Publications by authors named "Yingying Li"

524 Publications

Simultaneous screening and analysis of 155 veterinary drugs in livestock foods using ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry.

Food Chem 2022 Nov 20;393:133260. Epub 2022 May 20.

AB SCIEX, Analytical Instrument Trading Co, Beijing 100015, China.

Veterinary drugs are widely used to improve the health and growth of livestock. The supervision of these residues is necessary to ensure food safety. A high-throughput method based on Oasis PRiME HLB with solid phase extraction for simultaneous qualitative and quantitative analysis of 155 veterinary drugs in livestock foods was developed by the ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry (UHPLC-QTRAP-MS). The limits of detection and quantification ranged from 0.5 μg/kg to 5 μg/kg and 2 μg/kg to 20 μg/kg, respectively. For over 85% of the analytes, the recoveries were between 60% and 120%. The positive simulated samples perfectly matched with a purity fit value over 70% from the self-built library. The screening results of UHPLC-QTRAP-MS were almost consistent with UHPLC tandem quadrupole-exactive orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap/MS). The evaluated UHPLC-QTRAP-MS method was powerful and reliable for the screening and quantification of veterinary drugs in real samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133260DOI Listing
November 2022

Chinese herbal medicine for children with idiopathic short stature (ISS): A systematic review and meta-analysis.

PLoS One 2022 24;17(6):e0270511. Epub 2022 Jun 24.

Department of Pediatrics, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, China.

Background: Idiopathic short stature (ISS) describes a heterogeneous group of children of many unidentified causes of short stature presently without definitive therapy. Chinese herbal medicine (CHM) is an alternative and complementary treatment for children with ISS and has been widely used for ISS while the evidence of its effectiveness is controversial. We conducted this systematic review and meta-analysis in order to evaluate the efficacy of CHM for ISS.

Methods: PubMed, Embase, Web of science, Sino-Med, Cochrane, CNKI, VIP, and Wangfang Data were electronically searched to collect randomized controlled trials (RCTs) of CHM treatment of ISS from inception to May 2021. Two researchers independently scanned the literature and extracted information on general characteristics, including patient, study design, interventions, and side effects, assessing the CHM intervention's efficacy and the risk of bias. Height, bone age, growth velocity, and IGF-1 level are the main consequences. Height standard deviations score (HtSDS), change in HtSDS (ΔHtSDS), osteocalcin, the peak level of growth hormones (GHP), and predicted adult height (PAH) are the secondary outcomes. Meta-analysis was then performed by using RevMan 5.3 (Cochrane Collaboration).

Results: Seven articles (569 participants) were included. The Meta-analysis indicated that herbal medicine was associated with increased height (MD 2.16 points; 95%CI, 0.22 to 4.10; P = 0.03), growth velocity (MD 1.47 points; 95%CI, 0.28 to 2.67; P = 0.02), IGF-1 level (MD 28.13 points; 95%CI, 22.80 to 33.46; P<0.00001) and GHP (MD 3.29 points; 95%CI, 1.54 to 5.04; P = 0.0002).

Conclusion: According to current research, CHM appears to be useful for children with ISS. Due to the limited quality and number of studies included, more high-quality studies are needed to corroborate the above conclusions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270511PLOS
June 2022

Synthesis and Biological Evaluation of Novel Synthetic Indolone Derivatives as Anti-Tumor Agents Targeting p53-MDM2 and p53-MDMX.

Molecules 2022 Jun 9;27(12). Epub 2022 Jun 9.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China.

A series of novel indolone derivatives were synthesized and evaluated for their binding affinities toward MDM2 and MDMX. Some compounds showed potent MDM2 and moderate MDMX activities. Among them, compound A13 exhibited the most potent affinity toward MDM2 and MDMX, with a of 0.031 and 7.24 μM, respectively. A13 was also the most potent agent against HCT116, MCF7, and A549, with IC values of 6.17, 11.21, and 12.49 μM, respectively. Western blot analysis confirmed that A13 upregulated the expression of MDM2, MDMX, and p53 by Western blot analysis. These results indicate that A13 is a potent dual p53-MDM2 and p53-MDMX inhibitor and deserves further investigation.
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http://dx.doi.org/10.3390/molecules27123721DOI Listing
June 2022

Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent.

Life (Basel) 2022 Jun 7;12(6). Epub 2022 Jun 7.

College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.

Tomato damping-off and root rot are the two most common diseases of tomatoes at the seedling stage. At present, biological compound seed-coating agents are gradually replacing chemical agents in preventing and controlling plant diseases and insect pests, regulating plant growth, and ensuring crop yields. In this study, five biocontrol bacteria ( (Ba), (Bs wy-1), (WXCDD105), (WXCDD51), and (WZ-37)), with broad antibacterial spectra were mixed with auxiliary factors (inactive components of seed-coating agent) after fermentation to compound a seed-coating agent. In this study, the formula for a compound seed-coating agent was selected through orthogonal experiment. Gaseous silica was used as a thickener, and gum arabic and sodium dodecylbenzene sulfonate were used as a film-forming agent and dispersant, respectively. The mass of fumed silica, gum arabic, sodium dodecylbenzene sulfonate, and pearlescent powder was 1.3 g, 1 g, 0.05 g, and 0.5 g, respectively. Adding gibberellin can improve the ability of seed-coating agents to promote seed germination and plant growth. This showed high efficiency in preventing and controlling seedling diseases and promoting seedling growth. After 6 days of inoculation with , which caused tomato damping-off disease, the seedling mortality rate was 26.7% lower than that of the sterile water control, and 20% lower than that of carbendazim. After 21 days of inoculation with sp., which caused tomato root rot disease, the seedling mortality rate was 44.31% lower than that of the control, and 22.36% lower than that of carbendazim. The plant height, stem diameter, root length, fresh weight, and dry weight of tomato seeds treated with biological compound seed-coating agent were significantly higher than that of the control. We tested the shelf life of the biological compound seed-coating agent, and found that the effect of seed germination and radicle growth did not decrease. This research provides information on the production technology and application of biological seed-coating agents in tomato production.
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http://dx.doi.org/10.3390/life12060849DOI Listing
June 2022

Three-Dimensional Open Water Microchannel Transpiration Mimetics.

