Publications by authors named "Yingying Wang"

690 Publications

DNA barcode to trace the development and differentiation of cord blood stem cells (Review).

Mol Med Rep 2021 Dec 13;24(6). Epub 2021 Oct 13.

Laboratory of Oncology, Center for Molecular Medicine, School of Basic Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, P.R. China.

Umbilical cord blood transplantation was first reported in 1980. Since then, additional research has indicated that umbilical cord blood stem cells (UCBSCs) have various advantages, such as multi‑lineage differentiation potential and potent renewal activity, which may be induced to promote their differentiation into a variety of seed cells for tissue engineering and the treatment of clinical and metabolic diseases. Recent studies suggested that UCBSCs are able to differentiate into nerve cells, chondrocytes, hepatocyte‑like cells, fat cells and osteoblasts. The culture of UCBSCs has developed from feeder‑layer to feeder‑free culture systems. The classical techniques of cell labeling and tracing by gene transfection and fluorescent dye and nucleic acid analogs have evolved to DNA barcode technology mediated by transposon/retrovirus, cyclization recombination‑recombinase and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‑associated protein 9 strategies. DNA barcoding for cell development tracing has advanced to include single cells and single nucleic acid mutations. In the present study, the latest research findings on the development and differentiation, culture techniques and labeling and tracing of UCBSCs are reviewed. The present study may increase the current understanding of UCBSC biology and its clinical applications.
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http://dx.doi.org/10.3892/mmr.2021.12489DOI Listing
December 2021

The Combination of Radiotherapy With Immunotherapy and Potential Predictive Biomarkers for Treatment of Non-Small Cell Lung Cancer Patients.

Front Immunol 2021 21;12:723609. Epub 2021 Sep 21.

Department of Radiation Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Radiotherapy is an effective local treatment modality of NSCLC. Its capabilities of eliminating tumor cells by inducing double strand DNA (dsDNA) damage and modulating anti-tumor immune response in irradiated and nonirradiated sites have been elucidated. The novel ICIs therapy has brought hope to patients resistant to traditional treatment methods, including radiotherapy. The integration of radiotherapy with immunotherapy has shown improved efficacy to control tumor progression and prolong survival in NSCLC. In this context, biomarkers that help choose the most effective treatment modality for individuals and avoid unnecessary toxicities caused by ineffective treatment are urgently needed. This article summarized the effects of radiation in the tumor immune microenvironment and the mechanisms involved. Outcomes of multiple clinical trials investigating immuno-radiotherapy were also discussed here. Furthermore, we outlined the emerging biomarkers for the efficacy of PD-1/PD-L1 blockades and radiation therapy and discussed their predictive value in NSCLC.
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http://dx.doi.org/10.3389/fimmu.2021.723609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490639PMC
September 2021

Fiber-enhanced Raman spectroscopy for highly sensitive H and SO sensing with a hollow-core anti-resonant fiber.

Opt Express 2021 Sep;29(20):32296-32311

An innovative fiber-enhanced Raman gas sensing system with a hollow-core anti-resonant fiber is introduced. Two iris diaphragms are implemented for spatial filtering, and a reflecting mirror is attached to one fiber end that provides a highly improved Raman signal enhancement over 2.9 times than the typical bare fiber system. The analytical performance for multigas compositions is thoroughly demonstrated by recording the Raman spectra of carbon dioxide (CO), oxygen (O), nitrogen (N), hydrogen (H), and sulfur dioxide (SO) with limits of detection down to low-ppm levels as well as a long-term instability < 1.05%. The excellent linear relationship between Raman signal intensity (peak height) and gas concentrations indicates a promising potential for accurate quantification.
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http://dx.doi.org/10.1364/OE.437693DOI Listing
September 2021

Identification of a novel A allele with c.792_794delCTA in a Chinese individual.

Transfusion 2021 Sep 29. Epub 2021 Sep 29.

Department of Blood Transfusion, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, China.

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http://dx.doi.org/10.1111/trf.16686DOI Listing
September 2021

Elevated LINC01232 is associated with poor prognosis and HBV infection in hepatocellular carcinoma patients and contributes to tumor progression in vitro.

Clin Res Hepatol Gastroenterol 2021 Sep 25:101813. Epub 2021 Sep 25.

Department of Hepatobiliary Surgery, Weifang People's Hospital, Weifang 261041, Shandong, China. Electronic address:

Background: Hepatocellular carcinoma (HCC) had high prevalence and poor prognosis, and hepatitis B virus (HBV) infection is a major risk factor. The aim of this study is to analyze the role of long intergenic noncoding RNA 01232 (LINC01232) in the prognosis and progression of HCC, and explore the relationship between LINC01232 and HBV infection.

Methods: LINC01232 expression and its prognostic value were firstly analyzed using TCGA database. Quantitative real-time PCR was used to evaluate the expression of LINC01232 in HCC patients and cell lines. Kaplan-Meier curves were used to analyze the relationship between LINC01232 expression and HCC overall survival prognosis. Function-loss in vitro experiments were performed to demonstrate the role of LINC01232 in HCC progression. A luciferase reporter assay and Pearson correlation were used to confirm the relationship between LINC01232 and microRNA (miR)-708-5p in HCC.

Results: The expression of LINC01232 was upregulated in HCC tissues and cell lines, and high LINC01232 was associated with worse overall survival in HCC. LINC01232 reduction inhibited HCC cells proliferation, migration and invasion. LINC01232 expression was significantly correlated with HBV infection and liver cirrhosis, and showed potential to distinguish HBV-infected HCC patients. miR-708-5p, as a HBV-related miRNA, was a potential target of LINC01232, and was negatively correlated with LINC01232 in HCC.

Conclusion: Our findings found that highly expressed LINC01232 may be a biomarker to indicate survival prognosis in HCC patients, especially in HBV-infected cases. In addition, LINC01232 plays as an oncogene in HCC progression, and its function may exert by sponging miR-708-5p.
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http://dx.doi.org/10.1016/j.clinre.2021.101813DOI Listing
September 2021

Adsorption Performance of Amino Functionalized Magnetic Molecular Sieve Adsorbent for Effective Removal of Lead Ion from Aqueous Solution.

