properties in high voltage lithium full battery (270 Wh Kg). And we suggest that NCM60535 is expected to become a substitute for the currently widely commercialized LiNiCoMnO (NCM333), LiNiCoMnO (NCM523), LiNiCoMnO (NCM622), and LiNiCoMnO (NCM811) due to its relatively low production cost and competitive electrochemical properties.
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http://dx.doi.org/10.1016/j.jcis.2021.04.008DOI Listing
April 2021

Polysaccharide conjugates from Chin brick tea (Camellia sinensis) improve the physicochemical stability and bioaccessibility of β-carotene in oil-in-water nanoemulsions.

Food Chem 2021 Apr 1;357:129714. Epub 2021 Apr 1.

Zhejiang Skyherb Biotechnology Inc., Anji, Zhejiang 313300, China.

A natural antioxidant emulsifier, tea polysaccharide conjugate (TPC), was isolated from Chin brick tea. The impact of TPC on β-carotene stability and bioaccessibility in oil-in-water nanoemulsions was assessed. TPC exhibited strong antioxidant activity and could be used to fabricate stable nanoemulsions (d < 140 nm). The extent of lipid digestion was considerably lower for lipid droplets coated by TPC (68%) than Tween 80 (94%) or whey protein isolate (WPI) (89%), probably because TPC formed interfacial layers that hindered the access of lipases to lipids. The chemical stability of β-carotene in TPC-nanoemulsions was markedly higher than in those formulated with Tween 80 or WPI due to the high antioxidant activity of TPC. The bioaccessibility of β-carotene (20-30%) was independent of emulsifier type. TPC from Chin brick tea can therefore be used as a dual-purpose functional ingredient in emulsified foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.129714DOI Listing
April 2021

Correction: 1,2,4-Trimethoxybenzene selectively inhibits NLRP3 inflammasome activation and attenuates experimental autoimmune encephalomyelitis.

Acta Pharmacol Sin 2021 Apr 13. Epub 2021 Apr 13.

Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

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http://dx.doi.org/10.1038/s41401-021-00670-zDOI Listing
April 2021

Exosome Transplantation From Patients With Schizophrenia Causes Schizophrenia-Relevant Behaviors in Mice: An Integrative Multi-omics Data Analysis.

Schizophr Bull 2021 Apr 10. Epub 2021 Apr 10.

Key Laboratory of Ethnomedicine of Ministry of Education, Center on Translational Neuroscience, School of Pharmacy, Minzu University of China, Beijing, China.

Exosomes are involved in the pathophysiology of neuropsychiatric diseases, but the role of exosomes in schizophrenia (SCZ) is unclear. Here, we demonstrate that transplantation of serum exosomes from SCZ patients into mice caused behavioral abnormalities such as deficits in prepulse inhibition and sociability, hyperactivity, and anxiogenesis. A comparative bioinformatics analysis suggested shared and distinct differentially expressed genes (DEGs) and enriched molecular pathways in the brains of SCZ exosome-recipient mice, methylazoxymethanol acetate-treated rats, and SCZ patients, which correlates evidence of altered prefrontal-hippocampal functional coherence in SCZ. A large proportion of SCZ-relevant DEGs in the exosome-recipient mice were targets of DE exosomal miRNAs in SCZ patients. Furthermore, we identified 20 hub genes for SCZ risk genes, including BDNF and NRG1, which were DE miRNA targets in SCZ. Collectively, our study suggests that SCZ exosome transplantation caused SCZ-relevant behaviors in mice, and epigenetic regulation may contribute to the phenotypes in the SCZ exosome-recipient mice. Our results may provide a potential animal model and novel therapeutic targets for SCZ.
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http://dx.doi.org/10.1093/schbul/sbab039DOI Listing
April 2021

Systemic conbercept pharmacokinetics and VEGF pharmacodynamics following intravitreal injections of conbercept in patients with retinopathy of prematurity.

Br J Ophthalmol 2021 Apr 9. Epub 2021 Apr 9.

Department of Ophthalmology, Peking University People's Hospital, Eye diseases and Optometry Institute, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Clinical Centre of Optometry, College of Optometry, Peking University Health science center, Beijing, China

Background: Data on serum vascular endothelial growth factor (VEGF) and drug levels in patients with retinopathy of prematurity (ROP) following intravitreal injections of conbercept (IVC) are lacking.

Methods: Multicentre, prospective, non-randomised study of patients with aggressive posterior retinopathy of prematurity (APROP) or type 1 ROP who had not received other treatment. All infants received therapy in both eyes plus intravitreal IVC 0.25 mg/0.025 mL in one eye and had at least 6 months of follow-up. Blood samples were collected before and 1 week and 4 weeks after IVC. The main outcome measures were serum conbercept and VEGF concentrations.

Results: Forty infants with APROP or type 1 ROP were enrolled. The mean serum VEGF at baseline and 1 week and 4 weeks after a total of 0.25 mg of IVC was 953.35±311.90 pg/mL, 303.46±181.89 pg/mL and 883.12±303.89 pg/mL, respectively. Serum VEGF 1 week after IVC was significantly lower (p0.05) than baseline, and at 4 weeks after IVC, it was significantly higher (p0.05) than at 1 week. There was no significant difference (p0.05) between baseline and 4 weeks. Serum conbercept was below the limit of quantitation (BLOQ) at baseline and 4 weeks and was 19.81±7.60 ng/mL at 1 week.

Conclusion: Serum VEGF 1 week after IVC was significantly lower than baseline but returned to baseline at 4 weeks. Serum conbercept increased at 1 week and was BLOQ at 4 weeks.
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http://dx.doi.org/10.1136/bjophthalmol-2021-319131DOI Listing
April 2021

Circ_0091579 enhances the malignancy of hepatocellular carcinoma via miR-1287/PDK2 axis.

