Publications by authors named "Yingying Feng"

56 Publications

Salicylic acid carboxyl glucosyltransferase UGT87E7 regulates disease resistance in Camellia sinensis.

Plant Physiol 2021 Dec 6. Epub 2021 Dec 6.

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, 230036, Hefei, Anhui, P. R. China.

Plant immune response following pathogenic infection is regulated by plant hormones, and salicylic acid (SA) and its sugar conjugates play important roles in establishing basal resistance. Here, the important pathogen Pseudopestalotiopsis camelliae-sinensis (Pcs) was isolated from tea gray blight, one of the most destructive diseases in tea plantations. Transcriptomic analysis led to the discovery of the putative Camellia sinensis UDP-glucosyltransferase CsUGT87E7 whose expression was significantly induced by SA application and Pcs infection. Recombinant CsUGT87E7 glucosylates SA with a Km value of 12 µM to form SA glucose ester. Downregulation reduced the accumulation of SA glucose ester, and CsUGT87E7-silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. Similarly, CsUGT87E7-silenced tea leaves accumulated significantly less SA after infection and showed reduced expression of pathogenesis-related genes. These results suggest that CsUGT87E7 is an SA carboxyl glucosyltransferase that plays a positive role in plant disease resistance by modulating SA homeostasis through a mechanism distinct from that described in Arabidopsis (Arabidopsis thaliana). This study provides insight into the mechanisms of SA metabolism and highlights the role of SA glucose ester in the modulation of plant disease resistance.
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http://dx.doi.org/10.1093/plphys/kiab569DOI Listing
December 2021

The immediate and long-term impacts of the COVID-19 pandemic on patients with obsessive-compulsive disorder: A one-year follow-up study.

Psychiatry Res 2021 12 2;306:114268. Epub 2021 Nov 2.

Peking University Sixth Hospital, Peking University Institute of Mental, Health, Key Laboratory of Mental Health, Ministry of Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), China. Electronic address:

Several recent publications have revealed that obsessive-compulsive disorder (OCD) patients were adversely affected during coronavirus disease 2019 (COVID-19); however, how long this negative impact will last is unclear. Our study aimed to investigate the impact of the COVID-19 pandemic on OCD patients after one year. Online questionnaires were administered, and clinical interviews were conducted to assess OCD symptoms, depression, anxiety, information about COVID-19 and mental resilience at baseline (1 December 2019-1 January 2020), during early COVID-19 (26 February-25 March 2020) and at the one-year follow-up (26 February-25 March 2021). A total of 110 OCD patients were enrolled. Our findings showed that OCD, depressive and anxiety symptoms worsened during early COVID-19, and the negative impact persisted at the one-year follow-up. Multivariate analysis showed that female gender, concern about COVID-19 and OCD symptom severity at baseline were risk factors for exacerbation of OCD symptoms during early COVID-19, while optimism, as one composite factor of resilience, was a protective factor against exacerbation of OCD symptoms both during early COVID-19 and at follow-up. Our study showed that COVID-19 had immediate and long-term impacts on the exacerbation of OCD symptoms, and interventions targeted at improving resilience are recommended.
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http://dx.doi.org/10.1016/j.psychres.2021.114268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562016PMC
December 2021

Polydopamine-coated bimetallic ZIF derivatives as an air cathode for acidic Zn-air batteries with super-high potential.

Chem Commun (Camb) 2021 Oct 26;57(85):11248-11251. Epub 2021 Oct 26.

Key Laboratory of Eco-Chemical Engineering, Taishan Scholar Advantage and Characteristic Discipline Team of Eco-Chemical Process and Technology, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

[email protected] is synthesized, which exhibits a superior ORR/OER performance in acid/base solution. Hence, acid-base dual-electrolyte-based Zn-air batteries using [email protected] reveal a long cycling life and a super-high voltage (2.1 V).
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http://dx.doi.org/10.1039/d1cc04443eDOI Listing
October 2021

Aberrant microRNA expression in B lymphocytes from patients with primary warm autoimmune haemolytic anaemia.

Autoimmunity 2021 Aug 28;54(5):264-274. Epub 2021 May 28.

Department of Hematology, General Hospital Tianjin Medical University, Tianjin, China.

Objective: To screen and analyze the micro-Ribonucleic Acid (miRNA) expression profile in B lymphocytes from patients with autoimmune haemolytic anaemia (AIHA) using high-throughput sequencing.

Methods: Twelve patients with warm autoimmune haemolytic anaemia (wAIHA) and twelve healthy controls (HCs) were enrolled. CD19 B lymphocytes were isolated and purified using magnetic activated cell sorting (MACS). RNA was subsequently extracted from these cells and a small RNA library was created. The miRNA expression profile was analyzed using Beijing Genomics Institute Sequencing 500 (BGISEQ-500), and stem-loop real-time quantitative PCR (stem-loop qRT-PCR) was used to verify the sequencing results. Downstream target genes of the differentially expressed miRNAs were predicted using miRanda and TargetScan online software, and GO functional enrichment and pathway enrichment analyses were performed on these genes.

Results: Compared with HCs, 178 upregulated and 143 downregulated miRNAs were identified in wAIHA patients, and stem-loop qRT-PCR of four randomly selected differentially expressed miRNAs verified the sequencing results. Ninety-five significantly enriched GO terms and eighty-five significantly enriched pathways were identified. Genes targeted by differentially expressed miRNAs were found to be mainly involved in the regulation of signal transduction, metabolic processes, immune reactions, and neoplastic disease development.

Conclusion: The expression of miRNAs in B lymphocytes from patients with primary wAIHA was deregulated, and this phenomenon may be involved in the pathogenesis of wAIHA.
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http://dx.doi.org/10.1080/08916934.2021.1931842DOI Listing
August 2021

Structural Characterization of an Alkali-Soluble Polysaccharide from Angelica sinensis and Its Antitumor Activity in Vivo.

Chem Biodivers 2021 Jun 11;18(6):e2100089. Epub 2021 May 11.

College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China.

A novel alkali-soluble polysaccharide (AASP) was isolated from Angelica sinensis (Oliv.) Diels under aqueous alkali treatment, and its structural characterization and antitumor activity in Vivo were evaluated in present study. Results of HPGPC and IC revealed that AASP was a neutral polysaccharide containing Ara, Gal and Glc in the mole ratio of 1.00 : 2.26 : 24.43, with the average molecular weight of 4.7 kDa. Periodate oxidation, Smith degradation, methylation, FT-IR, and NMR analyses further demonstrated that a preliminary structure of AASP was proposed as follows: (1→3)-linked arabinose, (1→6)-linked galactose, and (1→), (1→4), (1→6), (1→3,6)-linked glucose with α- and β-configuration. In Vivo antitumor assays, AASP exhibited prominent antitumor effects on H22 hepatoma cells with an inhibitory ratio of 48.57 % and effectively protected thymuses and spleens of tumor-bearing mice. Besides, AASP displayed a proliferation stimulating activity of immunocytes (splenocytes, peritoneal macrophages and natural killer cells), and an auxo-action for cytokines release (TNF-α, IL-2 and IFN-γ), leading to the apoptosis of H22 solid tumors cells via G0/G1 phase arrested. The above data demonstrated that AASP holds great application potential to be a safe and effective antitumor supplement in the future.
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http://dx.doi.org/10.1002/cbdv.202100089DOI Listing
June 2021

M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs.