Nanomaterials (Basel) 2021 Sep 11;11(9). Epub 2021 Sep 11.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

Lead ion (Pb) has high toxicity and brings great harm to human body. It is very important to find an effective method to address lead ion pollution. In this work, amino functionalized CoFeO/SBA-15 nanocomposite (NH-CoFeO/SBA-15) was prepared for the effective removal of Pb from aqueous solution. The prepared NH-CoFeO/SBA-15 adsorbent was manifested by using scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectrum (FTIR), X-ray powder diffraction (XRD), and Brunauer-Emmett-Teller (BET) analysis. In the meantime, the adsorption conditions, including pH, adsorbent dosage, and adsorption time, were studied. The investigation of adsorption kinetics revealed that the adsorption results conform to the pseudo-first-order kinetic model. The adsorption isotherms research displayed that the adsorption was consistent with the Freundlich model, demonstrating that the adsorption for Pb with the prepared adsorbent was a multimolecular layer adsorption process. In addition, the thermodynamic investigations (Δ < 0, Δ > 0, Δ > 0) demonstrated that the adsorption for Pb with the prepared adsorbent was endothermic and spontaneous. Moreover, the prepared adsorbent showed superior anti-interference performance and reusability, implying the potential application of the adsorbent in actual water treatment. Furthermore, this research may provide a reference and basis for the study of other heavy metal ions.
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http://dx.doi.org/10.3390/nano11092353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467783PMC
September 2021

Measurement of the Total Lung Volume Using an Adjusted Single-Breath Helium Dilution Method in Patients With Obstructive Lung Disease.

Front Med (Lausanne) 2021 8;8:737360. Epub 2021 Sep 8.

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Whole-body plethysmography (WBP) is the gold standard for measuring lung volume, but its clinical application is limited as it requires expensive equipment and is not simple to use. Studies have shown that the single-breath helium dilution (SBHD) method, which is commonly used in clinical practice, significantly underestimates lung volume in patients with obstructive lung disease (OLD). By comparing the differences in lung volume measured using SBHD and WBP, we aimed to establish a correction equation for the SBHD method to determine the total lung volume in patients with OLD of different severities. From 628 patients with OLD simultaneously subjected to SBHD and WBP, 407 patients enrolled between January 2018 and November 2019 were in the training group and 221 enrolled between December 2019 and December 2020 were in the prospective verification cohort. The multiple linear regression equation was used for data in the training group to establish a correction equation for SBHD to determine the total lung volume, and this was validated in the prospective validation cohort. There was a moderate positive correlation between total lung capacity (TLC) determined using the SBHD [TLC (SBHD)] and WBP methods [TLC (WBP)] (r = 0.701; < 0.05), and the differences between TLC (SBHD) and TLC (WBP) (ΔTLC) were related to the severity of obstruction. As the severity of obstruction increased, the TLC was underestimated by the SBHD method. We established the following correction equation: () () = -0.669 + 0.756*()() - 0.047* +0.039* ()-0.009*()(r2 = 0.753 and adjusted r2 = 0.751). Next, we validated this equation in the validation cohort. With the correction equation, no statistical difference was observed between TLC (adjusted SBHD) and TLC (WBP) among the obstruction degree groups ( > 0.05). The SBHD method is correlated with WBP to measure the total lung volume, but the SBHD method presents limitations in determining the total lung volume in patients with obstructive lung disease. Here, we established an effective and reliable correction equation in order to accurately assess the total lung volume of patients with OLD using the SBHD method.
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http://dx.doi.org/10.3389/fmed.2021.737360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455942PMC
September 2021

Bayesian MEG time courses with fMRI priors.

Brain Imaging Behav 2021 Sep 25. Epub 2021 Sep 25.

Pediatric Neuroimaging Research Consortium, Cincinnati Children's Hospital, Cincinnati, OH, 45229, USA.

Magnetoencephalography (MEG) records brain activity with excellent temporal and good spatial resolution, while functional magnetic resonance imaging (fMRI) offers good temporal and excellent spatial resolution. The aim of this study is to implement a Bayesian framework to use fMRI data as spatial priors for MEG inverse solutions. We used simulated MEG data with both evoked and induced activity and experimental MEG data from sixteen participants to examine the effectiveness of using fMRI spatial priors in MEG source reconstruction. For simulated MEG data, incorporating the prior information from fMRI increased the spatial resolution of MEG source reconstruction by 3 mm on average. For experimental MEG data, fMRI spatial information reduced the spurious clusters for evoked activity and showed more left-lateralized activation pattern for induced activity. The use of fMRI spatial priors greatly reduced location error for induced source in MEG data. Our results provide empirical evidence that the use of fMRI spatial priors improves the accuracy of MEG source reconstruction. The combined MEG and fMRI approach can provide neuroimaging data with better spatial and temporal resolutions to add another perspective to our understanding of the neurobiology of language. The potential clinical applications include pre-surgical evaluation of language function for epilepsy patients and evaluation of language network for children with language disorders.
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http://dx.doi.org/10.1007/s11682-021-00550-4DOI Listing
September 2021

Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures.

Ecotoxicol Environ Saf 2021 Dec 21;225:112799. Epub 2021 Sep 21.

Department of Environmental Microbiology, Eawag: Swiss Federal Institute of Aquatic Science and Technology, Dübendorf 8600, Switzerland. Electronic address:

Bacterial inactivation efficiency of chlorine varies with organisms and environmental conditions. The comparability of different samples/studies, especially comparing indigenous bacterial communities with pure cultures, is impeded by inconsistent experimental conditions and analytical methods used in various studies. We tested a novel 96-well plate FCM experimental and automated analytical approach, where bacterial communities and pure cultures were suspended in the same natural water matrix prior to chlorination directly in the plate. We demonstrated the ability to rapidly monitor the efficiency of 32 different combinations of chlorine concentration and time (i.e. chlorine exposure) on bacterial pure cultures and indigenous aquatic communities, which enabled correct comparison of the data from different samples under the exact same experimental conditions. In this study, the 96-well plate automated FCM approach enabled large sets (896) of independent chlorination experiments to be carried out in a short time period. To our knowledge, this is the largest dataset of chlorination experiments which consumed least time (within 18 h after sampling) until now. Staining with SYBR Green I (SG) and SG combined with propidium iodide (SGPI) was used to assess cellular damage during chlorination. The results showed that with the same chlorine exposure, a higher chlorine concentration with a shorter contact time is favorable for inactivation of bacteria. Our research provides a promising framework to compare disinfection characteristics of various microorganism and can be further developed to diagnose effect of antimicrobial products.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112799DOI Listing
December 2021

Digoxin exerts anticancer activity on human nonsmall cell lung cancer cells by blocking PI3K/Akt pathway.

