Publications by authors named "Yingming Wang"

43 Publications

A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2.

Front Immunol 2021 22;12:653189. Epub 2021 Mar 22.

Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.

After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.653189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019923PMC
March 2021

Measuring the bias of technical change of industrial energy and environment productivity in China: a global DEA-Malmquist productivity approach.

Environ Sci Pollut Res Int 2021 Apr 1. Epub 2021 Apr 1.

Decision Sciences Institute, Fuzhou University, 2 Wulongjiang North Avenue, Fuzhou, People's Republic of China.

Thanks to the booming industry, China has made a huge economic achievement during the past several decades. However, it is suffering severe environmental and sustainable problems now. To find a sustainable development path, it is necessary to assess Chinese industrial energy and environment productivity and explore the contributing reasons. It is known that the technical change is the one power that drives the growth of the industrial productivity. Nevertheless, the technical change bias of Chinese industrial energy and environment productivity has rarely been analyzed, such that the secrets of Chinese industrial energy and environment productivity cannot be further uncovered. Thus, in this paper, we first propose a global DEA-Malmquist productivity index to evaluate the industrial energy and environment productivity of China and then figure out the Chinese industrial technical change biases by relaxing the Hicks' neutral assumption and decomposing the industrial technical change. We find out that both the global DEA-Malmquist productivity and the technical change are increased. Furthermore, the technical change drives the improvement of the global Malmquist productivity, but the technical progress is mainly driven by labor, energy consumption and CO emission biases. A multinomial logistic model is employed to find out the reasons for these biases. It finds that (1) the economic foundation has a significant positive impact on labor bias, while the infrastructures have negative impacts on labor bias. (2) CO emission bias is influence by energy prices positively. (3) The energy prices and the energy consumption structure have a negative influence on labor and energy bias, but the cost of curbing air pollutants and the size of the firm influence labor and energy bias positively. (4) The infrastructures and energy prices affect energy and CO emission bias positively, and the economic foundation and the size of the firm have negative impacts on energy and CO emission bias.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-13128-wDOI Listing
April 2021

Immune characteristics analysis reveals two key inflammatory factors correlated to the expressions of SARS-CoV-2 S1-specific antibodies.

Genes Dis 2020 Dec 23. Epub 2020 Dec 23.

Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.

The pandemic of COVID-19 caused by SARS-CoV-2 has made serious threats to the public health. Antibodies have been considered as promising therapeutics for the prevention and treatment of pathogens. So far, effectors that can influence the sustainability of SARS-CoV-2 specific antibodies in COVID-19 patients are still unclear. In this paper, we attempted to find potential key factors correlated with SARS-CoV-2 specific antibodies. Transcriptional analysis with the peripheral blood mononuclear cells (PBMCs) revealed proportional changes of immune cell subsets in COVID-19 convalescent patients, including a substantial decrease of monocytes and evident increase of dendritic cells (DCs). Moreover, we found that the gene expressions of chemokines associated with monocyte/macrophage were significantly up-regulated during the COVID-19 recovery phase. Most importantly, we found a set of 27 immune genes corresponding to a comparatively lower amount of SARS-CoV-2 specific antibodies, and identified two hub genes, and , the protein expressions of which exhibited negative correlation with the immunoglobulin G (IgG) levels in COVID-19 convalescent sera. In addition, we found that high expressions of these 2 hub genes during the convalescent stage were negatively associated with the plasma cell marker . Our study presented two key inflammatory factors correlated to the low level of SARS-CoV-2 specific antibodies, which indicated the potential regulatory process of plasmatic antibodies levels in some COVID-19 convalescent patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gendis.2020.12.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832135PMC
December 2020

Discovery of potent glycogen synthase kinase 3/cholinesterase inhibitors with neuroprotection as potential therapeutic agent for Alzheimer's disease.

Bioorg Med Chem 2021 Jan 11;30:115940. Epub 2020 Dec 11.

Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 211198, China. Electronic address:

In the present work, a novel series of pyridinethiazole bearing benzylpiperidine hybrids were designed and synthesized as dual-target inhibitors of GSK-3β/AChE. Among them, GD29 was the most promising candidate, with an IC value of 0.3 μM for hAChE and an IC value of 0.003 μM for hGSK-3β, respectively. The compounds exhibited good drug-like properties with optimal inhibitory enzyme activities. Moreover, GD29 showed anti-inflammatory properties at micromolar concentrations and displayed interesting neuroprotective profiles in an in vitro model of oxidative stress-induced neuronal death. Notably, the compounds also exhibited good permeability across the blood-brain-barrier (BBB) both in vitro. Central cholinomimetic activity was confirmed using a scopolamine-induced cognition impairment model in Institute of Cancer Research (ICR) mice upon oral administration. The current work identified optimized compounds and explored the therapeutic potential of glycogen synthase kinase 3/cholinesterase inhibition for the treatment of AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmc.2020.115940DOI Listing
January 2021

Improved photodynamic anticancer activity and mechanisms of a promising zinc(II) phthalocyanine-quinoline conjugate photosensitizer and .

Biomed Opt Express 2020 Jul 22;11(7):3900-3912. Epub 2020 Jun 22.

National and Local Joint Biomedical Engineering Research Center on Photodynamic Technologies, and Fujian Engineering Research Center for Drug and Diagnoses-Treat of Photodynamic Therapy, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.

