Publications by authors named "Yong Chen"

3,050 Publications

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PeMetR-mediated sulphur assimilation is essential for virulence and patulin biosynthesis in Penicillium expansum.

Environ Microbiol 2021 Aug 4. Epub 2021 Aug 4.

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Penicillium expansum, as the causal agent of blue mould and a main producer of mycotoxin patulin, is a global concern for economic and food safety. To date, the nutritional requirements of the pathogen during infection and patulin biosynthesis are poorly understood. Here, we genetically characterized the role of the bZIP transcription factor PeMetR in sulphur metabolism, virulence and patulin biosynthesis of P. expansum. The PeMetR regulator is crucial for normal germination and growth on inorganic S-sources but dispensable for utilization of organic S-sources. Accordingly, it is involved in regulating the expression of genes in sulphur assimilation pathway rather than methionine metabolic processes. Disruption of PeMetR resulted in a complete loss of virulence on various fruits. Additionally, the mutant showed a remarkably reduced ability to produce patulin. Exogenous methionine could partially or completely rescue the impaired phenotypes of the mutant. Inactivation of the sulphur assimilation pathway genes, PesA, PesB, PesC, PesF, generated growth, virulence and patulin production defects similar to those of ΔPeMetR. Overall, our study provides evidence that PeMetR-mediated sulphur assimilation is essential for growth and infection, and shows for the first time that regulation of sulphur assimilation affects biosynthesis of an important mycotoxin patulin in P. expansum. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15704DOI Listing
August 2021

Environmental Contamination with SARS-CoV-2 in COVID-19 hospitals in Wuhan, China, 2020.

Environ Microbiol 2021 Aug 4. Epub 2021 Aug 4.

Chinese PLA Center for Disease Control & Prevention, Beijing, China.

Coronavirus disease 2019 (COVID-19) pandemic has caused high number of infections and deaths of healthcare workers globally. Distribution and possible transmission route of SARS-CoV-2 in hospital environment should be clarified. We herein collected 431 environmental (391 surface and 40 air) samples in the intensive care unit (ICU) and general wards (GWs) of three hospitals in Wuhan, China from February 21 to March 4, 2020, and detected SARS-CoV-2 RNA by real-time quantitative PCR. The viral positive rate in the contaminated areas was 17.8% (28/157) whereas there was no virus detected in the clean areas. Higher positive rate (22/59, 37.3%) was found in ICU than that in GWs (3/63, 4.8%). The surfaces of computer keyboards and mouse in the ICU were the most contaminated (8/10, 80.0%), followed by the ground (6/9, 66.7%) and outer glove (2/5, 40.0%). From 17 air samples in the contaminated areas, only one sample collected at a distance of around 30 cm from the patient was positive. Enhanced surface disinfection and hand hygiene effectively decontaminated the virus from the environment. This finding might help understand the transmission route and contamination risk of SARS-CoV-2 and evaluate the effectiveness of infection prevention and control measures in healthcare facilities. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15695DOI Listing
August 2021

Heat-induced gel properties and gastrointestinal digestive properties of egg white produced by hens fed with selenium-enriched yeast.

Food Chem 2021 Jul 27;366:130712. Epub 2021 Jul 27.

College of Food Sciences and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China. Electronic address:

The aim of this study was to investigate the effect of selenium enrichment on the gel properties and gastrointestinal digestive properties of egg white. Results of texture profile analysis, scanning electron microscopy and differential scanning calorimetry showed that the heat-induced gel of selenium-enriched egg white (EW-2) exhibited higher gel strength, smoother microstructure, and higher thermal denaturation temperature than ordinary egg white (EW-1), which might be due to the change of ovomucin and ovotransferrin content. The gastrointestinal digestive products of the EW-2 protein contained a higher proportion of small peptides and more free amino acids than those of EW-1. Results of oxygen radical absorbance capacity and cellular antioxidant activity assays indicated that digestive products of the EW-2 protein exhibited stronger antioxidant activity than those of the EW-1 protein. In summary, Se enrichment improved heat-induced gel properties of egg white, and promoted the gastrointestinal digestion of egg white protein.
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http://dx.doi.org/10.1016/j.foodchem.2021.130712DOI Listing
July 2021

PTCSC3-mediated glycolysis suppresses thyroid cancer progression via interfering with PGK1 degradation.

J Cell Mol Med 2021 Aug 1. Epub 2021 Aug 1.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.

The Warburg effect (aerobic glycolysis), a hallmark of cancer, serves as a promising target for diagnosis and therapy. Growing evidence indicates that long non-coding RNAs (lncRNAs) play an important role in aerobic glycolysis of various tumours. However, the correlation between lncRNAs and glycolysis in thyroid cancer cells is still poorly understood. In this study, we showed that lncRNA papillary thyroid cancer susceptibility candidate 3 (PTCSC3) was significantly downregulated in papillary thyroid carcinoma (PTC). Overexpression of PTCSC3 significantly inhibited the aerobic glycolysis and tumour growth of PTC cells. Consistently, PTCSC3 overexpression suppressed tumour progress in vivo. Mechanistically, PTCSC3 inhibits aerobic glycolysis and proliferation of PTC by directly interacting with PGK1, a key enzyme in glycolytic pathway. As a result, PTCSC3 performs its role in PTC development via PGK1 and may be a potential therapeutic target for PTC treatment.
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http://dx.doi.org/10.1111/jcmm.16806DOI Listing
August 2021

Genomic Analyses of Metaplastic or Sarcomatoid Carcinomas From Different Organs Revealed Frequent Mutations in KMT2D.

Front Mol Biosci 2021 15;8:688692. Epub 2021 Jul 15.