ACS Appl Mater Interfaces 2022 Jun 23. Epub 2022 Jun 23.

Chinese Academy of Sciences Key Laboratory of Bio-inspired Materials and Interface Sciences, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

The key problem that hinders the water transportation performance and application of microchannels is the annoying gaslock. Realizing liquid transport without the gaslock requires a specially designed pump and a channel system, as well as the reduction of gas concentration in liquids. In nature, to eat viscous nectar with high efficiency, hummingbirds use their open geometric tongue for nectar-sucking. Inspired by hummingbirds' tongue, we report a bionic open microchannel that discharges unwanted gas inside the microchannel from the opening without influencing its fluidic performance. The opening can also be used for extrusion of oil droplets in microchannels, indicating great potential applications in oil-water separation and chemical slow release, especially for bubble discharge in microchannels. Most significantly, a mimicked "leaf" with our bionic open microchannnels exhibits marvelous "transpiration" performance when irradiated by a laser. Our work provides a new strategy for the fabrication of open microchannels and sheds light on potential applications of multiphase phenomena in microchannels including oil-water separation, phase change heat and mass transfer, solar vapor generation, and precisely controllable drug delivery.
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http://dx.doi.org/10.1021/acsami.2c09165DOI Listing
June 2022

On the Value of the Chinese Pre-Qin Confucian Thought of "Harmony" for Modern Public Mental Health.

Front Psychol 2022 2;13:870828. Epub 2022 Jun 2.

Health and Bioethics Research Center, Wenzhou Medical University, Wenzhou, China.

The Chinese pre-Qin Confucianism puts forward the concept of "Harmony," covering "Balanced Harmony," "External Harmony," and "Internal Harmony," etc. "Balanced Harmony" refers to the harmonious state of balance at both ends. "External Harmony" indicates the harmonious relationship with others, with society and with nature. "Internal Harmony" reveals the harmonious state within oneself between body and mind. "Balanced Harmony" is the ideal pursuit of Harmony, "External Harmony" and "Internal Harmony" are the two basic contents of Harmony, the trinity of which constitute a systematic concept of valuing "Harmony." The golden mean is the fundamental criterion for achieving "Balanced Harmony," while "Benevolent people love others" and "Seeking from the heart" are the two basic requirements for achieving "External Harmony" and "Internal Harmony" specifically, and together they constitute a systematic methodology of valuing "Harmony." As one of the backbones of traditional Chinese culture, the Chinese pre-Qin Confucian thought of "Harmony" not only has had a far-reaching impact on the temperament, mental state, cognitive style, and moral character of the Chinese nation in history but also provides insights for public mental health in modern times globally, containing a great deal of useful information for mental health issues from which Western public mental health could profit.
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http://dx.doi.org/10.3389/fpsyg.2022.870828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203267PMC
June 2022

Peripheral nerve defects repaired with autogenous vein grafts filled with platelet-rich plasma and active nerve microtissues and evaluated by novel multimodal ultrasound techniques.

Biomater Res 2022 Jun 11;26(1):24. Epub 2022 Jun 11.

Departments of Ultrasound, The First Center of Chinese PLA General Hospital, Beijing, China.

Background: Developing biocompatible nerve conduits that accelerate peripheral nerve regeneration, lengthening and functional recovery remains a challenge. The combined application of nerve microtissues and platelet-rich plasma (PRP) provides abundant Schwann cells (SCs) and various natural growth factors and can compensate for the deficiency of SCs in the nerve bridge, as well as the limitations of applying a single type of growth factor. Multimodal ultrasound evaluation can provide additional information on the stiffness and microvascular flow perfusion of the tissue. This study was designed to investigate the effectiveness of a novel tissue-engineered nerve graft composed of an autogenous vein, nerve microtissues and PRP in reconstructing a 12-mm tibial nerve defect and to explore the value of multimodal ultrasound techniques in evaluating the prognosis of nerve repair.

Methods: In vitro, nerve microtissue activity was first investigated, and the effects on SC proliferation, migration, factor secretion, and axonal regeneration of dorsal root ganglia (DRG) were evaluated by coculture with nerve microtissues and PRP. In vivo, seventy-five rabbits were equally and randomly divided into Hollow, PRP, Micro-T (Microtissues), Micro-T + PRP and Autograft groups. By analysing the neurological function, electrophysiological recovery, and the comparative results of multimodal ultrasound and histological evaluation, we investigated the effect of these new nerve grafts in repairing tibial nerve defects.

Results: Our results showed that the combined application of nerve microtissues and PRP could significantly promote the proliferation, secretion and migration of SCs and the regeneration of axons in the early stage. The Micro-T + PRP group and Autograft groups exhibited the best nerve repair 12 weeks postoperatively. In addition, the changes in target tissue stiffness and microvascular perfusion on multimodal ultrasound (shear wave elastography; contrast-enhanced ultrasonography; Angio PlaneWave UltrasenSitive, AngioPLUS) were significantly correlated with the histological results, such as collagen area percentage and VEGF expression, respectively.

Conclusion: Our novel tissue-engineered nerve graft shows excellent efficacy in repairing 12-mm defects of the tibial nerve in rabbits. Moreover, multimodal ultrasound may provide a clinical reference for prognosis by quantitatively evaluating the stiffness and microvescular flow of nerve grafts and targeted muscles.
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http://dx.doi.org/10.1186/s40824-022-00264-8DOI Listing
June 2022

3,3',5-triiodo-l-thyronine inhibits drug-induced liver injury through activation of PPARα as revealed by network pharmacology and biological experimental verification.

Toxicol Appl Pharmacol 2022 Aug 2;448:116098. Epub 2022 Jun 2.

