Publications by authors named "Yixin Yang"

94 Publications

Nanoscale Covalent Organic Frameworks with Donor-Acceptor Structures as Highly Efficient Light-Responsive Oxidase-like Mimics for Colorimetric Detection of Glutathione.

ACS Appl Mater Interfaces 2021 Oct 12;13(41):49482-49489. Epub 2021 Oct 12.

Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.

Although organic artificial enzymes have been reported as biomimetic oxidation catalysts and are widely used for colorimetric biosensors, developing organic artificial enzymes with high enzymatic activity is still a challenge. Two-dimensional (2D) covalent organic frameworks (COFs) have shown superior potential in biocatalysts because of their periodic π-π arrays, tunable pore size and structure, large surface area, and thermal stability. The interconnection of electron acceptor and donor building blocks in the 2D conjugated COF skeleton can lead to narrower band gaps and efficient charge separation and transportation and thus is helpful to improve catalytic activity. Herein, a donor-acceptor 2D COF was synthesized using tetrakis(4-aminophenyl)pyrene (Py) as an electron donor and thieno[3,2-]thiophene-2,5-dicarbaldehyde (TT) as an electron acceptor. Under visible light irradiation, the donor-acceptor 2D COF exhibited superior enzymatic catalytic activity, which could catalyze the oxidation of chromogenic substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) by the formation of superoxide radicals and holes. Based on the above property, the photoactivated donor-acceptor 2D COF with enzyme-like catalytic properties was designed as a robust colorimetric probe for cheap, highly sensitive, and rapid colorimetric detection of glutathione (GSH); the corresponding linear range of GSH was 0.4-60 μM, and the limit of detection was 0.225 μM. This study not only presents the construction of COF-based light-activated nanozymes for environmentally friendly colorimetric detection of GSH but also provides a smart strategy for improving nanozyme activity.
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http://dx.doi.org/10.1021/acsami.1c13997DOI Listing
October 2021

Facile synthesis of Ti-immobilized magnetic covalent organic frameworks for enhanced phosphopeptide enrichment.

Talanta 2021 Dec 6;235:122789. Epub 2021 Aug 6.

Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China. Electronic address:

In this work, core-shell structured Ti-immobilized magnetic covalent organic frameworks (denoted as [email protected] composites) were prepared for enhanced phosphopeptide enrichment by one-pot synthesis of COFs shell with inherent bifunctional groups on FeO NPs and further Ti immobilization. The widely distributed bifunctional groups could provide abundant chelating sites for Ti immobilizing. Combining with the high specific surface area and mesoporous structure, the [email protected] composites exhibited excellent enrichment efficiency for phosphopeptides, such as low detection limit (0.05 fmol μL), high selectivity (1:5000 of molar ratio of β-casein/bovine serum albumin (BSA) tryptic digests), high adsorption capacity (62.9 μg mg) and strong size-exclusive effect (1:250:250 of molar ratio of β-casein tryptic digest/β-casein/BSA). In addition, this method was general for immobilizing other metal ions (Zr and Fe). Notably, the [email protected] composites exhibited controllable affinity towards mono-phosphopeptides and multi-phosphopeptides. Furthermore, the [email protected] composites were successfully applied to selectively capture phosphopeptides from complex biological samples including the tryptic digest of nonfat milk, human serum and human saliva. More significantly, 3333 phosphopeptides derived from 1409 phosphoproteins with 3492 phosphorylation sites were clearly identified from the tryptic digest of HeLa cell lysate. In addition to providing a potential excellent enrichment probe for comprehensive phosphoproteomic analysis, this study also offers a new perspective for the functionalization of COFs.
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http://dx.doi.org/10.1016/j.talanta.2021.122789DOI Listing
December 2021

Adaptive Two-Step Bearing-Only Underwater Uncooperative Target Tracking with Uncertain Underwater Disturbances.

Entropy (Basel) 2021 Jul 16;23(7). Epub 2021 Jul 16.

College of Underwater Acoustic Engineering, Harbin Engineering University, Harbin 150001, China.

The bearing-only tracking of an underwater uncooperative target can protect maritime territories and allows for the utilization of sea resources. Considering the influences of an unknown underwater environment, this work aimed to estimate 2-D locations and velocities of an underwater target with uncertain underwater disturbances. In this paper, an adaptive two-step bearing-only underwater uncooperative target tracking filter (ATSF) for uncertain underwater disturbances is proposed. Considering the nonlinearities of the target's kinematics and the bearing-only measurements, in addition to the uncertain noise caused by an unknown underwater environment, the proposed ATSF consists of two major components, namely, an online noise estimator and a robust extended two-step filter. First, using a modified Sage-Husa online noise estimator, the uncertain process and measurement noise are estimated at each tracking step. Then, by adopting an extended state and by using a robust negative matrix-correcting method in conjunction with a regularized Newton-Gauss iteration scheme, the current state of the underwater uncooperative target is estimated. Finally, the proposed ATSF was tested via simulations of a 2-D underwater uncooperative target tracking scenario. The Monte Carlo simulation results demonstrated the reliability and accuracy of the proposed ATSF in bearing-only underwater uncooperative tracking missions.
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http://dx.doi.org/10.3390/e23070907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305256PMC
July 2021

3D Underwater Uncooperative Target Tracking for a Time-Varying Non-Gaussian Environment by Distributed Passive Underwater Buoys.