Department of Musculoskeletal Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Metaplastic or sarcomatoid carcinomas (MSCs) are rare epithelial malignancies with heterologous histological differentiation that can occur in different organs. The objective of the current study was to identify novel somatically mutated genes in MSCs from different organs. Whole-exome sequencing was performed in 16 paired MSCs originating from the breast ( = 10), esophagus ( = 3), lung ( = 2), and kidney ( = 1). In addition, we collected data on KMT2D mutations from eight independent cohorts ( = 195) diagnosed with MSCs derived from the breast ( = 83), liver ( = 8), esophagus ( = 15), lung ( = 10), and uterus or ovary ( = 79). The expression of KMT2D and its clinical significance were evaluated in our cohort. The most frequently mutated genes were TP53 (13/16, 81%) and KMT2D (5/16,31%). We identified seven somatic KMT2D mutations in the exploratory cohort ( = 16 tumors), including three nonsense mutations, two frameshift indels, one missense mutation, and one splice site mutation. Interestingly, two patients showed double hits on KMT2D with nonsense mutations and frameshift indels. In the eight validation cohorts ( = 195), the average mutation rates for TP53 and KMT2D were 78% (152/195) and 13% (25/195), respectively. Two or more hits on KMT2D were also present in three validation cohorts. Furthermore, KMT2D mutations were associated with low expression of KMT2D, large tumor size and unfavorable prognosis. These findings provide clues for understanding the genetic basis of MSCs originating from different organs and implicate KMT2D alteration as a frequent pathogenic mutation, allowing provision of appropriate treatment for this rare malignant disease in the future.
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http://dx.doi.org/10.3389/fmolb.2021.688692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319738PMC
July 2021

Risk factors for diagnosis of psoriatic arthritis, psoriasis, rheumatoid arthritis, and ankylosing spondylitis: A set of parallel case-control studies.

J Rheumatol 2021 Aug 1. Epub 2021 Aug 1.

Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States; Department of Medicine/Rheumatology, University of Iceland and Landspitali, Reykjavik, Iceland; Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States; Department of Medicine/Rheumatology, Boston University, Boston, Massachusetts, United States; Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States; Department of Medicine/Rheumatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States. Source of Support: This work was supported in part by NIH K23 AR063764 (PI Ogdie) and internal funds from the University of Pennsylvania. Maureen Dubreil was supported by NIH K23 AR06912701. Conflict of Interest: Elana Meer, Telma Thrastardottir, Maureen Dubreuil, Xingmei Wang, and Yong Chen report no conflicts of interest. Joel Gelfand served as a consultant for BMS, Boehringer Ingelheim, Lilly, Janssen Biologics, Novartis Corp, UCB (DSMB), Neuroderm (DSMB), Dr. Reddy's Labs, Pfizer Inc., and Sun Pharma, receiving honoraria; receives research grants (to the Trustees of the University of Pennsylvania) from Abbvie, Boehringer Ingelheim, Janssen, Novartis Corp, Celgene, Ortho Dermatologics, and Pfizer Inc.; and received payment for continuing medical education work related to psoriasis that was supported indirectly by Lilly, Ortho Dermatologics, and Novartis. Dr. Gelfand is a co-patent holder of resiquimod for treatment of cutaneous T cell lymphoma. Dr. Gelfand is a Deputy Editor for the Journal of Investigative Dermatology receiving honoraria from the Society for Investigative Dermatology and is a member of the Board of Directors for the International Psoriasis Council, receiving no honoraria. Thorvardur Love has received reimbursement from Celgene for speaking about guidelines for the treatment of psoriatic arthritis. Alexis Ogdie has served as a consultant for Abbvie, Amgen, BMS, Celgene, Corrona, Global Health Living Foundation, Janssen, Lilly, Novartis, Pfizer, and Takeda and has received grants to the University of Pennsylvania from Pfizer and Novartis and to Forward from Amgen. Her husband has received royalties from Novartis. Corresponding Author: Alexis Ogdie, MD MSCE, University of Pennsylvania, Division of Rheumatology, 3400 Civic Center Blvd., Philadelphia, Pennsylvania 19104;

Objective: To compare potential risk factors for the diagnosis of psoriatic arthritis (PsA), psoriasis, rheumatoid arthritis (RA), and ankylosing spondylitis (AS).

Methods: Four parallel case-control studies were conducted within The Health Improvement Network using data between 1994 and 2015. Patients with PsA, psoriasis, RA, or AS were identified using validated code lists and matched to controls on age, sex, practice, and year. Risk factors were selected in the time prior to diagnosis. Multivariable logistic regression models were constructed for each disease using automated stepwise regression to test potential risk factors.

Results: Patients with incident PsA (N=7,594), psoriasis (N=111,375), RA (N=28,341), and AS (N=3, 253) were identified and matched to 75,930, 1,113,345, 282,226, and 32,530 controls, respectively. Median diagnosis age was 48 (IQR 38-59), 41 (31-54), 43 (31-54), and 60 (48-71), respectively. In multivariable models, there were some shared and some differing risk factors across all 4 diseases: PsA was associated with obesity, pharyngitis, and skin infections; PsA and psoriasis were associated with obesity and moderate alcohol intake; PsA and AS were associated with uveitis; and PsA and RA were associated with preceding gout. Both RA and AS were associated with current smoking, former moderate drinking, anemia, osteoporosis, and inflammatory bowel disease. All shared former or current smoking as a risk factor; statin use was inversely associated with all 4 diseases.

Conclusion: Shared and different risk factors for PsA, psoriasis, RA, and AS were identified. Statin use was inversely associated with all 4 conditions.
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http://dx.doi.org/10.3899/jrheum.210006DOI Listing
August 2021

PIWI‑interacting RNA in cancer: Molecular mechanisms and possible clinical implications (Review).

Oncol Rep 2021 Sep 30;46(3). Epub 2021 Jul 30.

Department of Basic Medicine, Key Lab for Immunology in Universities of Shandong Province, Immunology Lab, Weifang Medical University, Weifang, Shandong 261053, P.R. China.