Key Laboratory of TCM-information Engineer of State Administration of TCM, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address:

Drug-induced liver injury (DILI) has increased in recent years, leading to acute liver failure. 3,3',5-triiodo-l-thyronine (T3) has been reported to exert a potent hepatoprotective effect. However, the mechanism and efficacy of T3 on DILI remain undocumented. In this study, an MTT assay was used to detect the effect of T3 on hepatotoxicity of acetaminophen (APAP) in L02 cells. Then, we screened key targets and related biological pathways by network pharmacology. Finally, enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to verify the mechanism and key targets of T3 on DILI. The results of the MTT assay showed that T3 significantly decreased hepatocellular injury induced by APAP. Network pharmacology and bioinformatics analysis showed that 118 intersection targets of T3 and DILI were identified and the mechanism of T3 on DILI was related to cell proliferation and oxidative stress. ELISA results showed that T3 may be an effective treatment for DILI as biomarkers of hepatocellular injury such as AST, ALP were decreased compared to APAP only treated cells, and the mechanism of T3 may be mediated in part through improving redox balance. The topological parameter screening results suggested 12 key targets of T3 for DILI. Among them, PPARα is associated with DILI, and activation of PPARα can reduce oxidative stress and cell necrosis. Therefore, PPARα was identified as a target for verification. qRT-PCR analysis demonstrated that T3 could reverse the down-regulation of PPARα induced by APAP exposure. Taken together, we demonstrated for the first time that T3 could activate PPARα, promote cell proliferation and reduce oxidative stress, and play a vital role in the treatment of DILI, which provides a reference for T3 as a candidate treatment for DILI.
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http://dx.doi.org/10.1016/j.taap.2022.116098DOI Listing
August 2022

Analysis of interactions of immune checkpoint inhibitors with antibiotics in cancer therapy.

Front Med 2022 Jun 1. Epub 2022 Jun 1.

Pharmacy Department, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

The discovery of immune checkpoint inhibitors, such as PD-1/PD-L1 and CTLA-4, has played an important role in the development of cancer immunotherapy. However, immune-related adverse events often occur because of the enhanced immune response enabled by these agents. Antibiotics are widely applied in clinical treatment, and they are inevitably used in combination with immune checkpoint inhibitors. Clinical practice has revealed that antibiotics can weaken the therapeutic response to immune checkpoint inhibitors. Studies have shown that the gut microbiota is essential for the interaction between immune checkpoint inhibitors and antibiotics, although the exact mechanisms remain unclear. This review focuses on the interactions between immune checkpoint inhibitors and antibiotics, with an in-depth discussion about the mechanisms and therapeutic potential of modulating gut microbiota, as well as other new combination strategies.
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http://dx.doi.org/10.1007/s11684-022-0927-0DOI Listing
June 2022

Physical optimization of cell proliferation and differentiation using spinner flask and microcarriers.

AMB Express 2022 May 31;12(1):63. Epub 2022 May 31.

Beijing Institute of Food Science, Beijing, 100068, China.

The traditional breeding industry has been increasingly saturated and caused environmental pollution, disease transmission, excessive resource use, and methane emission; however, it still cannot meet the needs of the growing population. To explore other alternatives, researchers focused on cell agriculture and cell-based meat, especially large-scale cell culture. As a prerequisite for production, large-scale culture technology has become an important bottleneck restricting cell-based meat industrialization. In this study, the single-factor variable method was adopted to examine the influence of Cytodex1 microcarrier pretreatment, spinner flask reaction vessel, cell culture medium, serum and cell incubation, and other influencing factors on large-scale cell cultures to identify the optimization parameters suitable for 3D culture environment. Collagen and 3D culture were also prospectively explored to promote myogenesis and cultivate tissue-like muscle fibers that contract spontaneously. This research lays a theoretical foundation and an exploratory practice for large-scale cell cultures and provides a study reference for the microenvironment of myoblast culture in vitro, a feasible direction for the cell therapy of muscular dystrophy, and prerequisites for the industrialized manufacturing of cell-based meat. Graphical summary: Research on large-scale myoblast culture using spinner flasks and microcarriers. For cell culture, the microcarriers were pretreated with UV and collagen. Cell seeding condition, spinner flask speed, resting time, and spinner flask culture microenvironment were then optimized. Finally, two culture systems were prepared: a culture system based on large-scale cell expansion and a culture system for myogenesis promotion and differentiation.
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http://dx.doi.org/10.1186/s13568-022-01397-8DOI Listing
May 2022

High Efficacy Combined Microneedles Array with Methotrexate Nanocrystals for Effective Anti-Rheumatoid Arthritis.

Int J Nanomedicine 2022 24;17:2397-2412. Epub 2022 May 24.

School of Pharmacy, Bengbu Medical College, Bengbu, Anhui Province, 233030, People's Republic of China.

Introduction: Methotrexate (MTX) is the first-line drug for the treatment of rheumatoid arthritis (RA) in several countries. However, MTX has an extremely low solubility in water, and the side effects caused by its delivery mode restrict its curative effect. In this study, we designed a dissolving microneedles array (DMNA) containing MTX nanocrystals (MTX-NCs) ([email protected]) to improve the treatment of RA. DMNA-based drug delivery combines the advantages of patient compliance with the use of transdermal drug delivery systems and high-efficiency injection administration; thus, it can mitigate the side effects that result from current administration routes. Carrier-free and surfactant-free MTX-NCs were prepared to overcome bioavailability limitations and poor drug loading problems.

Methods: The MTX-NCs prepared by reverse solvent precipitation method was encapsulated in the DMNA. The morphology, mechanical properties, safety, stability and in vivo dissolution were evaluated, and its pharmacodynamic characteristics were assessed in a rat model of RA.

Results: The particle size of the MTX-NCs was 148.1 ± 10.1 nm. The [email protected] were found to be rigid enough to penetrate the skin and deliver the drug successfully. The results indicated effective skin recovery after removal of the DMNA. It was found that the [email protected] significantly reduced foot swelling in the rats and regulated the balance in the levels of related cytokines. It also reduced pathological damage to the synovium, joint, and cartilage, and effectively alleviated organ injury in the rats.

Conclusion: Transdermal administration of [email protected] may be an effective approach for treating RA.
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http://dx.doi.org/10.2147/IJN.S365523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148202PMC
June 2022

Recombinant Expressing Grass Carp Reovirus VP6 Induces Mucosal Immunity Against Grass Carp Reovirus Infection.

Front Immunol 2022 11;13:914010. Epub 2022 May 11.