Biosci Rep 2021 Oct;41(10)

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China.

Lung cancer remains the leading cause of cancer mortality because of its metastatic potential and high malignancy. The discovery of new applications for old drugs is a shortcut for cancer therapy. We recently investigated the antitumor effect of digoxin, a well-established drug for treating heart failure, against nonsmall cell lung cancer A549 and H1299 cells. Digoxin inhibited the proliferation and colony-forming ability of the two cell lines and arrested the cell cycle at the G0/G1 phase in A549 cells and the G2/M phase in H1299 cells. Mitochondria-mediated apoptosis was induced in A549 cells but not in H1299 cells after treatment with digoxin. Moreover, digoxin inhibited the migration, invasion, adhesion and epithelial-mesenchymal transition of A549 and H1299 cells. Autophagy was induced in both cell lines after treatment with digoxin, with an increase in autophagosome foci. In addition, digoxin inhibited the phosphorylation of Akt, mTOR and p70S6K, signaling molecules of the PI3K/Akt pathway that are known to be involved in tumor cell survival, proliferation, metastasis and autophagy. Our findings suggest that digoxin has the potential to be used for therapy for human nonsmall cell lung cancer, but further evidence is required.
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http://dx.doi.org/10.1042/BSR20211056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495431PMC
October 2021

Hand-related action words impair action anticipation in expert table tennis players: Behavioral and neural evidence.

Psychophysiology 2021 Sep 18:e13942. Epub 2021 Sep 18.

School of Psychology, Shanghai University of Sport, Shanghai, China.

Athletes extract kinematic information to anticipate action outcomes. Here, we examined the influence of linguistic information (experiment 1, 2) and its underlying neural correlates (experiment 2) on anticipatory judgment. Table tennis experts and novices remembered a hand- or leg-related verb or a spatial location while predicting the trajectory of a ball in a video occluded at the moment of the serve. Experiment 1 showed that predictions by experts were more accurate than novices, but experts' accuracy significantly decreased when hand-related words versus spatial locations were memorized. For nonoccluded videos with ball trajectories congruent or incongruent with server actions in experiment 2, remembering hand-related verbs shared cognitive resources with action anticipation only in experts, with heightened processing load (increased P3 amplitude) and more efficient conflict monitoring (decreased N2 amplitude) versus leg-related verbs. Thus, action anticipation required updating of motor representations facilitated by motor expertize but was also affected by effector-specific semantic representations of actions, suggesting a link from language to motor systems.
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http://dx.doi.org/10.1111/psyp.13942DOI Listing
September 2021

Pan-Cancer Analysis of PARP1 Alterations as Biomarkers in the Prediction of Immunotherapeutic Effects and the Association of Its Expression Levels and Immunotherapy Signatures.

Front Immunol 2021 31;12:721030. Epub 2021 Aug 31.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: Poly (ADP-ribose) polymerases-1 (PARP1) alterations are associated with PARP1 inhibitor resistance, regulating the function of Treg cells and PDL1 expression in tumor cells, and high PARP1 expression is significantly associated with aggressive behavior and chemotherapeutic resistance in several tumors. However, a comprehensive analysis of the predictive values of PARP1 alteration for immune checkpoint inhibitor (ICI) effectiveness in tumors remains unclear, and the associations between its expression and immunotherapy signatures also needs to be explored further.

Methods: We performed some analyses with the cBioPortal online database (https://www.cbioportal.org), TIMER2.0 (Tumor Immune Estimation Resource 2.0, http://timer.comp-genomics.org/) and TCGA database (https://xenabrowser.net or https://portal.gdc.cancer.gov/). Survival analysis was conducted using Kaplan-Meier method, and the associations between PARP1 transcription levels and immune checkpoint gene expression, the number of neoantigens, tumor mutation burden (TMB) levels, and microsatellite instability (MSI) event are analyzed by spearman correlation analysis and visualization of those mentioned above is performed using R, version 3.6.3 (http://www.r-project.org/).

Results: We found that PARP1 was altered in 1338 (2.9%) out of 45604 patients with diverse tumors, which was associated with markedly higher TMB levels in a variety of tumors (P < 0.01). Impressively, patients with PARP1 alterations in advanced tumors showed better overall survival (OS) in the ICI-treated cohort (P = 0.016). PARP1 altered group was substantially correlated with higher immune infiltrates across most tumors, including CD8+ T cells in colorectal adenocarcinoma (P = 0.0061), endometrial carcinoma (P = 0.0033), stomach cancer (P = 0.033), and cervical cancer (P = 0.026), respectively. The PARP1 altered group showed high expression in transcription (P < 0.001), and higher expression of LAG3, PDCD1, CTLA-4, and TIGIT (P < 0.05). Higher PARP1 expression was present in 27 tumor compared the corresponding normal tissues using the GTEx and TCGA databases and it had a worse OS in several tumors (P < 0.05). Further, high PARP1 expression was significantly associated with six immune cells (B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells) in most tumors, including colon adenocarcinoma (COAD), head and neck squamous cell carcinoma (HNSC), kidney renal clear cell carcinoma (KIRC), and liver hepatocellular carcinoma (LIHC) (P < 0.05). In particular, CD8+T cell infiltration, was also positively correlated with high PARP1 expression in bladder urothelial carcinoma (BLCA), breast invasive carcinoma (BRCA), kidney renal papillary cell carcinoma (KIRP), brain lower grade glioma (LGG), LIHC, pancreatic adenocarcinoma (PAAD), pheochromocytoma and paraganglioma (PCPG), prostate adenocarcinoma (PRAD), rectum adenocarcinoma (READ), testicular germ cell tumors (TGCT), thymoma (THYM), uterine corpus endometrial carcinoma (UCEC), uveal melanoma (UVM) (P < 0.05, no data shown), and PARP1 expression was significantly positively correlated with the transcription levels of some of the 47 immune checkpoint genes, such as CD274, CTLA4, and PDCD1 in several tumors, including PAAD, LIHC, KIRC, HNSC, and BLCA (P < 0.05). A significant positive association between PARP1 expression and the number of immune neoantigen was found within COAD, KIRC, lung adenocarcinoma (LUAD), PAAD and THYM (P < 0.05), and there were also significantly positive correlations between PARP1 expression and TMB in many tumors like adrenocortical carcinoma (ACC), COAD, kidney chromophobe (KICH), LGG, LUAD, READ, skin cutaneous melanoma (SKCM) and stomach adenocarcinoma (STAD) (P < 0.05). In addition, high PARP1 expression was positively associated with microsatellite instability event in COAD, KIRP, BRCA, glioblastoma multiforme (GBM), lung squamous cell carcinoma (LUSC), LGG, READ, UCEC, SKCM and LUAD (P < 0.05).