Open Life Sci 2021 25;16(1):69-83. Epub 2021 Jan 25.

Department of General Surgery, Ankang People's Hospital of Shanxi Province, Ankang, 6-1-3302, Shifu Courtyard, High-Tech Zone, Ankang 725000, Shanxi Province, China.

Several articles have indicated that circular RNAs are involved in pathogenesis of human cancers. Nevertheless, the role of circ_0091579 in hepatocellular carcinoma (HCC) progression remains to be revealed. Quantitative reverse transcriptase polymerase chain reaction was carried out to examine the expression of circ_0091579 and miR-1287. The proliferation of HCC cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry was performed to analyze cell cycle progression and apoptosis. Western blot assay was conducted to detect the protein expression of CyclinD1, Cleaved caspase3, and pyruvate dehydrogenase kinase 2 (PDK2). Cell glycolysis was evaluated by measuring the uptake of glucose, the production of lactate, and extracellular acidification rate. The target relationship between miR-1287 and circ_0091579 or PDK2 was verified by dual-luciferase reporter assay, RNA immunoprecipitation assay, and RNA-pull down assay. The enrichment of circ_0091579 was enhanced in HCC tissues ( = 77) and four HCC cell lines (HB611, Huh-7, MHCC97, and SNU423) compared with adjacent non-tumor tissues ( = 77) and normal human liver cell line THLE-2. Circ_0091579 mediated the promotion of proliferation and glycolysis and the suppression of apoptosis of HCC cells. MiR-1287 was a direct target of circ_0091579 in HCC cells. MiR-1287 knockdown reversed the effects caused by circ_0091579 interference on the functions of HCC cells. PDK2 could bind to miR-1287 in HCC cells. Circ_0091579 upregulated the enrichment of PDK2 by acting as a sponge of miR-1287 in HCC cells. The influence caused by circ_0091579 intervention on HCC cells was attenuated by overexpression of PDK2. Circ_0091579 interference impeded the progression of HCC . Circ_0091579 deteriorated HCC by promoting the proliferation and glycolytic metabolism and suppressing the apoptosis of HCC cells via miR-1287/PDK2 axis.
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http://dx.doi.org/10.1515/biol-2021-0009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874672PMC
January 2021

Perfluorododecanoic acid delays Leydig cell regeneration from stem cells in adult rats.

Food Chem Toxicol 2021 May 25;151:112152. Epub 2021 Mar 25.

Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou, Zhejiang, 325027, China. Electronic address:

Perfluorododecanoic acid (PFDoA) is an endocrine-damaging compound in contaminated food and water. However, the potential role and underlying mechanism of PFDoA in Leydig cell regeneration from stem Leydig cells remain unclear. The current study aims to investigate the effect of PFDoA on the regeneration of Leydig cells in the testis of rats treated with ethylene dimethane sulfonate (EDS). PFDoA (0, 5 or 10 mg/kg/day) was gavaged to adult Sprague-Dawley male rats for 8 days, and 75 mg/kg EDS was intraperitoneally injected to eliminate Leydig cells to initiate its regeneration from day 21-56 after EDS. The serum testosterone levels in the 5 and 10 mg/kg/day PFDoA groups were significantly reduced at day 21 after EDS and the levels of serum luteinizing hormone and follicle-stimulating hormone were significantly decreased in the 10 mg/kg/day PFDoA groups at day 56 after EDS. PFDoA significantly reduced Leydig cell number and proliferation at a dose of 10 mg/kg at days 21 and 56 after EDS. PFDoA significantly down-regulated the expression of Leydig cell-specific genes (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1 and Cyp17a1) and their proteins at both doses at days 21 and 56 after EDS. PFDoA significantly down-regulated the gene expression of Sertoli cells (Fshr, Dhh, and Sox9) at 5 mg/kg or higher at days 21 and 56 after EDS. In addition, we found that PFDoA significantly inhibited EdU incorporation into putative stem Leydig cells and their differentiation into the Leydig cell lineage in vitro. In conclusion, short-term PFDoA exposure in adulthood delayed the regeneration of Leydig cells by preventing Leydig cells from stem cells via multiple mechanisms.
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http://dx.doi.org/10.1016/j.fct.2021.112152DOI Listing
May 2021

Real-world outcomes of two-year Conbercept therapy for diabetic macular edema.

Int J Ophthalmol 2021 18;14(3):416-422. Epub 2021 Mar 18.

Department of Ophthalmology, People's Hospital, Peking University, Beijing 100044, China.

Aim: To evaluate the two-year outcomes of patients treated for diabetic macular edema (DME) with intravitreal Conbercept (IVC).

Methods: The clinical data of 30 DME patients (36 eyes) were retrospectively reviewed. The patients were treated with IVC for 3mo. Additional IVC was given at subsequent monthly visits, if needed (3+PRN). The patients were followed up for 24mo.

Results: The best-corrected visual acuity (BCVA) at 24mo significantly increased (66.7±15.3 letters) in comparison with the baseline (54.4±15.4 letters, <0.0001). The mean improvement in BCVA was 11.0±2.9 letters. At 24mo, 44.1% of the eyes surveyed gained ≥15 letters, 52.9% of the eyes gained ≥10 letters, and 70.6% of the eyes gained ≥5 letters. No vision loss was noted in 96.8% of the eyes, and 5.9% of the eyes lost ≥5 letters, but ≤10 letters. The central retinal thickness (CRT) at 24mo was significantly reduced (277.1±122.9 µm) in comparison with the baseline (510.9±186.1 µm, <0.0001). At 24mo, 43.3% of the patients had a CRT ≤250 µm. The mean number of injections over 24mo was 10.6±2.0. No severe eye or systemic adverse events related to either the drug or injection were noted.