Key Laboratory of Fishery Drug Development of Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

Grass carp haemorrhagic disease caused by grass carp reovirus II is a serious disease of the aquaculture industry and vaccination is the only effective method of GCRV protection. In this study, was used as oral vaccine delivery to express the GCRV II VP6 protein. We evaluated the protective efficacy of the live vaccine strain to induce mucosal immune protection. After oral administration, the recombinant strains remained in the hindgut for antigen presentation and increased the survival rate 46.7% and the relative percent survival 42.9%, respectively versus control vaccination. Though alone can induce the inflammatory response by stimulating the mucosal immune system, the recombinant expressing VP6 greatly enhanced nonspecific immune responses expression of immune related genes of the fish. Furthermore, both systemic and mucosal immunity was elicited following oral immunization with the recombinant strain and this strain also elicited an inflammatory response and cellular immunity to enhance the protective effect. can therefore be utilized as a mucosal immune vector to trigger high levels of immune protection in fish at both the systemic and mucosal levels. is a promising candidate for oral vaccine delivery.
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http://dx.doi.org/10.3389/fimmu.2022.914010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132009PMC
June 2022

The R2R3 MYB Transcription Factor Regulates Abscisic Acid Response in .

Plants (Basel) 2022 May 21;11(10). Epub 2022 May 21.

Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China.

Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of , a R2R3 MYB transcription factor in regulating ABA responses in . RT-PCR results show that the expression level of was increased in response to ABA treatment. protoplasts transfection results show that was specifically localized in nucleus and it activated the reporter gene when recruited to the promoter by a fused DNA binding domain GD. Roles of in regulating plant response to ABA were analyzed by generating transgenic plants overexpression and gene edited mutants of . The results show that ABA sensitivity was increased in the transgenic plants overexpression , but decreased in the mutants. By using a DEX inducible system, we further identified genes are likely regulated by , and found that they are enriched in biological process to environmental stimuli including abiotic stresses, suggesting that may regulate plant response to abiotic stresses. Taken together, our results suggest that is an ABA responsive gene, and functions as a transcription activator and it positively regulates ABA response in .
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http://dx.doi.org/10.3390/plants11101369DOI Listing
May 2022

Xihuang Pill enhances anticancer effect of anlotinib by regulating gut microbiota composition and tumor angiogenesis pathway.

Biomed Pharmacother 2022 Jul 20;151:113081. Epub 2022 May 20.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China. Electronic address:

Lung cancer poses a serious threat to human health. Although targeted therapies have led to breakthroughs in the treatment of lung cancer, drug resistance and side effects limit their clinical applications. Xihuang pill (XHW), a classical anti-cancer traditional Chinese medicine formula, has been clinically proven to be an effective complementary therapy in the treatment of various of cancers. However, the underlying mechanism for its use in combination with anti-cancer drugs remains unclear. Here, we explored the anti-lung cancer effect of XHW combined with anlotinib in mice bearing Lewis lung cancer (LLC). We used gut microbiota and transcriptomics to elucidate the regulatory properties of XHW in improving anti-lung cancer effect of anlotinib. The results showed that combination treatment of XHW with Anlotinib significantly inhibited tumor growth in LLC-bearing mice. We found that XHW played a key role in the regulation of gut microbiota using 16 s rRNA sequencing analysis. Specifically, XHW increased the proportion of the beneficial bacteria Bacteroides and g_norank_f_Muribaculaceae. Based on transcriptomic analysis of tumor tissues, differentially expressed genes in the combination therapy group were related to biological processes concerning angiogenesis, such as regulation of blood vessel diameter, regulation of tube diameter, and regulation of tube size. Our data suggest that XWH enhances the anticancer effect of anlotinib by regulating gut microbiota composition and tumor angiogenesis pathway. Combination therapy with anlotinib and XHW may be a novel therapeutic strategy for lung cancer patients.
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http://dx.doi.org/10.1016/j.biopha.2022.113081DOI Listing
July 2022

Endogenous Hydrogen Sulfide Persulfidates Caspase-3 at Cysteine 163 to Inhibit Doxorubicin-Induced Cardiomyocyte Apoptosis.

Oxid Med Cell Longev 2022 4;2022:6153772. Epub 2022 May 4.

Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.

Doxorubicin (DOX) is an efficient antitumor anthracycline drug, but its cardiotoxicity adversely affects the prognosis of the patients. In this study, we explored whether endogenous gasotransmitter hydrogen sulfide (HS) could protect against DOX-induced cardiomyocyte apoptosis and its mechanisms. The results indicated that DOX significantly downregulated endogenous HS production and endogenous synthetase cystathionine -lyase (CSE) expression and obviously stimulated the apoptosis in H9C2 cells. The supplement of HS donor sodium hydrosulfide (NaHS) or overexpression of CSE inhibited DOX-induced H9C2 cell apoptosis. DOX enhanced the activities of caspase family members in cardiomyocytes, while NaHS attenuated DOX-enhanced caspase-3, caspase-2, and caspase-9 activities by 223.1%, 73.94%, and 52.29%, respectively. Therefore, taking caspase-3 as a main target, we demonstrated that NaHS or CSE overexpression alleviated the cleavage of caspase-3, suppressed caspase-3 activity, and inhibited the cleavage of poly ADP-ribose polymerase (PARP). Mechanistically, we found that HS persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished HS-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of HS on caspase-3 in H9C2 cells. The mutation of caspase-3 C148S and C170S failed to block caspase-3 persulfidation by HS in H9C2 cells. However, caspase-3 C163S mutation successfully abolished the effect of HS on caspase-3 persulfidation and the corresponding protection of H9C2 cells. Collectively, these findings indicate that endogenous HS persulfidates caspase-3 at cysteine 163, inhibiting its activity and cardiomyocyte apoptosis. Sufficient endogenous HS might be necessary for the protection against myocardial cell apoptosis induced by DOX. The results of the study might open new avenues with respect to the therapy of DOX-stimulated cardiomyopathy.
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http://dx.doi.org/10.1155/2022/6153772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095366PMC
May 2022

Knowledge, attitudes and willingness to organ donation among the general public: a cross-sectional survey in China.

BMC Public Health 2022 05 9;22(1):918. Epub 2022 May 9.