PIWI‑interacting RNA is a class of non‑coding small RNA that is ~30 nt long and is primarily found in mammalian germ cells from mice and humans. In cooperation with the members of PIWI protein family, this macromolecule participates in germ cell development, inhibits DNA self‑-replication and maintains genomic stability. Increasing evidence has demonstrated that PIWI‑interacting RNA (piRNAs) are abnormally expressed in various human cancers, such as liver cancer, stomach cancer, colorectal cancer, osteosarcoma, breast cancer, lung cancer, prostate cancer, etc. piRNAs abnormal expression is also associated with the occurrence and development of human cancers, such as liver cancer, stomach cancer, colorectal cancer, etc. Despite their unclear molecular mechanisms, piRNAs may act as oncogenes or tumor suppressors by interacting with multiple cancer‑related signal pathways including STAT3/Bcl‑xl or coding genes, such as heat shock transcription factor‑1. Hence, piRNAs may be potential markers and targets and provide new opportunities for cancer diagnosis, treatment or prognosis monitoring. The current review mainly aims to highlight the latest research progress made in the biological functions and regulation of piRNAs in mammals, their involvement in various cancer forms and their potential clinical applications.
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http://dx.doi.org/10.3892/or.2021.8160DOI Listing
September 2021

Esophagectomy versus definitive chemoradiotherapy as initial treatment for clinical stage I esophageal cancer: a systematic review and meta-analysis.

Dis Esophagus 2021 Jul 27. Epub 2021 Jul 27.

Department of Cardiothoracic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Esophagectomy and definitive chemoradiotherapy are commonly used in the treatment of stage I esophageal cancer (EC). The present study aims to compare the efficacy and safety of esophagectomy and definitive chemoradiotherapy as the initial treatment for clinical stage I EC.

Methods: This study was registered with the International Prospective Register of Systematic Reviews (CRD42020197203). Relevant studies were identified through PubMed, Web of Science, EMBASE, and Cochrane Library from database inception to June 30, 2020. Hazard ratio (HR) with 95% confidence intervals (CI) was employed to compare overall survival (OS) and progression-free survival (PFS). Odds ratio (OR) with 95% CI was employed to compare treatment-related death, complications, and tumor recurrence.

Results: A total of 13 non-randomized controlled studies involving 3,346 patients were included. Compared with definitive chemoradiotherapy, esophagectomy showed an improved OS (HR 0.69, 95% CI 0.55-0.86; P < 0.001), PFS (HR 0.47, 95% CI 0.33-0.67; P < 0.001), and a lower risk of tumor recurrence (OR 0.43, 95% CI 0.30-0.61; P < 0.001). There was no significant difference in the incidence of complications (OR 1.11, 95% CI 0.75-1.65; P = 0.60) and treatment-related death (OR 1.15, 95% CI 0.31-4.30; P = 0.84) between the two treatments.

Conclusions: Current evidence shows esophagectomy has superior survival benefits as the initial treatment for clinical stage I EC. It is still the preferred choice for patients with clinical stage I EC. However, future high-quality randomized controlled trials are needed to validate this conclusion.
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http://dx.doi.org/10.1093/dote/doab049DOI Listing
July 2021

Analysis of Cholangitis Rates with Extended Perioperative Antibiotics and Adjuvant Corticosteroids in Biliary Atresia.

Pediatr Gastroenterol Hepatol Nutr 2021 Jul 5;24(4):366-376. Epub 2021 Jul 5.

Gastroenterology, Hepatology & Nutrition Service, Pediatric Medicine, KK Women's and Children's Hospital, Singapore.

Purpose: There is no consensus regarding adjuvant therapies following Kasai portoenterostomy (KP) for biliary atresia (BA). This study aimed to analyze the effect of extended perioperative intravenous antibiotics (PI-Abx) and adjuvant corticosteroid on cholangitis and jaundice clearance rates in the 3 years post-KP in children with BA.

Methods: Data of patients who underwent KP between 1999-2018 at a single center were retrospectively analyzed. Group A (1999-2010) received PI-Abx for 5 days, Group B (2010-2012) received PI-Abx for 5 days plus low-dose prednisolone (2 mg/kg), and Group C (2012-2017) received PI-Abx for 14 days plus high-dose prednisolone (5 mg/kg).

Results: Fifty-four patients were included with groups A, B, and C comprising 25, 9, and 20 patients, respectively. The number of episodes of cholangitis was 1.0, 1.6, and 1.3 per patient (=NS) within the first year and 1.8, 2.3, and 1.7 (=NS) over 3 years in Groups A, B, and C, respectively. The jaundice clearance rate at 6 months was 52%, 78%, and 50% (=NS), and the 3-year native liver survival (NLS) rate was 76%, 100%, and 80% (=NS) in Groups A, B, and C, respectively. A near-significant association was observed between the incidence of cholangitis within the first year and decompensated liver cirrhosis/death at 3 years post KP (=0.09). Persistence of jaundice at 6 months was significantly associated with decompensated cirrhosis/death at 3 years (<0.001).

Conclusion: The extended duration of PI-Abx and adjuvant corticosteroids was not associated with improved rates of cholangitis, jaundice clearance, or NLS in patients with BA.
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http://dx.doi.org/10.5223/pghn.2021.24.4.366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279824PMC
July 2021

Evaluation of dual-energy CT derived radiomics signatures in predicting outcomes in patients with advanced gastric cancer after neoadjuvant chemotherapy.

Eur J Surg Oncol 2021 Jul 20. Epub 2021 Jul 20.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No 197, Rui Jin 2nd Road, Shanghai, 200025, China. Electronic address:

Background: To investigate the prognostic value of dual-energy CT (DECT) based radiomics to predict disease-free survival (DFS) and overall survival (OS) for patients with advanced gastric cancer (AGC) after neoadjuvant chemotherapy (NAC).