Conclusion: IVC is safe and effective for the treatment of DME.
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http://dx.doi.org/10.18240/ijo.2021.03.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930533PMC
March 2021

Traditional Chinese Medicine Decoction Combined With Antipsychotic for Chronic Schizophrenia Treatment: A Systematic Review and Meta-analysis.

Front Pharmacol 2020 20;11:616088. Epub 2021 Jan 20.

Key Laboratory of Ethnomedicine of Ministry of Education, Center on Translational Neuroscience, School of Pharmacy, Minzu University of China, Beijing, China.

Despite several studies suggesting the effectiveness of traditional Chinese medicine (TCM) in schizophrenia, there is still a lack of systematic summary and analysis on the role of TCM as adjuvant therapy in chronic schizophrenia. For this purpose, we conducted a meta-analysis to study the efficacy of TCM as an adjuvant combined with antipsychotics in the treatment of chronic schizophrenia. Until April 2020, based on the review of six electronic databases, eight articles were selected. The articles compared TCM decoction assisted antipsychotic therapies with an antipsychotic alone in the treatment of chronic schizophrenia by analyzing a total of 810 cases. The results showed that TCM combined with antipsychotics have beneficial effects on the Positive and Negative Syndrome Scale (PANSS), including the changes in total score, negative score, and the clinical effects evaluated by the PANSS scale. Subgroup analysis showed that the effects of auxiliary TCM with different efficacy on the positive and psychopathological scores were significantly different. It was found that adjuvant treatment with TCM can reduce some side effects and improve the patient's living conditions in the evaluation of the Schizophrenia Quality Of Life Scale (SQLS). Many studies have proved that TCM is safe and well-tolerated. Although the difficulties of using limited TCM remains to be generalized, it still has great potential in the adjuvant treatment of chronic schizophrenia.
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http://dx.doi.org/10.3389/fphar.2020.616088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942273PMC
January 2021

The Comprehensive Analyses of Genomic Variations and Assessment of TMB and PD-L1 Expression in Chinese Lung Adenosquamous Carcinoma.

Front Genet 2020 17;11:609405. Epub 2021 Feb 17.

Department of Respiratory Diseases, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

The poor prognosis and fewer treatment option is a current clinical challenge for patients with lung adenosquamous carcinoma (ASC). The previous studies reported that tumor mutational burden (TMB, numbers of mutation per Megabase) is a predictor of clinical response in trials of multiple cancer types, while fewer studies assessed the relationship between TMB level and clinical features and outcomes of lung ASC. Herein, the present study enrolled Chinese patients with lung ASC. DNA was extracted from formalin-fixed paraffin-embedded tumor samples and subjected to next generation sequencing (NGS), and the 457 cancer related genes were evaluated. The results demonstrated that 95 unique genes with somatic variations were identified in the enrolled patients. The top three of high frequency gene mutations were with rates of 62% (13 cases), 48% (10 cases), and 14% (3 cases), respectively. We identified TMB value was significantly correlated with pathological stages ( < 0.05) and invasion of lymph node ( < 0.05). However, TMB value was not significantly correlated to other clinicopathologic indexes, for examples, age, sex, smoking history, tumor size, as well as and mutations in lung ASC. Moreover, TMB value was associated with the overall survival ( < 0.01), but not with the relapse-free survival ( = 0.23). In conclusion, this study indicated that lung ASC with high TMB might be associated with the invasion of lymph node and short overall survival. Immunotherapy might be a promising treatment option for lung ASC patients with high TMB.
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http://dx.doi.org/10.3389/fgene.2020.609405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925901PMC
February 2021

M2 macrophage-derived exosomal microRNAs inhibit cell migration and invasion in gliomas through PI3K/AKT/mTOR signaling pathway.

J Transl Med 2021 03 6;19(1):99. Epub 2021 Mar 6.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, No 169 Donghu Road, Wuhan, 430071, Hubei, China.

Background: Glioma, the most common primary brain tumor, account Preparing figures for 30 to 40% of all intracranial tumors. Herein, we aimed to study the effects of M2 macrophage-derived exosomal microRNAs (miRNAs) on glioma cells.

Methods: First, we identified seven differentially expressed miRNAs in infiltrating macrophages and detected the expression of these seven miRNAs in M2 macrophages. We then selected hsa-miR-15a-5p (miR-15a) and hsa-miR-92a-3p (miR-92a) for follow-up studies, and confirmed that miR-15a and miR-92a were under-expressed in M2 macrophage exosomes. Subsequently, we demonstrated that M2 macrophage-derived exosomes promoted migration and invasion of glioma cells, while exosomal miR-15a and miR-92a had the opposite effects on glioma cells. Next, we performed the target gene prediction in four databases and conducted target gene validation by qRT-PCR, western blot and dual luciferase reporter gene assays.

Results: The results revealed that miR-15a and miR-92a were bound to CCND1 and RAP1B, respectively. Western blot assays demonstrated that interference with the expression of CCND1 or RAP1B reduced the phosphorylation level of AKT and mTOR, indicating that both CCND1 and RAP1B can activate the PI3K/AKT/mTOR signaling pathway.

Conclusion: Collectively, these findings indicate that M2 macrophage-derived exosomal miR-15a and miR-92a inhibit cell migration and invasion of glioma cells through PI3K/AKT/mTOR signaling pathway.
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http://dx.doi.org/10.1186/s12967-021-02766-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937290PMC
March 2021

Selective and sensitive photoelectrochemical aptasensor for streptomycin detection based on BiVOBr/TiC nanohybrids.

J Hazard Mater 2021 Feb 25;414:125539. Epub 2021 Feb 25.

Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, OE, School of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address:

Sensitive detection of streptomycin (STR) has attracted increasing attention worldwide because of the relationship between food security and human health. In this paper, BiVOBr/TiC nanohybrids were obtained by one-pot solvent hydrothermal method. It was modified on ITO electrode, and STR aptamer was acted as the recognition element. With excellent photoelectrochemical (PEC) performance of BiVOBr/TiC nanohybrids, an "on-off-on" PEC aptasensor for STR detection was effectively developed. Compared with pure BiVOBr, the photocurrent intensity of as-prepared BiVOBr/TiC nanohybrids was about 9 times higher, which ascribed to the highly conductive of TiC, driving the photogenerated electrons transferred to the ITO electrode rapidly, so that the recombination of photogenerated electron and hole pairs was inhibited viably. Furthermore, the constructed "on-off-on" PEC aptasensor accomplished STR detection with high sensitivity, excellent specificity and distinguished repeatability in honey. The photocurrent increased with the increment of STR concentration with the linear range from1 nM to 1000 nM, and the detection limit of 0.3 nM (S/N = 3). Compared with the national standard method (SN/T 1925-2007), the as-constructed PEC sensor showed the consistent results.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125539DOI Listing
February 2021

Incidence of psychological illness after coronavirus outbreak: a meta-analysis study.

J Epidemiol Community Health 2021 Feb 25. Epub 2021 Feb 25.

Key Laboratory of Ethnomedicine of Ministry of Education, Center on Translational Neuroscience, School of Pharmacy, Minzu University of China, Beijing, China

Background: The COVID-19 has had an enormous impact worldwide and is still spreading. Globally confirmed infections have surpassed 41.1 million, of which more than 1 million resulted in deaths. Considering the relationship between public health disasters and emotional disorders, it is essential to examine psychological well-being related to this pandemic.

Method: We performed a systematic search on psychological problems from PubMed to 10 October 2020, and conducted a meta-analysis using Comprehensive Meta-Analysis V.3 software.

Results: The results showed a 19.4% and 26.8% pooled incidence for depression and post-traumatic stress disorder (PTSD), respectively, during the SARS and Middle East Respiratory Syndrome (MERS)-related coronavirus outbreaks. However, overall prevalence of depression was somewhat higher at 27.0% during the COVID-19 period. The pooled incidence of PTSD during COVID-19 compared with SARS and MERS outbreaks, was lower, at 16.4%.

Conclusion: The results suggest that there are shared and distinct psychological responses following SARS, MERS and COVID-19, and show pessimistic estimates of a wide range of potentially upcoming psychological problems.
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http://dx.doi.org/10.1136/jech-2020-215927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908057PMC
February 2021

1,2,4-Trimethoxybenzene selectively inhibits NLRP3 inflammasome activation and attenuates experimental autoimmune encephalomyelitis.

Acta Pharmacol Sin 2021 Feb 24. Epub 2021 Feb 24.

Center on Translational Neuroscience, College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.

NOD-like receptor (NLR) family pyrin domain-containing-3 (NLRP3) inflammasome is implicated in inflammation-associated diseases such as multiple sclerosis, Parkinson's disease, and stroke. Targeting the NLRP3 inflammasome is beneficial to these diseases, but few NLRP3 inflammasome-selective inhibitors are identified to date. Essential oils (EOs) are liquid mixtures of volatile and low molecular-weight organic compounds extracted from aromatic plants, which show various pharmacological activities, including antibacterial, antifungal, antiviral, antioxidant, and anti-inflammatory properties. In this study we screened active ingredients from essential oils, and identified 1,2,4-trimethoxybenzene (1,2,4-TTB) as a selective NLRP3 inflammasome inhibitor. We showed that 1,2,4-TTB (1 mM) markedly suppressed nigericin- or ATP-induced NLRP3 inflammasome activation, thus decreased caspase-1 activation and IL-1β secretion in immortalized murine bone marrow-derived macrophages (iBMDMs) and in primary mouse microglia. Moreover, 1,2,4-TTB specifically inhibited the activation of NLRP3 inflammasome without affecting absent in melanoma 2 (AIM2) inflammasome activation. We further demonstrated that 1,2,4-TTB inhibited oligomerization of the apoptosis-associated speck-like protein containing a CARD (ASC) and protein-protein interaction between NLRP3 and ASC, thus blocking NLRP3 inflammasome assembly in iBMDMs and in primary mouse macrophages. In mice with experimental autoimmune encephalomyelitis (EAE), administration of 1,2,4-TTB (200 mg · kg · d, i.g. for 17 days) significantly ameliorated EAE progression and demyelination. In conclusion, our results demonstrate that 1,2,4-TTB is an NLRP3 inflammasome inhibitor and attenuates the clinical symptom and inflammation of EAE, suggesting that 1,2,4-TTB is a potential candidate compound for treating NLRP3 inflammasome-driven diseases, such as multiple sclerosis.
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http://dx.doi.org/10.1038/s41401-021-00613-8DOI Listing
February 2021

An SiO anode strengthened by the self-catalytic growth of carbon nanotubes.

Nanoscale 2021 Feb 10;13(6):3808-3816. Epub 2021 Feb 10.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun, 130022, China.