J Mater Sci Mater Med 2020 Nov 28;31(12):124. Epub 2020 Nov 28.

Foshan Stomatological Hospital, School of Stomatology and Medicine, Foshan University, Foshan, 528225, PR China.

As traditional root canal obturation leads to the loss of the biological activity of the tooth, it is necessary to develop a material that promotes the regeneration of dental tissue. However, this remains a challenging task. Our study aims to construct a mineralized material to support the proliferation and differentiation of dental pulp stem cells (DPSCs), and to explore a new strategy for the treatment of pulp tissue necrosis. Mineralized keratin (M-keratin), defined as keratin that has been mineralized in simulated body fluid, was first harvested to construct the root canal filling material. Characterizations indicated that new substances or components were formed on the surface of keratin particles after mineralization, and the morphology of the keratin was changed. M-keratin promoted the growth, proliferation, and differentiation of DPSCs. After cultivation with M-keratin, DPSCs exhibited more extracellular matrix proteins interacting with the culture interface, the number of these cells increased significantly, and the 3-[4,5-dimethylthiazol-2-yl-]-2,5-diphenyltetrazolium bromide values of cells in the experimental group also increased. Meanwhile, signs that the DPSCs began to differentiate into odontoblasts were observed or detected by alizarin red S staining, ELISA, RNA-Seq, and western blot. We hope that this study will contribute to the development of a new material that promotes the regeneration of dental tissue as well as providing new ideas and strategies for the treatment of dental pulp disease.
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http://dx.doi.org/10.1007/s10856-020-06465-8DOI Listing
November 2020

Sustainable production and biomedical application of polymalic acid from renewable biomass and food processing wastes.

Crit Rev Biotechnol 2021 Mar 5;41(2):216-228. Epub 2020 Nov 5.

William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.

Polymalic acid (PMA), a homopolymer of L-malic acid (MA) generated from a yeast-like fungus , has unique properties and many applications in food, biomedical, and environmental fields. Acid hydrolysis of PMA, releasing the monomer MA, has become a novel process for the production of bio-based MA, which currently is produced by chemical synthesis using petroleum-derived feedstocks. Recently, current researches attempted to develop economically competitive process for PMA and MA production from renewable biomass feedstocks. Compared to lignocellulosic biomass, PMA and MA production from low-value food processing wastes or by-products, generated from corn, sugarcane, or soybean refinery industries, showed more economical and sustainable for developing a MA derivatives platform from biomass biorefinery to chemical conversion. In the review, we compared the process feasibility for PMA fermentation with lignocellulosic biomass and food process wastes. Some useful strategies for metabolic engineering are summarized. Its changeable applicability and future prospects in food and biomedical fields are also discussed.
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http://dx.doi.org/10.1080/07388551.2020.1844632DOI Listing
March 2021

Increased physiological dead space in mechanically ventilated COVID-19 patients recovering from severe acute respiratory distress syndrome: a case report.

BMC Infect Dis 2020 Aug 27;20(1):637. Epub 2020 Aug 27.

Department of Pulmonary and Critical Care Medicine, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, China-Japan Friendship Hospital, No 2, East Yinghua Road, Chaoyang District, Beijing, 100029, China.

Background: An ongoing outbreak of coronavirus disease 2019 (COVID-19) is spreading globally. Recently, several articles have mentioned that the early acute respiratory distress syndrome (ARDS) caused by COVID-19 significantly differ from those of ARDS due to other causes. Actually, we newly observed that some mechanically ventilated COVID-19 patients recovering from severe ARDS (more than 14 days after invasive ventilation) often experienced evidently gradual increases in CO retention and minute ventilation. However, the underlying mechanics remain unclear.

Case Presentation: To explain these pathophysiological features and discuss the ventilatory strategy during the late phase of severe ARDS in COVID-19 patients, we first used a metabolic module on a General Electric R860 ventilator (Engstrom Carestation; GE Healthcare, USA) to monitor parameters related to gas metabolism, lung mechanics and physiological dead space in two COVID-19 patients. We found that remarkably decreased ventilatory efficiency (e.g., the ratio of dead space to tidal volume 70-80%, arterial to end-tidal CO difference 18-23 mmHg and ventilatory ratio 3-4) and hypermetabolism (oxygen consumption 300-400 ml/min, CO elimination 200-300 ml/min) may explain why these patients experienced more severe respiratory distress and CO retention in the late phase of ARDS caused by COVID-19.

Conclusion: During the recovery period of ARDS among mechanically-ventilated COVID-19 patients, attention should be paid to the monitoring of physiological dead space and metabolism. Tidal volume (8-9 ml/kg) could be increased appropriately under the limited plateau pressure; however, barotrauma should still be kept in mind.
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http://dx.doi.org/10.1186/s12879-020-05360-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450943PMC
August 2020

Transbronchial lung cryobiopsy may be of value for nonresolving acute respiratory distress syndrome: case series and systematic literature review.

BMC Pulm Med 2020 Jun 29;20(1):183. Epub 2020 Jun 29.

Department of Pulmonary and Critical Care Medicine, Centre of Respiratory Medicine, China-Japan Friendship Hospital; National Clinical Research Centre for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, 2 Yinghuayuan East Street, Chaoyang District, Beijing, 100029, China.

Background: Identification of pathologic features is helpful for the management of nonresolving acute respiratory distress syndrome (ARDS). Transbronchial lung cryobiopsy (TBLC) is a novel biopsy technique that may have comparable utility to surgical biopsy. The aim of this study was to assess the value of TBLC in patients with nonresolving ARDS.

Methods: All patients with nonresolving ARDS who underwent TBLC from January 2019 to August 2019 in a tertiary medical ICU were included. In addition, a literature search of TBLC for ARDS was performed by searching PubMed, EMBASE, ATS/ERS/APSR meeting abstracts, ClinicalTrials.gov , and Google Scholar. Data on complications, histologic diagnosis, management changes, and outcomes were analysed.