Centre of Health and Bioethics Research, Wenzhou Medical University, Wenzhou Chashan Higher Education Park, Wenzhou, 325035, Zhegjiang, China.

Background: The purpose of this study is to assess the level of knowledge, attitudes, and willingness to organ donation among the general public in China.

Methods: The study population consisted of 4274 participants from Eastern, Central and Western China. The participants' knowledge, attitudes and willingness to organ donation were collected by a self-designed questionnaire consisting of 30 items. Knowledge is measured by 10 items and presented as a 10 point score, attitudes is measured by 20 items using a 5-step Likert scale and total score ranged between 0 and 80; while the willingness to donate is assessed as binary variable (0 = No; 1 = Yes). A logistic regression model was used to assess the association of knowledge and attitudes with willingness to organ donation, controlling for demographic and socioeconomic confounders.

Results: The questionnaire response rate was 94.98%. The mean score (± SD) of the general public's knowledge to organ donation was 6.84 ± 1.76, and the mean score (± SD) of attitudes to organ donation was 47.01 ± 9.07. The general public's knowledge and attitudes were the highest in Eastern China, followed by West and Central China. The logistic regression model indicated a positive association between knowledge and the willingness to organ donation (OR = 1.12, 95%CI: 1.08, 1.17; P < 0.001); attitudes were also positively potential determinant of more willingness to organ donation (OR = 1.08, 95%CI: 1.07, 1.09; P < 0.001).

Conclusions: Knowledge and attitudes were found to be positively associated with the Chinese general public's willingness to organ donation. Knowledge about the concept of brain death and the transplant procedure may help raise the rate of willingness to organ donation.
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http://dx.doi.org/10.1186/s12889-022-13173-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082919PMC
May 2022

Carbohydrate-to-protein ratio regulates hydrolysis and acidogenesis processes during volatile fatty acids production.

Bioresour Technol 2022 Jul 5;355:127266. Epub 2022 May 5.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.

In this study, the typical model solubilized and hydrolyzed substrates of protein and carbohydrate were anaerobically fermented at different carbohydrate-to-protein (Car/Pro) ratios to examine volatile fatty acids (VFAs) production and substrate consumption. The highest VFAs yields of 0.71 and 0.72 mg COD/mg COD both occurred at Car/Pro ratio of 1 by BSA-dextran and amino acids (AAs)-glucose fermentation, respectively. The limiting processes were hydrolysis and acidogenesis for the higher Car/Pro ratio of 3 and lower Car/Pro ratio of 0.25, respectively. An inhibitory effect of AAs accumulation was found, and VFAs production could be raised by 25.5% through quartic fed-batch strategy. There existed a significant relationship between activity of hydrolytic and acid-forming enzymes and acidogenic metabolism efficiency, which could be fitted by first-order kinetic and Logistic-based models. Understanding the effects of Car/Pro ratio on VFAs production is of guiding significance for regulating hydrolysis and acidogenesis processes during anaerobic fermentation of organic wastes.
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http://dx.doi.org/10.1016/j.biortech.2022.127266DOI Listing
July 2022

Application of high precision wavelength calibration method for plasmas rotation measurement based on Fabry-Pérot etalon on experimental advanced superconducting tokamak.

Rev Sci Instrum 2022 Apr;93(4):043504

School of Nuclear Science and Technology, University of South China, Hengyang, Hunan 421001, China.

Analyzing the radiation spectra of impurity ions is a widely applied diagnostic scheme for plasma ion temperature and rotation measurements on tokamaks. High precision wavelength calibration is a prerequisite for the accurate measurement of plasma parameters, especially for plasma rotation. Furthermore, the sparseness or absence of the standard spectral lines brings calibration challenges due to the narrow wavelength range. A precise wavelength calibration method is demonstrated in which the comb-like spectra generated by the Fabry-Pérot etalon can lock a series of fixed peaks as reference points in a wide wavelength range. The equal frequency intervals of the comb-like spectra are further corrected using several characteristic neon lines of known wavelengths. The experimental results indicate that the wavelength accuracy obtained by this calibration method is less than 0.005 nm, which corresponds to a rotation speed of 2.3 km/s in the toroidal direction for the beam emission spectroscopy spectrometer installed on the experimental advanced superconducting tokamak. Taking the O V(650.024 nm, n = 4 → 3) line as an example, the maximum difference in the oxygen ion rotation velocity is 3.8 km/s for the absolute rotation of ∼25 km/s, when compared with the calibration results of a standard lamp.
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http://dx.doi.org/10.1063/5.0083784DOI Listing
April 2022

Activated Ni-OH Bonds in a Catalyst Facilitates the Nucleophile Oxidation Reaction.

Adv Mater 2022 Apr 26:e2105320. Epub 2022 Apr 26.

State Key Laboratory of Chem/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, P. R. China.

The nucleophile oxidation reaction (NOR) is of enormous significance for organic electrosynthesis and coupling for hydrogen generation. However, the nonuniform NOR mechanism limits its development. For the NOR, involving electrocatalysis and organic chemistry, both the electrochemical step and non-electrochemical process should be taken into account. The NOR of nickel-based hydroxides includes the electrogenerated dehydrogenation of the Ni -OH bond and a spontaneous non-electrochemical process; the former determines the electrochemical activity, and the nucleophile oxidation pathway depends on the latter. Herein, the space-confinement-induced synthesis of Ni Fe layered double hydroxide intercalated with single-atom-layer Pt nanosheets (Ni Fe LDH-Pt NS) is reported. The synergy of interlayer Pt nanosheets and multiple defects activates Ni-OH bonds, thus exhibiting an excellent NOR performance. The spontaneous non-electrochemical steps of the NOR are revealed, such as proton-coupled electron transfer (PCET; Ni -O + X-H = Ni -OH + X ), hydration, and rearrangement. Hence, the reaction pathway of the NOR is deciphered, which not only helps to perfect the NOR mechanism, but also provides inspiration for organic electrosynthesis.
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http://dx.doi.org/10.1002/adma.202105320DOI Listing
April 2022

Identification of Gene Family in and Its Function Analysis under Stress.

Int J Mol Sci 2022 Apr 11;23(8). Epub 2022 Apr 11.