Entropy (Basel) 2021 Jul 15;23(7). Epub 2021 Jul 15.

College of Underwater Acoustic Engineering, Harbin Engineering University, Harbin 150001, China.

Accurate 3D passive tracking of an underwater uncooperative target is of great significance to make use of the sea resources as well as to ensure the safety of our maritime areas. In this paper, a 3D passive underwater uncooperative target tracking problem for a time-varying non-Gaussian environment is studied. Aiming to overcome the low observability drawback inherent in the passive target tracking problem, a distributed passive underwater buoys observing system is considered and the optimal topology of the distributed measurement system is designed based on the nonlinear system observability analysis theory and the Cramer-Rao lower bound (CRLB) analysis method. Then, considering the unknown underwater environment will lead to time-varying non-Gaussian disturbances for both the target's dynamics and the measurements, the robust optimal nonlinear estimator, namely the adaptive particle filter (APF), is proposed. Based on the Bayesian posterior probability and Monte Carlo techniques, the proposed algorithm utilizes the real-time optimal estimation technique to calculate the complex noise online and tackle the underwater uncooperative target tracking problem. Finally, the proposed algorithm is tested by simulated data and comprehensive comparisons along with detailed discussions that are made to demonstrate the effectiveness of the proposed APF.
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http://dx.doi.org/10.3390/e23070902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307101PMC
July 2021

Fragile X mental retardation protein-regulated proinflammatory cytokine expression in the spinal cord contributes to the pathogenesis of inflammatory pain induced by complete Freund's adjuvant.

J Neurochem 2021 Aug 2. Epub 2021 Aug 2.

Department of Pain Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Studies have verified that Fragile X mental retardation protein (FMRP), an RNA-binding protein, plays a potential role in the pathogenesis of formalin- and (RS)-3,5-dihydroxyphenylglycine-induced abnormal pain sensations. However, the role of FMRP in inflammatory pain has not been reported. Here, we showed an increase in FMRP expression in the spinal dorsal horn (SDH) in a rat model of inflammatory pain induced by complete Freund's adjuvant (CFA). Double immunofluorescence staining revealed that FMRP was mainly expressed in spinal neurons and colocalized with proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)]. After consecutive intrathecal injection of fragile X mental retardation 1 small interfering RNA for 3 days post-CFA injection, FMRP expression in the SDH was reduced, and CFA-induced hyperalgesia was decreased. In addition, the CFA-induced increase in spinal TNF-α and IL-6 production was significantly suppressed by intrathecal administration of fragile X mental retardation 1 small interfering RNA. Together, these results suggest that FMRP regulates TNF-α and IL-6 levels in the SDH and plays an important role in inflammatory pain.
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http://dx.doi.org/10.1111/jnc.15485DOI Listing
August 2021

Double-Shelled Hollow SiO @N-C Nanofiber Boosts the Lithium Storage Performance of [PMo O ].

Chemistry 2021 Sep 13;27(53):13367-13375. Epub 2021 Aug 13.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

Polyoxometalates (POMs)-based materials, with high theoretical capacities and abundant reversible multi-electron redox properties, are considered as promising candidates in lithium-ion storage. However, the poor electronic conductivity, low specific surface area and high solubility in the electrolyte limited their practical applications. Herein, a double-shelled hollow PMo -SiO @N-C nanofiber (PMo -SiO @N-C, where PMo is [PMo O ] , N-C is nitrogen-doped carbon) was fabricated for the first time by combining coaxial electrospinning technique, thermal treatment and electrostatic adsorption. As an anode material for LIBs, the PMo -SiO @N-C delivered an excellent specific capacity of 1641 mA h g after 1000 cycles under 2 A g . The excellent electrochemical performance benefited from the unique double-shelled hollow structure of the material, in which the outermost N-C shell cannot only hinder the agglomeration of PMo , but also improve its electronic conductivity. The SiO inner shell can efficiently avoid the loss of active components. The hollow structure can buffer the volume expansion and accelerate Li diffusion during lithiation/delithiation process. Moreover, PMo can greatly reduce charge-resistance and facilitate electron transfer of the entire composites, as evidenced by the EIS kinetics study and lithium-ion diffusion analysis. This work paves the way for the fabrication of novel POM-based LIBs anode materials with excellent lithium storage performance.
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http://dx.doi.org/10.1002/chem.202101638DOI Listing
September 2021

Developing a Novel Method for Estimating the Speed of Sound in Biodiesel Known as Grey Wolf Optimizer Support Vector Machine Algorithm.

Biomed Res Int 2021 2;2021:5368987. Epub 2021 Jul 2.

Department of Educational Science, Payame Noor University, Damghan, Iran.

In the current study, our goal was to obtain a robust model to predict the speed of sound in biodiesel. For this purpose, an extensive databank has been extracted from previously published papers. Then, a Support Vector Machine (SVM) has been optimized by Grey Wolf Optimization (GWO) method to analyze these data and determine the correlation between speed of sound in biodiesel and its related properties including pressure, temperature, molecular weight, and normal melting point. The results were very satisfactory because the values of statistical parameters and RMSE were obtained 1 and 1.4024, respectively. Here, this is the first time that the sensitivity analysis is used to estimate this target value. This analysis shows that the pressure widely affects the output values with relevancy factor 87.92. Also, our proposed method is highly accurate than other machine learning methods used in papers employed for this objective.
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http://dx.doi.org/10.1155/2021/5368987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270713PMC
September 2021

Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling.