Results: Five patients (three women and two men) underwent TBLC. None of the patients developed pneumothorax, although two patients developed massive bleeding, which was controlled by continuous occlusion using bronchial blockers. There were no procedure-related deaths. Diffuse alveolar damage (DAD) and alternative histologic patterns were found in two and three patients, respectively, resulting in management changes in all cases. The literature search yielded four studies, which together with the present study comprised data from 25 cases in which TBLC was used in nonresolving ARDS. The summary diagnostic yield was 92% (23/25). Only 44% (11/25) of cases were proven to be DAD. TBLC contributed to management changes in 80% of patients (20/25). Procedure-related complications consisted of pneumothorax (16%, 4/25), significant bleeding (12%, 3/25), and persistent air leaks (8%, 2/25). There were no procedure-related deaths. The follow-up survival rate was 61.9% (13/21).

Conclusions: The complications of TBLC in selected patients with nonresolving ARDS may be acceptable. The procedure may have a high diagnostic yield and can lead to a re-evaluation of the diagnosis as well as changes in patient management. Further investigations with larger sample sizes are required.
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http://dx.doi.org/10.1186/s12890-020-01203-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322907PMC
June 2020

NiFe-coordinated zeolitic imidazolate framework derived trifunctional electrocatalyst for overall water-splitting and zinc-air batteries.

J Colloid Interface Sci 2020 Nov 15;579:1-11. Epub 2020 Jun 15.

Key Laboratory of Colloid & Interface Chemistry (Ministry of Education), Shandong University, Jinan 250100, China.

Developing high-efficient non-noble metal electrocatalysts toward oxygen reduction reaction (ORR), oxygen evolution reaction (OER), hydrogen evolution reaction (HER), water-splitting, and the zinc-air battery is essential but challenging. Zeolitic imidazole frameworks (ZIFs) are generally employed as ideal platforms for the design and fabrication of energy-related catalysts by exploiting their porous structure with high surface area and flexibility. This work presents the preparation of NiFe-bimetallic species decorated N-doped porous carbon composite ([email protected]) through pyrolyzing the NiFe-coordinated ZIF precursor. The obtained [email protected] shows a larger surface area and porous nanostructure comprising the active bimetallic species evenly distributed in the conductive carbon matrix. The nanocomposite demonstrates excellent trifunctional catalytic activity toward ORR, OER, and HER. For ORR, [email protected] offers a half-wave potential value of 0.87 V, which is positively shifted by 30 mV relative to that of Pt/C in 1 M KOH. [email protected] exhibits OER activity with superior overpotential, reaction kinetics, and durability to those of IrO. It also demonstrates the desirable HER activity with a low overpotential of 150 mV at 10 mA/cm and excellent durability in an acidic electrolyte. Additionally, the water-splitting configuration and zinc-air battery assembled with [email protected] catalyst reveal superior performance to noble-metal catalysts. Such excellent electrocatalytic performance can be attributed to the distinct chemical composition of evenly distributed bimetallic active sites on highly conductive carbon sheets, and the porous nanostructure with large surface area and fast mass transfer.
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http://dx.doi.org/10.1016/j.jcis.2020.06.052DOI Listing
November 2020

Characteristics of patients with autoimmune haemolytic anaemia secondary to lymphoproliferative disorder: A single-centre retrospective analysis.

Sci Rep 2019 12 23;9(1):19716. Epub 2019 Dec 23.

Haematology Department of General Hospital, Tianjin Medical University, Tianjin, 300052, China.

Autoimmune haemolytic anaemia (AIHA) is a kind of autoimmune diseases characterized by autoantibodies which produced and secreted by abnormal activated B lymphocytes directed against red blood cells (RBC). Study reveals that about 50% AIHA mainly occurs secondary to lymphoproliferative disorders (LPD) and autoimmune diseases. In this study, we aim to explore the characteristics of patients with AIHA secondary to LPD. Fifteen patients with AIHA secondary to LPD (secondary group) and 60 with primary AIHA (primary group) were retrospectively included. Patients in the secondary group [(59.40 ± 4.74) y] were older than those in the primary group [(47.53 ± 2.30) y] (p = 0.024). Reticulocyte counts were lower for the secondary group [(134.55 ± 20.67) × 10/L] than for the primary group [(193.88 ± 27.32) × 10/L] (p = 0.09). Haptoglobin was higher in the secondary (0.75 ± 0.19) g/L than in the primary group (0.34 ± 0.05) g/L (p = 0.004). The ratio of CD3CD4/CD3CD8 was higher in the secondary (1.81 ± 0.41) than in the primary (1.05 ± 0.12) group (p = 0.025). Duration of remission was shorter in the secondary [(23.52 ± 5.20) months] than in the primary [(40.87 ± 3.92) months] group (p = 0.013). Relapse rate was higher for the secondary (33.3%) than for the primary (8.3%) group (p = 0.003). Mortality rate was higher in the secondary (33.3%) than in the primary (8.3%) group (p = 0.003). Progression-free survival was shorter in the secondary than in the primary group (p = 0.021). In conclusion, patients with AIHA secondary to LPD showed higher age at diagnosis, shorter remission time, and higher recurrence and mortality rates than did those with primary AIHA.
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http://dx.doi.org/10.1038/s41598-019-56162-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928151PMC
December 2019

In situ encapsulation of Co-based nanoparticles into nitrogen-doped carbon nanotubes-modified reduced graphene oxide as an air cathode for high-performance Zn-air batteries.

Nanoscale 2019 Nov;11(45):21943-21952

State Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Exploring highly efficient catalysts for the oxygen reduction/evolution reaction (ORR/OER) is very important in rechargeable Zn-air batteries. N-doped carbon coupled with transition metal-based species are among the most promising cathode catalysts for Zn-air batteries. However, the aggregation of metal-based sites during the synthetic/cycling process is a serious drawback of these catalysts. Herein, in situ encapsulation of ultra-small Co/Co4N nanoparticles into N-doping carbon nanotubes (N-CNTs) anchored on reduced GO (Co/[email protected]/rGO) has been achieved through pyrolyzing a core-shell-structured [email protected] GO ([email protected]/GO) precursor; the nanoparticles have been further applied as a bifunctional catalyst in Zn-air batteries. Benefitting from its uniform dispersion of Co-based particles, close contact of Co/Co4N species and N-CNTs, and high N content, Co/[email protected]/rGO shows outstanding catalytic activity/stability towards ORR and OER. Moreover, Zn volatilization and rGO introduction in Co/[email protected]/rGO can effectively promote the reactions of Zn-air cells. Hence, the Co/[email protected]/rGO-based conventional Zn-air battery exhibits a fantastic specific capacity of 783 mA h gZn-1, a continuous discharge platform over 6 days, a high-power density of ∼200 mW cm-2 and an ultra-long cycling life of 440 h with a small overpotential of ∼0.8 V. Moreover, a flexible Co/[email protected]/rGO-based Zn-air cell was also designed and revealed outstanding mechanical flexibility and good electrochemical performance, which suggests its potential application prospects in wearable electronic devices.
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http://dx.doi.org/10.1039/c9nr07270eDOI Listing
November 2019

Antitumor effects of seleno-β-lactoglobulin on human breast cancer MCF-7 and MDA-MB-231 cells in vitro.