Modification using carbon nanotubes (CNTs) is one of the most important strategies to boost the performance of materials in various applications, among which the CNT-modified silicon-based anodes have gained considerable attention in lithium-ion batteries (LIBs) due to their improved conductivity and cycle stability. However, the realization of a close-knit CNT coating on silicon (Si) through an efficient and cost-effective approach remains challenging. Herein, a new in situ self-catalytic method by acetylene treatment is presented, in which, CNTs can be directly grown and knitted on the SiO particles to construct a conductive additive-free SiO@CNT anode. The in situ grown CNTs can not only enhance electric conductivity and alleviate the volume effect of SiO effectively, but also mitigate the electrolyte decomposition with improved coulombic efficiency. As a result, an extremely high capacity of 1012 mA h g, long lifespan over 500 cycles at a current density of 2 A g as well as a good performance in full LIBs with a working potential of about 3.4 V (vs. nickel-rich cathode) were obtained. The rationally constructed SiO@CNTs with easy synthesis and high throughput will hopefully promote LIBs with energy density above 300 W h kg. This study opens a new avenue to prepare CNT-decorated functional materials and brings the SiO-based anode one step closer to practical applications.
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http://dx.doi.org/10.1039/d0nr08297jDOI Listing
February 2021

A method for evaluation of patient-specific lean body mass from limited-coverage CT images and its application in PERCIST: comparison with predictive equation.

EJNMMI Phys 2021 Feb 8;8(1):12. Epub 2021 Feb 8.

Department of Nuclear Medicine and PET/CT-MRI Center, The First Affiliated Hospital of Jinan University, No. 613 West Huangpu Road, Guangzhou, 510630, China.

Background: Standardized uptake value (SUV) normalized by lean body mass ([LBM] SUL) is recommended as metric by PERCIST 1.0. The James predictive equation (PE) is a frequently used formula for LBM estimation, but may cause substantial error for an individual. The purpose of this study was to introduce a novel and reliable method for estimating LBM by limited-coverage (LC) CT images from PET/CT examinations and test its validity, then to analyse whether SUV normalised by LC-based LBM could change the PERCIST 1.0 response classifications, based on LBM estimated by the James PE.

Methods: First, 199 patients who received whole-body PET/CT examinations were retrospectively retrieved. A patient-specific LBM equation was developed based on the relationship between LC fat volumes (FV) and whole-body fat mass (FM). This equation was cross-validated with an independent sample of 97 patients who also received whole-body PET/CT examinations. Its results were compared with the measurement of LBM from whole-body CT (reference standard) and the results of the James PE. Then, 241 patients with solid tumours who underwent PET/CT examinations before and after treatment were retrospectively retrieved. The treatment responses were evaluated according to the PE-based and LC-based PERCIST 1.0. Concordance between them was assessed using Cohen's κ coefficient and Wilcoxon's signed-ranks test. The impact of differing LBM algorithms on PERCIST 1.0 classification was evaluated.

Results: The FV were significantly correlated with the FM (r=0.977). Furthermore, the results of LBM measurement evaluated with LC images were much closer to the reference standard than those obtained by the James PE. The PE-based and LC-based PERCIST 1.0 classifications were discordant in 27 patients (11.2%; κ = 0.823, P=0.837). These discordant patients' percentage changes of peak SUL (SUL) were all in the interval above or below 10% from the threshold (±30%), accounting for 43.5% (27/62) of total patients in this region. The degree of variability is related to changes in LBM before and after treatment.

Conclusions: LBM algorithm-dependent variability in PERCIST 1.0 classification is a notable issue. SUV normalised by LC-based LBM could change PERCIST 1.0 response classifications based on LBM estimated by the James PE, especially for patients with a percentage variation of SUL close to the threshold.
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http://dx.doi.org/10.1186/s40658-021-00358-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870732PMC
February 2021

The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation.

Sci Rep 2021 Feb 4;11(1):3101. Epub 2021 Feb 4.

Key Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Center On Translational Neuroscience, College of Life and Environmental Sciences, Minzu University of China, No. 27, South Street of Zhongguancun, Haidian District, Beijing, 100081, China.

Foot electrical stimulation (FES) has been considered as a classic stressor that can disturb homeostasis. Acute anemia was observed in the model induced by FES. The aim of this study was to explore the role of inflammatory cytokines underlying the acute anemia and gastrointestinal (GI) mucosal injury in the FES. Twenty-four male Kunming mice (20 ± 2 g) were randomly divided into control group and experimental group. The mice were placed in a footshock chamber that can generate 0.5 mA electrical impulse periodically for 0.5 h. After the process, red blood cell count, hemoglobin concentration and hematocrit, the levels of corticotropin releasing hormone (CRH) in serum and hypothalamus, and adrenocorticotropic hormone (ACTH) in serum and pituitary were detected separately. In addition, we investigated the expressions of inflammatory cytokines (IL-1, IL-6, TNF-α, iNOS, and IL-10) in the hypothalamus and duodenum by Polymerase Chain Reaction (PCR). Results showed that this FES model induced anemia, increased CRH and ACTH activity in the serum after the FES. Moreover, the expressions of IL-1β, IL-6, TNF-α, and iNOS were significantly increased following the process, while IL-10 was not activated. These findings suggest that anemia, the inflammatory cytokines in the hypothalamus and duodenum of the mice in the model induced by FES is closely related to GI mucosal injury/bleeding. Taken together, these results underscore the importance of anemia, GI mucosal injury/bleeding and stress, future studies would be needed to translate these findings into the benefit of affected patients.
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http://dx.doi.org/10.1038/s41598-021-82604-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862408PMC
February 2021

MIL-101(Cr) with incorporated polypyridine zinc complexes for efficient degradation of a nerve agent simulant: spatial isolation of active sites promoting catalysis.

Dalton Trans 2021 Feb 1;50(6):1995-2000. Epub 2021 Feb 1.

The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials (State Key Laboratory Cultivation Base), College of Chemistry and Materials Science Anhui Normal University, Wuhu, Anhui 241002, P.R. China.