J Inflamm Res 2021 16;14:3393-3403. Epub 2021 Jul 16.

Department of Orthopedics, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong Province, People's Republic of China.

Introduction: Quercetin was recently reported to help protect against osteoarthritis (OA) progression, but the molecular mechanism for that protective affect remains unclear.

Methods: Here, OA model rats were intraperitoneally injected with quercetin, and the severity of cartilage damage in the rats was evaluated by H&E, Safranin O, and Toluidine blue, as well as by using the Osteoarthritis Research Society International (OARSI) Scoring System. Additionally, rat chondrocytes were treated with quercetin and then stimulated with IL-1β. The levels of pro-inflammatory cytokines (IL-1β, IL-18, and TNF-α) were detected by ELISA.Cell apoptosis was evaluated by flow cytometry and Hoechst staining. ROS levels were measured using a DCFH-DA probe. Protein expression was evaluated by Western blotting, immunohistochemical staining, and immunofluorescence.

Results: Our data showed that quercetin attenuated the degeneration and erosion of articular cartilage, suppressed inflammation and apoptosis, and downregulated the levels of IRAK1, NLRP3, and caspase-3 expression. In vitro data showed that overexpression of NLRP3 could reverse the suppressive effect of quercetin on IL-1β-induced rat chondrocyte injuries. Importantly, rescue experiments confirmed that quercetin inhibited IL-1β-induced rat chondrocyte injuries in vitro by suppressing the IRAK1/NLRP3 signaling pathway.

Conclusion: Our study indicated that quercetin inhibits IL-1β-induced inflammation and cartilage degradation by suppressing the IRAK1/NLRP3 signaling pathway.
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http://dx.doi.org/10.2147/JIR.S311924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291879PMC
July 2021

Room-temperature controllable synthesis of hierarchically flower-like hollow covalent organic frameworks for brain natriuretic peptide enrichment.

Chem Commun (Camb) 2021 Jul;57(60):7362-7365

Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.

A facile strategy was introduced for room-temperature controllable synthesis of hierarchically flower-like hollow COFs (FHF-COFs). Furthermore, the universality for synthesis of the HFH-COFs was validated by altering the building units. Inspired by the unique morphology, extremely large surface area and good chemical stability, HFH-COFs could serve as an attractive adsorption probe by loading with gold nanoparticles and be applied to enrichment of brain natriuretic peptide from human serum. This work opens up a whole new approach for controllable synthesis of the HFH-COFs at room temperature and expands the application of COFs as a promising enrichment probe for complex biological samples.
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http://dx.doi.org/10.1039/d1cc02646aDOI Listing
July 2021

Thiol functionalized covalent organic framework for highly selective enrichment and detection of mercury by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Analyst 2021 May;146(9):2991-2997

Partner State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong, SAR, P. R. China.

A spherical thiol-functionalized covalent organic framework (COF-SH) was designed via a facile thiol-yne click reaction of a alkynyl-terminated COF and pentaerythritol tetra(3-mercaptopropionate). The COF-SH was explored as a new adsorbent for the selective enrichment of Hg2+. The as-prepared COF-SH exhibited a uniform mesoporous structure, a high abundance of binding sites, and good chemical stability, which endow it with great performance for the adsorption of Hg2+ and its corresponding maximum adsorption capacity was up to 617.3 mg g-1. Furthermore, the adsorption behavior of Hg2+ on the COF-SH wasin good agreement with the Langmuir and pseudo-second-order models. The influences of adsorbent dosage, pH, selectivity, and reusability of the COF-SH on Hg2+ adsorption were also investigated. Besides this, the COF-SH showed high selectivity towards Hg2+ even in the presence of a high concentration of K+, Na+, Ca2+, Mg2+ and Zn2+ metal ions. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), the corresponding limit of detection (LOD) of Hg2+ was determined at very low concentrations of 80 pg mL-1 (equal to 396 amoL μL-1). In addition, the COF-SH was successfully applied to rapidly enrich and sensitively detect Hg2+ in industrial sewage, with recoveries in the range of 101.8-103.4%, demonstrating the promising potential of COF-SH as an effective adsorbent for use in environmental sample pretreatment.
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http://dx.doi.org/10.1039/d1an00282aDOI Listing
May 2021

Nitric oxide sensing revisited.

Trends Plant Sci 2021 09 16;26(9):885-897. Epub 2021 Apr 16.

Department of Chemistry, Biology, and Biotechnology, University of Perugia, I-06121 Perugia, Italy.

Nitric oxide (NO) sensing is an ancient trait enabled by hemoproteins harboring a highly conserved Heme-Nitric oxide/OXygen (H-NOX) domain that operates throughout bacteria, fungi, and animal kingdoms including in humans, but that has long thought to be absent in plants. Recently, H-NOX-containing plant hemoproteins mediating crucial NO-dependent responses such as stomatal closure and pollen tube guidance have been reported. There are indications that the detection method that led to these discoveries will uncover many more heme-based NO sensors that operate as regulatory sites in complex proteins. Their characterizations will in turn offer a much more complete picture of plant NO responses at both the molecular and systems level.
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http://dx.doi.org/10.1016/j.tplants.2021.03.009DOI Listing
September 2021

One-pot room-temperature synthesis of covalent organic framework-coated superhydrophobic sponges for highly efficient oil-water separation.