College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.

Tomato is an important vegetable crop. In the process of tomato production, it will encounter abiotic stress, such as low temperature, drought, and high salt, and biotic stress, such as pathogen infection, which will seriously affect the yield of tomato. Calcium-dependent protein kinase (CDPK) is a class of major calcium signal receptor which has an important regulatory effect on the perception and decoding of calcium signals. CDPK plays a key role in many aspects of plant growth, such as the elongation of pollen tubes, plant growth, and response to biotic and abiotic stress. While some studies have concentrated on and pepper, is a wild species relative of cultivated tomato and there is no report on CDPK in to date. Using tomato genomic data, this study identified 33 members of the gene family. Evolutionary analysis divides family members into four Asian groups, of which the CDPK family members have 11 gene replication pairs. Subcellular location analysis showed that most proteins were predicted to be located in the cytoplasm, and less protein existed on the cell membrane. Not all CDPK family members have a transmembrane domain. Cis regulatory elements relating to light, hormones, and drought stress are overrepresented in the promoter region of the genes in . The expression levels of each gene under biotic stress and abiotic stress were quantified by qRT-PCR. The results showed that members of the CDPK family in respond to different biotic and abiotic stresses. Among them, the expression of and genes change significantly. and genes were silenced using VIGS (virus-induced gene silencing), and the silenced plants illustrated reduced stress resistance to cold, and drought stress. The results of this study will provide a basis for the in-depth study of the gene family in , laying the foundation for further analysis of the function of the gene family.
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http://dx.doi.org/10.3390/ijms23084227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031491PMC
April 2022

Bright, Magnetic NIR-II Quantum Dot Probe for Sensitive Dual-Modality Imaging and Intensive Combination Therapy of Cancer.

ACS Nano 2022 Apr 20. Epub 2022 Apr 20.

CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.

Improving the effectiveness of cancer therapy will require tools that enable more specific cancer targeting and improved tumor visualization. Theranostics have the potential for improving cancer care because of their ability to serve as both diagnostics and therapeutics; however, their diagnostic potential is often limited by tissue-associated light absorption and scattering. Herein, we develop [email protected]:Mn quantum dots (QDs) with intrinsic multifunctionality that both enable the accurate localization of small metastases and act as potent tumor ablation agents. By leveraging the growth kinetics of a ZnS shell on a biocompatible CuInSe core, Mn doping, and folic acid functionalization, we produce biocompatible QDs with high near-infrared (NIR)-II fluorescence efficiency up to 31.2%, high contrast on magnetic resonance imaging (MRI), and preferential distribution in 4T1 breast cancer tumors. MRI-enabled contrast of these nanoprobes is sufficient to timely identify small metastases in the lungs, which is critically important for preventing cancer spreading and recurrence. Further, exciting tumor-resident QDs with NIR light produces both fluorescence for tumor visualization through radiative recombination pathways as well as heat and radicals through nonradiative recombination pathways that kill cancer cells and initiate an anticancer immune response, which eliminates tumor and prevents tumor regrowth in 80% of mice.
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http://dx.doi.org/10.1021/acsnano.2c01153DOI Listing
April 2022

Tea plant roots respond to aluminum-induced mineral nutrient imbalances by transcriptional regulation of multiple cation and anion transporters.

BMC Plant Biol 2022 Apr 19;22(1):203. Epub 2022 Apr 19.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China.

Background: Tea is one of the most popular non-alcoholic beverages in the world for its flavors and numerous health benefits. The tea tree (Camellia sinensis L.) is a well-known aluminum (Al) hyperaccumulator. However, it is not fully understood how tea plants have adapted to tolerate high concentrations of Al, which causes an imbalance of mineral nutrition in the roots.

Results: Here, we combined ionomic and transcriptomic profiling alongside biochemical characterization, to probe the changes of metal nutrients and Al responsive genes in tea roots grown under increasing concentrations of Al. It was found that a low level of Al (~ 0.4 mM) maintains proper nutrient balance, whereas a higher Al concentration (2.5 mM) compromised tea plants by altering micro- and macro-nutrient accumulation into roots, including a decrease in calcium (Ca), manganese (Mn), and magnesium (Mg) and an increase in iron (Fe), which corresponded with oxidative stress, cellular damage, and retarded root growth. Transcriptome analysis revealed more than 1000 transporter genes that were significantly changed in expression upon Al exposure compared to control (no Al) treatments. These included transporters related to Ca and Fe uptake and translocation, while genes required for N, P, and S nutrition in roots did not significantly alter. Transporters related to organic acid secretion, together with other putative Al-tolerance genes also significantly changed in response to Al. Two of these transporters, CsALMT1 and CsALS8, were functionally tested by yeast heterologous expression and confirmed to provide Al tolerance.

Conclusion: This study shows that tea plant roots respond to high Al-induced mineral nutrient imbalances by transcriptional regulation of both cation and anion transporters, and therefore provides new insights into Al tolerance mechanism of tea plants. The altered transporter gene expression profiles partly explain the imbalanced metal ion accumulation that occurred in the Al-stressed roots, while increases to organic acid and Al tolerance gene expression partly explains the ability of tea plants to be able to grow in high Al containing soils. The improved transcriptomic understanding of Al exposure gained here has highlighted potential gene targets for breeding or genetic engineering approaches to develop safer tea products.
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http://dx.doi.org/10.1186/s12870-022-03570-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017051PMC
April 2022

Screening of specific quantitative peptides of beef by LC-MS/MS coupled with OPLS-DA.

Food Chem 2022 Sep 9;387:132932. Epub 2022 Apr 9.

China Meat Research Center, 100068 Beijing, China. Electronic address:

A rapid, simple, and efficient analysis methodology for screening specific quantitative peptides of beef was established based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with orthogonal partial least squares-discriminant analysis (OPLS-DA). The OPLS-DA model was built to select species-specific peptides that make a significant contribution to classification. Peptides with statistical significance were selected based on the variable importance in the projection (VIP) values and univariate P values. After the workflow of the statistical process, three specific quantitative peptides were identified by using homemade products with different beef contents. A quantification method for selected specific quantitative peptides was established by using LC-MS/MS. The quantitative results were applied to commercialized beef products. The developed method has high sensitivity, specificity, and repeatability. The results of this study proved that the integration of LC-MS/MS coupled with OPLS-DA is an efficient method for screening specific quantitative peptides and identification of the authenticity of meat products.
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http://dx.doi.org/10.1016/j.foodchem.2022.132932DOI Listing
September 2022

Susceptibilities of ten fish cell lines to infection with Tilapia lake virus.