Development of an efficient catalyst for degradation of organophosphorus toxicants is highly desirable. Herein, an MIL-101(Cr)LZn catalyst was fabricated by incorporating polypyridine zinc complexes into a MOF to achieve the spatial isolation of active sites. Compared with a terpyridine zinc complex without an MIL-101 support, this catalyst was highly active for detoxification of diethyl-4-nitrophenylphosphate.
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http://dx.doi.org/10.1039/d0dt04048gDOI Listing
February 2021

Dynamic CTCF binding directly mediates interactions among cis-regulatory elements essential for hematopoiesis.

Blood 2021 Mar;137(10):1327-1339

Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN.

While constitutive CCCTC-binding factor (CTCF)-binding sites are needed to maintain relatively invariant chromatin structures, such as topologically associating domains, the precise roles of CTCF to control cell-type-specific transcriptional regulation remain poorly explored. We examined CTCF occupancy in different types of primary blood cells derived from the same donor to elucidate a new role for CTCF in gene regulation during blood cell development. We identified dynamic, cell-type-specific binding sites for CTCF that colocalize with lineage-specific transcription factors. These dynamic sites are enriched for single-nucleotide polymorphisms that are associated with blood cell traits in different linages, and they coincide with the key regulatory elements governing hematopoiesis. CRISPR-Cas9-based perturbation experiments demonstrated that these dynamic CTCF-binding sites play a critical role in red blood cell development. Furthermore, precise deletion of CTCF-binding motifs in dynamic sites abolished interactions of erythroid genes, such as RBM38, with their associated enhancers and led to abnormal erythropoiesis. These results suggest a novel, cell-type-specific function for CTCF in which it may serve to facilitate interaction of distal regulatory emblements with target promoters. Our study of the dynamic, cell-type-specific binding and function of CTCF provides new insights into transcriptional regulation during hematopoiesis.
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http://dx.doi.org/10.1182/blood.2020005780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955410PMC
March 2021

CTLA-4 expression by B-1a B cells is essential for immune tolerance.

Nat Commun 2021 01 22;12(1):525. Epub 2021 Jan 22.

Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.

CTLA-4 is an important regulator of T-cell function. Here, we report that expression of this immune-regulator in mouse B-1a cells has a critical function in maintaining self-tolerance by regulating these early-developing B cells that express a repertoire enriched for auto-reactivity. Selective deletion of CTLA-4 from B cells results in mice that spontaneously develop autoantibodies, T follicular helper (Tfh) cells and germinal centers (GCs) in the spleen, and autoimmune pathology later in life. This impaired immune homeostasis results from B-1a cell dysfunction upon loss of CTLA-4. Therefore, CTLA-4-deficient B-1a cells up-regulate epigenetic and transcriptional activation programs and show increased self-replenishment. These activated cells further internalize surface IgM, differentiate into antigen-presenting cells and, when reconstituted in normal IgH-allotype congenic recipient mice, induce GCs and Tfh cells expressing a highly selected repertoire. These findings show that CTLA-4 regulation of B-1a cells is a crucial immune-regulatory mechanism.
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http://dx.doi.org/10.1038/s41467-020-20874-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822855PMC
January 2021

Peripheral blood neurotrophic factor levels in children with autism spectrum disorder: a meta-analysis.

Sci Rep 2021 Jan 8;11(1):15. Epub 2021 Jan 8.

Center On Translational Neuroscience, College of Life and Environmental Sciences, Minzu University of China, 27 South Zhongguancun Avenue, Zhongguancun South St, Haidian District, Beijing, 100081, China.

Increasing evidence suggests that abnormal regulation of neurotrophic factors is involved in the etiology and pathogenesis of Autism Spectrum Disorder (ASD). However, clinical data on neurotrophic factor levels in children with ASD were inconsistent. Therefore, we performed a systematic review of peripheral blood neurotrophic factors levels in children with ASD, and quantitatively summarized the clinical data of peripheral blood neurotrophic factors in ASD children and healthy controls. A systematic search of PubMed and Web of Science identified 31 studies with 2627 ASD children and 4418 healthy controls to be included in the meta-analysis. The results of random effect meta-analysis showed that the peripheral blood levels of brain-derived neurotrophic factor (Hedges' g = 0.302; 95% CI = 0.014 to 0.591; P = 0.040) , nerve growth factor (Hedges' g = 0.395; 95% CI = 0.104 to 0.686; P = 0.008) and vascular endothelial growth factor (VEGF) (Hedges' g = 0.097; 95% CI = 0.018 to 0.175; P = 0.016) in children with ASD were significantly higher than that of healthy controls, whereas blood neurotrophin-3 (Hedges' g =  - 0.795; 95% CI =  - 1.723 to 0.134; P = 0.093) and neurotrophin-4 (Hedges' g = 0.182; 95% CI =  - 0.285 to 0.650; P = 0.445) levels did not show significant differences between cases and controls. Taken together, these results clarified circulating neurotrophic factor profile in children with ASD, strengthening clinical evidence of neurotrophic factor aberrations in children with ASD.
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http://dx.doi.org/10.1038/s41598-020-79080-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794512PMC
January 2021

Oxidative stress marker aberrations in children with autism spectrum disorder: a systematic review and meta-analysis of 87 studies (N = 9109).

Transl Psychiatry 2021 01 5;11(1):15. Epub 2021 Jan 5.

Key Laboratory of Ethnomedicine of Ministry of Education, Center on Translational Neuroscience, College of Life and Environmental Sciences, Minzu University of China, Beijing, China.