Conclusions: Our results highlight the significance of PARP1 alterations as pan-cancer predictive biomarkers for ICI treatment, and its expression levels seem to be correlated with the status of immunotherapy-associated signatures, thus may be a promising biomarker for predicting ICI response in several tumors.
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http://dx.doi.org/10.3389/fimmu.2021.721030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438309PMC
August 2021

Study of the Correlation Between the Ratio of Diastolic to Systolic Durations and Echocardiography Measurements and Its Application to the Classification of Heart Failure Phenotypes.

Int J Gen Med 2021 10;14:5493-5503. Epub 2021 Sep 10.

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Background: This study aimed to investigate the correlation between the ratio of diastolic to systolic durations (D/S) and echocardiographic parameters of patients with chronic heart failure (CHF) and evaluate whether the D/S can be used as a supplementary biomarker for the classification of heart failure (HF) phenotypes.

Methods: In total, 122 CHF patients with a left ventricular ejection fraction (LVEF) <40%, 40%≤LVEF<50%, or ≥50% were categorized as having HF with a reduced ejection fraction (HFrEF) (N=32), HF with a mid-range ejection fraction (HFmrEF) (N=21) or HF with a preserved ejection fraction (HFpEF) (N=69), respectively. All patients underwent echocardiography for assessment of nineteen structural and functional echocardiographic parameters and digital phonocardiography for the measurement of D/S. Spearman correlation was used to analyse the associations between the D/S and echocardiographic parameters. Multivariate logistic regression analysis was performed to examine the associations between the D/S and HF phenotypes, and receiver operating characteristic (ROC) curve analysis was employed to evaluate the predictive value of the D/S in the classification of HF phenotypes.

Results: The D/S values of patients with HFrEF, HFmrEF and HFpEF were 1.32±0.06, 1.44±0.11 and 1.54±0.08, respectively, which were significantly different (All P<0.05). A close correlation between the D/S and LVEF was found (r=0.777, P<0.001). The multivariate analysis indicated that the D/S was an independent risk factor for CHF phenotypes (OR=4.927, 95% CI 2.532-9.587; P<0.001). The area under the ROC curve for distinguishing between HFmrEF and HFpEF using the D/S was 0.764 (95% CI 0.707-0.845; P < 0.001) and that for distinguishing between HFmrEF and HFrEF using the D/S was 0.821 (95% CI 0.755-0.882; P < 0.001).

Conclusion: The D/S was significantly associated with LVEF, and as LVEF decreased, the D/S tended to decrease, which could also serve as a noninvasive supplementary indicator for detecting systolic and diastolic dysfunction.
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http://dx.doi.org/10.2147/IJGM.S324319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439664PMC
September 2021

Next-generation sequencing redefines the diagnosis of triple-negative myeloproliferative neoplasms.

Ann Hematol 2021 Sep 13. Epub 2021 Sep 13.

Department of Hematology, Zhongnan Hospital of Wuhan University, Donghu Road, No. 169, Wuhan, China.

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http://dx.doi.org/10.1007/s00277-021-04561-5DOI Listing
September 2021

Mechanism by Which Tong Xie Yao Fang Heals the Intestinal Mucosa of Rats with Ulcerative Colitis through the Hippo Pathway.

Evid Based Complement Alternat Med 2021 30;2021:5533914. Epub 2021 Aug 30.

Department of Anorectal, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China.

Objective: Tong Xie Yao Fang (TXYF) is a classic and effective prescription in traditional Chinese medicine which is used to treat ulcerative colitis (UC). Our study investigated the effect of TXYF on Hippo pathway activation in UC-induced intestinal mucosa injury and explored the possible mechanism.

Method: After ulcerative colitis was successfully induced by trinitrobenzene sulfonic acid (TNBS), 48 Sprague Dawley (SD) rats were randomly divided into a control group, model group, TXYF group, and sulfasalazine group and treated with the corresponding drugs for 28 days. The parameters including body weight, colon length, spleen index, and disease activity index (DAI) and histopathological characteristics were assessed. The myeloperoxidase (MPO) activity and IL-6 level in the colon mucosa were determined with the corresponding commercial kits. The expressions of the Hippo pathway components YAP1, TAZ, P-YAP, and LATS1 were detected in the colon mucosa of each group on different stages by quantitative real-time PCR (qRT-PCR) and western blotting. Immunohistochemical staining was used to evaluate the growth and apoptosis of the colon epithelium.

Result: TXYF significantly improved the weight loss, colonic shortening, DAI, spleen enlargement, and histopathological score of the rats with TNBS-induced UC. TXYF also reduced the MPO activity and expression of IL-6 in the colon mucosa. Furthermore, treatment with TXYF significantly increased YAP1 expression in the early stage (3-7 days) and significantly decreased YAP1 expression in the late stage (14-28 days). In the early stage, TXYF inhibited Hippo pathway activity, which promoted proliferation and regeneration of the intestinal mucosa. In the late stage, the Hippo pathway was activated, thereby inhibiting apoptosis and promoting intestinal mucosal differentiation.