Since the discovery of photodynamic therapy, scientists have constantly been searching for more effective and ideal photosensitizers (PSs). As part of our ongoing interest in the development of more potent photosensitizers, quinoline-8-yloxy-substituted zinc(II) phthalocyanine () has been identified as a promising photosensitizers in tumor cells. This study aims to explore the photodynamic mechanism and photodynamic efficacy of , and further evaluate its potential in clinical photodynamic therapy application. The single crystal structure of enables the easy control of clinical quality standards. In comparison with Photofrin, exhibits considerably higher anticancer activity by dual dose-related mechanisms (antiproliferative and apoptosis). In addition, the results demonstrate that exhibits significant tumor regression with less skin photosensitivity by both direct killing and apoptosis anticancer mechanisms. In conclusion, can be considered to be a promising ideal PS for clinical application owing to its defined chemical structure without phthalocyanine isomerization, good absorption of tissue-penetrating red light, improved photodynamic therapy efficacy, and reduced skin phototoxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/BOE.394186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510905PMC
July 2020

Microwave Hydrothermally Synthesized Metal-Organic Framework-5 Derived C-doped ZnO with Enhanced Photocatalytic Degradation of Rhodamine B.

Langmuir 2020 Aug 16;36(33):9658-9667. Epub 2020 Aug 16.

Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.

C-doped ZnO particles have been successfully prepared by the calcination using microwave hydrothermally prepared metal-organic framework-5 (MOF-5) as the precursor. MOF-5 was turned into C-doped ZnO through calcination at 500 °C, and its cubic shape was well-maintained. X-ray photoelectron spectroscopic studies confirmed the C-doping in the ZnO. The as-prepared C-doped ZnO demonstrated a Rhodamine B (RhB) degradation efficiency of 98% in 2 h under an solar-simulated light irradiation, much higher than that of C-doped ZnO derived from MOF-5 synthesized by the ordinary hydrothermal method. The trapping experiment revealed that the crucial factors in the RhB removal were photogenerated h and •O.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.0c00395DOI Listing
August 2020

Specific-oxygen-supply functionalized core-shell nanoparticles for smart mutual-promotion between photodynamic therapy and gambogic acid-induced chemotherapy.

Biomaterials 2020 10 10;257:120228. Epub 2020 Jul 10.

The Joint Laboratory of Chinese Pharmaceutical University and Taian City Central Hospital, Taian City Central Hospital, Taian, 271000, China; Tumor Precise Intervention and Translational Medicine Laboratory, Taian City Central Hospital, Taian, 271000, China. Electronic address:

Photodynamic therapy (PDT) and chemotherapy of cancer both meet respective challenges. Tumor hypoxia, low penetration and high glutathione (GSH) level bear the brunt. Herein, a core-shell nanoparticle, with multi-function of hypoxia-responsiveness, specific oxygen supply and deep tumor penetration, was constructed for smart mutual-promotion between the both to overcome the respective restrictions. The nano platform (GC@MCS NPs) was composed of hypoxia-responsive hyaluronic acid-nitroimidazole (HA-NI) as shells, MnO NPs as oxygen modulators and reduction-responsive functionalized poly (l-glutamic acid) derivatives (γ-PFGA) as cores to deliver gambogic acid (GA) and Chlorine6 (Ce6). After endocytosis, the approximately 100 nm of GC@MCS NPs achieved hypoxia-responsive shell degradation and MnO release, followed by reduction-activated charge conversion to form positively charged cores. With the damage effect of superficial tumor cells by the partially released GA, GA&Ce6-loadedγ-PFGA penetrated deep inside through electronic interaction step by step. Upon irradiated with 638 nm of laser, widely permeated Ce6 was activated for enhanced PDT under the high oxygenation by MnO NPs. The generated reactive oxygen species (ROS) in return facilitated the GA-induced paraptosis by clearing high level of GSH. As a result, this mutual promotion strategy contributed to 92.41% of 4T1 tumor inhibition rate, exhibiting outstanding advantages. Our GC@MCS NPs provided a smart combination of chemo-photodynamic therapy and focused on addressing the tumor hypoxia and low penetration issues.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2020.120228DOI Listing
October 2020

Improving the Antioxidation Capability of the Ni Catalyst by Carbon Shell Coating for Alkaline Hydrogen Oxidation Reaction.

ACS Appl Mater Interfaces 2020 Jul 1;12(28):31575-31581. Epub 2020 Jul 1.

College of Chemistry and Molecular Sciences, Hubei Key Lab of Electrochemical Power Sources, Wuhan University, Wuhan 430072, China.

Increasing the antioxidation capability of Ni for the hydrogen oxidation reaction (HOR) is considered important and challenging for alkaline polymer electrolyte fuel cells (APEFCs). Herein, we report a series of Ni-core carbon-shell (Ni@C) catalysts obtained by a vacuum pyrolysis method treated at different temperatures. According to the cyclic voltammetry tests and the HOR tests, Ni@C treated at 500 °C exhibits a much higher Ni core utilization and better catalytic activity toward HOR than the commonly used Ni/C catalyst. Furthermore, X-ray photoelectron spectroscopy characterization shows that a higher percentage of Ni appears at the surface of the Ni core of Ni@C than the Ni/C catalyst. The accelerated durability tests, as well as the chronoamperometry tests, suggest that the antioxidation capability of Ni has been obviously improved by the carbon shells. The Raman spectra show that the graphitization degree of the carbon shells might be the key factor affecting the Ni utilization and the HOR catalytic activity of the Ni@C catalysts. The APEFC achieves a peak power density of 160 mW/cm using Ni@C-500 °C as the anode, which could also stably discharge for 120 h at 0.7 V.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c10784DOI Listing
July 2020

Regional unified environmental efficiency of China: a non-separable hybrid measure under natural and managerial disposability.

Environ Sci Pollut Res Int 2020 Aug 11;27(22):27609-27625. Epub 2020 May 11.

School of Economics & Management, Fuzhou University, 2 Wulongjiang North Avenue, Fuzhou, People's Republic of China.