Microb Pathog 2022 May 11;166:105510. Epub 2022 Apr 11.

Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China. Electronic address:

Tilapia lake virus disease (TiLVD) caused by Tilapia lake virus (TiLV) is a great threat to the global tilapia culture industry. Effective prevention and control strategies have not been developed due to limited basic research of pathogenesis of TiLVD. Cell lines from different fish species have been found to be permissive to TiLV infection. In the current study, we comprehensively analyzed TiLV susceptibilities to 10 permanent growing fish cell lines. We found that the highest viral titers were generated onto TiB cells originated from the tilapia species Oreochromis mossambicus, MSF from the largemouth bass Micropterus salmoides, CAMK from the hybrid snakehead Channa argus × Channa maculata and SS derived from the perch species Siniperca chuatsi. Viral copy numbers from these four cell lines ranged from 4 × 10 copies/μL to 4.6 × 10 copies/μL. Confocal immunofluorescent microscopy also indicated that all 10 cell lines can support varying degrees of viral infection and replication. TiLV particles can be observed in cells from randomly selected three fish species using electron microscope. This study will assist in research and development of prevention and control of TiLVD.
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http://dx.doi.org/10.1016/j.micpath.2022.105510DOI Listing
May 2022

Association Between Abdominal Adipose Tissue Distribution and Obstructive Sleep Apnea in Chinese Obese Patients.

Front Endocrinol (Lausanne) 2022 24;13:847324. Epub 2022 Mar 24.

Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Purpose: Factors related to the occurrence of obstructive sleep apnea syndrome (OSAS) in obesity have not been fully clarified. The aim of this study was to identify the association between OSAS and abdominal fat distribution in a cohort of Chinese obese patients.

Methods: This cross-sectional study collected demographic data of 122 obese patients who were admitted into the in-patient unit of the Department of Endocrinology, Shanghai Tenth People's Hospital from July 2018 to January 2021. OSAS was diagnosed based on the results of overnight polysomnography, and the abdominal fat distribution was measured by bioelectrical impedance analysis (BIA). Univariate and multivariate logistic regression analyses were used to investigate the association between OSAS and the distribution of abdominal fat.

Results: (1) The mean age (SD) of the obese patients included was 32.44 (11.81) years old, and the overall incidence rate of OSAS was 51.06%. Twenty-four (25.53%) patients had mild OSAS, 10 (10.64%) had moderate OSAS, and 14 (14.89%) had severe OSAS. The apnea hypopnea index (AHI) of men was significantly higher than that of women (5.50, interquartile range (IQR) 3.80-30.6 vs. 4.2, IQR 1.4-12 events/h,  = 0.014). Meanwhile, men had a significantly higher visceral fat area when compared with women (180.29 ± 51.64 vs. 143.88 ± 53.42 cm,  = 0.002). (2) Patients with OSAS had a significantly higher waist circumference, fasting plasma glucose, 2 h postprandial plasma glucose, glycated hemoglobin, and visceral fat area than patients without OSAS (all  < 0.05). (3) AHI was significantly positively associated with BMI, neck circumference, waist circumference, and visceral fat area ( = 0.306,  = 0.003;  = 0.380,  < 0.001;  = 0.328,  = 0.002;  = 0.420,  < 0.001) but not with subcutaneous fat area ( = 0.094). Multivariate analysis demonstrated that abdominal fat area and fasting plasma glucose were independent risk factors for OSAS (odds ratio, 1.016; 95% confidence interval, 1.005-1,026,  = 0.005; odds ratio, 1.618; 95% confidence interval, 1.149-2.278,  = 0.006).

Conclusions: In obese patients, the abdominal visceral adipose deposit but not the subcutaneous fat area was associated with OSAS and was an independent risk factor for OSAS. Therefore, improving the distribution of abdominal fat may contribute to alleviating the severity of OSAS in obesity.
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http://dx.doi.org/10.3389/fendo.2022.847324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988152PMC
April 2022

Zoonotic origin of the human malaria parasite Plasmodium malariae from African apes.

Nat Commun 2022 04 6;13(1):1868. Epub 2022 Apr 6.

Institute of Evolutionary Biology and Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, EH9 3FL, UK.

The human parasite Plasmodium malariae has relatives infecting African apes (Plasmodium rodhaini) and New World monkeys (Plasmodium brasilianum), but its origins remain unknown. Using a novel approach to characterise P. malariae-related sequences in wild and captive African apes, we found that this group comprises three distinct lineages, one of which represents a previously unknown, highly divergent species infecting chimpanzees, bonobos and gorillas across central Africa. A second ape-derived lineage is much more closely related to the third, human-infective lineage P. malariae, but exhibits little evidence of genetic exchange with it, and so likely represents a separate species. Moreover, the levels and nature of genetic polymorphisms in P. malariae indicate that it resulted from the zoonotic transmission of an African ape parasite, reminiscent of the origin of P. falciparum. In contrast, P. brasilianum falls within the radiation of human P. malariae, and thus reflects a recent anthroponosis.
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http://dx.doi.org/10.1038/s41467-022-29306-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987028PMC
April 2022

The potential role and regulatory mechanism of IL-33/ST2 axis on T lymphocytes during lipopolysaccharide stimulation or perinatal Listeria infection.

Int Immunopharmacol 2022 Jul 1;108:108742. Epub 2022 Apr 1.

Department of Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, China. Electronic address:

Background: Interleukin-33 (IL-33) is a member of the interleukin-1 family, which is reported to be important across a range of diseases. However, the mechanisms underlying IL-33/ST2 axis in infectious diseases have not yet been fully addressed.

Methods: We established both lipopolysaccharide (LPS)-induced injuryin T cells and Listeria monocytogenes (Lm) infection model to determine the effect of IL-33 on infectious immunity.