Conclusion: TXYF alleviated the inflammatory response and promoted mucosal healing in rats with UC, which was probably achieved through the Hippo pathway. These results indicated that TXYF was a potential therapy for treating UC.
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http://dx.doi.org/10.1155/2021/5533914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423548PMC
August 2021

Pilot-scale bioaugmentation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil using an indigenous bacterial consortium in soil-slurry bioreactors.

Chemosphere 2021 Sep 4;287(Pt 2):132183. Epub 2021 Sep 4.

Engineering Research Center of Ministry of Education on Groundwater Pollution Control and Remediation, College of Water Sciences, Beijing Normal University, Beijing, 100875, PR China. Electronic address:

Soil-slurry bioreactor based bioremediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil was studied through laboratory and pilot-scale trials, in which the degradation mechanism was explored. Indigenous PAH-degrading consortium was firstly screened out and it degraded 80.5% of total PAHs in lab-scale bioreactors. Then a pilot-scale trial lasting 410 days was conducted in two bioreactors of 1.5 m to examine the operating parameters and validate the optimum running conditions. During the initial 200 days, the crucial running parameters affecting PAH removal were evaluated and selected. Subsequently, an average PAH removal rate of 93.4% was achieved during 15 consecutive batches (210 days) under the optimum running conditions. The kinetic analysis showed that the reactor under optimum conditions achieved the highest PAH degradation rate of 0.1795 day and the shortest half-life of 3.86 days. Notably, efficient mass transfer of PAHs and high biodegradation capability by bioaugmented consortia in soil-slurry bioreactors were two key mechanisms for appreciable PAH removal performance. Under the optimal operating conditions, the degradation rate of low-molecular-weight (LMW) PAHs was significantly higher than high-molecular-weight (HMW) PAHs; when the mass transfer was limited, there was no significant difference between their degradation behaviors. Both microbial co-metabolism and collaborative metabolism might occur when all PAHs demonstrated low degradation rates. The findings provide insightful guidance on the future assessment and remediation practices of PAH-contaminated sites.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132183DOI Listing
September 2021

Increased Risk of Vitamin D Deficiency Among HIV-Infected Individuals: A Systematic Review and Meta-Analysis.

Front Nutr 2021 18;8:722032. Epub 2021 Aug 18.

Center for Infectious Diseases, Beijing Youan Hospital, Capital Medical University, Beijing, China.

Human immunodeficiency virus (HIV) infection is a heavy burden worldwide. Observational studies have reported a high prevalence of vitamin D deficiency (VDD) among people living with HIV (PLWH). However, its deficiency is also a global health problem. Therefore, we conducted a meta-analysis and systemic review to compare differences between HIV-infected subjects and non-HIV-infected subjects. We searched PubMed, Web of Science, Embase, and Cochrane library. We extracted data, including demographic information, study type, vitamin D-related values, and HIV-related values, ultimately including 15 studies after removing duplicates and screening titles, abstracts, and full texts and finally performing a meta-analysis in terms of vitamin D level and vitamin D deficiency prevalence. Regarding VDD prevalence, the HIV vs. the non-HIV group had an odds ratio of 1.502 (95% CI, 1.023-2.205; = 0.038). In the subgroup analysis, the odds ratios were 1.647 (95% CI, 1.020-2.659; = 0.041; = 94.568) from 7 studies (age over 40), 2.120 (95% CI, 1.122-4.008; = 0.021; = 0.000) from 2 studies (BMI less than or equal to 25), 1.805 (95% CI, 1.373-2.372; = 0.042; = 74.576) from 7 studies (latitude <40), 2.120 (95% CI, 1.122-4.088; = 0.021; = 0.000) from 2 studies (only included male participants), and 2.296 (95% CI, 1.287-4.097; = 0.005; = 19.927) from 3 studies (only included ART-experienced participants). Thirteen studies were deemed to have moderate quality, while two had high quality. HIV infected subjects are prone to have VDD compared with general population. ART, older age, lower BMI, lower latitude and male sex may present risk factors for VDD in PLWH. https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=228096.
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http://dx.doi.org/10.3389/fnut.2021.722032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418196PMC
August 2021

Association study of relationships of polymorphisms in the miR-21, miR-26b, miR-221/222 and miR-126 genes with cervical intraepithelial neoplasia and cervical cancer.

BMC Cancer 2021 Sep 7;21(1):997. Epub 2021 Sep 7.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, Yunnan, China.

Background: miR-21, miR-26b, miR-221/222 and miR-126 play crucial roles in cervical cancer development. Studies have shown that polymorphisms in miRNA genes can affect miRNA expression, which might be associated with cancer development.

Methods: Ten single-nucleotide polymorphisms (SNPs) in the miR-21, miR-26b, miR-221/222 and miR-126 genes (rs1292037, rs13137 in miR-21; rs2227255, rs2227258 in miR-26b; rs2858061, rs34678647, rs2858060, rs2745709 in miR-221/222; rs2297537, rs2297538 in miR-126) were selected, and genotyped in a total of 2176 individuals, including 435 patients with cervical intraepithelial neoplasia (CIN), 743 patients with cervical cancer (CC) and 998 healthy persons using TaqMan assays, and their associations with CIN and CC were evaluated.