Toxicol In Vitro 2019 Dec 23;61:104607. Epub 2019 Jul 23.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address:

Seleno-β-lactoglobulin (Se-β-Lg) was synthesized using seleninic acid, an organoselenium compound, and β-lactoglobulin (β-Lg), an important component of milk. Previously, we have studied the effects of Se-β-Lg on hepatocellular carcinoma and gastric cancer cells. In this study, we investigated the antitumor effects of Se-β-Lg and its potential mechanisms of action against human breast cancer cells (MCF-7 and MDA-MB-231). The results showed that the half-maximal inhibitory concentrations (IC50) of Se-β-Lg were 40.84 μg/mL for MCF-7 cells and 46.04 μg/mL for MDA-MB-231 cells at 24 h, while the compound showed no cytotoxicity to normal breast cells. The involvement of reactive oxygen species (ROS) in the activation of the apoptotic signaling pathway by Se-β-Lg was demonstrated by the incubation of cells with 80 μg/mL Se-β-Lg and determination of the rates of apoptosis and intracellular ROS levels after the addition of 10 mM N-acetyl-l-cysteine, a ROS inhibitor. Our findings revealed highly potent anticancer activities of Se-β-Lg against breast cancer cells and suggested that the compound may be used as a chemopreventive agent for breast cancer. Furthermore, we thoroughly elucidated the antitumor mechanism of Se-β-Lg.
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http://dx.doi.org/10.1016/j.tiv.2019.104607DOI Listing
December 2019

The Optimal Distance of the Electrode to the Lifting Film Surface when Lifting Dust Footwear Impressions Using an Electrostatic Dust Print Lifter.

J Forensic Sci 2019 Nov 25;64(6):1873-1877. Epub 2019 Jun 25.

Department of Forensic Science and Technology, Xinjiang Police College, Urumqi, 830011, China.

Footwear impressions are one of the valuable physical evidence encountered at crime scenes and its identification can facilitate narrowing down the suspects and establishing the identity of the criminals. The technique of electrostatic lifting (ESL) dust shoeprints at crime scenes is well established with scenes of crime examiners. And in the procedure, the recovery of the original item containing the impression should be made as good as it can and not damage the marks. In this study, the different particle sizes of SiO were used to simulate dust and light soil residues, comparing different particle sizes and electrode positions were used to compare the adsorption ability on the surface of electrostatic lifting film in high-voltage electrostatic field for the collection of shoeprints. The results indicated that lifting film in electrostatic field will be pressed down to the dust surface, the compaction and electrostatic adsorption force will vary with the distance between the high-voltage electrode and film surface. Reducing the distance can increase the electric field strength near the lifting film, and the adsorption capacity of Mylar aluminum-plating film to dusts can be improved significantly. Adsorption capacity of the lifting film was related to the size of dust particles and significantly improved with the increase of the particle charge. The optimum distance which can get the best adsorption capacity between the electrode and film surface is from 10 to 15 mm.
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http://dx.doi.org/10.1111/1556-4029.14116DOI Listing
November 2019

Neurological soft signs and neurocognitive deficits in remitted patients with schizophrenia, their first-degree unaffected relatives, and healthy controls.

Eur Arch Psychiatry Clin Neurosci 2020 Apr 23;270(3):383-391. Epub 2019 May 23.

Wuhan Mental Health Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, China.

Neurological soft signs (NSS) and neurocognitive deficits (ND) are highly prevalent in schizophrenia, and have been separately proposed as candidate endophenotypes of schizophrenia. However, few relevant studies focus on remitted patients with schizophrenia (RP) and integrate NSS and ND as a composite endophenotype. This study aimed to explore the NSS and ND and examine the comparative relationship between them in RP, their first-degree unaffected relatives (FDR), and healthy controls, furthermore, to seek potential endophenotypes subitems of NSS and ND and create a composite endophenotype. 86 RP, 86 FDR, and 86 healthy controls were included. NSS and ND were independently assessed with Cambridge Neurological Inventory and MATRICS Consensus Cognitive Battery. RP had more NSS and ND than FDR in all subitems except disinhibition, information processing speed, working memory, and visual memory. Similarly, FDR presented poorer performance than controls in all subscales except disinhibition, sensory integration, working memory, and visual memory. Six subitems of NSS and ND met the criteria of endophenotype and the three groups were most accurately classified (71.2%) with these subitems working as a composite endophenotype. Moreover, information processing speed, attention, and social cognition were associated with sensory integration in RP and FDR. These findings add evidences that certain subitems of NSS and ND might be the endophenotypes of schizophrenia and integrating these endophenotypes may prove useful in identifying schizophrenia and high-risk individuals. Furthermore, sensory integration and specific cognitive domains covary, hence suggesting an overlap of compromised underlying neural systems.
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http://dx.doi.org/10.1007/s00406-019-01024-xDOI Listing
April 2020

Structural Characterization and Antitumor Activity of Polysaccharides from L.

Oxid Med Cell Longev 2018 30;2018:9579262. Epub 2018 Dec 30.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

The water-soluble polysaccharides from L. (KGPs) were extracted and purified, and their structural characteristics and antitumor activity were further investigated. The UV spectrum, high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FTIR), and ion chromatography (IC) were employed to evaluate the structural characteristics, and H22 tumor-bearing mice model was established to demonstrate the antitumor activity. Physicochemical analysis and UV spectrum results showed that the proportions of total sugar, protein, and uronic acid in KGPs were 85.23%, 0.54%, and 24.17%, respectively. HPGPC, FTIR, and IC indicated that KGPs were acidic polysaccharides with skeletal modes of pyranose rings and mainly composed of arabinose and galactose with the average molecular weight of 8.5 × 10 Da. The antitumor experiments showed that KGPs could effectively protect the thymus and spleen of tumor-bearing mice from solid tumors and enhance the immunoregulatory ability of CD4 T cells, the cytotoxic effects of CD8 T cells and NK cells, and finally resulting in the inhibitory effects on H22 solid tumors. This study provided a theoretical foundation for the practical application of KGPs in food and medical industries.
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http://dx.doi.org/10.1155/2018/9579262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332924PMC
February 2019

Invasive pulmonary aspergillosis in patients with influenza infection: A retrospective study and review of the literature.

Clin Respir J 2019 Apr 18;13(4):202-211. Epub 2019 Mar 18.

