Publications by authors named "Yinglong Huang"

13 Publications

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A Novel Long Non-Coding RNA KMU15 Promotes Growth and Chemoresistance of Bladder Cancer.

Clin Lab 2019 Dec;65(12)

Background: Chemotherapy constitutes one of the most important adjuvant treatments for bladder cancer. However, many patients usually develop chemoresistance during chemotherapy. At present, lncRNA has been confirmed not only to be involved in tumorigenesis and progression, but also in tumor chemoresistance. However, the relationship between lncRNAs and chemoresistance of bladder cancer have been rarely reported.

Methods: The novel lncRNA-KMU15 was screened by lncRNAs microarray and determination of IC50 in bladder cancer. The expression of KMU15 was evaluated by qRT-PCR. The correlation between KMU15 and clinicopathological parameters was analyzed from clinical cases. The effects of KMU15 on the biological behavior and chemoresistance were investigated by [3H]-TdR incorporation assay and other experiments. The effects of KMU15 on the growth of xenograft tumors and the survival of nude mice under cisplatin were examined in a xenograft mouse model.

Results: We confirmed that KMU15 was expressed higher in bladder cancer tissues than paired control tissues. Moreover, the expression of KMU15 was significantly positively correlated with the grade, stage, metastasis, and recurrence of bladder cancer and was significantly negatively correlated with the prognosis. In addition, KMU15 knockdown could significantly inhibit bladder cell proliferation, adhesion, migration, and chemoresistance and promoted apoptosis. Knockdown of KMU15 inhibited the growth of xenografts in nude mice and significantly prolonged the survival of tumor-bearing mice under cisplatin.

Conclusions: The novel lncRNA KMU15, which is highly expressed in bladder cancer tissues, could promote the proliferation and progression and was closely related to the malignant degree of bladder cancer. It could also significantly enhance the chemoresistance of bladder cancer cells. Therefore, it was expected to be a new therapy target for bladder cancer and a potential prognosis biomarker for chemotherapy.
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http://dx.doi.org/10.7754/Clin.Lab.2019.190532DOI Listing
December 2019

Association of rs8444 polymorphism in the LASS2 3'-UTR and bladder cancer risk in Chinese population.

Eur J Cancer Prev 2020 07;29(4):329-337

Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Yunnan Institute of Urology.

The aim of the present study was to explore the correlations between single nucleotide polymorphisms in LASS2 gene 3'-untranslated regions and bladder cancer risk in Chinese population. We first performed PCR and sequence for LASS2-3'-UTR in 105 bladder cancer patients and 100 control subjects. Next, multivariate logistic regression analysis was used to determine the relationship between single nucleotide polymorphisms frequency and susceptibility of bladder cancer, and clinical features in 105 cases. In addition, survival curves and Cox Regression analysis were used to investigate the effect of single nucleotide polymorphisms on clinical outcome in 58 cases. Finally, quantitative reverse-transcription PCR and immunohistochemical were performed to explore the influence of single nucleotide polymorphisms on LASS2 expression. We found that a single nucleotide polymorphism (rs8444 C>T) located in the 3'-UTR of LASS2 was significantly associated with the risk of bladder cancer. We also showed the frequency of rs8444 T genotype was higher in bladder cancer group and correlated with the risk of clinical prognosis. Yet, there were no significant correlations between T/C allele frequencies and the distributions of rs8444 genotype and tumor-node-metastasis stage, histological grade and distant metastasis in bladder cancer. Furthermore, we demonstrated that rs8444 C>T could affect LASS2 expression by single nucleotide polymorphism-related mRNA stability. Our results showed that LASS2-3'-UTR rs8444 C>T polymorphism was significantly associated with the individual risk and the poor overall survival of bladder cancer, suggesting that rs8444 TT genotype maybe act as an independent risk factor of susceptibility and clinical prognosis for bladder cancer in Chinese population.
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http://dx.doi.org/10.1097/CEJ.0000000000000551DOI Listing
July 2020

miR-3622a promotes proliferation and invasion of bladder cancer cells by downregulating LASS2.

Gene 2019 Jun 19;701:23-31. Epub 2019 Mar 19.

Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Yunnan Institute of Urology, Kunming 650101, China. Electronic address:

As a tumor metastasis suppressor gene, LASS2 has been found to be negatively associated with the stage of bladder cancer and overall survival of patients. However, the mechanisms regulating LASS2 in bladder cancer remain poorly understood. Here, we aim to identify a miRNA that targets LASS2 from bladder cancer-associated miRNAs and to reveal its potential functions in bladder cancer cells. Through miRNA microarray and bioinformatics analyses, we identified miR-3622a as a negative regulator of LASS2. The expression levels of miR-3622a in bladder cancer tissues were negatively correlated with the overall survival of patients. Overexpression of miR-3622a significantly increased the proliferation and invasion abilities of bladder cancer cells. In conclusion, our results indicate that miR-3622a promotes the proliferation and invasion of bladder cancer cells by downregulating LASS2.
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http://dx.doi.org/10.1016/j.gene.2019.02.083DOI Listing
June 2019

A novel mutation of SGSH and clinical features analysis of mucopolysaccharidosis type IIIA.

Medicine (Baltimore) 2018 Dec;97(52):e13758

Department of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, P.R. China.

Rationale: The aim of this study was to analyze the clinical and imaging features of a pediatric patient with mucopolysaccharidosis type IIIA (MPS IIIA) and a novel mutation of the N-sulfoglucosamine sulfohydrolase (SGSH) in 1 pedigree.

Patient Concerns: An 8-year-old female patient presented with developmental regression, seizures, cerebral atrophy, thickened calvarial diploe, apathy, esotropia, slender build, thick hair, prominent eyebrows, hepatomegaly, ankle clonus, muscle and joint contractures, and funnel chest.

Diagnoses: The patient was diagnosed as autosomal recessive (AR) MPS IIIA with a novel mutation in the SGSH gene.

Interventions: Genomic DNA was extracted from the peripheral blood and next-generation sequencing (NGS) technology was used to detect pathogenic genes, and the Sanger method was applied to perform pedigree verification for the detected suspicious pathogenic mutations.

Outcomes: The NGS done for the girl and her family showed 2 variations that were both missense mutations in SGSH. The c.1298G > A (p.Arg433Gln) was a known mutation, and the c.630 G > T (p.Trp210Cys) was a novel variation.

Lessons: The common clinical manifestations of MPS IIIA were rapid developmental regression, seizures, cerebral atrophy, and thickened calvarial diploe. The results showed that the c.630 G > T was likely pathogenic according to bioinformatics analysis, which probably was a novel mutation. This study reports 1 case of MPS IIIA with some clinical features as determined via clinical and genetic analysis, and found a new mutation in the SGSH gene.
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http://dx.doi.org/10.1097/MD.0000000000013758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314651PMC
December 2018

Hsa-miR-3658 Promotes Cell Proliferation, Migration and Invasion by Effecting LASS2 in Bladder Cancer.

Clin Lab 2018 Apr;64(4):515-525

Background: Hsa-miR-3658 is upregulated in various tumors, but its expression in bladder cancer has been rarely studied.

Methods: In this study, hsa-miR-3658 expressions in several bladder cancer cell lines were examined, and its effect on the malignant degree of bladder cancer and whether hsa-miR-3658 regulates tumor biological behaviors through LASS2 and its downstream molecular pathway were studied and validated.

Results: It was found miR-3658 expressions differed among different cell lines, which may be an influence factor on the malignancy. MiR-3658 can enhance the proliferation, migration and invasion of bladder cancer cells, inhibit cell adhesion and reduce cell chemosensitivity. MiR-3658 can promote the epithelial-mesenchymal transition of bladder cancer cells through the molecular mechanism of affecting the expressions of epithelial-mesenchymal transition marker protein and related transcription factors.

Conclusions: MiRNA-3658 is upregulated in bladder cancer cells, and this change is associated with the proliferation, invasion and resistance of bladder cancer cells. The effect of miR-3658 on bladder cancer cell biology may be associated with the effect on LASS2.
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http://dx.doi.org/10.7754/Clin.Lab.2017.171026DOI Listing
April 2018

LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells.

J Cancer 2018 28;9(6):1017-1024. Epub 2018 Feb 28.

Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Yunnan Institute of Urology, Kunming 650101, China.

Mitochondria coordinated a lot of vital cellular processes of energy production and distribution. Change of mitochondrial functions has been implicated in cancer progression. The present study aims to investigate the involvement of mitochondria dynamics in LASS2 induced invasion and chemoresistance of bladder cancer cells. J82 and BIU87 cell lines were used for LASS2 plasmid transfection while siRNA knockdown was carried out in 5637 cell line. Matrigel invasion assay and Annexin V/PI staining demonstrated that LASS2 negatively regulated cancer cell invasion and chemoresistance. JC-1 staining suggested that LASS2 overexpression downregulated mitochondrial membrane potential. Mitotracker staining showed that LASS2 induced mitochondrial fusion and inhibited mitochondrial fission. In addition, LASS2 overexpression downregulated expression of mitochondrial fission protein p-Drp1 Drp1 and Fis1. While depletion of LASS2 exhibited the opposite effects. Drp1 inhibitor Mdivi abolished invasion and chemoresistance induced by LASS2 siRNA. Furthermore, we found that LASS2 overexpression could inhibit phosphorylation of ERK, which act upstream of Drp1. ERK inhibitor PD98059 suppressed Drp1 phosphorylation and abrogated the effects of LASS2 depletion. In conclusion, the present study demonstrated that LASS2 inhibits bladder cancer invasion and chemoresistance through regulation of ERK-Drp1 induced mitochondrial dynamics.
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http://dx.doi.org/10.7150/jca.23087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868169PMC
February 2018