J Hazard Mater 2021 Jun 21;411:125190. Epub 2021 Jan 21.

Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address:

Frequent oil-spill accidents not only cause serious and long-term damage to marine ecosystems, but also lead to a huge loss of valuable natural resources. To lighten the environmental pollution of oil spills as quickly as possible, an efficient and environment-friendly approach for oil-water separation is highly desirable. Herein, a facile one-pot room-temperature approach was developed for large-scale fabrication of covalent organic framework-coated superhydrophobic sponges ([email protected]). The as-prepared [email protected] possessed many superior properties, including superhydrophobicity with the water contact angle of approximately 154.3°, large specific surface area (153.059 m/g), high porosity of the network structures, as well as good mechanical and chemical stability. Taking the aformentioned advantages together, the superhydrophobic sponges showed ultra-high adsorption capacity for oil and various organic solvents. In comparision with its own weight, the adsorption amount of the [email protected] for silicone oil was up to 150 times and for toluene was 125 times, respectively. Furthermore, the superhydrophobic sponges also showed fast and highly efficient oil-water separation, outstanding flame retardancy and recyclability. In addition, the [email protected] were successfully applied to the high-efficiency removal of oil suspension from industrial waste water, firmly confirming their application prospect in industrial wastewater treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125190DOI Listing
June 2021

Social deprivation and secondhand smoke exposure among urban male residents: A nationwide study in China.

Tob Induc Dis 2021 22;19:21. Epub 2021 Mar 22.

Department of Epidemiology, School of Public Health, West Virginia University, Morgantown, United States.

Introduction: Social deprivation is a known determinant of health and related behaviors. Many studies have linked socioeconomic factors to secondhand smoke (SHS) exposure. However, no studies have examined the relationship between social deprivation and SHS exposure. This study examined whether contextual social deprivation - variously based on living in a house without a car, that was overcrowded, or had an unemployed member (s) - had an independent association with SHS exposure at both individual and regional levels among Chinese residents.

Methods: A cross-sectional multistage sampling design was utilized to interview subjects from 6 selected cities in China. A standardized questionnaire selected sociodemographic characteristics, contextual social deprivation and SHS exposure. Multilevel logistic regression models were used to assess the association between social deprivation and SHS exposure.

Results: A total of 5782 valid questionnaires were collected in this study. Among 2930 non-smokers, the SHS exposure prevalence was 21.9% (95% CI: 19.5- 24.30). Multilevel logistic regression showed a negative association between household income, regional GDP, and SHS exposure, respectively, and positive associations between contextual social deprivation and SHS exposure.

Conclusions: Findings support the central proposition that contextual social deprivation must be factored into SHS exposure messages. Our research underscores the importance of reducing health inequality in controlling SHS exposure.
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http://dx.doi.org/10.18332/tid/132290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983223PMC
March 2021

Convex compressive beamforming with nonconvex sparse regularization.

J Acoust Soc Am 2021 Feb;149(2):1125

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an 710072, China.

The convex sparse penalty based compressive beamforming technique can achieve robust high resolution in direction-of-arrival (DOA) estimation tasks, but it often leads to an insufficient sparsity-inducing problem due to its convex loose approximation to ideal ℓ nonconvex penalty. On the contrary, the nonconvex sparse penalty can tightly approximate ℓ penalty to effectively enhance DOA estimation accuracy, but it incurs an initialization sensitivity problem due to its multiple local minimas. Leveraging their individual advantages, a minimax-concave penalty (MCP) regularized DOA estimation algorithm is proposed to achieve a maximally sparse level while maintaining the convex property of the overall objective function. Moreover, an accelerated block gradient descent-ascent algorithm with convergence guarantee is developed to rapidly achieve its one optimal point. Simulation results demonstrate that MCP penalty improves DOA estimation accuracy compared with popular sparse compressive beamforming techniques in strong noise scenarios and weak source confirmation. Ocean experimental results also validate that it retains more stable DOA estimation accuracy and incurs less artificial interferences.
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http://dx.doi.org/10.1121/10.0003373DOI Listing
February 2021

Facile fabrication of magnetic covalent organic frameworks and their application in selective enrichment of polychlorinated naphthalenes from fine particulate matter.

Mikrochim Acta 2021 02 18;188(3):91. Epub 2021 Feb 18.

Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, Fujian, China.