Attributing to the booming industry, China has made huge economic achievements during recent decades/years. However, the issue of energy and environment has challenged the sustainable development of the industry a lot in China. Investigating the non-separable relationship among energy, capital, and CO emission under natural and managerial disposability, this paper proposes two hybrid measure approaches to measure unified environmental efficiency of industry in China during 2011-2016. Besides, production efficiency, emission efficiency, damage to scale, and return to scale of 30 regions in China are calculated and recognized. The results show that (1) unified environmental efficiency of Chinese industry under natural disposability is higher than that under managerial disposability in early few years, but they are close to each other finally. (2) Unified environmental efficiency gaps among regions under natural disposability are wider than those under managerial disposability. Increasing capital investment and improving technology can help reduce efficiency gaps among regions. (3) It is available to increase production efficiency and reduce CO emission by cutting down energy consumption for most regions; insufficient capital investment and poor production technology cause the decreasing return to scale and production efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-09061-zDOI Listing
August 2020

Targeted degradation of anaplastic lymphoma kinase by gold nanoparticle-based multi-headed proteolysis targeting chimeras.

Colloids Surf B Biointerfaces 2020 Apr 13;188:110795. Epub 2020 Jan 13.

Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, Jiangsu 210009, China. Electronic address:

Anaplastic lymphoma kinase (ALK) is a major target in treating non-small-cell lung cancer, and several ALK inhibitors have been developed to antagonize its kinase activity. However, patients treated with inhibitors ultimately develop drug resistance. Therefore, therapies with new mechanisms of action are needed. Proteolysis targeting chimeras (PROTACs) are molecules that comprise a ligand for binding a protein of interest (POI), a connecting linker and a ligand for recruiting E3 ligase, and cause degradation of the target POI. Here, the first multi-headed PROTAC, as a proof of concept, is developed as a gold nanoparticle (GNP)-based drug delivery system for delivering PROTACs to target ALK. Pegylated GNPs loaded with both ceritinib and pomalidomide molecules, termed Cer/Pom-PEG@GNPs, showed good stability in several media. The GNP conjugates potently decreased the levels of ALK fusion proteins in a dose- and time-dependent manner, and specifically inhibited the proliferation of NCI-H2228 cells. In comparison with small molecule PROTACs, the new multi-headed PROTAC promoted the formation of coacervates of POIs/multi-headed PROTAC/E3 ubiquitin ligases, and POI and E3 ubiquitin ligase interacted through multidirectional ligands and a flexible linker, thereby avoiding the need for complicated structure optimization of PROTACs. In conclusion, Cer/Pom-PEG@GNPs can degrade intracellular ALK fusion proteins with minor off-target toxicity and can be applied in patients resistant to ALK inhibitors. As a nano-based drug carrier, Cer/Pom-PEG@GNPs have the potential to enable prolonged circulation and specifically distribute drugs to tumor regions in vivo; thus, further investigation is warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2020.110795DOI Listing
April 2020

An interprovincial evaluation of industrial energy and environment efficiency: what drives China to make progress in sustainability?

Environ Sci Pollut Res Int 2020 Feb 17;27(5):5222-5239. Epub 2019 Dec 17.

Decision Sciences Institute, Fuzhou University, Fuzhou, China.

China has made huge economic achievements based on industry. Heterogeneity, resulting from the industrial structure, energy endowment, and infrastructure among regions, has always been neglected on industrial performance evaluation. This paper focuses on provincial industrial heterogeneity to make a study. Firstly, meta-frontier slack-based measure is employed to rate industrial energy and environment efficiency of China. Besides, spatial evolution characteristics of efficiencies are described by global Moran's I and local Moran's I index. Taking the spatial correlation effect of efficiency into consideration, factors driving Chinese industrial energy and environment efficiency are analyzed and distinguished by quantitative regression and spatial Durbin model. It finds that (1) technical inefficiency and managerial inefficiency mainly attribute to the inefficiency of industrial energy and environment. (2) Chinese industrial energy and environment efficiency presents a stable spatial agglomeration as "high aggregation, low aggregation." It is necessary and indispensable for provinces and regions to cooperate to enhance efficiency. (3) Each province has a distinct driving mechanism of efficiency. The improvement policy of efficiency should be diversified for different provinces.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-019-07301-5DOI Listing
February 2020

A stage-specific cancer chemotherapy strategy through flexible combination of reduction-activated charge-conversional core-shell nanoparticles.

Theranostics 2019 21;9(22):6532-6549. Epub 2019 Aug 21.

Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.

Precision medicine has increased the demand for stage-specific cancer chemotherapy. Drugs with different properties are needed for different stages of tumor development, which is, inducing rapid destruction in the early stage and facilitating deep penetration in the advanced stage. Herein, we report a novel reduction-activated charge-conversional core-shell nanoparticle (CS NP) formula based on ring-closing metathesis of the thiamine disulfide system (TDS) to deliver the chemotherapeutic agent-gambogic acid (GA). The shell consisted of hyaluronic acid-all-trans retinoid acid with a disulfide bond as the linker (HA-SS-ATRA). The core was selected from poly (γ-glutamic acid) with different grafting rates of the functional group (Fx%) of TDS. GA/CS NPs, with the strongest reduction-responsive drug release, and GA/CS NPs with the strongest penetration have been finally screened. On this basis, a stage-specific administration strategy against a two-stage hepatocellular carcinoma was proposed. The developed CS NPs have been confirmed as inducing reduction-activated charge conversion from about -25 to +30 mV with up to 95% drug release within 48 h. The administration strategy, GA/CS NPs for the early-stage tumor, and sequential administration of GA/CS NPs followed by GA/CS NPs for the advanced-stage tumor, achieved excellent tumor inhibition rates of 93.86±2.94% and 90.76±6.43%, respectively. Our CS NPs provide a novel platform for charge conversion activated by reduction. The stage-specific administration strategy showed great promise for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.35057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771249PMC
October 2020

Two-Dimensional GaO/C Nanosheets as Durable and High-Rate Anode Material for Lithium Ion Batteries.