There is increasing awareness that oxidative stress may be implicated in the pathophysiology of autism spectrum disorder (ASD). Here we aimed to investigate blood oxidative stress marker profile in ASD children by a meta-analysis. Two independent investigators systematically searched Web of Science, PubMed, and Cochrane Library and extracted data from 87 studies with 4928 ASD children and 4181 healthy control (HC) children. The meta-analysis showed that blood concentrations of oxidative glutathione (GSSG), malondialdehyde, homocysteine, S-adenosylhomocysteine, nitric oxide, and copper were higher in children with ASD than that of HC children. In contrast, blood reduced glutathione (GSH), total glutathione (tGSH), GSH/GSSG, tGSH/GSSG, methionine, cysteine, vitamin B9, vitamin D, vitamin B12, vitamin E, S-adenosylmethionine/S-adenosylhomocysteine, and calcium concentrations were significantly reduced in children with ASD relative to HC children. However, there were no significance differences between ASD children and HC children for the other 17 potential markers. Heterogeneities among studies were found for most markers, and meta-regressions indicated that age and publication year may influence the meta-analysis results. These results therefore clarified blood oxidative stress profile in children with ASD, strengthening clinical evidence of increased oxidative stress implicating in pathogenesis of ASD. Additionally, given the consistent and large effective size, glutathione metabolism biomarkers have the potential to inform early diagnosis of ASD.
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http://dx.doi.org/10.1038/s41398-020-01135-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791110PMC
January 2021

St. Jude Cloud: A Pediatric Cancer Genomic Data-Sharing Ecosystem.

Cancer Discov 2021 May 6;11(5):1082-1099. Epub 2021 Jan 6.

Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, Tennessee.

Effective data sharing is key to accelerating research to improve diagnostic precision, treatment efficacy, and long-term survival in pediatric cancer and other childhood catastrophic diseases. We present St. Jude Cloud (https://www.stjude.cloud), a cloud-based data-sharing ecosystem for accessing, analyzing, and visualizing genomic data from >10,000 pediatric patients with cancer and long-term survivors, and >800 pediatric sickle cell patients. Harmonized genomic data totaling 1.25 petabytes are freely available, including 12,104 whole genomes, 7,697 whole exomes, and 2,202 transcriptomes. The resource is expanding rapidly, with regular data uploads from St. Jude's prospective clinical genomics programs. Three interconnected apps within the ecosystem-Genomics Platform, Pediatric Cancer Knowledgebase, and Visualization Community-enable simultaneously performing advanced data analysis in the cloud and enhancing the Pediatric Cancer knowledgebase. We demonstrate the value of the ecosystem through use cases that classify 135 pediatric cancer subtypes by gene expression profiling and map mutational signatures across 35 pediatric cancer subtypes. SIGNIFICANCE: To advance research and treatment of pediatric cancer, we developed St. Jude Cloud, a data-sharing ecosystem for accessing >1.2 petabytes of raw genomic data from >10,000 pediatric patients and survivors, innovative analysis workflows, integrative multiomics visualizations, and a knowledgebase of published data contributed by the global pediatric cancer community..
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http://dx.doi.org/10.1158/2159-8290.CD-20-1230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102307PMC
May 2021

Microglial deletion and inhibition alleviate behavior of post-traumatic stress disorder in mice.

J Neuroinflammation 2021 Jan 5;18(1). Epub 2021 Jan 5.

The Brain Science Center, Beijing Institute of Basic Medical Sciences, No. 27 Taiping Road, Haidian District, Beijing, 100850, China.

Background: Alteration of immune status in the central nervous system (CNS) has been implicated in the development of post-traumatic stress disorder (PTSD). However, the nature of overall changes in brain immunocyte landscape in PTSD condition remains unclear.

Methods: We constructed a mouse PTSD model by electric foot-shocks followed by contextual reminders and verified the PTSD-related symptoms by behavior test (including contextual freezing test, open-field test, and elevated plus maze test). We examined the immunocyte panorama in the brains of the naïve or PTSD mice by using single-cell mass cytometry. Microglia number and morphological changes in the hippocampus, prefrontal cortex, and amygdala were analyzed by histopathological methods. The gene expression changes of those microglia were detected by quantitative real-time PCR. Genetic/pharmacological depletion of microglia or minocycline treatment before foot-shocks exposure was performed to study the role of microglia in PTSD development and progress.

Results: We found microglia are the major brain immune cells that respond to PTSD. The number of microglia and ratio of microglia to immunocytes was significantly increased on the fifth day of foot-shock exposure. Furthermore, morphological analysis and gene expression profiling revealed temporal patterns of microglial activation in the hippocampus of the PTSD brains. Importantly, we found that genetic/pharmacological depletion of microglia or minocycline treatment before foot-shock exposure alleviated PTSD-associated anxiety and contextual fear.

Conclusion: Our results demonstrated a critical role for microglial activation in PTSD development and a potential therapeutic strategy for the clinical treatment of PTSD in the form of microglial inhibition.
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http://dx.doi.org/10.1186/s12974-020-02069-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786489PMC
January 2021

Insight into the Coprecipitation-Controlled Crystallization Reaction for Preparing Lithium-Layered Oxide Cathodes.

ACS Appl Mater Interfaces 2021 Jan 2;13(1):717-726. Epub 2021 Jan 2.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

The nucleation and growth of spherical NiCoMn(OH) agglomerates using the hydroxide coprecipitation (HCP) method in the presence of ammonia is investigated through chemical equilibrium calculations and experiments. In the nucleation stage, the transition metal ions in the salt solution gradually complete the nucleation reaction in the diffusion process from pH 5.4 to 11 after dropping into the continuously stirred tank reactor, and then Me(NH) and Me(OH)(s) (Me: Ni, Co, and Mn) reach a dynamic precipitation dissolution equilibrium. In the growth stage, the concentration ratio of Me(NH) and OH (complexation and precipitation, ) in the solution has an important influence on obtaining high-quality materials, which is further confirmed using the first principles density functional theory calculations on surface energy and adsorption energy. Then, the HCP reaction could be divided into three parts through experiments: incomplete precipitation area ( > 10.1); time-dependent area ( = 0.1-10.1); and hard-to-control area ( <0.1). According to the optimal ratio ( = 3.4), a prediction formula for the optimal synthesis conditions of the materials is proposed ( = 0.7731 × ln( + 0.0312) + 11.6708, the optimal pH value () corresponds to different ammonia concentrations ()). The results obtained for the growth reaction mechanism and the prediction scheme would help the modification research of the materials and obtain the desired lithium-layered transition metal oxide cathode material with excellent performance in the shortest time.
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http://dx.doi.org/10.1021/acsami.0c19493DOI Listing
January 2021

Clinical and Cone-Beam Computed Tomography Features of Orthokeratinized Odontogenic Cysts and Odontogenic Keratocysts in the Mandible.