Methods: From January 2014 to December 2018, a total of 156 AGC patients were enrolled and randomly allocated into a training cohort and a testing cohort at a ratio of 2:1. Volume of interest of primary tumor was delineated on eight image series. Four feature sets derived from pre-NAC and delta radiomics were generated for each survival arm. Random survival forest was used for generating the optimal radiomics signature (RS). Statistical metrics for model evaluation included Harrell's concordance index (C-index) and the average cumulative/dynamic AUC throughout follow-up. A clinical model and a combined Rad-clinical model were built for comparison.

Results: The pre-IU (derived from iodine uptake images before NAC) RS performed best for DFS and OS in the testing cohort (C-indices, 0.784 and 0.698; the average cumulative/dynamic AUCs, 0.80 and 0.77). When compared with the clinical model, the radiomics model had significantly higher C-index to predict DFS in the testing cohort (0.784 vs. 0.635, p < 0.001), but no statistical difference was found for OS (0.698 vs. 0.680, p = 0.473). The combined Rad-clinical models showed improved performance in the testing cohort, with C-indices of 0.810 and 0.710 for DFS and OS, respectively.

Conclusion: DECT-derived radiomics serves as a promising non-invasive biomarker to predict survival for AGC patients after NAC, providing an opportunity for transforming proper treatment.
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http://dx.doi.org/10.1016/j.ejso.2021.07.014DOI Listing
July 2021

[Clinical application of SVF-gel in unilateral vocal cord paralysis].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 May;35(5):395-399

Department of Otorhinolaryngology Head and Neck Surgery,Nanjing Tongren Hospital,School of Medicine,Southeast University,Nanjing Tongren ENT Hospital,Nanjing,210000,China.

To investigate the therapeutic effect of SVF-gel in unilateral vocal cord paralysis. his investigation presented a retrospective study of SVF-gel auto-transplantation in 22 patients with unilateral vocal cord paralysis from June 2017 to December 2018 in otolaryngology head and neck surgery of Nanjing Tongren Hospital. Under the general anesthesia support laryngoscope, the medial thigh was selected as the fat supply area. SVF-gel was obtained by SVF-gel technology. The SVF-gel was transplanted into the vocal cord muscle and the paraglottic space. The laryngography was performed before and after the operation. The voice was analyzed and compared to evaluate the therapeutic effect. SVF-gel was successfully transplanted in all 22 cases. The stroboscopic laryngoscopy showed that the vocal cords vibrated well and the glottis closed normally. The results of GRBAS showed that the voice quality of the patients at 12 and 18 months after the operation was significantly improved. The voice analysis showed that the maximum phonation time of the patients at 12 and 18 months after the operation increased. The basic frequency increased. The basic frequency perturbation, amplitude perturbation and standardized noise energy decreased significantly(<0.05). SVF-gel auto-transplantation can effectively improve the voice quality of unilateral vocal cord paralysis and the long-term effect is stable.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.05.003DOI Listing
May 2021

Emerging Role of Transient Receptor Potential Vanilloid 4 (TRPV4) Ion Channel in Acute and Chronic Itch.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

Department of Neurology, Duke University, Durham, NC 27710, USA.

Itch is a clinical problem that leaves many sufferers insufficiently treated, with over 20 million cases in the United States. This is due to incomplete understanding of its molecular, cellular, and cell-to-cell signaling mechanisms. Transient receptor potential (TRP) ion channels are involved in several sensory modalities including pain, vision, taste, olfaction, hearing, touch, and thermosensation, as well as itch. Relative to the extensive studies on TRPV1 and TRPA1 ion channels in itch modulation, TRPV4 has received relatively little research attention and its mechanisms have remained poorly understood until recently. TRPV4 is expressed in ganglion sensory neurons and a variety of skin cells. Growing evidence in the past few years strongly suggests that TRPV4 in these cells contributes to acute and chronic disease-associated itch. This review focuses on the current experimental evidence involving TRPV4 in itch under pathophysiological conditions and discusses its possible cellular and molecular mechanisms.
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http://dx.doi.org/10.3390/ijms22147591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307539PMC
July 2021

[A 1-hour Bundle compliance survey of the "Surviving Sepsis Campaign" and its impact on the prognosis of sepsis patients: a multicenter, prospective observational cohort study].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Jun;33(6):671-675

Department of Critical Care, Yanjiang District People's Hospital, Ziyang 641300, Sichuan, China. Corresponding author: Liao Lankai, Email:

Objective: To investigate clinicians' compliance with the 2018 Surviving Sepsis Campaign (SSC) update "1-hour sepsis Bundle therapy" (1-hour Bundle) when treating patients with Sepsis 3 in the intensive care unit (ICU), and to analyze its impact on patient outcomes.

Methods: A multicenter, prospective observational cohort study was conducted. A total of 153 ICU patients in Ziyang First People's Hospital, Ziyang People's Hospital and Yanjiang District People's Hospital who were diagnosed of sepsis by the definition and diagnostic criteria of Sepsis 3 from January 2019 to December 2020 were selected. Among them, 95 patients who had completed 1-hour Bundle were divided into the Bundle compliance group. 58 patients who did not complete the Bundle within 1 hours were classified as the Bundle non-compliance group. The distribution of pathogenic bacteria and infected sites, 1-hour Bundle compliance and 28-day survival in the 3 hospitals were analyzed. Univariate analysis was used to analyze the risk factors affecting the prognostic between the two groups of sepsis patients. Cox regression model was used to draw a 28-day survival curve to evaluate the survival of the patients in the two groups.