Langmuir 2019 Oct 8;35(42):13607-13613. Epub 2019 Oct 8.

The self-healing feature of gallium (Ga) is unique, making Ga-based materials attract attention for their potential to solve the anode pulverization issue of lithium ion batteries. In this work, a hierarchical two-dimensional (2D) GaO/C structure has been synthesized by a facile NaCl template method. GaO nanoparticles (3.8 nm) are uniformly embedded in 2D carbon nanosheets. The long horizontal length of the carbon nanosheets (10 μm) provides long-range electron conductivity, and the thin vertical thickness (75 nm) shortens the Li ion diffusion path. Benefited from the integrated 2D structure and the high electron conductivity, the obtained 2D GaO/C nanosheets exhibit excellent overall performance, including high lithium storage capacity (1026 mAh g at 0.5 A g), high rate capability (378 mAh g at 10.0 A g), and high cyclability (500 cycles at 0.5 A g). The lithiation/delithiation mechanism of 2D GaO/C has been further studied with combined electrochemical and X-ray diffraction methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.9b01826DOI Listing
October 2019

The Comparability of Pt to Pt-Ru in Catalyzing the Hydrogen Oxidation Reaction for Alkaline Polymer Electrolyte Fuel Cells Operated at 80 °C.

Angew Chem Int Ed Engl 2019 Jan 2;58(5):1442-1446. Epub 2019 Jan 2.

College of Chemistry and Molecular Sciences, Hubei Key Lab of Electrochemical Power Sources, Wuhan University, Wuhan, 430072, China.

The Pt-catalyzed hydrogen oxidation reaction (HOR) for alkaline polymer electrolyte fuel cells (APEFCs) has been one of the focus subjects in current fuel-cell research. The Pt catalyst is inferior for HOR in alkaline solutions, and alloying with Ru is an effective promotion strategy. APEFCs with Pt-Ru anodes have provided a performance benchmark over 1 W cm at 60 °C. The Pt anode is now found to be in fact as good as the Pt-Ru anode for APEFCs operated at elevated conditions. At 80 °C with appropriate gas back-pressure, the cell with a Pt anode exhibits a peak power density of about 1.9 W cm , which is very close to that with a Pt-Ru anode. Even by decreasing the anode Pt loading to 0.1 mg cm , over 1.5 W cm can still be achieved at 80 °C. This finding alters the previous understanding about the Pt catalyzed HOR in alkaline media and casts a new light on the development of practical and high-power APFEC technology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.201812662DOI Listing
January 2019

CysHis Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea.

Appl Environ Microbiol 2018 09 17;84(17). Epub 2018 Aug 17.

Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China

Abscisic acid (ABA) is one of the five classical phytohormones involved in increasing the tolerance of plants for various kinds of stresses caused by abiotic or biotic factors, and it also plays important roles in regulating the activation of innate immune cells and glucose homeostasis in mammals. For these reasons, as a "stress hormone," ABA has recently received attention as a candidate drug for agriculture and biomedical applications, prompting significant development of ABA synthesis. Some plant-pathogenic fungi can synthesize natural ABA. The fungus has been used for biotechnological production of ABA. Identification of the transcription factors (TFs) involved in regulation of ABA biosynthesis in would provide new clues to understand how ABA is synthesized and regulated. In this study, we defined a novel CysHis TF, BcabaR1, that regulates the transcriptional levels of ABA synthase genes (, , , and ) in an ABA-overproducing mutant, TBC-A. Electrophoretic mobility shift assays revealed that recombinant BcabaR1 can bind specifically to both a 14-nucleotide sequence motif and a 39-nucleotide sequence motif in the promoter region of to - genes A decreased transcriptional level of the gene in led to significantly decreased ABA production and downregulated transcription of to - When was overexpressed in , ABA production was significantly increased, with upregulated transcription of to - Thus, in this study, we found that BcabaR1 acts as a positive regulator of ABA biosynthesis in Abscisic acid (ABA) could make a potentially important contribution to theoretical research and applications in agriculture and medicine. is a plant-pathogenic fungus that was found to produce ABA. There has been a view that ABA is related to the interaction between pathogenic fungi and plants. Identification of regulatory genes involved in ABA biosynthesis may facilitate an understanding of the underlying molecular mechanisms of ABA biosynthesis and the pathogenesis of Here, we present a positive regulator, BcabaR1, of ABA biosynthesis in that can affect the transcriptional level of the ABA biosynthesis gene cluster, to -, by directly binding to the conserved sequence elements in the promoter of the to - genes. This TF was found to be specifically involved in regulation of ABA biosynthesis. This work provides new clues for finding other ABA biosynthesis genes and improving ABA yield in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/AEM.00920-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102986PMC
September 2018

Effect of arthroscopic resection for discoid lateral meniscus on the axial alignment of the lower limb.

Int Orthop 2018 08 16;42(8):1897-1903. Epub 2018 May 16.

Orthopedic Department, Anhui Provincial Hospital, Anhui Medical University, Hefei, 230001, China.

Purpose: This study was designed to investigate the changes in the lower limb axial alignment and knee joint function after arthroscopic resection of discoid lateral menisci.

Methods: Pre-operative and post-operative full-length weight-bearing radiographs of the lower limb were obtained from 60 patients with discoid lateral menisci from August 2015 to August 2016. Twenty-four patients were treated with meniscectomy and 36 cases were treated with meniscoplasty. The axial alignment of the lower limb was measured, and changes in the lower limb axial alignment before and after surgery were analyzed. The effects of differing degrees of meniscal resection on the lower limb axial alignment were compared and analyzed. Knee joint function on the affected side was scored using the Lysholm knee scoring, Tegner activity, and International Knee Documentation Committee (IKDC) subjective scales before surgery and one, six and 12 months after surgery.