Results: Our results showed significant differences for the rs2297538 genotypes between the CIN and CC groups (P = 0.001). In addition, our results also showed significant differences for the rs2297537 alleles between the CIN and CC groups (P = 0.003), and the C allele of rs2297537 might be associated with a decreased risk of CC (OR = 0.72, 95%CI: 0.58-0.90). At the inheritance analysis, between the CIN and control groups, the T/T-T/C genotype in rs1292037 and A/A-A/T genotype in rs13137 might be associated with an increased risk of CIN in the recessive model (OR = 1.61, 95% CI: 1.17-2.20 and OR = 1.58, 95% CI: 1.15-2.15). In addition, the C/C-T/T genotype of rs2745709 might be associated with a decreased risk of CIN in the overdominant model (OR = 0.66, 95% CI: 0.52-0.82). Between, CIN and CC group, the T/T-C/C genotype in rs1292037 and A/A-T/T genotype in rs13137 might be associated with an increased risk of CC in the overdominant model (OR = 1.43, 95% CI: 1.12-1.81 and OR = 1.42, 95% CI: 1.12-1.80). The rs2297538 G/G-A/G genotype might be associated with an increased risk of CC in the recessive model (OR = 2.83, 95% CI: 1.52-5.25). The rs2297537 2C/C + C/G genotype might be associated with a decreased risk of CC (OR = 0.71, 95% CI: 0.57-0.89) in the log-additive model. The rs2745709 T/T-C/C genotype might be associated with an increased risk of CC (OR = 1.44, 95% CI: 1.13-1.83) in the overdominant model.

Conclusion: Our results indicate that rs2297538 and rs2297537 in miR-126, rs1292037 and rs13137 in miR-21, and rs2745709 in miR-221/222, may have important roles in the development of CIN or CC.
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http://dx.doi.org/10.1186/s12885-021-08743-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422721PMC
September 2021

Synthetic exploration of sulfinyl radicals using sulfinyl sulfones.

Nat Commun 2021 09 2;12(1):5244. Epub 2021 Sep 2.

Department of Chemistry, Northeast Normal University, Changchun, China.

Sulfinyl radicals - one of the fundamental classes of S-centered radicals - have eluded synthetic application in organic chemistry for over 60 years, despite their potential to assemble valuable sulfoxide compounds. Here we report the successful generation and use of sulfinyl radicals in a dual radical addition/radical coupling with unsaturated hydrocarbons, where readily-accessed sulfinyl sulfones serve as the sulfinyl radical precursor. The strategy provides an entry to a variety of previously inaccessible linear and cyclic disulfurized adducts in a single step, and demonstrates tolerance to an extensive range of hydrocarbons and functional groups. Experimental and theoretical mechanistic investigations suggest that these reactions proceed through sequential sulfonyl and sulfinyl radical addition.
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http://dx.doi.org/10.1038/s41467-021-25593-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413321PMC
September 2021

Association Between Sleep Quality and Pain Intensity in Mild Patients with COPD: A Community Study.

J Pain Res 2021 25;14:2641-2649. Epub 2021 Aug 25.

Pudong New Area Center for Disease Control and Prevention, Pudong Preventive Medicine Research Institute of Fudan University, Shanghai, 200136, People's Republic of China.

Purpose: Poor sleep quality and pain were common and had been proved as an important influenced factor of quality of life for patients with COPD. The association of sleep quality with pain has been observed in other population but remains unclear in mild patients with COPD from a community setting.

Methods: A cross-sectional study was conducted to include eligible mild patients with COPD in Pudong New District of Shanghai. A structured questionnaire was used to collect general and clinical information for the patients. The Chinese version of Pittsburgh Sleep Quality Index (PSQI) and the short form of McGill Pain Questionnaire (SF-MPQ) was used to assess sleep quality and intensity of pain. Logistic regression was performed to test the association between sleeping quality and pain intensity.

Results: Two hundred and sixty-four patients with COPD, with an average age of 64 years (SD 5.78 years), were enrolled, and of 52% were women. Seventy-one (26.9%) participants reported at least one exacerbation during the past year. About 28.2% of the patients were classified as having poor sleep quality. Sleep quality was significantly associated with PRI score (adjusted odds ratio (ORad)=2.16, 95% CI: 1.16-4.00) and PPI rank (ORad=1.90, 95% CI: 1.08-3.34). People with daytime disturbance were more likely to have pain (ORad =2.03, 95% CI: 1.18-3.50).

Conclusion: Poor sleep quality was common in mild patients with COPD in community and was associated with higher pain intensity. Pain may involve an impairment of sleep quality.
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http://dx.doi.org/10.2147/JPR.S310036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403565PMC
August 2021

Changes in Sensorimotor Cortical Activation in Children Using Prostheses and Prosthetic Simulators.

Brain Sci 2021 Jul 27;11(8). Epub 2021 Jul 27.

Department of Biomechanics, University of Nebraska-Omaha, Omaha, NE 68182, USA.

This study aimed to examine the neural responses of children using prostheses and prosthetic simulators to better elucidate the emulation abilities of the simulators. We utilized functional near-infrared spectroscopy (fNIRS) to evaluate the neural response in five children with a congenital upper limb reduction (ULR) using a body-powered prosthesis to complete a 60 s gross motor dexterity task. The ULR group was matched with five typically developing children (TD) using their non-preferred hand and a prosthetic simulator on the same hand. The ULR group had lower activation within the primary motor cortex (M1) and supplementary motor area (SMA) compared to the TD group, but nonsignificant differences in the primary somatosensory area (S1). Compared to using their non-preferred hand, the TD group exhibited significantly higher action in S1 when using the simulator, but nonsignificant differences in M1 and SMA. The non-significant differences in S1 activation between groups and the increased activation evoked by the simulator's use may suggest rapid changes in feedback prioritization during tool use. We suggest that prosthetic simulators may elicit increased reliance on proprioceptive and tactile feedback during motor tasks. This knowledge may help to develop future prosthesis rehabilitative training or the improvement of tool-based skills.
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http://dx.doi.org/10.3390/brainsci11080991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392534PMC
July 2021

Effects of Konjac glucomannan with different viscosities on the rheological and microstructural properties of dough and the performance of steamed bread.

Food Chem 2022 Jan 13;368:130853. Epub 2021 Aug 13.

College of Food and Bioengineering, Henan University of Science and Technology, 471023 Luoyang, Henan Province, PR China.