Department of Pulmonary and Critical Care Medicine, Centre for Respiratory Diseases, China-Japan Friendship Hospital, Beijing, P.R. China.

Introduction: There has been a rapid increase in the number of influenza and invasive pulmonary aspergillosis (IPA) co-infection.

Objectives: To explore the risk factors and predictors of a poor prognosis in influenza and IPA co-infection.

Methods: We included patients with confirmed influenza during the 2017-2018 influenza season and cases of influenza and IPA co-infection in the literature.

Results: A total of 64 patients with influenza infection were admitted to ICU. Of these patients, 18 were co-infected with IPA. Others were assigned to the control group (n = 46). A total of 45 patients from the literature were added to the IPA group (n = 63). A multivariate logistic regression suggested that influenza patients who were given steroids after ICU admission, who had a white blood count (WBC) of more than 10*10 /L on ICU admission and whose CT findings manifested as multiple nodules and cavities might have a higher risk of developing IPA. Compared to survivors, non-survivors had higher sequential organ failure assessment (SOFA) scores (16 ± 4 points vs 8 ± 4 points, P < 0.001), lower CD4 T cell counts on ICU admission [315 (83-466) cells/μL vs 152 (50-220) cells/μL, P = 0.031] and more requirement extracorporeal membrane oxygenation (ECMO) support [13 (50%) vs 7 (18.9%), P = 0.015].

Conclusions: Influenza patients who are given steroids after ICU admission, who have WBCs of greater than 10*10 /L on ICU admission, and whose CT imaging shows multiple nodules and cavities might have a high risk of IPA. Higher SOFA scores, CD4 T cell counts lower than 200 cells/μL on ICU admission and more ECMO requirement might be predictors of a poor prognosis.
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http://dx.doi.org/10.1111/crj.12995DOI Listing
April 2019

Fe O /reduced graphene oxide-carbon nanotubes composite for the magnetic solid-phase extraction and HPLC determination of sulfonamides in milk.

J Sep Sci 2019 Mar 13;42(5):1058-1066. Epub 2019 Feb 13.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Hubei Province, Wuhan, P. R. China.

A novel magnetic adsorbent Fe O /reduced graphene oxide-carbon nanotubes, was prepared by one-pot solvothermal synthesis method. It was characterized by scanning electron microscopy, X-ray powder diffraction and vibrating sample magnetometry. The diameter of Fe O microparticles was about 350 nm, which were covered by carbon nanotubes and reduced graphene oxide sheets, while carbon nanotubes inserted between the reduced graphene oxide sheets effectively prevented their aggregation. The composite had large surface area and good magnetic property, suiting for magnetic solid-phase extraction and the determination of sulfonamides, by coupling with high-performance liquid chromatography. Under the optimized conditions (including extraction time, amount of adsorbent, solution pH, ionic strength and desorption conditions), a good linear was achieved in the concentration range of 5-500 μg/L and the low limits of detection and low limits of quantification were 0.35-1.32 and 1.16-4.40 μg/L, respectively. The enrichment factors were estimated to be 24.72 to 30.15 fold. The proposed method was applied for the detection of sulfonamides in milk sample and the recoveries were 88.4-105.9%, with relative standard deviations of 0.74-5.38%.
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http://dx.doi.org/10.1002/jssc.201801177DOI Listing
March 2019

Apoptosis of human gastric carcinoma MGC-803 cells induced by a novel Astragalus membranaceus polysaccharide via intrinsic mitochondrial pathways.

Int J Biol Macromol 2019 Apr 31;126:811-819. Epub 2018 Dec 31.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:

In our previous study, a novel cold-water-soluble polysaccharide (APS4) was isolated from Astragalus membranaceus. This study aimed to evaluate the proliferation inhibition and apoptosis-induced effects of APS4 on human gastric carcinoma MGC-803 cells and to investigate its potential molecular mechanism. It was found that APS4 could significantly suppress the proliferation of MGC-803 cells in a concentration- and time-dependent manner. Morphologic observations and Annexin V-FITC/PI staining showed that APS4-treated MGC-803 cells exhibited typical morphological characteristics of apoptosis. Cell cycle detection revealed that APS4 could arrest MGC-803 cells in S phase of the cell cycle. Additionally, APS4 treatment could induce the mitochondria-dependent apoptosis, which was closely related to the accumulation of intracellular ROS, the collapse of mitochondrial membrane potential, the increase of the pro-apoptotic/anti-apoptotic (Bax/Bcl-2) ratios, the release of cytochrome c, further activating the expression of caspase-9/-3 and the cleavage of poly-ADP-ribose polymerase (PARP) in MGC-803 cells. Taken together, our results suggested that APS4 had observable apoptosis-induced effects on MGC-803 cells via arresting the cell cycle in S phase and inducing the intrinsic mitochondrial apoptosis pathway.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.12.268DOI Listing
April 2019

Preparation of gene drug delivery systems of cationic peptide lipid with 0G-PAMAM as hydrophilic end and its biological properties evaluation.

Chem Phys Lipids 2019 11 9;224:104685. Epub 2018 Oct 9.

School of Biosciences and Biophamaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

As an efficient gene delivery, non-viral vectors should have high transfection efficiency, excellent endosomal escape, low cytotoxicity, and the ability to rapidly release the gene into the cytoplasm.Cationic liposome have been widely used as efficient gene carriers, but the cytotoxicity, rapid degradation and low cellular uptake are major drawback impeding its further appolication. Herein, with double lauric acid as hydrophobic chains, tartaric acid as skeleton, 0 generation PAMAM modified with lysine as hydrophilic head, a new type cationic peptide lipid was synthetised. The alkyl chain promote lipid across cell membranes and with membrane fusion, 0 generation PAMAM modified with lysine hydrophilic end amino can contain a large number of protons which can change into ammonium and combine with the DNA negatively charge phosphate groups. It is expected that this carrier has low toxicity, high transfection efficiency and targeting property. By adjusting the cationic liposome/gene weight ratio, the transfection system was optimized to improved gene transfection efficiency, reduce cytotoxicity, and increase property and stability, etc.
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http://dx.doi.org/10.1016/j.chemphyslip.2018.09.009DOI Listing
November 2019

Fresh versus frozen embryo transfer for full-term singleton birth: a retrospective cohort study.

J Ovarian Res 2018 Jul 16;11(1):59. Epub 2018 Jul 16.

The Reproduction Center, The Third Affiliated Hospital of Zhengzhou University, 7 Kangfuqian Road, Zhengzhou, 450052, Henan, People's Republic of China.