Results: There were apparent changes in the lower limb axial alignment after surgery (p < 0.01). The changes were more conspicuous after a total meniscectomy than a meniscoplasty but were insignificant (p > 0.05). Intragroup comparisons of the Lysholm knee, IKDC, and Tegner scores before and after surgery revealed significant differences (p < 001). However, the differences were not significant between the two surgical approaches (p > 0.05).

Conclusion: For those with considerable genu varum or genu valgum after surgery, individualized therapy should be developed to correct the lower limb axial alignment and to prevent articular cartilage degeneration. Arthroscopic resection of a discoid lateral meniscus greatly improves knee joint function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00264-018-3944-5DOI Listing
August 2018

Robot-assisted versus conventional laparoscopic operation in anus-preserving rectal cancer: a meta-analysis.

Ther Clin Risk Manag 2017 22;13:1247-1257. Epub 2017 Sep 22.

Department of Gastrointestinal Cancer Surgery, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences.

Objective: The aim of this meta-analysis is to provide recommendations for clinical practice and prevention of postoperative complications, such as circumferential resection margin (CRM) involvement, and compare the amount of intraoperative bleeding, safety, operative time, recovery, outcomes, and clinical significance of robot-assisted and conventional laparoscopic procedures in anus-preserving rectal cancer.

Methods: A literature search (PubMed) was performed to identify biomedical research papers and abstracts of studies comparing robot-assisted and conventional laparoscopic procedures. We attempted to obtain the full-text link for papers published between 2000 and 2016, and hand-searched references for relevant literature. RevMan 5.3 software was used for the meta-analysis.

Results: Nine papers (949 patients) were eligible for inclusion; there were 473 patients (49.8%) in the robotic group and 476 patients (50.2%) in the laparoscopic group. According to the data provided in the literature, seven indicators were used to complete the evaluation. The results of the meta-analysis suggested that robot-assisted procedure was associated with lower intraoperative blood loss (mean difference [MD] -41.15; 95% confidence interval [CI] -77.51, -4.79; =0.03), lower open conversion rate (risk difference [RD] -0.05; 95% CI -0.09, -0.01; =0.02), lower hospital stay (MD -1.07; 95% CI -1.80, -0.33; =0.005), lower overall complication rate (odds ratio 0.58; 95% CI 0.41, 0.83; =0.003), and longer operative time (MD 33.73; 95% CI 8.48, 58.99; =0.009) compared with conventional laparoscopy. There were no differences in the rate of CRM involvement (RD -0.02; 95% CI -0.05, 0.01; =0.23) and days to return of bowel function (MD -0.03; 95% CI -0.40, 0.34; =0.89).

Conclusion: The Da Vinci robot was superior to laparoscopy with respect to blood loss, open conversion, hospital stay, and postoperative complications during anus-preserving rectal cancer procedures; however, conventional laparoscopy had an advantage regarding operative time. The remaining indicators (CRMs and recovery from intestinal peristalsis) did not differ.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/TCRM.S142758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626418PMC
September 2017

RHEB1 insufficiency in aged male mice is associated with stress-induced seizures.

Geroscience 2017 12 10;39(5-6):557-570. Epub 2017 Sep 10.

OHSU Transgenic Mouse Models Shared Resource, Knight Cancer Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR, 97239, USA.

The mechanistic target of rapamycin (mTOR), a protein kinase, is a central regulator of mammalian metabolism and physiology. Protein mTOR complex 1 (mTORC1) functions as a major sensor for the nutrient, energy, and redox state of a cell and is activated by ras homolog enriched in brain (RHEB1), a GTP-binding protein. Increased activation of mTORC1 pathway has been associated with developmental abnormalities, certain form of epilepsy (tuberous sclerosis), and cancer. Clinically, those mTOR-related disorders are treated with the mTOR inhibitor rapamycin and its rapalogs. Because the effects of chronic interference with mTOR signaling in the aged brain are yet unknown, we used a genetic strategy to interfere with mTORC1 signaling selectively by introducing mutations of Rheb1 into the mouse. We created conventional knockout (Rheb1 ) and gene trap (Rheb1 ) mutant mouse lines. Rheb1-insufficient mice with different combinations of mutant alleles were monitored over a time span of 2 years. The mice did not show any behavioral/neurological changes during the first 18 months of age. However, after aging (> 18 months of age), both the Rheb1 and Rheb1 hybrid males developed rare stress-induced seizures, whereas Rheb1 and Rheb1 females and Rheb1 and Rheb1 mice of both genders did not show any abnormality. Our findings suggest that chronic intervention with mTORC1 signaling in the aged brain might be associated with major adverse events.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11357-017-9997-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745219PMC
December 2017

Tuning the Morphology of LiO by Noble and 3d metals: A Planar Model Electrode Study for Li-O Battery.

ACS Appl Mater Interfaces 2017 Jun 5;9(23):19800-19806. Epub 2017 Jun 5.

College of Chemistry and Molecular Sciences, Hubei Key Lab of Electrochemical Power Sources, Wuhan University , Wuhan 430072, China.