Results: Among 153 sepsis patients in 3 hospitals, the detection rate of pathogenic bacteria was 61.44% (94/153), and Gram-negative bacteria accounted for 79.79% (75/94). The top 3 infection sites were respiratory system, gastrointestinal tract and urinary system, accounted for 32.0%, 28.1% and 18.3%, respectively. In the 3 hospitals, 62.09% (95/153) of patients fully implemented the 1-hour Bundle. The poorly implemented indicators in the 1-hour Bundle were 1-hour blood microbial culture [77.78% (119/153)] and 1-hour antimicrobial application [79.74% (122/153)]. There was no significant difference in the baseline indicators between Bundle compliance and non-compliance groups. Univariate analysis showed that the main prognostic indicators: 28-day survival rate in the Bundle compliance group was significantly higher than that in the Bundle non-compliance group [80.00% (76/95) vs. 62.06% (36/58), χ = 6.447, P = 0.014]. Secondary evaluation indicators: mean arterial pressure (MAP) at 6 hours and 24 hours in the Bundle compliance group were significantly higher than those in the Bundle non-compliance group [mmHg (1 mmHg = 0.133 kPa): 78.22±11.25 vs. 69.86±14.04, 79.78±11.45 vs. 75.35±12.90]. However, the median length of in hospital stay in the Bundle compliance group was significantly longer than that in the Bundle non-compliance group [days: 13 (17) vs. 6 (11)], with statistically significant differences (all P < 0.05). Bivariate Logistic regression analysis showed that 6 hours and 24 hours MAP were risk factors affecting the prognosis of patients with sepsis [odds ratio (OR), 95% confidence interval (95%CI): 1.064 (0.994-1.102), 1.032 (1.003-1.063), both P < 0.05].

Conclusions: The 1-hour Bundle compliance rate of ICU patients with sepsis in 3 hospitals of Ziyang City was 62.09%, and the compliance is still to be improved, especially for the 2 aspects of empirical antimicrobial use and microbial culture retention before antimicrobial use. The 28-day survival rate in the Bundle compliance group was significantly higher than that in the Bundle non-compliance group, suggesting that the 1-hour Bundle regimen can improve the prognosis of patients with sepsis.
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http://dx.doi.org/10.3760/cma.j.cn121430-20210408-00520DOI Listing
June 2021

IRE1α regulates skeletal muscle regeneration through Myostatin mRNA decay.

J Clin Invest 2021 Jul 20. Epub 2021 Jul 20.

College of Life Sciences, Wuhan University, Wuhan, China.

Skeletal muscle can undergo a regenerative process from injury or disease to preserve muscle mass and function, which is critically influenced by cellular stress responses. Inositol-requiring enzyme 1 (IRE1) is an ancient endoplasmic reticulum (ER) stress sensor and mediates a key branch of the unfolded protein response (UPR). In mammals, IRE1α is implicated in the homeostatic control of stress responses during tissue injury and regeneration. Here, we show that IRE1α serves as a myogenic regulator in skeletal muscle regeneration in response to injury and muscular dystrophy. We found in mice that IRE1α was activated during injury-induced muscle regeneration, and muscle-specific IRE1α ablation resulted in impaired regeneration upon cardiotoxin-induced injury. Gain- and loss-of-function studies in myocytes demonstrated that IRE1αacts to sustain both differentiation in myoblasts and hypertrophy in myotubes through regulated IRE1-dependent decay (RIDD) of mRNA encoding Myostatin, a key negative regulator of muscle repair and growth. Furthermore, in the mouse model of Duchenne muscular dystrophy (DMD), loss of muscle IRE1α resulted in augmented Myostatin signaling and exacerbated the dystrophic phenotypes. Thus, these results reveal a pivotal role for the RIDD output of IRE1α in muscle regeneration, offering new insight into potential therapeutic strategies for muscle loss diseases.
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http://dx.doi.org/10.1172/JCI143737DOI Listing
July 2021

Why Is the Electronic Health Record So Challenging for Research and Clinical Care?

Methods Inf Med 2021 Jul 19. Epub 2021 Jul 19.

Department of Biostatistics, Epidemiology, and Informatics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, United States.

Background:  The electronic health record (EHR) has become increasingly ubiquitous. At the same time, health professionals have been turning to this resource for access to data that is needed for the delivery of health care and for clinical research. There is little doubt that the EHR has made both of these functions easier than earlier days when we relied on paper-based clinical records. Coupled with modern database and data warehouse systems, high-speed networks, and the ability to share clinical data with others are large number of challenges that arguably limit the optimal use of the EHR OBJECTIVES:  Our goal was to provide an exhaustive reference for those who use the EHR in clinical and research contexts, but also for health information systems professionals as they design, implement, and maintain EHR systems.

Methods:  This study includes a panel of 24 biomedical informatics researchers, information technology professionals, and clinicians, all of whom have extensive experience in design, implementation, and maintenance of EHR systems, or in using the EHR as clinicians or researchers. All members of the panel are affiliated with Penn Medicine at the University of Pennsylvania and have experience with a variety of different EHR platforms and systems and how they have evolved over time.

Results:  Each of the authors has shared their knowledge and experience in using the EHR in a suite of 20 short essays, each representing a specific challenge and classified according to a functional hierarchy of interlocking facets such as usability and usefulness, data quality, standards, governance, data integration, clinical care, and clinical research.

Conclusion:  We provide here a set of perspectives on the challenges posed by the EHR to clinical and research users.
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http://dx.doi.org/10.1055/s-0041-1731784DOI Listing
July 2021

Enhancing the graphene photocurrent using surface plasmons and a p-n junction.

Light Sci Appl 2020 Jul 20;9(1):126. Epub 2020 Jul 20.

School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.

The recently proposed concept of graphene photodetectors offers remarkable properties such as unprecedented compactness, ultrabroadband detection, and an ultrafast response speed. However, owing to the low optical absorption of pristine monolayer graphene, the intrinsically low responsivity of graphene photodetectors significantly hinders the development of practical devices. To address this issue, numerous efforts have thus far been made to enhance the light-graphene interaction using plasmonic structures. These approaches, however, can be significantly advanced by leveraging the other critical aspect of graphene photoresponsivity enhancement-electrical junction control. It has been reported that the dominant photocarrier generation mechanism in graphene is the photothermoelectric (PTE) effect. Thus, the two energy conversion mechanisms involved in the graphene photodetection process are light-to-heat and heat-to-electricity conversions. In this work, we propose a meticulously designed device architecture to simultaneously enhance the two conversion efficiencies. Specifically, a gap plasmon structure is used to absorb a major portion of the incident light to induce localized heating, and a pair of split gates is used to produce a p-n junction in graphene to augment the PTE current generation. The gap plasmon structure and the split gates are designed to share common key components so that the proposed device architecture concurrently realizes both optical and electrical enhancements. We experimentally demonstrate the dominance of the PTE effect in graphene photocurrent generation and observe a 25-fold increase in the generated photocurrent compared to the un-enhanced cases. While further photocurrent enhancement can be achieved by applying a DC bias, the proposed device concept shows vast potential for practical applications.
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http://dx.doi.org/10.1038/s41377-020-00344-1DOI Listing
July 2020

An unusual case of symptomatic portal hypertension.