Background: Improvements in vitrification and frozen embryo transfer (FET) technologies have rapidly increased, and some evidence suggests that FET may increase pregnancy rates and lead to more favourable perinatal outcomes. However, the outcome of interest should be offspring safety. Therefore, the primary objective of our study was to investigate whether FET was preferable to fresh embryo transfer (ET) in terms of full-term neonatal birthweight and congenital malformations.

Methods: This was a retrospective cohort study of patients with no pregnancy-related complications who underwent first fresh ETs (n = 2059) or FETs (n = 2053), resulting in full-term singletons births. Outcome measures were neonatal birthweight, low birthweight (LBW), small-for-gestational age (SGA), large-for-gestational age (LGA), macrosomia and congenital malformations. Additionally, we used logistic regression to adjust for baseline characteristics (age, BMI, No. of embryos transferred and embryo stage) between the two groups.

Results: The mean neonatal birthweight was higher for singletons born after FET than for singletons born after fresh ET (3468.7 ± 475.3 vs. 3386.7 ± 448.1; p < 0.001). The frequencies of full-term singleton LBW and SGA after FET were significantly lower than those after fresh ET (1.7% vs. 3.0 and 4.4% vs. 6.7%, respectively), with adjusted rate ratios of 0.59 (95% CI, 0.37 to 0.98; p = 0.026) and 0.73 (95% CI, 0.55 to 0.99; p = 0.041), respectively. FET resulted in higher frequencies of macrosomia and LGA (15.1% vs 10.2 and 22.8% vs. 17.5%, respectively) than fresh ET, with adjusted rate ratios of 1.43 (95% CI, 1.16 to 1.75; p = 0.001) and 1.26 (95% CI, 1.07 to 1.49; p = 0.007), respectively. Furthermore, the incidence of congenital malformations was not different between the two groups (1.2% vs. 0.9%), with a rate ratio of 0.288.

Conclusions: After the cycles with pregnancy-related complications were excluded and after adjustments for baseline characteristics, women undergoing FET were associated with a higher neonatal birthweight than women undergoing fresh ET cycles. Additionally, the FET protocol was associated with lower rates of LBW and SGA and higher rates of macrosomia and LGA than the fresh ET protocol. Meanwhile, no difference in the congenital malformation rate was evident between the two groups.
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http://dx.doi.org/10.1186/s13048-018-0432-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048709PMC
July 2018

HO and NADPH oxidases involve in regulation of 2-(2-phenylethyl)chromones accumulation during salt stress in Aquilaria sinensis calli.

Plant Sci 2018 Apr 8;269:1-11. Epub 2018 Jan 8.

Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, PR China. Electronic address:

2-(2-Phenylethyl)chromones are the main compounds responsible for the quality of agarwood, which is widely used in traditional medicines, incenses and perfumes. HO and NADPH oxidases (also known as respiratory burst oxidase homologs, Rbohs) mediate diverse physiological and biochemical processes in environmental stress responses. However, little is known about the function of HO and NADPH oxidases in 2-(2-phenylethyl)chromones accumulation. In this study, we found that salt stress induced a transient increase in content of HO and 2-(2-phenylethyl)chromones accumulation in Aquilaria sinensis calli. Exogenous HO remarkably decreased the production of 2-(2-phenylethyl)chromones, while dimethylthiourea (DMTU), a scavenger of HO, significantly increased 2-(2-phenylethyl)chromones accumulation in salt treated calli. Three new HO-generating genes, named AsRbohA-C, were isolated and characterized from A. sinensis. Salt stress also induced a transient increase in AsRbohA-C expression and NADPH oxidase activity. Furthermore, exogenous HO increased AsRbohA-C expression and NADPH oxidase activity, while DMTU inhibited AsRbohA-C expression and NADPH oxidase activity under salt stress. Moreover, diphenylene iodonium (DPI), the inhibitor of NADPH oxidases, reduced AsRbohA-C expression and NADPH oxidase activity, but significantly induced 2-(2-phenylethyl)chromones accumulation during salt stress. These results clearly demonstrated the central role of HO and NADPH oxidases in regulation of salt-induced 2-(2-phenylethyl)chromones accumulation in A. sinensis calli.
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http://dx.doi.org/10.1016/j.plantsci.2018.01.002DOI Listing
April 2018

A novel mechanism of tumor-induced thymic atrophy in mice bearing H22 hepatocellular carcinoma.

Cancer Manag Res 2018 5;10:417-424. Epub 2018 Mar 5.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

Background: Thymic atrophy was discovered in tumor-bearing mice in recent years.

Methods: Flow cytometry was carried out including Annexin V-FITC/PI double staining, PI staining, Terminal dUTP nick-end labeling, CD3-FITC/CD19-PE and CD8-FITC/CD4-PE double staining. Enzyme-linked immunosorbent assay and polymerase chain reaction were also investigated.

Results: According to our experiments, we demonstrated that no signs of apoptosis in thymocytes were found in H22-bearing mice, while the proportions of CD4 T cells and CD8 T cells in thymuses were remarkably increased, the opposite tendency was found in peripheral bloods, and only CD3CD8 T cells were discovered in H22 solid tumors. We further discovered that the level of thymosin alpha 1 (Tα1) and the expression of in thymus of H22-bearing mice were significantly improved than control, which indicated the active proliferation and differentiation of thymocytes. Our study revealed that CD8 T cells could not effectively eliminate H22 cells independently when CD4 T cells were suppressed by tumors, while the body would only enhance the differentiation and maturation of T cells in thymuses and release them to solid tumor to reinforce antitumor immunocompetence, leading to a vicious cycle which finally led to thymic atrophy.

Conclusion: Our data propose a novel mechanism of tumor-induced thymic atrophy regulated by abnormal immunoreaction and may provide new ideas for the immunotherapy of tumors.
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http://dx.doi.org/10.2147/CMAR.S157512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842769PMC
March 2018

Contrast-enhanced computerized tomography combined with a targeted nanoparticle contrast agent for screening for early-phase non-small cell lung cancer.

Exp Ther Med 2017 Nov 19;14(5):5063-5068. Epub 2017 Sep 19.

Department of Radiology, The First Hospital of Shijiazhuang City, Shijiazhuang, Hebei 050011, P.R. China.