Magnetic covalent organic frameworks ([email protected]) were synthesized via a one-pot process in which magnetic nanoparticles ([email protected]) served as a magnetic core and 2,4,6-trihydroxy-1,3,5-benzenetricarbaldehyde (TP) and 2,2',5,5'-tetrachlorobenzidine (PCl) as two building blocks to form a shell. The as-prepared [email protected] nanoparticles have superior features, including large surface area (186.5 m g), high porosity, strong magnetic responsiveness (42.6 emu g), high chlorine content, and outstanding thermal stability, which make them an ideal adsorbent for highly selective enrichment of polychlorinated naphthalenes (PCNs). Combining with atmospheric pressure gas chromatography tandem mass spectrometry (APGC-MS/MS), a simple analytical method of [email protected] magnetic solid-phase extraction (MSPE)-APGC-MS/MS was developed, which exhibited good linearity (r ≥ 0.9991) for eight PCNs in the concentration range 0.1-100 ng L. Moreover, low detection limits (0.005-0.325 ng L), high enrichment factors (46.62-81.97-fold), and good relative standard deviations (RSDs) of inter-day (n = 3, 1.64 to 7.44%) and day-to-day (n = 3, 2.62 to 8.23%) were achieved. This method was successfully applied to the selective enrichment of PCNs in fine particulate matter (PM) samples, and ultra-trace PCNs were found in the range 1.56-3.75 ng kg with satisfactory recoveries (93.11-105.81%). The successful application demonstrated the great potential of [email protected] nanoparticles as an adsorbent for enrichment of halogenated compounds. Schematic presented one-pot synthesis of magnetic covalent organic framework nanocomposites ([email protected]) and their application in the selective enrichment of PCNs from PM prior to APGC-MS/MS analysis.
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http://dx.doi.org/10.1007/s00604-021-04750-zDOI Listing
February 2021

Effect of antioxidants coenzyme Q10 and β-carotene on the cytotoxicity of vemurafenib against human malignant melanoma.

Oncol Lett 2021 Mar 14;21(3):208. Epub 2021 Jan 14.

School of Natural Sciences, College of Science and Technology, Wenzhou Kean University, Wenzhou, Zhejiang 325035, P.R. China.

Melanoma is a type of highly invasive skin cancer derived from melanocytes with poor prognosis. Vemurafenib (PLX4032) is a clinically approved targeted therapeutic for BRAF mutant melanoma that has a high therapeutic response rate and significantly prolongs the overall survival time of patients with melanoma. Antioxidants have been widely used as supplements for cancer prevention and for decreasing the side effects of cancer therapy. However, antioxidants can also protect cancer cells from oxidative stress and promote cancer growth and progression. The present study aimed to examine the effect of the antioxidants coenzyme Q10 (CoQ10) and β-carotene on melanoma cell growth and invasiveness and on the cytotoxicity of vemurafenib against both vemurafenib-sensitive (SK-MEL-28) and vemurafenib-resistant (A2058) human malignant melanoma cell lines. MTS assay and wound-healing assay demonstrated that CoQ10 alone significantly reduced the viability and migration of melanoma cells, respectively, and synergistically worked with vemurafenib to decrease the viability and migration of human melanoma cells. In contrast, MTS assay and flow cytometry revealed that β-carotene alone did not affect the viability and apoptosis induction of melanoma cells; however, it inhibited cell migration and invasiveness. Wound-healing and Transwell assay demonstrated that β-carotene alleviated the cytotoxicity of vemurafenib and mitigated the inhibitory effect of vemurafenib on cell migration and invasion. Both CoQ10 and β-carotene protected melanoma cells from undergoing apoptosis induced by vemurafenib. Immunoblotting demonstrated that β-carotene at physiological concentration worked synergistically with vemurafenib to suppress the Ras-Raf-Mek-Erk intracellular signaling pathway. The present study aimed to add to the evidence of the effects of CoQ10 and β-carotene on the antimelanoma effects of vemurafenib.
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http://dx.doi.org/10.3892/ol.2021.12469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816282PMC
March 2021

Exposure to electronic screen before nocturnal sleep increases the risk of hypertensive disorders of pregnancy: A case-control study.

J Obstet Gynaecol Res 2021 Feb 3;47(2):698-704. Epub 2020 Dec 3.

Department of Prevention and Health, Liyang People's Hospital, Liyang, China.

Aim: Previous studies indicated that excessive screen time was associated with hypertension in children and adolescents. In our hospital, pregnant women tended to spend a lot of time on electronic devices like smartphones. We aimed to explore the relationship between the screen time and hypertensive disorders of pregnancy (HDP).

Methods: A case-control study was conducted from November 2019 to May 2020. A total of 160 women with HDP and 197 healthy controls who gave birth to children in the same hospital were recruited and information was recorded by questionnaires. Multivariate analysis was conducted to assess the effect of screen time on HDP.

Results: The results showed that, exposure to electronic screen before nocturnal sleep in cases was significantly longer than that in controls (P = 0.011, odds ratio = 1.50). Smartphones (and/or tablet computers) used only for entertainment also significantly increased the susceptibility to HDP (P < 0.001, odds ratio = 2.84). Other related factors were following: work experience during pregnancy (P = 0.034, odds ratio = 0.53), history of diabetes mellitus (P = 0.013, odds ratio = 2.55), history of family hypertension (P < 0.001, odds ratio = 3.81), body mass index of pre-pregnancy (>25 kg/m ) (P < 0.001, odds ratio = 6.16).

Conclusion: In conclusion, long exposure to electronic screen before nocturnal sleep and the smartphones usage for only entertainment may be associated with the susceptibility to HDP.
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http://dx.doi.org/10.1111/jog.14588DOI Listing
February 2021

Identification of potential nitric oxide-sensing proteins using the H-NOX motif.

Mol Plant 2021 02 27;14(2):195-197. Epub 2020 Nov 27.

Department of Chemistry, Biology & Biotechnology, University of Perugia, 06121 Perugia, Italy.

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http://dx.doi.org/10.1016/j.molp.2020.11.015DOI Listing
February 2021

Development and validation of a fourteen- innate immunity-related gene pairs signature for predicting prognosis head and neck squamous cell carcinoma.