In this work, a planar model electrode method has been used to investigate the structure-activity relationship of multiple noble and 3d metal catalysts for the cathode reaction of Li-O battery. The result shows that the battery performance (discharge/charge overpotential) strongly depends not only on the type of catalysts but also on the morphology of the discharge product (LiO). Specifically, according to electrochemical characterization and scanning electron microscopy (SEM) observation, noble metals (Pd, Pt, Ru, Ir, and Au) show excellent battery performance (smaller discharge/charge overpotential), with wormlike LiO particles with size less than 200 nm on their surfaces. On the other hand, 3d metals (Fe, Co, Ni, and Mn) offered poor battery performance (larger discharge/charge overpotential), with much larger LiO particles (1 μm to a few microns) on their surfaces after discharging. Further research shows that a "volcano plot" is found by correlating the discharging/charging plateau voltage with the adsorption energy of LiO on different metals. The metals with better battery performance and worm-like-shaped LiO are closer to the top of the "volcano", indicating adsorption energy of LiO is one of the key characters for the catalyst to reach a good performance for the oxygen electrode of Li-O battery, and it has a strong influence on the morphology of the discharge product on the electrode surface.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.7b02663DOI Listing
June 2017

Relationship Between Dynamic Changes in Expression of IL-17/IL-23 in Lacrimal Gland and Ocular Surface Lesions in Ovariectomized Mice.

Eye Contact Lens 2018 Jan;44(1):35-43

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Purpose: An ovariectomized mouse model was constructed to observe the dynamic effects of hormone changes on the expression of interleukin (IL)-17A and IL-23 in the lacrimal glands.

Methods: The ovariectomized mouse model was constructed by bilateral ovary removal. The concentrations of serum estradiol and testosterone in mouse cardiac blood were detected by radioimmunoassay. Mice in both groups underwent the phenol red cotton thread test and corneal fluorescein staining to assess the ocular surface, whereas Th17 cells in blood and spleen were detected by flow cytometry. IL-17A and IL-23 expression in the lacrimal glands was detected by immunohistochemistry, enzyme-linked immunosorbent assay, and real-time fluorescence quantitative polymerase chain reaction.

Results: Serum estradiol and testosterone levels were significantly lower in the ovariectomized group compared with those in the control group. There was lymphocytic infiltration in the lacrimal gland of the ovariectomized group mice. At 6 months after the surgery, aqueous tear production was significantly lower, and statistically significant corneal fluorescein staining was found in the ovariectomized group, compared with that in the control group. In the ovariectomized group, IL-17A and the IL-23 expression in the lacrimal glands and the Th17 expression in the blood and spleen were significantly higher than in the control group.

Conclusion: The hormone levels are significantly reduced and lymphocytic infiltration in the lacrimal gland in ovariectomized mice, whereas the frequency of Th17 cells in the blood and spleen and IL-17A and IL-23 expression in the lacrimal glands are increased, leading to reduced tear production and positive fluorescein staining in the cornea.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/ICL.0000000000000289DOI Listing
January 2018

Self-assembled triangular DNA nanoparticles are an efficient system for gene delivery.

J Control Release 2016 07 15;233:126-35. Epub 2016 May 15.

Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address:

Developing an advanced nucleic acid drug delivery system is of great significance in order to achieve optimal gene delivery. Self-assembled nucleic acid nanoparticles are an excellent platform for the delivery of nucleic acids and other small molecular drugs. In this study, we developed the efficient, three-stranded, RNA/DNA hybrid triangular self-assembled nanoparticles, namely, mTOR single-stranded siRNA-loaded triangular DNA nanoparticles (ssRNA-TNP). The ssRNA-TNP is formed by the complementary association of the above mentioned three components and is more stable in complete medium than standard duplex siRNA. It could be efficiently transfected into NCI-H292 cells in a dose- and time-dependent manner, resulting in high transfection efficiency. Furthermore, ssRNA-TNP uptake is dependent on macropinocytosis and clathrin-mediated endocytosis pathways. Interestingly, ssRNA-TNP is more efficient to inhibit the expression of mTOR. This ssRNA-TNP has a simpler structure, better stability, and higher transfection efficiency; therefore it may become a novel nonviral nanosystem for gene delivery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2016.05.038DOI Listing
July 2016

RHEB1 expression in embryonic and postnatal mouse.

Histochem Cell Biol 2016 May 26;145(5):561-72. Epub 2015 Dec 26.

OHSU Transgenic Mouse Models Shared Resource, Knight Cancer Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR, 97239, USA.

Ras homolog enriched in brain (RHEB1) is a member within the superfamily of GTP-binding proteins encoded by the RAS oncogenes. RHEB1 is located at the crossroad of several important pathways including the insulin-signaling pathways and thus plays an important role in different physiological processes. To understand better the physiological relevance of RHEB1 protein, the expression pattern of RHEB1 was analyzed in both embryonic (at E3.5-E16.5) and adult (1-month old) mice. RHEB1 immunostaining and X-gal staining were used for wild-type and Rheb1 gene trap mutant mice, respectively. These independent methods revealed similar RHEB1 expression patterns during both embryonic and postnatal developments. Ubiquitous uniform RHEB1/β-gal and/or RHEB1 expression was seen in preimplantation embryos at E3.5 and postimplantation embryos up to E12.5. Between stages E13.5 and E16.5, RHEB1 expression levels became complex: In particular, strong expression was identified in neural tissues, including the neuroepithelial layer of the mesencephalon, telencephalon, and neural tube of CNS and dorsal root ganglia. In addition, strong expression was seen in certain peripheral tissues including heart, intestine, muscle, and urinary bladder. Postnatal mice have broad spatial RHEB1 expression in different regions of the cerebral cortex, subcortical regions (including hippocampus), olfactory bulb, medulla oblongata, and cerebellum (particularly in Purkinje cells). Significant RHEB1 expression was also viewed in internal organs including the heart, intestine, urinary bladder, and muscle. Moreover, adult animals have complex tissue- and organ-specific RHEB1 expression patterns with different intensities observed throughout postnatal development. Its expression level is in general comparable in CNS and other organs of mouse. Thus, the expression pattern of RHEB1 suggests that it likely plays a ubiquitous role in the development of the early embryo with more tissue-specific roles in later development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00418-015-1394-3DOI Listing
May 2016

A PtRu catalyzed rechargeable oxygen electrode for Li-O2 batteries: performance improvement through Li2O2 morphology control.