Non-small cell lung cancer (NSCLC) is a major cause of morbidity and mortality, and patients with NSCLC are frequently diagnosed at an advanced stage. This is primarily due to a lack of advanced and sensitive protocols for the detection of early stage NSCLC. Therefore, methods for the accurate diagnosis of early stage NSCLC are urgently required to improve survival rates. The present study investigated the use of contrast-enhanced computerized tomography (CECT) combined with a targeted nanoparticle contrast agent (TNCA) to diagnose early-stage NSCLC in a mice xenograft model. The TNCA used was lenvatinib, a multi-target tyrosine kinase inhibitor that inhibits vascular endothelial growth factor receptor 1-3, fibroblast growth factor receptor 1-4, platelet-derived growth factor receptor β, proto-oncogene tyrosine-protein kinase receptor Ret and mast/stem cell growth factor receptor Kit. Xenograft NSCLC mice were established and used to analyze the efficacy of CECT-TNCA compared with CT scanning alone. The TNCA was inhaled with the use of an atomizer. The results demonstrated that CECT-TNCA improved the sensitivity of the diagnosis of early stage NSCLC. In addition, imaging using the TNCA enabled the visualization of nodules in the lung in mice with early stage NSCLC. In addition, lung nodule signal enhancement was increased in CECT-TNCA compared with CT, suggesting a high accurate accumulation of the TNCA in tumor nodules. Mice diagnosed with early stage NSCLC exhibited a higher eradication rate of NSCLC after treatment with cisplatin compared with mice with advanced stage NSCLC. These data indicate that the sensitivity and accuracy of CT imaging for the diagnosis of early stage NSCLC was improved through combination with the liposome-encapsulated TNCA.
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http://dx.doi.org/10.3892/etm.2017.5140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704269PMC
November 2017

miR-30a-5p suppresses breast tumor growth and metastasis through inhibition of LDHA-mediated Warburg effect.

Cancer Lett 2017 08 29;400:89-98. Epub 2017 Apr 29.

Department of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, China. Electronic address:

Lactate dehydrogenase A (LDHA), a key enzyme regulating aerobic glycolysis, is overexpressed in many human cancers, and correlates with poor clinical outcomes. Aerobic glycolysis is a hallmark of cancer, and drugs targeting its regulators, including LDHA, are being developed. However, the mechanisms of LDHA inhibition and the physiological significance of the LDHA inhibitors in cancer cells are unclear. Here, we show that microRNA-30a-5p (miR-30a-5p) suppresses LDHA expression by directly targeting its 3'-UTR. Through inhibition of LDHA, miR-30a-5p dampens glycolysis by decreasing glucose uptake, lactate production, ATP generation, and extracellular acidification rate (ECAR), and increasing oxygen consumption rate (OCR) in breast cancer cells. Importantly, glycolysis regulated by miR-30a-5p is critical for its regulating breast tumor growth and metastasis both in vitro and in vivo. In breast cancer patients, miR-30a-5p expression is negatively correlated with LDHA expression. Moreover, patients who had increased glucose uptake in tumors assessed by PET scans showed decreased miR-30a-5p expression and increased expression of LDHA. Our findings provide clues regarding the role of miR-30a-5p as a tumor suppressor in breast cancer through the inhibition of LDHA. Targeting LDHA through miR-30a-5p could be a potential therapeutic strategy in breast cancer.
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http://dx.doi.org/10.1016/j.canlet.2017.04.034DOI Listing
August 2017

[Treatment of complications after laparoscopic intersphincteric resection for low rectal cancer].

Zhonghua Wei Chang Wai Ke Za Zhi 2017 Apr;20(4):432-438

Department of Colo-Rectal Disease Surgery, The General Hospital of the PLA Rocket Force, Beijing 100088, China.

Objective: To summarize the perioperative and postoperative complications follow laparoscopic intersphincteric resection (LapISR) in the treatment of low rectal cancer and their management.

Methods: An observational study was conducted in 73 consecutive patients who underwent LapISR for low rectal cancer between June 2011 and February 2016 in our hospital. The clinicopathological parameters, perioperative and postoperative complications, and clinical outcomes were collected from a prospectively maintained database. Perioperative and postoperative complications were defined as any complication occurring within or more than 3 months after the primary operation, respectively.

Results: Forty-nine(67.1%) cases were male and 24(32.9%) were female with a median age of 61(25 to 79) years. The median distance from distal tumor margin to anal verge was 4.0(1.0 to 5.5) cm. The median operative time was 195 (120 to 360) min, median intra operative blood loss was 100 (20 to 300) ml, median number of harvested lymph nodes was 14(3 to 31) per case. All the patients underwent preventive terminal ileum loop stoma. No conversion or hospital mortality was presented. The R0 resection rate was 98.6% with totally negative distal resection margin. A total of 34 complication episodes were recorded in 21(28.8%) patients during perioperative period, and among which 20.6%(7/34) was grade III(-IIII( according to Dindo system. Anastomosis-associated morbidity (16.4%,12/73) was the most common after LapISR, including mucosa ischemia in 9 cases(12.3%), stricture in 7 cases (9.6%, 4 cases secondary to mucosa necrosis receiving anal dilation), grade A fistula in 3 cases (4.1%) receiving conservative treatment and necrosis in 1 case (1.4%) receiving permanent stoma. After a median follow up of 21(3 to 60) months, postoperative complications were recorded in 12 patients (16.4%) with 16 episodes, including anastomotic stenosis (8.2%), rectum segmental stricture (5.5%), ileus (2.7%), partial anastomotic dehiscence (1.4%), anastomotic fistula (1.4%), rectovaginal fistula (1.4%) and mucosal prolapse (1.4%). These patients received corresponding treatments, such as endoscopic transanal resection, anal dilation, enema, purgative, permanent stoma, etc. according to the lesions. Six patients (8.2%) required re-operation intervention due to postoperative complications.

Conclusion: Anastomosis-associated morbidity is the most common after LapISR in the treatment of low rectal cancer in perioperative and postoperative periods, which must be strictly managed with suitable methods.
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April 2017

Identification and functional characterization of three type III polyketide synthases from Aquilaria sinensis calli.

Biochem Biophys Res Commun 2017 May 31;486(4):1040-1047. Epub 2017 Mar 31.

Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China. Electronic address:

Type III polyketide synthases (PKSs) play an important role in biosynthesis of various plant secondary metabolites and plant adaptation to environmental stresses. Aquilaria sinensis (A. sinensis) is the main plant species for production of agarwood, little is known about its PKS family. In this study, AsCHS1 and two new type III PKSs, AsPKS1 and AsPKS2, were isolated and characterized in A. sinensis calli. The comparative sequence and phylogenetic analysis indicated that AsPKS1 and AsPKS2 belonged to non-CHS group different from AsCHS1. The recombinant AsPKS1 and AsPKS2 produced the lactone-type products, suggesting their different enzyme activities from AsCHS1. Three PKS genes had a tissues-specific pattern in A. sinensis. Moreover, we examined the expression profiles of three PKS genes in calli under different abiotic stresses and hormone treatments. AsCHS1 transcript was most significantly induced by salt stress, AsPKS1 abundance was most remarkably enhanced by CdCl treatment, while AsPKS2 expression was most significantly induced by mannitol treatment. Furthermore, AsCHS1, AsPKS1 and AsPKS2 expression was enhanced upon gibberellins (GA3), methyl jasmonate (MeJA), or salicylic acid (SA) treatment, while three PKS genes displayed low transcript levels at the early stage under abscisic acid (ABA) treatment. In addition, three GFP:PKSs fusion proteins were localized in the cytoplasm and cell wall in Nicotiana benthamiana cells. These results indicated the multifunctional role of three type III PKSs in polyketide biosynthesis, plant resistance to abiotic stresses and signal transduction.
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http://dx.doi.org/10.1016/j.bbrc.2017.03.159DOI Listing
May 2017

[Risk factors of postoperative urinary retention after rectal cancer surgery].