Konjac glucomannan (KGM) is used as an additive to improve the properties of wheat products. The effects of three types of KGM on the rheological properties and microstructure of dough, as well as the performance of steamed bread were investigated in this study. Particularly, dough with KGM displayed new features such as reduced peak viscosity, breakdown and setback. As the viscosity of KGM increased, the stability of the dough structure increased, while the viscosity and fluidity of the dough decreased. More interestingly, the gluten film of dough also increased with increasing substitution level and viscosity of KGM. Consistently, KGM with higher viscosity improved the quality of steamed bread. Generally, three types of KGM have different effects on the rheological characteristics and microstructure of dough, as well as the performance of steamed bread, which provide useful information for the proper application of KGM in wheat-based foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.130853DOI Listing
January 2022

Electrochemical aptasensor based on gold modified thiol graphene as sensing platform and gold-palladium modified zirconium metal-organic frameworks nanozyme as signal enhancer for ultrasensitive detection of mercury ions.

J Colloid Interface Sci 2021 Aug 11;606(Pt 1):510-517. Epub 2021 Aug 11.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.

Gold modified thiol graphene ([email protected]) was prepared and applied as sensing platform for constructing the electrochemical aptasensor. While gold-palladium modified zirconium metal-organic frameworks ([email protected]) nanozyme was employed as signal enhancer for detecting mercury ions (Hg) sensitively. Herein, gold nanoparticles (Au NPs) were modified on HS-rGO to form the thin [email protected] layer. Then the substrate strand (Apt1) was modified on the platform through Au-S bond. The signal strand (Apt2) was further decorated on the platform in the presence of Hg. Herein, the Apt2 was labeled with [email protected] nanozyme, which exhibited catalase-like properties to catalyze HO, thereby generating the electrical signal. With the concentration of Hg increased, the amount of modified [email protected] increased, leading to the rise of current response. Since the current responses were linear with concentration of Hg, the detection of Hg can be achieved. Under the optimum conditions, the prepared electrochemical aptasensor exhibited wide linear range from 1.0 nmol/L to 1.0 mmol/L, along with a low detection limit of 0.16 nmol/L. Moreover, the electrochemical aptasensor showed excellent selectivity, reproducibility and stability, together with superior performance in actual water sample analysis. Therefore, this proposed electrochemical aptasensor may have promising applications and provide references for environmental monitoring and management.
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http://dx.doi.org/10.1016/j.jcis.2021.08.055DOI Listing
August 2021

Micro-and-nanometer topological gradient of block copolymer fibrous scaffolds towards region-specific cell regulation.

J Colloid Interface Sci 2021 Aug 8;606(Pt 1):248-260. Epub 2021 Aug 8.

South China Advanced Institute for Soft Matter Science and Technology, School of Molecular Science and Engineering, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology, Guangzhou 510640, China. Electronic address:

Regulating cell behavior and function by surface topography has drawn significant attention in tissue engineering. Herein, a gradient fibrous scaffold comprising anisotropic aligned fibers and isotropic annealed fibers was developed to provide a controllable direction of cell migration, adhesion, and spreading. The electrospun aligned fibers were engraved to create surface gradients with micro-and-nanometer roughness through block copolymer (BCP) self-assembly induced by selective solvent vapor annealing (SVA). The distinct manipulation of cell behavior by annealed fibrous scaffolds with tailored self-assembled nanostructure and welded fibrous microstructure has been illustrated by in situ/ex situ small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and in vitro cell culture. Further insights into the effect of integrated gradient fibrous scaffold were gained at the level of protein expression. From the perspective of gradient topology, this region-specific scaffold based on BCP fibers shows the prospect of guiding cell migration, adhesion and spreading and provides a generic method for designing biomaterials for tissue-engineering.
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http://dx.doi.org/10.1016/j.jcis.2021.08.021DOI Listing
August 2021

Loss of FAM60A attenuates cell proliferation in glioma via suppression of PI3K/Akt/mTOR signaling pathways.

Transl Oncol 2021 Nov 10;14(11):101196. Epub 2021 Aug 10.

Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wannan Medical College, Wuhu 241001, China; Central Laboratory, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, China; Non-coding RNA Research Center of Wannan Medical College, Wuhu 241001, China. Electronic address:

Background: Glioma is a common malignant tumor of the central nervous system with a high incidence and mortality. Family with sequence similarity 60 member A (FAM60A) is a new subunit of the Sin3 deacetylase complex. The clinical significance and biologic role of FAM60A in glioma remain unclear.

Methods: The expression of FAM60A in normal glial cells, glioma cells, and five-paired gliomas, and adjacent noncancerous tissues was quantified using real-time polymerase chain reaction (PCR) and western blotting. FAM60A protein expression in 179 archived, paraffin-embedded glioma samples was analyzed using immunohistochemistry. The roles of FAM60A in glioma cell proliferation and tumorigenicity were explored in vitro and in vivo. The underlying molecular mechanisms were elucidated using Western blot assay. Serum exosomal FAM60A levels of glioma patients were detected using electron microscopy, western blot, and real-time PCR.

Results: FAM60A expression was significantly up-regulated in glioma tissues and cell lines and positively associated with a worse outcome in glioma. Knockdown of FAM60A could inhibit glioma cell proliferation and tumorigenicity in vitro and in vivo. Besides, FAM60A expression was detectable in extracted serum exosomes with a higher expression in the glioma cancer group than in the normal group.

Conclusions: Loss of FAM60A attenuates cell proliferation in glioma by suppressing PI3K/Akt/mTOR signaling pathways. Therefore, FAM60A may act as a prognostic biomarker and therapeutic target for glioma.
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http://dx.doi.org/10.1016/j.tranon.2021.101196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363885PMC
November 2021

A prospective study of healthful and unhealthful plant-based diet and risk of overall and cause-specific mortality.

Eur J Nutr 2021 Aug 11. Epub 2021 Aug 11.

Department of Nutrition, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.

Purpose: Although emphasis has recently been placed on the importance of diet high in plant-based foods, the association between plant-based diet and long-term risk of overall and cause-specific mortality has been less studied. We aimed to investigate whether plant-based diet was associated with lower death risk.

Methods: This prospective cohort study used data from the US National Health and Nutrition Examination Survey. Diet was assessed using 24 h dietary recalls. We created three plant-based diet indices including an overall plant-based diet index (PDI), a healthful plant-based diet index (hPDI), and an unhealthful plant-based diet index (uPDI). Deaths from baseline until December 31, 2015, were identified. Multivariable-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated using Cox regression.