Phys Chem Chem Phys 2014 Oct 26;16(38):20618-23. Epub 2014 Aug 26.

College of Chemistry and Molecular Sciences, Hubei Key Lab of Electrochemical Power Sources, Wuhan University, Wuhan 430072, China.

Albeit ultrahigh in energy density, the Li-O2 battery technology still suffers from the high overpotential of Li2O2 oxidation upon charging and the low cyclability. In the present work, we use Pt2Ru/C as the oxygen-electrode catalyst and study how it improves the cell performance and changes the reaction mechanism, as compared with a carbon electrode. Multiple methods, including X-ray diffraction, transmission/scanning electron microscopy, Raman spectroscopy, and cyclic voltammetry, have been employed for material characterization and reaction monitoring. The Li-O2 cell with a Pt2Ru/C catalyst shows lower charge voltage, higher specific capacity, and enhanced cyclability than does a carbon catalyst. The key for this improvement is ascribed to the morphology change of Li2O2. Whereas the Li2O2 formed in the carbon electrode is rod-shaped, the Li2O2 in the Pt2Ru/C electrode is mud shaped and closely attached to the electrode substrate, thus benefiting the subsequent Li2O2 oxidation. This study indicates that the charging performance of the Li-O2 battery can be improved not only by using proper catalysts, but also by controlling the Li2O2 morphology during discharge.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c4cp02646bDOI Listing
October 2014

Plasma surface modification of rigid contact lenses decreases bacterial adhesion.

Eye Contact Lens 2013 Nov;39(6):376-80

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objective: Contact lens safety is an important topic in clinical studies. Corneal infections usually occur because of the use of bacteria-carrying contact lenses. The current study investigated the impact of plasma surface modification on bacterial adherence to rigid contact lenses made of fluorosilicone acrylate materials.

Methods: Boston XO and XO2 contact lenses were modified using plasma technology (XO-P and XO2-P groups). Untreated lenses were used as controls. Plasma-treated and control lenses were incubated in solutions containing Staphylococcus aureus or Pseudomonas aeruginosa. MTT colorimetry, colony-forming unit counting method, and scanning electron microscopy were used to measure bacterial adhesion.

Results: MTT colorimetry measurements showed that the optical density (OD) values of XO-P and XO2-P were significantly lower than those of XO and XO2, respectively, after incubation with S. aureus (P < 0.01). The OD value of XO-P was also much lower than that of XO after incubation with P. aeruginosa (P < 0.01). Colony-forming unit counting revealed that a significantly lower number of bacterial colonies attached to the XO-P versus XO lenses and to the XO2-P versus XO2 lenses incubated with S. aureus (P < 0.01). Fewer bacterial colonies attached to the XO-P versus XO lenses incubated with P. aeruginosa (P < 0.01). Further, scanning electron microscopy suggested different bacterial adhesion morphology on plasma-treated versus control lenses.

Conclusion: Plasma surface modification can significantly decrease bacterial adhesion to fluorosilicone acrylate contact lenses. This study provides important evidence of a unique benefit of plasma technology in contact lens surface modification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/ICL.0b013e31829e8f73DOI Listing
November 2013

Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa.

Protein Expr Purif 2013 Dec 11;92(2):208-13. Epub 2013 Oct 11.

R&D Department, Novoprotein Scientific Inc (Shanghai), Room 202, Building 2, 720 Cailun Road, Shanghai 201203, China; State Key Laboratory of Genetic Engineering and MOE, Key Laboratory of Contemporary Anthropology, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.

As a member of zinc-containing medium-chain alcohol dehydrogenase family, formaldehyde dehydrogenase (FDH) can oxidize toxic formaldehyde to less active formate with NAD(+) as a cofactor and exists in both prokaryotes and eukaryotes. Most FDHs are well known to be glutathione-dependent in the catalysis of formaldehyde oxidation, but the enzyme from Pseudomonas putida is an exception, which is independent of glutathione. To identify novel glutathione-independent FDHs from other bacterial strains and facilitate the corresponding structural and enzymatic studies, high-level soluble expression and efficient purification of these enzymes need to be achieved. Here, we present molecular cloning, expression, and purification of the FDH from Pseudomonas aeruginosa, which is a Gram-negative pathogenic bacterium causing opportunistic human infection. The FDH of P. aeruginosa shows high sequence identity (87.97%) with that of P. putida. Our results indicated that coexpression with molecular chaperones GroES, GroEL, and Tig has significantly attenuated inclusion body formation and improved the solubility of the recombinant FDH in Escherichiacoli cells. A purification protocol including three chromatographic steps was also established to isolate the recombinant FDH to homogeneity with a yield of ∼3.2 mg from 1L of cell culture. The recombinant P. aeruginosa FDH was properly folded and biologically functional, as demonstrated by the mass spectrometric, crystallographic, and enzymatic characterizations of the purified proteins.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pep.2013.09.017DOI Listing
December 2013

Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Sep 19;69(Pt 9):967-72. Epub 2013 Aug 19.

R&D Department, Novoprotein Scientific Inc. (Shanghai), R202, Building 2, 720 Cailun Road, Shanghai 201203, People's Republic of China.