Zhonghua Wei Chang Wai Ke Za Zhi 2017 Mar;20(3):295-299

Department of Colorectal Surgery, The General Hospital of The PLA Rocket Force, Beijing 100088, China.

Objective: To investigate the risk factors of postoperative urinary retention after rectal cancer surgery.

Methods: Clinical data of 133 patients with rectal cancer undergoing radical surgery from January 2013 to September 2014 in the General Hospital of the PLA Rocket Force were retrospectively analyzed. Time to the first removal of urinary catheter, incidence of postoperative urinary retention, and time to re-insert indwelling catheter were recorded. Risk factors of urinary retention were analyzed.

Results: Of 133 patients, 70 were males and 63 were females, with a median age of 62 (20-79) years old. Distance from tumor lower margin to anal verge were ≤5 cm in 58 patients, >5 cm to 10 cm in 41 patients, and >10 cm to 15 cm in 34 patients. The postoperative TNM stage was recorded in 35 patients with stage I(, 34 with stage II(, 59 with stage III( and 5 with stage IIII(. Surgical procedures included anterior resection (AR) for 92 patients, abdominoperineal resection (APR) for 25 patients and intersphincteric resection (ISR) for 16 patients. Laparoscopic approach was performed in 89 patients compared with open operation in 44 patients. Time to the first removal of urinary catheter was 2-7 days after operation (median, 5 days) and 36 (27.1%) patients developed urinary retention. All the 36 patients achieved spontaneous voiding by re-inserting urinary catheter for 2-28 days (median, 6 days). Univariate analysis showed that elderly (>65 years) and laparoscopic approach had significantly higher incidence of urinary retention [37.5%(21/56) vs. 19.5%(15/77), χ=5.333, P=0.021; 34.8%(31/89) vs. 11.4%(5/44), χ=8.214, P=0.004; respectively]. Multivariate logistic analysis demonstrated that old age(OR=3.949, 95%CI:1.622 to 9.612, P=0.002), laparoscopic approach (OR=5.665, 95%CI:1.908 to 16.822, P=0.002), and abdominoperineal resection (OR=3.443, 95%CI:1.199 to 9.887, P=0.022) were independent risk factors of urinary retention after rectal cancer surgery.

Conclusions: Patients undergoing rectal cancer surgery have a high risk of postoperative urinary retention. More attention should be paid to the old patients, especially those undergoing laparoscopic procedure or abdominoperineal resection, to prevent postoperative urinary retention and urinary dysfunction.
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March 2017

Doping of three-dimensional porous carbon nanotube-graphene-ionic liquid composite into polyaniline for the headspace solid-phase microextraction and gas chromatography determination of alcohols.

Anal Chim Acta 2016 Dec 11;948:48-54. Epub 2016 Nov 11.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China. Electronic address:

In this work, ionic liquid (IL, i.e. 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate), carboxyl multiwall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) were used to prepare three-dimensional porous material (MWCNTs-rGO-IL) by one-step self-assembly, then it was co-electrodeposited with polyaniline (PANI) on stainless steel wires by cyclic voltammetry. The resulting coating (PANI-MWCNTs-rGO-IL) was characterized by using FT-IR and scanning electron microscopy etc, and it showed porous structure and had high thermal stability. Furthermore, it was found to be very suitable for the headspace solid-phase microextraction of alcohols (i.e. octanol, nonanol, geraniol, decanol, undecanol and dodecanol). By coupling with gas chromatography, wide linear ranges and low limits of detection (i.e. 2.2-28.3 ng L) were obtained for the alcohols. The coating also presented good repeatability and reproducibility; the relative standard deviations for intra-fiber and fiber-to-fiber were less than 5.6% (n = 5) and 7.0% (n = 5) respectively. In addition, the proposed method was successfully applied to the determination of alcohols in tea drinks, and the recoveries for standards added were 85.6-114%.
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http://dx.doi.org/10.1016/j.aca.2016.11.020DOI Listing
December 2016

Mild hypothermia attenuate kidney injury in canines with oleic acid-induced acute respiratory distress syndrome.

Injury 2016 Jul 3;47(7):1445-51. Epub 2016 May 3.

Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, PR China. Electronic address:

Background: Hypothermia may attenuate ventilator induced-lung injury in acute respiratory distress syndrome (ARDS). However, the impact of hypothermia on extra-pulmonary organ injury in ARDS remains unclear. The purpose of this study was to investigate whether hypothermia affects extra-pulmonary organ injury in a canine ARDS model induced by oleic acid.

Objectives: Twelve anesthetized canines with oleic acid-induced ARDS were randomly divided (n=6 per group) into a hypothermia group (core temperature of 33±1°C, HT group) and a normothermia group (core temperature of 38±1°C, NT group) and treated for four hours. The liver, small intestine and kidney were assessed by evaluating biochemical parameters, plasma and tissue cytokine levels, and tissue histopathological injury scores.

Results: The HT group showed a lower plateau pressure, lung elastance and pulmonary vascular resistance. Hypothermia was associated with lower oxygen consumption (138.4±55.0mlmin(-1)vs. 72.0±11.2mlmin(-1), P<0.05) and higher oxygen saturation of mixed venous blood (62.8%±8.0% vs. 77.5%±10.1%, P<0.05). Both groups had similar levels of tumour necrosis factor-α in the plasma and extra-pulmonary organ, however, plasma interleukin-10 (97.1±25.0pgml(-1)vs. 131.4±27.0pgml(-1), P<0.05) was higher in the HT group. Further, the animals in the HT group had a lower levels of plasma creatinine (54.6±19.1UL(-1)vs. 29.1±8.0UL(-1), P<0.05), and lower renal histopathological injury scores [4.0(3.5;7.0) vs. 1.5(0.8;3.0), P<0.05]. Hypothermia did not affect the histopathological injury of the liver and small intestine.

Conclusions: Short-term mild hypothermia can reduce lung elastance and pulmonary vascular resistance, increase the systemic anti-inflammatory response and attenuate kidney histopathological injury in a canine ARDS model induced by oleic acid.
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http://dx.doi.org/10.1016/j.injury.2016.04.022DOI Listing
July 2016
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