Gastroenterology 2021 Jul 16. Epub 2021 Jul 16.

Division of Vascular and Interventional Radiology, Department of General Surgery, Nanfang Hospital, Southern Medical University. Electronic address:

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http://dx.doi.org/10.1053/j.gastro.2021.07.019DOI Listing
July 2021

Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects.

Biotechnol Adv 2021 Jul 8:107794. Epub 2021 Jul 8.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address:

The oleaginous fungus Mortierella alpina has distinct advantages in long-chain PUFAs production, and it is the only source for dietary arachidonic acid (ARA) certificated by FDA and European Commission. This review provides an overall introduction to M. alpina, including its major research methods, key factors governing lipid biosynthesis, metabolic engineering and omics studies. Currently, the research interests in M. alpina focus on improving lipid yield and fatty acid desaturation degree by enhancing fatty acid precursors and the reducing power NADPH, and genetic manipulation on PUFAs synthetic pathways is carried to optimise fatty acid composition. Besides, multi-omics studies have been applied to elucidate the global regulatory mechanism of lipogenesis in M. alpina. However, research challenges towards achieving a lipid cell factory lie in strain breeding and cost control due to the coenocytic mycelium, long fermentation period and insufficient conversion rate from carbon to lipid. We also proposed future research goals based on a multilevel regulating strategy: obtaining ideal chassis by directional evolution and high-throughput screening; rewiring central carbon metabolism and inhibiting competitive pathways by multi-gene manipulation system to enhance carbon to lipid conversion rate; optimisation of protein function based on post-translational modification; application of dynamic fermentation strategies suitable for different fermentation phases. By reviewing the comprehensive research progress of this oleaginous fungus, we aim to further comprehend the fungal lipid metabolism and provide reference information and guidelines for the exploration of microbial oils from the perspectives of fundamental research to industrial application.
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http://dx.doi.org/10.1016/j.biotechadv.2021.107794DOI Listing
July 2021

Bayesian meta-analysis using SAS PROC BGLIMM.

Res Synth Methods 2021 Jul 10. Epub 2021 Jul 10.

Division of Biostatistics, University of Minnesota School of Public Health, Minneapolis, Minnesota, USA.

Meta-analysis is commonly used to compare two treatments. Network meta-analysis (NMA) is a powerful extension for comparing and contrasting multiple treatments simultaneously in a systematic review of multiple clinical trials. Although the practical utility of meta-analysis is apparent, it is not always straightforward to implement, especially for those interested in a Bayesian approach. This paper demonstrates that the recently-developed SAS procedure BGLIMM provides an intuitive and computationally efficient means for conducting Bayesian meta-analysis in SAS, using a worked example of a smoking cessation NMA data set. BGLIMM gives practitioners an effective and simple way to implement Bayesian meta-analysis (pairwise and network, either contrast-based or arm-based) without requiring significant background in coding or statistical modeling. Those familiar with generalized linear mixed models, and especially the SAS procedure GLIMMIX, will find this tutorial a useful introduction to Bayesian meta-analysis in SAS.
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http://dx.doi.org/10.1002/jrsm.1513DOI Listing
July 2021

Phase management in single-crystalline vanadium dioxide beams.

Nat Commun 2021 Jul 9;12(1):4214. Epub 2021 Jul 9.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, People's Republic of China.

A systematic study of various metal-insulator transition (MIT) associated phases of VO, including metallic R phase and insulating phases (T, M1, M2), is required to uncover the physics of MIT and trigger their promising applications. Here, through an oxide inhibitor-assisted stoichiometry engineering, we show that all the insulating phases can be selectively stabilized in single-crystalline VO beams at room temperature. The stoichiometry engineering strategy also provides precise spatial control of the phase configurations in as-grown VO beams at the submicron-scale, introducing a fresh concept of phase transition route devices. For instance, the combination of different phase transition routes at the two sides of VO beams gives birth to a family of single-crystalline VO actuators with highly improved performance and functional diversity. This work provides a substantial understanding of the stoichiometry-temperature phase diagram and a stoichiometry engineering strategy for the effective phase management of VO.
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http://dx.doi.org/10.1038/s41467-021-24527-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270972PMC
July 2021

Efficient synthesis of vinylene-linked conjugated porous networks the Horner-Wadsworth-Emmons reaction for photocatalytic hydrogen evolution.

Chem Commun (Camb) 2021 Jul;57(61):7557-7560

State Key Laboratory for Oxo Synthesis and Selective Oxidation, Suzhou Research Institute of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Suzhou 215000, China.

A simple, yet efficient synthetic approach for the construction of vinylene-linked conjugated porous networks was developed. Based on the Horner-Wadsworth-Emmons reaction, the condensation polymerization for the formation of an sp2 carbon-linkage can be achieved at room temperature. The resulting vinylene-linked frameworks exhibit a promising porous nature with the best surface area of up to 1373 m2 g-1. Their intrinsic conjugated architectures and semiconducting properties lead to photocatalytic evolution of hydrogen from water under visible light irradiation. This new synthesis method provides a facile means to prepare attractive sp2 carbon linked porous organic frameworks.
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http://dx.doi.org/10.1039/d1cc02280fDOI Listing
July 2021

Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.

Langmuir 2021 07 8;37(28):8486-8497. Epub 2021 Jul 8.