Risk and cause of interval colorectal cancer after colonoscopic polypectomy.

Digestion 2012 8;86(2):148-54. Epub 2012 Aug 8.

Department of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical College, Huhhot, PR China.

Background: To investigate the cause and risk of interval colorectal cancer (ICC) in patients undergoing surveillance colonoscopy within 5 years after colonoscopic polypectomy.

Patients And Methods: We retrospectively analyzed data (endoscopy, pathology, demography) of patients who received surveillance colonoscopy within 5 years after colonoscopic polypectomy.

Results: Among 1,794 patients undergoing surveillance colonoscopy within 5 years after colonoscopic polypectomy, 14 suffered from ICC. The mean follow-up time was 2.67 years and the incidence density of ICC was 2.9 cases per 1,000 person-years. 50% of ICCs were found in patients in whom adenomas had been incompletely removed by endoscopic therapy, 36% were missed cancers, and 14% were new cancers. Age >60 years (OR 2.97, 95% CI 2.31-3.82) was significantly associated with interval cancer on the surveillance colonoscopy as were advanced adenoma (OR 1.28, 95% CI 1.01-1.62), the presence of villous (HR 1.38, 95% CI 1.03-1.85) and high-grade dysplasia (OR 1.61, 95% CI 1.07-2.42).

Conclusions: Among patients undergoing surveillance colonoscopy within 5 years after polypectomy, the incidence density of ICC was 2.9 cases per 1,000 person-years. The majority of interval cancers originated from incomplete resection of advanced adenomas and missed cancers, which can be prevented by improving endoscopic techniques and selecting an appropriate follow-up time interval.
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http://dx.doi.org/10.1159/000338680DOI Listing
January 2013

Five-year risk of colorectal neoplasia after normal baseline colonoscopy in asymptomatic Chinese Mongolian over 50 years of age.

Int J Colorectal Dis 2012 Dec 5;27(12):1651-6. Epub 2012 Jul 5.

Department of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical College, Huhhot, Inner Mongolia Autonomous Region, 010050, People's Republic of China.

Background: After normal colonoscopy, the 5-year risk of colorectal neoplasia is sufficiently low for asymptomatic people over 50 years of age. In China, the incidence of colorectal carcinoma of Mongolian people is higher than that of Han people. The aim of this study was to assess the 5-year risk of colorectal neoplasia after normal colonoscopy in asymptomatic Chinese Mongolian population.

Patients And Methods: A cohort of asymptomatic Chinese Mongolian people (≥50 years old) were recruited and followed up with colonoscopy 5 years after colonoscopy. Baseline colonoscopy and follow-up colonoscopy findings were categorized based on the most advanced lesions: no adenoma, nonadvanced adenoma, and advanced adenoma. Five-year risk of colorectal neoplasia in these people was assessed according to the rates of no baseline adenoma and advanced adenoma at the end of 5 years.

Results: A total of 480 of the 538 recruited people underwent follow-up colonoscopy at the end of 5 years. In people with no baseline adenoma, 27.3 % (82/301) was found to have any adenoma, and 1.7 % had advanced adenoma at follow-up colonoscopy. The risk of an advanced adenoma did not differ significantly between people with no adenoma at baseline and those with nonadvanced adenoma (relative risk (RR), 1.06; 95 % confidence interval (CI), 0.19-6.07). Advanced adenoma at baseline colonoscopy was the independent risk factor for advanced adenoma recurrence, compared with no adenoma at baseline (RR, 8.25; 95 % CI, 1.90-35.77).

Conclusion: The risk of advanced adenoma is low 5 years after the normal baseline colonoscopy, even in asymptomatic Chinese Mongolian population over 50 years of age.
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http://dx.doi.org/10.1007/s00384-012-1516-5DOI Listing
December 2012

Risk of ulcerative colitis-associated colorectal cancer in China: a multi-center retrospective study.

Dig Dis Sci 2012 Feb 22;57(2):503-7. Epub 2011 Sep 22.

Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People's Republic of China.

Background: The number of patients with ulcerative colitis (UC) in China has increased in the past 10 years. Thus, it is anticipated that the incidence of UC-associated colorectal cancer (UC-CRC) will also increase. However, the risk of CRC in UC patients is still unknown in Chinese. The aim of this study was to identify the risk and risk factors of UC-CRC in Chinese.

Methods: A total of 3,922 patients with UC were retrospectively collected from five central teaching hospitals in China, in which high-quality endoscopic and histological diagnoses were available from 1998 to 2009. The database of the UC and UC-associated CRC patients was evaluated.

Results: CRC was diagnosed 34 in patients, and the overall prevalence of CRC in patients with UC was 0.87%. The cumulative risk of developing CRC after a disease duration of 10 years was 1.15% (95% confidence interval [CI] 0.71-1.84%); 20 years, 3.56% (95% CI 2.14-5.89%); and 30 years, 14.36% (95% CI 7.57-26.3%). Longer disease duration, extensive colitis, and dysplasia found in the biopsy specimen were identified as risk factors for developing CRC. 5-ASA use was identified as a protective factor of UC-CRC.

Conclusions: The period prevalence of CRC was lower than that reported from the West. However, the cumulative risk was found to be comparable to that of Western countries, which suggests that the period prevalence of UC-CRC in China may be growing in the future.
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http://dx.doi.org/10.1007/s10620-011-1890-9DOI Listing
February 2012

The comparison of the clinical manifestations and risk factors of colorectal cancer and adenomas: results from a colonoscopy-based study in southern Chinese.

Int J Colorectal Dis 2010 Nov 3;25(11):1343-51. Epub 2010 Aug 3.

Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.

Background And Aims: Colorectal cancer (CRC) is one of the most common gastrointestinal tumors in the world. This study aimed to compare the clinical manifestations and risk factors of CRC and adenomas in native patients of Guangzhou.

Methods: Patients who underwent colonoscopy for the first time at Nanfang Hospital between July 2008 and July 2009 were recruited. Data on demographic information, main clinical manifestations, results of endoscopies and pathology, and possible risk factors of colorectal tumor were collected. Chi-square test and logistic regression were used to compare the clinical characteristics and risk factors for CRC and adenomas.

Results: Hematochezia and body weight loss were more frequent in proximal and distal CRC groups, respectively (P ≤  0.05). Older age [odds ratio (OR), 1.079; 95% confidence interval (CI), 1.065-1.093], smoking status (OR, 1.712; 95% CI, 1.158-2.531), BMI =18.5-24.9 and  ≥ 25.0 (OR, 2.384; 95% CI, 1.250-4.549; OR, 2.162; 95% CI, 1.044-4.478, respectively) were significant risk factors for advanced adenoma, while female (OR, 0.638; 95% CI, 0.429-0.949) and using aspirin (OR, 0.188; 95% CI, 0.042-0.845) were significant protective factors. Hyperlipemia (OR, 0.109; 95% CI, 0.013-0.886) was identified as a protective factor for proximal CRC. Smoking (OR, 1.717; 95% CI, 1.093-2.696), drinking (OR, 1.817; 95% CI, 1.145-2.883), DM history (OR, 2.204; 95% CI, 1.044-4.652) were identified as independent risk factors for distal CRC, and using aspirin (OR, 0.190; 95% CI, 0.043-0.840) was a protective factor. Drinking (OR, 3.288; 95% CI, 1.546-6.994; OR, 1.862; 95% CI, 1.037-3.343, respectively) was an independent risk factor for both poorly to moderately differentiated CRC and well-differentiated CRC. Besides, DM (OR, 3.761; 95% CI, 1.615-8.762) and hypertension (OR, 0.384; 95% CI, 0.178-0.828) were identified as independent risk factor and protective factor for well-differentiated CRC, respectively.

Conclusions: Hematochezia and body weight loss were representative manifestations for distal and proximal CRC, respectively. For southern Chinese the most important influential factors for colorectal tumor are age, smoking, drinking, nutritional state, DM, hypertension, and the use of aspirin.
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http://dx.doi.org/10.1007/s00384-010-1030-6DOI Listing
November 2010

Recurrence and surveillance of colorectal adenoma after polypectomy in a southern Chinese population.

J Gastroenterol 2010 Aug 25;45(8):838-45. Epub 2010 Mar 25.

Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.

Background And Aim: Repeat colonoscopy is often performed within a short time after polypectomy due to the fear that colorectal adenomas were missed during the initial colonoscopy or that new adenomas have developed. The aim of this study was to estimate the actual recurrence rate of adenoma and its association with the length of the surveillance interval after polypectomy in a southern Chinese population.