Results: The T cell proliferation was inhibited by LPS while IL-33 could reverse the outcome. Further, apoptosis was significantly promoted after serum stimulation (ST)2 knockdown, suggesting IL-33, acting through its receptor ST2, may attenuate the inhibitory effect of LPS on T cells through the apoptotic signaling pathway. In this study, we also identified an IL-33-mediated mechanism of T cell differentiation in pregnant mice infected with Lm. Here, we observed the elevated expression of IL-33 in pregnant mice infected with Lm. Furthermore, we revealed that blocking IL-33 markedly decreased the abortion rate and placental bacterial load, but weakened placental inflammatory repair, by inhibiting Th2 cell-mediated immune responses and relatively intensifying Th1-dominent immunoreaction.

Conclusions: These findings reveal a previously unidentified mechanism underlying IL-33/ST2 axis. IL-33 signaling and targeting T cell-mediated immunity may present a new therapeutic strategy for the treatment of infectious diseases.
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http://dx.doi.org/10.1016/j.intimp.2022.108742DOI Listing
July 2022

Novel Angiogenic Regulators and Anti-Angiogenesis Drugs Targeting Angiogenesis Signaling Pathways: Perspectives for Targeting Angiogenesis in Lung Cancer.

Front Oncol 2022 16;12:842960. Epub 2022 Mar 16.

Pharmacy Department, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Lung cancer growth is dependent on angiogenesis. In recent years, angiogenesis inhibitors have attracted more and more attention as potential lung cancer treatments. Current anti-angiogenic drugs targeting VEGF or receptor tyrosine kinases mainly inhibit tumor growth by reducing angiogenesis and blocking the energy supply of lung cancer cells. However, these drugs have limited efficiency, raising concerns about limited scope of action and mechanisms of patient resistance to existing drugs. Therefore, current basic research on angiogenic regulators has focused more on screening carcinogenic/anticancer genes, miRNAs, lncRNAs, proteins and other biomolecules capable of regulating the expression of specific targets in angiogenesis signaling pathways. In addition, new uses for existing drugs and new drug delivery systems have received increasing attention. In our article, we analyze the application status and research hotspots of angiogenesis inhibitors in lung cancer treatment as a reference for subsequent mechanistic research and drug development.
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http://dx.doi.org/10.3389/fonc.2022.842960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965887PMC
March 2022

Glaucocalyxin A alleviates lipopolysaccharide-induced inflammation and apoptosis in pulmonary microvascular endothelial cells and permeability injury by inhibiting STAT3 signaling.

Exp Ther Med 2022 Apr 1;23(4):313. Epub 2022 Mar 1.

Pediatrics Department, Zhongshan People's Hospital, Zhongshan, Guangdong 528403, P.R. China.

Glaucocalyxin A (GLA), an ent-kauranoid diterpene derived from var. , possesses antibacterial, anti-oxidative and anti-neuroinflammatory properties. The present study aimed to investigate the potential mechanisms underlying GLA in the pathogenesis of pneumonia. Human pulmonary microvascular endothelial cells (hPMVECs) treated with lipopolysaccharide (LPS) were treated with GLA, followed by the detection of cell viability, inflammation, apoptosis and cell permeability. Furthermore, the protein expression levels of apoptosis- and permeability-associated proteins were determined using western blot analysis. Following treatment with a signal transducer and activator of transcription 3 (STAT3) activator, the protein expression levels of STAT3 and endoplasmic reticulum stress-associated proteins were determined, to confirm whether STAT3 signaling was mediated by GLA. Lastly, the mRNA expression level of inflammatory cytokines, apoptosis and permeability injury were also determined following treatment with a STAT3 activator. The results revealed that GLA ameliorated inflammation, apoptosis and permeability injury in LPS-induced hPMVECs. Following treatment with a STAT3 activator, the therapeutic effects of GLA on LPS-induced hPMVECs were abrogated. In conclusion, GLA alleviated LPS-induced inflammation, apoptosis and permeability injury in hPMVECs by inhibiting STAT3 signaling, which highlighted the potential therapeutic value of GLA in the treatment of pneumonia.
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http://dx.doi.org/10.3892/etm.2022.11242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943557PMC
April 2022

Solute carrier organic anion transporter family member 4A1 promotes colorectal cancer progression and is regulated by miR-1224-5p.

Neoplasma 2022 Mar 24. Epub 2022 Mar 24.

Department of Pharmacy, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Colorectal cancer (CRC) is one of the most common malignant tumors, and pharmacological treatments of CRC are unsatisfactory. Increasing evidence shows that solute carrier organic anion transporter family member 4A1 (SLCO4A1) is abnormally expressed in numerous cancer types and may be correlated with cancer development and metastasis. However, the roles of SLCO4A1 in CRC are incompletely understood. This study utilized the GSE110224 dataset and other databases to analyze SLCO4A1 expression levels in CRC tissues. The expression levels of SLCO4A1 in CRC cell lines were evaluated by quantitative real-time polymerase chain reaction and western blotting. The roles of SLCO4A1 in CRC cell proliferation, migration, invasion, and epithelial-mesenchymal transition were assessed. The interaction between SLCO4A1 and microRNA-1224-5p was verified using a dual-luciferase reporter assay. The effect of SLCO4A1 in vivo was investigated using a BALB/c mouse model. The level of SLCO4A1 expression was increased in CRC tissues and cell lines. Moreover, high SLCO4A1 expression was positively associated with a poor prognosis. The results of gain- and loss-of-function experiments showed that SLCO4A1 knockdown suppressed CRC cell proliferation, migration, invasion, and epithelial-mesenchymal transition while SLCO4A1 overexpression had opposite effects in vitro. Furthermore, SLCO4A1 knockdown could suppress tumor growth in vivo. Further analyses showed that SLCO4A1 was downregulated by miR-1224-5p. Rescue experiments confirmed that SLCO4A1 reversed the effect of miR-1224-5p on cell function. These results suggested that SLCO4A1 acted as an oncogene to regulate CRC development and was a potential target for CRC treatment.
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http://dx.doi.org/10.4149/neo_2022_211230N1854DOI Listing
March 2022
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