J Oral Maxillofac Surg 2020 Nov 26. Epub 2020 Nov 26.

Associate Professor and Vice President, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China; and, Department of Oral Radiology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address:

Purpose: The aim of the study was to compare the clinical and cone-beam computed tomography features of orthokeratinized odontogenic cysts (OOCs) and odontogenic keratocysts (OKCs) and to fully understand features of these 2 odontogenic cysts.

Materials And Methods: This retrospective cross-sectional study included patients with mandibular OOCs and OKCs. The predictor variables included age at the time of diagnosis, sex, anatomical location of the cysts, lesion size, cortical bone expansion rate, and presence of cortical bone destruction, impacted tooth, tooth displacement, and root resorption. The outcome variable was the type of cystic lesion. A 1-way analysis of variance test was used to analyze the differences among the cases of OOCs and OKCs. The specificity and sensitivity of the radiological features were calculated to differentiate OOCs from OKCs.

Result: The sample was composed of 12 patients with OOCs and 36 patients with OKCs. The mean ages of the patients with OOCs (30.50 ± 6.14 years) and OKCs (38.39 ± 19.44 years) were concentrated in the third decade. The cystic lesions occurring in areas II and III accounted for 66.67 and 52.78% of the OOC and OKC cases, respectively. The cortical bone expansion rate of the OOC was larger than that of the unilocular OKC (OOC, 2.20 ± 1.05; OKC, 1.48 ± 0.50; P < .05). The specificity and sensitivity of unilocular or multiocular cysts to differentiate OOC from OKC were 100%, 42%, 95% confidence interval of 0.1479 to 0.3892 (P < .05) and tooth displacement were 100%, 3%, 95% confidence interval of 0.1479 to 0.3892 (P < .05).

Conclusions: The results of this study suggest clinical and radiological features of OOCs and OKCs mostly overlap, but OOC has distinctive characteristics. Most cystic lesions of OOC are unilocular cysts, rarely accompanied by tooth displacement. The cortical bone expansion rate is larger than that of unilocular cysts of OKC.
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http://dx.doi.org/10.1016/j.joms.2020.11.021DOI Listing
November 2020

In Situ Growth of Lithiophilic MOF Layer Enabling Dendrite-free Lithium Deposition.

iScience 2020 Dec 27;23(12):101869. Epub 2020 Nov 27.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, CAS, Changchun 130022, China.

Lithium metal batteries have recently emerged as alternative energy storage systems beyond lithium-ion batteries. However, before this kind of batteries can become a viable technology, the critical issues of the Li anodes, like dendrites growth and low Coulombic efficiency (CE), need to be conquered. Herein, lithiophilic Cu-metal-organic framework thin layer (Cu-MOF TL) is grown on the surface of Cu foil by a facile immersion strategy to construct a multifunctional current collector. Profiting from the high electrical conductivity and unique porous structure, the Cu-MOF TL with high affinity to electrolyte can provide uniform nucleation sites and promote homogeneous Li flux, as a result, radically restrain the Li dendrite growth, leading to stable Li plating/striping behaviors. The modified current collector enables Li plating/stripping with a prominent CE (∼97.1%) and a stable lifetime (∼2500 h). Importantly, the synthesis method can be easily large-scale production in a series of organic solvents.
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http://dx.doi.org/10.1016/j.isci.2020.101869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733026PMC
December 2020

Amplified photocurrent signal for fabricating photoelectrochemical sulfadimethoxine aptasensor based on carbon nitride photosensitization with visible/near-infrared light responsive zinc phthalocyanine.

J Hazard Mater 2021 Mar 5;406:124749. Epub 2020 Dec 5.

Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China; Qingdao University of Science and Technology, Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao 266042, PR China. Electronic address:

Developing effective analytical method for sulfadimethoxine (SDM) detection is highly desirable and vitally crucial for protecting environment safety and human health. Herein, a highly selective and sensitive photoelectrochemical (PEC) aptasensor for accurate detection of SDM was proposed, which employed zinc phthalocyanine/graphitic carbon nitride (ZnPc/CN) nanocomposite as photosensitive material. The ZnPc/CN nanocomposite was constructed by modification of CN nanosheet with visible/near-infrared light responsive photosensitizer ZnPc. The introduction of ZnPc into CN exhibited amplified PEC response, which was 5.7 and 18.3 times than pure ZnPc and CN, attributed to the enhanced light harvesting ability and improved photoelectric conversion efficiency of such nanocomposite. By using ZnPc/CN and sulfadimethoxine (SDM) aptamer as PEC response material and specific probe, a PEC aptasensor was established for SDM detection. The aptamer was connected to the surface of chitosan/ZnPc/CN/ITO through the formation of phosphoramidate bonds between the amino group of the chitosan and phosphate group of the aptamer at 5' end. The fabricated aptasensor displayed good detection linearity of 0.1 ~ 300 nM and low detection limit of 0.03 nM (S/N = 3) under optimized conditions, and the potential applicability of the PEC aptasensor was confirmed by detecting SDM in milk powder samples.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124749DOI Listing
March 2021