Methods: A total of 1,356 patients undergoing endoscopic polypectomy and completing three or more surveillence colonoscopies between 1976 and 2007 were retrospectively analyzed. The recurrence rates of adenoma and advanced adenoma and surveillance intervals after polypectomy were identified based on the features of adenomas detected on initial colonoscopy.

Results: The recurrence rates of advanced adenoma in patients with non-advanced adenoma on the initial colonoscopy were 0.9, 3.9, 5.8, and 29.2% during surveillance intervals of 1-3, 3-5, 5-10, and 10-20 years post-initial colonoscopy; for patients with advanced adenoma on the initial colonoscopy, the recurrence rates were 3.8, 13.1, 34.7, and 52.0% during the same surveillance intervals, respectively. Older age (p < 0.05 for trend) and male sex [hazard ratio (HR) 2.11, 95% confidence interval (CI) 1.27-3.53] were significantly associated with recurrence for advanced adenoma, as were the size and number of baseline adenoma (p < 0.05 for trend), tubulovillous, villous adenoma (HR 2.57, 95% CI 1.24-5.32), and high-grade dysplasia (HR 1.61, 95% CI 1.07-2.42). When 5% of patients had recurring advanced adenoma, the surveillance interval was estimated to be 6.9 (95% CI 6.3-12.2) years in the low-risk group and 3.0 (95% CI 2.7-3.2) years in the high-risk group.

Conclusions: Among our patient group, the recurrence of advanced adenoma after polypectomy increased with the length of the surveillance interval. Based on our results, a 3-year follow-up of patients after polypectomy could be effective in preventing the recurrence of advanced adenoma in high-risk patients.
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http://dx.doi.org/10.1007/s00535-010-0227-3DOI Listing
August 2010

Clinicopathologic features and endoscopic mucosal resection of laterally spreading tumors: experience from China.

Int J Colorectal Dis 2009 Dec 18;24(12):1441-50. Epub 2009 Jun 18.

Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.

Background: Laterally spreading tumors (LSTs) are being increasingly reported nowadays in Japan and the western countries with the application of magnification chromoendoscopy. The aim of this study was to analyze the clinicopathologic features of LSTs and to assess the outcome and safety of endoscopic mucosal resection (EMR) in China.

Patients And Methods: One hundred nine patients with LSTs who underwent magnification chromoendoscopy were studied retrospectively. Clinicopathological features of 111 LSTs were analyzed. The efficacy and safety of EMR was assessed in 79 LSTs based on the outcome of follow-up colonoscopy and resection-related complications.

Results: A total of 111 LSTs were diagnosed in 109 patients, including 89 (80%) laterally spreading tumor-granular (LST-G) type and 22 (20%) laterally spreading tumor-non-granular (LST-NG) type. There was significant difference in the dominant pit pattern between LST-G type and LST-NG type (p < 0.001). Type IV pit pattern (62%) was the main crypt pattern in LST-G type; whereas, type IIIL (50%) and type V pit pattern (36%) were predominant crypt patterns in LST-NG type. EMR was performed for 103 lesions. Six of the nine lesions with type V(I) pit pattern were completely resected by EMR. Eleven (14%) local recurrent lesions were detected in 79 follow-up lesions and were treated successfully during the follow-up.

Conclusions: The type of dominant pit pattern was different between LST-G type and LST-NG type. Many LSTs with a type V(I) pit pattern can be completely resected by EMR. EMR technique is a safe and efficacious treatment method for LST.
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http://dx.doi.org/10.1007/s00384-009-0749-4DOI Listing
December 2009

Influence of Mg doping on GaN nanowires.

Chemphyschem 2009 Feb;10(3):571-5

Institute of Semiconductors, College of Physics and Electronics, Shandong Normal University, Jinan 250014, China.

Magnesium-doped GaN nanowires with different dopant concentrations are synthesized by ammoniating Ga(2)O(3) thin films doped with Mg at 900 degrees C. Scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), high-resolution transmission electron microscopy (HRTEM), and room-temperature photoluminescence (PL) are employed to characterize the influences on the morphology, structure, crystallinity, and optical properties of Mg-doped GaN nanowires. The results demonstrate that the nanowires are single-crystalline with hexagonal wurzite structure. GaN nanowires doped with 5 atom % of Mg have the best morphology and crystallinity with a single-crystalline structure, and at this composition the PL spectrum with the strongest UV peak is observed. The growth mechanism of crystalline GaN nanowires is discussed briefly.
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http://dx.doi.org/10.1002/cphc.200800529DOI Listing
February 2009