BMC Cancer 2020 Oct 20;20(1):1015. Epub 2020 Oct 20.

Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Chongqing Medical University, No 1. Youyi Road, Yuzhong District, Chongqing, 400016, China.

Background: Immune-related genes is closely related to the occurrence and prognosis of head and neck squamous cell carcinoma (HNSCC). At the same time, immune-related genes have great potential as prognostic markers in many types of cancer. The prognosis of HNSCC is still poor currently, and it may be effective to predict the clinical outcome of HNSCC by immunogenic analysis.

Methods: RNASeq and clinical follow-up information were downloaded from The Cancer Genome Atlas (TCGA), the MINiML format GSE65858 chip expression data was downloaded from NCBI, and immune-related genes was downloaded from the InnateDB database. Immune-related genes in 519 HNSC patients were integrated from TCGA dataset. By using multivariate COX analysis and Lasso regression, robust immune-related gene pairs (IRGPs) that predict clinical outcomes of HNSCC were identified. Finally, a risk prognostic model related to immune gene pair was established and verified by clinical features, test sets and GEO external validation set.

Results: A total of 699 IRGPs were significantly correlated with the prognosis of HNSCC patients. Fourteen robust IRGPs were finally obtained by Lasso regression and a prognostic risk prediction model was constructed. Risk score of each sample were calculated based on Risk models and divided into the high-risk group (Risk-H) and low Risk group (Risk-L). Risk models were able to stratify the risk in patients with TNM Stage, Age, gender, and smoking history, and the AUC > 0.65 in training set and test set, shows that 14-IRGPs signature in patients with HNSCC has excellent classification performance. In addition, 14-IRGPs had the highest average C index compared with the prognostic characteristics and T, N, and Age of the 3 previously reported HNSCC.

Conclusion: This study constructed 14-IRGPs as a novel prognostic marker for predicting survival in HNSCC patients.
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http://dx.doi.org/10.1186/s12885-020-07489-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574345PMC
October 2020

Arbitrary-order superdirectivity of circular sensor arrays.

J Acoust Soc Am 2020 Sep;148(3):1425

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

Superdirectivity can yield large directivity using a small array aperture, but it suffers from high sensitivity to random errors. In a previously proposed model, robust Nth-order superdirectivity was obtained by combining low-order superdirectivity sub-solutions with orders from 0 to N. However, the orders N were exclusively positive integers, which may lead to an over-improved robustness with an excessively degraded directivity. This paper presents an arbitrary-order superdirectivity model of circular sensor arrays, which can achieve a more flexible compromise between directivity and robustness than the previous model. A coefficient η is weighted at the (N + 1)th-order entry of the optimal weighting vector and the new weighting vector will consist of the 0th- to Nth-order and the weighted (N + 1)th-order entries. The beampattern, the directivity factor, and the error sensitivity function can then be expressed as functions of the coefficient η. Moreover, broadband superdirective beampatterns with a desired directivity factor or error sensitivity function can be readily synthesized using the closed-form solutions derived from the arbitrary-order superdirectivity model. Simulations and experimental results show that the proposed model can easily synthesize superdirective beampatterns with arbitrary orders, and it is more flexible than the previous integer-order model. It is also demonstrated that the performance of the proposed method is similar to that of the optimization-based method, but it uses a simple closed-form weighting vector rather than a numerical algorithm.
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http://dx.doi.org/10.1121/10.0001937DOI Listing
September 2020

Downregulation of PER2 Promotes Tumor Progression by Enhancing Glycolysis via the Phosphatidylinositol 3-Kinase/Protein Kinase B Pathway in Oral Squamous Cell Carcinoma.

J Oral Maxillofac Surg 2020 Oct 1;78(10):1780.e1-1780.e14. Epub 2020 Jun 1.

Professor, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: PER2 gene expression is downregulated in oral squamous cell carcinoma (OSCC) and may have a pivotal role in tumor suppression. However, the biological function and mechanism of action of PER2 in OSCC remain unclear. In this study, the biological functions and anticancer mechanisms of PER2 in OSCC were investigated.

Materials And Methods: Both stably overexpressed and silenced PER2 OSCC cells were established as an experimental group; empty vector lentivirus and scramble short hairpin RNA lentivirus transfected-cells, as negative control groups; and untreated OSCC cells, as a blank group. Cell proliferation, apoptosis, and glycolysis potential assays were conducted. In addition, the expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), phosphorylation of protein kinase B, hexokinase 2 (HK2), pyruvate kinase M (PKM2), and lactate dehydrogenase A (LDHA) was quantified by real-time quantitative polymerase chain reaction and Western blotting. Rescue experiments were performed by the addition of AKT activators in the overexpressed cell line and by the addition of glycolysis inhibitor in the silenced cell line. These findings were verified in vivo using stably transfected OSCC cells overexpressing PER2 implanted in nude mice.

Results: PER2 overexpression significantly inhibited OSCC cell proliferation and glycolysis, promoted cell apoptosis, and reduced the expression of PI3K, phosphorylation of protein kinase B, HK2, PKM2, and LDHA. The converse was observed in PER2-silenced OSCC cells. After the addition of AKT activator to cultures of PER2-overexpressed OSCC cells, reduced glucose uptake, lactic acid production, and cell proliferation, combined with increased apoptosis, were substantially reversed. In addition, after the addition of HK2 inhibitor to PER2-silenced OSCC cells to inhibit glycolysis, the reduction in apoptosis and increased proliferation were significantly countermanded. Tumorigenesis experiments in vivo also confirmed that PER2 overexpression suppressed OSCC growth and decreased the expression of HK2, PKM2, and LDHA.