Key Laboratory of Special Energy Materials Ministry of Education, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China.

Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties. Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology. The core-shell structure and the composition of energetic [email protected] were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses. The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly. In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process. In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding. Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
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http://dx.doi.org/10.1021/acs.langmuir.1c00874DOI Listing
July 2021

A case report of atypical hemiplegic migraine with nonheadache onset in a Chinese child.

BMC Neurol 2021 Jul 6;21(1):267. Epub 2021 Jul 6.

Department of Neurology, Children's Hospital of Jiangxi Province, Nanchang, 330006, China.

Background: Hemiplegic migraine (HM) is an uncommon subtype of migraine with aura including motor weakness. The core symptoms of HM are headache and motor weakness. However, we report a rare case of atypical HM with nonheadache onset in a Chinese child who was misdiagnosed several times.

Case Presentation: We report a Chinese boy whose onset was sudden when he was 3 years old. He presented with a variety of phenotypes, including fever, vomiting, alternating hemiplegia, and drowsiness, but no headache in the initial stages. Magnetic resonance imaging (MRI) demonstrated unilateral cerebral oedema during the initial episode of hemiplegia. These symptoms recurred many times. As the disease progressed, the patient developed episodic headache. The patient was misdiagnosed several times with encephalitis, alternating hemiplegia of childhood (AHC) and mitochondrial encephalopathy. Whole-exome next-generation sequencing revealed a de novo heterozygous missense mutation in the ATP1A2 gene(p.Gly715Arg) classified as pathogenic and eventually led to a diagnosis of HM when he was 11 years old. Flunarizine was subsequently administered, and no recurrence was found during follow-up.

Conclusions: HM in children may be atypical in the initial stage of the disease, which could manifest as fever, alternating hemiplegia and drowsiness but no headache at the onset. This could easily lead to misdiagnosis. With age, it may eventually manifest as typical HM. Therefore, attention should be given to differentiation in clinical practice.When similar clinical cases appear, gene detection is particularly important, which is conducive to early diagnosis and treatment.
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http://dx.doi.org/10.1186/s12883-021-02302-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258926PMC
July 2021

Severe hematuria due to vesical varices in a patient with portal hypertension: A case report.

World J Clin Cases 2021 Jun;9(18):4810-4816

Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Background: Hematuria is one of the most common clinical symptoms for urologists and is typically observed in urinary system tumors, prostate hyperplasia, and urinary stone disease. Hematuria due to vesical varices is very rare, and only a few cases have been reported since 1989. We report the first case of vesical varices due to portal hypertension with aberrant development and functioning of the genitourinary system along with the complete diagnosis and treatment process.

Case Summary: This patient was a 53-year-old man with a history of aberrant development of the genitourinary system and hepatitis B-associated cirrhosis. He was admitted to the emergency department with severe hematuria and bladder clot tamponade. Many abnormally dilated blood vessels were found surrounding the bladder in the pelvis by color Doppler ultrasound, contrast-enhanced computed tomography, and three-dimensional visualization technology. It was difficult to perform transurethral cystoscopy and hemostasis in this patient, so we performed open surgical bladder exploration for hemostasis and surgical devascularization around the bladder.

Conclusion: Urologists should improve the understanding of the pathophysiology, clinical manifestations, diagnosis, and treatment of vesical varices. This case may be presented as a reference for the diagnosis and management of severe hematuria due to vesical varices.
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http://dx.doi.org/10.12998/wjcc.v9.i18.4810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223820PMC
June 2021

Electrochemical Oscillation during Galvanostatic Charging of LiCrTiO in Li-Ion Batteries.

Materials (Basel) 2021 Jun 29;14(13). Epub 2021 Jun 29.

State Key Laboratory on Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory of Research on Utilization of Si-Zr-Ti Resources, School of Materials Science and Engineering, Hainan University, Haikou 570228, China.

In the late 1960s, the establishment of Prigogine's dissipative structure theory laid the foundation for the (electro)chemical oscillation phenomenon, which has been widely investigated in some electrochemical reactions, such as electro-catalysis and electro-deposition, while the electrochemical oscillation of Li-ion batteries has just been discovered in spinel LiTiO a few years before. In this work, spinel LiCrTiO samples were synthesized by using a high-temperature solid-state method, characterized with SEM (Scanning electron microscope), XRD (X-ray diffraction), Raman and XPS (X-ray photoelectron spectroscopy) measurements, and electrochemically tested in Li-ion batteries to study the electrochemical oscillation. When sintering in a powder form at a temperature between 800 and 900 °C, we achieved the electrochemical oscillation of spinel LiCrTiO during charging, and it is suppressed in the non-stoichiometric LiCrTiO samples, especially for reducing the Li content or increasing the Cr content. Therefore, this work developed another two-phase material as the powder-sintered LiCrTiO exhibiting the electrochemical oscillation in Li-ion batteries, which would inspire us to explore more two-phase electrode materials in Li-ion batteries, Na-ion batteries, etc.
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http://dx.doi.org/10.3390/ma14133624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269718PMC
June 2021

Elasto-Plastic Fracture Mechanics Analysis of the Effect of Shot Peening on 300M Steel.

Materials (Basel) 2021 Jun 25;14(13). Epub 2021 Jun 25.

National Engineering Research Center for Remanufacturing, Army Academy of Armored Forces, Beijing 100072, China.