Formaldehyde dehydrogenase (FDH) is a member of the zinc-containing medium-chain alcohol dehydrogenase family which oxidizes toxic formaldehyde to formate using NAD(+) as an electron carrier. Three-dimensional structures have been reported for FDHs from several different species. Most FDHs are dependent on glutathione for catalysis, but the enzyme from Pseudomonas putida is an exception. In this structural communication, the recombinant production, crystallization and X-ray structure determination at 2.7 Å resolution of FDH from P. aeruginosa are described. Both the tetrameric assembly and the NAD(+)-binding mode of P. aeruginosa FDH are similar to those of P. putida FDH, which is in good agreement with the high sequence identity (87.97%) between these two proteins. Preliminary enzymatic kinetics studies of P. aeruginosa FDH also revealed a conserved glutathione-independent `ping-pong' mechanism of formaldehyde oxidization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1107/S174430911302160XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758142PMC
September 2013

Cloning and characterization of a novel human BRMS1 transcript variant in hepatocellular carcinoma cells.

Cancer Lett 2013 Sep 30;337(2):266-75. Epub 2013 Apr 30.

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.

Breast cancer metastasis suppressor 1 (BRMS1) is able to suppress tumor metastasis without affecting primary tumor growth in various cancers. Here, we report a novel transcript variant of human BRMS1, termed BRMS1.vh. BRMS1.vh is identical to the major BRMS1 variant (BRMS1.v1) except for missing base pairs 683-775, encoding a 215-amino acid protein lacking a functional nuclear localization sequence. Expression of BRMS1.vh in hepatocellular carcinoma (HCC) cells suppressed NF-κB signaling pathway, sensitized cells to apoptotic stimuli, leading to suppressed tumor growth. Taken together, our results suggest a potential role for BRMS1.vh in regulating cell apoptosis and tumor growth in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2013.04.030DOI Listing
September 2013

ADAM23 knockdown promotes neuronal differentiation of P19 embryonal carcinoma cells by up-regulating P27KIP1 expression.

Cell Biol Int 2012 ;36(12):1275-9

School of Life Sciences, Fudan University, Shanghai, People's Republic of China.

ADAM23 (a disintegrin and metalloprotease 23), a member of brain MDC (macrophage-derived chemokine) family, is important for the development of CNS (central nervous system). P19 mouse embryonal carcinoma cells can differentiate into neurons when cultured in aggregates and induced with RA (retinoic acid). We have found that under conditions without RA induction, knocking down ADAM23 with RNAi (RNA interference) promoted neuronal differentiation, and similarly recombinant GST (glutathione transferase)-ADAM23-DIS protein inhibited neuronal differentiation of P19/ADAM23KD (P19/ADAM23-knockdown) cells. In P19/ADAM23KD, there were more cells arrested in G1 phase than normal P19 cells, due to the up-regulation of P57KIP2 and P27KIP1 expression. P27KIP1 was up-regulated during the differentiation process of both P19/ADAM23KD cells without RA induction, and P19 cells with RA induction. Transient overexpression of P27KIP1 in P19 cells also promoted neuronal differentiation of P19 cells. The findings indicate that ADAM23 suppresses neuronal differentiation through its disintegrin domain, and Adam23 KD up-regulates P27KIP1 in P19/ADAM23KD cells, one reason that P19/ADAM23KD cells can differentiate into neurons without RA induction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/CBI20120154DOI Listing
April 2013

Breast cancer metastasis suppressor 1 regulates hepatocellular carcinoma cell apoptosis via suppressing osteopontin expression.

PLoS One 2012 21;7(8):e42976. Epub 2012 Aug 21.

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as an active metastasis suppressor in human breast cancer. Loss of BRMS1 expression correlates with tumor progression, and BRMS1 suppresses several steps required for tumor metastasis. However, the role of BRMS1 in hepatocellular carcinoma (HCC) remains elusive. In this study, we found that the expression level of BRMS1 was significantly down-regulated in HCC tissues. Expression of BRMS1 in SK-Hep1 cells did not affect cell growth under normal culture conditions, but sensitized cells to apoptosis induced by serum deprivation or anoikis. Consistently, knockdown of endogenous BRMS1 expression in Hep3B cells suppressed cell apoptosis. We identified that BRMS1 suppresses osteopontin (OPN) expression in HCC cells and that there is a negative correlation between BRMS1 and OPN mRNA expression in HCC tissues. Moreover, knockdown of endogenous OPN expression reversed the anti-apoptosis effect achieved by knockdown of BRMS1. Taken together, our results show that BRMS1 sensitizes HCC cells to apoptosis through suppressing OPN expression, suggesting a potential role of BRMS1 in regulating HCC apoptosis and metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042976PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424258PMC
January 2013

Dual effects of sodium butyrate on hepatocellular carcinoma cells.

Mol Biol Rep 2012 May 7;39(5):6235-42. Epub 2012 Jan 7.

State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, 220 Handan Road, Shanghai, 200433, People's Republic of China.

Sodium butyrate (NaBu), a histone deacetylase inhibitor, has been shown to inhibit cell growth, induce cell differentiation and apoptosis in multiple cell lines. In present study, we revealed the dual effects of NaBu in regulating hepatocellular carcinoma (HCC) cells. In two different HCC cell lines, SK-Hep1 and SMMC-7721, low concentrations of NaBu induced a significant increase in cell growth ratio and S-phase cell percentage, accompanied by a reduced p21 Cip1 expression at both mRNA and protein levels, while dissimilarly, high concentrations of NaBu inhibited cell growth and induced G1 arrest through up-regulation of p21 Cip1 and p27 Kip1 protein expression. The reduction of p45 Skp2 expression further indicated that the ubiquitin-mediated protein degradation might play a role in NaBu-induced up-regulation of p21 Cip1 and p27 Kip1. Moreover, the high concentration of NaBu was also able to trigger HCC cell apoptosis. Taken together, these results demonstrate the distinct effects of NaBu at different dosages. This finding may contribute to develop more effective tumor therapeutic protocols of NaBu in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-011-1443-5DOI Listing
May 2012