Conclusions: PER2 heightened glycolysis via the PI3K/AKT pathway, heightened cell proliferation and inhibited apoptosis via glycolysis, thereby promoting the development of OSCC.
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http://dx.doi.org/10.1016/j.joms.2020.05.035DOI Listing
October 2020

Genetic Polymorphisms of Cytokines Might Affect Postoperative Sufentanil Dosage for Analgesia in Patients.

J Pain Res 2020 16;13:1461-1470. Epub 2020 Jun 16.

Department of Pain Medicine, The First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, Zhejiang 310003, People's Republic of China.

Objective: To explore the effect of genetic polymorphisms of cytokines on the dosage of sufentanil for patient-controlled intravenous analgesia (PCIA) after radical lung cancer surgery.

Methods: A total of 100 patients, aged 18 years and above, with ASA grade Ⅰ-Ⅱ and body mass index (BMI) 18.5 to 30, and who were scheduled for radical lung cancer surgery under total intravenous anaesthesia with PCIA of sufentanil from September 2015 to March 2016, were selected. DNA was collected from peripheral blood samples before surgery, and the iMLDRTM multiple single-nucleotide polymorphism typing kit was used to detect 16 related single-nucleotide polymorphism (SNP) sites of interleukin-1A (IL-1A), interleukin-1β (IL-1β), interleukin-1RN (IL-1RN), interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 (CXCL8), interleukin-10 (IL-10), tumour necrosis factor (TNF), nuclear factor kappa-B1 (NFκB1), REL (REL proto-oncogene, NF-kB subunit), and nuclear factor kappa-B inhibitor alpha (NFκBIA). The general characteristics of patients, surgery and anaesthesia data, postoperative resting VAS pain scores, postoperative opioid dosages of sufentanil for PCIA and opioid-related adverse events were recorded. The effects of the examined genetic polymorphisms of the cytokines on the dosage of sufentanil were analysed.

Results: Eight of 100 patients withdrew for various reasons, and, eventually, 92 patients were included. The patients' resting visual analogue scale (VAS) scores at 24 h, 48 h, and 72 h after surgery were 2.3 ± 1.2, 2.0 ± 0.9, and 1.9 ± 1.0, respectively. The total amounts of sufentanil used were 34.7 ± 10.5 μg, 65.2 ± 13.7 μg, and 94.7 ± 11.6 μg, respectively. We found that the TT genotype of NFκBIA rs696 had higher PCIA sufentanil dosages than the CC genotype and the CT genotype at 48-72 h postoperation (p=0.023, p=0.025, respectively).

Conclusion: The genetic polymorphisms of the cytokine NFκBIA rs696 might affect the dosage of sufentanil for PCIA after radical lung cancer surgery. The specific mechanism needs further study.
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http://dx.doi.org/10.2147/JPR.S250174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305826PMC
June 2020

Role of fragile X mental retardation protein in chronic pain.

Mol Pain 2020 Jan-Dec;16:1744806920928619

Department of Pain Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Chronic pain has detrimental effects on one's quality of life. However, its treatment options are very limited, and its underlying pathogenesis remains unclear. Recent research has suggested that fragile X mental retardation protein is involved in the development of chronic pain, making it a potential target for prevention and treatment. The current review of literature will examine the function of fragile X mental retardation protein and its associated pathways, through which we hope to gain insight into how fragile X mental retardation protein may contribute to nociceptive sensitization and chronic pain.
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http://dx.doi.org/10.1177/1744806920928619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273537PMC
June 2021

Source localization in the deep ocean using a convolutional neural network.

J Acoust Soc Am 2020 04;147(4):EL314

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an 710072, China.

In deep-sea source localization, some of the existing methods only estimate the source range, while the others produce large errors in distance estimation when estimating both the range and depth. Here, a convolutional neural network-based method with high accuracy is introduced, in which the source localization problem is solved as a regression problem. The proposed neural network is trained by a normalized acoustic matrix and used to predict the source position. Experimental data from the western Pacific indicate that this method performs satisfactorily: the mean absolute percentage error of the range is 2.10%, while that of the depth is 3.08%.
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http://dx.doi.org/10.1121/10.0001020DOI Listing
April 2020

Deficiency of platelet adhesion molecule CD226 causes megakaryocyte development and platelet hyperactivity.

FASEB J 2020 05 5;34(5):6871-6887. Epub 2020 Apr 5.

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China.