A modified J-integral calculation method is adopted to fix the problem of the quantitative evaluation of the crack propagation of shot-peened structures. Considering the residual stress, residual strain, and residual strain energy, the effect of shot peening on the J-integral parameters of semi-elliptic surface crack fronts is quantitatively calculated and a method is provided for the performance evaluation of the shot peening layer. First, the shot peening process is simulated, then the fatigue crack is generated by changing the constraint condition and a far-field load is applied to calculate the J-integral parameters, crack propagation rate, and crack kinking angle. The effects of different crack depths and shot velocities on the fracture parameters are analyzed. The results show that the reduction in the J-integral value after shot peening decreases with the increase in the crack depth when the shot velocity is a certain value, which indicates that shot peening is more beneficial for suppressing the fatigue crack propagation. When the crack depth is greater than the depth of the compressive stress layer, shot peening accelerates the crack propagation. The reduction in the J-integral value decreases with the increase in shot velocity when the crack depth is a certain value; therefore, increasing shot velocity is more beneficial for retarding fatigue crack propagation.
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http://dx.doi.org/10.3390/ma14133538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269553PMC
June 2021

Preparation of LiFePO Powders by Ultrasonic Spray Drying Method and Their Memory Effect.

Materials (Basel) 2021 Jun 10;14(12). Epub 2021 Jun 10.

State Key Laboratory on Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory of Research on Utilization of Si-Zr-Ti Resources, School of Materials Science and Engineering, Hainan University, Haikou 570228, China.

The memory effect of lithium-ion batteries (LIBs) was first discovered in LiFePO, but its origin and dependence are still not clear, which is essential for regulating the memory effect. In this paper, a home-made spray drying device was used to successfully synthesize LiFePO with an average particle size of about 1 μm, and we studied the influence of spray drying temperature on the memory effect of LiFePO in LIBs. The results showed that the increasing of spray drying temperature made the memory effect of LiFePO strengthen from 1.3 mV to 2.9 mV, while the capacity decreased by approximately 6%. The XRD refinement and FTIR spectra indicate that the enhancement of memory effect can be attributed to the increment of Li-Fe dislocations. This work reveals the dependence of memory effect of LiFePO on spray drying temperature, which will guide us to optimize the preparation process of electrode materials and improve the management system of LIBs.
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http://dx.doi.org/10.3390/ma14123193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230317PMC
June 2021

Long non-coding RNA SNHG16 regulates E2F1 expression by sponging miR-20a-5p and aggravating proliferative diabetic retinopathy.

Can J Physiol Pharmacol 2021 Jul 1. Epub 2021 Jul 1.

Zhuji Affiliated Hospital of Wenzhou Medical University, 74784, 9, Jianmin Road, Taozhu Street, Zhuji City, Zhuji, China, 311800;

Long non-coding RNAs (lncRNAs) were reported that related to microvascular dysfunction in diabetic retinopathy (DR), but the potential mechanism remains unknown. This study was designed to elucidate the effects of lncRNA SNHG16 in proliferative DR progression. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the levels of SNHG16 and miR-20a-5p from peripheral blood samples of different participants. Pearson's correlation analysis on the plasma data was applied to detect correlations between SNHG16 and miR-20a-5p. Finally, the interactions of miR-20a-5p and SNHG16 or E2F1 were assessed by luciferase reporter assays. SNHG16 and E2F1 were increased and miR-20a-5p was decreased in proliferative DR both in vivo and in vitro, when compared with control or non-proliferative DR. E2F1 was identified as the target of miR-20a-5p. MiR-20a-5p interacted with SNHG16 and E2F1, and was controlled by SNHG16. The regulation of SNHG16 on E2F1 was mediated by miR-20a-5p. Cells transfected with SNHG16 OE plasmid markedly increased cell apoptosis and vessel-like formation, whereas the miR-20a-5p mimic partially reversed these effects. Transfection with si-E2F1 plasmid rescued SNHG16 overexpression-aggravated proliferative DR. This study indicated that SNHG16 regulated E2F1 expression by sponging miR-20a-5p and aggravating proliferative DR.
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http://dx.doi.org/10.1139/cjpp-2020-0693DOI Listing
July 2021

Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize.

BMC Plant Biol 2021 Jun 30;21(1):305. Epub 2021 Jun 30.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.

Background: Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection.

Results: In the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5'-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5'-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance.

Conclusions: ZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene.
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http://dx.doi.org/10.1186/s12870-021-03072-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243440PMC
June 2021

Ligustilide enhances hippocampal neural stem cells activation to restore cognitive function in the context of postoperative cognitive dysfunction.

Eur J Neurosci 2021 Jun 30. Epub 2021 Jun 30.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Ligustilide exerts potential neuroprotective effects against various cerebral ischaemic insults and neurodegenerative disorders. However, the function and mechanisms of LIG-mediated hippocampal neural stem cells (H-NSCs) activation as well as cognitive recovery in the context of post-operative cognitive dysfunction (POCD) remain elusive and need to be explored. Mice were subjected to transient global cerebral ischaemia and reperfusion (tGCI/R) injury and treated with LIG (80 mg/kg) or vehicle for 1 month. Morris water maze test and western blot were employed to assess cognitive function. Nissl staining and immunofluorescence (IF) staining were used to detect H-NSCs proliferation and neurogenesis in hippocampus. Subsequently, primary H-NSCs were treated with LIG, and the level of H-NSCs proliferation and neuronal-differentiation was examined by IF staining for Edu and β-Tubulin III. The protein levels of ERK1/2, β-catenin, NICD, TLR4, Akt and FoxO1 were examined using western blotting. Finally, pretreatment with the ERK agonist SCH772984 was performed to observe the change in ERK expression. LIG treatment promoted H-NSCs proliferation and neurogenesis, increased the number of neurons in the hippocampal subfields, and ultimately reversed cognitive impairment in tGCI/R injury. Furthermore, LIG also promoted primary H-NSCs proliferation and neuronal-differentiation, as well as ERK1/2 phosphorylation. Pretreatment with SCH772984 effectively reversed the ability of LIG to induce ERK1/2 phosphorylation and promote H-NSCs proliferation and neuronal-differentiation. LIG can promote cognitive recovery after tGCI/R injury by activating ERK1/2 in H-NSCs to promote their proliferation and neurogenesis in the hippocampus. Therefore, LIG has potential for use in the prevention and/or treatment of POCD.
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http://dx.doi.org/10.1111/ejn.15363DOI Listing
June 2021
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