Results: We documented 4904 deaths among 40,074 participants after a median follow-up of 7.8 years. Greater adherence to PDI was associated with lower risk of overall (HR comparing extreme quintiles 0.80, 95% CI 0.73, 0.89, p < 0.001) and cancer-specific (HR = 0.68, 95% CI 0.55, 0.85, p < 0.001) mortality. These inverse associations remained for hPDI and overall mortality with a HR of 0.86 (95% CI 0.77, 0.95, p = 0.001), but not for cancer or CVD mortality. Conversely, uPDI was associated with higher risk of total (HR = 1.33, 95% CI 1.19, 1.48, p < 0.001) and CVD-specific (HR = 1.42, 95% CI 1.12, 1.79, p = 0.015) mortality.

Conclusions: Increased intake of a plant-based diet rich in healthier plant foods is associated with lower mortality risk, whereas a plant-based diet that emphasizes less-healthy plant foods is associated with high mortality risk among US adults.
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http://dx.doi.org/10.1007/s00394-021-02660-7DOI Listing
August 2021

Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5.

Mol Biol Rep 2021 Aug 9;48(8):5847-5855. Epub 2021 Aug 9.

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.

Background: The mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively.

Methods And Results: In the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR and western blot assay revealed that the expression and phosphorylation level of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl).

Conclusions: All data suggested that the MAPK cascades might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.
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http://dx.doi.org/10.1007/s11033-021-06570-zDOI Listing
August 2021

Limonoids with Diverse Oxidation Patterns of C-12 Indicating a Complete Ring C- Biogenetic Pathway from .

J Nat Prod 2021 08 6;84(8):2352-2365. Epub 2021 Aug 6.

Jiangsu Key Laboratory of Bioactive Natural Product Research, State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.

Munrolins A-Q (-), 17 new ring C- limonoids with diverse oxidative patterns of C-12, together with nine known analogues (-), were isolated from the CHCl extract of . The planar structures were elucidated by a combination of 1D and 2D NMR and HR-MS analyses, while the absolute configurations were confirmed by ECD calculations and single-crystal X-ray diffraction. Munrolins A () and B () were first identified as ring C- limonoids with a 12,13-ether bridge moiety. Munrolins C-J (-) have a rare reduced primary alcohol fragment, while munrolin Q () has an unusual ketal fragment formed by dehydration of C-12/14. These limonoids with diverse alcohol and aldehyde type C11/12 branches may be generated through different degrees of reduction after the Baeyer-Villiger oxidation at the C ring, as key intermediates to supplement the biogenetic pathway of ring C- limonoids. Compounds , , and could reverse the multidrug resistance of MCF-7/doxorubicin cells with reversal fold values of 5.2, 4.5, and 18.3, respectively.
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http://dx.doi.org/10.1021/acs.jnatprod.1c00519DOI Listing
August 2021

Factors of Negative Affect in Elderly Patients With Substance Use Disorders During COVID-19 Pandemic.

Front Psychiatry 2021 20;12:697472. Epub 2021 Jul 20.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

The outbreak of the novel coronavirus disease 2019 (COVID-19) has become the greatest public health emergency and has attracted global attention. During the COVID-19 pandemic, the negative affect (NA) of elderly patients with substance use disorders (SUDs) has also become a more serious public concern. The current study aims to clarify the NA and its influencing factors in elderly patients with SUDs during the pandemic. Two psychiatrists conducted semi-structured interviews with 77 SUD patients aged above 50 years to collect their demographical information and certain drug use characteristics. Barratt Impulse Scale and the Positive and Negative Affect Scale were used to obtain information about patients' self-reported impulsivity and NA. Univariate linear regression analysis showed that NA was positively correlated with the frequency of drug use, type of SUDs, cravings during COVID-19, and impulsivity. Multiple linear regression analysis showed that being female, higher frequency of drug use, stronger cravings, and greater impulsiveness jointly accounted for the variation of NA in elderly patients with SUDs. This study confirmed that, during the COVID-19 pandemic, gender, frequency of drug use, cravings, and impulsivity were associated with NA in elderly patients with SUDs. This study provided a theoretical basis for clinicians to reduce the patients' NA.
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http://dx.doi.org/10.3389/fpsyt.2021.697472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329338PMC
July 2021

Antibody Response of an H9 Subtype Avian Influenza Poultry Vaccine on Three Kinds of Wild Birds in Shanghai Zoo.

Avian Dis 2021 03;65(1):90-94

Shanghai Zoo, Changning District, Shanghai, China 200335,

A semiannual immunization with a commercial inactivated H9 subtype avian influenza virus (AIV) vaccine developed for poultry has been used to prevent and control the avian influenza (AI) infections among captured wild birds in Shanghai Zoo. However, the overall safety and effectiveness of the poultry vaccine for housed birds in the zoo remain unclear. To verify the safety and efficacy of the commercial inactivated H9 AI vaccine on zoo birds and to explore a more reasonable and effective immunization procedure, 48 zoo birds, including 11 Oriental white storks, 25 peafowl, and 12 silver pheasants, were administered the AI vaccine developed for poultry use. Then, the clinical signs of the immunized birds were observed for 2 weeks, and the antibodies against H9 AI were determined via the hemagglutination inhibition test. Results showed that no harmful effects related to the vaccination were observed, and the antibody titers of the Oriental white stork, peafowl, and silver pheasants were all higher than 7 log at 21 days, 30 days, 60 days, 120 days, and 180 days postimmunization. For further study, the H9 AIV titers of 11 peafowls and 6 Oriental storks, which were raised in the nursing ground, were continuously monitored for 15 months. All of their antibody titers were above the national standards of China (5 log ; GB/T18936-2003), even at 12 months and 15 months postimmunization. We concluded that the commercial inactivated H9 AI vaccine used at the present time in Shanghai Zoo can induce high and prolonged immune responses in vaccinated birds.
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http://dx.doi.org/10.1637/aviandiseases-D-20-00094DOI Listing
March 2021
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