This study used constitutive CD226 gene knockout (KO) mice as a model to investigate the functions and mechanisms of CD226 in megakaryocyte (MK) maturation and platelet activation. Although CD226 deficiency did not cause MK polyploidization or platelet granule abnormalities, increased MK counts were detected in the femora bone marrow (BM) and spleen of CD226 KO mice. Particularly, CD226 KO mice have a more extensive membrane system in MKs and platelets than wild-type (WT) mice. We also demonstrated that CD226 KO mice displayed increased platelet counts, shortened bleeding time, and enhanced platelet aggregation. CD226 KO platelets had an increased mature platelet ratio compared to the control platelets. In addition, the observed reduction in bleeding time may be due to decreased nitric oxide (NO) production in the platelets. Platelet-specific CD226-deficient mice showed similar increased MK counts, shortened bleeding time, enhanced platelet aggregation, and decreased NO production in platelets. Furthermore, we performed middle cerebral artery occlusion-reperfusion surgery on WT and CD226 KO mice to explore the potential effect of CD226 on acute ischemia-reperfusion injury; the results revealed that CD226 deficiency led to significantly increased infarct area. Thus, CD226 is a promising candidate for the treatment of thrombotic disorders.
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http://dx.doi.org/10.1096/fj.201902142RDOI Listing
May 2020

Loss of the clock gene Per1 promotes oral squamous cell carcinoma progression via the AKT/mTOR pathway.

Cancer Sci 2020 May 18;111(5):1542-1554. Epub 2020 Mar 18.

Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Current studies have shown that the clock gene Period 1 (Per1) is downregulated in various tumors and plays an important role in promoting tumor progression. However, the biological functions and mechanism of Per1 in tumors remain largely unknown. In this study, 86 specimens of oral squamous cell carcinoma (OSCC) tissues and adjacent noncancerous tissues were collected to determine the Per1 expression level and the clinical significance of Per1 expression. Per1 was stably inhibited or overexpressed in OSCC cells to investigate its function and mechanism in vitro and in vivo. We found that Per1 was remarkably downregulated in OSCC and that low Per1 expression was significantly associated with TNM clinical stage and poor prognosis of OSCC patients. Per1 overexpression in SCC15 OSCC cells (Per1-OE SCC15 cells) significantly promoted autophagy and apoptosis while inhibiting proliferation and the AKT/mTOR pathway. However, the results obtained in Per1-silenced TSCCA OSCC cells were opposite those obtained in Per1-OE SCC15 cells. After addition of the AKT activator SC79 to Per1-OE SCC15 cells, the increased autophagy and apoptosis as well as decreased proliferation were remarkably rescued. Furthermore, increased apoptosis was significantly rescued in Per1-OE SCC15 cells treated with the autophagy inhibitor autophinib. In vivo tumorigenicity assays also confirmed that Per1 overexpression suppressed tumor growth. Taken together, our findings demonstrate for the first time that Per1 promotes OSCC progression by inhibiting autophagy-mediated cell apoptosis and enhancing cell proliferation in an AKT/mTOR pathway-dependent manner, and Per1 could be used as a valuable therapeutic target for OSCC.
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http://dx.doi.org/10.1111/cas.14362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226219PMC
May 2020

A cluster-based direct source localization approach for large-aperture horizontal line arrays.

J Acoust Soc Am 2020 Jan;147(1):EL50

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an, 710072,

In this paper, the problem of source localization using one-step direct position determination (DPD) approaches with large-aperture horizontal line arrays is considered. In practice, it may be difficult to keep the array configuration and overlarge inter-element spacing may cause the split beam problem when decentralized DPD approaches are applied. A cluster-based coherent MUSIC DPD approach is proposed to handle the unfavourable array configuration. The large-aperture array is divided into several small-aperture arrays, which form a coherent cluster to keep the largest covariance lag unchanged. Experimental results with the SWellEx96 data demonstrate the performance gain achieved by cluster-based coherent processing.
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http://dx.doi.org/10.1121/10.0000590DOI Listing
January 2020

Spinal NF-kB upregulation contributes to hyperalgesia in a rat model of advanced osteoarthritis.

Mol Pain 2020 Jan-Dec;16:1744806920905691

Department of Pain Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

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http://dx.doi.org/10.1177/1744806920905691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040927PMC
February 2021

[Recent advances in graphitic carbon nitride materials for sample pretreatment].

Se Pu 2020 Jan;38(1):28-35

College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.

As a new type of non-metallic material, graphite carbon nitride (g-CN) has attracted increasing attention due to its inherent advantages such as simple preparation, excellent thermal and chemical stability, as well as good biocompatibility and non-toxicity. Nowadays, g-CN is widely used in electrocatalysis, photocatalysis, biological imaging, and so on. Because of its large specific surface area, -electron-rich structure, and hydrophobic properties, g-CN is considered an ideal candidate material for sample pretreatment. In this work, g-CN and its composites as potential sorbents for solid-phase extraction, dispersive solid-phase extraction, magnetic solid-phase extraction, and solid-phase microextraction are reviewed, and the future trends and prospective are discussed.
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http://dx.doi.org/10.3724/SP.J.1123.2019.06032DOI Listing
January 2020

Source localization by matching sound intensity with a vertical array in the deep ocean.

J Acoust Soc Am 2019 Dec;146(6):EL477

First Institute of Oceanography, Ministry of Natural Resources of China, Qingdao, 266061,

The characteristics of the reliable acoustic path (RAP) have been widely used to estimate source depth in the deep ocean. Here, a passive broadband source localization method that estimates both depth and range using the interference of the broadband signal with a non-synchronous vertical array in the RAP is demonstrated. The acoustic intensity distribution versus frequency and depth, which includes interference characteristics, is calculated from non-synchronous vertical array data and used to locate the source. Simulations and an experiment conducted in the western Pacific show that this method can successfully locate a broadband submerged source in the range of 10-30 km.
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http://dx.doi.org/10.1121/1.5139191DOI Listing
December 2019
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